LOCUS       DACEDM010000001       104613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEDM010000001 DACEDM010000000
VERSION     DACEDM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 104613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.001"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            400..1423
                     /locus_tag="IWO50_000001"
                     /pseudo
     CDS             400..1423
                     /locus_tag="IWO50_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1766..2022
                     /locus_tag="IWO50_000002"
                     /pseudo
     CDS             1766..2022
                     /locus_tag="IWO50_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2409..2636
                     /locus_tag="IWO50_000003"
     CDS             2409..2636
                     /locus_tag="IWO50_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0357163.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2652..3818
                     /locus_tag="IWO50_000004"
     CDS             2652..3818
                     /locus_tag="IWO50_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0357164.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            4053..5096
                     /gene="hrcA"
                     /locus_tag="IWO50_000005"
     CDS             4053..5096
                     /gene="hrcA"
                     /locus_tag="IWO50_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0357165.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            5190..5753
                     /gene="grpE"
                     /locus_tag="IWO50_000006"
     CDS             5190..5753
                     /gene="grpE"
                     /locus_tag="IWO50_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0357166.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5804..7633
                     /gene="dnaK"
                     /locus_tag="IWO50_000007"
     CDS             5804..7633
                     /gene="dnaK"
                     /locus_tag="IWO50_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0357167.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7784..8950
                     /gene="dnaJ"
                     /locus_tag="IWO50_000008"
     CDS             7784..8950
                     /gene="dnaJ"
                     /locus_tag="IWO50_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0357168.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(9318..10055)
                     /locus_tag="IWO50_000009"
     CDS             complement(9318..10055)
                     /locus_tag="IWO50_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357169.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(10057..11559)
                     /locus_tag="IWO50_000010"
     CDS             complement(10057..11559)
                     /locus_tag="IWO50_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0357170.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11818..13812
                     /gene="uvrB"
                     /locus_tag="IWO50_000011"
     CDS             11818..13812
                     /gene="uvrB"
                     /locus_tag="IWO50_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0357171.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13822..16638
                     /gene="uvrA"
                     /locus_tag="IWO50_000012"
     CDS             13822..16638
                     /gene="uvrA"
                     /locus_tag="IWO50_000012"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0357172.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16901..18166
                     /locus_tag="IWO50_000013"
     CDS             16901..18166
                     /locus_tag="IWO50_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0357173.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            18170..18901
                     /locus_tag="IWO50_000014"
     CDS             18170..18901
                     /locus_tag="IWO50_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0357174.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            19036..19920
                     /gene="rapZ"
                     /locus_tag="IWO50_000015"
     CDS             19036..19920
                     /gene="rapZ"
                     /locus_tag="IWO50_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0357175.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19917..20915
                     /locus_tag="IWO50_000016"
     CDS             19917..20915
                     /locus_tag="IWO50_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0357176.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20949..21884
                     /gene="whiA"
                     /locus_tag="IWO50_000017"
     CDS             20949..21884
                     /gene="whiA"
                     /locus_tag="IWO50_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0357177.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            22452..23108
                     /locus_tag="IWO50_000018"
     CDS             22452..23108
                     /locus_tag="IWO50_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357178.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            23287..23852
                     /locus_tag="IWO50_000019"
                     /pseudo
     CDS             23287..23852
                     /locus_tag="IWO50_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24551..25072)
                     /locus_tag="IWO50_000020"
     CDS             complement(24551..25072)
                     /locus_tag="IWO50_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0357179.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            25270..28950
                     /gene="nifJ"
                     /locus_tag="IWO50_000021"
     CDS             25270..28950
                     /gene="nifJ"
                     /locus_tag="IWO50_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0357180.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            29139..30026
                     /locus_tag="IWO50_000022"
     CDS             29139..30026
                     /locus_tag="IWO50_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0357181.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            30023..31192
                     /locus_tag="IWO50_000023"
     CDS             30023..31192
                     /locus_tag="IWO50_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357182.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            31216..32571
                     /locus_tag="IWO50_000024"
     CDS             31216..32571
                     /locus_tag="IWO50_000024"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0357183.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(32643..34448)
                     /gene="glmS"
                     /locus_tag="IWO50_000025"
     CDS             complement(32643..34448)
                     /gene="glmS"
                     /locus_tag="IWO50_000025"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0357184.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            34858..35148
                     /locus_tag="IWO50_000026"
     CDS             34858..35148
                     /locus_tag="IWO50_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357185.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            35161..36186
                     /gene="sppA"
                     /locus_tag="IWO50_000027"
     CDS             35161..36186
                     /gene="sppA"
                     /locus_tag="IWO50_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0357186.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            36189..36848
                     /locus_tag="IWO50_000028"
     CDS             36189..36848
                     /locus_tag="IWO50_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0357187.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            37005..37937
                     /locus_tag="IWO50_000029"
     CDS             37005..37937
                     /locus_tag="IWO50_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357188.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            38091..39380
                     /locus_tag="IWO50_000030"
     CDS             38091..39380
                     /locus_tag="IWO50_000030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0357189.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            39627..40877
                     /gene="clpX"
                     /locus_tag="IWO50_000031"
     CDS             39627..40877
                     /gene="clpX"
                     /locus_tag="IWO50_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0357190.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            40960..41547
                     /locus_tag="IWO50_000032"
     CDS             40960..41547
                     /locus_tag="IWO50_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0357191.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            41912..43240
                     /locus_tag="IWO50_000033"
     CDS             41912..43240
                     /locus_tag="IWO50_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357192.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            43251..43460
                     /locus_tag="IWO50_000034"
     CDS             43251..43460
                     /locus_tag="IWO50_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357193.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            43619..44371
                     /locus_tag="IWO50_000035"
     CDS             43619..44371
                     /locus_tag="IWO50_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0357194.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            44368..45282
                     /gene="pfkB"
                     /locus_tag="IWO50_000036"
     CDS             44368..45282
                     /gene="pfkB"
                     /locus_tag="IWO50_000036"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0357195.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            45300..47231
                     /locus_tag="IWO50_000037"
     CDS             45300..47231
                     /locus_tag="IWO50_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0357196.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(47342..48901)
                     /locus_tag="IWO50_000038"
     CDS             complement(47342..48901)
                     /locus_tag="IWO50_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0357197.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            49056..51290
                     /gene="pcrA"
                     /locus_tag="IWO50_000039"
     CDS             49056..51290
                     /gene="pcrA"
                     /locus_tag="IWO50_000039"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0357198.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            51307..53343
                     /gene="ligA"
                     /locus_tag="IWO50_000040"
     CDS             51307..53343
                     /gene="ligA"
                     /locus_tag="IWO50_000040"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0357199.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            53449..53754
                     /gene="gatC"
                     /locus_tag="IWO50_000041"
     CDS             53449..53754
                     /gene="gatC"
                     /locus_tag="IWO50_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0357200.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            53754..55226
                     /gene="gatA"
                     /locus_tag="IWO50_000042"
     CDS             53754..55226
                     /gene="gatA"
                     /locus_tag="IWO50_000042"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0357201.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            55223..56653
                     /gene="gatB"
                     /locus_tag="IWO50_000043"
     CDS             55223..56653
                     /gene="gatB"
                     /locus_tag="IWO50_000043"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0357202.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            56663..57718
                     /locus_tag="IWO50_000044"
     CDS             56663..57718
                     /locus_tag="IWO50_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0357203.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            57829..59202
                     /gene="rlmD"
                     /locus_tag="IWO50_000045"
     CDS             57829..59202
                     /gene="rlmD"
                     /locus_tag="IWO50_000045"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0357204.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            59205..59333
                     /locus_tag="IWO50_000046"
     CDS             59205..59333
                     /locus_tag="IWO50_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0357205.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            59773..59961
                     /locus_tag="IWO50_000047"
     CDS             59773..59961
                     /locus_tag="IWO50_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0357206.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            60039..61052
                     /locus_tag="IWO50_000048"
     CDS             60039..61052
                     /locus_tag="IWO50_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0357207.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(61119..62153)
                     /locus_tag="IWO50_000049"
     CDS             complement(61119..62153)
                     /locus_tag="IWO50_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0357208.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            62268..64493
                     /locus_tag="IWO50_000050"
     CDS             62268..64493
                     /locus_tag="IWO50_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0357209.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            64511..66394
                     /locus_tag="IWO50_000051"
     CDS             64511..66394
                     /locus_tag="IWO50_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0357210.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            66375..67349
                     /locus_tag="IWO50_000052"
     CDS             66375..67349
                     /locus_tag="IWO50_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0357211.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            68008..69030
                     /gene="aroF"
                     /locus_tag="IWO50_000053"
     CDS             68008..69030
                     /gene="aroF"
                     /locus_tag="IWO50_000053"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0357212.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            69063..70127
                     /gene="aroB"
                     /locus_tag="IWO50_000054"
     CDS             69063..70127
                     /gene="aroB"
                     /locus_tag="IWO50_000054"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0357213.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            70129..71295
                     /gene="aroC"
                     /locus_tag="IWO50_000055"
     CDS             70129..71295
                     /gene="aroC"
                     /locus_tag="IWO50_000055"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0357214.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            71312..72403
                     /locus_tag="IWO50_000056"
     CDS             71312..72403
                     /locus_tag="IWO50_000056"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0357215.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            72416..73711
                     /gene="aroA"
                     /locus_tag="IWO50_000057"
     CDS             72416..73711
                     /gene="aroA"
                     /locus_tag="IWO50_000057"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0357216.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            73704..74213
                     /locus_tag="IWO50_000058"
     CDS             73704..74213
                     /locus_tag="IWO50_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0357217.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            74210..75046
                     /gene="pheA"
                     /locus_tag="IWO50_000059"
     CDS             74210..75046
                     /gene="pheA"
                     /locus_tag="IWO50_000059"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0357218.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(75109..76845)
                     /locus_tag="IWO50_000060"
     CDS             complement(75109..76845)
                     /locus_tag="IWO50_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0357219.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(76842..77771)
                     /locus_tag="IWO50_000061"
     CDS             complement(76842..77771)
                     /locus_tag="IWO50_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0357220.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            complement(77771..78349)
                     /locus_tag="IWO50_000062"
     CDS             complement(77771..78349)
                     /locus_tag="IWO50_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0357221.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            78622..79908
                     /locus_tag="IWO50_000063"
     CDS             78622..79908
                     /locus_tag="IWO50_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0357222.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            80045..80914
                     /locus_tag="IWO50_000064"
     CDS             80045..80914
                     /locus_tag="IWO50_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0357223.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            80911..81798
                     /locus_tag="IWO50_000065"
     CDS             80911..81798
                     /locus_tag="IWO50_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0357224.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            81965..82648
                     /locus_tag="IWO50_000066"
     CDS             81965..82648
                     /locus_tag="IWO50_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0357225.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            82645..83418
                     /locus_tag="IWO50_000067"
     CDS             82645..83418
                     /locus_tag="IWO50_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0357226.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            83415..83600
                     /locus_tag="IWO50_000068"
                     /pseudo
     CDS             83415..83600
                     /locus_tag="IWO50_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735288.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
     gene            83697..84365
                     /locus_tag="IWO50_000069"
     CDS             83697..84365
                     /locus_tag="IWO50_000069"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0357227.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            84429..85052
                     /locus_tag="IWO50_000070"
     CDS             84429..85052
                     /locus_tag="IWO50_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0357228.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            85108..85494
                     /locus_tag="IWO50_000071"
     CDS             85108..85494
                     /locus_tag="IWO50_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0357229.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            85707..87098
                     /locus_tag="IWO50_000072"
     CDS             85707..87098
                     /locus_tag="IWO50_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0357230.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            87445..88092
                     /locus_tag="IWO50_000073"
     CDS             87445..88092
                     /locus_tag="IWO50_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0357231.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            88485..91130
                     /locus_tag="IWO50_000074"
     CDS             88485..91130
                     /locus_tag="IWO50_000074"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0357232.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            91440..92753
                     /locus_tag="IWO50_000075"
     CDS             91440..92753
                     /locus_tag="IWO50_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0357233.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            92743..93396
                     /locus_tag="IWO50_000076"
     CDS             92743..93396
                     /locus_tag="IWO50_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0357234.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            93451..94122
                     /gene="radC"
                     /locus_tag="IWO50_000077"
     CDS             93451..94122
                     /gene="radC"
                     /locus_tag="IWO50_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ0357235.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(94017..95369)
                     /locus_tag="IWO50_000078"
     CDS             complement(94017..95369)
                     /locus_tag="IWO50_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0357236.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(95435..96724)
                     /locus_tag="IWO50_000079"
     CDS             complement(95435..96724)
                     /locus_tag="IWO50_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0357237.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(96811..97515)
                     /locus_tag="IWO50_000080"
     CDS             complement(96811..97515)
                     /locus_tag="IWO50_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ0357238.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            97715..97981
                     /locus_tag="IWO50_000081"
     CDS             97715..97981
                     /locus_tag="IWO50_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0357239.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(98149..98379)
                     /locus_tag="IWO50_000082"
     CDS             complement(98149..98379)
                     /locus_tag="IWO50_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0357240.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            98451..98606
                     /locus_tag="IWO50_000083"
     CDS             98451..98606
                     /locus_tag="IWO50_000083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357241.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(98915..99139)
                     /locus_tag="IWO50_000084"
     CDS             complement(98915..99139)
                     /locus_tag="IWO50_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0357242.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            99406..99741
                     /locus_tag="IWO50_000085"
     CDS             99406..99741
                     /locus_tag="IWO50_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357243.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            99974..100915
                     /locus_tag="IWO50_000086"
     CDS             99974..100915
                     /locus_tag="IWO50_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0357244.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            100917..101807
                     /locus_tag="IWO50_000087"
     CDS             100917..101807
                     /locus_tag="IWO50_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ0357245.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            101850..102650
                     /locus_tag="IWO50_000088"
     CDS             101850..102650
                     /locus_tag="IWO50_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0357246.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            102665..103516
                     /locus_tag="IWO50_000089"
     CDS             102665..103516
                     /locus_tag="IWO50_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0357247.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            103513..103830
                     /locus_tag="IWO50_000090"
     CDS             103513..103830
                     /locus_tag="IWO50_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357248.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            103823..104005
                     /locus_tag="IWO50_000091"
     CDS             103823..104005
                     /locus_tag="IWO50_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357249.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            104013..104432
                     /locus_tag="IWO50_000092"
     CDS             104013..104432
                     /locus_tag="IWO50_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0357250.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            104429..>104613
                     /locus_tag="IWO50_000093"
     CDS             104429..>104613
                     /locus_tag="IWO50_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357251.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDP"
BASE COUNT        34502 a        18166 c        22364 g        29581 t
ORIGIN      
        1 aaacacagct aaaaattagt tgtgtttttt attaggttta cttttctggt ataaaaggaa
       61 ctagctactt tttcaaaatt tctaaatcat tcaatatgta gcgcttacta aattattcgc
      121 cataaaattt tataattgga tcactcattg ttttacctaa gtcatattta agtaataacc
      181 ggtgttttta cataagaaga cttcgggtat tttttagttt gattagctct gatataccta
      241 taaagaatat gctaaggtaa gaatagtaaa aattcacata gatgaggtat aataatgata
      301 tttttatata ttacagataa cttgacacaa aatgagtttt tttcgatttt tcaagttatc
      361 taatgcaatt atattgatat cattaggagc tgcattgtta tgaatccaac attgattact
      421 aagaaagaat tactaaaaaa attagatatt tctactggtg tattagctaa tttaattaga
      481 aatggcatgc caaaagaggg agaaatgttt aatcttgata agattattac atggcgagaa
      541 aattggtcta aaaacatatt aggtgagtta gaagttggta gagtatatac taacaaagaa
      601 atttcagaga agtttaaatg ttccaaacag ggtggaatga gaagatctca tcaaactaat
      661 actcttgtac tgtttagtga tcaaacagga agtaatgttt ataaagataa gtggttaaat
      721 ggtattttac aatataccgg tatggggcta aaaggggatc aagtattgga taagaatcaa
      781 aataaagttt tagccaattc aaaaagtaat tttgtaaaga ttcatttatt tgaaacattt
      841 aaaccaaaag aacacacata tctgggagaa gtatatttag caggacaaat atatactgtt
      901 aatgaaaaag atagttcggg caacagcaga aaagtatata aatttccatt agctttaata
      961 aatcaagagc aattgattga agataaagat atataaccag gaagaaaatc aaactagaca
     1021 tattcgtaat ttatctaatg ctaaattaga ggaagaagca agaaaagtta gtaattataa
     1081 taaaaaaatg gctgcaaaat atgttaacga agaggagttt tgcttctcaa aaacaaaaat
     1141 atataaacgt aatcccttcg tatctgaata tgttaaacga cttgcaaaag gtatttgtca
     1201 actttgccaa gaaaaagggc catttataaa agacggtgta ccttatttac attgtcatca
     1261 tatagaatat ttgtcgcaag gtggaaaaga tgtgattgaa aactgtatcg cgttatgtcc
     1321 caattgtcat gctagaattc atgaacttga gttacctgac gataaagaaa aattgctgaa
     1381 gatcgttgaa aaaagagaaa atagcttttc taaaataccg taatttattt ttataggaca
     1441 aataagctaa tgtgagctat gccttctata taagactaag ttctattata agtaacgaag
     1501 taattctcgt tccttcctgt agtaatattt tttaaataac ctataggctt cttcaaaagt
     1561 acatcctcca gtaaattcta agtactggag gattcttttt ctagtgttgc tttactcatg
     1621 aaaaagtagt tggctacttc atcaaaattt ctgaactttt ttaatgtata ataaagagct
     1681 cgatcattta ctacaaggat gctagctcct acattttcac agttttttag caagtaaatt
     1741 tttctaaaat cacatcagga aatgcatgag attctttaag agaggcaatt atagggctat
     1801 gattacggtt cgtgtaagtg acgccaaaaa agaatggctt tcttatatgg ctgaatttta
     1861 tgggattact ttatctgaac ttctaaaaaa ctattcgatg gaacaactag aagatgaata
     1921 caatcgtcaa acggctgaaa ttgcccataa acattggata agaagtggga aaacaacagt
     1981 attcatggaa gaatccttaa tgaatttggt gggctggaat gacctgccgt ttaaaattca
     2041 cgcccgaaat tcagaagcag ctctgattgg caaatacgtg tattggtaga gatagaagat
     2101 aatcaattcg ttgttatcgc tattcaagta gggcaccgaa gaaatgtcta tgattaagag
     2161 actgtttatt tttacaaaaa aataataatt ggaatctagt taaaactctt gaagtgagag
     2221 atttgactag atttttttat gaataaaata aggacaaaat tgtttaggaa gaatcatgag
     2281 tgtaaaaaaa tcatccaaga agtcgattct tggatttttt agtggatttg ttcgccaaat
     2341 acgtttagta tgcgtatatg agaggttgta agcaaagcgt tgtggtctga gcacttaagg
     2401 aggaatctgt gaaaatagct tttcatattt ttgcagattt tgacttaatg ccgaaggtca
     2461 gctttgcgat taccgtttat cgagaggctg taaaagcacc gtgtagctcc gatgacaata
     2521 agcaagcatt tttagagcta ggtttccttg attctaaata tgcggcttat ttcaatgagt
     2581 tggtgcttga acgcgatttt aagaaggtcg aaaacaggca cgacttttcg acgtaaggag
     2641 aaagaatttc aatgatagaa acaataaatc ccgcagaaaa caaggtttct gcgtacattc
     2701 atatcccatt ttgcgagcat atctgctatt actgtgactt taataaagtt tttttagaag
     2761 ggcagccagt agatgagtat gtggaaatgc tgctgaatga aatgagatta atgctggaaa
     2821 gtcatcctac agcgaatttg gaaacacttt atgtaggagg cggaacacca acttctttat
     2881 ctgctaaaca acttgatcga ctattatcag gtgctagaga gatcctgcct tttcaaaatg
     2941 gtaatgagtt tactgtagaa gcgaatcctg gtgatttgac ccgtgaaaaa cttcaagtga
     3001 tgaaaaacta tggcgtcaat cgtttatcca tgggagtgca gacatttgac aatcgcttat
     3061 tgaaaaaaat tggacgtaaa catactgcag aagatgtgta tcaaacaatg caatttttag
     3121 aagcagaaaa ttttaccaat gtaagcatcg atttgattta tgcattaccg ggacaaacac
     3181 tagaaggata tcgggatact ttggaaaaag ctttggcttt ggatctgcct cactattctt
     3241 tgtactcatt gatcctcgaa aacaaaacaa tgttcatgaa ctgggtacga caaggaagac
     3301 tcgaactgcc tgatcaggaa acggaaacca gaatgtttga agagacgatt gaagcaatgg
     3361 aaaagcatgg acgtcaccaa tatgaaataa gtaactttgg gctctccgga catgaatctc
     3421 aacataacct catgtactgg aacaatgacc actattttgg gtttggagct ggtgctagcg
     3481 gttatcttgg agatattcgt taccgcaaca aaggaccaat ccaacactat ttacgcccat
     3541 tacgagcaaa tgaacgcccg gtattggaag aagaaaaatt gacacgaaaa aatcaaatag
     3601 aagaagaaat gttcttagga ctaagaaaaa aaacagggat tactaaaaaa catttctatg
     3661 atcgctatca aaaaacaatt gaatcggtgt acggtgatgt gctaagggaa ttagagcaag
     3721 aagggttact tgttaatgaa gaagaccgtg tttatttgac accaagaggg acttttttgg
     3781 ggaacaatgt gtttgagcgc tttttatttg aaaaatagtt tgaagaatat ttgtttttta
     3841 tcgaaacttg aaaagaaaaa gtttagaaag tttgacaatg aggtattgaa gtattgatat
     3901 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
     3961 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgaa ttagcactta
     4021 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
     4081 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattaa
     4141 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
     4201 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
     4261 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
     4321 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
     4381 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
     4441 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
     4501 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
     4561 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
     4621 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaga
     4681 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagaaaagg
     4741 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
     4801 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
     4861 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
     4921 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
     4981 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
     5041 aactagcagc taaacttgct gattactacc gctcgcttga cttgtcaaac tcgtaatgtg
     5101 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
     5161 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
     5221 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
     5281 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
     5341 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
     5401 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
     5461 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
     5521 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
     5581 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
     5641 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
     5701 acaaattaca tgatcgcgtg ttaagaccaa ctatggtcat tgttgcacaa taattgtgcg
     5761 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
     5821 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
     5881 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
     5941 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
     6001 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
     6061 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
     6121 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
     6181 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
     6241 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
     6301 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
     6361 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
     6421 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
     6481 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
     6541 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
     6601 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
     6661 tacaaaagta ccagtacgtc aagcgttaaa agatgcaggt ctgtcacaat cagaaatcga
     6721 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
     6781 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
     6841 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
     6901 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
     6961 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
     7021 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
     7081 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
     7141 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
     7201 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
     7261 ggtgaaagat gcagaagcaa acgctgaagc agataaagct cgtaaagagg aagttgacct
     7321 acgtaatgat gtagacgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
     7381 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgtgacgaat taaaagccgc
     7441 tgttgaagca aacaacatcg aggaaatgaa aactaaacgc gatgcattga acgaaatcgt
     7501 tcaaaacttg actgttaaac tatacgaaca agctgcacaa caacaagctc aagaaaatcc
     7561 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
     7621 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
     7681 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
     7741 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
     7801 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
     7861 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
     7921 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
     7981 cgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
     8041 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
     8101 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
     8161 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
     8221 caagtataat cgggaagaaa tctgtcatac ttgtggtgga aacggtgcaa aaccaggcac
     8281 acaaccaact acttgtcata aatgtcatgg tgcaggtacg atcaatgtag aacgccaaac
     8341 gccacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggca cagggaaaga
     8401 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
     8461 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
     8521 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtttttc gtgtagaaga
     8581 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
     8641 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
     8701 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
     8761 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
     8821 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
     8881 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
     8941 gcggaagtag tttttctagt aaaaatataa agtagacttg cccaaatttt ctatttctag
     9001 taaaaacgct aagtcattaa taattaaaaa ccagacgaac aatcatacgt tcgcctggtt
     9061 tttttgtagt tgtcatgttt gtaccaacaa aaaagccagc tttagaataa gataatcatc
     9121 atagtttctg aaggtggtat cgactatgta cttgatatcg tcaatgaacc gatggtactt
     9181 tcttatgcga tggaacaggt agagcgattg cttgtttgtg caggagaaca agcgatgcgg
     9241 ctggtacagt taagtgaaaa aacaagaaac taaaaatgcc cggtcgtcat gaagcgaccg
     9301 ggcatttttg gtttttatta aatatttaat actttattca agaaatcttt tgtccgttca
     9361 ttttgcggat tttcaaagat ttgttgaggc gttccgtctt ctaaaaagtt tccgtcgtca
     9421 atgaacatga cacgattggc cacttcttta gcgaagccca tttcgtgcgt aacgataacc
     9481 atcgtcatac cttgttctgc taactttttc atgacgttca agacatcacc gaccatttcg
     9541 gggtcgagtg cagatgtagg ctcatcaaaa agcatgatgt cagggttcat cgctagagca
     9601 cgagcaatag ccacacgctg cttttgtcca ccggaaagca tttcaggata catatctttt
     9661 ttatcttcta gaccaactgt ctgcagcaat ttgattgctt ttttctctgc ttcttctcta
     9721 gattctttct tcaggtccat cggtgctaaa atgatgtttt ccaaaataga aagatgaggg
     9781 aataggttaa agtgctggaa taccattccg atatgttgtc tcaccaagtt gatatctgtg
     9841 cttttatcca tcaagtgggc gccgtcaata ataatttcac cgtcagaagg ttcttctaaa
     9901 cgattcaagc aacgcaaaaa tgtacttttt cctgaacctg aagggccaat gatacagacg
     9961 acatctcctt cgttgatgga cacattgatg tcgtttaata ctgtgttatc gccgtatttt
    10021 ttgactaaat gctcaactaa gattttttct gccatcttac ttcaccttct tttctaaaac
    10081 ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta
    10141 aacatacgta ctttgtgttg tacgtgcaac aatgatctta ccagtttgca gcaattcgac
    10201 tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag ataacgaact gattgacgaa
    10261 tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg tccgattata
    10321 agaaagacct aaacttctgg aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc
    10381 tcgaacaatc tctgcgatat aagcacttgc gttaagagtc aaggtgataa caccagcaag
    10441 gaagtcagga atcgtaaatc caacgatacc aggcaaccca aagaaaataa agaacgcaag
    10501 aaccatcatt ggaattcctc ggatgatatc gacataaata ctagcaattg tgcgaagagc
    10561 gcgtattggt gctacactga ataatccaaa aatcacaccg attactatag ccaaagcaaa
    10621 ggagatcaaa gttaggagaa ttgttctcca aagtcctttc aacaatgctt gatagttgtt
    10681 ttggataagt ccaagaaatg ttgattcatc agcagtggaa gactcactgg aagtttctga
    10741 accatccgct acatattctt taataatctt atcgtattcg cctgttcttt ttaattcttt
    10801 caatccttca ttgaacatat taagaagctc aggattttgt ccttttttaa cggcaaatgc
    10861 atattttccg ccagattctt ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc
    10921 ataaccgatt accggataat catccatcat tgcatcgatt gcgccaactt ttaacccgtc
    10981 gtacaattga tcagcagcat caaaatattt gatagaatag ccatattcag attcatggtc
    11041 gcttaaaaat tcagcacttt cggtaccaac ttttgctccg actgtttttc ctttcaaatc
    11101 ttcataagac ttgatcgaag tatctccttt tttgacagct aattggatcc cgctatcaaa
    11161 gtaagaatca gaaaaatcat agcttttctt tcgttcatct gttacagtca ttccggcaat
    11221 cattccgtct gcttgtcctg attccaaagc ttgcaaagca gcagagaaac cacggtagtc
    11281 cattcggatg ttaaatcctt gcagtttggc gatgttttcc atcaatttta catcgatgcc
    11341 ttcgtactcg ccctttgcgt tttgaaattc aaatggagca aaagcagagt cactggcaat
    11401 tacgtatacg tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc
    11461 cgttgtagtg ttgtcagctt gtgcaacatg tccaaacgca agtgtcacaa gtcctgtcat
    11521 taagaaaaca aatgagtgaa ataaatgctt tcttttcata atcaacctcc taaatttcca
    11581 atattttatt attataggcg aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat
    11641 gaaaaaaatg cgagtttcta acattttatg ttataaaacg taacatgcat aataagataa
    11701 aaaagtggcg aaagtatgtt cggtgtggta taatttttta gtaaggaatg cctttgcttt
    11761 ccttttcttt ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg
    11821 attgagagag atacctcccg acactttgag cttgtctcga aataccaacc ggctggtgat
    11881 cagcctgaag cgattaacca attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc
    11941 ttattaggtg cgactggaac agggaaaaca tatacgattt ccaatcttat tgagaaagtg
    12001 aataaaccaa cactaattat tgcccataac aaaacattgg ctggtcagct gtacggggaa
    12061 ttcaaggaat ttttccctaa caatgctgtt gaatattttg tttcttatta tgattattat
    12121 cagcctgaag cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat
    12181 gatgagatcg acaaattacg acattcggct actagttcat tattagaaag aaatgatgtg
    12241 atcgtcattg catccgtttc atgtattttt ggattaggtt cgccttttga gtaccaaaaa
    12301 caagtagttt cgattcgaca gggagcagaa ctagatcgca atcagttgat tcgtgactta
    12361 gtcagtatcc aattcgaaag aaatgatata gattttcagc gcggtcgttt tcgagtgcgc
    12421 ggtgatgtcg tggagatttt tccggcttca agagatgaac gtgccttacg tgtggagttt
    12481 tttggagatg aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga agttttagga
    12541 gaaacggaac acgtggcaat cttcccggcg actcactttg tgacgaatga tgaacatatg
    12601 gagcatgccg tagcgaatat caaagcggaa ttagaacagc gtttaactgt cttgcgaaat
    12661 gaaaacaaac ttctggaagc tcaacgttta gaacagcgaa ccaattacga tattgaaatg
    12721 atgttagaaa tgggctacac gtcgggaatt gaaaattatt ctcggcatat ggacggacgt
    12781 aaagaaggtg aaccgcctta tacgctattg gatttctttc ctgaagattt tttgatcgtg
    12841 gcggacgaat cgcacgtaac catgcctcaa gtcagaggaa tgtataatgg tgaccgagcg
    12901 agaaaacaga tgctagtaga ttacggtttc cgtttgccat ctgctttaga caatcggcca
    12961 cttcggttag aagagtttga aaaacatgtc aatcagatca tttatgtctc tgcgacgcca
    13021 ggaccatatg aacatgaaca aacagatacc gttatccaac aaatcatccg tccgacaggg
    13081 ctgcttgacc cagtgatcga agttcgtcca atcatgggac aaatcgatga tctagtaggc
    13141 gaaataaatg aacgagtcga aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa
    13201 atggcagaag acctcacaga ttatttcaaa gaattaggga tcaaagtcaa atatttgcat
    13261 agtgatatca aaacgctgga acgaacagaa atcattcgag atcttcgatt aggtgaattt
    13321 gatgtgttag ttggaatcaa tttgcttcgt gaaggattag acgtacctga agtttccctt
    13381 gttgctatct tggatgcgga taaagaaggc tttttacgta gtgaacgttc cttagtccaa
    13441 acgattggac gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta
    13501 acagattcta tgcgtctagc gatggatgaa acatcaagac gccgtacgat tcagcagaag
    13561 tacaatgaag aacatgggat cgttccaaaa acgatcatta aagaaatccg agatttgatt
    13621 tctattacga aagaatcgga agatgataca aaagaagcgg tacaagtctc gtatgaagaa
    13681 atgacgaaag aagaaaaaga tacgttattg atgaaattag aaaaagaaat gaaaaatgcg
    13741 gcgaaagcat tggattttga aacagccgct aatgtgcgag atatgatttt agaattaaaa
    13801 gcttcaaagt aggaggacaa catggctaac gataaaatta cgattcacgg tgcacgtgcc
    13861 cataacttga aaaatataga tgtaacgatt ccacgagata aattcgtggt cgttacaggt
    13921 ttgtccggtt ctggaaaaag ttccctagca tttgatacgc tgtacgctga agggcagcgg
    13981 agatacgtag agagcctttc tgcttatgct cgtcagtttt taggacaaat ggataagccg
    14041 gacgtagata gcatcgacgg attgagtccg gcgatttcta ttgatcagaa aacaacaagt
    14101 aaaaatcctc ggtcgacagt aggtactgtg acggaaatca atgactattt acgcttgttg
    14161 tttgcacgtg taggacatcc gatttgtccc aacgatcata tcgagattac cagtcaatca
    14221 gtagatcaaa tggcagatca agtaatggct ttgcctgagc gcacaaagat ccaagtgttg
    14281 gcaccgatcg tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa
    14341 gagggatacg tccgcgttcg agtcgatggt gagatctatg atttaagtga agtgcctgag
    14401 ctggagaaaa acaaaaaaca tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa
    14461 gggattcgtt ctcgtctatt tgattcttta gaggcggctc tgcgattggc agacggctat
    14521 gctctggttg acgtgatcgg tgaagaagaa atgttgttta gcgaacacta tgcctgtcct
    14581 tattgcggat ttactgttgg cgaactagaa ccaagactgt tctcattcaa tgcgcctttt
    14641 ggagcttgtc cagactgtga tggtttaggt gtcaaattag aagtcgatca agatttagtg
    14701 attccagata gaacgaagac acttagtcaa ggtgctatcg ttccatggaa tccaatcagc
    14761 tctcagtatt atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat cgatttagat
    14821 acaccattcg aagaattacc ggaagagcat caggatatca tcttgaatgg gacagatgat
    14881 cttttccatt tccattatga aaatgatttt ggtggcgttc gcgatgtaga aacgactttc
    14941 gaaggggtat tgaagaatat cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt
    15001 gagcaaatgc gtctttatat gacagaatta acttgtcaga catgtaaagg ttatcgattg
    15061 aatccccaag ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg
    15121 tctattcaaa aagcagtaga tttcttctca aacttaacgt tatctgaaca agagaaagta
    15181 attgccaaac cgattttaaa agaagtaaat gacagacttt cattcttgga aaatgtggga
    15241 ctagactatc taactttgag tcgcgcttcc ggaacattat ctgggggaga agcgcagcga
    15301 atcagattgg ctacacaaat tggatcgaat ctttctggag ttttgtatat tttagatgaa
    15361 ccatcgatcg gattgcatca acgcgacaac gatcgactga ttggctcatt aaagaaaatg
    15421 cgtgatcttg gcaacaccct cgttgtagtc gaacatgatg aagatacgat gtgggcagca
    15481 gattatctga ttgacgtcgg acctggggcc ggagaacaag gcggacaaat cgtggctgca
    15541 ggcactcctg aagaagtagc taatgatgaa aattctttga cagggaaata tctttcaggg
    15601 aaaaaatcaa tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc
    15661 actggtgctt cagaaaacaa tctgaaaaat attagtgttg aatttccatt aggagaattt
    15721 gtagcagtca caggtgtgtc tggttctgga aagagtacat tggtcaatag tatcttaaag
    15781 aaatcgttag cgcaaaaatt aaataagaat tctgctaagc caggtaaatt caagacaatt
    15841 tccggctacg aaagtatcga aaagatcatc gatatcgatc aaagcccaat cggccggacg
    15901 ccgagaagta atccagcgac ttatacaagt gtatttgatg atatccgtgg gttatttgct
    15961 caaacgaacg aggcaaaaat gcggggttat aagaaagggc gttttagttt caacgtaaaa
    16021 ggcggtcgtt gtgaagcttg tcgcggggat ggaattatta agatcgaaat gcactttttg
    16081 cctgatgtct atgttccttg tgaagtatgt catggcaaac gatataactc tgaaacatta
    16141 gaagtgcatt acaaaggaaa aagcattgct gatattttgg aaatgacagt agaagatgct
    16201 gtagaattct tcaagcacat tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt
    16261 ggcttaggtt atgtgacgat ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa
    16321 cggatgaaac ttgccagtga attgcacaaa atctctaatg gaaagaattt ctatatacta
    16381 gatgaaccaa cgacaggact tcatagcgat gacatcgccc gcttgttgca tgtattacaa
    16441 agattagtag atgctggtaa cacagtttta gtgattgaac acaatctaga tgtaatcaaa
    16501 acagcagatt atatcattga tttaggacca gaaggtggag aaggtggagg aacgatcctt
    16561 gcgactggaa caccagaaga aatcattaac gtaaaagaaa gttatacagg tcactatttg
    16621 aaaaaaataa tggtataaag aggatagata gctgagatag ttaaaaaaac ataaaaaaat
    16681 gaatttttgc ttgttttatt aatatcagtg ttctagaatc attacatggt agttctttct
    16741 acactgattg gtatagacaa aaacttccta taagttcaat ccgttcaaaa aagaattgag
    16801 caatcggagg agtttccttt gattgctttt ttctttatgg tatgatattc cttgtgtagt
    16861 tggaaaagaa aaacaagaga ttatgagtaa gtgagagata atgatgaaca gtattgaaaa
    16921 tgaagaattt atccctatat tattaggaag cgacatgaac gtttatggta tggcacgttc
    16981 ttttaatgaa gcatacggca aaatctgcca agcatatgct tcggatcagt tagcccctac
    17041 ccgctacagt aaaattgtca atgtagaagt tattccagga tttgataaag accctgtgtt
    17101 tatcgagacg atgcttcgtt tggcaaaaga aagatattct gataagagta aaaaatatct
    17161 tttgattgct tgtggtgatg gatatgcaga attgatctcc caacataaac aagagctgtc
    17221 agaatatttt atctgtccat atattgatta ttctttattt gaaagactga tcaataaagt
    17281 aagcttttat gaagtttgtg aagaatatga tcttccgtat cctaaaacat tgatcgtcag
    17341 agaagaaatg ttagtaaatg gacatttgga acaggaattg ccttttgaat tcccagttgc
    17401 tttgaaacct gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc
    17461 ttttatttta gagactagag aagagttcga tttaatttta ggccggatat acgaagccgg
    17521 ttataaaagt gaaatgattg tacaagattt tatcccgggt gatgatagta atatgcgtgt
    17581 gctaaatgcg tatgtagatg aagatcatca agttcggatg atgtgtcttg gtcatccact
    17641 gttagaagat cctacaccag catcgattgg taattacgtt gtgatcatgc cagattataa
    17701 cgaaaaaatc tatcaaacca ttaaggcatt tttggaaaaa atcgaatata caggctttgc
    17761 caatttcgat atgaagtatg atcctagaga tggagaatat aaattatttg aaatcaattt
    17821 gagacaaggt cgtagcagtt tctttgtgac attgaacggc ttgaatctgg ctcgtttcgt
    17881 cacagaagat cgagtgttta acaaaccctt tgttgaaacg acctatggaa cgaatcaatc
    17941 agataaagca agattatgga tgggtgtgcc caaaaaaatc tttttagaat atgctcggga
    18001 aaatgaagac aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg gtactactgt
    18061 gttttatgaa aaagaccggt caatcaagcg ctggttgttg atgaaatata tgttccataa
    18121 ctatattcca agattcaaaa agtattttca tgtgaaagaa ggctagttca tggagaattt
    18181 tttcagtatt ttaggcggaa tgggcacgat ggcgacagaa agttttgttc gcttgatcaa
    18241 ccaccgaacg aaagctacaa aagatcaaga atatttgaat tatgtcttat tcaatcatgc
    18301 aacagttcct gatcgtacag cttatatttt agatcgatca gaagaaaatc caatgccgtt
    18361 tctgctggat gatattgaga aacaaaattt attgcagccg aacttcattg tgttgacttg
    18421 taacaccgca cactactttt ttgaagaatt acaagcggca acggatattc ctatccttca
    18481 tatgccaaga gaagcagcaa atgaacttgt ccgtcagcat acgacaggga gagtagcgat
    18541 tctaggaaca gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg
    18601 attcgaaaca gtgattcctg atactgttct tcaagaaaaa atcaattatc tgatctatca
    18661 tgagatcaag gaatcggatt atttgaatca agagctatat tacgaaatct tagaagaagc
    18721 ggtagagcgc ctaaactgtg aaaaagttat cttaggttgc acagaattgt cattgatgca
    18781 tgaatttgct gaagataacc attatccagt gatcgatgcc caatctattt tagcagatcg
    18841 gacgattgaa cgagcgctag ctgaaagaag caaggcattg gatacagcat cggaaaagta
    18901 aaaaaattgg atttggggca aatatcctaa aagatatttg gtccaaatct ttttgtaaaa
    18961 gaaaaagaaa actggtaatg atacgcccgg aatgatataa ttttactatg cacaatgtga
    19021 gaggagttcg taattatgac agataatttg caattagtca tcataacagg tatgagcggt
    19081 gcagggaaaa cagtagctat ccaaagtttt gaagatatgg gctatttttg tatcgataat
    19141 atgccgcctc gattgattcc aaaattctgg gaattaatca aagaatcagg caaagtcaca
    19201 aaaatggctt taggagtgga tttacgttct cgttccttct ttgaagaaat ccaaaacatg
    19261 ctgatcgaaa tcgaaaatac gaacttcatc gacacttcta tcttattttt agatgcttca
    19321 gacgaagaac tagtggctcg ctacaaagaa acaagacgag cacatccaat ggcgatggac
    19381 ggtttgatca ctgaagggat acgtaaagaa cgggcgatac tagacgactt gaaagcccaa
    19441 gcttccgtga tcattgatac gacgacattg acaccacgac aattacgtga aaaaatcaat
    19501 gagtcattca aatcaaaggg agaccaagga tttcggatcg aatttgtatc atttggtttt
    19561 aaatacgggt tgccgatcga ttcggatatt gtgatggatg tgcgtttttt gcccaaccct
    19621 cattatatcc cggaactaag accattgacc ggaaaagatc agccagtcta tgattatgtg
    19681 atgtcctttg atgaaacgca atcattctac cataaatttt tggatcttct ggaaactgta
    19741 ttgcctggat acattaaaga aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc
    19801 ggtcaacacc gttctgtggc attgactgaa cgtttggcaa atgctttagg gaaaaactat
    19861 aaagtcaatg tgacacatcg ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga
    19921 aaatgaaaac ctaccggata agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc
    19981 tgccagttat cctgaaaagt ttgcgaaatc aaggagcaga tatcacagca gtagtgactg
    20041 ttgcagatga tgggggaagc agcggtgcaa tcagagaatc gatcgctatg gctcctcctg
    20101 gtgacttgag aaatgtctta gtggcgttat cggatatgcc acaattctat gaagatattt
    20161 tccaataccg tttcaaaaag gaagatcagt ttttagccaa tcatacgatc ggtaatctga
    20221 tcattgccgc agtttccgag atgcggggaa gcacttatga ggcgattcaa ttattagcaa
    20281 aaatgatgca tgtagatgga catgtttatc cttcttcaga aactcctttg accttgcatg
    20341 ctgtctttaa agacggaacg actgcagtag gagaatctaa aatcgcgatc gaccgtaaaa
    20401 ccattgaccg tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa
    20461 aagtcgtttc agcaattatg gacgccgaca tggtcgtttt gggacccgga agtttattca
    20521 ccagtatcct tccaaattta gtgataccag aaatcggcga agcaatgctc aagacaactg
    20581 ctgaaaccgt ctatatctgt aatatcatga cccaaaaagg ggaaacggaa catttcacag
    20641 atgccgatca tatacgtgtt ttacacaagc atttgaatca gccttttatc gatacagtat
    20701 tggtcaatac ggaaaaagtg cctgaagatt atatggaccc agagatctat gatgagtatc
    20761 tagtacaagt cgcacacgac ttcaacggat tgagaaatga aggatgtcga gtgatttcca
    20821 ccgacttttt ggaattgaaa gatggtggtg tcttccacga tggagacaaa gtagtagaag
    20881 aattgttccg tttagttttc ggttcaaaat attaagcaat ttattgttgg agaaaggagg
    20941 agaataggat gtcatttgct gcagatgtga agaaagaact aactggtttg gaagttcatc
    21001 gagaacatgc gaaagcggaa ttagctgctt tgctgcgaat gaacggctct ttaggtattg
    21061 tgaaccagca gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct
    21121 attctctttt gaaagatcat tatcaggtac gaagtgagct actcgttcgt aaaaaaatga
    21181 aattaaaaaa gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga
    21241 tggatcttga tatcatggat ggtatgatgt tcaatggaca cgtttcggac gagattatgg
    21301 gaaattcgca aaaaatgcgt tcttacttac gtggtgcttt tatggcctca ggttcagtca
    21361 ataatccgga aacgagccgt tatcatttgg aaatattttc tatttatgaa cagcataacc
    21421 aagacatttg cgatatgtta aattattacg gtctacatgc tcgaacgctt gaacggcgca
    21481 atggctatat ttcttattta aaaggagcag aaaaaattgc tgatttctta acattgattg
    21541 gtgcaaccaa ctcaatgctc cgatttgaag atgttcgaat cgtacgggat atgagaaatt
    21601 cggtcaatcg tctagtcaat tgtgagacag caaatctgaa caagacgatt gatgcggctt
    21661 ctaaacaaat cgaaaacatc gaattgatcg aagcaaaggt cggtctacat gcccttcctg
    21721 aaaaacttca ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat
    21781 taggggaaat gattccctca ggtgcgatat caaaatcggg aattaatcac cgaattcgca
    21841 aaataaatga atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg
    21901 atacatgaag caggagatgc gaatgtatca gtttttttta aaagttatac ttgcttttct
    21961 tccgtcaaac tataattcca tctttactgg agtaacataa tttggaaagg cttctattgt
    22021 ttttaataca atcgtataaa aagcggggag acaactgaat gaagatttta gttttgtaca
    22081 taaatttttg cttaatatag aagtcctgat atagataaga tcaagtattt atctataact
    22141 ggcctgatta attaaaaaac tggaaggaac tgctcgatca tccttgagaa ctgctcttct
    22201 tacttctggt caagattctc gtggttggtg gataaggatg gttgataagt tcattggatt
    22261 agctagtcga aaacaaccag gtgtaattaa tgtagaagtt ttaacaaggg gatggagtga
    22321 tttattcagt gataatcata agttcataaa tagtaattga gatgtatggt tggaatacgt
    22381 tatacctgaa cttgaactac caagatttgg tttagaattt gagaatcgat gtttataaag
    22441 ggagggcaaa aatgcgtttt tttacaactt ttactatcat tatgattgca gtacttttta
    22501 tttttctaga tatagcaaag cgaaatacag catttttatt atatcgggtt ttacttcgag
    22561 caggtctcat aacatttata tcgatcgttg gattttttct attcacagtt attgtattta
    22621 tttggagaac tccagcacca ccattgccag agattacata tggagaattt ccttttagac
    22681 ttgagtatga attgaatgaa gaattacatg tgatagaaga cacgttaata gttgaatttg
    22741 atggatttgg aatgaacgaa ggcataggga gatatcgtag atggacaagt cgtttagcca
    22801 gcggagaaga tttagtgttg ctgttagaag ttagtgataa taagcaaata ttttattttc
    22861 caggacctgc taattattac atgggagata ggttgaatgg ttataatcat acttttccta
    22921 gtgcttcttt cattgaaagg gaaagaggca ttactagaag ggatatttta catgacaaag
    22981 aattattaga acaatttggt ccgcttgatc aaaatacaat aaatgaagaa gaattgttaa
    23041 atcaatataa tataaggcta gtgaattggg aaatatctga accgattgtt aacaattttg
    23101 gtgattagaa aacaattacg ataaatagaa acaagccaaa tcatcagcaa gtaaaagctg
    23161 atgatttggc ctttcttata cgagtagtag gaattcatag cgaatattat tacgatcagt
    23221 agatattttg atattaaagg agtattacga actagtatgt gagaatgtag aggtgatttg
    23281 cgaataatga gaaatttatc aaatagaaac aaaatattaa tcattattgt tgttatagct
    23341 gtgtttcatc tgggaacgaa tgcggtttta agtagaataa tattgggtcc taagccacca
    23401 agacctgcga ttacccgagg ggagtttgat tttagattag aatatgaagt agatggggaa
    23461 aggattgtga tagaagatac aattgttgca ttatttgatg gattttctgc agatgcaggt
    23521 agcatggcat ggtatcgcac gtggcgtttg catttggcga gtgacaagag ggcacaacaa
    23581 ttattgttac atgagttaga tgatggtcga aaaatatatt atcattttgg aggagctgga
    23641 ttttatatgg ggaagttagt agcagtgtaa ctgtaacaag gaaagatttg ccaggtcttg
    23701 gcattcgaaa ctccatattt tatatcgagc gtgggagaga tacatctatt gggaacagga
    23761 aagggttaac tcttgaagag ttatataatg actttggaat ccgtcttgtt agttgggaac
    23821 acaatccacc aatagaaaat cgctttgaat aatcgaaatc tattaatcca tttcatcagc
    23881 cagtgaatgt tgtaatggaa caatttggtt tagtgttcaa aaccattgaa atactaggct
    23941 gtgatcagca aaaatgtatc tgaataaaaa agagagcaac tattaaatga gaggagttag
    24001 gagttgttag ctttatacga atcaaagtgg caaatacaga tgcgtatgca tttgtgtttg
    24061 ccatcttgcg tttggtagac gagataggta taagctaaag aaacaacttg tttaaaataa
    24121 acgacctagc tcaacaaacg accatctaat caactaacag catacataaa agcaactatt
    24181 tttgaagtag aattgaaatc gggataactt aaaaagtgat tgggatacta gtgtgaatca
    24241 ccgtacataa atattactcg ttaagggaat ttgagaaatt ttgtatgaaa atttaagaat
    24301 tagttagtat gcgcaagtca aaaatatgaa taaattattt ggtggagata atttgctcag
    24361 cttgtcataa gaaatgagat aatctggcag atgcaagctt ttggacacca tttattcgga
    24421 ataaggaggc tgggacagaa gcgtttaact ccaagaaata agaaggagtt gcttctgctt
    24481 ccgccgttta taggtgttta gagcgtgaga caaaagtgtt ctttactttt gtcctacgct
    24541 ccttattttt ttattttgtt tggatcaatt cttttaattc ctctattgaa atagattcat
    24601 caaagatttc tttacccaag atgacagtcg gaacaaatcg aatatgtgct tgctcagctt
    24661 cgttgatgac agcttggctc agctgttcgt ctttttgttc tgtcagcttt agtttatcta
    24721 ctgcaaaatc cgctacttct tggagagaaa gatgtttcca ctcgtcttga gtatcgaata
    24781 tttgtttgat ctctttgatc gctttttgac catcgctgat tgtgagatac cggtgcatca
    24841 cgtttcctcg ctgcaagctt tctttttctt tatcgaacaa tttgatcact cgctgcaatt
    24901 gcccgctttg gactgcttct tctaatagct cataagattc ttcaaaccat tgtcggcaat
    24961 acggacaaga taggttgatg aattcgacca tttttttagg agcattcgat gaaccataaa
    25021 ggattccttt ttgtgtattt gttttcgttg catcaatcac tgatatatcc atgttaaaac
    25081 acctctttcg cttatagttt atacatgtct gacaaaaaaa ggcaacgatt tagactgttt
    25141 gtctaaaaat tagatgacca attcaaatag acatatgtga aatattttta taaataaaag
    25201 aaaaaatgga atgttttacg ttataatgga acagataata agagagaata tgaacaagga
    25261 tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata cggcagccgc atatatttct
    25321 tatgcattta ccgagattgc tgcaatatat ccaataacac ccagttcgac catggctgaa
    25381 ttagtagatc aatggtcagc agcaggaaaa gaaaatgtat tcggtcaacc agtaaaagta
    25441 gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc atggatcatt gaaaactggt
    25501 gcactgacaa ctacatatac cgcttctcaa gggctgcttt tgatgatacc gaatatgtac
    25561 aagattgctg gggaactact gccaagtgtt ttccatgttg ctagtcgggc agtgacgaca
    25621 aatgcgttga atattttcgg agatcacgga gacgtgatgg ctgcaagaca aacaggattt
    25681 gctatgttag ctgaaagcag tgtacaagaa gtgatggatt tagcacctgt tgcccatctt
    25741 gcttctattg aaggagaagt accttttttg aatttctttg atggattccg tacgagtcac
    25801 gagattcaaa aaatcgaagt tttggaatat gaagaattag ctcctctgat caatcaggag
    25861 aaattagctt catttcgtaa acgagggatg aatcccaatc atccgactgt tagtggtacg
    25921 aaccagaatc cagacatcca ttttcaacaa cgggaaacga tcaaccccta ttatgataaa
    25981 ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc gattacgagg aacttcttat
    26041 gacttggtga cttattacgg agcagacgat gcggaagaag tcattgtttc tatgggatca
    26101 gttgcacaga cgattgaaca aacgattgat tacttgaatg caaacggacg gaaaacaggt
    26161 tttttgaata tccatcttta tcgtcctttt ccagtggaaa cattcgtgca aaagttgcct
    26221 catacagtaa aaacaattgc tgttcttgat cgaagtaaag aaccaggggc aggtggagaa
    26281 cctctcttat tagacgttca aagtgcgtta tatgattctg aacttcgccc ggctgttatt
    26341 ggtggtcgct acggattggg gtctaaagat gtgactcccg accaaatctc agcagttttt
    26401 gatgaattga aaaaagaccc aagcatacgc aaaaaacggt ttaccatagg gatcgtggat
    26461 gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg atttgacaga accgcaaaca
    26521 ttccaggcca agttttgggg atttggatct gacggaacag ttggagcaaa taaatcagct
    26581 atcaagatta ttggagatca caccgataaa tatgcgcaag gctattttta ttatgattcg
    26641 aaaaaatctg gcggtctgac tgtctctcat cttcgttttg gagatacgcc gatacgttca
    26701 gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca caccagctta cctgcatagc
    26761 tatgacttgg tcaaaggatt gaagccaggt ggcatttttt tattgaatac tctttggagc
    26821 gacgaacaat tagaaacgca tctgccctta aaattaaaac gttacttggc tgaaaataac
    26881 attagattct atacgatcaa tgccatgaga ttggcccaag aagtcggttt gggacgccgg
    26941 atcaatacgg caatggaaac ggcttttttt aaattagctg acatcattcc ttttgatgag
    27001 gtgcttccgc tgctaaaaga agaagccctg aaaagctatg gacataaatc aatggctatc
    27061 gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac tgcttcatca agtcgcgatc
    27121 ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca ataaaaggaa cactggttat
    27181 atacaagaaa tacttgaacc agtgaatgca caggaaggaa atcaactctc tgttggtaca
    27241 cttgtagcaa atggaatgac aactggcgaa atgcctttag gaacggcttc tatggaaaag
    27301 cggggcgtcg ctttagaagt acctgagtgg attagtgatc gctgtacgat gtgtaatgaa
    27361 tgtgcctttg tttgtcctca tgctgccatc cgtccatttt tagcagatga agaggaaatg
    27421 gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag gcgcagacgg cgtaaaatac
    27481 cgtatccaag tttcagtgga agactgtaca ggttgtggtc tgtgtgtaga ggcttgtccc
    27541 gcaaaagaaa aagctttagt gatgaagcct tatgaagaac agaaagaaca agctgtgaat
    27601 tgggcttttg caatgacatt aagacaaaaa gcaaatccag tcaaaggaaa gaatactgtc
    27661 ttgtctacac aattcaatca accgcttctt gaattctcgg gcgcttgctc aggctgtggc
    27721 gaaacacctt atgtaaaatt attgacacaa atgttcggtg atcgaatgat gatcgcgaat
    27781 gcgacaggct gttcttctat ttggggcggt gcttctcctg cttctcctta tacgaccaat
    27841 gaatgtggtc aagggcctgc atggtcaaat tctttattgg aagataatgc tgaatttggt
    27901 tatggtatgc ttctagcggc acagactcgt cgcgaggctt tggcaagttc aatcaaagaa
    27961 gtattgccaa aagtatcaga agaactacga gaattgatgg aagactggtt ggtacacatg
    28021 tttgagagcg aaggaacgca gcaaagagca gcaaaactgc aagctgcgat cgaaagcgaa
    28081 cgatcaattg tccctgaact ggaggatata tacaagagaa aggatctatt cgtaaaaaac
    28141 agccaatgga tgattggtgg agacggatgg gcttacgaca ttggttttgg cgggatcgat
    28201 catgtacttg ccagcggagc agacgtgaat attcttgttt tggataacga agtctattcc
    28261 aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg caatcgctaa attttcggct
    28321 agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg cgatgacata tggaaatgtt
    28381 tatgtggcgc aaattgcttc aggtgccaat caaatgcaga cgataaaggc ttttgaagaa
    28441 gcagaaaagt atcctggacc ttctttaatc attgcctata cgccttgtat cacacatggt
    28501 ttgcttggtg gaatgaaaga atcgcttaaa gaagcaaaag aagctgtttc ttctggctat
    28561 tggtcattat atcgatataa ccctgcaatg gtcgaaaaaa acaagaaccc aatgactttg
    28621 gactacaaaa aacctgattt ctctgctatg cctgatttca tgaggaaaca agttcgcttt
    28681 tcctcgctag agaacgcaaa tccaaatctt gcaaaagctt tatttgaaaa gactatccaa
    28741 gacgctaaaa cacgttttta caactatgct gcactgacgg gagaattaga aaaaatcaag
    28801 aaacgacttg aaccagagga atctatcgat gtacccgtta agactagaaa agaaaaaacg
    28861 acggaccttg aagcagaaga aagaagagct gccagagcag aacgacgtaa aaaaatggaa
    28921 caagctacaa aagaaaaaaa ggaaaaataa cagaaaaaat ataatgaata attctctgaa
    28981 gtttaaaaat atttattgca gagaaaaata aaacgcatac ttgcaatttt cctgaaaatt
    29041 atgcgttttt ctcaagaaaa atacgagcag atcagcctct aaaattaaaa catatgatat
    29101 actagagagg aaatctcaaa agagaagaga ggctttttat gtcatttcaa atcggggaac
    29161 tttttaattt ggactattgg cagcaaatgt tttccggtga cttgttctct ctgaattttt
    29221 tggtgaacat acttgatatt ctggttgtct ggtatcttgt atacaaactg atccaactgc
    29281 tgcgaggcac aaaagcgatt cagcttctga aaggtgtagc tatttttatt gttatccgaa
    29341 tcgtagcgga aatcattggt ctgcacacct tgtcttggct gatgaaccaa gtcattacgt
    29401 acggggtgat agctgctatc gtgatttttc agccggaaat ccggagagga ctggaacatc
    29461 ttggacgcag ttcgcttttc aaacaatcaa aaagcgaaca gcaggaagat gagaagatga
    29521 tcttgtcttt tgacaaagcc atccaatata tgtccaagcg aaaaatcggt gcgctaatta
    29581 ctatcgaacg gcatactgga ttagatgagt atattgagac aggaattgca ttagatgcag
    29641 atatcactgg agagctgctg atcaatatct ttattcctaa tacaccgctg catgacggtg
    29701 cagttattgt aaaagaagga aagatcgcag tagcaagcgc ctatcttcct ctatcagaaa
    29761 gtatgttgat tccaaaagaa ttcgggactc gacaccgggc agctgtcggt ataagcgaag
    29821 tcagcgacgc gattaccatc gttgtctctg aagaaactgg ggatgtcagc attacattag
    29881 ataatgaact gatggctggc ttatcccagc aagaatattt ggcaattttg cgtagagaac
    29941 tgattgctga agagaaaaca aaaaacaaga aaaacgtgat tcaatctttc ctagacggta
    30001 tgactaaggg aggggatcgc aaatgatctc aaaagagaaa cggacaagca ttctttacag
    30061 ttttattgca ttgctgttca gtattgcgct gtacttcaat gcaaatggcc agtccgttcc
    30121 aaatacgctt tctggaaatg aagcgtataa tcaaacagtt actggggtgc caatcaagat
    30181 ttcttatgat tcaaagcgct actatatcca tggatatgaa aatgcagtga ctgtcaaact
    30241 ttccagtgca aaccgagttc agttgaatgc agaggcaaac gaagatacac ggatgttccg
    30301 cgtcacagca gatatcacta agctgggcga aggaacacat gaggttccgc taaaaattca
    30361 aaatttgagc agtgctgtca cagcaaagat cgaaccatcg actattacag taacggttga
    30421 gaaaaaggta ataaaagatt tcgatgtgga aacgttgatt ccttcaacgt tcacaccatc
    30481 tggatatgaa cttgatgata cttccgttag tcctaaaaaa gtctctatca ctacaggtga
    30541 caaaacattg gcagaaatca aacgagtagt agctgatgtg gacgcttcga tggtcacaga
    30601 tgatgggatt aatagtgagg tcccaataca ggcgctgaat gcagcagggg aagtattaag
    30661 tatcgtctct gatccagtgc aagtaacagt gagagctgat tcgatcaagc caagcaagtc
    30721 ggtacgactt tatgggatac agcaaggaac accagctgcc ggggtagaaa gctatgattt
    30781 cagtttttca aaactggaag cagaagtaag cggctctagc gatttgcttg catcgatcgg
    30841 tgacagtatt gctgtaccga tcaatgtctc aggtatcaat catcgaacaa ctcgtaaaat
    30901 tgaaatacca gtagaagagg ggttaagtat caagcccgaa tcagttagtg tggagattac
    30961 acccgtgttg caaacgtcaa gcagtagctc tgcttcgacg agcagtcgga cacaagaaaa
    31021 tacaagtacg agtagaagta tttcatctgc tgattcagga acggaaccat cgacaacaac
    31081 catttcttcc agttcggaat ctgggacaag cagctcgacc acaagtacag aagaaacatc
    31141 agaaacaagc gaatcaacac aaatgagcag aacaaattcc tcagaaaatt aagttaaagg
    31201 agtaatttta caactatggg taaatatttt ggaacagacg gcgtacgcgg tgaagccaac
    31261 aaggaactta cgccagaact tgcatttaag ctgggacggt gcggaggtta tgtcctaagc
    31321 cagcatgaag aagaaggaaa acgcccacgc gtgttagtag ggcgggatac aagaatctca
    31381 ggtcaaatgt tagaagaagc gctgatttca ggacttttat cggtgggtat cgaagtgttc
    31441 cagctaggtg tgatctctac accaggtgtt gcttatctga cacgtttgca aaaagcaagc
    31501 gcaggggtca tgatttctgc ttctcataat ccagcacaag ataatggaat caaatttttc
    31561 ggtgcagatg gttttaaact agtagatgac caagagctag aaattgaagc attattagat
    31621 gctgaagtgg atgaattgcc tcgtccatca gctgaaggat tgggaactgt agaagaattt
    31681 ccagaaggct tattgaaata ttctcaattc cttgtacaaa cgatcaacgg tgacctttcc
    31741 ggtttgacca tttgtgtcga tgcagcaaac ggagcaactt caacaagtgt taatcgtttg
    31801 tttgccgatc ttgaaacaga tttttatacc atgggcacaa atccagacgg gttaaatatc
    31861 aatgatggcg taggctctac ccatccagaa cgtttagctg agatggtcgt tgaaaaagga
    31921 gcagatgctg gactagcttt cgatggcgat ggcgatcgta ttattgcggt ggatgaactt
    31981 ggaaacattg tcgacggtga taaaatcatg tttatctgtg ctaaatactt ggcagaaaaa
    32041 aatcgattga aaaaagatac gatcgttacg actgtcatga gtaacttagg attccacaaa
    32101 gcggtggaag gaatcggttt gaaagatgtc gttacacaag ttggtgatcg ttacgttgta
    32161 gaagaaatgc gcaaaaacga ttataacttt ggcggcgagc agtctgggca catgattttc
    32221 ttagatttta acacaactgg agatgggatg ctttcaggaa ttcagttact gaatatcatg
    32281 aaacagacag gtaagaaatt atcagagctt gcagcagagg taacaatcta tccgcaaaaa
    32341 ttagtgaata tccgagtaag tgataaaaac ggtgcgatgg atgttccggc aatcaaaaaa
    32401 gtgattgaag aggcagaagc tgaaatggga tcagaaggaa gaatcctagt tcgtccttcc
    32461 ggtacagaac cattacttcg agtgatggca gaagcgccta ctgatgagaa agttaattac
    32521 tatgtcgata aaatcgcggc agtagtcaaa gaagaaatcg gactggatta agagatattt
    32581 tcataaaaaa taaaagaaca aggaccagtg tcagatgtct gacactggtc cttgttcttt
    32641 tattattcta ctgtcactga tttcgctaaa ttacgtggtt tgtcaacgtc gaatccccgt
    32701 aatagcgttg cgtaatatgc aagtaattga gtcggtacga ctgtggttag agtagttaga
    32761 taagggtgga cagttggtag caccagctgg tcactttcct tttctaatcc ctctgagaca
    32821 ataactagtg ttttagcgcc acggctttcg acttctttca ggtttcctcg agtatgcgca
    32881 ccagtcactt cttcactgat gattccaatc actggtgttc cttcttcgat caaagcaatc
    32941 gttccatgtt tcaattcgcc agcggcaaaa ccttctgcct ggatataact gatttctttc
    33001 aatttcaaag cagcttcaag agaaacgttg tagtcagcgc cacgtccaat gtaaaaggca
    33061 tttcttgtat cgctgagata tacttctgcc aactcttcta aataatcttt ttgggcgatg
    33121 accgattcca tcgcattagc aactaaactc aattcatgag tgatatcgaa tacaagagaa
    33181 gtttcaaatt cttttttatc accgatagct ttggctaata aagttagtac agctatttgt
    33241 gcagtataag ctttcgttga tgctactgcg atttcgggtc cagcatgcaa tagcagtgta
    33301 aaggatgctt cccgcgaaag agtagaacca gcaacatttg taatcgtaag tgaagggtag
    33361 ccgagacggt tgatttttac cagaacttgg cggctatctg ctgtttcgcc actttgactc
    33421 aagaaaataa agaatggttt ttttgaaaga agaggtgtgt tatagccaaa ttcactggct
    33481 aaatgaacct caacagggat ctgtgtcaat ttttccagag tctgtttacc tgctaacccc
    33541 gcatggtaac tagttccgca agcaacgata tagattcgat cgctatcaga caatgtatcg
    33601 accagctttt gatcaagaga gacacgattg tcttctgtta cgtatttttg agcgatacga
    33661 cgcatgataa taggctgttc gtcgatttct ttcagcatat aaaaaggata ggtacctttc
    33721 tcgatatctg aagaatctac ttgtgcttcg aatgaagaac gttctacgat gtcacctgca
    33781 aaattttgaa taataacttt ttctgatgtg acggtaacca tctcttcatc ttcaatctca
    33841 acgaattcct tcgtatgagc aagcatagcc attgcatcgc tggcaatcac attgaatcca
    33901 tcgccgagtc cgattaatag tggactttta tttttagcga cgtaaataac atcaggtgct
    33961 gtccgatcaa tcaaagcgaa agcgtaagaa cctttgatga cccgcaaagc ttttaaaaac
    34021 gcctcttgcg ttgtagtatc cttcgcgaaa gtttcaatta gatgagcgat aatctcagta
    34081 tcggtctcac caataaagtg gtcatttgct aaataagcaa ttttaagttc ttcaaagttt
    34141 tcgattacgc cattgtgaac gagcacgaag cgtccacttt gggaagtatg tggatgggcg
    34201 ttttcttcag aaggttgccc gtgagttgcc cagcgtgtat gaccaatacc tatatttcct
    34261 gtagtatcaa tatttatttt gtttttcaaa ttttgaattc tgccttgtgc tttgaccaga
    34321 taatcagttg tgccatctga gacaaagata ccagctgaat cataaccacg gtattctaat
    34381 ttttctagac cgtcaatcaa tcctggtgtt acatttttta atccaatcat tccaacaatt
    34441 ccgcacataa aaaacatcct actttctata atgaattcgt atgaatgctt ccagctgcag
    34501 gaaaatcctt ttgttttatc tttgcagata ttttttgtgg attttctgta gagtgcagtc
    34561 cctagggcca tccgccgaat ctttcgataa accctctcct cgtcatctgt ttacacagac
    34621 ctggcgctat cctaaaaggt ataactatcc tcctcttttg ttttttatct ggaaacattt
    34681 aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa ataaattggt
    34741 atatatcaaa tgctttgtca aatgctcttt ttatgtcaaa aaataatggg tttttatcta
    34801 tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg agtaaaaatg
    34861 gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc caaattcgaa
    34921 gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg ggatagcatc
    34981 caagaaatcg cacctgatgc cttggaagtt gctgaagctc gtgagtttac ggtagaagat
    35041 gaattgacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga gcggttcgac
    35101 acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa aggagaaaga
    35161 atgaataaga gacgttgggc agcagttctg attgctgcag gattatttgt ggtttcgtta
    35221 gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt gaacagttgg
    35281 ttatatgggg atgaagaact agcttcaaat gtgttggaag agggagacag tacgcaaaaa
    35341 atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg tttatttgct
    35401 tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga agaagatcca
    35461 acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta cgaaagtgcg
    35521 gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat gtatgtcagc
    35581 atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga taaaatcttt
    35641 gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt gaattattca
    35701 ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc gttgaaagat
    35761 atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca agcatatatc
    35821 gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa atctgaagaa
    35881 gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa agaagtaggt
    35941 ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatgcgaaa ggaacaacag
    36001 cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc tagtacatgg
    36061 ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag tcagatctta
    36121 tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta ttacggaggt
    36181 gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat aagcaacagc
    36241 agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct tttgaaagag
    36301 agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag tatacggaca
    36361 caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca ggttttttta
    36421 tccgaatgtt tgctttcttg gtcgatcttt tgtgtatcgg agctattacg cgtttaacac
    36481 ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg agtatctacg
    36541 gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa ttcaatcatg
    36601 gtcaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat gaagaagaac
    36661 ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta aaatttcctt
    36721 tgctgatgtt agggtatctt ccagcaatct ttaatcagaa aaagcaacat ctaggcgatt
    36781 ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcattcaac caggaagtca
    36841 acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa gtcagatcta
    36901 aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt tttgttttgc
    36961 tactattgaa catgagctga acatgagcaa gaggaggaca ctgcgtgaag ccgattgatt
    37021 ttccagaaaa atatgagaat ttaatgcgag tagcacaaca agcactagca aaccagcaat
    37081 atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc tttgaggcta
    37141 atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca ttgaagcaag
    37201 cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt tattttgact
    37261 tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt acagattttt
    37321 atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa ttatccggac
    37381 aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt ccttcgttgg
    37441 aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat catgagttta
    37501 tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca agggcaaaat
    37561 tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg acgattgaag
    37621 aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag gcaagttacc
    37681 agcaattatg tgtattttca gatcgttacg gtaatgaaga tgcgctgctt agttccgtgt
    37741 taaatgaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat acatacatcg
    37801 aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa acgatgtatg
    37861 aaggtacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt ttgcagcaaa
    37921 tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga aaaacaagga
    37981 ttctatccat tcaatatgtt tacaatttga aagggttagt gtactattta aaggtatgca
    38041 tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg agggaattaa atgactgcaa
    38101 catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt tacgattgcg caagacgaaa
    38161 tccaaaaagg attaacaact gcgtttaata aagtgaaaaa aagcttgaac gtacctggat
    38221 tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat gtacggtgaa gaagctttat
    38281 atgaagatgc attgaatgct gtattaccag aagcttatga agctgctgtc aaagaagctg
    38341 gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag catggaaaaa ggtcaagact
    38401 gggtaatcac tgctgaagta acagtgaaac cagaagtaaa attaggacaa tataaagatc
    38461 tagttgttga aaaacaagac cgtgaagtaa cagatgctga cgtggatgca cgtatcaaac
    38521 gtgaacaaga agcacaagct gaattagtta tcaaagaaga tgctgcagct gaaaatggcg
    38581 atacagtagt aatcgacttc gaaggctttg tagatggcga agcgtttgat ggcggaaaag
    38641 gcgaaaatta ctcacttgaa ttaggttcag gctcattcat tccaggtttt gaagatcaat
    38701 tagttggtca taaatcaggt gaaagtctag atgtaaatgt aactttccca gaagattatc
    38761 aagcagaaga tcttgctgga aaagaagcag tcttcaaagt gacaatccat gaagtaaaag
    38821 caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga tgttgatgac gaagtatcaa
    38881 cattagacga attgaaagaa aaatatcgca aagaattagt agaaacaaaa gaaaaagcag
    38941 cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt tgaaaacgca gaaatcgttg
    39001 aattgccaca cgtgatggtt catgatgaag tacatcgttc aatggacgaa ttcttgaaca
    39061 acatgcaacg tcaaggtatc tctcctgaaa tgtactatca attaactggt tctacagaag
    39121 aagacttgca taaacaattc gaaggtgaag cagaaatgcg cacaagaaca aatcttgtga
    39181 tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga agaagaaatc aatgcagaaa
    39241 tccaagaatt atcagaaaca tacaacatgc caatcgaaca aatcaaacgc gtgttgacag
    39301 aagatatgct ggaacatgat gtgacaatga aaaaagcaat cgaattgatc acaggtacag
    39361 cgacagaagc gaaagaatag aaatcaagta ataaatcatt tctgaagaag ctacttctag
    39421 aaaattgttt attcgaaata agggactgtg acacgatttt gtcacagttc cttatttttt
    39481 gttaagagta aaaaatgcaa aggtataggt cagttgatcc tttgttaaac actttttctt
    39541 tttactaaac tatgttatga tagtaaaaga ctgtaaagtc tttgatagtt tcttgcatac
    39601 tttttgcaag ttaaggggtg aagaccatgt atgacaatcc aagtagcaat gagaccgtac
    39661 gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa gatcgtagct ggtccagggg
    39721 tctacatctg taatgaatgt atcgacctat gtaaagaaat cattgatgaa gaattttatg
    39781 aagaagctgt acgcgaattc actgaagtcc ctaaaccact agaaattctt gaagtattga
    39841 ataactacgt gattggacaa gatcgcgcaa aacgctcgtt agctgttgcc gtatacaatc
    39901 actataaacg tgtcaatcag caagcacaag aaaacacaga tgatgttgaa ctgcaaaaaa
    39961 gcaatatctg cttgatcgga cctacagggt ctggtaaaac atttttagca caaacattag
    40021 caaaaacatt gaatgtacct tttgcaatcg cagatgcgac aagtttgaca gaagctggtt
    40081 atgtggggga agatgtagaa aacattttgc ttaaattgct tcaatctgct gattacaatg
    40141 tggaacgtgc agaaaaaggg atcatctata ttgatgagat cgataaaatt gcgcgtaaaa
    40201 gcgaaaatgt atctattact cgtgacgttt ccggcgaagg tgtccaacaa gccttgctga
    40261 aaattctaga aggaactgtt gcaagcgtgc cacctcaggg aggacgtaaa catccacatc
    40321 aagaatttat tcagattgat acgacgaaca tattgttcat cgtaggtggt gcatttgatg
    40381 gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac gattggattt ggtacacaaa
    40441 accaaaaact ttctgaagac gaaagtgtca tgcagcatat cattcctgaa gatttactaa
    40501 aattcggatt gatccctgag tttatcggac gattacctgt tatggcagct ttagagaaat
    40561 tgacaacaga tgatctggta cggatcttga ctgagccgaa aaatgcgcta gtcaaacaat
    40621 atcaaaaact tctttctcta gataataccg aacttgaatt cgaacctgaa gcgttgagag
    40681 ctattgcgaa aaaagcaatc gaacgcaata ccggtgctcg aggacttcgt tccatcattg
    40741 aagaaatcat gatggatgtg atgtttgata tcccttcaaa tgaaaacatc gaaaaagtga
    40801 ttatcacaaa agaagcagct gaattgtctg gtgagccaac agtaatctat aaagattcag
    40861 agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt cattttggct tttgcgacag
    40921 ccgcttgctt tattcatcac cttgttaagg agagagacca tgaaagtcca tcaagctgaa
    40981 attatcataa gtgcagtggc accaaaacaa tatccagata cacaattgcc agaaatagct
    41041 ctggcaggac gatcaaatgt agggaaatct tcttttatca acacattgat tgatcgaaag
    41101 aatttagccc gtacttccgg taagccagga aagacccaga cattgaattt ttatttgatc
    41161 gagaacgcat tacattttgt cgatgttccg ggatatgggt atgcaaaagt atccaaaact
    41221 gagcgagcaa aatggggaaa gatgatcgag acatatttaa cgactagaga ccagctacgt
    41281 gcggtcgttt ctttagtaga tttgcgtcat aagccttctc aagaagatat ccagatgtat
    41341 caatttttga agtattatga tatccctgta attgttgttg ccactaaggc agataagatc
    41401 cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa aagcgttgga ttttgatcgc
    41461 aatgacacgt ttattctctt ttcttccgtt acaaaacagg gaaaagaaga agcgtgggca
    41521 gcaatcgaac aagcaattga atcataaaaa gaataaaaaa gacttaccag tcaaactctt
    41581 tcataaaagt ttggctggta agtctttttt tgagcttaac ttacttttgt ttcttgtctt
    41641 ttttcttttc attttgtggc tgtggtttta gaaatcgatc gatctcttct tttggtgcaa
    41701 ctgttttttc cggatcgaca gcaaatgatt cgaaaagttt atctaaatta tctgtacgtt
    41761 caaatttcaa ccccattgta aacacctctt tgtttctttt tacttagtct atcatgtaat
    41821 cgtccaaatt ggtttctaag acataaagtt ttagtttttc tttatcgttg gttataatta
    41881 aattataaaa agattttaaa gaggtgagaa aatgattggc gaagataaat tattgatgac
    41941 tatccagctc acagacttaa atgaagaaga aaaaaaacag gaaataagcg attgggctat
    42001 tttgacacgt ctgttgatcc agccaacatt tatccgctct ttcacacaac aagcggatcc
    42061 gtatgatctt cgaaagcttc aagaaaaaat aatgctagca tcggtaagag acgaaagttg
    42121 gttagttgta ggaaatgacg agaacgaatg tagttttcgc ttggaagata atcaattatt
    42181 gataaaaaat gtattgtcta tctctgtttt caaagaaaag caatttttga ttcgagactt
    42241 cattcagaaa aaaatgattg ctcatggggt gttcgcctat atgcgagctt atagcgagtt
    42301 tatctatcac aatacaaaac agatctctca acggctattg tttgagaaaa aggatgaaat
    42361 cgaacattta cctaaaatga aacaacaaaa cggggaagta gtagtcgatt gcaaccaatt
    42421 tccaggatac gatctttttt accaaggttt atgtttcact tcgtgttggg aaatgtatta
    42481 tagtcgctat tatcatcaaa tcatccctaa accgattttt ctcgacatcc aacaagtaga
    42541 aaaagtgaag gaattagaaa acgaagtcat atgtatccag ctttatcgag acccctttaa
    42601 ctggcaaaag ccaaacaatc aaatgtttca atcctatttt agggaccagc ttgggtttga
    42661 tcatcttgct tgggacaatg gtgtcggttt attgaaaccg ccattcgttg aatacattta
    42721 cactgaccat acgatccaat ctgtccagta tcagaatgca caaatgcaac ctgttcctaa
    42781 aaagaatgct tcattttttg taacaaaaag ttatgatatt caacaaggag attataaaga
    42841 aaggcgtgtt cgcggaacac taaatgctca ggcgtatttt ccttgggttg acgagaatcg
    42901 ctcgcgcatg atgtgctata aagttatcga tccgactgtt gctttggata atggaatcga
    42961 agcttattgt tattatatta gggaatatct agaagtagag gtaacagatg aaaaatatca
    43021 agagtatctt ttatccttaa gaatctatgt tccttcagaa tccctgtcag aattaccgtt
    43081 aaaggaaatc aaacatagac tctctgatgt taccttcaaa cgaataaaga aaaagcggcg
    43141 aagtatccaa ttcgatgtca agaaaggtga gcagcatctg cgtgtgcaat ttttagatta
    43201 ccgagaatta gaacaattga ttactttaca aaaaatatga ggtgaagaaa atggataaac
    43261 aactctatta tcgaataatg gttccggcat cattattatc cgtctgtctc ttttttgttc
    43321 ctaattttcc tattcgatca gtcatcggtt ttttggtcat gtttggcggg atgggacttt
    43381 attattatct tcttcataag gaaagaaaac ctgcttctgt ttatatgaaa aaaacaagaa
    43441 gcgaaaataa aaaacgataa aacggcttga tgatagacaa aaaaataaaa aaccagtctt
    43501 tttgactggt tttttatttt tttttgactg atttataatt tttatgaacg tttttgactg
    43561 atttatattg cgagtagaaa aaaaatatgc tattatttgt attgaggtga tcgtgaaaat
    43621 gcttacagag gaacgtagac aaaaaatcct agacttgctt gaacagcaga aaatcgtgaa
    43681 atcacaagat cttgtaaacc tttttaatgc ttctgaatca acgataagac gtgatttaca
    43741 agagctggag aatagtgggt ttctagaaag agttcatggc ggagctaaga aagagcaatt
    43801 attgaatttt gagcagaata tgagtgaaaa atcactcaaa aacgttcacg aaaaacagca
    43861 aatcgccaaa tcggcagctg aaaccattaa tgacgaagat gtcatttatt tagacgctgg
    43921 ctctactacg ttagaaatga ttccttttct gcaaggaaaa cagatcacag tagtgacaaa
    43981 ttccgttcaa catgcggcaa atttagttga tttggagctt gctaccatta ttttaggtgg
    44041 aacgataaag ctggcaacta atgctgtttt aggatctaat gcgatagaac aactaaaaaa
    44101 ttttcatttt aataaagcat ttatgggaat gaacggtgcc catcttgaac aaggattcac
    44161 gactccggac ccagaagagg cagctgttaa acgtttagcc atgaaaaatt ccgaagagac
    44221 atttgtattg attgatcata ccaaattcaa ccagttgtcc ttcacctctg ttgcaccttt
    44281 ggaagcagcc acgatcataa ccgagcgttg tccattagaa ttcgttaatg aattccgtga
    44341 aaagacaaca attaaggagg caatacaatg atttacacag taacacttaa tccatcaatt
    44401 gattatatcg tacgcgtaga tgggttaaaa ctaggcgatt tgaaccgaat gaaggaagac
    44461 ttcaagttac ctggaggaaa agggatcaat gtttcccgta ttttgaaacg gatcgattct
    44521 caatctacag cactagggtt tttaggaggg ttcactggcg aatttatttc gaagtggctt
    44581 gaaaaagaac aaatccagtc tttgttcaca accgtaaaag aagatacaag aatcaatatc
    44641 aaactaaaat cagatactga aactgaaatt aacggtttgg gcccatctat ttcagatgaa
    44701 gaaatcgatg agttgaagca agcattagga acagtaggac aaggagatat cgtcgtattg
    44761 tcaggaagcg cgccggctag cctgcgaaaa ggattttatc aagaactgat tgatatcatt
    44821 cgagaaaaag gtgcagattt cgtgattgat acaacaggtg atgacctaaa aaatgcactt
    44881 gttaagaaac cattgttgat caaaccaaac aatcacgaat tagctgagct ttacaataca
    44941 accttccaat ctgtagaaga aatcattccg tatggtaaaa aattgctagc tgaaggggca
    45001 cagaatgtgc tgatctcaat ggctggagac ggtgctttgc tcttcacgga acaaggaact
    45061 tataaaacga atgtattagt tcgcccattg aaaaattcag taggtgcagg ggattcaatg
    45121 attgccggct ttattggggc ttacagccgt acgaaagatc caatcgaagc ctttaaatgg
    45181 ggagttgctt gcggaagcgc aacagctttt tctgatgact tggcagctcg cgaattgatt
    45241 gatgagttgc tgccagaagt tgaaatcaca aaaattgatt agtttcaagg aggataatca
    45301 tgaatatcaa ggatttaatg gtcaaagaag cgatgatcat ggatttgcaa gcgactgata
    45361 aaaaaggcgc aatcgatgaa atggtccaaa aaatgtttga tgccggccgg atttccgata
    45421 tcgaaacata taaagaagga attctagccc gcgaagcaca aacttctaca ggtcttggtg
    45481 atggaatcgc catgcctcac gcgaaaaaca gtgcagttaa agaagcaaca gttttattcg
    45541 caaaaagcca aaaaggagta gattatgaag ccttagacgg acagccaact tatctgtttt
    45601 tcatgatcgc agctccagaa ggcgcaaacg acacacattt gcaagcatta gcagcactat
    45661 ctcgattgct gattgatgca gatttcgtag gaaaattaaa agaagcaaat acaccagaag
    45721 aagtccaaga cttgttccaa acagctgaag agcaaaaaga agcagaagaa aaagcagaac
    45781 aagaaacaca agctgcagaa gcttctgctc atccagaaag aaaattcgtt gttgccgtta
    45841 cagcttgtcc aacagggatc gctcacactt acatggcaga agatgctctg aagaaaaaag
    45901 cgaaagaaat gggtgtggag atcaaagtcg aaacgaacgg ttcagaagga atcaaaaacc
    45961 gattgactgc tgaagacatc gcccgtgcag aaggtgtaat cgttgcagca gacaaaaaag
    46021 tagagatgaa tcgctttgac ggaaaagaac tgatcaatcg tcctgtcagt gatggtatcc
    46081 gtaaaccaga agagctgatc aatctagccc ttagtggtac tgcaccagta ttccatggtg
    46141 atggaaaaga aagcagttca gaagaaggaa atgcggatgg tactatcgga caaagaattt
    46201 acaaagattt gatgaacggc gtatcacaca tgttaccttt cgttattggt ggaggaatcg
    46261 ctattgcgct ttctttcatg atcgaccaat ttattggcgt gccacatgat caattatcaa
    46321 atctagggaa ttacaatcaa gctgctagct ggttcaacca aatcggacaa gctgcatttg
    46381 gtttcatgct gccggtatta gcaggattta ttgcttcaag tatcggtgac cgccctggtt
    46441 tgatcgttgg atttgctgct ggtgcattag ctaatacagg cggtgcagga ttcttaggtg
    46501 cactgattgg tggattctta gctggttatg tcatcgtatt tttacgtaaa ttattcaaga
    46561 acttaccaaa atcattggat ggaattaaaa cgatcctctt ttatccagtg tttggcttac
    46621 tgattactgg tttcttgatg ttgctggtca atgtccctat gaaagcaatc aatgatgcat
    46681 tgaacggttt cttgacaagt atgagtggtt cgaatgctgc tttacttggt gcattattag
    46741 ctggaatgat ggcggcagat ttaggcggtc caatcaacaa agccgcttat gtgtttggta
    46801 cagcaacttt agctacgact gttgcaactg gcggaagtgt cgtgatggct tctgttatgg
    46861 ctggaggaat ggtacctcca ttggcaattt tcgttgctac acgtttgttt aagaataaat
    46921 tcagcaaaac agatcaagat gctggtttga cgaacatcgt gatgggacta tcgtttgtca
    46981 cggaaggatc gattccattt gcagcagctg acccgattcg tgccattcca agttttatta
    47041 ttggttctgc tttaactggc ggattagtcg gagctttcgg gatcaaatta ctggctcctc
    47101 atggcgggat tttcgttgta ttcttactga gtcacccggt catgtatttg gtgtttattg
    47161 ctatcggagc agtcgtatct ggtgtgattt atggtttatt gagaaaagct cctgcagcag
    47221 cagaagcata gaaataaaaa agaagaggct ggtttttccc agtctcttct ttttttatag
    47281 aatcatatag aatcaagtag tgtagatgaa tagttgttgt aaaatagttt ttccatgttc
    47341 gttaaaatga ttgcttcaaa tttgtgatag aagatttagc ctcgatttcc tgcttgattc
    47401 ttggcaagtt tgcttcatca gtcaagataa ccaaaatatc tcctacattc atgatcgtat
    47461 caccgtgagt caagatttcc tgatttcctc tgcgtataga gattagcagc atatttttag
    47521 gccaattgaa gtctcgtacc attaatccgt ctaaagaact ctctgccgtt actggaaaat
    47581 caaaagttgt ccttgcacct tttagtgagg cggtctgagt atttccaagt agacgttcca
    47641 ataatgattc gtaaattgga ttaccgccga gaaggtcatt gacgatataa gcaacaagag
    47701 cagccaatcc taaaggcatc agatgattaa gagagccaac catttctgtc actaaaataa
    47761 ttgctgtcag cggtgctttt ccaatcgctg tgaaataacc agccatggca ataacgacaa
    47821 aatccttgat gtagattgga tccatcccga aatcaaccat aatgtttcca taaaagctac
    47881 cgattaaagc ccccaatgtc aatatcggaa ggaaaatccc ccctggaaga ccaccaccgt
    47941 aagaaatcat ggaaaaaacg aatcgaagaa caaacaaacc gataatcata atgattggca
    48001 aggaagcatg tccaaaagct aaaacgattt gattgcctcc gcctagagta ctaggccata
    48061 aataaccaat tggcaaaatc agcaagaaag gaacgaaccc gtgaaaataa gctggtaaag
    48121 gcaatcggtt gtaaaatttt ggcaaagcca gcaagacctt ctgataaata aaacctaaga
    48181 tgcctagaag tactcctaag atcactaagg aaccataata tttcaacggc aaagaaggaa
    48241 ggtcacctag atataaaaca ggtctcaagc cgaaaaagta aagagagacg aagttagcag
    48301 tgattgctga agcaaatgat gtcagccaaa caagtggaga aaaggagtga tggatttctt
    48361 ctaaaacaaa gagtaatcct gcgataggag cattgaatgc tgctgcaagt ccgctacttg
    48421 caccgctaga aattaaaatc ttttcttctg aagatggact ccgaaacaat tgtccagctc
    48481 cttgtccaat ggatgcgcca agctgtatag aaggtccttc tctacctaga aaaaggcccg
    48541 caccaactga aagtacaccg ccaatgaact ttttccataa aacagagaac cagttcattg
    48601 ataattcccc gcgaagctgt ccctctactt gtggaatccc gcttcccttg atatccggtt
    48661 catttttgac cagataaccc acgaaaaatg ctgcaaggat gcagcaaata gctaggccaa
    48721 ttaagtaaat cggctgttcc cgactcattt catagatact tcgaactaaa tcgcttagct
    48781 tttcgataga taatcgaaac aggctaacga cgattcctgt cagcagtcca acaaatattc
    48841 cttttagaat aaaatatatc tgagtcttat ctaatttctt gatttttcct tttttttcca
    48901 ttcaaacgcc tcgctttctt ttaatttagc gcgaaatcaa cgaattgaca aggaaaaatc
    48961 cataaaaaga aacaaatgat tttgaactag ctagtttaat gaaagccaag aaaatgctat
    49021 aattaacaag attgataaaa gaaaggatgt tgttaatgtc atccaaagct gaattattaa
    49081 acgggatgaa cccccgtcag aaagaagctg ttcttcacac tgacggacca ttactgctaa
    49141 tggcaggtgc aggaagtgga aagacgagag tgctgacaca ccgcattgcc tatttgattg
    49201 aggaaaaaga agtcaatcct tggaacatcc tagcgattac ttttaccaat aaggctgcaa
    49261 aagaaatgaa agaacgtgtc aatgcgattc tcgcatctgg aggcgaagat gtctgggtat
    49321 cgacttttca ctcgatgtgt gtgcgtattt taagaagaga tgttgatttt atcgggtata
    49381 accggaactt tacaatcatc gattcttctg aacagctgac cttgatgaaa cggatactaa
    49441 aagaactgaa cattgatccg aaaaaatatg atcctcgaag tatattagga acaatctctc
    49501 aagcaaaaaa cagcctgcag acccctcagg attttgcaaa aatgcaaggc agttattacg
    49561 aagagattgc ggcaaaatgt tatgctgctt accaaaaaga gcttcaatat aatcaatgta
    49621 tggactttga cgacctgatc atgaatacga ttcgattatt tgaagaacat cctgattcac
    49681 tcacatatta tcaaaacaaa ttccattata ttcatgtaga tgagtaccaa gatacaaatc
    49741 acgcacagta tacgctagtc aatttattgg caggtagatt taggaacctg tgcgtcgtgg
    49801 gagatgcaga tcaaagtatt tacggctggc gcggtgcaga tatgcagaac atcttggatt
    49861 ttgaaaaaga ttatccagat gctgcagtaa ttttgctaga acaaaactat cgctccacga
    49921 aaaacatcct tagcgcagca aaccaagtca ttgaaaacaa tagcaatcga aaaccaaaaa
    49981 acttgtggac agaaaacaaa gaaggaaaca agatcactta ctaccgtgca gataatgaac
    50041 gagacgaaac ccgttttatc gtagatcgga tgcaagaaga gattcgaagc aatcatcgga
    50101 attacggcga ctttgccatc ttgtaccgga cgaacgcaca atctcgtgta atggaagaaa
    50161 ctctgttgaa agcaaatatt ccttataaaa tggttggagg gcacaagttc tacgatcgta
    50221 aagaaatcaa agatatttta gcgtatttga atgtgctggc aaatcctcaa gattcaatca
    50281 gttttgagcg tatcgttaat tcgcccaaac gaggcatcgg accaggatct atcgaaaaac
    50341 tacgcagttt tgcttctctt catgaatggc cactgcttga agcagcgcaa aatgtggatc
    50401 tggcaaatat cggtggaaaa gcaggacagc aattaggtgc atttggagag atgatccaag
    50461 aagtcactca gatgatccca tacttgacag tgacagaatt gacgaaagag gttttggatc
    50521 gtagcggata tctagaagac ttaaaaatcc aaaatacatt agaagcacaa gcacgcatcg
    50581 aaaacttgga ggaattttta acggtcacac aagaattcga caaacaattc gaacagcaaa
    50641 atgaagaaga cgctgatgca ccagaagaaa aactaacggt cttcttaaat gatctggcac
    50701 ttgtttccga tattgacaat ttagaagaag atgcttccca agtgacattg atgactttgc
    50761 atgcagccaa aggattagaa ttcccagttg tattcctgat tggtctagaa gagggggttt
    50821 ttcctttatc tcgagcattg atggaagaaa gcgaactgga agaagaacgc cgtctcgcat
    50881 atgtggggat cacgcgagca gaagaagcgt tgtatttaac aaatgctttc tccagaacat
    50941 tatatggacg gacgcaatat aaccgacctt ctcgttttgt agaagaaatc gatcaggagt
    51001 tactggaaat cgaaggcatg cgtcctacgc ctaaaaaaac acctgtattc gctaaaaaaa
    51061 cagcatatag ctataaacag ccagagacag cggttgttcc ctctaaaagt gctactggag
    51121 gggaaaataa cagctggaaa cctggagata aagtcaaaca caaaaaatgg ggtctaggaa
    51181 cagtcgtccg agtaagcggg acgtcgaagg atctagaact agatgtggct ttcccaagtc
    51241 aaggagtaaa acgtcttttg gctgcctttg cgccaattga aaaagcttag tagtggaggg
    51301 taaatgatgg aacatgaaat gacgattgac gaagctgcta aacgagcaga agaacttcgt
    51361 acaagactga accaatggtc tcgcgaatac tacgtagaag ataaaccaac agtggaagac
    51421 tatgtttatg ataaagaata cgcagaatta gtagcaattg aggaacaata tcctgatttg
    51481 atcacgtctg attctcccac tcaacgagta ggcggaaaag tattagaagg ttttgaaaaa
    51541 gtcactcatg atattccgct ttacagtttg aacgacgttt tcagcaaaga agagctgata
    51601 gcttttgatc aacgtgtaca aaaagcagtg ggacgtgtag ttgactattg ctgtgaacta
    51661 aaaatagatg gactttccgt ttctttgcgt tatgaagatg gaaatttcgt gagaggtgcg
    51721 actcgcgggg atggaactgt aggagaaaac atcactgaaa acctaaaaac tgtccggtcg
    51781 gtcccaatca aattaaaaga accaatgaac atcgaagtaa gaggcgaatg tttcatgcca
    51841 aaacggtcat ttgtccaatt gaaccaagac agagaagcag agggaaaaga tatctttgcg
    51901 aatcctcgga acgccgcagc tggttctttg cgtcagttag attctaaaat cacagcgaaa
    51961 agaaatctgg acactttttt atacacagta gctgatttcg gtcctatgca agcaaaaacc
    52021 cagtatgatg cgctagaaga gctagaaaaa attggattcc acacgaatcg ggaaaaacgt
    52081 ctatgtcatt cgattgatga agtgtggtca tacattgaag aatatcatga caaacgtgtg
    52141 gatttgccct atgaaatcga tggaatcgtc atcaaagtga atgaattctc gctacaagat
    52201 caacttggtt ttactgtcaa agcaccgaga tgggcagcag cttacaagtt tccgccagaa
    52261 gaagttgaaa cgctaatcga aaacatcgaa tggacagtcg gtcgaacagg ggtagtaacg
    52321 ccaacagcta tcatgacacc agtccgagta gcaggaacga cagtaagccg tgccagcctg
    52381 cataacggtg attatattaa gttgaaggat atccgcttga aagatacagt tctgatttat
    52441 aaagcgggag acatcatccc agaagtttcg caagtcgtgt tagataaacg acctaaagac
    52501 agcgaagaat accaattgcc tacacattgt ccagtttgtg gaagtgaact tgtccatttg
    52561 gatgaagaag tagccttacg gtgcatcaat cctaaatgcc ctgctcaaat gaaagaaggt
    52621 ttgaaccact ttgtttcaag aaatgcgatg aatatcgatg ggttggggcc gcgcgtacta
    52681 gaacagatgt atgataaaaa actagtagca gatgttgctg atctttacaa attgacagaa
    52741 gaagagttat tgacgttgga caaaataaaa gaaaaatcag caaataatat tttgacagca
    52801 atcgacaaca gcaaagataa ttcggtcgag cggttgattt tcgggttagg aatccgacat
    52861 gtcggagcga aagcagcaaa aattttagca gaacatttcg gtgatctaga aacattgagt
    52921 aaaagtgact atgagtcgat tattgcgctt gatacgatcg gtgatatcat tgccgacagt
    52981 gtagtcactt atttcagtaa cgaagaagtc catgaattga tgaatgaatt aaaacaagca
    53041 ggcgtcaatt tcgaatacaa agggcttcga aatgcccaat tgcaagaagt cgagtctcct
    53101 tttaaagaaa aaacagtagt ccttactggt aaactaacac gatttactcg tgaagaagca
    53161 aaagagacga tcgaaaatct tggcgggaaa gtgactggta gcgtatcgaa aaaaacggat
    53221 atcgtggtag caggagaaga tgctggcagc aagctgacca aagcacaaga attaggaatc
    53281 gaagtttgga cagaagatca aatggcagat gcattggcta aaagtcgctc agtagaagaa
    53341 tgaaaaatga gttttttcag acaaatttca ataaacaatt tcagacacgg cactcttgtg
    53401 ttaaaataga gagcaaagtt tttaactcgt ttagaaagaa ggggtagtat ggcgattagt
    53461 gaagaacaag tgaaacacgt tgccaaatta gcaaaacttg cgttcgcaga tgaagaactt
    53521 gcaagtttta ccgaccaatt aggtaaaatc atcgatatgg ttgaaatgct ggaagaagta
    53581 gatacggaag gtgttccatt tacgtcgaat gtggttgaaa cagtaaacgt gatgcgacaa
    53641 gatcgggcgc aagctggatg gaaccgcgat gaattattga aaaatgtgcc ggaaacagaa
    53701 gacggcttta tcaaagtacc cgcaatcatc gataatgggg aggctggcgc ataatgacag
    53761 aattacacaa tcgttctcta gaagaactgc atcatatgct tgtatcgaaa gaaatcactg
    53821 tacaagattt gacaaaaaca acatttgaac gaatcaaaga aacagagcct gaggtcgagg
    53881 cattcattac attgaacgaa gaaaaagcat tggaacaggc aaaagcttta gatgaaaaag
    53941 ggattgatga aagcaacgtc cttgctggta ttccaattgg tatcaaggat aatatcgtaa
    54001 caaaagatat cttgacaacc gctgcatcta agatccttta caattttgaa ccaatttatg
    54061 atgcaacggt catggataaa atctatcaag cagatatgat ccctgttggt aagctaaaca
    54121 tggatgaatt tgctatgggt ggaagtacag aaacttctta tttcaagaaa acaaaaaatg
    54181 cttgggaccc aacaaaagta cctggtggct cttctggtgg ttctgcagca gcagttgcag
    54241 ctggacaaat tccagtttct ttaggtagtg atactggggg aagtatccgc caaccagcag
    54301 cttttaacgg catcgtaggg atgaaaccta cttatggccg tgtctcacgt tttggtttga
    54361 ttgcctttgc ttcatctctt gaccaaatcg gaccaatgac ccgtacggtg aaagataatg
    54421 cattagcatt gacagctatc agcggttatg acgaaaaaga cggtacttct tcaggcgttt
    54481 cagttcctga ttttgcagag ggactgacag gcgacatcaa aggaatgaag attgctttac
    54541 ctaaagaata cttaggcgaa ggtgtggccc ctggcgtaaa agaagctgta ttgaaagcag
    54601 cagaaacatt ccgttcatta ggtgctgttg tggaagaagt cagcttgcct cattcaaaat
    54661 acggtgtggc tgtttattat atcattgcct catcagaagc aagttccaat ttgcaacgat
    54721 tcgatggtat ccgctacggt tatcgctctg aaaatgtgaa atcattagat gatgtatatg
    54781 tcaactcacg ttcagaagga ttcggtgaag aagtaaaacg ccgtatcatg ttaggtacat
    54841 tctccttaag cgcgggttat tatgacgctt acttcaaaaa agcaggacaa gtccgtacat
    54901 tgatcaaacg agattttgaa aaagtctttg cagattatga cttgatcatc gggccatctt
    54961 caccaacagt tgcattcgga atcggtgaga acattaacga tccaatcaca atgtacatga
    55021 gtgacatctt aacgatccct gtcaacttgg ctggacttcc aggtatgtct gtaccggcag
    55081 gtttatcaga aggattacca gttggattgc agatcatcgg taattatttt gatgaaaaaa
    55141 cgatgtatca agcggcatat gcgtttgaac aagcaacaga attccataaa caacaaccag
    55201 ctattttagg agggaaagac gaatgaattt tgaaacagtc attggactag aagtccacgt
    55261 cgagttaaaa accaactcaa aaattttctc tccagcgcca gctcattttg gtgcagaacc
    55321 aaacagcaat acaaacgtga tcgactgggg ttatccaggc gtgctgccag taatgaataa
    55381 agctgcgctg gaattcggga tgaaagcagc tttggcatta aactgtgaaa tctctaaaga
    55441 tactcatttt gaccggaaaa attactttta cccagataat ccaaaagcat atcaaatttc
    55501 ccaatttgat caaccaatcg gtcatgacgg atggatcgaa atcgaagtag aaggtaaaaa
    55561 gaaaaaaatc cggatcgaac gtgtccattt agaagaagat gctggaaaaa acatgcatgg
    55621 tattggcggt tattcttacg ttgatttgaa ccgacaaggc acgcctttga tcgaaatcgt
    55681 ttctgaagct gatatgcgtt caccagaaga agcatacgct tatctagaag cattgcgatc
    55741 aattatccaa tttacagaag tcagcgatgt gaaaatggaa gaaggctcta tgcgctgtga
    55801 tgccaatatt tctcttcgcc cttatggaca agaagagttc ggtacgaaag cagaattgaa
    55861 aaacttgaac tctttatctt tcgtgaaaaa aggtttagct tttgaagaaa aacgtcaagc
    55921 aaaagttctt ctttcaggag gagaaatcca acaagaaaca cgccgttttg acgaaacaac
    55981 gaataaaaca gttttgatgc gtgtcaaaga agggtctagt gattatcgtt acttcccaga
    56041 gcctgatatc ccgaaattcg tgatcgatga cgaatggatt caaaaagtaa aagctagttt
    56101 gcctgagatg ccagcttctc gtcgcgaacg ctatatccgt gagtttggac ttccagagta
    56161 tgacgcaatg gtattgacac ttacaaaaga aatgtctgat ttttttgaag ctgttttgga
    56221 aaatggtgca gatgccaagc aagcatctaa ctggttgatg ggtgaagttt ctgcttactt
    56281 gaacagtgag aaactggaat tagctgagac aaaattgaca cctgagaact tagcaggaat
    56341 gattcgtttg atcgccgatg gaacgatcag ctcaaaaatc gccaaaaaag tcttccgtga
    56401 attgatcaca aatggcggag aagcccaaga agtagttgaa aaaaatggtt gggtgcaatt
    56461 atctgatccg aacaaactac tgccaatcat taatgaaatt ttggataaca accaacaatc
    56521 tgtcgatgat ttcaaaaatg gaaaagatcg tgcggttggc ttcctagtcg gtcaaatcat
    56581 gaaagcaact aaaggacaag caaatcctgg tgtcgtgaat aagctactta aagatgaatt
    56641 agcgaaacga tagaggctga aaatgaaaaa agcacgcgta atttataacc ccacctcggg
    56701 aaaagaatta ttgaaaaaaa atttggcgga tatcctacag gcattagaag aagcaggatt
    56761 tgaagccagc gcctatgcaa ctacaccaga accagattca gccaagaatg aagcatgtag
    56821 agtcgcagaa ctaggttttg atctagtagt ggcagctggt ggcgatggca caatcaacga
    56881 agtggttaat ggaatcgctc cgcttgaaca ccgtcctaaa atggccatca tcccagctgg
    56941 tacaacgaac gactatgctc gagcattaaa aattcctaga gataatatta aagcagcagc
    57001 agaagtgata aaaaaacacc aaaccgtaaa aatggacatc ggcctttctg ataacagtta
    57061 cttcatcaat atcgctgcag gtggctattt gacagaattg acttatgaag ttccatcaga
    57121 actaaagagt atttttggtt atcttgctta tcttgcgaaa ggagcagaga tgctccctcg
    57181 agtaaagcca atcaaaatgc gcttgaagta tgatgaaggg gaatatatcg gaaatgcttc
    57241 gatgtttttc ctagggctga cgaactctgt tggaggattc gaacagatcg cgccagatgc
    57301 caaattggat gatgggaagt tttctttaat cattgtgaag acagcaaata tctttgagat
    57361 cttgcatatt gcagcactca tgctgaatgg cggtcgacat gtcgatgatc caagaataat
    57421 ctataccaaa acaagcagtt tgttcgttga aacagaagat gatatcaatc gaccagtaat
    57481 gatcaactta gatggtgaat acggcgggga agcaccgatg catttccaaa atcttcatca
    57541 acacattgag ttctttgcca ataccgatca gattcctgat gaagcaatca caggtacaga
    57601 tgaagaagaa ttagaggttg tcagcaaaga attcgtaaaa gaagtcgaac gtttaacgga
    57661 tgaagatatc gacggtgacg gaaaaatagc agacgaaaca cctaatcaaa agaattaatc
    57721 aaaagccggt gctgaacgta gtaaggtcag tgccggtttt agttgtgtaa ttagctgatt
    57781 tatagtagag ttcagtagga ttgaaaaata ggcttaaagg agcaaaatat gacagaacag
    57841 caaatagtaa agaaaaacga acgatataca gtcgaaatcg tcgacttgag ttatgaagga
    57901 atgggggtag caaaaatcga tggttatcca ttatttatcg aaaatgcact ccctggagaa
    57961 gaagtagaaa tactagtagt aaaagcaggc aataagtttg gatatggaaa agtcgaaaca
    58021 tttattacac aatcacctga tcgtcagcct gtagaaaaca atgcattact tcgcataggt
    58081 atcgcacctt tagcccattt gaagtatgaa caacagcttg tcttcaaaca aaaacaagta
    58141 gagcatgtaa tggcgaagat tgctaagatg ccagaagttc ctgtacttcc aacggtcgga
    58201 atggcagaac cattcggcta ccgtaataaa gcacagatcc ctgttcgccg tatcgatgga
    58261 cagttagcca ccggttttta caaaaagaat agccatgatc tagtccctat cgaagatttt
    58321 tatatccaag atcctgtcat cgatcaagca atcaaagtag tcagagatat cttgcaacgg
    58381 ttccaagtac gaggatataa tgaagaaaaa aatgaaggac agcttcgtca catcattatc
    58441 cgtcgtggtc attattccca tgaaatgatg gtcgttttag ttactcgtaa agaaaaattc
    58501 tttaaatcca aggaaattgc tgaagctatt catgaagaat tgccagaagt ggtttcggtc
    58561 atccaaaata tcaatgaaga aaaaacaaat gtgattttag gagacaaaga aaaagtttta
    58621 tatggcagag actatatcga agatcagcta ttaggaaaaa cctatcgaat ctcagcaaag
    58681 tcattttatc aagtaaatac tcaacaagca gagtatctgt accaaacagc catcgagttt
    58741 gccgagttag ataaaaaaga cattgtgatc gacgcttatt caggaatagg tacgattggc
    58801 ttgtctttag cagacagagt ggataaggtg tatgggatgg aagttattcc agaagcaatt
    58861 gaagatgcac agttcaatgc tttaaataat aaaatcgaaa atgttcatta tgaagtcgga
    58921 aaagcagaaa aagttatgaa aaaatggcaa gaaaaaggga tcaaaccatc tgtgatcgta
    58981 gttgaccctc cacgaaaagg attagatcat cattttattg aatcagcaat cgaaatgtca
    59041 ccagaacgaa tcgtctatat ctcatgtaat ccggcaactt ttgcaagaga tgctaagtta
    59101 tttgcagaag caggttatgc cgtgaagaaa gttcagcctg tagatctttt cccgcaaacc
    59161 catcatatcg aattagtcgc ttcattcgtt aaaggagaat aaaaatggac caaaaagaaa
    59221 aaatgaaaga attgatcaaa aagaaacaag gtggaggccg atccaaacaa atggaaacac
    59281 ctaagaatga tcgtaaaaac atgagaaaag gccctaaaat ctttaataaa tagacgtgct
    59341 aaagaaagac taagaagtgt taacttttgt tattgtttct tagtcttttt agttagtcta
    59401 cattattttg gcgatttgcc aataaggact gttgagttgt gcaaaatcaa atctgggtca
    59461 gaatgaacgt cattctgtct cagatttttc gttatttcaa tttgattaac tgattgatca
    59521 aaaagatttt tattttcata ttctcaaatg attcaacact aaatatacat gtttgacgcg
    59581 gaaattatta ttcgattttg gcggcatcag gggggatgac gacgtagcag acccattatt
    59641 ttactcaaga aaatgtccgt gcctattatt tagaatacga tagtgaacgt gctgggagtt
    59701 ttgttctttt agagaaagtc tctgaagata aattagttgt cttagatttt gactgtaacg
    59761 aaaaatggcg aaatggaaga gcaccagcaa gtgattgacc ggatctacga agcgacacga
    59821 tatctgcctt tggatcgttt gtgcttaagt cctcaatgtg gatttgcttc aacagaagaa
    59881 gaaaatgttt tgtttgaaga acaatggaaa aaatttcttt tgtcaaaagc attgcagaag
    59941 aagtttgggg aaaatcatta aatcatctag gagagcttga taggaaataa cactatggta
    60001 taatcagaat gcttaatact aactactgag ggagccttat gaaaaagaaa attcttaaat
    60061 ttatcggatg gttcttcgga atcattcttg ggatcgcgtt tgcaatcttt cttgccttcc
    60121 agctttcccc cagacctgga gcattcttga tcaaccatat gttttcaaat actgttcaaa
    60181 tcactgataa aaaaacgttc gatcaagcag aaaaaaacgt ggcgaaaaaa acagatttga
    60241 cgtatgagtc caagtataaa gagaatactt atgacatcta ctatccaaaa aacagcaagg
    60301 ggcctgttcc tgttctattt tgggtacatg gtggaggatt cgtcggcgga gataaatcgg
    60361 gtgtcaaaga atttgcgaca aaattagttt cagacgccaa catcgcagtg gttgcgatga
    60421 attatgaatt agcgcctgat tcagaatacc ctaatcaagt tcttcaagtg aatgaactga
    60481 tcaaagattt attagctgaa gaaaaagacg acaaattgct agacatggaa caattatttt
    60541 ttggtggaga tagtgcaggt tcacaaatcg cattgcaata tgcagctata caaacgaatg
    60601 atgcgtatgc taaacaaatg aaaatcaaac atctacttcc aaaagatagt ctgaaaggaa
    60661 ccatttctta ttgcggacct gttgatttga agctaacagc acagcagcat tcggatgatc
    60721 gatttatgaa gtttttcgtc aaaaccgttg cgtggtcgtt gattggaaca aaagattgga
    60781 aaacgagtga gcaattgaaa gaagctagtg tggctgacca tgtttctaag gaatttccgc
    60841 caacgtatat aactgacgga aatgcatact cattccagga gcaaggtcaa gcattagaaa
    60901 atcggttgac agaattaaag gtacccgtgc aatccttgtt ttatacagat acaaaaaaag
    60961 agattaccca tgaataccaa tttgattaca cgctaaaaga atcgcaaaat tgttaccagc
    61021 agacattgga ttttgtgaat aagtataagt aagaggttgt gaaaaagccg tttcgttttg
    61081 agtatgaaga aacgaagcgg cttttcacaa cctctctttc aatacctttt tgttcttggg
    61141 ataaaacttt ccctaacaat caatcgtgca ggataagtca aaatgattgg ttcgtcccgt
    61201 ataccgtcaa ttcgctccaa tgccattcga acagctgctt gcccaattga tttcgcattg
    61261 atttgaacac tggtcaagcc aggggagagg tttgcgattg tctcgatatt atcaaagctt
    61321 gctacaacaa tctcttctcc aatttggata tttttttgtt tcaatgcccg atagacacca
    61381 attgccattg gatcgctccc gacaagaatg gcagtaggaa gaggggattc attttctaat
    61441 agattagtcg caagtacctc accttgtttt tctgtccagc cacctagatg ataagacata
    61501 tatttttcta agtgatgatc gatcataaaa tgctgatagg cacgcatcct cttttcattt
    61561 atagaaatct gcttcccacc gaattcatcc atatcgactt tatatccccc gatataggct
    61621 attcttttat gaccattttc taagaacaat tccagtacgg ttttagtcat ccgttcaaga
    61681 tctgcataca ccatatcgac ctcttgctgg atgtcagggt tatccacgac gactatttgc
    61741 atgttttgtt ttaacaaggc ggtaatcgat tctttcgtaa cagtcccaac aacgatcacc
    61801 gcacctaact gatctagatc ttcccattgt ttaggattat cagatagatt atagattcga
    61861 ttcatcccta aatgcagacg tcgagcttct tcttccatcc ctactcgcaa atgtttgtaa
    61921 tagatatcct gttcttcgct cgctttgccg atcgtagaga tcacaccaat cgaaggcatt
    61981 ctctttttga tatatttacg agtactgatg tcataatcca acgcagcagc tgcctctact
    62041 atccgttgcc gagtagtctg agccacatgg atcgtctgat cgttattcaa cacacgggaa
    62101 actgtagcag gagagacatt tgcctttcgt gcgacatctc taattccaac catttttaca
    62161 cactcctcaa aaaaataaaa aatttttatt gacaaaattg tttagtaaac agtatacagg
    62221 aattataaaa aaaagtaaac ggtttccgtt ttgttgggag tgaaatgatg gatattacgc
    62281 atagaaaaac agataaaaat ttcatcttat ttgatgaaaa gagccgagtc ttccatttac
    62341 gaaatacatt tctttcttat ctgataaaga tagaagaaag caatgtattg gcgcatattt
    62401 attttggcaa accagtcaag cagtacaaag acaataaaaa ttatccccga agagatcgtg
    62461 gattttcagg aaatgttccc ttaaatcctg accggtctct gtcgaaagat acactaccgc
    62521 aagaatactc ttctcatggc agcatggact ttcgaacgcc cgcatcgatc attcagcgca
    62581 agaatggatc agacttactg gatctgagat atgacagcca ttatattaca gatgggaagc
    62641 cagagatcga aggcttgcct caaacttatg tgatggacaa gtcagaggca caaacgttgg
    62701 tgatttcact taaggataga gaaacagcga tttatttcga tttgttctat accatattta
    62761 ctgatagagc agtgatcaca cgatctgtaa agataagaaa tgaaaccgga gaaacaatca
    62821 aactagaaaa agcggctagt ttccagctag actttgccca tactaggcga tttgatgaag
    62881 tcattgcttt gccaggagca catgtcaatg aaagacagat ttcacggcaa tctgttttat
    62941 ctggaacaaa agtatttgaa agccgtcgag gaacgagcag tcaccatatg aataacttta
    63001 ttgctttagt tcatcatcat acgacagaaa atactggaga agcgatcgga cttcaatttg
    63061 tttattccgg caaccattct ttcgaacttg aaaaagatca gatcaatcaa ttgagagtcg
    63121 tcggtgggat caacagccat cgtttttcgt gggaattgaa tgcaggccag tcttttcaaa
    63181 cacctgaaat gatcctttct tactcaagtc agggattgaa caaaatgagt cagatccacc
    63241 atgagttgct acgtgaaaga atcgcaagag gtcgtcatca atttgctgaa cggccgattc
    63301 tggtcaataa ctgggaagcc acttactttg acttcaatag tgaaaaaatc aaggcgatca
    63361 ttgatgaagc aaaggagcta ggcatcgaaa tgttcgtatt agatgatggc tggtttggaa
    63421 aaagagatgc ggacaattct tcattaggag actggttcga gtatgaaggc aagttgacaa
    63481 atggattacg tgaaatagca gattatgctc atagcaaagg attgaaattt ggtttatggt
    63541 ttgaacctga aatgatctcg gttgattctg aactgtatcg aacacatcct gattttctta
    63601 tgcaggagcc aggtagaatg ccttctgctt ccagatcaca acatgtactg gattttacaa
    63661 ggttagatgt tcggcagacg attgagaaac aaatgagaaa gattttagat acgattcctc
    63721 ttgactacat caaatgggat atgaaccgtt cgctttcaga tgtctattcg attacccttg
    63781 acccacagag acaaggagaa gtagcgcatc gttatatgct cgggctttat gaactacttg
    63841 aacatttgac aactgattat ccggaaatct tatgggaagg ctgttctggt ggaggtggac
    63901 ggtttgatgc cgggtttatt tattatatgc cgcagtcatg gacaagtgat aacacggatg
    63961 cagtggagcg tatgaaaatc caatatggaa caagccttgc ttatccgatt tcgtccatca
    64021 cagctcatgt atcagctgta ccaaatcatc agaccgacag atctacttcg ttgaaaacaa
    64081 gaggagaaac agctatgagc gctgtttttg gttatgaact ggatttgaca aagctgtctc
    64141 ctgaagaaga aaagcaagta aaagaccaaa ttatatccta tcaaacgatt cgtccggtga
    64201 tccagtatgg ccattattat cgtttggcga gtccttttga agaaaatatt gccgcatgga
    64261 tgtttgtgtc tcctaaacaa gatgaagcta ttgtgttttt agggaggata ctagcttcag
    64321 ctcaaccagc gttccatgaa gtatatctga tggggttaga tgatgaggca ctttatcagg
    64381 aacagacctc gaagcggata ttttcggggg ccgaattgat gacagttgga ctttacttcc
    64441 ccgattttca aggtgatttc caaacagaac tgcttcattt caaaaagtta tgagagagaa
    64501 ggaaaaaagt atgaaagcaa atataaaatg gttagatgat ccacaagttt ttagagtgaa
    64561 ccaatcaaat gcacatagcg atcatgtttg ttatgcaaca agggaagaag tagaaaccac
    64621 atcctccctt aaacagtctt taaatggatc atggcgtttt tgttattcgc caaatccttc
    64681 ggtacgacca aaaacatttt ataaaaaaga ttttgatgcc tgttcttttg atgaaatcac
    64741 tgttcctagt catattgaat tagcaaatta tgacaagatc cattacatca atacgatgta
    64801 tccgtgggag ggacatgtat ttcgccgtcc ccctttcacg acaagccctg agaaagaggc
    64861 ggaagggatc ttttcagctg ctaaagacaa tccggttggt tcgtatttga cgacttttcg
    64921 attagaagaa ggtctaaagg gaaaacaaat ctcgattcat tttgctggtg tagaacaagc
    64981 aatgtttatt tggttgaatg gtcattttat cgggtatgcc gaagattcgt tcactccgtc
    65041 tgaatttgat ttaacacctt atttggaaga agaaaatatt ctagctgtgg aggtttacaa
    65101 aagaagtaca gctgcattct tggaagatca ggactttttc cgattttttg ggatcttcag
    65161 agatgtcttc ttagttgctc gaggacaaaa ccatttagaa gatctttgga tcaaacctcg
    65221 cttagatgac aaatgtcaaa atggcaaact tgaagtagat ttgtcttttg agcaagtagc
    65281 caaaaacagt cagctgaatg tagcgatact agatcctgat gggaaggaaa tctatcaaca
    65341 gcaatatccg ttaaaagagc aaatgacgat tcgcatcgat ggatttgaaa atgtggcact
    65401 gtgggggcat tttcagccca atttatataa gcttgaattg gagacgatag acgaagaaaa
    65461 aagagtaacg gagtatattg actattcctt cggttttcgt aaaatcgaaa tcaaagataa
    65521 aatcatctac ttcaatggaa aacggatgat tatcaacggg gtaaatcgac atgagtggca
    65581 tccgaaaaaa ggacgcgcca tcactataga agatatggaa aaagatttgg ctatcataaa
    65641 aaatgcaaag atcaacggcg tacggacatc tcattacccc aaccagatca cttggtatta
    65701 tttatgcgac caagcaggaa tccatgtgat ggcggaaacg aacctcgaat cccatggctc
    65761 atggcaaaaa atgggcgcat ttgagccttc ttacaacgta ccaggtgcag tacctcagtg
    65821 gcaggcggct gttcttgatc gagcaaaaac gaatttcgaa acattaaaaa accatccaag
    65881 tattcttttt tggtctcttg gaaatgaatc ttatgcggaa gaaaacattc gacaaatgaa
    65941 cgattacttc aaaaaagtag accccgatcg acttgtccat tatgaaggga attttccaaa
    66001 tccagcattc gaagattcga tttcagatgt aaaaagctgc atgtatgcgc caccagaaga
    66061 gatcattgac taccttgaaa ataaagcgga aaaaccgttc atcttatgtg aatatatgca
    66121 tgacatgggg aattcgttag gtggaatgga ttcttacatg acgttgcttg atcgttatga
    66181 aatgtaccaa ggaggctata tatgggattt taaagatcag gcgatccaag tgactgatcc
    66241 aatcacaggc agagaagtga tgcgctatgg cggtgacttt gacgataaac caagtgacta
    66301 tgaattctca gggaatggga tcgtttttgc agatggacaa gaaaaacccg ccatgcagga
    66361 ggtaagatat tattatgaaa aatacagtaa ataaaagtca tatggatacg gaaaaagttg
    66421 caatcgtctt cggcgactgt acattaggtg tcaaatcggg gaatacgcat tatatttttt
    66481 cttatacaag aggcggactg gaatcgctca ataaaaacgg aaaagaatgg ctataccggg
    66541 aaacacttcc agttttttgg cgagcaacga ccgataatga cagaggtagc ggatttggat
    66601 ttaaatccgg tatgtggtta ggagccggat tgtatccaaa agtcaaagaa atagaagtac
    66661 tggtggacaa taaaaagatc gatttgccga ttgctccagt aaataatcaa tattcaagta
    66721 atgaatacgc tgaaacggtt gaaatcattt ttacactgga aatcaacaca gtaccaaaaa
    66781 cccaagtgac aatttcctac caaatcgctg gagagggtaa aatgcttgtc aatatgcatt
    66841 attatggaca aaaaggatta ccggaattac ccgcattggg tttaaggttc attatgccaa
    66901 cgaaagcaaa attttttgag tatgaaggcc tatcagggga aacatatccg gatcggatgg
    66961 ctggtggtgt cgaaggtatt tataaagtgg caggactccc cgtttcccca taccttgttc
    67021 cccaagaatg cggcatgcat atggaaacga aatgggtaga actaacaaga aatcaaacgt
    67081 tggataatac ggataaagat caagaagatt tttcgttgcg atttgaacag caggatacgc
    67141 catttgcttt ttccgcttta ccattcaaag cagaagaact ggaacaggca acacatatag
    67201 aagaactacc gccagaaaga cgaacggttc ttacgatttt tgcaaaagta agaggagtcg
    67261 ggggtatcaa tagctgggga gcggatgttg agtcacctta ccgaatcagc agcgaagagg
    67321 accatgaaat cggctttatt attcagtagc aatctttaga ttattatttg aaaaacggag
    67381 tttacaagac aaagctgctt tattctatat gtcatgaaca agagagtaaa aaagttctca
    67441 gatacttttg ttcatgacta ttttcttcaa taaaaccaat tgttattttt ctgaaaaaca
    67501 aagttaacta tcagaaaatt aaaaaaagtt tcataaaaat gattgacagt cgattttttt
    67561 atgtctatac taggtacaat tcttacatca gtttggagcg attttttatg aaatctagaa
    67621 aacttacaaa actgaatacg tattacttta gctattttag ataggtttgt attcatgact
    67681 tcgtgggtac aaacaaacca ggtttgtatc tatgatggct aaagattttt aaacacgact
    67741 tacagccacg tagagaaatt catctatagt ggctatttta tatttttgca gcgcttttta
    67801 aggctcgttg aagatgttca ctgtagaggc aatttcctac agggacatct ttttttgttg
    67861 attatctggg tcataagtag gaaagaagga gcgaaaatga gaacaacagg acacggtcat
    67921 ttagtaggaa ttaatgtaaa accaccttga aaaagtagag aaaatcagcc aagatagata
    67981 agtaaacaaa agaaagcggg aaaaacaatg atagtaatta tgaaaaaaga agcaacggaa
    68041 gaacaaataa aaaaagtcgt cgatcatatc gaaaaagaag gattcaaagt ctcaatcgat
    68101 caaggaacag aacgagtagt gattggacta aaaggtgata cgcgaagttt acaagaagga
    68161 gcatttacac gctatgaagg agtagaaaat gctgtgcgta ttttaaacac atacaaattg
    68221 acttctcgag aattccatcc tggcaataca gtcgtggatg tagacggagt aaaaattggt
    68281 gacggcagtt tcgtgacgat ggctggtcct tgttctatcg aaggattaga ccaaatcaga
    68341 gaatgtgcgc gaatggccaa agccgggggc gcaaaaattc tgcgaggtgg tgccttcaaa
    68401 ccacgtactt ctccatatgc tttccaaggt ttagaagaag aaggattgaa atacatccgt
    68461 caagcggctg atgaatttgg aatgaaagtg attactgaag tgatggatga agggcatatc
    68521 gatatggtgg ctgagtacag cgatatccta cagattggcg cacgtaatat gcaaaatttt
    68581 aaactgctat ctgctgtcgg taaaacaggt aaacctgttg gattgaaacg tggaatttcc
    68641 ggtacgatca atgaatggct taatgctgca gaatacattg cagtcgaaga caaatctcct
    68701 gtcattttta tcgaacgtgg gattcggact tacgaaacag cgacacgtaa tacgtttgat
    68761 ttaagtgctg tcccattgat gaaaaaatta actcatttcc ctgtgattgt tgatccaagt
    68821 catggtacag gaatttggga acttgtacca ccaatggctc gtgcgggtgt ggcaagcgga
    68881 gcagatggca tgatcgtaga gatccatcct gatcctgcaa atgcttggtc agacggtccc
    68941 caatcgttga atgaaaagac ttactcacgc atgatgaaag aagttgcgat catgaaagaa
    69001 gcaatggaaa agattcagac actcgattaa ttccagcttt ttaaaagaaa gaagtgaaac
    69061 gaatgcttac tgtacatgtt gataaccaaa aatacgatat tcatattgaa aaaggcgcac
    69121 tagaacaggc tggatcatgg cttcaaacga tttggagacc tcaaaaagta gctgtgatca
    69181 ctgatacaaa cgttgctcca ctatatgcag aaaaaatcat gttccaattg agacaagcta
    69241 attttgaacc agtcctttca atcgtaccgg cgggtgagac cagtaaatct ttagaacaag
    69301 cagctgagtt atacgatttt ttagcagata atgattttac ccgcagcgat gggatacttg
    69361 cacttggtgg aggaatggtg ggtgatctag cagcattcgt ggcctcgacg ttcatgcgtg
    69421 ggatccattt cgttcaaatt ccgactacgc tacttgcaca agtagattct agtattggcg
    69481 ggaagacagc agtcaataca aacaaagcga aaaaccttgt cggaacattt agccagccag
    69541 ctggtgtatt gattgatcct gaggttctaa aaactttgcc ggtacgtcgt ttacgagaag
    69601 gaatcgctga aatcatcaaa tcagcagcta tagcagatgc aacgctttgg aaacagctga
    69661 acgagctgaa agacgaaaaa gatcttctgg aacatgctgt tccaatcatt actgctacgt
    69721 tggaaatcaa acgaaaagta gtggaagaag atgtctttga ccaaggaaat cgacttattt
    69781 taaattttgg tcatacaatt ggccatgcaa ttgaaaatac agcaggatat ggggtagtca
    69841 gtcatggaga aggagtagct attggaatgg tagccatcac ttctcatgca gaaaaaatcg
    69901 gcttgtctcc aactggatcg acagctgctt tagaaaggat acttcaaaaa ttccatctgc
    69961 cgattacgta cgatctaagt catccagatg aattattcca cgcgataacc catgataaga
    70021 aagcaagagg aagccaattg aatatcatct tgctagaaaa gattgggcaa gcaaaaatcg
    70081 ttcctgttcc cacagagaca ttcaaagatt acttaggagg actgtaagat gcgttatatg
    70141 actgcgggag aatcccatgg accagaatta acagccatta ttgaaggact accagcagga
    70201 ttacctctgt cagaagaaga aatcaatcac gaactcgctc gcagacaagc tggttacggc
    70261 agaggaggaa ggatgaagat cgaaaaagat cgtgttcgga tcacgtctgg catccgtcac
    70321 ggcaaaacaa ttggttcacc tgtcacttta gtggtagaaa ataaagattg gaaaaattgg
    70381 acaactgtca tgtctgtagc agaagtgcct gaaaaagacc aatcactgcg gcgagttgca
    70441 cgtccgcgtc caggacacgc ggatttggta ggcggtatga aataccatca tcgagatctt
    70501 cggaatgtgc ttgaacggtc ctctgcaaga gagacgacca tgcgtgtagc aatcggcgca
    70561 attgccaaga aaattctgga agaacttgac atcatcgtag caggacacgt gatttgtctt
    70621 ggtggaatcc aagcagaaat acctggaaat atctcgattc aagaaatcaa agaaatttct
    70681 gaagcgtcag aagtacgagt tgttgatcca gcagttgagc aaagaataaa agatttgatc
    70741 gatcagacaa agaaaaatgg agatacgatc ggaggaattg tagaagtgct cgttggaggc
    70801 gttccagctg gattaggaag ctacgtacaa tgggacacga agctagatgc caaaatcgca
    70861 caagctgtgg ttagtatcaa tgcctttaaa ggcgtagaat ttggggtcgg attcacttct
    70921 ggttcattac caggctcaga agtgatggat gagatcctat gggaagaaga aagaggctat
    70981 ttccgacgta ccaataatct aggcggcttc gaaggcggaa tgaccaatgg cgaaccactt
    71041 gtcattcgcg gggtcatgaa acccattcct acattgtaca agccacttca aagtgttgat
    71101 atcgatacaa aagaacctta caaagcaagt gtagaacgtt cggatagtac agcagttcct
    71161 gcagctagcg tcgttgcgga aaatgttgta gccactgtgg tagcgacaga gctattgtca
    71221 aaattcccaa gtgattcgct tgttgagttg aaagctgcag tgaaggagta ccgtgactac
    71281 ttgaaacatt actaaaaagg gggcaaataa gatggcacaa catgttttta tcgtggggct
    71341 tggactgatt ggtgcttcta tggcactttg tattcgtcag accaatccag aactgatagt
    71401 agttggttgg gatagtcagt caaaaacccg tgagcaagca gtagaacagc agatcgttga
    71461 ttacgttgca agtgattttg aaacaggtgc agaacaagca gacgttatct tattagccgt
    71521 tcccgtccgt acaaccttag cttatcttga tgtccttgaa agaatcaact tgtctgatca
    71581 cgtcatcatt acggatgtca gcagtacgaa gcagcaagtc gtgacatccg ctgagcagaa
    71641 aaatcttcgg tttgtcggtg gacatccaat ggctggttct cataaatcag gtatacaggc
    71701 agcagatgct aaactatttg aaaatgccta ttacatcttt acacctttgg aaaagacgaa
    71761 aaaagatgtt gaaaaactgc aagaactatt ttgcggaacc aaagcaaaat atgtaactct
    71821 tacagcaaga gaacatgacc gtataacagg aatgctaagt cattttccac atattctggc
    71881 agctggctta gtcaatcagg cagaacaatt caatcaagag tatccgcgag ccaaacaact
    71941 ggcagcagga ggctttcgag atatcactag gatcgcttcc tcagatcctg tcatgtggac
    72001 agatattttg ttgagcaata agcagatatt attggaacga ttggcagatt ggcaacaaga
    72061 aatgacacaa atagcagaat ggatcatgac tgagaaccaa tcggaaatct tttctttttt
    72121 caatcgggca aaagaaagtc gagaccagtt gcctgtccat aagcaaggcg cgattcctgc
    72181 cttttttgat ttatttatcg atgtgccaga tgaacctggc gttattgcag aagtaactgg
    72241 actgatcgga aaagcgggag tttctttgat taatttgaag atccaagaga ccagggaaga
    72301 tattttagga atactgcaaa tcagttttaa aaaccaacag gatttgttac aagcaaaaag
    72361 aatcattatg tctaatacca gttatcattg ctggatcaaa taggaggctg gataaatgca
    72421 attattacaa caaatacatg gattaagagg gactgttagg ataccagcag ataaatcaat
    72481 ttcccatcgc agcatcatgt ttggagcaat tgctgaagga acgacgacta tacaaaattt
    72541 tttgcgcgca gaagattgtc tgagtacttt acatgccttc caacaattag gtgtcgagat
    72601 cgaagaagag gaagaggtga tcaagattca tggtcgcggt agccactcat ttgtccaacc
    72661 aaccgaatca attgacatgg gaaactccgg tacgaccata cgtttattga tgggtatttt
    72721 ggctggacag ccttttacga ctactctgtt cggggatgct tcgttgtcta aacgtccaat
    72781 ggggcgagtg atggaacctt tacgcgagat gggcgctgac ttgcaaggag aagaaaatga
    72841 ccagtatcta ccaatcactg tgacaggaac ccgctcttta tcacctatcc gatacaatat
    72901 gcctgtagcc agtgcgcaag tcaaatctgc tttgctgttt gcggcattac aagcagaagg
    72961 cacatccgta atcgttgaaa aagaacgttc ccgtaaccat acggaagaaa tgattcgtca
    73021 atttggcgga aggatcacag tggaagataa aacgatcata gtgaccggac cgcaaaaatt
    73081 aactggtcaa cagataactg ttccaggtga tatttcatca gctgctttct ttctagcagc
    73141 aggacttctt gttccggaaa gccaactgtt gttaaaaaat gtcggggtca atccaacaag
    73201 aactggtatc ttagatgtgt tggaggagat gggcgcagcg attacccaga cgaatcacaa
    73261 tgaacagaac caatcggctg atttaagtgt caagacgagt catttgaaga aagcaacgat
    73321 tggtggagaa atcattccgc gtctgattga tgaattgccc attctagctt tagtggctac
    73381 tcaagcagaa ggaatcacga tcatcaaaga cgcagaagaa ttaaaagtaa aagaaaccaa
    73441 cagaattgat gcggtggcag aagaattaca gaaaatggga gcaaagataa aagcgacagc
    73501 agacggtctg atcatccatg ggccgacacc tctgcatggc gcaaaagtgt ccagcagagg
    73561 cgatcatcga atcggtatga tgctgcaagt agctgctttg atagctgatg gaccttgcga
    73621 actagaagga gctgaagctg tttcaatctc ttatccggca ttttttgacg atttagcaga
    73681 acttgtgtca ggaggagaag ctcatggcta gcattttgct gattggtttc atgggcgctg
    73741 gaaaaacaac aatcggtaaa gggttagctc aatgtttaca aaaagagtat gtagatttag
    73801 atacaaagat tgaggaacat atccaattgt cgattgccga atattttcac tattacggtg
    73861 aaaaaagctt tcgaaaagtg gagtcagata ttttaagaaa gctgtcaaat gaagacaaaa
    73921 ttatcgcgac tggtggaggg attgtccaaa gtgcagaaaa tcggcgtttt ttaaaaacac
    73981 agccgattgt tctttactta gaggcagaag cggactgtct agttgatcgt atccaacaag
    74041 atgaaacgtc tattcgacct ttagctcttg gaaaaacaag agaagaaata aaagcattat
    74101 tggcacaacg actttcttgg tatgaagaaa gtgcgacaca tcgaatcaac acgaccaatc
    74161 aaacaccaga aaagatcatc gataccatta tagaaaggat caaaagcata tgaacgtcag
    74221 ctatctaggg ccagaaagtt cattcactta tcaggcggct tgtcagctat ttcctacaga
    74281 acaattgact gcgtatgctt ccatccctgt ttgtttgcgc gctttgtttc gcaaacaagt
    74341 cgatctggct gtcgttcctg tagagaattc attggaagga gctgttcatc acacaatcga
    74401 tcttctttcc aaacatccag aagtcgaagt taaaagtgaa atcgttcttc ctatcaagca
    74461 gcagctttta ggcaatccag caacaaagat tacaaaaatc ctttcacatc cacaagcact
    74521 agcacaatca caacagtttt tagaaacgca ttatcctaat gttcctttag tagcaacaga
    74581 atcaacaact gctgcagcaa tgtatgtagc agaacatccg aaagaagacg cagcagcgat
    74641 tgcttcatta gaaacagcac agcatgttgg attggagatc ttagctgaga atatccaaga
    74701 taatgagttg aaccagacac gtttttggat cgtcggtgac cgaaagatga ccagccagca
    74761 gtcagctcca gtaaaaatga gtgtcatctt gacactacct gctaatcggc cagggatgct
    74821 tcataaaatg ctggcagctt ttggctggcg ggaaatcaac ttaagtaaga ttgaatcccg
    74881 accattgaaa actagcttgg gagaatattt tttcgtgatc gatttgttgt tggaccgtcc
    74941 aatgacatta gtagagaatg cgctccaaga aatcaagatg ctcggaggag aaagtcaaat
    75001 attgggctgt tatccagtgc tgacagtaga agagacgcgt agatgaaagt caagaatact
    75061 gattgattta tttaacttaa aataaaaaaa tcagcgcttg gctattcttt atttttgata
    75121 ttctgattga tttttaggaa gaagcggtag acaaatcgtt acagtcgttc ctgtcccaat
    75181 cactgattgg atcgttaagc catatgcatc gccatacatt aggcgtatcc gctcatctac
    75241 atggctgatg ccgattcctg aaaaatgatc atttttttgt ttggtatttt cagtatcgaa
    75301 gccatcgccg ttatcaatca cttcgaataa aagaagctga ttttttgttt tgccgtacac
    75361 cgtgatgaat ccttgcgttt tattgtgaaa agcatgaaga taggcgtttt cgataattgg
    75421 ttgaaggatc atattcggca tagataatga ctgcagttca tcaggaatca ttattttagc
    75481 cgagattctt tcgccatatc gcatactcaa aagatcgatg taactttgta cgcttttgat
    75541 ttcttccttg actggaatca cttcttcttt tttcccaatc gtacttctta gtaaagtaat
    75601 gaaactgtcg attccttgaa ctgcttcatc tgtttttttc tgccaaatca aaaatttcaa
    75661 gctagtcagc gtattataaa taaaatgggg ctgtatctgt gcttgcatag tttggatttc
    75721 aagcgaacgt ttcttctttt cgtcttggat cgtctgttca taatgatgct gagcattact
    75781 tagcaattga ttgatcgtat tccctagcaa ttggacttca tggcttcctt gtacaggaac
    75841 agtagctttt aagccatctt cctctagccg ttttagcgaa tcaatcaagc ggtagatcga
    75901 ttttgtgtgt cgatccaaat acataaatac caatgagcca gcaaaagcaa gtgtcacgat
    75961 attatacaaa ataatcggaa aaatcacgcc catttggtta gataaagaat gaagatcgac
    76021 ttgactgagt aatgtacaat cctggcggta cagaggaaga cgggtcaact gctgtccatt
    76081 tgaaatcgtt gtttcaccta ctgttagttt tttgaaaatc attgatcggc tcccaattgt
    76141 attttcttca tttgatgcga tgatcgtctg ttcttcgttc atgaaggaga tcttcgatat
    76201 ctcaggattt actacagatt gaaaaagatt cgctagttgc ttggatgaga tcgataaaac
    76261 agcatatccg taaacgtgat tcaatgtatt tttcagtttt ttgacaaaga agacgctggg
    76321 tgctttctta tcgcgaagag tcaatccgtg gtgtgcaaaa agcatttgtg tatcagcagc
    76381 acgttcattc actctctgag caatcgcaga ttgaaaaaaa gaatcggcag atacggcagt
    76441 gagcatgtcg tttccgacaa aagttcttcc attttctcca aagatcaata catcgtaatc
    76501 gatcattgga tggatttttg aaagagagtc attcattttt tggattgttc ttagttgctg
    76561 aagctgttcc atatgttggg ctggatcatt ggaaaaatag cttttgacag ctggattaga
    76621 atcaaaagcg gctaatgtat tctcgattgt atcattcaaa ctttcaaatt cagattgaat
    76681 cttttttagt accacatcat tcgattgatt gaacagctgg atataggcat cccttgatcg
    76741 atgataaata aacaaagaaa tgacgatggc ttgcagaaaa agaccgatga acaaaatgaa
    76801 cagaagtttg gcaaagagtt catgctcttt gatttttttt ctcattttac aaaagttcct
    76861 ttacggtatt gagaaggtgt cattcctgtc tcttctttaa aaactctgga gaaataaccc
    76921 agttcggtat agccgacttt ctcacagatt tccgtcaaag taagccgtgt ttccgtcaaa
    76981 aagagcttgg ctttactgat acggactttt tttagatatt ttgaaaagtt gataccaaat
    77041 ttttcagtaa agatggctga gagatagcta tatgaaaaat gatatttttc ggataaaagt
    77101 tgtaaattga tttctttctc ataattttcc tggacgaact cgtagatcaa agtcaagata
    77161 tcgaaattgt ctttttttgc atttgcagaa aagtgggtga gtttctcaag tgtttctttg
    77221 acaagaatag aaaaatcttc gaaatactca gcatcggcta ataaagaagg aagggcgatc
    77281 ttgagcaaag aaagttcttc attcccaaca tgctcgtcag ccaaagcgtt gagtaatgca
    77341 tagaaacgat tgattgcctc atgtttcaaa taagctggat caataggctg ttccaatatc
    77401 gtcgtgaaga aattctcaag ttctttgaca gctaaagcgt agtctttatg aacgattttt
    77461 gttaaataac tacttgcttg ctgctcgtgg ttggttaata acggaagaag ttcatgctga
    77521 taagcaaggt tctttccctt atgatagaaa cgttgtcctt tggtgcaatt tctgatctct
    77581 tggaatattt cattgatatc ttggattgca aaagaatcag aaacaacaaa aaaatcacct
    77641 ggaaaaactt cttgacgaaa cttttgaaca aacttcctaa gtgtatcttt ggaatcagta
    77701 ttgattacat agccggattc ttccgaaaag gtagaaacag gataaatagt aaacgaatcg
    77761 tcgctatcca tcaatgaatg ggtgatcgac tgtttttgtt tagagccttg ttttgaactg
    77821 aatggccgtt tttcggtata taacagaaaa acaaggtgtg ttcaaaaaga agagggtcaa
    77881 gcaatggacg agtttgtcca gcaatctgct gtgaaagatt ctcgcagatc tgctcataac
    77941 gattggtgat tgggcttttg ttgggctttt ttttgatttt ttgtgaaacg gcagttaagg
    78001 aatttagaaa aaaatcagga ctcaatcgag gctttaaaag ataatcagcg acgccttctt
    78061 taaaagaatc tttaaccata tcaaattcat catagctaga gaggataagg atctgtgttt
    78121 caggttgttg ctgttttaat agccgcgata attcgattcc atttaattca ggcatcatta
    78181 catctgtgac cacgatatcg attgcatgta ccaaagaaaa atcaagcgct ttcagcggac
    78241 tggaaaaagt ccctattaat tgaaacccgt gctcagacca attgatcaac tgtttcattc
    78301 catctaaaat cgtctgttca tcatcgataa gaattacgcg tattggcatt tttttacccg
    78361 ccttaagaat agtcttgtaa tttaaaaata cttatttcta ggagaaaatg caaggatatt
    78421 tttattttaa agaaacctca ccatttcgat ctttggtcta gatgctaaga ttattccttt
    78481 tgtcatagtt gatttgtatt tttttattat tttaggagaa atttttgaag tatctgaata
    78541 ataaagaaac cgttttcaaa ttttggtatg atgaatgtag caaacgtaaa caactaatgg
    78601 atggaaaagg agcggataaa aatgaacatc agaaagttaa ttgctggagt cgtttgttta
    78661 gcggcagcat caggtttctt aacaggatgc ggaagtgcta gtagtacagc agacaaagaa
    78721 gaaaaagtca ctgtctgggc gtgggatgag acattcaata tcaaagcagt gaatgaagca
    78781 aaaaaagttt atgagaacga agaaacagag atagaagttg ttacgatgtc tcaggatgat
    78841 atcgttcaga aactgaacac cgctttagcg agcgggaata cagaaggatt gcccaatatc
    78901 gtcttgattg aagattatag gatacaaggc tatttgactt cttatccaga tgcattcgct
    78961 aatttgtctg ctattgtaaa ggaagatcat tttgcaccgt ataaatttgc ggtaaacaaa
    79021 gtaggagata aaatatacgg tgttcctttc gattcgggcg taacagcaaa cttctatcgt
    79081 acggatctga tggcagaagc aggttataca gaagaggata tggagaatct gaagtgggac
    79141 gagtatattc aagtagcgcg agatgtcaaa gaaaagacgg gaaagaaaat cgctgaagtc
    79201 aatccttctg accttggtcg agtaaggatg attatgcaac aggcaggaga atggtataca
    79261 tctgaagatg gggagacagt cacgatccaa gacaatgcat ctttaaagta tggtcttgaa
    79321 ttgtttgcca ctcttttgaa agaagatcta gtcgaacaaa cctctgactg gaatgctgga
    79381 gtaactgcga tacaatcagg agcagtagct tctagcccaa ctggcgcttg gtattcttca
    79441 acgatccaag gagcagaaaa ccaatctgga aaatggaaga ttgcaccaat acctgcactt
    79501 ccagaaaatc tgcaaaaagc gcaagcttct aaccttggcg gcgctggctg gtatgtgatc
    79561 aaaggagtag ctggagaaga ttcagcgaaa gatttcctag aagcaacgtt tgcttcgaat
    79621 gaagaactga tgggcactct tgcgaaagaa atcggactag tatctacgat gcttagcgcg
    79681 aatgaacaag aagcttatca agaagcttct gaattttaca gtggtcaaaa gatctttgac
    79741 gatttttcaa catggacatc tgaaatacca gaagtgaatt acggacatga gacctatgca
    79801 attgaagcag tcgtagctga agctcttcat cgaatcatca atggagaaga gacagacaag
    79861 gtactagcag atacacaaaa acaagtagaa gcacaattgg ctaattgatc ggtggtaaaa
    79921 aagtgaattt cttcgctttt tcatcagaca aacgaaaaga caaaaaagat cacgtctgcc
    79981 ttacagatat tgtctttctt gtcttttcat gtagataatc aatgatagaa ttggagggaa
    80041 aagaatgaag cagaaagatt tattcaaaaa cgggcatgct tttttaatct tgccagtcat
    80101 tttgatatca atcatggttt ttattccgat ggttttggct ttgatcacgt cttttcaaag
    80161 tggtccccct gtcaatatga catttaacgg gttaggtaat tatcagcgga tgttgacaga
    80221 tgcaactttt aaaaaagctt tttttaatac attcctgtat ttacttgtac aagtaccgat
    80281 catgttgttt ctagcacttt ttatttccaa ccttttaaat gataaaaagt tgaaatataa
    80341 agggttgttt cgaaccgcta tctttcttcc atgtatcact tcgatggtca gttattctct
    80401 gatcatgaaa agtctatttt ctcaatctgg ccttgtaaac aacttgttgt tgggcattgg
    80461 tatgattgac acaccgattg gctggttgac tcatccaatt tgggcgagag ttctgatcat
    80521 tctttcgatc acttggcgtt ggactggata caatatgatt tttttccttt caggcatgca
    80581 aaatattgat tcggagattt atgaagcagc atctattgac ggtgcttcta aatggcagca
    80641 gtttatcaaa gtgacgatcc caaatttaaa accaatcatt ttgtttactt ccattacttc
    80701 gacgatcggg acattgcagc tttttgatga agtacaaaat attactggcg ggggaccagc
    80761 aaatgcaacg accaccctat ctcaatacat ctataatttg agttataaat tcacgcctaa
    80821 ttttggatat gcggcagcag tttcctttgt tattgtttcc tttattgtga tattgtcgat
    80881 aatccaaacg aaagtaggag gagaaagtcg atgaaaaata tggtagaaaa agaaacaatc
    80941 gaacgcccca acgtcaaaaa acaagcatta gaacttcgtt cgacaattgg gtcagtatta
    81001 aagtatacag cattaagcat cctatcattt atttccattt tcccttttgt ctggatggtt
    81061 ttgggaatga cgaatacacc cgtggatatc acatcgggaa aaataaagat tggcagtaac
    81121 ttgatcatca attttcaaaa tttgttttcc aatgagctga attttactcg ttccctttgg
    81181 aattcagcga tgatcgcttt ggtgaccact gtgtttgcat tgctgatttc gtcaatggca
    81241 ggatatggat ttgagattta tcaaagtaaa cgaaaagacc taattttcaa tattctactg
    81301 ctatcgatga tggttccttt tgcggcattg atgatccctt tatatcgaat gttttcacaa
    81361 ttgaacggaa cggttttggg aatcaacagt ttcttggttg tcatcctgcc atctgttagc
    81421 actgcttttt tgatcttctt ctttcgacaa aatgttaaat cttttccaaa agatctagta
    81481 gaagccgctc gattagacgg tctaaaagaa ctaagtattt tttttagagt gtatatgccg
    81541 acggctaaaa atacctatgc cgcggctgca atcatcacat tcatgagtag ctggaacaat
    81601 tatttatggc ccttagtagc tttgcaatct cctgataaac ggacggttcc gcttgtttta
    81661 tctgctatgg gtgcttccta tacaccagac tatggaatga tcatggctgg attagtcatc
    81721 gctactttac caactgccat cgtatttttt gttttgcaaa agcaattcgt tcaaggaatg
    81781 atcggctctg taaaataaaa aaacacaata agcgcgttcc ttcaaagtca tttttccaag
    81841 taaaaagata cttttgggac gtgtgttatt ttatgttgag aaaaattaaa tgataacgtt
    81901 ttaagtttgt gctcgaaaac tctttcattt taaggtaaac ttaaagagta aaggaggagg
    81961 atacatgcta aaaaatttta aagaagtgat tgctgctata ttgccgatga ctgtcctgat
    82021 tgtcatttta acgtttgttt ttgcaccgtt agaaggagaa gacctcgctt catttctggt
    82081 tggtgcggca atcatgatga tcggaatgac gctctttttg tttggagcag attattctat
    82141 gatggaagtc ggagatctag tagggaaata tatgatcaaa aagaaaagtt tgaccgtttt
    82201 ggtcagtttg ggatttgcta tcgggatcgt gatcacgatt gctgaacctt ccgtgcaagt
    82261 tttgggacaa caagtatatg atatatctga cggagagatt ggcagagtgt tgctgattgg
    82321 gatcgtcagc gtcggcactg gagtatttct agcctttgcg ctgcttcggg tcgtattcaa
    82381 gctatcctat tatcaattga tggctatagg ctacataggc gttttgatcg cctcattttt
    82441 caccagttcc gaatttatgc cgattgcttt tgattcgggg ggagtgacta ctggtccagt
    82501 aactgttcca tttatcttgg cccttgccgg agggatgact agtatgatca aacaaaagaa
    82561 aaatgaaaat gacagttttg gaatggtcgg tattgcttcg ttagggccga ttcttgccgt
    82621 gatgattttg ggggtgatct tccaatgagt ttctggcagc aagtgataga tggaatcgaa
    82681 atcgttacca tggaagtcat tttggcagtg gcacctatcg taatcctatt tgttttaatg
    82741 aatatcatta gttttcgtat gcgcaaaaag cgtttctttg acattatgaa aggtttcttg
    82801 ataacgacta ttgggttgat ccttttcctg cacggagtca atatggctta tgtaccggta
    82861 ggacagcatt taggcagtgc tattgcggga ttgccttaca attatatact ggttcccctt
    82921 ggctttttaa tggggttctt agtagggttt gctgagccgg cgattcatgt aatgatcaaa
    82981 caggttgaag aattgagcga ggggagaatc cgatcgaaaa tcatgctgat ggtaatttca
    83041 attgggatcg gcgcagcagt aggattgtct atgtggcgtt tgctcgtcgg attctctctt
    83101 tattatttct tgatcccagg atacgggcta gcatttattt taggacgaaa agtggacaag
    83161 atctttttag caatggcttt tgataacggt ggagtagcaa cgggcccgat gtgttcgaca
    83221 tttattttat caatgagcgt ggcaatcgct gcacaaattg aaggaagaga tccgatcatt
    83281 gacgggtttg gtgtggttgc aatgattgca ttgacaccaa tattatccac tttagtacta
    83341 ggatatattt ataaacgaaa agatgaaaaa gacagaatcg aaaaagaaag attgcaaaaa
    83401 ttgggggaaa gcaaatgaaa gatcttgtag cattgaatct tcacatgatt gtgacgatcg
    83461 ttgacggagg aatgggcgaa gatgttatcg acttttcaaa agaagcaggt gcagcaggcg
    83521 gaacgatctt acatggaaga ggttctggtg tacatgattc agggaaattt ttaggtttgg
    83581 acatttcgcc agaaaaagaa tcaaaagcag cgtgcaccta aaggaaaagg acgaatacgt
    83641 gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag aaaagaatga
    83701 aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac tggacaggat
    83761 ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc ggtcaaaaaa
    83821 tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa cgtgcgattc
    83881 ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata ataaaaacat
    83941 ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat aagatgaaag
    84001 aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa gttccgccgt
    84061 taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat ttgatcccat
    84121 acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa gatcatctag
    84181 caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat agcatccgag
    84241 cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc gatatcccaa
    84301 atgcacaacc aatccagtat acattagcag atgatttgac aattctggac aaaaaaatac
    84361 tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag gaatggaaca
    84421 aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat acgtatcccg
    84481 caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg gacaaaaaag
    84541 aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa tcaaacttga
    84601 atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg gaagtttccc
    84661 gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc ggtcgtcatt
    84721 tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa catcgattgg
    84781 cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa cctgatccag
    84841 catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg atgatgatcg
    84901 gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact tgtctttacg
    84961 acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat ctgaatgaaa
    85021 tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag atatgttata
    85081 attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga agttgtagaa
    85141 gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt gaaagtaggc
    85201 ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat cgtaggagca
    85261 cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt gagtcagatc
    85321 aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga agaagtggac
    85381 gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt gacagagatc
    85441 gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga atagacagta
    85501 aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta ctaattgtaa
    85561 gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact gaaaaaaatt
    85621 ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc gatggacttg
    85681 tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta gtggttctag
    85741 tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca aaattttcaa
    85801 cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt caattgatct
    85861 ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt gtaggtcaag
    85921 gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg atcattggtg
    85981 cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact gtattgactt
    86041 ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca atgctgttcg
    86101 gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc tctgaactgg
    86161 caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta tcctttatcc
    86221 ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt ggagtcgtta
    86281 ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg ccaaaagaag
    86341 cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg atcgtgacgc
    86401 ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt ttggcaacaa
    86461 gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac gtagcattgc
    86521 ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat ccaatcaact
    86581 atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca agtgctggag
    86641 ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat gcaacagcta
    86701 atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc gtttatccag
    86761 ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt ttggaattta
    86821 ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga gtcggtgggg
    86881 gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta gcaatcgttg
    86941 ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta aacgtcagcg
    87001 gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat cgcgatgtca
    87061 tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt tgtgatagaa
    87121 gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa atttttgtga
    87181 aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag gttgtgaaaa
    87241 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttacgt
    87301 ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt gtctcacacc
    87361 cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac taaatatgtg
    87421 aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc aaaagcaact
    87481 gcgaaaagat tacctttata tttacgctac ttgaagatgc tggatgactc aggtataagt
    87541 agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac gatccgcaga
    87601 gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt cccttttctt
    87661 atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc attagtagga
    87721 tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga aaatctaaat
    87781 atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga tggagaaatg
    87841 atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac agtagcaatc
    87901 tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa aggaaatgta
    87961 accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt caatgttcaa
    88021 tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa ttactcagaa
    88081 gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg tcgtaattct
    88141 ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt tgactaaaaa
    88201 aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg cacattcatt
    88261 cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg
    88321 agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc acttgagagg
    88381 caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt cctagaatac
    88441 gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg gaagaaaata
    88501 acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag cattggttag
    88561 aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca atcgtaatcc
    88621 cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg accttgcaag
    88681 atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg cctggtatgg
    88741 accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag caagggattt
    88801 ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt
    88861 atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat tacagtaggg
    88921 aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt gttaccttat
    88981 acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct caagcacgaa
    89041 cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt tatcatatga
    89101 gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt ccagaaacga
    89161 tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa gcgctcatcg
    89221 gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca gatgaatatg
    89281 tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat ccaaacgact
    89341 ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt tatggatgat gatggtacga
    89401 tgaatgaact agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg
    89461 tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg
    89521 gtcattccga gcggacaggt gtcgttgttg aacctcggct gtctactcaa tggtttgtaa
    89581 aaatggcgcc gttagcgaaa aaagccattg ataaccaaga aacagatcaa gcagtggaat
    89641 tcttccctcc aagattcaat caaacgtttt tacgttggat ggaaaatgtc catgattggg
    89701 taatctcacg acaattatgg tggggacatc aaattcctgc atggtatcac aaagaaacag
    89761 gtgaaatgta tgtagggatg gaagcaccag cggatagtga aaactgggta caagatcctg
    89821 atgttttaga cacttggttt agttcagctt tgtggccttt ctcaacaatg ggctggccag
    89881 atacggaaag cgaagattat aagcgatact tccctactag cactttagta accggttatg
    89941 acatcatttt cttctgggta agcagaatga ttttccaaag cttggaattt actggcgaac
    90001 gtccatttga gaatgtcttg attcatggat tgattcgtga cgaacaaggg cggaagatga
    90061 gtaaatcatt aggtaatgga atcgatccga tggaagtaat cgaaaagtat ggagcagatg
    90121 ctctccgctg gttcttatcg aatggttctg caccaggaca agatgtccgc ttcagctatg
    90181 aaaaaatgga tgcttcttgg aactttatca acaaaatctg gaatgcttca cgtttcgtta
    90241 tcatgaatat cgaaggaatg accgtagaag agatcgattt atctggagaa aaatcagtgg
    90301 ctgaccggtg gatccttact cgtttgaatg aaacgatcga acgagtaact gagctatttg
    90361 atcgttttga atttggtgaa gcaggtcgac aactgtataa ctttatctgg gatgattttt
    90421 gtgactggta catcgaaatg agtaaagaag tactttatgg agatgatgct gtttcaaaac
    90481 aaaccacccg tagcatctta gtccatacac ttgatcagat cctacgttta ttgcatccaa
    90541 tcatgccatt cgttacagaa gaaatatggg aacatattcc tcatcaggga aattctctag
    90601 tagtggcaga atatcctgtt gtacgtccag agtttgatga tgaaacagct tctaaaggga
    90661 tggaagtatt gaaagaattg attcgagccg ttcgaaatat tcgttcagaa gtgaatacac
    90721 cattgtctaa accaattacg ttgctaatta aaacaaacga tgctaaaatc gatcaatttt
    90781 tagttgaaaa tacaagctat atcgaacgct tctgtaatcc cgaagagttg acgatatcaa
    90841 gtgagatcac tgcgccagat ctagcgatgt cagctgttct gacaggtgca gaaatcttct
    90901 tgccgttagc aggattgatc aatattgaag aagaaatcaa acgattagaa aaagaactgg
    90961 aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt
    91021 ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat tatttagaga
    91081 agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa
    91141 agagagtgtg aaatcagcta gctattacta aaagctagct gattgaacat cgttacagga
    91201 aagagtttga gcctcactct tagcttcgat tcataaggag atgtgacata aattttgtcg
    91261 catctctttt ttagtctact cttattatgt aggagaagga gggaggtgga tcatgtatca
    91321 agagtcaata aagtagttga taaaatcgtt cctctctctg tttcttttct ctttgtcata
    91381 cttttatttt tcttatgtgt tctatttgtt ataataaaag agaaatgggg gaattgttga
    91441 tgacgattga agaagcaata gaatggatcc atagtcgatt accatttggt tcacgtcctg
    91501 gccttgaccg agtcgaagct ttattagaag tggtagggca tccagagaga aaagtaaaaa
    91561 cgatccatat cgctggaaca aacggaaaag gttctactgt agcttatttg agaagcctat
    91621 tagaagaggc tgggctgaca gtaggtacat ttacttcacc ttatatcgaa acgttcaatg
    91681 aacggatcgc aatcaatggc aaaagcattc cagacaatga tttgatccag cttgtaaaaa
    91741 aatatcagcc actgatcaaa caacttgatc aatttgaagc agtagctggt attactgaat
    91801 tcgagacatt aacagcaatg gcatttgatt attttttgat gaagaaagtg gacgtagcga
    91861 ttatcgaagt tggcttaggc ggcttattgg attctaccaa tgtagctgta ccaatgctga
    91921 cgggaataac aacgattgga cttgatcata ccgaaatact tggagataca attgaagaaa
    91981 tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc agtagtaaca ggaaatattg
    92041 ccccagaagc ttttactgtc atccaacaaa cagctaaagc aaaaaatgca ccgctttatg
    92101 tatacgggga aaattataaa ataaactatc tgcatcctga tgagatgtgg ggagaagtat
    92161 ttgaattttt taatgaaata ggaaagatcc ctcaattaac cactccgctt ttagggcaac
    92221 atcaagctga aaatgcagga gtagccatcg aattgtattt tgtttactgt caattggaaa
    92281 agctgccttt ccgagaaaaa gatgttttga agggattgaa gaaatctcga tggccaggaa
    92341 gaatggagcg tttgagtaac gagccattag ttgttttaga tggtgcccat aatacgcatg
    92401 caatgaaccg gcttgttgag aatttgaaaa aagaattcaa aggatatcaa atcaacattt
    92461 tgttttccgc cttacgaact aaaaatgtta gagaaatgct agaacgattg cagcaagtac
    92521 caaatgtcca tatttatttg acgacctttg aacatccaaa agccatcgaa ttaagtgaag
    92581 tatcagattt agcaagtgag agagtatcaa tcgtttcatt gtggcaattc ggattagcag
    92641 atatattgga aaagatgaca aatgaagacg tattagttgt gaccggttca ctttatttca
    92701 tttcagaagt tcgccattta ttaatacaat taggaggaga tcatgaagta taatgcagtg
    92761 atttttgaca tggatggttt gctgttcgac acagagttag tgtattacga agccacacaa
    92821 gtagtagcag acaagatggg ttttccttat gataaagacc tgtatttaaa attcttaggt
    92881 gtttcagatg aagaagtatg ggcgaattat catatgattt tctcggaatt tggcaaaaca
    92941 agtgtacaaa gattcataga tgattcatac caggaaacgc ttgaacgttt ttcgcaagga
    93001 gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga acatcagatt
    93061 cccaaagttg tcgcttccag taaccaacga gcagtcattg aacttttatt gaagaagaac
    93121 aatctgtttg aacgatttga gacgattgtt tctgcagaaa atgttcaacg agcaaaacca
    93181 gacccagaaa ttttcttgtt agctaatcag aagctaggta cagcgaagaa agaaacattg
    93241 attttagaag attcacaaaa tggtatttta gcagcttctg cggctgagat cccggtagtc
    93301 atggtaccag atctgcttcc accatcccaa gaattgatca aaaaaacagc tggggttgtt
    93361 tcttctttac atgaaatccc tgcattgtta aaataaaaat ccatctcttt tcgtatctta
    93421 ttttagataa gatagaaagg agatgttttt ttgggaaaac gattgatcaa agaagtacct
    93481 actagctctt taccaaggga acgtatggaa atttatggcg cggaagcttt atcggatcag
    93541 gagcttttgg caatcttact gcgtaccggt caacacccct atagtgtgat gtcgattgct
    93601 ggaaatctgt taaaaacgtt tggaggactg gcctctttac gacaggctac tttgcatgag
    93661 ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa
    93721 ttaggtcgac gaattcagac ggatcatgaa agagcggctc caccggttcg taccagctac
    93781 gaattggccc agcaattgat tctggaaatg aaagatcaca aacaagaaca tttggtttgt
    93841 atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg
    93901 aatcagtcag tcgctcatcc tagagaaatt ttccattatg ctgtacgtta ttgtgcagca
    93961 aggatcgttt tagcgcataa tcatccttcc ggaatatata agttagtaca cataaattat
    94021 ataaggaaat ttactgttat attgccatct ggaaatacta ttatgttatc gataattgaa
    94081 cgccataatg catttttttc ttcttccgaa gattctttat aaatttcatc gaattctttt
    94141 gatagaataa attcgacatt ttttatatct attttagctg gctctacttc aatttcattt
    94201 aattctttca ttaaagcttc tcgatctttt ttgtattcct ctaagtctat aacttcattc
    94261 aagtaggctt tcttcaatct atctaattta ttctcaatat ttttcttact atttttcttt
    94321 ttaataatct ttggttgttt ttttgaatag tttatttgta tttgttcaaa tttttgttta
    94381 gtagcttcaa aaatataacg ttctaaattt ttttcgtaaa aagcgcgctt atttgtgcaa
    94441 cgtaatttat tatttcttcg ttttacacat ctataacaag tcctttcgta tatagattct
    94501 gtaccgtctt ttttcttata tcttgcttta atagtttgac cagctactga tccctgacag
    94561 tcagaacatt ttaataaacc tttaaaaatg taatcacgtt ttgcatttgt ttttaaattt
    94621 ttaggtaagg ctttttgaac agcataaaaa agttttttat caataatagg aggacagtaa
    94681 tttttattat ttcttaattc accaatgtat tttttgttcg tcaacatatt tttaacactt
    94741 tgataatctc taacgattcc atactgagag cctaaataat aaacagtttt tcttaaacta
    94801 ccactagaaa gaaagtagtt gaatatatct atcacgatag gagcatcatc atttattact
    94861 aatttcttat tttctataga gtagcctaac ggagttttcc cgcttactac ttcaccttgt
    94921 gcaattttat ttgccataac agctttgatg cgttctgaat caatttgggc ttccaattca
    94981 gcaaaactca ttacttgtgc aatgaaagtt ctgccatacg ctgtagttgt atcataatat
    95041 tgttgagaaa cagcattcca agaaacattg tgtttttcta aaatttcttg agtgtttaaa
    95101 taatgtcgta gatttctaaa ccatctatct aattttgtaa ataaaattag atctatttta
    95161 ttttctttta catcttgtaa tagtttttga aattcatcac gatttatttt ttgaccagaa
    95221 ataccaccgt ctacatattc attaactact ttcaaatcct tgtgttgttc gatatattca
    95281 tagagggttt ctttttgttc tctaagacta tctccatgtt tggcttgttg ttcagttgat
    95341 actctcatat ataatgctac tcgtttcatg ttatctcctt tcgaatgtat gttcttatcg
    95401 ttgtaaaaga aaagcccgaa ggctaatctt tttactacac atctgttaat tctaatgatc
    95461 ttctgtattg ttcagcttga gctagtttag taaaagatac agttccgtta taattttcta
    95521 aaacaacttt ttcaatttca tcaagagata ctttaaagaa ttcttttcgt tgattaactt
    95581 tattgacttc gttttttcta aaatgcgaat gtagttgatt ttctaatgtt ggtgcatctt
    95641 ctgaaaatat cattgcatga acatcaaatt caaaaggtac agaagcgcta ctaagttcct
    95701 taattctatc cataggttct aaccgtctag tcattcctat tttatagaca tcttcaccga
    95761 aagatccgat attactgatg acataaacat agccagctct agtattaagt tctctttgca
    95821 atacatcttc tttgacagtt tctagttctc ttaatttctc ttccagttct ttaattttat
    95881 cagcatagag ttctttttca acatcagcac tagctttttg catgtatgcc attaacttag
    95941 ttatctctga gttaaactgt cgttcttctt tatcaatgcg tttcttttca cgttctattt
    96001 cacgtcttac cttttcttct tcaatcattt gttcttttat tgctttttgc tgttcttttt
    96061 catcgttaac tttaactatg tatgaatagt tgagttctaa ttcttgaagt tttaattcaa
    96121 gtaattcttt tttgaattct gcttcatcgt ttttaaaaag cttgtttatt ttatcaaaag
    96181 ttcttataat tttttctcta gttgaatcta cgttagaaat agtaagttta ttgagaagtc
    96241 cagttatttc tgattctaat ggtataagca ttttcttttt aatattatta gcttctctat
    96301 tattcaaata agaaggaata ttaattgcac cgtctaaaat aaaatccttt tctttaatct
    96361 ttaataaaga aagtttgttt tttatttcgg atgaagaagt ttcatctata ttcggaataa
    96421 ttactgattt atagatagtg accagagatg tgtcatattt ttgattaatt tcatcgtcta
    96481 accgctggct ttcagcatta agaatatcaa ttttgttttc tagcgttgac aagtttttat
    96541 tcaatttact tatgtttttc tctttttcag atataaacgt ttccctagca tttaattctt
    96601 gagaatattc ctttacctta ctctctaagg cagaagtttt atcttttaat tttttagctt
    96661 cagataattt gctactaccc ataattatta aaactattcc aactaaaaac aatagtactc
    96721 ccattcaaaa cgcctcgctt cttttataaa ataattgtca aaatcatata aacccttacc
    96781 gaagttgacg gctccggcat gggaattttt ttaaaataca acttttccga taattctaat
    96841 ctgttctggc gatacaattc tatcttcgta taagtcgttt atagaacgta aaatgattgt
    96901 ttgattttcg taatctttat atatctcttt acatgtaact ccgtcaccac ctaattccac
    96961 aatagcaatt tccccatctt ctacgtccgg ttgttcttta acgaatacga attgtccatt
    97021 cctgataaca ggttccattg aatcgcctat aataggtact gcgaagtcag cagcacttgg
    97081 aactctctca aattcttcct cttcgacaac cgtatcaccg taagtaagtt ctgttggatt
    97141 ggcagccgaa gccgaattgt gaacagttgg gattttttct tttttaggaa aatctaggac
    97201 attttcttgt ttttgttctt ctaatcgatt acttgcaaat tcataggtag caactttatt
    97261 ttcgtcgtta agctgattat atataggaag gatattatat atctgattag atatagataa
    97321 atcaatattc atcaaatcat ctatatcaac attgaatatc ttagctatct catttaaggt
    97381 ttttaatttt ggtgtatatt ttcctttttc ccattcgctt attgaggatg aactttttct
    97441 tcctagtttt tcggctagtt cttgttgatc aatattatac ttttctctaa gaaactttag
    97501 attttcagcg aacatagtaa cacctccttg agttaattgt aacaccgttt cggaaaaaat
    97561 gtaacaggtt tcttagaaaa tattttagtg tttttaaaga agtatttacg caaaatagaa
    97621 atcgatttca ggaaaactga aatatgcact tgacttcgga aaaaccgaag tatataattt
    97681 agtcagaaag ttaaagagag gaggcgaata aaatatggat accgcgaaga aagaaaaata
    97741 tacattgaga caattaagag ctttaaaggg ttattcaaaa gaagaactgt caaggaaaag
    97801 caaagtgaca tcaagaacaa tatttattta cgaaaacgat gtagataaaa tgaggaacgg
    97861 aaagtacgcc actctagaaa aaattgctaa ggcattgggt gttagagttt ccgatatttt
    97921 tttagaccct gattcggaaa aaccgaaatc aataacaggt gaaacaaaac aagtcagcta
    97981 ggaggtgtga atagtggaag agaagaatat aaaagtaccg actcttgaag aatatacaaa
    98041 aattgttgtc gaaacagacg agcagaaccc gaaaacaatc gcagttatta cggcgaatga
    98101 tattgaatca tgcgaaggtt ttcgagttcg aatgacccct agatatgatt aatgttcttt
    98161 atcacgtgga ggtataggat cgtttccaaa actatctttt gattgtattt gtccattctt
    98221 tttttggaca ataaactctg attgctgttt tttagaaatc tttttaccat actcaacagc
    98281 atctgattta ttatcaaata gttttgtatc ttcggaatta ccttcaccac gtacagccca
    98341 tttgccattt ctaggagata cccattgatt tttacccata acaattcacc tcgctttcaa
    98401 gttaattcta ttaagaggtg aaaagaaaac aaactgatgg aggtgagatt gtgagtaaca
    98461 agccaaaaat cactttgaat ggcacaccta atcttcaaag attagcagat gaaatggcta
    98521 gaatacgttc tagacattgg ggagttactg tggtggcaac cataattgaa caaaaagaaa
    98581 agcccacaca gccggcaagc aagtagtggg caccatagaa caataatatt atatcacaca
    98641 ctcgccactt ccgaaaagag gagttgctgc tctcctttca agtttgccaa agtccacgtt
    98701 tcttcatttt ggcaactcct cttcttggag gtggtgacgg cgagagacga aggagggatg
    98761 acatgaagga agaatcaaaa atagggattt tgaatgagaa caattacaca agattaatta
    98821 ttgaaacaga agaaggggaa aaagtagcag aagttacatt gaccgatgca actcctgctg
    98881 acggttatcg tattcgatta actcctaaat atgattaacc ttttggtggg tgtggatcgt
    98941 ttccgtgact atcacggctt tgaatttttc catctctgcc atgaataaat agctcagatc
    99001 cttgattttt agaaatctct ctagcgattt ttactgcttc tgattttgta ttagtatgtg
    99061 cagtcgcttt agaatttcca gcacctttga cattccaacc accattggaa ttaggtacaa
    99121 catgttgatt ttttcccata gttttcacct ccatttctat agaaatattt tatcaaatag
    99181 aaatggagaa attcaataaa cgaaaggagg gatcaaatga cattacaaga gcatcaacac
    99241 ttgatgcgaa aactaaatca ggaataccac gaggaaatga aacctagatt gctaggggaa
    99301 caatggcgtg aacgccagaa gaagtggtta gaaataaaaa agtgactcag ccgtccaaag
    99361 caatgagtca caaaccaaaa attataccta aggagatttt atcacatgaa taagaaaatt
    99421 gaaaatctta tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt
    99481 acagcccaaa aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta
    99541 ctttgtcttg ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt
    99601 atgcgtagaa ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct
    99661 tcaaatcata cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt
    99721 ctcaaggggg aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt
    99781 tcacgttagt gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca
    99841 tgcttgtgct ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat
    99901 cttgacaact atttaactga tcctgattat gctaaaccac catatgaagc gccaattgat
    99961 gaggaggatg aagatgagta aatctacctt agaaatgagc catcaagaat ggctcgaaga
   100021 ccgcaaaaga ggtattggtg gctcagatgt tgcaaccgta cttggattaa acaaatacaa
   100081 atctccttat caattgtggc ttgagaaaac gggtcaaatt gaattgaaag atttagaaag
   100141 cgaaccagct tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg
   100201 tacaggcaaa aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt
   100261 aagagcaaat attgatcggg aagtagtggg agaaaatgcc attctggaat gtaaaacagc
   100321 caatcaattt ctcggaaaag agtgggaagg tgaagaggta ccgcttagct atctctgtca
   100381 agttcagcat tacatgaacg ttttaaacaa agactattgt tatatcgctg ttttgatcgg
   100441 tggacaaaaa ttcatttgga agcggattga acgagatcaa gagctgatcg atacaatcac
   100501 tgaacaatta gtagagtttt gggaaacgaa cgttcttgga ggtatcgagc ctgtaattga
   100561 cggaagtgga gcgactgctg acttcttaaa agaaaaatat gcagatgtag aagaaaatca
   100621 aacagctcta ccatcacgtt ttgatgaact tatcgagcaa aaaagagaac tcaagcggac
   100681 gaaaaaagaa attgaatcat ctattcgtca agtagacaac gagatcatca gtgagctagg
   100741 taaacgtgaa gccagtatcg gtatcacaca aagaaacatt atcagctgga aacttgttcg
   100801 tacaagacgg atgaacacga agaaactagc agagaaatat ccagatgtcg caaatgatga
   100861 agagatttat aacgttactg aatcaagaag gctaaccgaa aaggagatca aataatatgg
   100921 caacaaatga atcgttaaaa aatcaattgg cagaaaagcc acagaaacaa gttgcaccag
   100981 gacagttagg gcttaaagct ctaatgaata caccaacaat gagaaagaaa tttgaagaag
   101041 tacttcatga caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca
   101101 gttatcttgc agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat
   101161 taaatcttgg gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa
   101221 acaagcaaac aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata
   101281 tccaattagc ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag
   101341 gagaactaaa aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa
   101401 cgtctgatga agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa
   101461 ctgtctattg gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg
   101521 ataagacaaa gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg
   101581 ttttgagaaa tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca
   101641 catcggatga gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag
   101701 ttgaagaaga tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg
   101761 tacaaactgg attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc
   101821 tcccttgctt ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa
   101881 aagtgatgaa ttcattcgtt tggaccaatc cttatatgta taaattatgg agcttgtgtt
   101941 tgatgaaagc gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga
   102001 acagcggaga attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga
   102061 aacgtgaaca tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa
   102121 acggaatgtt gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt
   102181 ctttgtacca aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag
   102241 tgcacacaaa caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt
   102301 cgcaaccacg caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg
   102361 agttattcaa acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg
   102421 cagataacat tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa
   102481 tgattgaatg gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat
   102541 tgcgagaaaa atacgacaca atggcagcgc aagcaaatcg tgattataaa acaaaacaaa
   102601 ctaaaacgct tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt
   102661 aggcatggaa actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg
   102721 tggcgaatgt cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg
   102781 ctcagaacgt tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga
   102841 agacttacaa acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt
   102901 caaaggtggt tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca
   102961 aattgtcgat caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt
   103021 cttgttagga aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca
   103081 cctagcgatg gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt
   103141 atttatcggg ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact
   103201 cagaaaaacg attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat
   103261 tgatgatatc ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa
   103321 cacgctaaat tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt
   103381 gcttggtgaa gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa
   103441 ctctgatgga tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa
   103501 aggagcgatc gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg
   103561 caaattatag gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg
   103621 aagataagta agctaatcgg tgcagatacg atggatcgat ccaagccatt acccaaactg
   103681 aattttgaat catttacgtt tgagaaatat agccatctga gagaggtagg ctatagtgta
   103741 aatgctattc gaaaagcact tggggtagga agtagtgtat gggcaaaatg gcggaaagaa
   103801 aatgtaaatg aggtaaatca aaatgattaa gctaataggc aaagagacgc tcaaggttta
   103861 ctatgtagcg gaaacaaagg cagaagtcag ccgttggcta ttgaaaatgt ttaataagac
   103921 tagagccaat gcttcagtaa gtcagaaagc aagtgtatca gaaacaggca tgcctgaagc
   103981 aatgtggatt tcaaggagga actgagatgg caatgatacc gaagtttaga gcgtgggata
   104041 aacgaaagaa cgtaatgaga gatgtagccg tcttgcattt tactaaaaac ggcaaaacaa
   104101 actttattga atattggata aatcctaccg aattgaaatc atatcacgtg cgaaacatcg
   104161 acctcatgca atccacagga atgaaagata agaatggtgt ggatatattt gaaggggata
   104221 tagtattagt cagcgtgcga aatggcttcg attacttaga taataaagtc tgtattgtca
   104281 aaaattcaat agattattcc ggattagttt gtgccactgt tgatgaagac ttagagtatc
   104341 aaatttttaa cacagagctg tttgaagaat acacgtacga agtcaccgga aatatatacg
   104401 agaatagcga gttattggag gaacagcgat gaataaacag gaagcaatca aaaagttaga
   104461 aagtattaaa gcgataggaa atgatgcaat agctgcttgc tataacgaga gtataaattc
   104521 aggtattacg ttaatgaaaa aaatagacga accgcagaaa ccagttattc cacaactcgt
   104581 ggccggttgg cttgagaaat ctacggaccc ttt
//
LOCUS       DACEDM010000002        82058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEDM010000002 DACEDM010000000
VERSION     DACEDM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.002"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IWO50_000094"
     CDS             complement(218..1015)
                     /locus_tag="IWO50_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0357252.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IWO50_000095"
     CDS             complement(1012..2196)
                     /locus_tag="IWO50_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0357253.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IWO50_000096"
     CDS             complement(2221..3468)
                     /locus_tag="IWO50_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0357254.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IWO50_000097"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IWO50_000097"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0357255.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IWO50_000098"
     CDS             5448..5762
                     /locus_tag="IWO50_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0357256.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IWO50_000099"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IWO50_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0357257.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IWO50_000100"
     CDS             6447..7091
                     /locus_tag="IWO50_000100"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0357258.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IWO50_000101"
     CDS             7106..7435
                     /locus_tag="IWO50_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357259.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IWO50_000102"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IWO50_000102"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0357260.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IWO50_000103"
     CDS             8423..9247
                     /locus_tag="IWO50_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0357261.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IWO50_000104"
     CDS             9240..9587
                     /locus_tag="IWO50_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0357262.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IWO50_000105"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IWO50_000105"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0357263.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IWO50_000106"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IWO50_000106"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0357264.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWO50_000107"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWO50_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0357265.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWO50_000108"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWO50_000108"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0357266.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IWO50_000109"
     CDS             15244..16011
                     /locus_tag="IWO50_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357267.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IWO50_000110"
     CDS             16001..18043
                     /locus_tag="IWO50_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0357268.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IWO50_000111"
     CDS             18324..18473
                     /locus_tag="IWO50_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0357269.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IWO50_000112"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IWO50_000112"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0357270.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IWO50_000113"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IWO50_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0357271.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IWO50_000114"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IWO50_000114"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0357272.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IWO50_000115"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IWO50_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0357273.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IWO50_000116"
     CDS             22890..23972
                     /locus_tag="IWO50_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0357274.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IWO50_000117"
     CDS             23972..24775
                     /locus_tag="IWO50_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0357275.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IWO50_000118"
     CDS             24845..25585
                     /locus_tag="IWO50_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0357276.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IWO50_000119"
     CDS             25596..26600
                     /locus_tag="IWO50_000119"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0357277.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="IWO50_000120"
     CDS             complement(26645..28489)
                     /locus_tag="IWO50_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357278.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28692..28994
                     /locus_tag="IWO50_000121"
                     /pseudo
     CDS             28692..28994
                     /locus_tag="IWO50_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            29242..29460
                     /locus_tag="IWO50_000122"
                     /pseudo
     CDS             29242..29460
                     /locus_tag="IWO50_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(29589..30797)
                     /locus_tag="IWO50_000123"
     CDS             complement(29589..30797)
                     /locus_tag="IWO50_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HAQ0357279.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            31087..32052
                     /locus_tag="IWO50_000124"
     CDS             31087..32052
                     /locus_tag="IWO50_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0357280.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            32074..32286
                     /locus_tag="IWO50_000125"
     CDS             32074..32286
                     /locus_tag="IWO50_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0357281.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            32361..32837
                     /locus_tag="IWO50_000126"
     CDS             32361..32837
                     /locus_tag="IWO50_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ0357282.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            32834..33334
                     /locus_tag="IWO50_000127"
     CDS             32834..33334
                     /locus_tag="IWO50_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HAQ0357283.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            33894..35711
                     /locus_tag="IWO50_000128"
     CDS             33894..35711
                     /locus_tag="IWO50_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0357284.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            35820..37145
                     /locus_tag="IWO50_000129"
     CDS             35820..37145
                     /locus_tag="IWO50_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0357285.1"
                     /translation="MQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTIN
                     FSKSMKYNPFVYIHSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIW
                     YEAPAEEMNFSTLLEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLL
                     SAGKTRSSILISCGARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFN
                     FILAMLQSQLINLLCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREI
                     SASIILQSQSQLKAIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTS
                     ENRGSQTSHGLNYQKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYK
                     YLADADKKNTFDVDRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            37222..37383
                     /locus_tag="IWO50_000130"
     CDS             37222..37383
                     /locus_tag="IWO50_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357286.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            37424..37804
                     /locus_tag="IWO50_000131"
     CDS             37424..37804
                     /locus_tag="IWO50_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HAQ0357287.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            37819..38100
                     /locus_tag="IWO50_000132"
     CDS             37819..38100
                     /locus_tag="IWO50_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357288.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            38359..39141
                     /locus_tag="IWO50_000133"
     CDS             38359..39141
                     /locus_tag="IWO50_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAQ0357289.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            39314..41032
                     /locus_tag="IWO50_000134"
     CDS             39314..41032
                     /locus_tag="IWO50_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAQ0357290.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            41034..41888
                     /locus_tag="IWO50_000135"
     CDS             41034..41888
                     /locus_tag="IWO50_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357291.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            42564..42884
                     /locus_tag="IWO50_000136"
     CDS             42564..42884
                     /locus_tag="IWO50_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357292.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            42908..43318
                     /locus_tag="IWO50_000137"
     CDS             42908..43318
                     /locus_tag="IWO50_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0357293.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            43465..43671
                     /locus_tag="IWO50_000138"
     CDS             43465..43671
                     /locus_tag="IWO50_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0357294.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            44018..44539
                     /locus_tag="IWO50_000139"
     CDS             44018..44539
                     /locus_tag="IWO50_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0357295.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            44597..45022
                     /locus_tag="IWO50_000140"
     CDS             44597..45022
                     /locus_tag="IWO50_000140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0357296.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            45201..45431
                     /locus_tag="IWO50_000141"
     CDS             45201..45431
                     /locus_tag="IWO50_000141"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HAQ0357297.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            45465..45647
                     /locus_tag="IWO50_000142"
     CDS             45465..45647
                     /locus_tag="IWO50_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HAQ0357298.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            45760..47271
                     /locus_tag="IWO50_000143"
     CDS             45760..47271
                     /locus_tag="IWO50_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357299.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            47594..47818
                     /locus_tag="IWO50_000144"
     CDS             47594..47818
                     /locus_tag="IWO50_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HAQ0357300.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            47811..48377
                     /locus_tag="IWO50_000145"
     CDS             47811..48377
                     /locus_tag="IWO50_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HAQ0357301.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            48365..48595
                     /locus_tag="IWO50_000146"
     CDS             48365..48595
                     /locus_tag="IWO50_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357302.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            48796..49161
                     /locus_tag="IWO50_000147"
     CDS             48796..49161
                     /locus_tag="IWO50_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HAQ0357303.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            49161..50888
                     /locus_tag="IWO50_000148"
     CDS             49161..50888
                     /locus_tag="IWO50_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAQ0357304.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            50918..52834
                     /locus_tag="IWO50_000149"
     CDS             50918..52834
                     /locus_tag="IWO50_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0357305.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            53519..53701
                     /locus_tag="IWO50_000150"
     CDS             53519..53701
                     /locus_tag="IWO50_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357306.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            53728..55425
                     /locus_tag="IWO50_000151"
     CDS             53728..55425
                     /locus_tag="IWO50_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0357307.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            55450..55581
                     /locus_tag="IWO50_000152"
     CDS             55450..55581
                     /locus_tag="IWO50_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357308.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            55673..56536
                     /locus_tag="IWO50_000153"
     CDS             55673..56536
                     /locus_tag="IWO50_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357309.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            56567..57109
                     /locus_tag="IWO50_000154"
     CDS             56567..57109
                     /locus_tag="IWO50_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAQ0357310.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            57123..57545
                     /locus_tag="IWO50_000155"
     CDS             57123..57545
                     /locus_tag="IWO50_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ0357311.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            57475..59874
                     /locus_tag="IWO50_000156"
     CDS             57475..59874
                     /locus_tag="IWO50_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ0357312.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            59906..61897
                     /locus_tag="IWO50_000157"
     CDS             59906..61897
                     /locus_tag="IWO50_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0357313.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            61920..62171
                     /locus_tag="IWO50_000158"
     CDS             61920..62171
                     /locus_tag="IWO50_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAQ0357314.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            62161..63390
                     /locus_tag="IWO50_000159"
     CDS             62161..63390
                     /locus_tag="IWO50_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAQ0357315.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            63387..65468
                     /gene="topB"
                     /locus_tag="IWO50_000160"
     CDS             63387..65468
                     /gene="topB"
                     /locus_tag="IWO50_000160"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0357316.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            65617..69537
                     /locus_tag="IWO50_000161"
     CDS             65617..69537
                     /locus_tag="IWO50_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAQ0357317.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            69538..70482
                     /locus_tag="IWO50_000162"
     CDS             69538..70482
                     /locus_tag="IWO50_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357318.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            70486..70689
                     /locus_tag="IWO50_000163"
     CDS             70486..70689
                     /locus_tag="IWO50_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357319.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(70989..71225)
                     /locus_tag="IWO50_000164"
     CDS             complement(70989..71225)
                     /locus_tag="IWO50_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0357320.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(71408..71779)
                     /locus_tag="IWO50_000165"
     CDS             complement(71408..71779)
                     /locus_tag="IWO50_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357321.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(71776..73104)
                     /locus_tag="IWO50_000166"
     CDS             complement(71776..73104)
                     /locus_tag="IWO50_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0357322.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(73065..73394)
                     /locus_tag="IWO50_000167"
     CDS             complement(73065..73394)
                     /locus_tag="IWO50_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0357323.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(73652..74023)
                     /locus_tag="IWO50_000168"
     CDS             complement(73652..74023)
                     /locus_tag="IWO50_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0357324.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            74168..74353
                     /locus_tag="IWO50_000169"
     CDS             74168..74353
                     /locus_tag="IWO50_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0357325.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            75296..75955
                     /locus_tag="IWO50_000170"
     CDS             75296..75955
                     /locus_tag="IWO50_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAQ0357326.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            75958..77301
                     /gene="vanS"
                     /locus_tag="IWO50_000171"
     CDS             75958..77301
                     /gene="vanS"
                     /locus_tag="IWO50_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0357327.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFP
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            77472..78278
                     /gene="vanY"
                     /locus_tag="IWO50_000172"
     CDS             77472..78278
                     /gene="vanY"
                     /locus_tag="IWO50_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0357328.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            78296..79123
                     /gene="vanW"
                     /locus_tag="IWO50_000173"
     CDS             78296..79123
                     /gene="vanW"
                     /locus_tag="IWO50_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAQ0357329.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            79120..80091
                     /locus_tag="IWO50_000174"
     CDS             79120..80091
                     /locus_tag="IWO50_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0357330.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            80084..81112
                     /gene="vanB"
                     /locus_tag="IWO50_000175"
     CDS             80084..81112
                     /gene="vanB"
                     /locus_tag="IWO50_000175"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0357331.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            81118..81726
                     /locus_tag="IWO50_000176"
     CDS             81118..81726
                     /locus_tag="IWO50_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ0357332.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT        24367 a        16920 c        19854 g        20917 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    28561 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    28621 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    28681 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    28741 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    28801 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    28861 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    28921 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    28981 atttttcctc aaaattttag gttatcgctg aaagccccgg aaatacgggc atttcgcagg
    29041 atatgagaac tcaatttact accaatttac tactttcgga ttgagccgca tatatgctga
    29101 accatactgc taatacccgc caccccaaat acggcacatc ataaattgaa tacgacaccg
    29161 taaacgcggc gttcctcttt cccgtcatgg gagaacagga cgccgctttt ttatgcccaa
    29221 atagagaaag gaggcgacgc catgctattt acttccggcc ctgaccgggc gtttgaacgg
    29281 ctgatgcagg aaaagcccgg tttcgatcac tacgacggcg gcggggcgga cgcgccggag
    29341 gattgcggca cctgccgatt ttaccgcccg gattggaaat atcaattctg ccagtttgca
    29401 gagtgtccct atcagccggg caagctgacc gactatgcag cggtcaaatt cacagtgaaa
    29461 gacctgctaa taaaagtcaa gtagaaattt cagattttta gggaggcgaa aaaatgcaat
    29521 acaaaatcaa gccgctgagc atctcaaaat taaaacggcg gccagccaga tggtatagag
    29581 tgtaaaaatc agtcgtgctc cagggattcc aagtaggctt tcacagaagc gtagtagatg
    29641 cgcaggaact tgttggcaga ggccatcatg tagacacgat atggcttgcc ttcagagcgt
    29701 ttcttgtcca tgaactggta caccggctcg tctattggag cacattgcag gatgacgctc
    29761 atcaccagaa agagcgtcct gcgcagtgag gcagatcctc gcttggaaat gctacggcta
    29821 cggacatcta tttgaccgga ttggtagggc ggggcgtcaa tacccgcaaa ggcaaccaac
    29881 gcttttttgg aatgaaaacg gcgtacatcg ccaatttcag ccaggagttg agggccgagg
    29941 gttgggccaa caccaaacat ccccatcact acaggatact ccggcagaga agctgccagg
    30001 gattgcatct cctgtttgag agcagctaac gcggaagaag ttgtctggag ttgagaaatg
    30061 gcctgttcta ccaaaagttt tgccgtatcc gttttcggta tgacaccgaa gtgtctgcaa
    30121 gcagaggcat aaatatccag cgccttatcc tcgctgaaat tgtagccgtg ctttctgcac
    30181 cacttctggt attgggtagt aaaggccttc tcagaccggc cacaaacgca ctcgcaatgc
    30241 caaaaagtag cgacaaagtc cacccacttc tcgctgccat cggcgcgggg cggactggta
    30301 aacaagcgat ttgcgtctgg gaaggtggtg tccagcaggg agatcaggtt attcttcagc
    30361 atggtctgaa ctttggaata ctgttggtac tgccggtagc agattttcag catgagtcgg
    30421 gtatcctcct ccgggatata tctcggcaat gtgagccagt ggtcaagacc gtagttggcc
    30481 agctttacgg catccttttt gtcggttttg gcccgtctta aactgttgtt tccgtagtca
    30541 tgcaccagca ttgcattgac taccgagaca taaaggcccg cgtcgtggag cagccaggcc
    30601 accggagcat ggtaattacc cgtggattcc atcaccacac gggtctcgcc gtccaggctt
    30661 ttgagcagtc ctgccagctc gctcagttca ttggcggtgt gacgtacttc aaagggtgaa
    30721 accactacct ctccgaaggg ccgcatgact gcaatcatgc tcttaccctt ggaaacatcg
    30781 atgccaacgc agttcattca cattcctcca atacaaagaa tctgcaaccg gaatccatct
    30841 tttctcgctg ccgattcaat ctattgtgtg acacgaactc tccgacatcc ggtggttcaa
    30901 cctgcttaaa tcgaacgctg caacgagagg atggctaaca gtctttcaca cggacataca
    30961 agcccaagga gtgattggtc agccagttgc tcctctcatt gtagcttagg cacgagatgg
    31021 agggaaagcc ggactggctg tccggctttc ctgtccaaat ttattgtaat aggagcgaac
    31081 aacgctatgg cagttttccg tgtggaaaag aacaaaggct atacggtgat gtcaaatcac
    31141 cacctgcgga ataagaacct gaccttaaag gccaagggcc tgctgtcgca gatgttgtcc
    31201 ttgccggagg attgggacta taccctaaaa ggactgtcct atatcaaccg ggaaagcatc
    31261 gacgcgatca ggacagcggt atgggagctt gaaaaagctg gatatatccg gcgtgagcag
    31321 ggccgtgacg caaaaggcaa aatggccgat atggtctata ccatctacga acagccggta
    31381 ttggaaaacc cggtattgga aaatccaaca tcggataacc cggtgttgga aaatccgaca
    31441 tcggataatc cggcgtcgga aaatccaacg caattaaata aagaagtaca aagaactgac
    31501 ttatcaaaaa aagaaaaaat aattacagat ggacaaagta cccattccat tcctatccca
    31561 tccccgaccc ctgcccctat ggagggcgag gcagcggcta cgccgccgga acggaaaagg
    31621 acaggaaagg atgaggctgt acggatttat cgggaaatca ttttggagaa catcgagtat
    31681 gcctatctca ttcaggacag gagcattgac cgtgaacagc ttgacgaaat cgtagacctg
    31741 atgctggaaa ccgtctgcac cgcccgaaag acaatccgtg tcgccgggga cgactaccct
    31801 gccgagctgg tgaaatccaa gtacatgaag ctgaccggcg aacatatccg ctttgtcctt
    31861 gactgtctgc gggagaacac aacgaaagtc cgcaatatca aacaatacct gcgggctatg
    31921 ctgttcaacg cccccagcac catttccaac tactacacgg cccttgtcgc gcacgacatg
    31981 gcacagcccg attggggaaa gcctaaatcc ggcctgccgg actattcctg cggcgagggc
    32041 gaaagcctgt aacgagaaaa ggaggacgac cctatgaagc agggcgcatt gatttttgac
    32101 gagcgaacag accgttacga catccgcttt gatctggccg actattacgg cggcctgcat
    32161 tgcggggagt gtttcgacgt gatgatcggc ggcaaatgga ggcccacccg cattgaaatg
    32221 gccgaaaact ggtatcttgt cggcatccgg gccgacgacc tttccggcct gcgggtgcgg
    32281 atttgaccta tatctacctg cggcgtctgg tggttccgcc ttagacgccg cttttgactt
    32341 tctgacaagg agggatgcga ttgcaggaag aagttactca aaagaccatt gccctgtcca
    32401 tgaaaacggg caagctcacg gctcaagtgc tgcaagcggc gctgaaaaag tacctgcaac
    32461 accgggccaa gggcaaaaac acgctgcacc acggccagca gagcctaaag cagcttaaaa
    32521 agcatggggc tgatctctcc aacattgaga tcaccgaggc caacattgga gcgttcaagc
    32581 catgcgccaa gaaatacggc gtggatttta ccttacgaaa agataagacc acccagccgc
    32641 cccactacgt tgtgattttc aaggccaagg atgcagataa ccttgatctg gcgtttcggg
    32701 agtttaccgc caagacgctt tccaaggagc agcggccctc tatccgcaag gttttgtccg
    32761 ctgtgaagca aaaggcggcg gcccagacca agcagcgggc caaggagaaa atcaaggaaa
    32821 gggggctgga actgtgaagc ctgacgtaaa gaaattgctg atcctgaacc tcccgtatct
    32881 gatttttgtg tacctgtttg cgaaatgcgg ggaggcgtac cggctggccg ctggtgctga
    32941 cctgtcgggg aagctcctgc attttgcgga tggttttgcg gcggcttttg ccaacccgct
    33001 gccaagcctg cacctgtttg acctgtgtat cggtatcgcc ggtgccgtga tcgtgcggct
    33061 ggtggtgtac tgcaagagca agaacgtgaa gaaataccgc aaaggcatgg agtatggcag
    33121 cgcccgctgg gggacggcca aagacatagc gccttacatc gaccccaagt ttgaaaataa
    33181 tatcctgctc acccaaacgg aacggcttac catgaccggc aggcccaaag accccaagac
    33241 agcgcggaat aaaaacgtgc tggtgatcgg tggcagcggt tcagggaaaa cgagattttt
    33301 cgtgaaaccc agtgcgcccg taagggcggt ataaatccta ccttgagcaa gtagtaggta
    33361 aaagtatcaa gcggcattgt gccgcaacct atcattcccc gtcttacccc aaaagggaaa
    33421 ccggagggcg accatagcat gacggaggac acggggcgct gaagcctcag aagcgccggc
    33481 gtgacggaat gaaaatccac gtatgaggat ggaagctaga ctgcttgaat gacagcctga
    33541 aatcgggacc aagagcgtac ccctgacgta gagaggttgt actcgtcagt gttcctgaca
    33601 gtcgcattgt ttggctatgc ggctgctgat actcggagca ggttcagcca cgggaaagtg
    33661 gaaaaaggcg aacgtcacat accgacaacg tggaacgctt gtttataccg tactaaacgg
    33721 ggattgccta aagtgaaatg ccgaaaggct atgaaaacgc tgaaatgcgg ggttctgaat
    33781 attcaccatg gcaacagagt tcccatagta gtctgcggac gggaaagccg tctacatggc
    33841 aaaggggaac agtgaatcat tttcaaaaca gaaaggatgg tgtgtgagac actatgagaa
    33901 atccgattca tgtcttgaaa agccttgaag aaaaggcaag tgtaagtaac tacaaatatg
    33961 aaagattata tcgcaaccta tacaatccag agttctatct ccttgcatat gcgaacattg
    34021 cgaaatcgca ggggagcatg acgcaggggg tagacgggca gacgctggac aacatgagcc
    34081 tgccgagaat taaccggatt attgaatcca tacgcaacag gacatatcag ccaaagcctg
    34141 caaaaagaaa gtacattcct aaaaagaacg ggaaacttcg cccgttggga atcacttcta
    34201 ctgatgataa gttagtgcag gaagtggtca gaatgattct tgaagcaatc tatgagccga
    34261 cattcagcaa caattcacac ggtttcagac caaaaagaag ctgtcacacg gcacttacgc
    34321 aagtgaagaa aaacttcaca ggtgttacat ggattgtcga gggagacatt aaggcgtgct
    34381 ttgataactt cgaccatcat gtgttggttg aattactgcg gaaaaggatt tcagacgagg
    34441 cttttatcgg tctaatctgg aagttcctga aagccggata tatggaacaa tggcagtaca
    34501 actgcaccta ctccggtgtt ccgcagggca gcggtatcag tccgatatgt gcaaacatct
    34561 acctcagcga actggacaac tatatgcagg aatacaaaga aaagtatgat tgcgagccag
    34621 aacgcagaag gacgaccaga gaatacgagc gggcgtcccg gagatacaga aaggcacgta
    34681 aagcgttaat gggcgcagaa aaatcaactc ctgaactggt aaaagaattt aaggattcca
    34741 gaaggaagaa gatgaatcag cactattaca atccgtttga ggaaggcttc aagaaaatcc
    34801 agtacaaccg ttacgccgat gattttgtaa tcggcgttat cggctccaaa aaggacgcag
    34861 aaaaaatcaa ggaagatgta aaaatctttc tccaagaaaa actgcatttg gaaatgtccg
    34921 aggaaaagac gaaagtcacc cattccagta agccggtacg ctatctgggg tacgatttca
    34981 aagtaatcca ttccaagaac atgaagcgtt gtaaaaacgg cgacatgaaa cgggtgtggt
    35041 atggaaaagt attcctgtat atgccgaaag aaaaatggat taaaaaagcg atggaacggg
    35101 gagcgataca ggtgaaacgc aacaatgaca caggcaaaga aatgtggcgg cctatgccaa
    35161 ggaaagacct gatgaaccgc agcgacgcag agattgtgtc tactttcaat tccgaaattc
    35221 gagggttata taacttctat cggatagcag aaaacgtagg cgcattgcac aagtattact
    35281 acatggtgcg gtacagcatg ttaaaaactc tggcaggaaa gcacagaacg aatgtcagtg
    35341 ttattaagaa gcggcacatg gtaaacggag tactgagaat cccatacgat acaactaagg
    35401 gacgtaaata ctgcgaattt taccatgatg gattcagaaa gcacagcgac ggctatgaca
    35461 atgtggcaga cgttatgccg agttatagga aatatgacag caggcacacg atagtaaacc
    35521 gcataaaagc cggagtatgc gagatttgcg gggaacatgc agactattta tgtatgcacc
    35581 acgtaagaac gctgaaatcg ctgaaaggca gggatatatt tgagcagaaa atgctgaaaa
    35641 tgagaagaaa atctctggct ctctgccctg actgctttga actcttacac gaaaccaaag
    35701 aatcaaggtg attcatggaa agccggatac gctgagaggt gtacgtccgg tttgggaggc
    35761 ggctccctga aacctactga tgaaagtcag caaggcgcag ggtgcctacc tcatacctga
    35821 tgcaatgctt tccgaccacg gactatccga cttcgtttgt ggtgacagat ccgaaaggca
    35881 ctttggttct ggaaaccggc aggatgttcc agcaggcggg ctaccgcatt aaaatcctga
    35941 ataccattaa cttttccaag tccatgaagt acaacccctt tgtctacatc cattcggaaa
    36001 aggacgtgct aaagctggta aataccctga tttccaacac caagggcgag ggggaaaagt
    36061 cagcggagga tttttgggtg aagtcggaac ggttgtttta ttcggccctg atcggctata
    36121 tctggtacga ggccccagcg gaggaaatga acttctcaac gctgctggaa atgataaacg
    36181 ccagtgaagc ccgcgaggac gacccggagt ttcaaagccc cgtcgatctc atgtttgagc
    36241 gtttggagca gaaagacccc gaccattttg cggtgcgcca gtacaagaaa ttcctgctgt
    36301 ccgcaggcaa gacccgttct tccatcctga tttcatgcgg tgccagactt gcgccctttg
    36361 acatccggga ggtacgcgag ctgatggagg acgacgaaat ggagctggac accatcgggg
    36421 acgaaaagac ggtgctgtat ctcattatga gcgatacgga cacgaccttt aatttcattc
    36481 tggctatgct ccaaagccag ctaatcaacc tgctttgtga ccgggctgat gatgtgtacg
    36541 gcgggcggct gcctgtccat gtacgcctga ttttggatga atttgccaac attggccaaa
    36601 tacccaattt cgataaactc attgccacca ttcgaagccg tgaaatatcg gcctctatta
    36661 ttttgcagag ccagtcgcag ctaaaagcga tttacaagga cgccgccgag atcatttcgg
    36721 ataactgcga tagtgtgctt ttccttagtg ggcgcggcaa aaatgccaag gagatttccg
    36781 atgcgctggg gaaagaaacg attgacagtt tcaacaccag cgagaaccgg ggaagccaaa
    36841 cgtcccacgg attgaactat caaaaattag gaaaggagtt gatgtcccaa gacgaaattg
    36901 ccgtgatgga cggcggcaag tgtatcttgc agctacgggg tgtgcgcccg tttttatcgg
    36961 agaaattcga tattaccaag catccccggt acaaatacct tgccgacgcc gacaagaaga
    37021 atacctttga cgtggacaga tttttgacta tcacgcgccg gaaacggcag caagtggtaa
    37081 cgcaggatga agtattcgac ctgtatgaaa tcgacctgtc ggacgaggac gccgccgctg
    37141 aataaacagc ggcttttttc attccaacaa caaaatcaaa aaatcattat gagccggaaa
    37201 ccggcagaaa gaggtaaatt tatggcattt tttaattccg cagttactac tttgcagact
    37261 atcgtgatcg gactgggcgg cgctctgtgc gtatggggcg gcatcaatct gttggaggga
    37321 tacgggcagg acaatccggg ggcaaaatcg cagggtgtca agcacttaat gtcttctatg
    37381 tgaaaagatt ttaaagagaa aatgcacatc ctacgcagat aacatgacaa tttcatgcaa
    37441 agcgaaagga gacaccatga aaatcgaata cacaaaagtg ggagactatt atttaccgaa
    37501 tctttactac ccggaggaaa ccagaccgat tggcttatgg ggaatgcttc ggaaggaata
    37561 tctgaaagaa cataaatctg gaacttatac atatcttctt ctgaccgcca ggctggacag
    37621 ttatttggca gatctgaatg aacaggcaca ggaacgcttt gaattgatcg aaactcagat
    37681 gcgaagtgcg gagggagttt ccgaggaact gaagagacag aatcctatgg aatggctatg
    37741 ccactgtaac aatatccgca accgggctgc tgagatcatc aagcaggaac tgatttatgt
    37801 ataaggggga ttctagatat gagaagagaa ctgattgaaa gttattatga agaataccag
    37861 cgggagctgg aaagatttct tgaatctgac ccggagatgt tcactctgga agcagaaatt
    37921 catcattaca tagaaatact ggaaccggtc ctcagagatg gcggacagga gatgtggcag
    37981 acactggaca ggattatttc agcgaaaaat gcaatggagg gaattgtggc aaaagaaatg
    38041 tatatccggg gatttctgga ttatgaaaga ctggtgtgtg aaaaagacag taagatctga
    38101 ataatcagaa catagactat atataacgag gcttttattg tatagacagt aagagcctca
    38161 tttaatatag ttttcataat aaaaaacagg tggtagtacg tatttgcttt gtgttatata
    38221 agaattggtc tggctgaaca atggtgaaaa tttactatga ttttacttaa atctgattga
    38281 aggatgaaaa agtgatgata tcatatagtg ggttgatagg gttacagaaa atgtattcac
    38341 agaggcagag aagaaataat gaaagtaaaa aatgcagatg aattttataa aatattagaa
    38401 agaaaggcta tatatccggt atttcagccg attgtaaatt tacagactgg agatgtggca
    38461 ggatatgaag ctcttagtcg tatcgacagg catgatacga ctcttatgat ttcagatttg
    38521 tttgtgattg ctgaacaggt aggctgtgtt tggaaattgg aaaaattgtg cagaaataaa
    38581 gcattgaagg cagcggcgaa taaaccagaa catgcaaaat tatttcttaa tgtagatggt
    38641 aatatcattc aggataaatc gttcatacag ggatttacaa accgtaaggc tgcgaaagcc
    38701 ggagtaccgt cctgtgacat tgtatttgaa ataacggaac ggtcggatat agaaaactat
    38761 cagattctgc aacagattat gaagcattat gcagatcagg gatatgaaat agctttggat
    38821 gatgttggag ctggatattc tggtctgaac cgtgtagtaa atacatcgcc aaactactta
    38881 aaagttgata tagaactggt cagagatatt caaaaacata agaaaaagga aattatgatg
    38941 gaatttctgt tgcattactg taatgagaca ggagcaattc tgattgcaga agggatagaa
    39001 acagaagcag agttggagtg tctgtgtaga ttaggtgttc attacggaca aggatatttt
    39061 cttggaagac cggacagaac gttcaaaaat gtaaggaatg aggcgatgtt ggtattacgt
    39121 agactgaata ataaatcgta atgaaaacta tatgtcagca ggtgaaaaat gaataagtca
    39181 attataacat tataaagatt tccattgatt tttacaccta ttttacacaa gcactcacat
    39241 agatttgatt tctgtcttct ataattgtat aggaaaatgt agatcttata taataacagg
    39301 agaggaaaga gaaatgaacg agaatgtgaa agtggttttt ggtctgatcg gtggccttgc
    39361 cttgttcctt tatggaatga acagcatgag tgatgcactt cagaaagccg caggagaacg
    39421 aatgaaaaaa atccttggat ttcttacaag aaatccaatt atgggagcac ttgccggagc
    39481 acttgtaaca gctgtattgc agagcagttc agcgacgaca gttatggtta tcggttttgt
    39541 cagtgcggga cttatgagtc ttccgcaggc aatttctgtt atctttggtg ccaacatagg
    39601 aaccactatg acggcacagc tgatggcatt taaaatcagc aattatattt atccaattat
    39661 ctttgttgga tttattctga actttgttag caaaaaagaa aaggttaaaa atatcggtat
    39721 ggtaattttt tcattcggac ttttatttga aggaattgaa atcatgggtg aagtcatgaa
    39781 acctttggcg ggaagtccgg tctttgttga tctgatggga aaagtttcat ccattccggt
    39841 tcttggtgtt gtactgggag cggtcatgac attggttgtg cagagtagtt cagctacgat
    39901 tgcagttctg cagaattttg catcacaggc aggaccggac ggagtaagca gtgtaatagg
    39961 gcttaccggt gcgattccga ttcttcttgg agataatata ggtacaacga tcacagcact
    40021 tcttgcatca attggacaat ctaaaaatgc aaaacgtaca gcaattgcgc atagtatttt
    40081 taatatcagt ggaagctgcg tttttatttt cctggttccg tggtttgcaa aatttgtcca
    40141 gtttatttca ccaaagggaa atgagattga tgtgatttca agacagattg caaatgcaca
    40201 tacaacgttt aatatcgtct gtacactggt ctggctgcca ctgattccgc tgatggtaaa
    40261 aattgttaca acaatcatcc gtggaaatga caaaacagag aaagcagctt ttgaacccaa
    40321 atatctggat atgaaagtga ttgaacagcc ggcggctgct atggttctgg tatctaagga
    40381 actgaaccga cttggagaac tggcagaatc tctgttaagt gatttaaaaa cggcgattgt
    40441 agcagatgga gactcaaaaa ctcatggttc gtttatagaa aatctggaga ttgtacatca
    40501 gttacaggac agtgtgacag aatatattac cagattattc gcaagcggaa accttacaga
    40561 acaacagtct gagcagacgg cagggttact gtatgtaaac aatagtattc agaggattgc
    40621 agacagatgt gaagatattg accagatatg tgaaaaagta aataacaatg gaaaaagact
    40681 gaccagtgaa gcctcctcag aaatggaaga ctgcattgat attatccagc agcttttaaa
    40741 gaaagcaatg gaggcaatcc gtaagggtga tgctgtagtg gctgaaacag tttttaaaaa
    40801 taagaaaaaa atgcataaag cagagaaaaa atacagtaag gcacatttaa accgtgtaaa
    40861 aaatgaggtt tgtgatgcgt ctatgacaag atatttttct gggatcatgt ataattttga
    40921 ccgtatggca gataattgtg tcagcattgc agaagaagcc tgtgataatg tggcttttat
    40981 aaaccttgat gaagagacgg gaaaatcaat gatggaaaga ggtgcagtat aaaatggtaa
    41041 agaaaaaaaa catccgaaaa tctgcacttc tgctgataat gataatgctg gcaacgggaa
    41101 taacagcatg tggaaccgag gaagagccat tgccggatct gtctgctatg ggagctgtca
    41161 cagctgtatc acgagaagaa gggtcaggaa ccagagcaga atttgagaat cttttgaaac
    41221 tgcctgagag tgatacagga atagttgttg attctacaga gaaagttctg aaaaaggtgg
    41281 aagaagataa aaacgcagtt ggttatgtcg catatagttc cgcaacagat acgaatggaa
    41341 aaatacttca aatcaacgga gttcttcctt ctgagaaaac aatagacaat aacagttatc
    41401 ctctttgcag ggattattat ctggcttata atggagaact gacagatgtg gaacaggatt
    41461 tcctgactta tgtaaagagt aaaggacagg atattgtgaa acaatattgt attcaggctg
    41521 acagtacaac aacattcctt tctgacaagt cagaaggaaa gattctgatt gaaggatcca
    41581 cttcaatgga accaatggta aaagcactgg cagatgacta tcagaaacaa aatccaaatg
    41641 ctgaaataga agtaaaagca acagattctt caagagggat tacggcggtg ataagtggag
    41701 agtgtgattt cgcaatgtca tccagagaat taaaggatta tgaagcagag cttctggaaa
    41761 caaaagtcat tggaaaagat gctatagcta ttgtgataaa tgaagaaaat ccactggaaa
    41821 atcttacaat aaaacaactt acaggtctgt acaatggaac atataaaaac tgggatgatc
    41881 tttcctgaga aaaacgaata taatatctta caagcactga ctataacggt cggtgcttct
    41941 tttttggatt ttttcaaaat tatctgacat tcttcaaatt cccacgtgtg attagtggaa
    42001 aaataatttt ttctgcaaaa gtaccgtcca aatgccctct gaaacttgct attagtgagg
    42061 gaatatttca tttatgcgaa aaaaacttgc aaaagggggt tggtaaatac cgcttcccag
    42121 gctttatagg tagaggaaca attattttaa aaattttacc ccaaagatgt ccgcttttgc
    42181 ctgtctcagt acagagtatg tgaagggact aacaaaagta aaagaaaaaa tgataaaaat
    42241 gcaaaaaaat atcacgaatc ttttgcatct tgcgaagtca tttgactcta gtaatcagag
    42301 gacgttttgg aatgttcctt cgatgaacct tgacaaccga taagtgcacc ctagaagcct
    42361 aacaggagtt atgccatgac ctttcttttt ctgagagcga gcgaaattat aatgacactc
    42421 ccgatgatgg gcggctgacc gaagccttat ggtcagtgac ctgctgatgc aggcagaggc
    42481 agtctgagac ggctgcaggt gacagggaaa cgcacgccag accgccggac gaatgaaact
    42541 gatgacaatg tttacagggc aaaatgcccg gaaaggagca gtttatgaca gaactgaaaa
    42601 tgaaagaaat gcagttgatt aactggtttg gatgcccgaa tctgatttat acaagggaga
    42661 gaatccatct ggttgccatg cttacaacag atgaagaaat gaaaaagaat ctttacaggg
    42721 tatgcagatt ccttgcaaga gaagaaatcg catatcggta tccgatcatg tatgaaatga
    42781 tccggaaaat attttcaccg gatgatacca atccaccgga agcatgtgca atgtttatgc
    42841 cggacgatga aacagatgat gaagaatatg caatggcagc gtagctgcgg aaaggacagt
    42901 aacgattatg aataaagtgg ttttaatggg aagactgaca aagaatccgg agatcaaata
    42961 tgccggaaaa gataatgata tggcagtagc aagatataca ctggcggtaa acagaagata
    43021 taaacgtgac ggtgaacagg aagcggattt tatttcctgt gtcacattcg gaaagagtgc
    43081 agagtttgca cagaaatatc ttcacaaggg aatgaggatt gtgattggcg gtcgtatcag
    43141 cactggcaat tataaggaca aggacgggaa aacaatttat actacagatg tgatcgtgga
    43201 agaacatgaa tttgcccaga acaaggataa tggtgttgga gctgattcat cggaaacacc
    43261 gaaaacagat aaggacggat ttatggaagc accggaaggt gaggttccat tcgactaaac
    43321 cgaagtaaat ggttggagag gttgcccttt gtcggggcat cctcttttcc attttacaga
    43381 agacatcaaa aattacaggt attcaaccta acgaaatcgt gatataatca aaatatgttc
    43441 agatgaaaaa tatggaggtt gcttatgggc gtaagttata acagattatg gaaaatgctc
    43501 atagataaaa atatgaagcg gatagagatg cagtacctca ccgggatcag tggaaatatt
    43561 cttgccagga tgggaaaaaa ccagtatgta tcaatggaga cgattgagaa gatctgcaag
    43621 aagctggact gcaccgttga tgagatgctg gaatttacag atgatgagta acaagagcag
    43681 acatgtcaaa ttattaaaac tggaagaatg cttcagaagt cgaaaaaaga aattgaaaaa
    43741 agataacacg ccggatacgg cataaatctc ttagcaacgc taagagcgca cttctataca
    43801 ggcaattcca tctgcctgta tcgtgcgacc tgcggtgctg ttcccgattt caagaaatct
    43861 caaaccggga aatacagcga aatcagagat ttcgtacaag gggaagatcc ccttgcggca
    43921 cagatgccta tccctgataa agtttgtgac tgaatatttc agatatagac atataagggg
    43981 aaagaaagtg tgaaatcaaa atttgtggag agattttatg attagaaaag caatactaaa
    44041 agatgttaat tcgattgaag atacttacaa tgagcatttc cagtatgaac tgaatcatac
    44101 agcctttact gtatttaaga aaggggttta tccaacgaaa gatgatgcag aacgggctat
    44161 ttatgctggt gctttgtttg tatatgaaga aaacggaaca attgttggaa gtatcattat
    44221 tgataaagtt caaccaatag aatatgcaac tattccttgg aaagaaaaat tatctgaaga
    44281 tgaggtaatg gtaattcacc ttttaatggt acgtccgagt atgtctggta aaggaattgc
    44341 atcatcacta ataaaattcg ctacagaatt agcacaaaaa aatagttgca gggcactacg
    44401 tttggataca ggaagtcaaa atattcctgc gctatctttg tatcaaaaaa atggatttga
    44461 aataattgct agtgcttcta aaaaagttgg agatgtaatt gcacacaaaa accatctttt
    44521 tctcgaaaag atattgtagt tcgtaaattc cagtttgaaa aattgagaaa atcggagttt
    44581 gaggagcatc accgttatga ttagagaatt acggaaagtg gatataaata aagttgcaga
    44641 aatatggtta gatacaaata taaagacaca ttattttatc tctgctcaat attggaaaag
    44701 taattttgag ttagtaaaag aacttctatt acaggcaact gtctatgttt acgaggataa
    44761 acaggaaata cagggtttta tagggttaag taacgaatac attgaaggta tttttgtttc
    44821 tgccgaaatg caatctcaag gtataggtaa aattttgcta aactatgtaa agggaaaaag
    44881 aaacaaatta atcttaaatg tatatcaaaa aaatacacgg gcaatatcct tttatcaaag
    44941 agaaggattt gagattcagt atagcggttt ggatgaagct actggagaaa aagactatgt
    45001 aatggcatgg cagcagaaat agaaattcaa gttttagtgt ttggaaaatt atgacttagt
    45061 gtgcaagttg ggagaaaata tggtaacgga aaaagagaaa aaagcatgtc gatttattgc
    45121 cgaatggcta taagattgtg agacagtctc tggtagatga tctggagggc gaaaaaaagg
    45181 tatgttggga gacagaaatt ttgcaggatg atgatagtgg atttacttat gaaatcagca
    45241 ggtgtctgta tttcgatacc tgtaaggaac atggctatgc agagttctgc aaagtatttt
    45301 gcaaccatga ctggtatgct tatggagtat taaagcatca ttcgagattt atcagaaaat
    45361 cgacgattgc cgaagatgga actgtatgtc atgatacaat cgagaaagta acagagaaac
    45421 attttttata aaaccgaaaa attgattatg ggaggaaatt gaaaatggcg aaagaagaga
    45481 gatttgaaaa aatatattct cagggagcat taactactac tgagatatgg gtggataagg
    45541 aaactggtgt gaattattta tatcatgcat gtgggtattc aggtggactg actccattgt
    45601 tggacagaga tgggaaacca gttatttctc aggctataaa tagatgacct tagaaacagc
    45661 aactgttatg gtaagtaaca gcatcgtcac aatatgtata tggtcgattg gtgtgtgcgg
    45721 taatatacta tattcagaaa aacaattcag gagattttta tgagaagatc aggttggaca
    45781 aaatggttga tcgtgattcc ggcaatgcta ggcattgtgt tggtgaccta tgtgcggtat
    45841 acaacggata tctggggatt tggtgcattt atctgccagc tgattgcaat tttggaattg
    45901 gcttatggtc tgcgaattgc gatgctggca cagaatagaa agaagtccta tcggttaaca
    45961 ccggaagaac gacatgagta tgcacggtat ttatatgaga agcaatatca ccgttaccct
    46021 gctgtagcga atcagatgct tttggttatg gcacggatga gtgtactcct taacaattat
    46081 gaacgggctg cacaggagct ggcagatatt cgtatagata aattcaatcc agcacagttg
    46141 aagttgtatt attacctgaa agtggttaca gcaatggcag caggagatgc gacagggatt
    46201 caagagagcc agatgtgtta cgcagggatt ccggatacta aatgcacata tccgtcaaaa
    46261 gcagagcttg ctatgtggat agagcagaga gcggcagcgc agatggcaga ggcactgaag
    46321 aaggcagtgc cggataaaaa agagcatccg atacggatca gtatcattac tttggttctt
    46381 gcgtatagta ccttcttcta tggattgtgg tatggtataa aacgtgatat gggatatgaa
    46441 gtgagatatc gtttcgcaga aatatcgatt actgttatta caatcagtct ggcggttctt
    46501 ttgatctggg ggatcgtgag tttatacaga cacaattgcc aggcattatc catacaattc
    46561 ggtaaaaaca aagtggcatt gataattgtc tatggagctg ctgcgatatt tgcacttttg
    46621 attattggaa tgagtggtct gagtgtagtg ttcggaatgg atggaacaga aacggtgact
    46681 ggttgtgaca gcaaatatac gtatataact gtgcaggaag attatggtgg tgaacggaga
    46741 tacagaacca ataatccatt tgttatgcag agtatggatg gtctgtggtg gtcttcggat
    46801 acaatagatg aagaagataa tcaagaagag gatactacag aattaccgga tttggataca
    46861 gagaattcaa cagattcaga ggatattcag agtggaaata ccatagaaga ttacgagaca
    46921 gaagggcagc atgatggtct gacaattcaa aatgcaatgc tggcagttta taattacctt
    46981 caggagcaga atacacttca aaatatggcg ttcagttaca cggcgaatgc aaagggagaa
    47041 gtctatgctg tcgtcagcga aacgcaggaa acaaagaacg gtaatacggt aaatgtgaga
    47101 tactgtctgt acgataatgg ttccaagaca gatgcagatg gtaattcctg cgaagaactg
    47161 gtgttagaaa aagtatatcc ggatggcaat tacgagaccg aattggtaga tttttatctg
    47221 gtaaatgcag atacgatgca ggtgacagac gaacagaaga atacgtggta aaataaattc
    47281 tggggaagta ggtgcattgt atgacaatga gagaaaaaat aaaactatta tcgtaaatgt
    47341 ttcaagcgga ctgggttctt ttggaagtat gcaaaaggac tccaagacat aagaggtgaa
    47401 ctgcgctgat ccaggtgcca cggcaacaga tttgaatgtc gacggaggaa agcaaacagt
    47461 acaggcgcaa tcgtaaaact tgcaatgatt gataaaaccg gactaacagt aacttttatt
    47521 agtcgatttg gaaatatgcc ttggtaatca acaattccgg ttttactgac taagaaaatg
    47581 aggaggattc ataatgagtg agaaaacaga gattactttt atgcaaacaa gactaatccg
    47641 gcttgcttca gaagagtggc atttacctgt tgaacagatt atccacttgt ttaaagaagc
    47701 cgatgttctt ggatatatag aaaaatgcta tgggattttt cattgtgaag gcgatgaagc
    47761 tgtgcttgaa gatattactg aatttttgca aagaaagggg atagagatta gtgcttgagt
    47821 taaaagatgg atttgttctc tatcatggaa gttattgtga agtaaaagag ccagatcttg
    47881 ccaaatgtgc aaagagaaaa gattttggac agggatttta tttaaccaca tcaaaggaac
    47941 aggcagaaag ttttctgaga acctcaatag ccaaagccat cgcaaccgga acaattgaag
    48001 aggggcagaa gtttggatat atttcaactt ttgaattcaa actttcagaa aatcttgaaa
    48061 cgcatatttt tgaaaatgca gatgtggact ggttacattg tatcgccgct caccgaaaga
    48121 aaaaaatgtt tattgaggtt gaacgtgaaa tggcaaaata tgatatcatt gcaggaaaga
    48181 ttgcggatga tgcaacaaac gctactctta ccgcttatct tgcgggtgct tttgggactg
    48241 caggtgataa agtagcagac gatttctgta tcagacagtt gctgccgaat aaattgaaag
    48301 atcaatactg ctttaaaact gaggcagcta ttgagtgtct gaaatttgtg aaaggtgaga
    48361 agatatggct gaagtaacaa ttccaaagga aaaaatgaat tatacaatcg accttctgat
    48421 cactatggtg acagatgaaa tcgcagaaga gacaggcaag gacagaaacg aggtattgac
    48481 agattttttg tgttcaaaga caggaaaagc actgtatgat gagaagacaa agctgtggtg
    48541 cactggtccg gcatatatag ctgaactgta tatggaagaa ttaaagaaat cctgatatgg
    48601 gatgtgagtt taaatttata cgacaatggt gacaaagact ttcattccaa ggagcaacaa
    48661 ttaaatattt caaaaacgct gaggtcagca tttcctaatc acaggacaac gacctcagtt
    48721 ttttataccc aggaatcggc attcatgaat aagccggttc tttttgttta cagaaaaaag
    48781 aaggagtaca agaaaatgag cagagcaaaa aatccaaagt atgaatccat gtcattggag
    48841 gaactgaagg ccaacatgga aaagagcaga caggaactgg aggcagcaat tcaccacaaa
    48901 aacattctgg agcagcggaa gaagctggta gaacgcaggg aacgttccca ccggctgatt
    48961 gtcaaagggg cagagtttga gaaagtattt ccgctgtcca gagatctgga acaggaagac
    49021 gtccaggaca tgatgaatca actgcagaac agcagttaca acaagagtat tgtgcggcag
    49081 gctcatatag ccgccttaca caaagatcaa cagcagattg cagaagcagt aaaaaagaca
    49141 gagaagggag atgacagtta atggcactgt atcatttttc agtaaggaat gtatccagag
    49201 ggaaagggca gatggtggtc gcttccgcag cctatatttc cgggcagagg atttttgaca
    49261 gttattacaa caagatacac gattatacga gcaagtcagg tgtgatattt acagaaatcc
    49321 tgacaccgga gtatgtgcca gagcgtctga ctgacaggga aacactctgg aacgaggtgg
    49381 agcatgtgga acggaacaac aaggcacagc ttgcctattc gtttgacatt gcactgcaga
    49441 atgaattgac acttgaggaa aatatcagac tggcaaaaga gttctgccag gagcagtttg
    49501 tggcacgtgg catgatcgtg gatctggcag tccatgaggg aaaagcggaa aatgaagaag
    49561 aaccggataa tccccatttc catgtgcttg tcccaatccg tccttttaca gaggaaggaa
    49621 tctggggcaa taagcagaga agggaatata ttcttgatga ggatggaaac cgggtgaagg
    49681 atgccaaagg aaaagatatg ttcaatgctg tttctactac cggatggaat gatccggagc
    49741 ttttaaaaga atggcggcgg gcatggacag agaaagtaaa tgggaaattc agggagtgcc
    49801 acatggcatc cagaatcgac caccgttcct acaaagaaca ggggattgac ctgatcccga
    49861 ccatccatga gggatatgaa gtccgggcaa tggagaaaaa gggaatcaaa acggtgatcg
    49921 gagaattgaa ccgggcaatc cggcagttta accagatgtt tatttctctg aaagaatcca
    49981 ttcagtggat gaagacagct tatgaggaaa tgaaagcaga gctggatcgc aggcagaacc
    50041 cgacactgct ggaaagcctg caggattatt atgataaaaa gacacagggc agaccaccac
    50101 ttcccaattt ttatgcggag atgaaacaga gaggtaagaa tctctccaat ttacaggagt
    50161 ttgcaaaatc catcagttat cttcagacac atcagataga aacgatggat gacttgcagg
    50221 aacggattga ggaactgaac ggtgttgttt ctgtcagtaa aaaagaaatt tctgaaaagc
    50281 ggaaacagtt aaaggaactt gagaatctgg aaaagatggc agaggtaata aaaacaaatc
    50341 agccattgat tgatgagtat aaccatttct attttcagaa aagacgggag agatattatc
    50401 agcagcataa aaaagaaatc aattattacc gcaaatgtga acgggaactg aaacaacatc
    50461 tggataaaaa tggaaaagtg ccaactgcca gatggaaacg ggagaaagaa gaacttcagg
    50521 cggggatcga agagctgaaa gcagataatc agccgtacca ggaggaacta acttttgtga
    50581 aaaaggtaca gagctgtgcg gatattgcca gacgtgaccg ggaaatggca gaagcagata
    50641 cttccgggcg ttcagaagaa aaaagggaga agcaggtgaa gttcccgaca ttttacacag
    50701 ttcaggcgaa agagattctt gaagaaaacg ggaaagcaga gcaacagccg ttgaaccagt
    50761 cggaacagaa tccagaaaag aagacctctc ttctgagaaa actggatgaa aaaaagaaag
    50821 agtgtgcaga gcgtgatgca aaacaacagg cagtgaagaa aaagcgaaac tatgacatga
    50881 gtttataaca gtctgtcagc aggacagacg ggaaaggatg agcaaaatgc agtatacaga
    50941 agaacaaatt ataagagcaa atcagacaga tcttgtttct tttctgagcg cacagggaga
    51001 acaactaggg aaaagcggaa aggaataccg ttggaaaaag catgacagtg taacggtatc
    51061 cggaaaccgg tggtacaggc acagtcaggg gaggggtggc tatccggtgg attttgtgat
    51121 ggaattttac aatgccacat ttccggaagc tgtcaagatg ctaaccgggg aggaaggcga
    51181 aggcaggaac agtacctgcc ccgccccgtc ccctgacttc cgattaccag agaaagagga
    51241 aaataatgat agaatcatca gatatctgac agaaaacaga gggattgaaa agaatatggt
    51301 ggaggaatgg atcggcagtg gggatattta tgaagaaaag aagcaccata atgtggtgtt
    51361 tgttggcaga gatgcagacg gtattccaag atatgcccat tgcaggggaa ctggtgaaac
    51421 aaaataccgg ggagatgtgg ctgaatctga taaatcttac ggattctgtc acagagggac
    51481 ggataatcag ttgtttgtgt ttgaagcagc cattgatctg ctttctttta tccaattatt
    51541 tcccaaagac tggaaaaagc ggagctacct gagtctgggt ggcatttcca gtgcggcact
    51601 gatggctttt ctttctgaac gccctcagat tacttcggtt ttcctgtgcc tggataatga
    51661 ccatgcaggg aatgaagcca gtgagaaatt ggcaatagaa atcccggacg gatacagtgt
    51721 catccgtctg aaaccgtccc gaaaggactg gaatgaaatc ctctgtgata aaaatgcaga
    51781 caggaagaaa tcaatcattg aaacagttac aatgaaagta ccggagaagg aagaactggt
    51841 gccgatgttg tgttatgaag acattgaaca gaccagtgta gaatggctgt ggtttcccta
    51901 tctgccattt ggaaaactga cgattattca gggcaatcca ggagagggaa aaacttattt
    51961 tgccatgatg ctgaccgcag cctgcacgaa ccgtaagact ttcccgaaca tggaagagat
    52021 tgaacctttt aatgtgatct atcagactgc agaagacggt atgggagata cgattaagcc
    52081 gaggctggtg gaagcaggag cagacctgag tcgggtgatg gtcattgatg atacagagga
    52141 agcattgacg ttatccgatg accgtattga aaaagcaatc cgtcagaatc aggtcagact
    52201 tttaatcatt gatccggtac aggcttttat cggtgccgat gtggatatga accgggcaaa
    52261 tgaagtccgt ccggtattcc ggaaactggg aatgatcgct gaaaagaccg gttgtgcgat
    52321 tgtgctgatc ggacatctga ataaatcttc agggacacag agtacttacc ggggacttgg
    52381 ttctattgat atcatggcgg cggtgagaag cctgcttttt atcgggaaag taaagaaaga
    52441 cccgacaaca agagtactga tccatgagaa aagttctctt gcacctccag gtgagacgat
    52501 ggcatttaaa cttggagatg aagaaggatt ccgctgggtg ggagcttatg agatttccgc
    52561 agatgatctt ctggacggaa aggaaggaaa gccgacagaa acaaaactgc aacggggaac
    52621 gaaactgatc tatgaactgc ttgcagacgg aaatgcagtc acaatccggg aactggatga
    52681 aaaggcaaaa gcacagggaa tctcccagag aaccatgcga gaagccagaa gccggatgaa
    52741 agaagaactg gattatcgga tgaatgaaaa gcaggaaaat accattcgat taaaaaaaca
    52801 agggagaatg ggtgacggca gaattttgga gtgaacggca ggctttatag ataccctatc
    52861 tgccgtctgc cgtctgccgg tgcaaggcaa tcaaacggaa atagcaggta tccatacaga
    52921 ataaggattg ctgtgaatct ggaacaaaaa tgaattgtaa tatggcggat ataatggtat
    52981 gattatccgg taacagcaat gttacgggga agcacctgtg acagggtgtg gctgcctcct
    53041 gatatttaca gaccggagcc tttcggggtt ctgagatgaa agaaaggagg tgcgtgatat
    53101 gacagcttat gagattatct caatattctt aggcgtactc agtttactga tcgcttttgg
    53161 tagcctgtta attgcgatta ttgcctttct tgatcggaag aacaagagga aataaaaatg
    53221 cctatcctgt ctgccaacag gataggcggt ggtcgtaaga ccatttagtc aactcaggcg
    53281 gcatccactt tggagtgggt gcttttccta taacaaatgt accacacatt cctcttgttt
    53341 tcaatgggaa aattaaaata tgcagttcat ctgacaggga tttatccctg tctttattat
    53401 atccccaatt tctgaaatgt taatacaaaa attttaatat atgtaaccaa cagtctgggc
    53461 agttaatctg taaaaatgga ttagctgctc tttttttgca aaaaatggag gttttacaat
    53521 ggcagtgaag aagaaaatta tcaacatcag agtatatttt gacggtgatc aggacgcaaa
    53581 agaagtgttt gcagaggtga tcgcacagaa aatacgtgac acgaaaacaa aagataaact
    53641 tgcaataatg aaagataagc agtataatga ttatgagttc agtaatctga aatctgcgta
    53701 ggatgtgcaa agtaacggag gtatcagatg aacaacagat atatgagtaa caatatagaa
    53761 agaaatgcaa cctggagagt tggtgtatac tgccgacttt ccaaagatga cgaattgacc
    53821 ggagagagtg ccagcatctc caaccagaga gacatactgg tgaattactg tcagtcacag
    53881 ggctggcagg tggtggatgt gtttcaggac gatggttata caggattgaa cacagaccga
    53941 cctgatttgc agagacttct caaagcctgt gaaaaaggac tggtgaatct tgtaattacc
    54001 aaagaccaga gccgactggg acgaaaccat gtacagacag gatttctgat ggaggagttt
    54061 ttcccaaagc atggagtaag gtatatcgca ttatatgata atgtggatac ctttgccgga
    54121 gacaatgaga ttgccccatt taagaacgtg ctgaacgaga tgtattccag agatatttcc
    54181 aagaaagtcc atgcgtccta tcacctgcag gcaaccaagg gaaagtttac aggtgttgtt
    54241 ccaccactgg gatatcagaa agacccggag gaaaagggac atctcctgat tgacgacgag
    54301 acagcaccga ttgtccggaa aattttccag tgggcggtgg acggtcatgg gattaacttt
    54361 atatccagag aactggaacg ccagcagatt ccctgcccgt catggtggca ccggaaacgg
    54421 ggactccgca cctattacac aaaatgggaa aagaaagacc cggaaaatgg gaaatatgta
    54481 tgggatgaat cctatctgaa atccctgctg atgaatcccg tttactgtgg tgacatggct
    54541 tcccagaaaa ggtactaccg tttcaagatt ggcaatcagg gagataaagc tccggaggac
    54601 tggatcacag tcagggatac ccacgaagca atcattccgc aggatgtatt tgatctggta
    54661 caggcaaaga tgaaaggcag gaaacgccag acagaacagg gagaatacag catgtttgcc
    54721 ggattactcc gctgtgcaga gtgtggcggt gcgttgacgt tgaagaagac gcacaccaaa
    54781 gaccagcatg aagtttatac ctgttccact tatatccata aggggaaagc acattgtacc
    54841 cagcaccggg tggacgctga tgacctgtat gatgcagtcc ttaccagaat ccaggaatgt
    54901 gcaaaagcag ttaccggaga cggaacagaa ctggaaaacc gggtaaagga gttatgcgag
    54961 gaagatacac agggacacag ggaaagtctg gagaagctgg tatcaaagca gaaagaccgc
    55021 ctggaaacgt tagaccggct gattgcaaaa ctgtatgatg accttatcaa tgacaagatt
    55081 acagaatctg tttttgataa gatgctggag aagacacaga aggagcagac agatatcaaa
    55141 aaggaacttt tccagaatga gagtgtgctg aatacggaag aaaaactgga tgcacagtcc
    55201 cagcagtgga ttgacgatat tagcgagtat gcggacatca aagaactgga tgccaatctt
    55261 ctgaaccggc tgatcagtaa aatcgttatc tcagaaccgc aggagaaaga cggaacagaa
    55321 aattactgcc gtactcctga aacggtcaca atggaaattc atttcaactt aaaaccaata
    55381 ccggagcttg gaaccatcga aagaggttct ggctcccata aataagaaaa atctatcaaa
    55441 atttaatcta ttttaatctt ttcaaggctc atttcgagca aatattttct cataaaagac
    55501 ataaaacagc ttgtcgctgg cggcggcgtg gccctgatcg gtgtcactct ggttcccctg
    55561 ctgtccgggc tgctgggctg atcctggcct ttgtccctgt ccaaaccctt aactaatcgc
    55621 ctccgcgtcc tcccggccag accgggaggg cgcgggaaag gaggtgctac ccgtgattag
    55681 tgaaatcatt gaagaatgga taaaaggcat cctcatggac ggtatcacgg gcaacctgtc
    55741 cgggctgttc gatacggtaa atgccaaagt cggagaaatc gccgccgacg tgggacgaac
    55801 cccgcaggca tggaacagcg gtattttcaa tatgctgcac agcctgtcgg aaaccgtgat
    55861 cgtccccata gccggggcca tactcgccct tgtcatgtgc tatgagctta tccagatgat
    55921 aacggaaaag aacaatatgc acgactttga cagctccatg ttcttccgct ggatattcaa
    55981 atccgcgttt gcaatcctga tcgtaacgaa cacatggaac atcgtcatgg gcgtattcga
    56041 cgccacccaa agcgtcgtca atcaaagcgc cggtgtgatt atcgggcaga catccattga
    56101 ttttaacacc ctgataccag accttgaaag ccaattggag gctatgagcg tgggcgctct
    56161 tttggggctg tggttccaaa cccttgttgt gggcctcacc atgaatatcc tttccatctg
    56221 cattttcctt gtgacctatg gccgcatggt ggaaatctat gttgtcacgt cgctggggcc
    56281 tatccccctt gccacaatgg gcaacgcgga atggcgcggc atgggccaag ggtatctgaa
    56341 atccctgctg gcgctgggct tccaagcctt tttaatcatg gttgtggtgg gtatctacgc
    56401 ggtgctgata cagaatatcg gttccgcttc cgacatatcc ggcgcaatct ggggctgtat
    56461 gggctatacg gtgctgctgt gcttctgcct gttcaagacc ggcagtatat caaaggccgt
    56521 attcggggcg cattgatccc aaaatccaag catacacagg aggaacttgt ttatttcaac
    56581 gtacaacatc atttacgctg acccgccgtg gcggtatgac tgcaaaaacg tacagggcgc
    56641 agcggaaaat cactattcca ccatgagcat tgacgagctg tgcgccctgc cggtggaacg
    56701 gctggcgtcc aaagactgcc tgctgttttt gtgggccaca ttccccatgc tgccggaagc
    56761 tctgcggtta atcaaagcgt ggggcttttc attcaagacc gtggcttttg tatggctgaa
    56821 acagaaccgt aaaagcctta catggtttta cgggctgggc cgctggacaa ggggaaacgc
    56881 ggaaatctgc ctgctggcaa cacacggcaa gccgaagcgt cgttctgcct ctgtccatca
    56941 gtttattatc agcccgattg aacaacacag caaaaagccg gatgtgaccc gtgaaaaaat
    57001 cgtagagctg gcgggcgatt tgccccgcgt ggagctgttt gccagacaga aaacccccgg
    57061 ctgggacgtg tgggggaacg aggtggaatg tgaccttatc ctgacgtgaa aggaggtgcc
    57121 aaatggctta tgtaaccgtc cccaaggact taacccatgt gaaaagcaag gttgtgtttg
    57181 ggcttaccaa acggcaactg atctgttttt ccggggcgct ggtgatcggc gtccccttat
    57241 attttctcac ccgtgactat cttaccaaca gcgcggcggc cctgctcatg gtatttgcca
    57301 tgctgccggg ctttttgctt gccatgtacg agcgtcacgg ccagcccctt gaagtggtga
    57361 ttatgcagat catccagtgc tgcttcatcc agccaaagga gcgtccctat cagaccgaca
    57421 acacctacac cgcccttgtg cggcaatacc agatggaaca ggaggtaaag gccattgtcc
    57481 aaaaaggaaa tcccaagaga aaccgaaaaa acaaagctga cccgcgccca gcgaaaagaa
    57541 attgacgcgg taatccgtaa atataagggc gacggcaggc cccacacggc gcagcagagc
    57601 atcccctacg aggtcatgta cccggacggc gtgtgccggg tatcgcccgg cgtcttttcc
    57661 aagtgcattg agtttgcgga catcagctat cagcttgccc agccggacac gcagacggct
    57721 atctttgaaa aactgtgcga cctgtataac tatgtggacg cctccattca tatccagttt
    57781 tctttcctca atcggaaagt tgacccggtg cagtacgcca agagctttga gatcgcgccg
    57841 cagggggacg acttcgacga catccgggcc gagtacaccg gcattctgca aaagcagctt
    57901 gcaaacggca acaacggcat ggtaaagacc aaatacctga cattcaccat cgaggccgaa
    57961 agcgtgaaag cggcgcgagc cagattgaag cgcatcggct ttgacctgtt aggctatttc
    58021 aagagcatgg gggccgtagc ccatgtgatg gacggctggg aacggctgaa cctgctgcat
    58081 ggcgtgtacc acccggacgg cgagatattc aattttgact ggaagtggct ggccccgtcc
    58141 ggcctctcca ccaaggattt tattgccccg tcctccctgt gcttcggcaa tgcaaagaca
    58201 ttcggcatgg gcggcaaata cggcgcggta agtttcctgc aaatcctgtc cccggagctg
    58261 tcggacgata tgctggccga cttcctcaat acggaaagcg gtgttttagt caatctccat
    58321 gtgcaggcca ttgaacagac caaggcgatc aagaccatca agcgcaaaat taccgacctt
    58381 gacgctatga agattgcgga gcagaaaaag gccgtccgca gcggttacga tatggacatt
    58441 cttcccagcg accttgccac ctacggcgag gacgccaaga agctgctgac aaagttgcag
    58501 acccgcaatg aacggctgtt ccagttgacg ttccttgtcc tcaacgtggc cgacaccaag
    58561 caaaagctga ataacgacgt gttccaagcc gcaggcgtgg cccaaaagca taactgtccc
    58621 cttgtccggc tggactacca gcaggaacag ggccttgcaa gcagcctgcc gctgggcgtc
    58681 aatcagatca agatacagcg cagccttacg acttccagcg tggccgtgtt cgtccccttt
    58741 gtgacgcagg agctttttca gggcggcgcg gccatgtatt acggcatcaa cgccaagtcc
    58801 cgcaacatga ttatgctgga caggaagcag gcccgttgcc ccaatgcctt gaagctcggc
    58861 acccccggca gcggcaaatc catgagctgt aaatccgaga tcgtcagtgt gttcttaacg
    58921 acgcctgacg atatttttat ttcagacccg gaggccgaat attaccccct tgtcaaacgg
    58981 ctccacggac aggtaatccg gctgtcgcct accagcaagg actttgtgaa cccgctggac
    59041 atcaacctga attattcgga ggacgataac ccgctggccc tgaaatccga ctttgtgctg
    59101 tcgttctgtg agcttgtcat gggcggcaag aacgggctgg aagccattga aaagaccgtg
    59161 atagaccggg ctgtgcgcgt gatctaccgg ccctatctgg cagacccccg cccggagaat
    59221 atgccgatcc tgtccgacct ccacaaagcg ttgttagatc agcatgtccc ggaggctgac
    59281 cgggtggcgc aggcccttga cctgtatgtg tccggctccc tgaacgtatt taaccaccgt
    59341 accaacgtgg acatcggcaa ccggcttgtg tcctttgaca ttaaggagct gggcaagcag
    59401 ctcaaaaagc tggggatgct cattgtccaa gaccagatat gggggcgcgt cacggccaac
    59461 cgaagccaag gaaaggccac atggtatttt gccgacgagt tccatttgct tttgaaagag
    59521 gaacagacgg cggcctattc tgcggagatt tggaagcggt ttaggaaatg gggcggcatt
    59581 ccaacaggtg ccacccagaa cgtcaaagac ctgctttcct ccccggaaat tgaaaatatc
    59641 cttgaaaaca gcgacttcat tacgctgctc aatcaggcgt ccggggatag aaaaatcctt
    59701 gcggaacgct tgaatttgtc caccgaacag cagaagtaca tcgacaattc cgaacccggc
    59761 gagggccttt tgatctttga aaacgtggtg ctgcccttta ccaaccccat cccgcacaac
    59821 acccagcttt acaagattat gacgacccgc ttaaacgagg tggcgggagt atgacccggc
    59881 gacgccacaa aggaggtgaa ccaccatgaa agagctgaaa gccaaggcga aagtgacgca
    59941 ggccatgacc cgcgacgggc tgacgctgga aaatcaggcc accggcgagg tggagaacat
    60001 ttccagccgg gaggccgaac aggatttgtc cgcgccgggg ccgggcggca ccgccgataa
    60061 gctgctggaa cgggccgacg ccatccatga cggccataag gccaagaaag ccgcgaagca
    60121 ggccggggct gtggccgccg agggggacgc tgccctgcac cggccatccg cccggctcca
    60181 actgtcggag gaagaacggg ccgacccgga tttacagccc tatatccgaa aatcggacaa
    60241 acgggccgat aagctggata aggcccgcgc cgccatcccc aagaagcgcg tcccggtcaa
    60301 ggaaaccgtc tttgacgagg caagcggcaa agcaaagacc gtcctgcact ttgacaaaca
    60361 ggacaagggg cctccctccc tgaaacccaa tccggcaggc cgtcctgttt ccgaggtttt
    60421 tctattcggc cacgggaaaa tccatgaagt cgagcatgaa aacgtgggtg tggagggcgg
    60481 ccataagggg gaggaacttg ccgagcggca ggccggaaaa gccatccgca gcaccatccg
    60541 gcaccacagg ctgaaaccct actgggacgc ggaaaaggcc gagcgcaggc tattgtctgc
    60601 caacgcggag tatttctacc agaaatcttt gcgggataac ccggagatcg cacaggcggc
    60661 cagcaacccc atttcccggt tctggcagaa acagcagatc aagcgccagt acgccaaggc
    60721 cgcccggaca gcgggccaga ccgcaggcgc ggcggcacag ggcgcggcca agacagccgg
    60781 ggcctccacg aaagcggcca aggccgcagc caaagaaagt gaacgggcgg cctcctttgt
    60841 ggcccgccac tggaaagggg cgctggccgt ccttgtgttg gggcttttga tgctgcttat
    60901 catcggcggc ttacagtcct gcaccgccat gttcggcagc gccggaagcg gcatcacggc
    60961 gtcctcttac ttttccgagg atagcgatat gttggccgcc gaggacgcct acgcggagct
    61021 ggaagccgag ctgcaagact atctggacaa ctacgaggcc acccatgact atgacgaata
    61081 ccattttgac cttgacgaga tcagccatga cccctatgtg ctgatttcca ttctgtcggc
    61141 gctccatgac gggatattta ccatcgggga ggtacagggt gatttgtcca tgctctttga
    61201 caagcaatat atcctgacag aaaacgtcgt ggtagaaacc cgttaccgca ccgaaaccga
    61261 tacatggacg gacgcggagg gcaacaccca tacagacacc taccaagtgc cgtatgacta
    61321 ttacatctgc tacgtcacgc tggaaaactt cgacctgtcc catgtgccag tctatatcat
    61381 ggacgaggaa acgctaagtt tctattccct gtatatgtcc acgctaggaa accggcctga
    61441 tctgttttcc ggcagccagt accccaacgc ctccaccctg acgccgccga cctactacga
    61501 cgtacccccg gaggctttgg aggatgccac ctttgccgcc atgctcaaag aggcggaaaa
    61561 atatatcggt tatccctatg tgtggggcgg cagctctccc agcaccagct ttgattgcag
    61621 cggcttcatt tcgtgggtaa tcaatcatag tggatggaac gtgggacggc tgggggcgca
    61681 gggcctatat aacatctgca cccctgtttc cagttcgcag gccaagcccg gcgaccttgt
    61741 atttttcaag ggaacctacg acacccccgg cgtgtcccac gtcggcctgt atgtgggcaa
    61801 caatgtgatg ctgcattgcg ggaacccgat ttcctacacg aaccttactt caaactactg
    61861 gcaatcccat ttttacagtt acgggcgttt accttaacgc cagaaaggag ctttcaatta
    61921 tggcaaccaa acttgaccgc atcgaaaagg acatccagaa aaccaagtct aagatcgcgg
    61981 agtttcagaa acagcttcgg gagctggaaa cgcagaaaac cgaacaggag aatttgcaga
    62041 tcatccagct tgtgcgcggc atgaacatga agccggagga atttgccgcg ttcttacgca
    62101 gcggcgcaat gcaggccgcc cctgccgcaa ccccttacca tgaacaggag gacaatgccc
    62161 atgaagaata agaccagacg gatttcccgt attctccccg cacttctggc cgtgatgctc
    62221 tgcgtcacgg cctttcccgt taccgccctt gcaggcggca atgacccggc accggccccg
    62281 ctgccggagg ctacaaccga acccgctacg ggcggtttgg agcctgaaac agacgaaacc
    62341 ggcaccgcca cggacgggac agaaaccgag gccgaaaaag ataccggccc tctgaccggc
    62401 aaggacatca ccgacctgtt ttccgcgctg ttcggttcca aggtgagcat tgccgccacg
    62461 gacgacggca tccagatcac caccggcacc gacaaaaaag aacctgaaca gaccggcacc
    62521 gtcaccacca acggcggcag gctgaatgtc cgcaccggcg cggggcttga caagaccgcg
    62581 ttcacccagc ttcccaatgg gacgacggtt gaggtagtcg gcacagacgg cgactggatt
    62641 aaaattctct tgccggagcg catcggctat gtccattccg actatatgac cgtcagcgaa
    62701 aaggaagtag cggctaccgg cgagggcggt ttttccctct ccattgaccc ggaggaaatt
    62761 gcttctctgc tggaactgtt caatggcggc ggggccagcg ccgccctgtc cccggacggg
    62821 aacctgtcac tgattgacga catcggcagc gccgcgaaaa atggcaagca gtttatcacc
    62881 gtggaaacca aaaacggcaa tgtgttttat ctgattattg accgggacga cgagggaaaa
    62941 gaaactgtcc atttcttaaa tcaggtggat gaagccgatc tgctgacgct gatggaggac
    63001 ggggaaaccg tcacccccgc tgctgtctgc tcctgtacca cgaaatgcaa ggccggggct
    63061 gtgaacacga attgcccggt atgtaaaacc aacctgaccg agtgcagcgg cccggagccg
    63121 caggaacccc agccggagga acccgaagcc ccggaggaag aacccaaggg cggcgcgggc
    63181 ggcctgatcg tgttcctgct ggtggcgctg gcgggcggcg gtgcggccct ttactatttc
    63241 aagttcaaaa agcccaaggc tgaaaccacc ggcagcgacg atttgggcga gtatgatttt
    63301 ggggaggacg aggatttgga ggacgaggaa cccgaaaccg agtttgaccc ggaagctgat
    63361 tttgtgagtg aacaggagga cgaggaatga catataaact tgtaatcgcg gaaaaaccca
    63421 gcgtggcccg cagcattgcg ggcgtgatcg gggccacgga gaaacacgac ggctatttgg
    63481 aggggaacgg ctatctggtg agctggtgca tcggacatct ggtgtccttt gccgacgccg
    63541 gacgctatga cgagcgtttc aaaaaatggc gctatgagga tttgcccatt ctcccggaaa
    63601 catggcagta catcgtcccc gacgacaaga aacagcagtt tgacgttctc tgttcgctga
    63661 tgcagcgccc cgacgtgacc ggccttgtgt gcgccaccga cgccgggcgc gagggggaat
    63721 tgattttccg tttcgtctac cagatggcgg gatgccaaaa gccctttgag cgcctttgga
    63781 tttcctctat ggaggacagt gctattaagg acggctttgc ccacctgaaa ccgggcggcg
    63841 actatgatcc gctgtatcaa tcggccctgt gccgggccaa ggccgactgg ctgatcggca
    63901 ttaacgccac ccggcttttt tccgtcctgt accacaagaa cctgactgtg ggccgcgtcc
    63961 agacgccgac cttaaaaatg ctggtggaca gggacgccaa gattaccgat ttcaagaaag
    64021 agaaatatca catcgtccat atcacggcgg gcggcgcgga cgcggtaagc agccgctttt
    64081 ccgacgctgc cgaggcaaac accgtcaagg cggcttgtgc aaaagcgcag gccgtttgcg
    64141 tttccgtgac gcgggagaaa aagacggaac agccgcccaa gctctatgac cttaccacct
    64201 tgcagcggga ggctaaccgg cttttcggct ataccgccaa gcagaccctt gactatgccc
    64261 aggcactgta tctaaagaag ctgctgacct atccgagaac ggatagtcaa tatcttaccg
    64321 acgatatgct gcccgccgtg gaaagccttg tgtccggcct gtgggagctg gtgccgtttg
    64381 ccaagggcct gaacctttcc ccgcagtttg accgcatcct gaacagcaaa aaggtgtccg
    64441 accaccacgc cattatcccc accgctgaat ttgtgaagca gggctttggc actctggccg
    64501 agagtgaaaa aaagctgctg tccctgatct gctgcaagct cttatgtgcc gtggccgggc
    64561 cgcatatcta tgaggccgtc accgccacat ttacctgtgc cgggcaggag tttaccgcca
    64621 agggcaagac cgtcctttcc cccggctgga aagagattga acgccggttc cgttcttcct
    64681 taaagaccga cgccgacgag gacgccgagg cggtgcggga gctgccggag ctttgcgagg
    64741 gccagacctt tgcagacgtg gccgcctctg tcaccgagca tttcaccacg ccgcccaagc
    64801 cctacaccga agatacgctt ttgtccgcta tggaacgggc cggggccgag gatatgccgg
    64861 aggatgccga gcgcaaggga ttaggcacac cggccacccg cgccgccatt ctggaaaagc
    64921 tggtgcagat gggctttgtc cagagaaaag gcaagcagct cattcccacc aaggacggca
    64981 tcaatctggc tgtggtgctg ccggaggcgc ttacttctcc ccagcttact gccgaatggg
    65041 aaaaccggct gaccgagatc gccaaggggc aggccgatcc tgacgaattt atggacggca
    65101 tcgagacgca ggcgcgggag ctggtgcaga cctattcctg tatcagtgag gaaaagcaga
    65161 aattgtttca ggccgagcgt gttcctatcg ggacgtgtcc ccgctgtggt gaggctgtct
    65221 atgagggcaa gaaaaactac tactgcggaa accgggcctg ccagttcgtc atgtggaaga
    65281 atgaccgatt ttttgaggaa cgcaagaaag cgttcacccc gaagatcgcc gctgcgctgc
    65341 tcaaaaccgg caaagtcaag gtaaagggcc tctattccgt aaagaccggc aaaacctacg
    65401 acgggacggt gcttttggct gataccgggg gcaagtacgt caactaccgc attgaacaca
    65461 ggagctaaag gacagtaaca cagcaagcat agggagtgtg gctacactcc cggtaaaatc
    65521 ccccggccgc gtccctgccg ggccggggat ttttgcttgt tggggaagtc cccaaccccc
    65581 tattttttga aacagaacag agaaaggagt gaccgcgtga acgaagtatt ttctatccaa
    65641 gtccataacc ggcagttttt ggaacagggc aaacaaggcg tctggctgga tttgcccacc
    65701 acaaccgaaa cgctgcaagc cgccctgcgg gatgttggca ttactgccga caatccgcag
    65761 gattttttta tcaacgggta ttcctgcccg gaggacagac accttgccat tccctatgac
    65821 atggtgctgg ccgccgacgt ggacgagctg aatttccttg ccgcccggct gggccagctt
    65881 gacgccgccg agatcgcaga gctgaacgcc gccctgcaaa atccccgtgg cggctttgag
    65941 aatatcggcc agatcatcga ctacaccgaa aatgtggatt actatgtgca tcttcccgac
    66001 gttagggggg atggacagtt gggcgactac tacctgaacc gttccggcat ggtggatatg
    66061 ccggaggaat ggaaagccgg gattgatacg gcgcagtttg gacgccacat tgcccagcag
    66121 gaacaaggcg tttttaccca gtacggctat ctggtgagaa gcggcgacga atggcagcgc
    66181 gtccatgagg gccagccggt gccggaggaa taccgggtga tgcactaccc ccagccggaa
    66241 gctgaccggg aggaaccgca ggcccagcag ccggaggcat ccgaaagccc cgacaaagcg
    66301 ccgcccccgg ttatcccgat catgctggac ggcatgaaca gcaaagaacg catgaaagtg
    66361 attactgacc gtctggaaac cggcgtacag gagctttttg aaagtagccg ctatatggct
    66421 tacttatccg ctatgtccaa gatgcacgat tacagtctga acaatacgct gctgatcgcc
    66481 atgcagggcg gttcgattgt ggcgggctat aacaggtggc aggatgaatt tgaccgccat
    66541 gtgaagcggg gcgagaaagg cattaagata ctggccccgg ccccgttcaa ggttaagcag
    66601 cagatggaga aaatcgaccc ggccacccag cagcccgtga tcggcgcgga cggcaagccc
    66661 gtgaccgaag aacaggaagt gacggttccg gcctttaagg tggtgacagt ctttgacatc
    66721 agccagaccg agggcaagga ttttcccatg ccctatatca gtgaacttac cggcgacgtg
    66781 cagcagtatc aggatttctt tgccgcgctg gaacggacat caccctatcc cataagcatt
    66841 gagccgattt cagacggggc caagggccgc tgcttctacg caaaacagcg cattgccatc
    66901 aacgagggca tgagcgaatt gcagacccta aagaccgcca tccatgagat tgcccatgcc
    66961 aagctccacg ccattgaccg ggaggccccc gccgaggaac aggcggcccg gccagaccgc
    67021 agcacccgcg aggtacaggc cgaaagcgtg gcctataccg tctgccagta ttacgggctg
    67081 gacacgtcgg actattcttt tggctatgtg gccgattgga gcagcggcag ggagttatcc
    67141 gagctgaaag cctcccttga aaccatccgg gccacggcca gcgaattgat aaccgagatc
    67201 gacggccatt ttgcccagct tcagcaggag cgcgaggccc ggcaggaaca ggaggccgtc
    67261 acggttgacg ttaccccgga ggcagcggcc ccggcgcagg ccgaggaaga acaggccccg
    67321 gcccttgatc cggcagccga gccgcttgtg actgtgctat ggagcgaaag cccccagcta
    67381 aaggacggcc agcaaatgcc gttgcatgag gccgacgcgc tgtttgcccg tctggatgca
    67441 gcatatccgg cagaggcagg ctatgacaaa accgcgttcc gcattgactg tatctttcag
    67501 ggcgagccgg acagctacga ggggcggcag gattttggag atagggacgg ttccctgatc
    67561 gaacatatcc agagctacca ccaattttac gcgcaggacg aaaactggaa aaagcatgta
    67621 atcgctacaa ccggcataga gggctgggag caggacaagg cccatcggga tatgctgtta
    67681 ggcgagtttg tcccctatat gaagctgcat tgcagccttg cccaaatgga acagacggcg
    67741 cagggccgct tacagtccgg ggaaaccctg acgccggagg aaacggccta tttgggggcg
    67801 cttctctctt atgtgcagga gtgccgcccc atgctcaatc agggccagta tcagttgccg
    67861 gagccgccga aactggctga cttcgaccag agcttgcagg actacaaagc acaggtacag
    67921 gccgagatcg cgcaggaggc cgccgcagcc ggtatgaccg tggaggaata cgcggcggct
    67981 ggctatgaag caccccaaca ggacaccttt tccatctacc agcttcgagg cggggaggaa
    68041 acccgcgact atcggtttga gccatacgag cgtttacagg ccgcagggct ggccgtggac
    68101 agtgccaact atgaccttgt ttatacggca ccgctggata aaagcacgtc tttggaggat
    68161 atttaccgca cattcaacat cgaccacccg gcagatttta ggggacacag cctttccgtt
    68221 tccgacgtgg tggtgctgca ccagaatgga caggacaccg cccactatgt agacagcttc
    68281 ggctatatgg aagtgccgga gtttttgcgg gaaaatcccc tgcgtaccgc cgaactgtct
    68341 acggagcaga acgaaaacat gattgacggc attatcaaca atacgccgcc cgaaccgcag
    68401 ccggaggaac ccgaacagct atcttttgag gatgtggcag ttgcttccgg ccagacacag
    68461 gaggaacccc cggaggctgc gcccccgcaa acggctgtcc gctattatcc catcaatgag
    68521 ggagccgccc gccgcgccaa ggaagccatg agcttttctg actaccggcc cggcagcgcc
    68581 acggcggaat accggcacta tgtcgatcag gccgtggaaa ttgcggagcg ccagaaaaag
    68641 cgtgtggagc cagagtatca cgaaaagata gacagcctcc ttgacaccta cgcccgcaag
    68701 ctggccgaga acatgaataa gggctatgag attaccgccc gcgtcccctc tgtcctgatt
    68761 gccgggccgt ccaatttccc ggtacgggcg aaagaaaagc agaacgccgc cagtgaccgc
    68821 aacatggagg aatttcagta cattcagggc ttgcttgaca aaatccgcag caccggcatg
    68881 ggtggtatca gcgccgacga tccgcaggcc gtttccaagc tggaaaagaa actggaaaag
    68941 ctggaagcgt cgcaggagtt gatgaaggcc gtcaatgcct actaccgcaa gcatgggacg
    69001 ctggacggat gcccccatct gaccgaaaga ggcattgaaa acctgaaagc ggatatggcg
    69061 tcgggctggc actatgagaa aaaaccgttc cagtcctggc agctctccaa caataacgcc
    69121 gagatacgcc ggttaaaggg ccgcattgag gaattgaccc gccaaaagga ggcggcctat
    69181 gtgggatggg agtttgacgg cggcactgtg gagatcaacc gggtggccaa ccgcctgcaa
    69241 atcttctttg aggggaagcc ggatgctgct gtacgcgacg agctgaaaag caacggtttc
    69301 cgctggtcgc ccaaggccgg ggcatggcag cggcagttaa acgacaccac aatccgcgtc
    69361 gctgaccgta tcaagtgcat ccagcccctt tccggcgaaa agcccagcgc cttacagctt
    69421 gcggcccgca gggagaaaga aaagccctct atccgggcgc agctcaacgc ggccaagacc
    69481 gaacaggcca agcagccacc ggcgcgggaa aaatcaaagg gattggaggt agagtaaatg
    69541 cagaagtttg aaaatgtgga cgttctggcc gcgctggaac agatcatgcg gcagaacacc
    69601 gcgttttatc aaagtgactt tggcattgac aaagacatta tcaggcggga tgccgccagc
    69661 gatcaggccg cagacaagac cctgctctgg atgtcccggc cctccgggac gtactgtttc
    69721 cgggaacgtg atgttttcct gaaagacacc cgacagtaca acacatggaa attttacggg
    69781 gagcagaccc gtgacaagat actggcctac gcggtggagc tgaccggcac acagggcggc
    69841 actatccggg gcaatctcta tgagctggac tatccgcagc atttccggca cgtcattgaa
    69901 accgcccagc cggtgagtgt gaaccggctc ttttatgagc atggcacccg tgacatcccg
    69961 gagagccagc gttttgacgg aagccctgac cgggtattgg gaaacttttt gtactatgag
    70021 gcccagcccc atgacccggc tgtgctgcag gaggcgctac ggacggagca gcacagccgg
    70081 gggcgggccg tccccggcga tttcaaagtc cacgtcaccg ctttgcatga cagccggatt
    70141 gaaaccgagg cccggcgtat tgtggacagc ctgaaagatc tgtccgcgcc taacagcccc
    70201 aataaaacgc atttcatggt ggagatctcc ccgcactttg cggcgattgc ttccaccaag
    70261 gataatgacc ggctctttgc catgctgccc tataaatccc tgtgttttac cgggatgaaa
    70321 ggcaggcatg gcgtgtatgc cgtgattaac cgggacgagc gccgggatgt gagtattcgc
    70381 aggccccggc cctctatccg caagcagcta tccgacagca aacaggccgc cagcccaaaa
    70441 aaggcggcgg ccagaaccaa aaagaatgaa ttggaggtat aaggaatgat gcactttacc
    70501 gtggaggaag aaaacctgat ctgtatgtac cacaatgcag accggcgcag aacgataaac
    70561 aggctcaccg ccgtcatgcc ggttatggat ggggatatgc gggcgcaggc ccagcagacc
    70621 ctttccaagc tggaacgcat gacggatgcc gactatgacg gccagaagtt ccacttcaca
    70681 gacgaataac gaacagcaga cgccgggcgc ggcggccatg tggtcgcttg cgtccggcgt
    70741 cttttctttt tgcctattcc tacgcattta gaacccggta ttgtcgggga taggatgaag
    70801 tattcgcttg cccctgcaaa catgcgggag aagccttgca gcgggcggtt ttcaatccct
    70861 taaatgcgta ggttgcagct ataattagac ccctgaaaca gatatagccg cgcagcgggt
    70921 gtatctgttt caggggtgtg caggtgatag ccgcaaagcg gcgtaagagg gtgcagccct
    70981 cttgccgtct atctctgttc cagcgggaag ctcggcaatg cttccagcag tttcagggaa
    71041 atgcgctgcc gtatatcttc gtcaatgact ttcttctttt tgccgtttgc ctgtctgatc
    71101 tccaccgtgg aaagctcgtt gatgtagtca gcataatggg ccaacacttt ttccgtggcc
    71161 cacctctcac cggcaacggc ggctttgatc gtttcatagt ccagcagctt ttcttgtccg
    71221 tccatcctga tacccccgtc agactgattt tttctatgat accatattcg gaagcatgga
    71281 accagagaaa acggcgcttt tctgctggta aattctacgc atttagaaca gcacttcggc
    71341 ggggatagga taaaacaccc acataccccg ctgaccatgc cggaaaagcc ttgaaatata
    71401 agggctttta cagctctaag tgcgtaggtt tccagtatct tttccgctgg cgctcggcct
    71461 ctttgcggcg gcgcatccgt ttggagcagg ccgcgcagta tttggcccgg ttagacttcg
    71521 gcgtgaacgt ggccccgcac tcacagcagc gtttcacttc gtcccggtct ttggtgatct
    71581 cggcatacag ggccgcatcc agcgggagga cagcggcctt aaaccagcgg cacagaagcg
    71641 aataggaaat gctctgtacg caaatacatt cttcgccgtc gtccagcaac aggcagttgc
    71701 cgtcacaata gttgcagcac tcatgcgtca gcttccgggc cttgcgatat tgagggtaat
    71761 tcattcgtgg aatactcata gctcttgcgt ccattccttt cccggcgtca tacgcaaaat
    71821 gccgtccaca ttctgcttga tgatgccgta ttcctgcatc tgctttttcg cttcgccgta
    71881 cttctcatac agccgttcct tttcctcggc caatcgcttg tactcggctt tcagggcgta
    71941 cagatcgggg agctttttca caccctgtcc ctttagggcc tttgccgcag cttcatacaa
    72001 aatcaacgtg ctttcatgct cacgccggaa ttttgcctta tccgctgcat tgcggtattc
    72061 catagccacg ggccagagct gcttgtaggt agaaatattc tttatcagca ccgccatatc
    72121 cgcaagccgg tgttccatag ctttgagggt agcggcggcc tcgtcgctgg cggcggtgat
    72181 ctcggccact ttgctttcca aatccgggta ggtgtctatc ccatgttcag tcaggaaatt
    72241 caaagtcttt gcggcctgct tcaaattgtg gagcttcgcc cacctctcat agcctgccga
    72301 ctgctgggcc ttaatgttgt tttccagatc aatccgcaaa gaaatcccct tgcgctcttt
    72361 gggcgctctg gtgataacgc ggcctttgat acgctctttg atggcttcct cggtgtaggc
    72421 ttcccccaat gtcttgcagc gggtaaagcg ttcctgtccg ggagcgcgga aagaaataaa
    72481 ctttcccggc ttgatctcat agccctgcgc ctgcatgagc cgcaggaaat catcaaaatc
    72541 ttttgcctgc gggatagcgg cgtcaatggc tgctttgagc ttcgctttcc agctcgtccc
    72601 tttccgctgg gcgtcccact cggcatagct cttgccctta ctctggccgg gcttcaccac
    72661 ggacaatccg ttttccttgc acagcctgtc gctggtgcgc cggatatagg catagctctg
    72721 tttgttggaa atgtacttgc gctgatttac catatccacg gcacagaaaa taatgtgatt
    72781 gtggacgtgg cccttatcaa tatgggtagt cagcacatag ggatatttcc cttgcagcac
    72841 ttcctcggca agctgcttcc cgatctcatg ggcctgttcc ggcgtggctt cgccgggggc
    72901 aaaggattgt atcaggtggt ggcccagatt attgcccttt tggtatgcct gcgccagcag
    72961 catttcaaat tcaatgtccg ctgtttcata agagcaggca aaggaggcca ccagcagctt
    73021 gccgtcagtt ttgtccgggt tctcgatgta gtcaagggct tttttcagcg ttgacttgat
    73081 aggatgtatc tttgtaactg ccatatctgc gccagcgccc cctttatatc ctcgatgtcc
    73141 tgtgcgtaca ccggccccat accgttcagc ctctttgcaa tctgattgac gttgacgccg
    73201 atcttctgta tctcggcggt ctgcgcccgg atgtcgctgg tgtccatgtt gacgatatag
    73261 ccgtcaatga gcatcttccg ggcgtaggct gaaaagttct tcgtatgcag cagcgccatt
    73321 ttctgttcaa tcatagcccg ttcctccggc gtcacaggga cgcgcagcac gatttttcgc
    73381 tttctgtttg ccatgcttca ccgtcctttc cattcagggg tttggggatt tccccaacaa
    73441 gcaaaacgga cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact
    73501 ccctatgctt gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta
    73561 cccgcaaaat agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag
    73621 ctcccgctaa aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg
    73681 gcttttcaat tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg
    73741 ggcagagttt cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct
    73801 ccgggttaaa gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg
    73861 gcagactggg aatggccccg tcgttttcga tgttggcaag atagcgccag tcaatattta
    73921 ttttctctgc cattgctttg cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg
    73981 cgccgaaagt ttcaaagccg ggacaatctt caatattcgc catatcgcac ctcccactta
    74041 cattgtatat ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta
    74101 tatttcacat tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag
    74161 gtgtactatg gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg
    74221 atggataaac tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat
    74281 ctggctcatt cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc
    74341 ggttactgaa tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa
    74401 gcatggtatt ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga
    74461 taaaaagtat tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc
    74521 gaaactttat catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg
    74581 tatcagccgt atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct
    74641 ccagacattg gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat
    74701 accggggagg tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac
    74761 atttcctgta actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt
    74821 ttctactgtc ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac
    74881 cggagggagt acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca
    74941 aaaaagaggc cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg
    75001 ccgcaggcgg gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg
    75061 ccagtattat cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag
    75121 gtgttaatta ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc
    75181 atgaaaagaa gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga
    75241 aactacaggg aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc
    75301 gatacgaatt ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct
    75361 ggctgaggca ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt
    75421 ttacgaaaac acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca
    75481 tgaacttttg cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct
    75541 gtcggatgac gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa
    75601 gccattcaag atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc
    75661 gctggcaaag gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact
    75721 ttgtgacggt acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt
    75781 gcagaacaaa ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga
    75841 ctttgagggt gatggcagca cagtccacac tcatatcaaa aatttgcggg ccaagctgcc
    75901 ggaaaatatc atcaaaacca ttcgcggtgt aggataccga ttggaggaat cataataatg
    75961 gaaagaaaag ggattttcat taaggttttt tcctatacga tcattgtcct gctactggtt
    76021 gtcggtgtaa cagcaacact gtttgcacag caatttgtgt cttatttcag agtgatggaa
    76081 ttacagcaaa cagtaaaatc ctatcagcca ttggtggaac tgattcagaa tagcgatagg
    76141 cttgatattc aagaggtggc agggctgttt cactacaata accaatcctt tgagttttat
    76201 attgaagata aagagggaag cgtactctat gccacaccaa atgccaatac atcaaatagt
    76261 tttagacccg actttcttta tgtggtacat agagatgata atatttcgat tgttgctcaa
    76321 aacaaggcag gtgtgggatt gctttatcaa gggctgacaa ttcggggaat tgttatgatt
    76381 gcgataatgg ttgtattcag ccttttatgc gcgtatatct ttgcgcggca aatgacaacg
    76441 ccgatcaaag ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg
    76501 ccgctggagc gaaaggatga gcttggcgca ctggctcatg atatgcattc catgtatgtc
    76561 aggctgaaag aaaccatcgc aaggctggag gatgaaatcg caagggaaca tgagttggag
    76621 gaaacacagc gatatttctt tgcggcagct tctcatgagc taaaaacgcc catcgcggct
    76681 acaagcgttc tgttggaggg aatgcttgga aatatcggtg actacaaaga tcattctaag
    76741 tatctgcgcg aatgcatcaa aatgatggat aggcagggca aaatcatttc cgaaatactg
    76801 gagcttgtca gcctgaatga tgggagaatc gtacccatag ctgaaccgtt ggacataggg
    76861 cgcacggttg ccgagttgct gcccgatttt caaaccttgg cagaggcaaa caaccagcgg
    76921 ttcgtcacag atattccagc cgggcaaatt gtcctgtccg atccgaggct gctccaaaag
    76981 gcactatcca atgtcatatt gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg
    77041 atatggagtg agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac
    77101 attgatgata ctgcgcttcc aaggctgttc cccccattct atcgcattga tcaggcgcga
    77161 agcagaaaaa gtgggcgaag cggtttagga cttgccatcg tacaaaaaac gctggatgcc
    77221 atgagcctcc agtatgcgct ggaaaacacc tcggatggcg ttttgttctg gctggattta
    77281 ccgctcacat caacattgta aatatttaaa acttaaatga ttttgaccga caggtataac
    77341 cctgccggtc tttttttgct gctacaggaa aactacagac tggctacagg gaaagtacag
    77401 atgcgcttgc cataataaca atcgtaccag ccacaaatcg tagttttatt gcaaaggagg
    77461 cattcgatca aatggaaaaa agcaactatc attccaatgc ggatcatcac aaacggcata
    77521 tgaaacaatc cgtggaaaag cgggcttttc tatgtgcgtt cattatttcg ttcacagtct
    77581 gcacgctgtt tttgaggtgg agactggctt ccgtattgga ggcaacacag ataccgccca
    77641 tccctgcaac tcatacaggc agcagcactg acgtagtgga gaatttggag gaaaacgctc
    77701 ttgccaccgc caaagaacag ggagatgaac aggaatggag cctgatttta gtgaacaggc
    77761 agaaccccat ccccgcacag tacgatgtgg agcttgagca actatcaaat ggtgagcgga
    77821 tagatattcg gatttctccc tatcttcaag atttgtttga tgccgcaaga actgatggag
    77881 tttacccgat tgtcgcatcc ggataccgaa caacagaaaa acagcaagaa attatggatg
    77941 aaaaaattgc cgaatataag gcgaaaggct acacctctgc acaggctaaa gcggaagcag
    78001 aaacttgggt ggccgtgccg ggaacgagcg agcatcagct tggtcttgct gtggatatca
    78061 atgcggacgg aattcattca acaggcaacg aggtttatag atggctggat gaaaacagct
    78121 atcgttttgg ttttattcgt cgctacccac cagacaagac agagataacc ggtgtgagca
    78181 acgagccgtg gcattaccga tatgttggca tcgaagctgc cacagagatg tacaaccaag
    78241 gggtttgcct tgaggaatat ttaaaaccag aaaaatgaga aaaggatgtt atgttatgga
    78301 cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca atgagacggg cgcagagaaa
    78361 aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc tatgcgcaga cgatagggga
    78421 aaaatcgctt tcccatttgc tctttgaaac ggattgcgca ttatacaacc acaataccgg
    78481 atttgacatg atataccagg aaaacaaggt gttcaactta aagctggcag caaagacctt
    78541 aaacggctta ttgataagac cgggggaaac cttttctttc tggtggctgg tacgccatgc
    78601 ggacaaagat accccctata aagacggcct tacggtgacc aatggtaaac tcaccaccat
    78661 gtcgggcggc ggtatgtgcc agatgagcaa tttactattt tggatgttcc tgcatacgcc
    78721 attgacaatt atccagcgca gaggtcacga agtaaaggag tttccagagc caaacagtga
    78781 cgagattaaa ggggtggatg caaccattcc agagggctgg attgatttaa aagtgcgaaa
    78841 cgataccgac tgcacctacc aaatatgggt gaccttagat gatgagaaaa tcatcggtca
    78901 ggtgtccgcc gacaaagagc cccaagcatt atacaaaatt acaaatggca gtatccagta
    78961 tgtccgtgaa agtggcggga tttatgaata tgcccaggtt aaacggatgc aagttgcctt
    79021 aggtaccggg gaaataatag attgcaagct gctttataca aacaaatgca aaatctgtta
    79081 tcccctcccg gaaagtgtgg atattcagga ggagaaccaa tgagaaaaag tgtgggcatt
    79141 actgtttttg gatgcgaaca ggatgaggca aatgctttcc gcgctttatc gccggatttt
    79201 catattatcc ctacgctgat tagcgatgcg atatcggcag acaacgcaaa attggccgct
    79261 ggcaatcaat gcgttagcgt aggccataag tccgaggttt ccgaggcgac aattcttgcg
    79321 ctgagaaagg tcggggtaaa atacatttct acccgcagca tcggctgcga tcacattgat
    79381 acgactgccg ccgagagaat gggaatctcg gttggcacgg ttgcgtattc gccggacagc
    79441 gttgcggatt atgctttgat gctgatgctg atggccatac ggggtgcaaa acccaccatg
    79501 cacgccgtgg cgcaacaaga tttcagattg gatcgtatcc gggggaaaga actgggggat
    79561 atgactgtgg gagttattgg aaccggccat atcgggcaag cggtcgtcaa aaggctgcgg
    79621 ggatttggat gccatgtgct ggcctatgat aacagccgaa aaatggatgc agattatgtc
    79681 cagcttgatg agcttctaaa aaacagcgat attgttacgc tccatgtgcc gctttgtgcg
    79741 gatacccgcc atctgatcgg tcagaagcaa attggagaga tgaagcaagg cgcattttta
    79801 atcaacaccg cgcgcggagc gcttgtcgat accggggcgc tggtggaggc gttggaaagc
    79861 ggaaagctgg gtggtgcggc actggatgtg ttggagggcg aggatcagtt tgtttatacc
    79921 gattgctcgc agaaagtgct tgaccatccc tttttgtcgc agcttttaag gatgccgaat
    79981 gtgatcatca caccccatac ggcgtactac actgagcgtg tgctgcagga tactacagaa
    80041 aaaacaatca ggaattgtct caattttgaa aggagtttac agcatgaata gaataaaagt
    80101 cgcaatcatc ttcggcggtt gctcggagga acatgatgtg tcggtaaaat ccgcaataga
    80161 aattgctgcg aacattgata cggaaaaatt cgatccgcac tacatcggaa ttacaaaaaa
    80221 cggtgtatgg aagctatgca agaagccatg tacggaatgg gaagccgaca gtctccccgc
    80281 catactctcc ccggatagga aaacgcatgg gctgcttgtc atgaaagaaa gcgaatacga
    80341 aacacggcgt attgatgtgg ctttcccggt tttgcatggc aaattcgggg aggatggtgc
    80401 gatacagggg ctgtttgtat tgtctggtat cccctatgtg ggctgtgata ttcaaagctc
    80461 cgcagcttgc atggacaaat cactggccta cattcttaca aaaaatgcgg gcatcgccgt
    80521 tcccgaattt caaattattg ataaaggtga caagccggag gcgggtgcgc ttacctaccc
    80581 tgtctttgtg aagccggcac ggtcaggttc gtcctttggc gtaaccaaag taaacggtac
    80641 ggaagaactt aacgctgcga tagaagcggc aggacaatat gatggaaaaa tcttaattga
    80701 gcaagcgatt tcgggctgtg aggtcgggtg tgcggtcatg gggaacgagg atgatttgat
    80761 tgtcggcgaa gtggatcaaa tccggctgag ccacggtatc ttccgcatcc atcaggaaaa
    80821 cgagccggaa aaaggctcag aaaatgcgat gattacagtt cccgcagaca ttccggtcga
    80881 ggaacgaaat cgggtgcagg aaacggcaaa gaaagtatat cgggtgcttg gatgcagagg
    80941 gcttgcccgt gttgatcttt ttttgcagga ggatggcggc atcgttctaa atgaggtcaa
    81001 taccatgccc ggttttacat cgtacagccg ctacccacgt atggtggccg ccgcaggaat
    81061 cacgcttcct gcactgattg acagcctgat tacattggcg ttaaagaggt gataagtatg
    81121 gaaaatggtt ttttgttttt agatgaaatg ttgcatggcg tccgttggga tgccaagtac
    81181 gccacatggg ataacttcac aggaaaaccg gtggatgggt atgaggtgaa tcgcatcatc
    81241 ggcacaaagg ccgtggcgtt tgctctgcgc gaggcacaaa tccatgcggc tgcgcttggc
    81301 tatggcttgc ttttatggga tggatatcgg ccaagaactg cggtggactg cttcctgcgt
    81361 tgggcagcgc aaccggagga caatctcaca aaagaaaaat tttaccccaa tatagagcga
    81421 gccgagttga ttacaaaggg ttatgtggcc tcacaatcca gccatagccg tggaagcgca
    81481 attgatctta cgctctacca cctggataca ggggaacttg tttcaatggg aagtaacttc
    81541 gattttatgg acgaacggtc gcatcataca gcaaaaggga taggggatgc agaggcacaa
    81601 aatcgaagat gcttgcgtaa aatcatggaa agcagcggat ttcagtctta tcgctttgaa
    81661 tggtggcact ataagttgat tgatgagcca taccccgata cctattttaa ttttgccgtt
    81721 tcataatgaa agtattggtt ttgtatcaga ttttggattt gataaaatct atgctggtca
    81781 tggacaatct tttactggaa agtggttaaa gcatcttttg taatacattg caaattatag
    81841 tattcaatct gcccagcggc agaaaagaaa aaaaccgctg ttgggcagac ctttcccacg
    81901 cccattttgt attttgctgg cggcggtttt gagaaatcaa ggccgccgtt cttttctggc
    81961 agcaaaaggt gtggcggccc ataggaggat gccgctatgc tgttttgcta ttgccatgct
    82021 ctaatccact tccaccactc aaaaaagcct tgcccctc
//
LOCUS       DACEDM010000003        77822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEDM010000003 DACEDM010000000
VERSION     DACEDM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.003"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            471..2108
                     /locus_tag="IWO50_000177"
     CDS             471..2108
                     /locus_tag="IWO50_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0357333.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IWO50_000178"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IWO50_000178"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0357334.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="IWO50_000179"
     CDS             complement(3184..3819)
                     /locus_tag="IWO50_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0357335.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IWO50_000180"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IWO50_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0357336.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6403
                     /locus_tag="IWO50_000181"
     CDS             5405..6403
                     /locus_tag="IWO50_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357337.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6416..8434
                     /locus_tag="IWO50_000182"
     CDS             6416..8434
                     /locus_tag="IWO50_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ0357338.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8494..9210)
                     /locus_tag="IWO50_000183"
     CDS             complement(8494..9210)
                     /locus_tag="IWO50_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0357339.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9437..9979)
                     /locus_tag="IWO50_000184"
     CDS             complement(9437..9979)
                     /locus_tag="IWO50_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0357340.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9984..10211)
                     /locus_tag="IWO50_000185"
     CDS             complement(9984..10211)
                     /locus_tag="IWO50_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ0357341.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10290..12170)
                     /locus_tag="IWO50_000186"
     CDS             complement(10290..12170)
                     /locus_tag="IWO50_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0357342.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12359..12973)
                     /locus_tag="IWO50_000187"
     CDS             complement(12359..12973)
                     /locus_tag="IWO50_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0357343.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            13115..13675
                     /locus_tag="IWO50_000188"
     CDS             13115..13675
                     /locus_tag="IWO50_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0357344.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13980..14294)
                     /locus_tag="IWO50_000189"
                     /pseudo
     CDS             complement(13980..14294)
                     /locus_tag="IWO50_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14548..16183)
                     /locus_tag="IWO50_000190"
                     /pseudo
     CDS             complement(14548..16183)
                     /locus_tag="IWO50_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16506..16985)
                     /gene="rlmH"
                     /locus_tag="IWO50_000191"
     CDS             complement(16506..16985)
                     /gene="rlmH"
                     /locus_tag="IWO50_000191"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0357345.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            17413..18702
                     /locus_tag="IWO50_000192"
     CDS             17413..18702
                     /locus_tag="IWO50_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0357346.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(18741..19346)
                     /locus_tag="IWO50_000193"
     CDS             complement(18741..19346)
                     /locus_tag="IWO50_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0357347.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(19551..20015)
                     /locus_tag="IWO50_000194"
     CDS             complement(19551..20015)
                     /locus_tag="IWO50_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0357348.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(20037..20357)
                     /locus_tag="IWO50_000195"
     CDS             complement(20037..20357)
                     /locus_tag="IWO50_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0357349.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(20501..21580)
                     /gene="pepA"
                     /locus_tag="IWO50_000196"
     CDS             complement(20501..21580)
                     /gene="pepA"
                     /locus_tag="IWO50_000196"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0357350.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            21780..22130
                     /locus_tag="IWO50_000197"
     CDS             21780..22130
                     /locus_tag="IWO50_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0357351.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            22353..23300
                     /locus_tag="IWO50_000198"
     CDS             22353..23300
                     /locus_tag="IWO50_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0357352.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(23357..24775)
                     /gene="pepV"
                     /locus_tag="IWO50_000199"
     CDS             complement(23357..24775)
                     /gene="pepV"
                     /locus_tag="IWO50_000199"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0357353.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            24924..25769
                     /locus_tag="IWO50_000200"
     CDS             24924..25769
                     /locus_tag="IWO50_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0357354.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            25773..26213
                     /locus_tag="IWO50_000201"
     CDS             25773..26213
                     /locus_tag="IWO50_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0357355.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            26263..26457
                     /locus_tag="IWO50_000202"
     CDS             26263..26457
                     /locus_tag="IWO50_000202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357356.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            26593..27279
                     /locus_tag="IWO50_000203"
     CDS             26593..27279
                     /locus_tag="IWO50_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0357357.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(27687..28376)
                     /locus_tag="IWO50_000204"
     CDS             complement(27687..28376)
                     /locus_tag="IWO50_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357358.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(28351..30480)
                     /locus_tag="IWO50_000205"
     CDS             complement(28351..30480)
                     /locus_tag="IWO50_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0357359.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(30528..31865)
                     /locus_tag="IWO50_000206"
     CDS             complement(30528..31865)
                     /locus_tag="IWO50_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0357360.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(32117..32476)
                     /gene="rplT"
                     /locus_tag="IWO50_000207"
     CDS             complement(32117..32476)
                     /gene="rplT"
                     /locus_tag="IWO50_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0357361.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(32529..32729)
                     /gene="rpmI"
                     /locus_tag="IWO50_000208"
     CDS             complement(32529..32729)
                     /gene="rpmI"
                     /locus_tag="IWO50_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0357362.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(32801..33325)
                     /gene="infC"
                     /locus_tag="IWO50_000209"
     CDS             complement(32801..33325)
                     /gene="infC"
                     /locus_tag="IWO50_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0357363.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33645..34277
                     /locus_tag="IWO50_000210"
     CDS             33645..34277
                     /locus_tag="IWO50_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0357364.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(34279..35184)
                     /locus_tag="IWO50_000211"
     CDS             complement(34279..35184)
                     /locus_tag="IWO50_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0357365.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(35269..35922)
                     /locus_tag="IWO50_000212"
     CDS             complement(35269..35922)
                     /locus_tag="IWO50_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0357366.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            36244..37188
                     /gene="mvk"
                     /locus_tag="IWO50_000213"
     CDS             36244..37188
                     /gene="mvk"
                     /locus_tag="IWO50_000213"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0357367.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            37181..38158
                     /gene="mvaD"
                     /locus_tag="IWO50_000214"
     CDS             37181..38158
                     /gene="mvaD"
                     /locus_tag="IWO50_000214"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0357368.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            38171..39256
                     /locus_tag="IWO50_000215"
     CDS             38171..39256
                     /locus_tag="IWO50_000215"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0357369.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            39253..40317
                     /locus_tag="IWO50_000216"
     CDS             39253..40317
                     /locus_tag="IWO50_000216"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0357370.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(40566..41369)
                     /locus_tag="IWO50_000217"
     CDS             complement(40566..41369)
                     /locus_tag="IWO50_000217"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0357371.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(41389..42231)
                     /gene="kduI"
                     /locus_tag="IWO50_000218"
     CDS             complement(41389..42231)
                     /gene="kduI"
                     /locus_tag="IWO50_000218"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ0357372.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(42247..43254)
                     /locus_tag="IWO50_000219"
     CDS             complement(42247..43254)
                     /locus_tag="IWO50_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ0357373.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(43261..43905)
                     /locus_tag="IWO50_000220"
     CDS             complement(43261..43905)
                     /locus_tag="IWO50_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0357374.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(44005..44763)
                     /locus_tag="IWO50_000221"
     CDS             complement(44005..44763)
                     /locus_tag="IWO50_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ0357375.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(44890..46041)
                     /gene="fucO"
                     /locus_tag="IWO50_000222"
     CDS             complement(44890..46041)
                     /gene="fucO"
                     /locus_tag="IWO50_000222"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ0357376.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(46123..46434)
                     /gene="rhaM"
                     /locus_tag="IWO50_000223"
     CDS             complement(46123..46434)
                     /gene="rhaM"
                     /locus_tag="IWO50_000223"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ0357377.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(46436..47281)
                     /gene="rhaD"
                     /locus_tag="IWO50_000224"
     CDS             complement(46436..47281)
                     /gene="rhaD"
                     /locus_tag="IWO50_000224"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ0357378.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(47295..48551)
                     /gene="rhaA"
                     /locus_tag="IWO50_000225"
     CDS             complement(47295..48551)
                     /gene="rhaA"
                     /locus_tag="IWO50_000225"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ0357379.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(48548..50008)
                     /gene="rhaB"
                     /locus_tag="IWO50_000226"
     CDS             complement(48548..50008)
                     /gene="rhaB"
                     /locus_tag="IWO50_000226"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ0357380.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            50161..51030
                     /locus_tag="IWO50_000227"
     CDS             50161..51030
                     /locus_tag="IWO50_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0357381.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(51156..52055)
                     /locus_tag="IWO50_000228"
     CDS             complement(51156..52055)
                     /locus_tag="IWO50_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0357382.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(52220..53044)
                     /locus_tag="IWO50_000229"
     CDS             complement(52220..53044)
                     /locus_tag="IWO50_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0357383.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(53055..54350)
                     /locus_tag="IWO50_000230"
     CDS             complement(53055..54350)
                     /locus_tag="IWO50_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ0357384.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(54366..54845)
                     /locus_tag="IWO50_000231"
     CDS             complement(54366..54845)
                     /locus_tag="IWO50_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0357385.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(54851..55879)
                     /locus_tag="IWO50_000232"
     CDS             complement(54851..55879)
                     /locus_tag="IWO50_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ0357386.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(55896..57002)
                     /locus_tag="IWO50_000233"
     CDS             complement(55896..57002)
                     /locus_tag="IWO50_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0357387.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(57231..59828)
                     /gene="adhE"
                     /locus_tag="IWO50_000234"
                     /gene_synonym="adhC"
     CDS             complement(57231..59828)
                     /gene="adhE"
                     /locus_tag="IWO50_000234"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0357388.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(60133..60513)
                     /gene="yajC"
                     /locus_tag="IWO50_000235"
     CDS             complement(60133..60513)
                     /gene="yajC"
                     /locus_tag="IWO50_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0357389.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(60610..61755)
                     /gene="tgt"
                     /locus_tag="IWO50_000236"
     CDS             complement(60610..61755)
                     /gene="tgt"
                     /locus_tag="IWO50_000236"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0357390.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            61973..62671
                     /locus_tag="IWO50_000237"
     CDS             61973..62671
                     /locus_tag="IWO50_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0357391.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(62839..65478)
                     /locus_tag="IWO50_000238"
     CDS             complement(62839..65478)
                     /locus_tag="IWO50_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0357392.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(66097..67128)
                     /gene="queA"
                     /locus_tag="IWO50_000239"
     CDS             complement(66097..67128)
                     /gene="queA"
                     /locus_tag="IWO50_000239"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0357393.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            67321..68496
                     /locus_tag="IWO50_000240"
     CDS             67321..68496
                     /locus_tag="IWO50_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0357394.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            68500..69141
                     /locus_tag="IWO50_000241"
     CDS             68500..69141
                     /locus_tag="IWO50_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0357395.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            69138..70055
                     /locus_tag="IWO50_000242"
     CDS             69138..70055
                     /locus_tag="IWO50_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0357396.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            70052..70723
                     /locus_tag="IWO50_000243"
     CDS             70052..70723
                     /locus_tag="IWO50_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0357397.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(71264..71752)
                     /locus_tag="IWO50_000244"
     CDS             complement(71264..71752)
                     /locus_tag="IWO50_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0357398.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(71953..72219)
                     /locus_tag="IWO50_000245"
     CDS             complement(71953..72219)
                     /locus_tag="IWO50_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0357399.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(72219..72482)
                     /locus_tag="IWO50_000246"
     CDS             complement(72219..72482)
                     /locus_tag="IWO50_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0357400.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(72475..72747)
                     /locus_tag="IWO50_000247"
     CDS             complement(72475..72747)
                     /locus_tag="IWO50_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357401.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            72958..73143
                     /locus_tag="IWO50_000248"
     CDS             72958..73143
                     /locus_tag="IWO50_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0357402.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(73285..75108)
                     /locus_tag="IWO50_000249"
     CDS             complement(73285..75108)
                     /locus_tag="IWO50_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0357403.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(75706..77670)
                     /locus_tag="IWO50_000250"
     CDS             complement(75706..77670)
                     /locus_tag="IWO50_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0357404.1"
                     /translation="MGEKVVALNDKMPELKDQAKIILDLQEKIPEIQNAGKQLAEIDG
                     DFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDLANQTKSGASQLKDAI
                     PSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKKNISNVLNGFIDGISQ
                     QQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQNRLSALNTAVQNEDTK
                     AIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTIGTAQGALSKADQIDF
                     DSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNMDTIVNGINEGADLYN
                     NELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVETAVNAAADLINNDWP
                     NIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQPVELQTNALYPIANNG
                     SASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQFVARMLTFLTMAIAQS
                     LIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALFDNVGKGIAIIILVLS
                     ISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWPNATKAIWIMIGLFVV
                     FGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT        22839 a        16237 c        13864 g        24882 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctaaggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacatat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttg cttctctaag acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactaa caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataaacga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc gattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgatatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatgc tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacggaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaataaaaga ctaacaaata aactgagcaa ggggttgtga
     8461 taaaagacat gtcacaaccc cttgtttatc tcattaattt gcttgtccat aaacgatttg
     8521 attaaagcca tttttcaatt cattattcgc tgtctcgttg aagtaaagat aattatcggg
     8581 attcccctga ttttgttgtg tcaccaatac ttttggctga ttttgctgaa acccgaatag
     8641 atagacgtgc tttttcagat atggttctgt ctctgcatcg gaatagacgg ctgtgatttg
     8701 atagtctgct tgacctgtcc cactttctga ccattctgca gaaacaggca ctccaccaat
     8761 agccatcttc cactccccgt tcaaaatagc ttgagggact ttaagaccat ataagtccac
     8821 actcatttga ttcgtataac ttttgtattg ttgtcccatt gtttggcccc atttggtcat
     8881 aaatgcctct aattgtgcgg atttgtctgc attccacagt gtttgttcaa cttgttcgct
     8941 gctttctgaa gaggaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt
     9001 ttcagtcgtt ttggaagact ctgacgaagt agaggtatct gctgtttctg tacttgctgc
     9061 agttgacttg ctctctgtgc tagttgttgt agtagttgca actgttgctt tcgctgtttc
     9121 ttttgttgat tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag
     9181 accaattgtt actaaaatcc tttttttcaa ttaaaaaact cctttcgatt actcccactt
     9241 ctttattcta tcaaataagc aatgtgattt tattgatttt tcgtattttc tcatcaatct
     9301 ttcaaatctt ctaaaaactg agaagcaccc gctgatgttg tctgtaatac atcagcgggt
     9361 gcttctttta ttttaatctt cgtgattttc caatttacct cacgctttat atttgtattt
     9421 gaagaaacat agacttttag tcatcatcgt cttcctcatc caaatcttgt cggacagttt
     9481 ttccttggag tctttgtact tggtagatga tttctttatt gaaacttaaa agttcaacaa
     9541 gcaattgttg caagccatat ttttcttctt tttcagctaa tttgattgcc cgtgtgataa
     9601 agacattttg gaaatcaaaa tcttttgcaa agttttccaa cattgttgag gctggtaaat
     9661 atttgttttg accatgttcg taaatttttc cataacgcat gacttcttct aaagtagacg
     9721 gaacgatttc attttcaatc actaactcat gagagatttt attgaaccat ctcgcttctt
     9781 gatcagcata ttctgaaaaa agggtactta attcttgcga agatgtaccc aaaacataca
     9841 attgtgcttg gatcaatttc atttcttcta atttaaaatt tgctaataca tgccccacca
     9901 tggcgccagc agttggttta tgatggtcga tatctgcttg ttttgtttct tgaacaaatt
     9961 tttcttccac tatattcatt gatttacgcc tcttttactt ttgctgattg gacttcataa
    10021 cctaattttt ccacaactgc agctaattgt tcagggtgga cttcatctgg atcaaagttt
    10081 gctttgattt tgctcgcatt aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa
    10141 accgcatgtt cgattttttg catacagctt ggacaagcta gtgtatctaa ttgcataata
    10201 atttttttca tcgtagttct tccttccttt ttcttgatag tcttattcta gattacagct
    10261 tccataaaaa aattgattct tatcaagttt tacgattttt cttctttgct ttcgtgtcca
    10321 aaatacaata aacgcatcgc gttaaagata acgatcaaga tactagcttc gtgaacgaac
    10381 atcccactag ccatgtaaat aaacccggca aataagccgt tgagtaaaaa taagacgaca
    10441 ccaatggcaa tcactacatt ttcacgggta tttaaaacag ttttcttagc caagcggtag
    10501 gcatgaacca acgattcaaa attagattgc atcaagacaa cgtcactcgt ttcgatagca
    10561 acatccgttc ctgatcccat agcaatcccg atatcagccg cagccaaaga gggagaatca
    10621 ttgattccat ctccaataaa agcaacgcgc aatccttgtt cttggtattt tttcacaaat
    10681 tgaacctttt cgtctggtaa taattcagca tggacttcat caatgcctaa catatcagct
    10741 acataatcag cagtcatctg attatcacct gtcagcatca ctaactgttt tgctcctgct
    10801 tgtttcaact tagctaattg ttcagcgact tctggtcgaa tcacatctga cacacctatg
    10861 atttgagtga tttttttgtc gattgctaca ataatgaccg agctgcctgt ttgttgtaat
    10921 tgatgtaaat cttgtacttg ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg
    10981 gtgttgcccg cacaaactgt atgaccatca atctcagctg tgattccttg tcccttgatt
    11041 gtctgattat ctttgactgc taattgttga aaatcaatgt ctttctcctg agcataatgg
    11101 atcacggcgc gccccagagg atgatcggat aacgtttcta atcgagccac taaagctaag
    11161 ctgtcccctt catcctctgt atagttcttg atagtggata cagcagtagt tcctttggtc
    11221 aatgtaccag ttttatcaaa gacaaaagta tcgattttgg aaaaagtatc cattgcttca
    11281 ccgcctttga ctaagatgcc ccgtttcgct ccattaccga ttcctgctac attcgaaaca
    11341 ggcgcaccaa tgactaaagc gccaggacaa ccaagaacca aaacagtgat tgctaaacga
    11401 aaatcacgac taatcagacc aacaaccaac gcaataagta aaacggttgg agtataatac
    11461 tgagaaaatc gatcaatgaa tttttccgca tgggatttgg tatcttgtgc ttcttctact
    11521 aattcgataa ttttggcaaa ggtcgtatca tctccgacct tagttgcttc aatcgtcacg
    11581 tagccatctg ctaaaatcgt tccagagaag acttcatctc cactgatttt gacgacttgt
    11641 cgtgattcac cagtcactgc ggcttcatcc gcataccctt tcccgtcaat gactcgtccg
    11701 tccacaggaa tagctccacc agtttttact aagacgatat cgccttcttc cacgtcatcg
    11761 atttccactt cttcagtcgt tccatcttct tggacaacac tagcagtaga tggggccatc
    11821 tcggtcaaag ccttgattga ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta
    11881 ccaaacaaaa acaagaatgt aacgatagct gactccttgt attctccgat cataaaggca
    11941 ccaagcacag caatagtaac gagtaaatca attgagataa ctttgacttt cactgcctga
    12001 tacgcatgga tcataatagg tatgaccgca ataatagaag ctatggctag agcaatgtta
    12061 tagaacaaga caaaaccagt aaattttccg ataaaaccaa aaataatcaa cacagctgat
    12121 aaggcggtga tatgatttcg atgctcttgg atccactttt gtaatttcat ctttctccct
    12181 cttttccttt cattcttacg agactaagta tagatactta tccatcataa aaaattgacc
    12241 actatcaagt ttagtcaaaa aggcggtttt cttttggtag aatacactac aaaaaatagt
    12301 tagcaagaag actcactaaa agtgatcttg ccaactattg tcgtttctat cgaaattttc
    12361 attgaatgat tttcctgtac agcatacatc ggagatctat ttctttatct aagattttta
    12421 tcttgctaat tttttccttt agtctttcaa atccacgtcg ttcatagaat tgatacgtac
    12481 agccactatc tgtaaaaagg aatatttctt ttcctttctc tcttttttca aattctgtta
    12541 gcaacttggt accgatgcct tttgttttca aatcaggatt tgcagctaaa aaaatgatct
    12601 ctccatctgg cgaatgcttc tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt
    12661 aagctccttt actttcttta gcaaatatat tttgtaaaaa atcgaatatc ttaacatata
    12721 tttgctgtcc aaaagaacga tgtttctttt tttctccttt tatctccgcc agcaaaacac
    12781 ctgccaactc gtcctctgca tacaaagcaa gaatttgagt tgctcgattc aactccatat
    12841 acaaaaaata tcttccgtaa agattcagca gaagttcatt ttccatatat ctctcaaagt
    12901 tcattccttc aacagcaaaa tagatagctt ttttgtaatc tttcttttct aagggtttaa
    12961 aagttagttt catcatttcg cctcctcaac ttcaatgatt gtgttatact tacattataa
    13021 tagacacagt gtctactatc tataatcaat catctataag tttgtcgatt aagcgacagc
    13081 tttgctttga tatgtcggat aaggagatag gtaaatgaaa aagacagata gacgcgtcaa
    13141 aaaaacagaa aaagctttgg ctgaaacact atccacatta ttagtaaata aaaagattca
    13201 agcaatcact atacgcgaac taacagaaac ggcagatgta catcgttcga ctttttatac
    13261 acattataag gatatctatg atctatatga tcaattagaa agcaactttt ttcgggatct
    13321 aaatgaaatt ctctcttatg atccagccca tagctatgag gaactataca ctcgcttgat
    13381 tgactatgtt tacgcaaata cttttttata tcgcttattt atcctcaacc atggtgatat
    13441 ttcgtttcag aagaaagtta aacaagttat tgaagaaaac tatctgaaca tatggctata
    13501 tgaagacaag aaaacgatta tcacagaaga aatgagattc tttactacgt accacgtcca
    13561 aggttgtcta tccgttctca atcggtgggt agaaagcaat tacaagtact ccaaaaaaga
    13621 attattagaa ctcttacaga aactgaatga tcacatagag aaaatcatgc cttgaataaa
    13681 aacatactaa gcttagttaa gaagaaccgt aaccttctcg tataacaact gaatcttatt
    13741 tagccaaatt gtttactgaa gattttttga gtaaaatttc tataattcct acttattgaa
    13801 aagtagtttg tagtgctgct tctccctctc taagtgatat atcttgttgt accaatcaaa
    13861 ttcacctcat cattaataaa ttacttttag gctaatttat tacaacttat aggttgaagc
    13921 tgtaaccaat ttatagtcat attatatttt tatcatccag tttattgaat tcttctcgtt
    13981 tacctcaatt cattttcaag gggaatcagc tcatcatcta acattttacg ccatgctcgc
    14041 ttcatttctt gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg
    14101 taatccttac tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat
    14161 tgcagattag aagcatgtaa ttttttctca gaaacaaacc actcattgga aaagacatga
    14221 atcaaatcat cacttacttg tgtaaaatag cgacgtttaa tgacaaagat atcttcattg
    14281 ctatctagtg taccttcatt tctttgtgct taaattcaaa gataattttc tcaataaaga
    14341 tttttgattc cagtaagatt cctttactcc ttttacaaca tagcgttgtc ctttgttttg
    14401 aaacatcttc ttcccctctt tgccattaaa aatagcccaa ttctctataa agaagattaa
    14461 gcttcattat tataatataa agtttaaatt tggtttatta cttacttttt cttattctct
    14521 agtaagctaa tattgaaaaa aatgtaatca gatcattatt tttatatgtt gtaacttcct
    14581 gataagaaaa ctaattaaaa gaaagaggta actacataaa ctaattaaaa aagctaactt
    14641 catagttgga ggataaatgg aagaaacttc aatgattgtt tctccttgtg ttccttcaat
    14701 cgacactagt cctattatca atctctcttt tttgtccatt tacttttgcg actgttcccc
    14761 aataatcaaa tacaggaagc gttatttttt gtcctttaga ttcatgtact gtatagacta
    14821 ttttcgaatc catcacttga taaggcatag tatgccattc cttgccaatt cttacttcat
    14881 gattctgcaa atgttctttg tattgtaatg cttccgtggg tgcatagtca taaagtcctc
    14941 cgggaaggag tgtcgtgtaa cgaataagat tttcttgatt cattaccaag tgatctctct
    15001 gagtgacaac tttatgttca tagtttgctt ttgttgcagc agatacagcc aacagtaata
    15061 aaacaattgc cgttatatta actacttttc tttgcttagc tgatgtgctc ttcatgaaaa
    15121 cgaacgataa aatcaacgag ccaaacagta cttgaaagcc taagattcgc caaggaaatt
    15181 gaatcatttg aatgggtgta ttttgtaata aagaccatgg aaaaaatttg gtggataaga
    15241 gccaagagac taccgcacta aggtaaatca ctttttcttt gcgattaaat cctttaaaaa
    15301 agaagagtgg acagatcaaa gaaataatac caatgattcc taccgtaaag gttgtcaggt
    15361 cattattcat cgcaccaatc aaactatccg agacatttaa tgcttggtgt tgtaaggtcg
    15421 ttttatctgg ctcattaatc gattgaacca tgatttgtcg gaacatcgga taccaaaagt
    15481 atgctgttaa aagaatcgtc attcctactg cctttgacaa ctccacaatc aaccggatgc
    15541 tttctttttt tcttgtttca cttactacaa agccgcctaa gactagtaag ccaacggcta
    15601 ttacactcat aaaagtggaa agaacatgtg tataaatgag taagctcata ctgactgcta
    15661 atggaattgc acatggcttt ttctcataaa tcagtgcgta agcataatac ataactatcg
    15721 gtataaccgt aagtgcaatc gcttcagcaa tagcagaacg atgataaata tcaatcgtac
    15781 ggtacccata aaatgtatat aaacagctaa atataaaagc cgcatcgatt ctttttaaaa
    15841 attttagtcc ataatgaaac atcagcatga tagtacaaac attcaatagc caaacatata
    15901 agatataact aacgattaaa ttattactta tccaataaaa aatgactgcc ggaaaaaagg
    15961 tcaagtaggg ataaaaataa ttcactccgc tcccataatg attaaacgtc gtaaaattga
    16021 ttggaccaga aaaaattgaa gataaccctt tgactctagc aaaatggaag gcaatatccc
    16081 ccgatgtaga aatagtatga acatttctaa aataattgat tgaataaagt ccagcaataa
    16141 ttgctaggag aatggacaat attatttttt tctttacttg cattacttaa ttatatactc
    16201 ctttcatgga ttgctttatc tagtataact taaattttaa ataaatcgtt atttattttt
    16261 actctcactt aaaaaagcac taaaattata agagagggag gtaatggtct gaacaccaag
    16321 aattgttcca acatcgaatt gcttaactaa tgagttcaaa gcttaattat gtggctctta
    16381 tatttaccga gcgtggattg cgatctcttt agcgaaacaa acatctttca taaaaaaaac
    16441 agccatatcc gctttcttgg atatgactat ttactactac tttactatct ttcattcttc
    16501 ttgtctcact tatgataagg cgcccccgca ttgatccgga aggcacggta gatttgctcg
    16561 acaaggatca gacgcatcaa ttgatgtgga tacgtcattt ttccaaaaga gatctgagca
    16621 ttgcttcttt tcatgacggc ttcgctgagt cctaaagagc cgccgataac aaaaacaagg
    16681 tggctttttc cttggatacc taattggtcg atttgtttag caaaagattc acttgtagga
    16741 tttttccctt caattgctaa ggcatagacg tattcttgtt ctttgatttt cgctaagatc
    16801 cgttcccctt ctttttcttt tacttgtctc atttgcgctt cgcttaaatt ctccggggct
    16861 ttttcgtcag gaacttcgat caattcgatt tttgcatagg cattcagccg tttgacatac
    16921 tcattgatac cttggatcag gtatttttct tttaattttc ctactgagat aattttgata
    16981 ttcatgtttt ctctcctttg gtcttcatgc tcactttgtt tattataatc aattttttcg
    17041 tctatatgtt tattttggct atttttgtta aaaagatttc cccaacagtt atgcacaaca
    17101 tccacaggtt tatccacaga aaacagtgtt attttacctt atttcatcac attcattagg
    17161 caaacatacg ttttataaga atttatccac tttatacaca ggctgtggat atgttttatc
    17221 ccttattttt ccaatcctat ccgcgtagtt ttacacagta tctgtggaaa aagaaaatca
    17281 aatattttca tctgaaaaca atgatttttc ctaaagtttt tcataaaaag ttcacagtct
    17341 caaagtagac taaagtcaac aaataagact atactaacag tagataaatg aatgtaattg
    17401 gaggaggaac acatggatag aaaaaatgtg acaccaaaga tgaaaaaaag taaaaataat
    17461 agcctctggc gtaagttagg tctggggctt gttggaggga tcgttggagg actactgaca
    17521 gctggcattt tttatgcagc gatgggcact ggcaacaata gttctactgc tacaagcggc
    17581 aatcagaatt ctgccggtga aacagttgtg gaaaatgtca aagtcaatgt agattctgat
    17641 atcaccaaag cggtagataa agttcaagac gctgtcgttt ctgttatcaa cttacaaagt
    17701 caaaaccaat caagcggttt tggcggacta ttcgggcagc aagaagaaag tagcagctca
    17761 gatgatagta atttggaagc ttatagtgaa ggaagcggcg tgatctacaa aaaagacggg
    17821 aatactgctt atgtagtcac gaataatcac gtagtagatg gccagcaagg tcttgaagtc
    17881 ttgatgaaag acggtacgaa ggtaaaagct gaattagttg gaacagatgc ttattcagat
    17941 ttagcggtgt tgaagatcaa ttcagacaaa gtcgaaacag ttgcctcttt tggtgactca
    18001 agtgctctga aggttggtga gcctgcgatt gcgattggtt ccccactagg atctgaatat
    18061 gcaaattctg tcacttcagg aatcatttct tccttgaacc gacaagtgac aagtacgaat
    18121 gaatcgaacg aaacagtcaa tatcaatgcg atccaaacag atgcagcaat caaccctgga
    18181 aattctggtg gtcctttagt taatatcgaa ggacaagtta tcggtatcaa ctcaagtaag
    18241 atcgcaagta cttctgaatc tacttctaat gtaagcgtag aaggaatggg atttgctatt
    18301 ccaagtaacg atgtagtgaa tatcatcaat caactggaaa aagacggaaa agtgacacgt
    18361 ccagctctag gtatcacaat ggtcgactta tctgccgttt ctactcagca acaagaacaa
    18421 atcttgaaga ttccttcatc tgtaactaac ggtgtcatcg tgacaaatgt ccaaacagct
    18481 acaccggctg aaaaagctgg attgaaacaa tacgacgtca ttactaagat cgatgacaaa
    18541 gaagtttctt ctggtgtaga acttcaatct gctttgtata agaaaaaagt gggagattct
    18601 gtcaaaatca cttactatga aggctcgaaa gaaaaaacaa ctactgttga attgacgatc
    18661 gatcaatcgg cattaaaaca aaatcaaaat agtgaaaatt aataaaaaag aggttgtgac
    18721 aaagacttgt cacaacctct ttattttgga aatgcttctc cgactacggc atcctcaggc
    18781 aatactaaga ttcctttttt tgctggagca tttggcaaat gcaattcacg agcggaacag
    18841 atcattccat agctatctac ccctcgcaaa gttcctggcc agatcatcat tccatctggc
    18901 atcatagctc ctggttttgc aacgacaact ttttgtccag cttcgatatt gggtgcaccg
    18961 caaacgattt gcaatactgt accattgtct acttctgtct cagtaatcga taaatggtca
    19021 gaatcaggat gtgggacgca tgtttttacg tacccgacaa cgatcttcgg ttcatgatca
    19081 gcttcaagct gttcttcgaa tcctgcttct ttgatcaatc gattcaattt cgctaattcc
    19141 tcatctgtta agcttacttg gccgttccct tcgattgtta gatgatcgga gatatggaaa
    19201 aagttccaag caacaacttg accttgttca tctttgatcc gagcaacatt ccatttgcgt
    19261 tcaacagtgt tttctgttcc tttatcgtcg gctataataa ccatcaaggt gtctccgacg
    19321 tgtgctttgt tataagcaaa aatcatgttc tttcctccat tatcctttgc tgagatttat
    19381 cttaacaaaa aattgcattc actgcatgaa atttttttca tgctttattt ccgaagaagc
    19441 ctcttctgga acaccattta ttcgtaacaa gggactgtga caagactttt gtcacagtcc
    19501 ctttactgtt ttagttaatt gattaaaaag gtcgtacaag ttccacttgc ttaattactt
    19561 ggctgttctt cttcgtcaga aacgatcaag acatcacaag gtgaatgacg tatcacgtag
    19621 ctggctacac ttcctgtcat aaagcgttcg actgcattta gacctgactg cccaaccatg
    19681 attagatcga tctcatattt ttttggcaat ttgactgcca tggattcacg aacagaacca
    19741 tatgcaataa ttccttctat atttgtgtaa tcaacagatt tcccatattc tttacattct
    19801 tcgatcattt ccttggctgt ttcagtttgc tgatcgacta gattttgatc aataggtgca
    19861 tagcccataa agttataaaa ttgctgatcg attacgtttg ctacatatac tttcccatta
    19921 ttacggcgag ccacttctac cgcttttttg aaagcagcaa gtgcttgttc actaccgtcg
    19981 atacctacta aaatattctt atattcttga ttcatcttga cccctcctaa cagatgtcag
    20041 ccttttgtta cactttctag gaacgattcg atttcctgtt ttgttttacg atctttattc
    20101 actagacgac ctaattcttt tccgtcttgg atcacgacaa aacttgggat accaaaaatg
    20161 ttccattcag cggctacatc tatgtacttg tcacggtcta cttcaataaa ttgccaatca
    20221 gaaaagtcat tttcaatttc aggcatttgc ggtttgataa aacggcagtc tccgcaccag
    20281 ccggcagtaa agaaaaatac actctttcca gtttctacgt agcctgctaa ttcttctaaa
    20341 ctatttggga taatcattct ttgtcctcta ctttctgttt atgtattttt caattaaatt
    20401 atacaccttt ctttcgaact aacaaacatt gatcctcttt ttataaaaaa aatctggcaa
    20461 aaagcaatct tttgccagat tttatagttt cactatcgca ttattttccg tatacgatcg
    20521 tatttactgt tgatttatcc aaacctttca gtgtttgaat caacatttct cgtgcagctt
    20581 caaaatcgcg aatactgaac atcgtttgat gcgtatggat ataacgtccc actacgccaa
    20641 tcactgtact tgggattcct tcgttttgtg tatgagcagc acctgcatct gtaccgcctt
    20701 ttgaaacaaa gtattgataa gggatattgt ttgtttcagc gatatccagc aagtactcgc
    20761 gcaaacgagg aagcatgatc aaacctggat cttggatacg catcaatgta ccttctccta
    20821 aatgtccgaa tgtgccattt ttcgtcactg tatcatctgc tgcagagcag tctactgcaa
    20881 agaacagatc tggcttgaat ttattgacag aagctttaga accacgcaag cctacttctt
    20941 cctggacatt cgcgcctgca atcaatgtat gacctaatgt ttcattttgc aacgcttcta
    21001 aagcttctaa taccattgta cagccatagc ggttgtccca tgatttactg ataatattct
    21061 ttccgttggc tgttttgatc gtttcagcgt aaggaacgat tgtgtctcct ggaaggacac
    21121 cgtaactcat tgcctcttct cttgattcaa acccagcatc gaacaaaata tcggttactt
    21181 ctactgattt ttgtccgctt gttcctcgca ataaatgtgg gggaacggaa gaagaaatac
    21241 atggataatt cttaccagta gaggttttaa gtgtaaaacg ctgagcagaa acaacgtaag
    21301 gattccagcc gcctagaggt acgactttaa aaagcccgtt ttcttggatt tgtgtcagca
    21361 tgaatccgac ttcatccata tgtgctgcga ccattacacg gggtgcatcc gcttcttcat
    21421 gatggcgcag accaaaaatg ccgcccaaac catctaactg taactcatct accagcggtt
    21481 ccatatgact ttccatataa gcgcgaacat cttgttcaaa accgcttgtt ccttgtaatt
    21541 ctgttaactc tttgattcgt tgaaatgttt tttcttccat atgctattcg tctaaatttc
    21601 agactaaact cctctcagac ttatattgat ctgcggataa aatatgattt tttctgcaga
    21661 ccgatcgtat ttattatagc ttattttttc tggtagcggt tatttatttc aaagaaaata
    21721 tgagagtgtg gtaaaataag cacaaggagt aacaagtgag aagaaggagg tatgtccaca
    21781 tgtatgaaga aagcgaacta agctatttta aaggcgggtt agcagttgga atcggtctag
    21841 gattattgag cggggtagct tcaacgattt ggtatcatcg caaaaaagta ttgtcagcag
    21901 accaagtact tgaaaatatc aagtcatctt ttctaaaaga aggtgcaatc gaaggttctt
    21961 ggatctcttt tgaaaaggaa cctattagga aattcgccgt tcggtcgagt gcttatcggg
    22021 gaggtctttc tcgaatagaa gatggcgaaa ctgtttatta tgaattttta gcagatgctc
    22081 atacaggaac cgtattagaa atttcaagaa aaaaaggtgc aacagattaa tacgtcaaaa
    22141 aagtgagaga acgaatacgc acccactatt caaaagagag gtatcatgtc cttcttttgt
    22201 agtgggtggt tttttagata gagctttcca aacatatatt ccagtatttt ctttttaaat
    22261 aaatgatatt tagcgggaaa aactagcatt tcctttgatt atgaggtatt tttaatttag
    22321 cttttgtttt ctatagttaa ggagagtgaa aaatggaaca acaagaaaaa attgctgttc
    22381 tgattccttg ttacaatgag gaagcaacaa tttcaacagt cattgcagac tttaaacgag
    22441 aattaccaga agcagatatt tatgtctatg ataataattc gaccgatcga acatacgaat
    22501 tggccgttca aggtggagca atcgtaaaaa aagaacctcg tcaaggaaaa ggaaatgtga
    22561 tccgccaaat gttctttgat atcgatgccg actattattt aatggtagat ggcgatgata
    22621 cttatcctgc agaagctgta catggtttgc tcgagaaatt acgttctggt gaagcagata
    22681 tggtcattgg tgatcgccta tcaaatggaa cctactttga tgagaataag agacctttcc
    22741 acgattttgg aaataacctt gttagaaata caattactag aatgtataaa acaaaaattc
    22801 ttgatgtcat gaccggctat cgaggattca atcgtatctt tgtcaaaagt tttcctatta
    22861 tgagttctgg ttttcaaatc gaaacggaat tgacgatcca tgctttagat aaaaaattca
    22921 agttcgttga aatcccaatc gattatcgtg atcgtcctga aggcagcgaa tcaaaattga
    22981 atacattctc tgacggattc aaagtgatca tgatgattat taaaatgttt aaagattata
    23041 agcctttgct tttctttggg atttggacca tcttcttctt tttatttgga ttggtcacgg
    23101 ggattcctgt gattcgcgaa tatatgctga caagctttat tacacgcatc ccttctgcga
    23161 ttttaagtac tggattgatg actttagcac ttctttcatt agtaacaggg ttgattttgg
    23221 atacagtcgt cacgaacgct aagaaagaat atgaattgaa tctatatcat gtttatcatg
    23281 aataccacag tagaaagtaa aaaaaggatc aagactgcaa aaataatttg caattcttga
    23341 tcctcttttt aattaattat ttcgccaaac gatagatggc atctgcatag attgctgttg
    23401 ctctgaacaa gtcatcaaga ctcataaatt cgtttgcttg atgcattgta tcagtatatc
    23461 ctgggaacat cgctccataa gcaacgccac gtttcaacaa acgtccgtaa gttccgccac
    23521 cgatgatttg ttcgtagcct ttttctccag tgtggtcttc atagacttgc aataatgtag
    23581 ccaccaatgg atcatccatt ggtacgtaat gtggttccat tacgcgagca cctcgagtaa
    23641 ctgttgcgcc ttcttgcccc actgtttgtt cgatgtcact ttgcaatccg tctactgtta
    23701 caccttttgg aaaacggaaa ttcatattga tgaagttcgc ttcctctgta ccattttctt
    23761 caaaagcaaa tagtccagca ttcattgtca atttgcccat tttttcatct tcataagcga
    23821 ctcctagttt ttctccataa aaatcttcat ggacataagc agctgctaca tggataaatt
    23881 gtttagcaga tcctaggaat tcatagttat ctaagaacaa tgccaagaaa gaacctgcat
    23941 tgataccaga ttgtgggctt gcaccatgtg cgcctttacc aactaattcg atttttacat
    24001 acgtattgtc cgcttcaatc gttccgctta ctggatttgc ttcgatgaat tggtaaaatg
    24061 cttcttccat cgcgatagct gcatctgcac ttggtacttg aagtgctgca gttgctgttc
    24121 ctggcaccat attttcgcga agtccggaaa caaaagattt caagacataa tctcctgcat
    24181 tgtcgccttt gaagcgtaaa gctaaagaaa catttccttt ttcaccattg atgattggga
    24241 attcagcatc tggtgagaaa ccaaaatctg gttcttcttc atgttcgaaa taataagcca
    24301 tatcgcccca gccgctttct tcatcgctgc cgactacgaa gcgaactttt tttgatagtt
    24361 tcaagtcaag ttctttaatg atcttcatcg catagtaagc tgccatactt ggacctttgt
    24421 catcgcttga tccgcgtgca aagatctttc catccttgat tactggttca taaggatctg
    24481 tgtcccagcc atctcctgca ggaacgacat ccatgtgtcc aaatatccct agcgtttcgt
    24541 ctccttcgcc aagatcaata tgccctgcat agttatcgac attttttact ttaaatccat
    24601 cacgttcgcc ataagctaac atatgtttca atgcgtcgcg aggtccaggt ccaaatggtg
    24661 catctggtgt tacttttgag tcatcgcgct cgctgttgac acgtaaaaga tttttcaagt
    24721 cttcaagcaa gtcttcttta cgtgcttcta cttctttttg ccaatcaatt gtcataaagt
    24781 taatcctcca cttatttaag tcttcgtgtt catgatacca caaattacag aaaaaaatag
    24841 ttcaaaaaat ctgacaattc cttttcttat ctgtttttct ctaaaaaaaa tatacgctat
    24901 aatgagtaaa aggaggtttt tagatgctga taaacgaaca actagtgaaa agagtcatca
    24961 cacctcgtcc aaaaatttca cataaaggaa catttggacg tatcgttttg attggcggca
    25021 atgaacaata tggcggagca atcattatga gtgctttagc tgccgtacac agcggagcgg
    25081 gtctcacgac tgtagtaact gatgaaaaaa atcggagtgc tcttcacagt catctgccag
    25141 aagcaatgat tgtagtttgg acggatactg accagtttct ttccgtaata aagcaagcag
    25201 atgtactctt gattggtccc ggattgggta cttcttctaa gagtttggaa cgtttcaaac
    25261 aagtactaga aaaccaatca aaagaacaat ggctggtgat tgatggctct gctttaagtt
    25321 tacttgctgc taatcatctt ttgctaccct atcccgaaca aactgtactt acacctcatc
    25381 aaatggagtg gcaacggttg agttctctac cgatacaaga acaacgggat aaagaaaaca
    25441 aaaagaagca acaggaaatc ggtgctgtgc tcgttttgaa aagtcatgag acaaaaattt
    25501 atggtcacca gcacttctac accaatcctt taggttctgc tgctatggca acgggcggca
    25561 tgggtgacac tttagctggg atgatcacag gatttttagc ccagtttcct caaaatgacc
    25621 aaacgattgc agcagctatt tatcttcata gtttcattgg agaccagctg gcaaaagaac
    25681 aatatattgt tttgccgaca caaatcagcg aagcacttcc gaaatggatg aattattttt
    25741 gtaaaaaatc aatggaaaac gaggcatgag tcatggagaa atttacaggt tatgtgcgag
    25801 aaccttttta ttatgaaaca gatcaaatgg ggattattca tcacagcaat tatatccgct
    25861 ggtttgaaga ggctcgggta gccttattgg tacatctagg gtttccctat aaaaaaatcg
    25921 aagaatctgg gatcatcatc cccgttctgg aagtaaattg cagctataaa gaaatgatcc
    25981 attacggaga aaaagtgagc atccatccaa ctatccaaaa gtatacgggg acacggttgg
    26041 attttagcta cgaaattgtg ggaactgaag acaaaaaatt aaaaactact ggcagtagta
    26101 aacactgttt tttactttct gattcgcaac gattagtgaa gctgagcaaa gtcaatccgg
    26161 agttagatca gttattccaa gcgtacgctt ctttggatga tgtaaattct taataaagac
    26221 cgtctaagca tttttcttgt attgtgtcga gtagttcgta taatgaaaag aaaaaagcag
    26281 gtgatcccca tgtatgtcgt taaagtaatg cacggttaca tcgataaaac tggctgtcgc
    26341 actcgggaaa aaaacttaga caatctgctc attttcaaag acaaaaaaga atccgaagct
    26401 tttgctaaaa gaatcggcgg tcgtgtcaaa ccgatccaag aggtacgacc agattgattc
    26461 aatatttttc ttttcgaggc tctaataggc atgcctatca gggctttttt tgcagactgt
    26521 gtttttatgc ccacacgatt tgtaagcgat ttatgttata atagattata tctgcataga
    26581 aaggaaggtt aaatggttat caataatttg caacagcaag cttatgaatc tatacgtaaa
    26641 caaatcattt attccgagtt agcacctgga agtaaaatct ccgataaagt gttagaagaa
    26701 caactagcta tcaggcgtac accgattcga gaagcactga tccaactgcg caatcaagag
    26761 cttgtaaaaa ccattccaca gtccggcact tatgtttcaa aaatcgatat ccgatctgct
    26821 tcattagccc gttatacgcg ggaaaagttg gaaaaaccga tcctgcaaga atgcagtgcg
    26881 aaaatgacca agcaagatca agctaaacta gaaaaaatac tagaacaaac cgatcaggca
    26941 gtaatcgctc aaaataaaaa agcttttttt gatttggata ctgattttca ccgaacttgt
    27001 tatgaaattg ctggaaaacg agaaatttgg gattggctag aaagttatag tacgcacttg
    27061 aaccgtttca gatggttacg tttgacgatt tcagaattag actggggtcg agtattagat
    27121 gaacatcaga caatgcttca atcaatgatt gatcataact ttgatgaagt cggatttctt
    27181 tgcacgatgc atttacatat gattatcgag gagcaagaat acgtgattca taattacccc
    27241 gattattttg aagatattca ggacagaccc atcaaataag aggttttgga atcagcgttt
    27301 tgacctgagt attagagaaa gaaacggcaa aatagttcct catattttgc cgtttttttt
    27361 gagctaatac cgagggtcag ctgattgcac accgtttatt gagaggttgt gaaaaagctg
    27421 ccctgcatca agtaataaga acagccaagt aaaaatagct tcttatgttt ttaacttggc
    27481 tggacttatt accgcagatg caagcttttg aacaccgttt tccaagaggt cgtgagcctg
    27541 ccgttcatct ccgagtcaca aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat
    27601 attttgactt gtgaccgagg agttgacagg agaacaccgt ttattcggaa aaaaaggact
    27661 gtgacacgac ttttgtcaca gtctctttat ttgtaatcta aatcatactc cgtacatctt
    27721 ttttctattt tttttatttc ttctaagatc ccttggctac gaatttcatt atatggccaa
    27781 agtaagactg cctctaatac ataaggcgaa aaatcgcttt gattcatctc ttttgaaaaa
    27841 ctcatcgctg ctatctcgct caatgcacga atgtttgatt gatgtgtata aaaaggcaac
    27901 cgccacagca caattttttc gctttgcgta taaattccct taacagaagc ttcgatgtgg
    27961 tgaaatagtt catgtccaat aatcaaagct tgcgctgttt ctttagagat cgaagctagt
    28021 cctgaacatt ttaatttttc taacggctcc gtcatcaatc gaatcccttc atcttctgca
    28081 taagttgcaa aagtgattcg ttcgtttatt tgctctgatt cacaggaaac gattggaaca
    28141 gccatttgtt cacaaatttc tcctacatcg gcaaaagggt actttttttt gatgctacat
    28201 gccgtttcga taccacaatc gattgattgt tgtataagaa ttttctgctt ggacaaagga
    28261 atctttttcc aaaataaatc tcgccgaaac tggtagaatc cccaaatttc ttcggggatc
    28321 tgttgtaatt cgtttagtaa ctcttctaac ttatgtttgt ttgatatttc cggcaacgac
    28381 gatcacttct tcctccggtt ccatagtaga tacttccaga tccatcaaaa gctgtagttg
    28441 ttccagatcc gtcgctgtaa aatcagagac tcttgctcct aagcagagat aaaattctgg
    28501 tcgggcaatc aattcagttt gataaactgc catatcttcc caaagttgtt cgacagctgt
    28561 catataattg gctgcagttg ttttcataca tgaagcatgt ccacgttgga ccttgataaa
    28621 tcctttttga tcgatgatcc gaagacttcc agcatttttt gaacgattct gttcgccgca
    28681 tacataaaag aaaggtgtat tttccaataa gcaaacttca tttttattta atcgcatatc
    28741 ctctgcagca agctctagtg cttcttctgt tgtgatctcc ttggttagat cggttgcttt
    28801 cacttccgta gaaccagtag cgatagccgt tacttttgaa gtctgtggat cgatttctac
    28861 gtggacttcg atcgattctg gtgtagcgcc tgattcaatc gctttattca tcgcttcatt
    28921 tttcaacgat cgtatatctt ctttacttgg cgaagggatg atacgttcta caacatctcg
    28981 gaccattgca agtgctacac caatagaaga aatcacttcg gcgttttcag gaatcgaata
    29041 tttcacaccc attttattac taaagtaagt aatcaatgag gcagcgccgc cccctacacc
    29101 tacaagagaa atctgatctt tctccaagtg atacttgtct gccaattcta aaatcaccgg
    29161 ttcaatctta gcataggatt tttccatgat ctgttctgcg atgtcttcaa ccgtcgtatg
    29221 acaataatca gctaatgctt ggatggcttt tcgtgcagat gggacatttc cataagaaaa
    29281 atgttcttct tgcactaatc caaggacatt ggctgcacat gtatttgtaa tagtcacttc
    29341 ttctccgtct tccatcacta ccttgacgta atcagcaggg tctccttctt taggagagaa
    29401 aaattcaacc ttaggacctt tgattttttc tgtctccgta aatactgcat aatctagacc
    29461 ggcaatatgt gcagaacgtg gaccgacatc gatgattcct gactgattag cccgtaccat
    29521 cgaaccgcct gccacaccta gaacccgaac atctaatgaa gagatatatg ttgggtggcc
    29581 acctacaatt gaataatcga ttgctggacg tccattttta atgaccccga tattggttgt
    29641 tgttccgcct acttcaaaat aaacaccatt agacgctctc aagtacatca aagagcccat
    29701 gactgatgct gctggaccgg aaagcatggt taaaacaggt cgttttttca tttcattgat
    29761 ttccattact ccgccgtctc cgcgcataat catcaaagaa acattgaccc ctgcttctcg
    29821 tacggaatct tctgtagagg ttgccgtatc tagcattttt ggtaaaatag atgcattgat
    29881 tgctgctgtc cgagttcttc ttgtcaatcc atataatttt gtgatgtcag aagccatcgt
    29941 tgtagggatc gattttaccg atgctgcttc ataaacaact cgttctggtt ccccattatc
    30001 tactccaaat gccatagaag aaaccaagac ctccgctctt tctctttcta aagaagaaat
    30061 cgtttctgaa acacggtcta catttaagtg tttgacaggt aagaatgcat tgacaatctc
    30121 tatttttttc ttattcccta aatcgatatc gttcaatcta gtttgtcgtt ttgctaaaaa
    30181 accttctaat ccaccttttg ccatcccgat tacgccgact tttgctacgt ctccttcaat
    30241 cagtgcatta gtcgcttgtg tggtagaatg tgcaacaaac acaacatctt ctggactgat
    30301 attattttcc agcaaacaat tttgaaaaga ctgaacgaca cctgcggcta ctccacggac
    30361 atcatcgtgg gttgttttca ctgaagattt tccgatgatt tcgtgcgtcg cattatcgat
    30421 tgcgactgct ttcgtatgtg taccgccaac atcaattccc attcgcacaa aacgtttcat
    30481 tttcttttcc tccattcatc taatcgtttt tatcgacgtt ttgtttacta ggcgttcccg
    30541 tacaataaga acgtgatcag gcataagatc gctccgatga tccaaccagt tgggatggat
    30601 ttcttcatat attctcgaga agttactttt gtataaccta atccccaagc aacccaagat
    30661 tgtgtaatat ctaaatgctg aggagcaatc gttgtgattg caaataacgg gaacaaaaag
    30721 gctggcgaca tcgttggatt aacagctaat acgacagcta aaatagctga gcctgaaccg
    30781 actagattca ttggaccgcg gaaaaatcct agtggtgtca atatagcaaa tacgattgcc
    30841 aagactaatg gtgattgcgg tatccaatct ccaaaaatag ctgaaaaata aggagaagca
    30901 tacgcagctg aattattgaa catcgctaag gtcatcaaga aaccaaccat tggcgcaaca
    30961 tctactgcgc catctgtaaa taatttcgca aactgacgac agatttcttt ataacctccg
    31021 cgcaattttc cagtaaacag caatgcccag atacctgcta gaataaaacc taagatgatt
    31081 ggaagatcta aaagaacgac ccctaaaact ggtaataata cggctaacca tgagatcatt
    31141 ggtgcgtcgc caccttcagc tggtacgttt gctgccattg catagcgttt ctttttgttc
    31201 attgatatat tagcgaccgt tagtaccaca actaagctta caatcatccc aatcatacca
    31261 atttggaaat agtcattata agtatagctt tcagccgctg gattgaaacc agcataaatc
    31321 gtttgatatt gtttgaaatt gacgatatta gcaaagattc cagccataat cgagcccata
    31381 aatgagaaca gtgcaaccgg tactgaaata cctaatgaca tcaagattgg caacacaata
    31441 actgcaatag aaatcactgg tccgattcct gtcatcgaca tgaatataac agcggttaca
    31501 atacatagta agctcatcgt gattcttggc ttgtcccctc caagttcaac tacctttcga
    31561 atcagggtag ctgcgattcc gctatctact agaacacgcc cgaaaaaggc accaaagaag
    31621 acatttacca agatcgattt ggcgtactca gctggccccg tcgtatagat atgcgtcaac
    31681 acatcaatta ctccttgccc ttccatcgcc gggtttggtg aaaacgtgtt tccgactagc
    31741 gctaatccca tccatacagt agccatgatg gcaaagccga ccatcaagtt gtagcctttt
    31801 acacagtacc agaccatgaa aaagaatgag ataactaaca aaataccaat aataatatca
    31861 ctcatgttat tcctccgtcc attttgtttt tctgataagc aaactaatat atcagtcaaa
    31921 ctcattctaa cttatgtagt tagcgctttc attaaagggt gaccataaat gttcagtttt
    31981 caattaaaaa accttaatta tgctatttta gacttatacg ttattgtcct gtttcatttg
    32041 atggaggatt gtaaataaaa aaagaggaaa gaacataact tttcgttatc ttctttcctc
    32101 tttataattg tttagcttat ttagccaaag cttcttttgc ttggtcagcc aatgcagtaa
    32161 atgctgctgc atcgttaaca gccaagtcag ctaacatttt acggttgatg tcgatttcag
    32221 caactttcaa accgtgcatt aattttgagt agctcaagcc attcatacga gctgctgcgt
    32281 tgatacgagc aatccacaat ttacggaagt cgcgtttctt ttgacgacga tcgcgatatg
    32341 cgtagttgta tgaattcatt acttgttctt ttgctgtttt aaataaagtg tgttttgaac
    32401 cgtagtaacc ttttgctaat tttagcactc ttttacgacg tttacgagtt actgttccac
    32461 ctttaacacg tgccatgtat aattcctcct aataaaaata aatcttcaga ttctgttatt
    32521 tgtacttctt atttcatttt tgctagttgt tggcgaatac gtttgaagtc gccactagaa
    32581 accatgcttg ctttacgcaa ttgacggcgt tgtttttttg ttttgccgtg gaaacggtga
    32641 cttgtaaacg cgcggaagcg tttcaatccg cctttaccag tacgtttgaa acgttttgct
    32701 gatccgcggt gtgttttttg ttttggcatg actatatttc ctcctcaaaa tctttcgtta
    32761 tccgactatc ggctgtccaa cagccagtta gtcattcaaa ttacttactg tcgtttttcg
    32821 gtgccagcgt caagaacatg ctgcgtccgt ccattttcgc tttttgttcc actgttgcaa
    32881 tatccgcagt ttcttcagct aagcgattca agactttttg accaatttct ttatgggtaa
    32941 tggcacggcc tttgaaacgg atagaagctt tcactttatc gcccttttcc aagaacttac
    33001 gtgcattacg aagttttgta ttgaagtcat tcaagtcgat cgtaggactt aagcgaactt
    33061 cttttacatt gatcactttt tgttttttac gcgcttcgcg gtctttcttt tgttgttcga
    33121 aacggtattt accgtagtcc atgattcgcg caactggtgg tttcgcacca ggggcaacta
    33181 acacaagatc caagtttgct tgttctgcaa tattcaatgc ttctgctttt gtcttaactc
    33241 ctaattgctc accatctgaa ccgatcaatc gtaactctcg tgcacgaatg ccgtcgttaa
    33301 ccatcatatc ctttgctatg gtcattcacc tccaaatatt atgagagaaa gaaaagctta
    33361 ccgaataaac ggcaataaaa aaacgagttc tcttacagaa cccgcgcgta cttcacctaa
    33421 tagaaccagt gaaaactatc tagcccagtg acattatcaa ctaaggcgag aagcgggtgc
    33481 ttctgctttt atttctcaac catcatagta tagcaaaaac taatcttttt gtcaatgttt
    33541 ttcatacagt ttgtcttcct tttctgaaaa tgattagatt agcagacttt ctagcagtga
    33601 ttcccgcttc tttgctatac taaagaaaaa cgaggtgaac ccacatgaaa aaaatcgaac
    33661 ctattccacc ttctcttcct cttcttacaa agggagagtt ttatttagaa gatgaggaga
    33721 tcttctctgg tcttctgatc aacgggggtg accaaagcta tctcgatgcc aaaaatatgg
    33781 tattaagaga gagtcatatc caaaaactta cgatgcaaaa aacagtttta cgtcattttg
    33841 aatgcagtaa tgtcattttt gaaaaatgtg atttttccaa tctggagtgg attggtggca
    33901 gtttccacca agtcgtgttt catcaatgta aattaacagg tacgaatttt gccgaaagtt
    33961 atcttcgaga ctgtactttc actgattgta ttgcttcgat ggcttccttt agtagtacaa
    34021 acttgaaagc tgtctccttc gatcattgtc aattagaaga cagcgaattt tatgaggtca
    34081 cttggaaaaa cctttttttg tctgacaatc agttgaccgg ttcgaactgg tttcgaactt
    34141 ctctgaaggg attagacttt acgactaatt tcttcacagc aatcgcttta tctcaagatt
    34201 ctttggcggg actaatcgtc aaccaagagc aagcacttgt gatcgcccaa gcccttggtt
    34261 tgcagatcaa agactagatc aacgaatccg ccgccacagt gcaataagca tccaaataga
    34321 taagaccaaa ctgatgatca aggcgattcc ccatccgaaa acggaatggg taaagggtaa
    34381 ctggagattc attccgaaga atcctgtcac aatagttgga acggtcaaga tcagcgacca
    34441 gaccgtcaaa aatttcatcg tgtcattcaa gttgttattc agcaaattat tataagtgcc
    34501 agagagctgg tctaaaatct gcgaagctaa gttcgtcatt tcaactgctt gtcgtgcttc
    34561 gatcagcgca tcgtccaact cttctttttc cttttctgac aacttgcgat aaatagccaa
    34621 tgccttgatt tgttctaaaa gtactacatt ttgttttgtt cctgtaacta agtaaaccaa
    34681 accgatctct aaatctgaaa gttgcagcag attcttgttc gttgtttttt ctcttaattt
    34741 cagattaagc cgctgacgtt cactattcac ttcctcgatt aaaggaaaaa aggaatcaga
    34801 aatcaaaaac aatgtacgaa acaataaact gtatacactc atgtctggca ttcgttccaa
    34861 aagcgatcga attagtcgaa tcacgtactc ggtattcggc gtggtaaatg taaacaaacc
    34921 gtcttctttt aaaataaaag cgataggact agtttcataa tgattatcga ctttttgctg
    34981 gtgcggaaca tttgatacaa gcagtaacgc ttcatcaaaa gaatcgtact ctacgcgcgc
    35041 ccgttcattt ttatctagag aataagtcag catctcgtca ctaatgccat attcttcttt
    35101 taagttttta acagctagca catcgttttg ctgtacattg atccaaaaat tacggtcttg
    35161 attaaaatag tgtttttccc gcatactcta tcctcctgac atttgtttcc agtataaaga
    35221 aaaaagtcgc tcaaataaag gtttaccctt tatttgagca actgtaaatc aaatggctac
    35281 tttggtttgg cgaatctctg ctaatttttc ttcgatcatt ttcaagactt cttcttttgc
    35341 ttgaggatct tccacaaaat tatagcggtc tccatcgatt tggatttttg gactttcttt
    35401 gtattcttca taccattgat cgtaacgtct gttcaattct ttgtaataat cgtatagact
    35461 tggatcaaag gacaattgtt catacgaacg accacgtttt tcgatacgct ccaacatcgt
    35521 atcaaaagat acacgaatat gaactaataa atccggatgt tttttatggg cagcataggg
    35581 aagttcttcc atcatgtttt ccagcagttc atcatacaca cgaacttctg tttctgttgc
    35641 tcttcctaga tctgcattta gatggaaaag cagtgaatct tcataaatcg aacggtccaa
    35701 tacattattg tcatctttca ttgcttgttt tatgcttgca aaacgtttat tcaaaaaata
    35761 aatttgcagc aaaaaagcat actgttcagg gtttgcataa aataagggca agacttcatt
    35821 atcatcaatc gattcataaa atgcctgact atcaaagtgt tcagccatca tttctgttaa
    35881 acttgatttt ccggcaccaa ttgtacctgc taaaacgatc acgctcattt acctccagct
    35941 cacttcatca ctaaatattg tgtttaactc caaccttttt aaatataccc ccaatatcta
    36001 gtagtgtcaa ttgtattttt ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga
    36061 aaaaagtgtt ttttctttag aaaacgtatt cttagtttta cttctgagat ggttttagta
    36121 aaataaagga ggaatgtttt ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc
    36181 aactcgtttt gggcgcttta ttttttgctt aaaaatcact tttacgtgag aaggagttag
    36241 attatggcaa actatggcca aggagaatca agcggaaaga tcatattgat gggcgagcac
    36301 gcggttgttt atggagaacc agcgattgcc tttcctttct atgcaacaaa agtcaccgca
    36361 ttccttgaag agctggatgc aatggacgat caactggttt cttcctacta ttcaggaaat
    36421 ttagccgaag ctcctcatgc attaaaaaat atcaaaaaat tattcattca cttaaaaaaa
    36481 cagcatgaca tccaaaaaaa cttgcaactg accattgaaa gcacgattcc tgctgaacgt
    36541 ggaatgggat caagcgctgc agtcgccaca gcagtcactc gtgcttttta tgattactta
    36601 gcatttcctt tgtctcgtga aatactatta gaaaatgtcc agctttcgga aaaaatcgcc
    36661 cacggtaatc ctagtggaat cgatgcagcc gctactagca gcttgcagcc gatttatttt
    36721 acaaaagggc atcctttcga ctacttttct ttgaacatcg atgctttttt gattgtcgct
    36781 gatacaggaa tcaaaggaca aacaagagaa gccgtcaaag atgtcgctca cctctttgaa
    36841 acccagcctc atgaaactgg acaaatgatc caaaaattag gatacttgac gaagcaagca
    36901 aaacaagcaa tcattgaaaa ttcaccagaa acgttggcac agacaatgga tgaatcacaa
    36961 tcacttctgg aaaagctgac aatcagcaat gattttctta atctattgat ccaaacagca
    37021 aaagataccg gggcattggg tgccaaatta actggcggtg gacgcggtgg ctgtatgatt
    37081 gcattagcac aaacaaaaac aaaagcccaa gaaatcagtc aagcacttga agatgcgggt
    37141 gctgctgaaa cttggataca aggattagga gtacatacct atgtttaaag gcaaagcacg
    37201 cgcatatacc aacattgctc taatcaaata ttggggtaag aaaaatgaag aactcatcct
    37261 tccaatgaac aacagccttt cgttaacatt ggatgcattt tatacagaga cagaagtcat
    37321 cttttctgat agctatatgg tagatgaatt ttatctagat ggcaccttgc aagacgaaaa
    37381 agcaacaaaa aaagtcagtc agtttcttga cctttttcgt aaagaagccg gcctctcact
    37441 aaaagcttca gtaatcagtc agaatttcgt tcctaccgca gctggattag cctcctctgc
    37501 cagcgggcta gctgctttag caggcgcttg caatactgct cttaagcttg gattagatga
    37561 tctctctctt tcaagatttg ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg
    37621 tggtttcgtc gaatgggaaa aaggccatga cgacttaagt tcttacgcta agccagtccc
    37681 ttccgattct ttcgaagacg atttagcaat ggttttcgtt ttgatcaacg accagaaaaa
    37741 agaagtgtcc agcagaaatg ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg
    37801 ctggttagat tccgttgaag gggatctata tcaattgaaa caagcaatca aaacaaaaga
    37861 tttccaactt ctcggagaaa cgatggaaag aaacggacta aaaatgcatg gaacgacttt
    37921 agctgcccag cctccattca cttactggtc tcctgacagc ttaaaagcga tggatgcggt
    37981 gcgacaattg agaaagcaag gcattccatg ctactttacg atggatgctg gccctaatgt
    38041 caaagtttta gtggaaaaca gccatttatc tgaggtacaa gaaacattta ccaaattatt
    38101 tagcaaagaa caagtaatta ctgcacatgc tggtccagga attgcgatta ttgaataaag
    38161 ggagattttt atgattgaag tatctgcacc aggcaaactc tatattgccg gagaatatgc
    38221 agttgttgaa acaggccatc cagcagttat cgctgcagtc gatcaattcg tgacagtcac
    38281 tgtagaatct gcacgaaaag tcggaagtat ccaatctgca caatatagtg ggatgcctgt
    38341 acgttggaca agacgcaacg gagaattagt tttagatatt cgggaaaatc cttttcatta
    38401 tatccttgct gctattcgct tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc
    38461 cttttatgat ctgaaagtaa caagtgagtt agacagttca aatggccgga aatatggatt
    38521 gggttctagc ggtgccgtga ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt
    38581 gaatttatct cagttggaga ttttcaagat tgcagcacta gccaatttag cagttcaaga
    38641 taatggttcc tgcggcgaca tcgctgctag ctgttatggt ggctggatcg ctttctcaac
    38701 cttcgaccat ccttggctcc aagaacaaga aactcagcat tctatcagtg agttacttgc
    38761 cctggattgg ccaggtctat ccattgagcc attgattgct cctgaagatt tacgtttatt
    38821 gattggttgg acgggtagcc ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc
    38881 gagagaagat aaaatggtgg cttatcagct tttcttaaaa aatagtacag aatgtgtcaa
    38941 cgaaatgatc aaagggttta aagaaaataa tgtaacgttg atccaacaga tgattcgaaa
    39001 aaaccgacaa ttactgcatg atttatctgc aatcactggg gtcgtcatcg aaacgcctgc
    39061 tttgaacaaa ttgtgtaatt tagctgaaca gtatgaagga gccgcaaaat cttctggtgc
    39121 aggtgggggc gattgcggaa tcgtaattgt tgaccagaaa tctggcattc ttcctttaat
    39181 gagtgcatgg gaaaaagcag aaatcactcc actgccgtta catgtctata gcgatcaaag
    39241 aaaggaaaac cgatgaatcg aaaagacgaa catgtttcac tagctaaagc ctttcatgat
    39301 aaacaaaaaa acgaatttga cttcgtacgg atcatccata accctttgcc gcaaatagca
    39361 gtttctgatg tcgatctaag tacacaggca gttgggttta cactaagcag cccattttat
    39421 atcaatgcga tgactggcgg aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc
    39481 gtcgcaagag aagcagattt gatgattgcc actggttcag tcagcgctgc cttgaaagtc
    39541 ccttctttag ccgatactta tacgattatg cgacaagaat atcctcacgg gaaaatcatt
    39601 gccaacatcg gtgcagggac ttctgtcgaa agagccaaag aagctatccg attgtttcac
    39661 gcagatgcct tacagattca tttgaatgca cctcaagagt tggttatgcc tgagggagac
    39721 cgtgatttta ccaattggaa agtacttatt caagaaactc aaacagccat tgacgtcccc
    39781 ctcatcgtaa aagaagtcgg ttttggcatg acaagagaaa cgctcaatga tctagctgct
    39841 ttaggagttc atacagttga tatcagcggt cgaagcggaa caagcttcac ccagatcgaa
    39901 aacgcccgtc gctccaagcg ggaactgagt tatttagctg actgggggca atcgacagtc
    39961 tcttcattgc ttgaagcaaa tgaagcagac acttctatgg aaatcctagc ctccggaggt
    40021 atccgcaatg cctatgacat atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact
    40081 tcaggaaccg tactgacgca tctgatgaat cacggcgtag aagaaactat tatactgatg
    40141 aagcaatggc aagaagagct tcgtctgctt tatactatgg tcggcgcaac gaataccgcg
    40201 gccctacacc aacaatctct gatcttttcc ggaccagtga aggattggtg cgaagcaaga
    40261 gggatcgact tagtgaagta tggaagacgc acagaaaaga gcgtgggaca aaagtaaaga
    40321 acacttttgt ctcacgctct aaacacgtac aaacggcgga agtaaaagca actccttcgg
    40381 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaattcct tcttatttct
    40441 tggagttaaa tgcttctgtc ccagcctctt ttttcgaata aatgatgttt aatcagctgt
    40501 ccttcggtat tcgttcaatc cgctgatttc acaacctctc cactaaacag tgtactaaag
    40561 ctcttttaag gttgttttcc gatataggca agtatgcctc cgtccacata caagacatgg
    40621 ccgttaacga aatcagatgc gctagaagcc aagaagacag atggtccagc caaatcgatt
    40681 ggctcgcccc agcgtgctgc tggcgtacgt ccaacgataa attgatcgaa tggatgacgt
    40741 tcaccgtttt cttgtgtttc gcgtaatgga gcagtttgcg gtgtagcaat ataacctggt
    40801 ccaattccat tacattggat attgtattga ccgtattcag aagcgatgtt tttcgtcagc
    40861 atttttaatc cgccttttgc cgcagcataa gcactgaccg tttcgcgtcc taattcactc
    40921 atcatggagc agatattgat gattttaccg ccgccttttt cgatcatatc cggaataact
    40981 gcttttgaaa cgatgaatgg ggcatttaaa tccacgtcga ttacttggcg gaagtctgcc
    41041 gcatccattt cgatcatcgg tgtccgtttg atgatccctg cattgttgac tagaatatca
    41101 actgaaccta catcttcttt gatctgttga accatcgctt gaaccgcttc ttcatcggtc
    41161 acatcgcaga cataaccttt tgcatcgatg cctgcttctt tataatgcgc tataccttca
    41221 tcaactgatt cttgattcaa gttattaaat acgatttttg ctcctgctgt agctaaagag
    41281 cgtgcaatct caaatccaat accatagaca gcaccggtga ctaatgctac ttttccatct
    41341 aaacgaaaca tatccatact aaattccatc attttctctc ccttatgctt attttaattg
    41401 atccatttgc accatatcca tgtctgtata ggtgatattt tctccgcaca ttgcccagat
    41461 gaatgagtaa ttgcttgtac ctacacctga atggatcgac cagcttggcg aaataatggc
    41521 ttgttcattg tctacaacta aatgttttgt ttcatctggt ttgcccatca tgtggaacac
    41581 acgtgtatct tcattggcat aatcaaagta gacataagct tccatacgac gttcatgtgt
    41641 atgacaaggc atcgtattcc aagcgcttcc tggttctaaa atcgtataac ccatttgtaa
    41701 ttgacagctt tcgcatacat ttggatggat gtattggtag attttacgtt cattcagtgt
    41761 caggccttca ccagtctcca ttggttttac ttggtcgata ctgattttta catttggata
    41821 tttatgatga gctggaacag aactaatata gaatttagct gggttttcag catcttgaga
    41881 agaaaataca acatgtcgtg tttcttttcc gatatagtat ccgtcttgtt ttttcatttc
    41941 ttctttttgc ccatcgatct cgatgaatcc tggtccaccg atattgatca cacctagttc
    42001 acgacgctct aagaagtaat caacacctag ttctttgtct aatgtgattt ctaattcttt
    42061 ttcagtaggt gtcactccac caaagatcat tcgatcgttg tgcgtataag tcaaactgat
    42121 tttccctggt tcaaatacct tttctactaa aaattctttt ctcaattgtt ccgttgaata
    42181 atgacggata tcttctggac tgtgtgtata acgtgtttcc atttcttgca ttttcttcta
    42241 ctccttctat cgaatgattt ttccagagcc gcatgctaaa aattgtgcga cttcttcttg
    42301 actaaattgg ttgcagtccc catggatcgt atgcttcaag gcagaagctg ccgtagcaaa
    42361 atcaatgatt tcttgcggag acatattgga taaaatccca tggagcacgc cacctgcaaa
    42421 agcatctccg ccacctactc gatccacgat tggggtgatt ctatggactt gggattccac
    42481 atattcatgg ttcatccaca atgtcccctt caattcattt tcacttgctg aatgaacggt
    42541 acgcttggtc gaataaaaca cctgaatatt cgggaacttt tcctgcattt tttgataata
    42601 ataaattaga ggttgttctt ctgcttctgg cgctttctcg atacctaaca aataaagagc
    42661 atccattttt ccagctgagc agatatctac taaaggtaaa agctgatgga ttacttcgct
    42721 ggcttcttgc tgtgtccata attttccacg atagttgata tcgaagctga tcaggcagcc
    42781 agcttttttt gcagcttcta tcacctgttt cgtcattttt tgccattttt tagacaaagc
    42841 tggggtgatt cctgaaacat gaaatatatc tattccttgg aataactcct ctagcgacca
    42901 ttccaaatga tccatttcag caaaacttga tccggcacga tcatagatca ctgaggcggc
    42961 gcgttcgcca ctaccagttt ccatataata agtacctagt cttgatccac cagataatac
    43021 ctgtttcgta tctactccat aacattgcaa gtgctttttg actgcttttc ccaatgcgtt
    43081 atcaggtact tttgtagcaa aaatcgcttc atgtccgtaa ttagctaaag aaattgctac
    43141 gtttgcttcc cctccgccat aatgggcagc gaatcgttca cttgaatcga tacgggttcc
    43201 ctgctctgtg gacaatcgca acatgatttc tccgagtgtc actacttttc ccataagcag
    43261 ctacctcttg atttctttca tttttgctgc atactgttgc gcgacttccg ttactttgtc
    43321 aaaatctcct gtagctgcgg gtgctaaaag gttcccacct actccgactg ttacgacacc
    43381 agcatcgaac cactctttca tattgtccag actcacaccg ccagttggca tgatattgac
    43441 ttgaggcatc ggcgccttga aggcagaaat gatacttggt ccataagcgc ttccaggaaa
    43501 tagtttgacg atatctaccc cgcttttcag cgctgttttg atctcggtaa tagtcataca
    43561 tcctggtaag taaggaattt gatacagatt gcacatctct gccgtttctt gatcaaagga
    43621 agggctaaca atatactctg cgccagctaa gatagccaag cgagctgtga cagcgtctaa
    43681 tactgtgcct gcgccaatca ctatttctgg ttggtctttg tagaatgaca aaagttcttt
    43741 gattacttga tctgcttgag ggactgtaaa agtcaactca ataccagtta gaccaccttt
    43801 gatgattgcg tggcaagcat tcaaagcttc ttctttgctt tttccgcgta ccaccgcaat
    43861 cactccggcg tttttcaatc gagataaaat atcaattttt ttcatcttct ccacctattt
    43921 cctatttaga aattttattt tataacttgg aaatctatac ccatattata cggcagaaaa
    43981 gaaaaattgc aaacgctttt ttcattattt ttgaattctt tgctcgattt cgctttttgt
    44041 ttgcttgagg gcactgatta tttgttcttt tgtctcatct gttaaacgat atttcggcat
    44101 acttacacta aatgcaccaa tgatttgttc gtttttcacg attgaggctc ctatacaaaa
    44161 aatatcacgt tcgatttctt catcgtcaaa tgcgatgtgg gtttctttta ccgttttgat
    44221 ttcctttttc aaccgtaatg cattggtgat cgtatattct gtgtaaggga tcaacggatg
    44281 cttttcaaga taatcagtgt actgctcttc actgaattct gctaatacgg ctttgcccat
    44341 cgcagaattg tataatggtc gggtgattcc gatttttgaa gacatgcgga tcgtttgatg
    44401 ttttggatct agtttgttca cgtacaagat ctcattgttg tccaataccc ctaagtggat
    44461 agtctcatcg atcttttgct gcagctgttc taaaaacggt aaagtcaact ctaccaaatc
    44521 gatttgttca atgtttttat tcgcgtagcg aataagcttt gcacctaaac gatagttttt
    44581 ctctgaattt ttatttacgt agccgatcat caacaaagta tctagtattt tcaagactgt
    44641 agaagaggtc attcccacac cctttgcgat cgtctgcaaa gagacatctt ggttttccgc
    44701 taaatagtct agtattttag aggctttgat caatacagtt ccataaggct ttttattttc
    44761 catataatat tctcctattt ctttataaga aaaattatag cggaaatacc atcaatttaa
    44821 aagagatatc cagatataaa ctaccatttc tctttacagg caatttaatg ttgaagatcc
    44881 gcttcgtttt tacatcaaac tttgatataa agcaatgatt tcttctactg tcgtttctct
    44941 tggatttcct ggtgtgcata catctcgcaa agcatcttct gcaattgctg gaatatctgc
    45001 ttcttttaca cctaattcag aaatggtttc tgggattcca accgctttgc tcaaccgatc
    45061 gatctcacca caagccgctt ctcttacatc ttctaaagac atttcagctg ctcctttaat
    45121 ccctagaaca agagcgatct cacggtactt ttctccagta tattctttgt tatatttcat
    45181 gacagttggc aaaagtgctg cacacgctac gccatgagga atgttgtacc atgcacttaa
    45241 cgggtgagcc atcccgtgta ccagtcctaa accgacattt gagaagccca ttcccgcaat
    45301 atactgtcct aatgccattt tttctcgccc attttgatcg ccatctacag aagcttccaa
    45361 agaatggccg atgatttcga ttgcttttaa atgcagcata tcagtcattt cccaagcgcc
    45421 ttttgtgata aacccttcaa tagcatgcgt catagcatcc atacctgttg atgcagccaa
    45481 ttttttcggc attcccatca tcatatcgct gtcgatgaac gcgacgattg ggatatcatg
    45541 aggatcaaca caaacaaatt ttcggtgatt tacttcatct gtgatgacgt agttgatcgt
    45601 tacttctgct gctgttccag aagtcgttgg tatagctaat ataggtaaac atggattttt
    45661 cgtatcagca acaccttcta aactaatgac gtctgaaaac tctggattcg tcaaaataat
    45721 cccaatggct tttgccgtat cgattggcga acctccaccg attgcgatca aacagtctgc
    45781 ctctgcctct ttaacaaaag ccactccatc ttttacattt tggatcgttg gattaggtac
    45841 gatcccgtcg tataaagcat aagcgattgc tgctttatct agaagttctg ttacttttgt
    45901 tgctataccg tgctcgatca atcctttgtc agtaatgaca gctgctttgc cgaatcctcg
    45961 ctgctgaaat tcagggacga tcgactcaat cgctcctttt ccaaaatacg atgtttcatt
    46021 taaaatcatt cgattactca ttttctattc ctcctaattt gtattatttt tcacaataaa
    46081 aacgaaagaa ccccaagcaa ccggttgccc agttgcttgt tgctacagtt caaagactgg
    46141 catcaattcc caactgactg gagaggcatc tggatttgtc tccatgatat cttccatgta
    46201 cttccaccat ttttgattga tagatgtatt tgccattttt tcccataact tttcatcttc
    46261 gatcactaag taaccaaata acaaacttgt actcggatca agatagatcg aatagtcgat
    46321 gaccccgtgt tcacgaagtg ctgtacgcat ttctggccaa agttcatcat gtcttttttt
    46381 gtattcttct tgtttttctg ggtaaacttt catgcaaaaa gcttttttca ccatgtcaga
    46441 cacctgcttt cgtatgaact tcctccaaat aacctgcttt tggagtgaca ccaaacgcat
    46501 ctgctaaatg ccacaggttt tcgtccgtga ttgtttgcaa gattggtcct tgcgctttga
    46561 cgtacgtata gacttctgct gccttttctg ctgtctcgat caaaccaaag acttcatcca
    46621 tgtcttttcc tgacccgtag attccatgtt gtggccataa aaccaatcga gtttctttca
    46681 ttttttcagc tgttgcttcc ccaatctcat ttgttcccgg aaccatccac ggaatgatgg
    46741 aaattccttc tggaaaaaca actaagcatt ccgtacacat ttgccatagc gtacgactaa
    46801 aggaacgttc gtctaattca tgcgtaaaac tcatcgctaa tagatgacta gcatggcaat
    46861 gcataacgat ccgattatct gggtctactt ctaaacgggc gatatgactc atgaaatgac
    46921 ttggcaactc gcttgtagga aggcattcgt tttccagccc ccacaaaagt tccagtgttt
    46981 tgccatcttc agcaactcga acgattccaa gattctcggc aggttcgctc gctacatttt
    47041 taaaatatct tcctgaacca gttacaataa aatagcatcc tgctaaacgg ctggcatcaa
    47101 aaatcatcga gatgcggcgg atcacttttt caggatccaa gaacggtgtg atctcttctt
    47161 cttttagtaa ataagaaata ttcccgccat ttctttcatc ccatcccaag cgataaagat
    47221 tcgacgtcgt ttcgatcatt tctttcacat aaggcgcttg taaaatatct atttttttca
    47281 atatagccac actcctattt tcttaattcc aacacgtctt tttgatattc ctggacttct
    47341 tccagccaat ccaaaccaac tggaacttga tttttgtaac agaaataatt ccatacatcg
    47401 gcatacggga aatctttcaa ttcttccgta taagccattc ttgaagtaaa gtcaaaagcc
    47461 agctccattt cttccaatcg ctcaactggt tccagcatag ctttcagcag tgctttttgt
    47521 gtatttctcg taccgatgac ccaagcagcg atacggttga tcgttgcatc aaagaaatcc
    47581 agaccgatat ttgttttttc caacaattcg ttacggacta attctttagc gatttcctgc
    47641 aattcatcat ccatcaaaac cacatggtct gaatcccaac gcactggacg ggaaacatgc
    47701 aacatgattc ctttgctgaa taaagctaat gaagataatt tattggaaat cacttctgtt
    47761 ggatgaaaat gtccagcatc tagacaaaca agtttgtcac gagtcaaccc gtaacccata
    47821 taaaattcat gggaaccgac tgtataagct tcagcgccta aaccaaatag tttgctttct
    47881 actgcatctt gtacataccg ttcatctaac tcttctgtaa aaatttcatc gagtgattcc
    47941 attaaacgtt cccgtggggt aaaacgatca atcggattat ctttcatccc atcaggtacc
    48001 caaaagttat tgatacttgt ttgcccagtt tctttcccaa agtactccgc gattttgcgg
    48061 cttcttttcc cgtgttcgat ccagtattca cgaacttctt tatctggatg agccaatgta
    48121 aagccgtctt tcatcattgg atgcgaaaag aaagtcggat tgaagtcaag accgatttct
    48181 tctttttttg cccactctac ccatttttca aaatgtttcg gctcaatttc atttagatcg
    48241 acagattctt ctgtatccaa atagatagca tgaaggttca atttgtgttt gcccggtatc
    48301 aacgaataag ccttctccag atcttgtcta agctcttcag gtgttcgtgc ggctcctgga
    48361 aaattccctg tcgccatgat tccaccggtc aattcgccat ccggtgtcaa gaaacctttg
    48421 acatcatctc cttgccagca atgcatagaa attttgatgt cctgtatctt ttctagtaca
    48481 gcatctgtat ctacaccgat agttgcatat ttttcttttg cttgttcgta atttctttca
    48541 atcgtgttca tacgattact cctttgattt tattcacgta ccattccatc gtgttttgct
    48601 tattttcatc tgggtaaaaa gtttcacatg gaaacgactg tttgattaca tttcttgctt
    48661 gttctatatt cgaaaactga cctgtactga gcatttgcat ggcaacattc ccgattgctg
    48721 tggcttcact aggtcctgct tctacaatga ttccggcata atctgctgtc aattgattca
    48781 gtagacgatt attcgatccg ccccctacga tgtgcagctt ttccaatttc gttccagtta
    48841 agtgttctac tttgatcagc tcttgtgcgt aacaaagagc aagattatca tacacacaac
    48901 gagcgatagc tcccgatgag acagggactg gctgctctgt ttcaaaacag tattgctgaa
    48961 tctcttctat catatttggg ggattcaaaa atcgagggtc attgatttcc atcacttgct
    49021 gcagcggttt ttcttttgca gcaagctctg ccaattccgc ataactgtac tcgtactctt
    49081 gcattcgggc aacttcctgg atcaaccaca tccccatgat gtttttcaaa aaacgcaccg
    49141 tgttatgggc accccattca ttcgtataat tttcagaaaa agcattgcga gaaacattgg
    49201 cgatctttgt ttcaatccct aaaagcgacc atgtcccact gctgatataa ccccaacgtt
    49261 ctccttgacc tggcgttcct aatacagctg acgccgtatc atgacttgct actgtgataa
    49321 aggtcacatc tggcaaatca tattttgaaa attgttcttt ttgcagatct cctagtatcg
    49381 ttcccggctc tgtcaatggt ggaaataatt ccttcgagac accgattttt tcaagaagtt
    49441 ctatttccca ttcttttgtc cctgcattca gcagctgcat agtagatgca ttagttttct
    49501 ccgtcaccat gttaccagta aagacatacc ctaaataatc aggtatcagt aacaatttat
    49561 gtgcttttgc caaattttct ttttcttcag caaacaattg gaacaaggta ttgaaaggct
    49621 ggatctgaat ccctgtctgc tcatacaact tatctagaga atagatttca gaaaactgtt
    49681 ctactgctcc attggtacgc ttgtcccgat aagcaatcgg atcatttatc cgatcccctt
    49741 gttcatcaat cagacagtaa tcgactgccc atgtatcgat ccccgcaaaa aggcgatcaa
    49801 tccctgtctg ctttaatttt tcaaatccaa tcaaaatctc tttgataaga taatcgatct
    49861 tccagcgatc gtgaccgtct ttgttccgaa agccgttttt gaaccggtgt acttcttcca
    49921 agatcaaaga accttttgca tccctcgtac ttttcattag tcgtccgctt gatgcaccga
    49981 tatcgatggc aacaaatgtt ggattcattt ctccacctct tttactttat tgttgattag
    50041 cactttatta ataaagcgtt atcatttatt gacaatgcta tcataataca gaatcttata
    50101 ttttgcttca cactattttg gtatataatt gcattatctt gacaagttgg gagtgtttac
    50161 atggattact gggaaaaagc caaccactca tggtcggaag actcaaaaag agtgatcttg
    50221 acgcctacac aaagaagcag gaatttattt ttttatatcc aagaaatcgg ttacttcaaa
    50281 gcaccaatcc cttactatac tgaacgagca catttacctt cttacttgat aaagtttact
    50341 ttgtctggca aaggttcgct tacatataat gataatcagc aacagctaaa agctggagac
    50401 ttatttttta ttgactgcca agaatatcag cgttatcaaa cgatcagcga agaaccatgg
    50461 gaaatggatt ggatccattt gaatggtcag aacgtccaat cattttatca ggagttcaca
    50521 aaagacggcc ataacacctt ccattcaaca ggagatccat cagaaaacaa gatccaccat
    50581 ttgatcacag aattgatcca catgcaacaa ggcacgaatg cccgtaccga tttccagtcc
    50641 tctgtagcta tccacgagtt gctgaatgaa ctgattttgc aaaagtatca gctagatttc
    50701 gaagaagagg atattcccgc ctacgtttac aaactgaagc aatacttaga cgaacatttc
    50761 aaagaaacga ttacacttga tcaattagaa caacaattct tattgaataa atatcagctg
    50821 agcaaagatt tttccaaatt tatcggttct ccaccaatcg attatttgat cagcaaaaaa
    50881 ctttcttacg ctaaagacct tcttcggtat acagaattta cggttcgaat gatttccgaa
    50941 gaaataggga ttgagaattt tgcttacttt agccgcctgt tcaaagcaaa aacaggacta
    51001 tcgccgagag actttcgtaa attcagctga ttcatataat atgtacgtca ttttttcaaa
    51061 atattttgaa atatatagat aaaaataaaa taaccgcagt ttctgcttct atgtaaacaa
    51121 cagcagctac tgcggttatt ttttagtgat cattctcatt gttcatcttc aaaatgttca
    51181 taatgaacca actgtttctg tgtatcacgt ttcaaaaatt ctttaggcgt tatgccgaac
    51241 tctctcttga atgctttcag aaaatgcgga taagaagaaa aaccactttt actatataca
    51301 gcactagagc tgcggtattc tcgcaacaag tgcttggcat agatcagctt tttcttcatt
    51361 acatactgat gaaacgtaaa tccagtgtac tttttaaatt ctcgtgtgat atggtatttg
    51421 ctgacaaaaa actgcttttc catttcctct aaagaaagcg gatgagataa attttttgag
    51481 atatattgta acatttgttc cacacgctca ttaggaacta cagcattctg ttcttgcgca
    51541 ctctcttctt tttgtacaac ttgattgaga aaaatcaagt attctaccag agcttgttca
    51601 tattttatgt cttcaccgta aattttttta tcaggaggat ggtcgataaa tgataatttt
    51661 tgcttcaata cttcctgttc tatttgcaat acacgactcc tcttctctcc cattggttga
    51721 aaacaagccg atagattaga ccatggcgtg gaacgacttt ctagaaaaga aggggtgaca
    51781 aagatataga cacgctcgaa tacatctgaa gattggcgga cgatacgatg caaatcattg
    51841 gaatggatga gcagcacact tcctgctttg atcgtaaatt gtcgtccgtt caaataaaaa
    51901 gtcgcttctc cctttaatgt agcatggatc tcatagaaat catgatgatg atagatcgtt
    51961 ttattttggc tgacgacatc tttgacatga tagatttcat actgatcaga aaccatcgta
    52021 ttgacttgtt caccgctgat tttcttttgg agcatgtgcc gcacctcctt ttgttcactt
    52081 atgcacaatt ttattatacc acgttttttg taatcaaaaa gagctgcttt tatatactct
    52141 tataaaaaag aagagacgtg gcatcttcta gccccgcccc ctccattcta aatattagtt
    52201 aaccaacgtc ttctttacat tattcaagaa gacttcttcc ataacgaatc ccatcatctt
    52261 ttgtttcttc aagaaccaca tacaggtacg gacgattctt catgatgatt tctttgattt
    52321 ttgtgtactc gatcacaccg tctcccaaat ttaccggcac gattttttcc tgttcgacac
    52381 gaaaatcttt caaatgaacc gcacatatct tttcaccaaa acgttcaaaa gcttcttcta
    52441 ccaattggac ttgttcttga taatcactac ttgtgatcaa attggtcgga tctaaaatga
    52501 ttcctagata gtccgaatct actgcttgga tcagctgctc tacccttgct aaagaataca
    52561 ccggatgatt caaacctggt tctatcccca ccatcatttg gtatttttct ccagctttta
    52621 ccaagcgacg gataacggac acagcttcag caaatgcttc atctgtgaaa ttttcttccg
    52681 tatattggat ttccggcaaa acacatccag tttccgaagc aaccatcgac gcaccaaagg
    52741 aagaagcata gcgaagatac tgctcgaact ttgtcagcaa ctcttgtcgt atcgttaaat
    52801 ctggatgaat catattgata tagcaactta aaattcctac ggtcacttct tgcttttcta
    52861 aaactcgttt gacataattt cccatcccag aattgatctc atttgcgcca gaaggtattt
    52921 caggaaacga aagacctaag gcgagctgca gtgtatgaat cccttgttct tttgtttttt
    52981 ctgcaagatc ttctatcgtt tggacttcgg aaagatcatg tcctctaatt gtaatgttca
    53041 acataactat tctcctaact cagcatattg ggtagccaca tgacgatttg cgggaagtaa
    53101 gtaatcaaaa acaagacgac taagataacc ccataaaaag gaagaagcgg tttcagcatt
    53161 gcctctgctt ttacgcctcc tacacttgca ccgacatata gtcctgtacc tactggcggc
    53221 gtgatggagc ctacacacag attcatgatt gaaaacaacc cgtaatgtac aggatcaacc
    53281 cctacacttg tgacgattgg taaaaatatt ggtgtaaaaa tcaagatagc aggcgccaca
    53341 tccataaaca tacccaccag taataacaca agattgacta ataacagaat aataaattta
    53401 ttatccgtta cgcccataat caacgaacta attgcctgag gaataccggt aaacgccatg
    53461 gcgaaagaca tcatggaaga agtagccaat aagaacatga tcgtccctga cattgctact
    53521 gattgaaaca acatattagg tatatctttt agttttaccg ttttatagta aaacatagca
    53581 atcaacaggg agtaaatgac agctatcgct gaagcttcta ctgctgtaaa tatccccgtc
    53641 aagataccac cgataatgat gacaatcaat agaaggctag gaatcgcctc ttttattatt
    53701 tttccagcat gagccatctg tcctttttcg acaacagggt atttattctt tttagcaacg
    53761 atgaatgcta ctagcatgat tcctagcgcc cacaaagcgc ctacgagata acctcccata
    53821 aacaaagcag agatcgaagc accaccagca acaagtgaat acatgataaa agcagtactt
    53881 ggaggaatga ccatacctgt cggagctgac gcgatattga ccgctgtcgc aaacttccgg
    53941 tcatatcctt cttccacttg ctgagggatc aacactccgc caatcgcggt agaagcggca
    54001 atggccgagc tagagatgga accaaataaa gcattcccta acacattcgt atgagccaaa
    54061 gcccccggca cgcgcccgac gaacaacatc gcaaaatcca ctagtttctt agctatccct
    54121 ccattattca tgataatgcc cgagagaacg aaaaacggaa tagcgatcag cgaaaaacta
    54181 ttgatacttc ccaccatttt ttgagctgaa ctaaaagcag aaacatcaaa cggtataact
    54241 aacaataaag tcatcatcga tgagaatgca atacttatag caatcggcat ccctaataca
    54301 agcaacacaa caaatacaaa aaataacact aatccggctt ctattgccat tttattcctc
    54361 catttttata ttttttcttg aattgctggc ttgttgctta atgtatcgat tagattgatc
    54421 aggctataga taatgatgag tacccccgat acaggtactg acagataaac atatcccata
    54481 ggaatactta aagaaggcga gatctgcccc attgtcaatg tgacagcatt ccatccgcca
    54541 tacaccatga tcatcgcagc aaataccaga aataaacttt gaacaacaac ttctaaaatt
    54601 ttactttttt tgccagttaa tttttcactt aaaaccgtta cggctaaatg tgctttttgt
    54661 cccacaacat acgcggaaga caacatggca aaccagacaa gaccgaatcg aacaagctct
    54721 tccgtgaccg tatttggatt tcctaaaacc gttctggaaa atacttgcca taaaacaaca
    54781 gcgaccatcc caacaagcaa aacagctccc aggatctcta ataccttgtt taaaatcatt
    54841 ttcatcacag ctacgctcct tgctcttgga tcttttgata aaggtctttg taggtaggat
    54901 cgtttttgaa tatatcatga agtggggcaa ctgcctctcg aaacggtgta ttatctacat
    54961 cattgaattg gactccatgt tcttcttgtg ctaaagcttt ttcttcttca attgcttttg
    55021 caaaagcttc aatttcaaaa accgttgatt ctttcgctgc ttcttcaatg gctttttcct
    55081 gctcatcagt tagctgttct ttgatggcat cattcatgat gatgatatct ggcacacgtg
    55141 tatgctcatc ataggaatag tatttagcca caccaccatg tccagctgta tacaagacaa
    55201 attcattgtt ttctgatccg ttgatcaaat tggattgaag agacgtgtac acttcatcac
    55261 ttcccatagg tacgggcgaa ccgcctaaaa gctctaccat tttgatagcg gtttcgcttt
    55321 gcatcacacg gatcttcttt cccttcaaat cttccggcgt atggatcgga ccgtctacca
    55381 tataaaagct gcgctgacca gaatcataat aggtcaatcc ggtaaatcct aaatttttgg
    55441 tcgattggta gattggctcc attatttctt cattatccat caccttaaag aaatgttctt
    55501 catcattgaa tagataagga actgaaaaaa tcgaataatc tttagagaaa ctttccaacg
    55561 ctgaggcact tacttttgtg aaatctactg ctcctgactg tgtcaattcg atcagttcac
    55621 gttctccacc cagttgtcca tctgggaaat actggatctg tacggaacca tctgtttttt
    55681 cttttactaa acgcccaaat tcattcattg catcaattac tggctggctg ttgcttgcat
    55741 aagcaaagcg caaggtaact atctcattta catctgcatc tgcgacagtt gccacactag
    55801 aattatttcc gcagccgctt agacctagca gacctaggct aagcgaactt gctgcaaaca
    55861 gcagtgatgt ttttttcata tgggtttcct ttcttttaac aataagagac gagcaattct
    55921 gtcaaacaaa gcaccgttaa cgactgtcca taaggcattg ctgtgattcc gatattccga
    55981 taaaaatcta aactgtcccc cattcctgtc ccgattgaaa cgttttgaac ttctcctttt
    56041 tcatcgattt gttcaagcaa tccttggata gcacgataag cgacttcttt atattcttgt
    56101 ggtaaataac gtttatggac cgctttcaaa ataccgtaag caaatcctgc agttgcagaa
    56161 gattctacgt aagaagaagg atcatccaac aacgtatgcc ataaaccgct ttcagactga
    56221 taagttttta atgcacgcac ttgtgcttca agtgtttcga tcaataattt gcgcaatgca
    56281 tcttctttcg ataattctaa gatttcaatg atctctggaa tagcaatcgt caaccagcag
    56341 tttccacgag cccaaaatgc ttcggcatag ttatgatttc cttcaaacgt ccagccgtga
    56401 taccacaatc ctgttcgttt atccatcaag tatttgatat ggatcataaa ctggtaacga
    56461 gcttcttcta aatattctgg gcgattcaac agtttcccga tttttgccaa aggaagcact
    56521 gtcatcatca atgtatcgtc ccacaactga ttcttatttt ccggtccata tgtggcatgc
    56581 tgtaacccgt cttcatttgt ccgaggcatg tcattcatga cccattctgc ccattgttct
    56641 aaataaggca aatagcgtga atctttcgtg tcttcataca agtaagccat agtcaatagc
    56701 ggtgccattg tattgacgtt tttgggcgga gcgccttctt ccatacgatc ctcaaaccat
    56761 tcattgacca cttgataagc gcgaggattt tttgtcatct ggtaattttt ataaatacca
    56821 tatagaccga caccttgtgg ccaattccac actgtccagc ttttgtcatc tactttcagt
    56881 ccatcaaagt ccaacaaaaa ttcaccatta tcgtctttga tcgttgtcag gttttcaacg
    56941 agcaggtcaa ttttttcttt cactaattct ttaggtacaa aaatctcttc tttaataatc
    57001 atctttacgc caccttttta aacgctttca ttttatatcc ttaatatagc agtaataccg
    57061 tccactataa atatcgcttt ttgaacaaaa actgtctaaa attgacctaa atgttaaata
    57121 acaaaagtaa ctttcagtta cttggaaatt aaaaaaagaa cttggagaaa caacgttttc
    57181 caagttcttt ttcatctatt ttcttatctt ttttctacgc tttcttttgc ttatccgttg
    57241 taagcatcaa tgatgatctg tttcaattca gagatcaatg gttctttcgg attagctgtc
    57301 gtacattggt cttcgtaagc taattcagcc atacggtcta cggttgtatc tagtgtttct
    57361 tgtgtgactc cttgagcttt tagactcatg ttgatgccga cagattttcc taattcataa
    57421 acagccgttg ctaaagcttc gactaattcc tctgttgttt cgcctttcag acctaagaat
    57481 tttgcgatat ctgcgtaatc tgtatctgca cggaaatagt catatttagg gaacatcgca
    57541 tgtttttgtg gatcttttgc attatagcgg ataatatgcg gtaaaagaat cgcatttgtc
    57601 cgtccatgag ggatcccgta ttccccgccg attttatgtg cgactgagtg acaaatacct
    57661 aggaatgcgt tagcaaacgc cataccagcc attgtagaag cgttgtgcat tttttcacgt
    57721 gattccatat caggtgtttt tactgatttt tctaaatagt caaagactaa cttgattgct
    57781 tgtaggctca atccacgtgt ataatctgaa gccatgactg aaacatagga ttcgattgca
    57841 tgtgttaaca catccatccc ggtatctgct gtgacagatg ctggaactga catgacgaat
    57901 tgaggatcga cgattgcgac atctggcgtc aatgcataat ctgctaacgg atatttcaca
    57961 tgtgtttcac tgtcagtgat gaccgcaaat ggcgttactt cagaaccagt ccctgatgtt
    58021 gtcgggatac atacgaattg tgttttttct ggtttgtcga tcttatatgt ccgtttacgg
    58081 atatccaaga atttttgttt tgcaccaaag aaagatgtgt ctgggtgttc atagaacatc
    58141 cacattccct tagctgcgtc cattgcagaa ccgcctccaa gagcgattac tgtatctggc
    58201 ttgaagtctg ccatcagttt cgttcctgcg tatacggtat ttgtcgatgg gttcggttca
    58261 acatctgaga agacttcgat cttcacatca tttttacgtc gtgccaatac ttcacgaact
    58321 gtatcgcagt aaccgaattg gaccatacca gggtcacaaa cgatcatgac acgttcaacg
    58381 ttttccattt tctctaagta caatagagag ttcttttcaa agaagatttt tggcggtaat
    58441 ttgaaccatt gcatgttatt tctccgtttc gctacagttt tgacattgat taagtttaca
    58501 gcagagacgt tacgagaaac agagttctta ccataagaac cgcatcccaa agtcaatgac
    58561 ggaatcattt cgttatagat atcaccgatg ccgccttctg cagatggtgt attcactaaa
    58621 atacgacaag ctttcattcg aagaccgaat ttgacatgca actcttcgtt ttctgaatgg
    58681 ataactgctg tatgtcctaa tcctccaaga ttcaacatac cttcacacaa atcaaacgca
    58741 tggtccgtgt tgttagcttt gatcatggca agaactggag aaagtttttc tcttgatagc
    58801 ggataatctg ggcctacgcc ttccaattca gcaatcagca ttttcgttcc ttttggtaca
    58861 ctgattcctg ccaattttgc gatttccatc gcagaatgtc caacgattga tggattgacc
    58921 gcatattttc cttcgttcat cactgcatct tctaattttt gcaattcatc tggttttacg
    58981 atataaactt ggtgtgcttg gaattctgct tttactgctg catagatttc tttatccacg
    59041 atcacagctt gttcagaagc acaaatcatt ccattatcaa atgttttcga aacgatcaaa
    59101 tcgttcactg cacgtttaat ttttgctgtt ttttcaatat aagctggtac gttacctgga
    59161 cctacaccta aagctggttt tcctgtagag taagcagact tgaccattcc agctccacct
    59221 gtcgctaaga cgatcgcgat acctggatgg ttcattagca tagaagtcgc ttcgatcgaa
    59281 ggatgttcga tccattggat acaattttca ggagctccgg cagcgatagc agcatcacgt
    59341 accactcgtg ctgcttctgc agaagatttt tgggcacttg gatggaaagc aaaaacgatt
    59401 ggatttcttg ttttcaacgc gatcattgat ttgaaaatcg ttgtggaagt cggattcgtt
    59461 gttggagtta ctccgcaaac cacaccgact ggttcagcaa tttcaatcaa ccctttttgg
    59521 acatcttcat tgatcacacc aactgtttta tcatgtttga tattatgcca aatatattcg
    59581 gacgcataca tatttttgat tgctttatct tcgtaaacac cgcggcctgt ttcctctaca
    59641 gccatcttcg ctaatggcat atgttgatcc aaagctgcca ttgccatttc atgcacgata
    59701 tggtctactt tttcttgatc aaagctttcc atttctttca atgctgcatt tgctttttct
    59761 gccaaatcat caatcattgc ttgtacgtca attgtcttcg ttttttcttt ttctattact
    59821 ttagccatca ttatcctcct aatgggtcat ggttatttgt taaataattc acatgtatat
    59881 catacaatat ttatttttct atgtaaacac ttttttgtga tttttttcac agttattttt
    59941 aaatataaaa ttgctttatt tacaggtgtt tgtatagaaa aatatataaa gttatttatt
    60001 attatttata tgacacaccc gattattcaa tattgagcaa tatatcccat gctcttatct
    60061 ctttcttttt tgaataaaaa atgaggtagg acatctttct gccctacctc gtctttaaat
    60121 aagctttgat acttattttt cttccttctc ttctgtctct tccagcgttt ctgttgtttc
    60181 agtttttact tcaactgtct cagctggttc ttctgttgtt tcaacagtag tcgttgcatc
    60241 atttgttacg acagtccctg gtttgaccgt tttgattgct gcacgatcgt actcaaggaa
    60301 gatcccttcg cagtctaaaa caactgtccg tttgtcattg tcgatttctg aaacaactcc
    60361 atgtaatccg ccgatcgtta cgactgaatc acctggtttc atgctatcta acaagttttg
    60421 acgttcttgc tgttgtttct tttgcgaacg tgacataaaa aaccacattg ctaatagcgc
    60481 gataaacatc acaagcattg gtaatgttcc catttttttc acctcactaa ttctgtatct
    60541 acaaaaaaca ctttagcaga aaaatgctga taacactagt tttttactta atttttaaaa
    60601 gattttcctt tagaagcttt ttgcgttttc tttattgaac ccgtattctt caaaaaaggc
    60661 ttctctaaat tcaagcaaat tatcatccat gattgcttga cgtacttgtt tcattagatt
    60721 caacaagaag tataaattgt ggtaagaagt caaacgaatc ccaaatgttt catcacattt
    60781 gatcaaatgg cggatatagg cacgtgtata gtttttacat gtataacaat cacatttctc
    60841 atcaattgga cggaaatcat gggcatattt cgcatttttc actactagac ggccttgaga
    60901 agtcatacat gtgccatttc gagcgattcg agtaggcaag acacagtcaa acatgtctac
    60961 tccacggata acaccatcga tcaatgaatc agcagcaccg acacccatca agtaacgtgg
    61021 tttgttttcg gggatcaacg gtgtagtgaa ttctaataca cgattcattt ctgatttagg
    61081 ttccccgaca gataacccgc cgattgagta accagggaaa tccatcccga ctagatcttt
    61141 cgcgctttga cgacgaagat cttcaaaccc tgctccttgg atgattccaa ataatccttg
    61201 acggtctgga ttagcatgag ctttcaatcc tcgttctgcc catcgagacg tacgttctac
    61261 tgattttttc acgtagtcgt aactttcatc gaatggtgga cattcgtcaa aactcatcat
    61321 gatatcagag cccaatttat tttggatgtt gattgctttt tctggtgata aaaacatacg
    61381 cgaaccgttc aagtgatttt tgaaatgcac gccttcttct tcgatattcc gcatatcact
    61441 taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc ggttgatccc agttcataaa
    61501 tttatgcagt ccgccagctt cttctactaa gtcttcacca gggcgtagcc ataaatgata
    61561 tgtattactt aaaataacgc cagcacccat ttctttcaac tcttctgggg acattgtttt
    61621 tactgtagct aatgttccta ctggcatgaa cattggtgtg gggaaagttc cgtgtggcgt
    61681 gatcaattcc cctaaacgcg cgccagtatg tttttctttc ttgattaaac gatagcgaat
    61741 ggcaggttgc gtcattcatt tccctacttt ctatataaaa tatcttgaca actcccacca
    61801 tcataatcgt ttcaagtgct ttttgcaagt tccctcggct tccttctact ttatttaata
    61861 ttcttttttt tatccttttt ttatgaataa caaaaaaaag aaaaacatta tgacagaaat
    61921 attttttgat acatggtata ttggtgttat taaagaaaag gagggaaaaa tcatgcaatc
    61981 ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa ggacgttggg gacaagctgt
    62041 tttactaaat ctaatcccta cattgattgg tattgctact attcttctta ttatgattcc
    62101 tattatcttg tttgccacca ccatatatga tggatcagcg agcgtctcag aagcgacaac
    62161 ttcaggaggc ggtagttcag gaggcggtgg cggaattatt tccagcatca ttagcgcttt
    62221 gttcatgagc ggtatttctt ggacttatct tgatatttta cgtggacaaa aagatcagat
    62281 cgaaccattc aaagatgcat ttcgtggatt caaaggtttg tttatcggcg gagtgatcag
    62341 cttagctgtg ctgatctcga tctttacaac attatggggt ttgctattag ttattcctgg
    62401 aatcattaaa agctattctt attctcaaag ctattttatt tactacgata tcgtccaaga
    62461 aacaggacaa acacctaaag tactggatac gatcactgca agtcgaaaat tgatggatgg
    62521 ttataaagga caacttttct ggttagatct ttcatttatc ggctggcata tcttagcatt
    62581 agcaactgct ggtattggtt atctatggct cagcccatac atttctgcaa caaaagcagc
    62641 attttatgaa caattgccaa aagatattta aaagagagtg tgacttgtga ccgagggggt
    62701 ggcaggagaa caccgtttat cgagaggcag taaaagaaac attcagccca ttagcaaaaa
    62761 ggaggtgtga caacattcat gtcacacctc ctttttgtat agttccagta aaaagaaaat
    62821 caacttagaa acagttaatc attcctcttc ttctgcttcc ggtaatcgat ggaacttgat
    62881 cagttcattc accgcgattg gtacgataga taaaatccct accaagaacc agtgattcaa
    62941 actgatgtgt actaggccga atagttcttg cgtaaatggc agcaaagcaa cagccgttgt
    63001 aatagctaat gccagtaacg ccatttctac caaagcttta ttggtcgcta aattgacttt
    63061 gaagatcgat tggctgctgc gtacattgaa cacatgcaga atcgatgaat aagctaagat
    63121 caagaatgca acagtttgac cgacatgctg acttgcgtct acccatgggc tgatagtatc
    63181 gacataacta cctaaataat aaccaaacaa ggttacgatg gtgaagaccc ctgcatttat
    63241 ggcaattttt tgcaataaac ctcgagcaaa gatcccttca tttttcggaa tgggtggctg
    63301 atccatgatg ccgtgttccg ctggctctct tcctaaagca aatcctggaa gtccatcagc
    63361 aactacattg acaaacagta gttgcacagc tgtaaatggt gctccccatc ctaacagcat
    63421 cgcaatcaaa acaataaaga tctccgaaat attacagctt agcaaaaagt tgatagcttt
    63481 acgaatattc tgataaaccg aacgtccttg actgacagcg tctacgatcg tcgcaaagtt
    63541 gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca tctgttcctg tgatccccat
    63601 ggcacaccca acgttacttg ctttcaacgc tggtgcatca ttcacaccat ccccagtcat
    63661 cgctacgact gcacccgtac gttgccaaga tttgacgatc cggatcttgt cttctggcgt
    63721 tacccgagca tagacagaga gagctttgac acgcgcatct aattcttcgt ctgataattg
    63781 atgcagttct gatcctgaaa gtgcttctga cggatcattc aagataccta attctttcgc
    63841 tatggcactg gcagtcacca catgatctcc agtgatcatg actgttttga tgccagcctt
    63901 tttcgctcgc ttgattgctc ctttactttc tggacgtggt ggatcgatca ttccgatcag
    63961 accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa atatcttgcg gttcttcgtc
    64021 ataagtagca taaccaacag caatcactct cagtgcccgt ttaccaaagc gatcattaac
    64081 gatcgccgct tgttctacat cgcctgaact aaagcgcgga gcaagaacat cgaatgctcc
    64141 tttggtgatt gagatgtatt tttcgcccat ttggtgaact gttgtcatca tttttcgttc
    64201 agaatcaaaa ggaagctcgt ttactcgtgg atatttttct tctaattctg cctttgtatg
    64261 ataattctct tcaacagccc ttacgatggc tacttctgtt ggattacctt tgataacaag
    64321 gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt gcagccattt tcaatatttc
    64381 catcgcgctg ttcgtcattg aatcttcgat attcgttact tcatcttctt tgctccatac
    64441 ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt ttgtctgagc agataacatt
    64501 agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg atcgcatgtt tcctcgccat
    64561 cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca gtcaaggttt ccggtacagc
    64621 tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg agtggttctc cttgcagttc
    64681 accaatcaca aatactaaag ccgcagccgc taaagcgatc atactgattc gtttgcctag
    64741 ctgattcaat cggacctgta aaggtgtttt ttgcatagta ttttcgttca aaagatccgc
    64801 gatcttaccc atttctgtag ccatgccaat ggcagtgacg atcgctttcc cacgacctgc
    64861 agcaaccgta catcctttaa ataccatatt aagttggtca cctaatgact catcttcttc
    64921 agcaataaat gctgcatctt tttcaactgc ttcactttct ccagtcaacg ccgattcttc
    64981 tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca gaaatagcat caccggactc
    65041 taaaacaaga atatcaccca ctaccagttg ctctgactca acagtggtca ctttgccgtc
    65101 tcgtatgaca gttgccgttt gtttattcat atcttgcaag gattccagtg ctttttctgc
    65161 gtttccttct tggacaatcg ccaataccga gttgatcaca acgatagcga tgatcagcag
    65221 tccttcaaat aaatccccat gttctgtagc aaatgcggta taaaaggaaa tcaccgctgc
    65281 tacaagaagg atgatcgttg taaaatcgga caagcttcga aagaattttt tgatcatcga
    65341 ttctttcggc gcctcatcga atttattcaa tccctgcttt tttcgccgtt catccacttc
    65401 ttgtgaagac aacccagtat ctggatctgc ttctgttacc ttctttactt cttctattgt
    65461 ctgcttgtat tcttccatcc tattcctcct ttaaaatctt cgtacaaaaa aaggtactta
    65521 ttttactata acatcctttt tttatttact tcaaataaag ggcttatcga ttatttgtta
    65581 agaaagcgta aaatttaaag tgcttatatt tgcaaacgct accaaaggat gctattatat
    65641 agatgtaagg aaggaggata ccatcaaata gacagaagaa tttctcgata gaacagtgta
    65701 agcaaacaaa gttgttagaa gtacaggttt cacccagaca tgcgtgtaga gtacaaacct
    65761 ataaatagtg aatgcgtatg gatgtttgga tcacgaagta tccgaaacaa gaagtaatgc
    65821 gatacgtaac aattgaataa cgtaaacgtc tgaaaattga ataggaacaa gagatgtttc
    65881 aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac atgaagaaat tcaacaccgt
    65941 gctttgcatg tatgatggaa agttacaatc atttctgttt atttgaatcc ttccgaggta
    66001 caagaaagcc gagcttatcg agagctcggc tttttctttg tcataaaatc tttgtaaagc
    66061 ttttttgtta ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc
    66121 aaaactaaag aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc
    66181 tcttcctgca aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt
    66241 tgagaaagct tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc
    66301 gctgtctgct ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga
    66361 agttgttccg acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc
    66421 cgcttcttcc gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac
    66481 gctgacaggt cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc
    66541 ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc
    66601 agcagaacca ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc
    66661 tttgatatat ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc
    66721 ttcatactca aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctactgcttg
    66781 caatctgcca tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggtttgat
    66841 caatgtctcc cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc
    66901 agtatctggt ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag
    66961 cgcatcacca ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc
    67021 agttttccga tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc
    67081 gattaattct tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac
    67141 tttctatcat gtgcttcatc attctatcat gtttttcgtt ctgttggcaa aatcagcact
    67201 ttttcacttc tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat
    67261 tgttttaggc atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc
    67321 atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac
    67381 gtcaatcttt catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga
    67441 aaaacgacga cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg
    67501 attgatggaa aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat
    67561 gttatccaaa gtattggatt gatgcctcac atgacgatcc gtgaaaatat cactcttgtc
    67621 caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat
    67681 cttgtggaat tgccccgtga gatgttagat cgctacccac atgaattatc tggtggtcag
    67741 cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac
    67801 gagccattcg gtgcgcttga tccaatcact cgtgattcct tgcaagacct tgtcaaagat
    67861 ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt
    67921 aaactagcaa gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca
    67981 gaaaatattt tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc
    68041 ctactacaag ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc
    68101 acgattacac cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta
    68161 gatacgttgt tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg
    68221 ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca ttttgaataa acatgttttt
    68281 tacgtaaaag aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcgggctg
    68341 aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca
    68401 ttagtggata tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca
    68461 gagtcagcta ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag
    68521 cacggttcag aaattctctc aaaaggtttt gaacatcttt atatttcagg acttgctttg
    68581 atcattggta tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct
    68641 gccacgattg tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg
    68701 gcattgatga tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt
    68761 tattcattat tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat
    68821 tatcgggatg tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa
    68881 ttgcccattg ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt
    68941 gcatgggcta cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt
    69001 ggactgaata attaccaacc agatctcatt ttggcaggta cgatccctgt aacaatttta
    69061 gcactattgg ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt
    69121 agagaggaga atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc
    69181 ttatacttgc gagttgttcc ttccctggtc ttgccagcaa tacagatgaa gacaccattt
    69241 cgatcacagg tggaatcact tcagaagcac agatcttagc aagtctagtt gccggaatgg
    69301 ttgaacatta tacagataaa aataccgcaa tcatcaacaa tctggctact acaactatca
    69361 accatcaagc gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag
    69421 atttaacgac tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg
    69481 tgaaagatga atttgagaaa agatacggtc aaacgtggtt cccgtcctat agttttgaaa
    69541 atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca
    69601 gcgatctgaa aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc
    69661 gtaaaggaga cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat
    69721 tacctatgca gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc
    69781 ttggctactc tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc
    69841 gtcaattctt cccaccatat gatgctgctc cagttgtaga caacaagata ttagctgata
    69901 cgcctcattt aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc
    69961 aggaattgaa ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt
    70021 tcttacaaga aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt
    70081 tcaacaattt ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg
    70141 tcacttcctg atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg
    70201 gatcatgatt tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat
    70261 ccagacgatc ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt
    70321 caatgtcgtg attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta
    70381 taccgggatg atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac
    70441 tgctagacaa cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg
    70501 cataagaaac gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc
    70561 tggtggtctt ggagatatca ttatccgagg aaccaatgca acagagggca catcgattat
    70621 tttagcaggt gcattgccaa ctgcattaat ggcgatcatc acggactggc ttttaggtat
    70681 tttggaaaga cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca
    70741 aaagcttctg cgaccatttg ttggtcgcaa aggctttttt agttctgtca tttttaaggc
    70801 aataatccac tctgattctt ccctccttat gggcaaagag ttgcttttct tagctattta
    70861 tccattccgt atactccaac ttcatactgc caacgggttc atatctgcat ccaccgacta
    70921 agtttttttc ttatccgcta aggcattttg ccaatccatt ttcccatcac tcgtcatgga
    70981 attcatacca catgatctct aaatactcgt ctgcttgttc tttgaattgg gcatcaattc
    71041 ttacatcctt tggcgtggct ttcatttaag ccttttccat cagtcctcga ttgttcgatg
    71101 ccttcagctg catcatcaaa actggtgact gccgtatcgg taacttcgat catcttttat
    71161 acaatcggga acttgccatc ctcatccttg gctaaaaaca tatctagcca ttccagcttc
    71221 tcgccatggg aggtttgcgg ttcctaaaac cggtacacga ttcttatgga ctgcatccgt
    71281 cgcaactaga cttggcgata cgatgatcct tcaccagaag agcacccccg taaaccaatt
    71341 gatcgatata ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg
    71401 cattgccgtt gatactgcta ttcatggccg aaagcgccac gactttaact tcttccgttt
    71461 gagtgacatt catgttcggc aatgtcgctt tggatacccg ctctgctaaa ggaacgacac
    71521 tggtattgta tttcgtatct gtatcttttc caaaagacca tgccaacgaa tcctctggga
    71581 accaataaga cgactccggt tgattgtcca ttgctttttt caacatactt ttggctgttt
    71641 ctcgataaac gacatttcca attgtcttcg cctcactttg gcgataatcg atggtcgttg
    71701 ccactgccaa cactaacaac gatgctccaa ttttactgat tttccttgtc acacttcttc
    71761 ctccttgttt tacataaacc cgtcaacatt ccattcctaa actcaaacaa tgacccttca
    71821 aaggtggaga tataggaaca aattaaagaa atcatgcaat aaatccaaga aggttggttt
    71881 tagaacaaaa aaagagtctc ctttttgggg agactctttg ttaactatct aaaaaggttg
    71941 gctcttctca cttcactcat aatgtgtcca ggcagaataa atttctacaa ctttattttc
    72001 ctcatcaact ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt atctgccttc
    72061 atgtgtaggt ctcagctttt caaaagactg cgtgggcata tatggatctt ccttcaatgt
    72121 ctgaatcact tcttcaaatt gtttctttaa attcgtttgc ttgatttttc ttaaatccac
    72181 tttcgcagag tttttgattg cgactgtata gttactcatc aaagagcttc ccaatcaata
    72241 tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt atctaaagta
    72301 cctgtttgct caagaaaaag ggtttcttga atcgctctcc agtcttccag cccaatcaat
    72361 acagcattgt ttccactacg atcactaata atctcgattt cagtatgatt ttcatttacc
    72421 tctttcaata actgataaaa atttttacga gcagtgctag gattcatcgt tgtattagcc
    72481 attgtctctc ctcctttttc tgatataatt atagcgtact tgattaagta cgtcaaaaaa
    72541 atacaataaa gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc
    72601 cctttttagg gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc
    72661 caaacgtaaa gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg
    72721 tgagtctatg caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat
    72781 atcttgaata acctgaggat acgcacgaaa gatttgtaac ggttattcaa aactgatttt
    72841 ggccgtcaag tcttctactg cctcaaaaat gattgcctct ttcgtctcct gtcattcatg
    72901 ttcactatcg atttgatact tgcttagacc gttgggtttg tgaatcacca attaaaaatg
    72961 gcactgagtt atggtaacaa aaaatggtca cttgacgagg ctgagattcc tacttatgaa
    73021 gacacatggc acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca
    73081 ttatttggca aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc
    73141 taaataggaa gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg
    73201 cacctttagc tgagaataag acaaaacaga ccctctagct tcaattaaga gctagagggt
    73261 ctgtttttga ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac
    73321 gatattttca cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat
    73381 attctgccag cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg
    73441 tttcgctgca tttttactta cgatatctac ataacggata attggatttt caatcgagcg
    73501 ataagaagag tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc
    73561 gatttctttt tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc
    73621 accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa
    73681 tacaagaacg gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg
    73741 ttctgctact tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat
    73801 aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg caatgattgc
    73861 atatgagatg aacgcacccg taatattggc aatcgatttg cttaaccatc tttttccttc
    73921 ttttttccat ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat
    73981 agagtataac ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga
    74041 acctagcgct agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata
    74101 catgtgaggc atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa
    74161 accggtgtta gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa
    74221 aataccatgt ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg
    74281 aacgatccca taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa
    74341 tgccatcatc gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac
    74401 agcattacta atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa
    74461 aatcaaagcg atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg
    74521 cacagcacct gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat
    74581 aaatgtgtac accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag
    74641 catcatcagt agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg
    74701 caccgctgat gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta
    74761 gtcgatcaat aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct
    74821 gacgacatat gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt
    74881 taatgaaatc aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc
    74941 agcagacaaa gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa
    75001 cgcatcagac gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc
    75061 agcagatttt aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc
    75121 tttacacaca attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat
    75181 agtacgctca ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga
    75241 aaaatatcaa gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg
    75301 gctttttctt tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat
    75361 cctttttgat atcttaactt ttggaagaga gatgtaaggc tgctttctac ccatcattct
    75421 tactactcga agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg
    75481 catctgcggt aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt
    75541 tcttattact tgatgcagag cagctttttt acaacctctt ggtacacggt gttcaatcag
    75601 ctgaccctcg gtattagctc aaaaaaacgg caaaatatga ggaactattt ttccattttt
    75661 ttctctaata ctcaggtcaa aacactgatt tcacaacctc tttttttaat gaaaaatatg
    75721 gctctcatgc gccgcttctt tgaatttcgt cattttttct tcgatgaacg gatagattgc
    75781 cgttcctacg attccaaaga caacaaacaa accgatcatg atccagattg ctttcgtagc
    75841 atttggccaa taaattcctc ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg
    75901 caagaacgga ttgatcattt ggaagaattt ccctgagact tggatcggat agttcccgcc
    75961 tccgccagag atcgacagta ctaagatgat aatagcgatc cctttaccga cattatcgaa
    76021 taatgcagcc aagacataga cgatcatcat gaacgctaag gcgatcaaga cagcaaatga
    76081 cacgctgtag accggatttt ggacatctac tcctagtaag aaaatattcc ctaatgtaac
    76141 gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc
    76201 tcgtttcgta aaattgtttc ggtctttctt tcctaaatag tattctgtcg tagcaacact
    76261 tgaaagaaga acagcgccga cccacagaca tagtgcagta taaaacgggg tgctagctga
    76321 accgttattt gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa
    76381 gtcactttct tttgttgcgt ctagtttcaa taatttgatg acttcgccta gatcgacttc
    76441 ttgttcgcct tttcggatcg cgtctgctgc tttttggata ccagctttga tatttggcca
    76501 gtcattgttg atcaagtccg cagcagcgtt cacagctgtt tcgacttctg gcattttttc
    76561 atttactgtt gtcaatgtac cggtgatttc attttttagt gctggccaat cattttggat
    76621 aaaatcagcg gccaatccta atttttgctg aatgatcggt aattcattgt tgtataggtc
    76681 tgccccttca ttgatgccat tgacgatggt atccatattt ccattcagca ttgtattggc
    76741 atcatggatt tcttgtccaa ttgcaggcat ttctttttga tatttttcta aaattgccac
    76801 tgcattcgtt acagttgctt tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc
    76861 tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg acttggtcta gtagagaagt
    76921 gacagtggaa ctgatttgac ctgtattgat accagcaatg atgccattga tattattaac
    76981 aacggcatca atttgatcta gcaattcatc aatagccttc gtgtcttcat tttgaacagc
    77041 tgtgttcaaa gcagataggc ggttttgcag attgcttagc aagggtttta attgatttag
    77101 ctgatcaata attggctgta atttcgtatt tccagcactt gtttgcaatt tagtcaggaa
    77161 ctcgaccagt ttatcgattg cattttgttg ttgagatatt ccgtcaataa agccgttcaa
    77221 tacattgctg atgtttttct tttcatcatc agtcagctta ccatcttcaa tagctgttct
    77281 tacagtatct attaacgaac ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac
    77341 atctacactg tttgtgatgc ttggtatcgc atctttcagc tgagaggcac ctgattttgt
    77401 ttggtttgct agatcacttg cctgattttc taaatttttc acatctggca agatatcttg
    77461 tacttgttgg atgatcgtca agccttgttt tgcttcatcg attccttggt tcattgtctc
    77521 ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat tttggatctc
    77581 tgggattttt tcttgcaaat caaggatgat ttttgcttga tctttcaact caggcatttt
    77641 gtcattcaat gcaacgactt tttcacccat ctcatctact ttagggatgt aatccacgaa
    77701 ttcatttgct ttattcagtt tttccttgat ttcaggtagt tgactttgta attcgaggac
    77761 ttgttttgtg tagccgtcga tcgtgtccaa attttcatct gttgataaaa tcatatcttt
    77821 aa
//
LOCUS       DACEDM010000004        61314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEDM010000004 DACEDM010000000
VERSION     DACEDM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.004"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            142..1602
                     /locus_tag="IWO50_000251"
     CDS             142..1602
                     /locus_tag="IWO50_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357405.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1823..3586
                     /locus_tag="IWO50_000252"
     CDS             1823..3586
                     /locus_tag="IWO50_000252"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0357406.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3670..4128
                     /locus_tag="IWO50_000253"
     CDS             3670..4128
                     /locus_tag="IWO50_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0357407.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     NKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            4237..4920
                     /locus_tag="IWO50_000254"
     CDS             4237..4920
                     /locus_tag="IWO50_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0357408.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            5067..5681
                     /locus_tag="IWO50_000255"
     CDS             5067..5681
                     /locus_tag="IWO50_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0357409.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5697..5903
                     /locus_tag="IWO50_000256"
     CDS             5697..5903
                     /locus_tag="IWO50_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357410.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            6444..7574
                     /locus_tag="IWO50_000257"
     CDS             6444..7574
                     /locus_tag="IWO50_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0357411.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            7666..8421
                     /locus_tag="IWO50_000258"
     CDS             7666..8421
                     /locus_tag="IWO50_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0357412.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            8479..10518
                     /locus_tag="IWO50_000259"
     CDS             8479..10518
                     /locus_tag="IWO50_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0357413.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(10627..11778)
                     /locus_tag="IWO50_000260"
     CDS             complement(10627..11778)
                     /locus_tag="IWO50_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0357414.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(11932..12462)
                     /locus_tag="IWO50_000261"
     CDS             complement(11932..12462)
                     /locus_tag="IWO50_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357415.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(12561..12911)
                     /locus_tag="IWO50_000262"
     CDS             complement(12561..12911)
                     /locus_tag="IWO50_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0357416.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(13068..14597)
                     /gene="ahpF"
                     /locus_tag="IWO50_000263"
     CDS             complement(13068..14597)
                     /gene="ahpF"
                     /locus_tag="IWO50_000263"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0357417.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(14607..15170)
                     /gene="ahpC"
                     /locus_tag="IWO50_000264"
     CDS             complement(14607..15170)
                     /gene="ahpC"
                     /locus_tag="IWO50_000264"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0357418.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(15314..15976)
                     /locus_tag="IWO50_000265"
     CDS             complement(15314..15976)
                     /locus_tag="IWO50_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0357419.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            16160..17806
                     /locus_tag="IWO50_000266"
     CDS             16160..17806
                     /locus_tag="IWO50_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0357420.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            17797..18879
                     /gene="uxuA"
                     /locus_tag="IWO50_000267"
     CDS             17797..18879
                     /gene="uxuA"
                     /locus_tag="IWO50_000267"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0357421.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(18987..20540)
                     /locus_tag="IWO50_000268"
                     /pseudo
     CDS             complement(18987..20540)
                     /locus_tag="IWO50_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            20688..21829
                     /locus_tag="IWO50_000269"
                     /pseudo
     CDS             20688..21829
                     /locus_tag="IWO50_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(21955..23034)
                     /locus_tag="IWO50_000270"
     CDS             complement(21955..23034)
                     /locus_tag="IWO50_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0357422.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(23137..23577)
                     /locus_tag="IWO50_000271"
     CDS             complement(23137..23577)
                     /locus_tag="IWO50_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0357423.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(23634..24044)
                     /locus_tag="IWO50_000272"
     CDS             complement(23634..24044)
                     /locus_tag="IWO50_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0357424.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(24224..24658)
                     /locus_tag="IWO50_000273"
     CDS             complement(24224..24658)
                     /locus_tag="IWO50_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0357425.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(24758..25606)
                     /locus_tag="IWO50_000274"
     CDS             complement(24758..25606)
                     /locus_tag="IWO50_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0357426.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            25995..27761
                     /locus_tag="IWO50_000275"
     CDS             25995..27761
                     /locus_tag="IWO50_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0357427.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            27863..29122
                     /locus_tag="IWO50_000276"
     CDS             27863..29122
                     /locus_tag="IWO50_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0357428.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            29269..30570
                     /locus_tag="IWO50_000277"
     CDS             29269..30570
                     /locus_tag="IWO50_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0357429.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            30573..31424
                     /locus_tag="IWO50_000278"
     CDS             30573..31424
                     /locus_tag="IWO50_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0357430.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            31437..32252
                     /locus_tag="IWO50_000279"
     CDS             31437..32252
                     /locus_tag="IWO50_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0357431.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            32403..33569
                     /locus_tag="IWO50_000280"
     CDS             32403..33569
                     /locus_tag="IWO50_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0357432.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            33799..34446
                     /locus_tag="IWO50_000281"
     CDS             33799..34446
                     /locus_tag="IWO50_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0357433.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            34530..35474
                     /locus_tag="IWO50_000282"
     CDS             34530..35474
                     /locus_tag="IWO50_000282"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0357434.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            35609..37060
                     /locus_tag="IWO50_000283"
     CDS             35609..37060
                     /locus_tag="IWO50_000283"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0357435.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            37081..37917
                     /locus_tag="IWO50_000284"
     CDS             37081..37917
                     /locus_tag="IWO50_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0357436.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            37917..38576
                     /locus_tag="IWO50_000285"
     CDS             37917..38576
                     /locus_tag="IWO50_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0357437.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            38702..39391
                     /locus_tag="IWO50_000286"
     CDS             38702..39391
                     /locus_tag="IWO50_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357438.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            39397..40353
                     /locus_tag="IWO50_000287"
     CDS             39397..40353
                     /locus_tag="IWO50_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0357439.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            40397..40909
                     /locus_tag="IWO50_000288"
     CDS             40397..40909
                     /locus_tag="IWO50_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0357440.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(41165..41713)
                     /locus_tag="IWO50_000289"
     CDS             complement(41165..41713)
                     /locus_tag="IWO50_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0357441.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(41802..42983)
                     /locus_tag="IWO50_000290"
     CDS             complement(41802..42983)
                     /locus_tag="IWO50_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0357442.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(43219..44727)
                     /locus_tag="IWO50_000291"
     CDS             complement(43219..44727)
                     /locus_tag="IWO50_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0357443.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            44886..45077
                     /locus_tag="IWO50_000292"
     CDS             44886..45077
                     /locus_tag="IWO50_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357444.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            45280..46197
                     /locus_tag="IWO50_000293"
     CDS             45280..46197
                     /locus_tag="IWO50_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0357445.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            46190..47263
                     /locus_tag="IWO50_000294"
     CDS             46190..47263
                     /locus_tag="IWO50_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357446.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            47411..47929
                     /locus_tag="IWO50_000295"
     CDS             47411..47929
                     /locus_tag="IWO50_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357447.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            47910..48302
                     /locus_tag="IWO50_000296"
     CDS             47910..48302
                     /locus_tag="IWO50_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0357448.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            48378..49313
                     /locus_tag="IWO50_000297"
     CDS             48378..49313
                     /locus_tag="IWO50_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0357449.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            49446..50225
                     /locus_tag="IWO50_000298"
     CDS             49446..50225
                     /locus_tag="IWO50_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0357450.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            50218..50535
                     /locus_tag="IWO50_000299"
     CDS             50218..50535
                     /locus_tag="IWO50_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0357451.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            50779..52389
                     /locus_tag="IWO50_000300"
     CDS             50779..52389
                     /locus_tag="IWO50_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0357452.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            52546..53394
                     /locus_tag="IWO50_000301"
     CDS             52546..53394
                     /locus_tag="IWO50_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0357453.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            53625..54104
                     /locus_tag="IWO50_000302"
     CDS             53625..54104
                     /locus_tag="IWO50_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357454.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            54137..56365
                     /locus_tag="IWO50_000303"
     CDS             54137..56365
                     /locus_tag="IWO50_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0357455.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            56494..58137
                     /locus_tag="IWO50_000304"
     CDS             56494..58137
                     /locus_tag="IWO50_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0357456.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(58210..58755)
                     /locus_tag="IWO50_000305"
     CDS             complement(58210..58755)
                     /locus_tag="IWO50_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0357457.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(58778..59263)
                     /locus_tag="IWO50_000306"
     CDS             complement(58778..59263)
                     /locus_tag="IWO50_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0357458.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(59253..60065)
                     /locus_tag="IWO50_000307"
     CDS             complement(59253..60065)
                     /locus_tag="IWO50_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0357459.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(60204..>61314)
                     /locus_tag="IWO50_000308"
     CDS             complement(60204..>61314)
                     /locus_tag="IWO50_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0357460.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        20471 a        10953 c        11915 g        17975 t
ORIGIN      
        1 tgtgagcttg tttgactttt gtcccagttt ctttttttat tggtttttga aagggctttt
       61 ctaaataaaa aggaaacaaa aatacgattt acagatatga ttaaataaaa tcataagaaa
      121 aactggaggc gtgtatcagg aatgctggaa aattacatcg agcgaaatat atttagaaaa
      181 gtgtatctgt gtgaacaatt atttgaattc caagaaatag acatcgaaca aacagctatt
      241 tctctaaggg tgactacacc aacgatcctt cacgatctag aaagtctagc cgagtgtcta
      301 gaatattgta taaaagaaca agtaagagag aagcataagt ataaactcgt attcaaacat
      361 ggtattgctc tttctgagct cacacagttt ctgtacggtc aatcttattt tttaaagttt
      421 ttaagttatt attttaatgg tatattcacg agtacagaac tggctgatct tgaatttatt
      481 tctttgagta aagtatatac aatcaaaaaa atcgtattgg atttttttaa agaaaatgat
      541 tatttgaaaa ataaagaaat cattatccca gaatttgact cgcggaacat cttgctagct
      601 ttagtacgtt atgtcaattg ggaaggctat gaaaataaaa atccgtatgt agaagaagct
      661 tgtaacgagt tgattgatta tgtagagact cattttttta aaagaagata ttcggaagat
      721 gaaaagtcct ttatcatacg agaaatagag atcgcaatcg gaagaaaggc atttcccctt
      781 ggactgaaag aagaagataa gaaaagcgca gaaacgaagc cgttatttca attagtctgt
      841 caaggattgg aagagcaaaa aggaattcta gacttacaag aagaagatgt ctattatatt
      901 ttttctttgt tcaacagtag aaattatacc aatgagaaca tggagctgtt aagaaaagat
      961 ttcgaagttg tttataaaaa ttttgttctc aatgacctat cactgaatga attgatttta
     1021 cagattatta gtatcgctga aatcgacgat gcagaaaatt ttattttcaa gcaatccttt
     1081 ttacaatttg tccgtacgtt gtgggcagat ggacaagtat ttttaccgga aaggatctat
     1141 cttctatcag aaaaagaaca acttctgtac ggaaaaattg tagaaatatt ggaagagtgg
     1201 aaaaagaaaa atcaaatcca actaagatgg aatgaaaatt tcattcgaaa attcgtcaaa
     1261 agtctttctt tgatcaacag tgaacaagcg gggagacagg aaatagaaat ttttgttgtt
     1321 tcagaaagtt cagtcaaaca atttttttat cgaacccaat ttcatttgta tgcggaagaa
     1381 ggcatcgctg aaataaatcc tattttattt cgttcattag aagaattgcc tgatgaatgt
     1441 ctatgtgcga aaaaaagagt ggttttgtgc gatgctgcta gctatcaaaa tggattagaa
     1501 actgaaaaga caaagatcta tccaatctca ttaaaaggtg caaacatcca tttgtatcat
     1561 attgctcagg aacttcacct attaaaaaaa catatttctt gatgagacct taattaggag
     1621 accttctaca aagcttttta attgtctgta gaaggttttt atttgagtcc tgattcgtgc
     1681 gaagatctac ttatataaca agctttctct gaaatttttt taaaatgcta ttgacttaga
     1741 gtcaactcta agtagtagaa tagataaagt gtaacaaatc tattttcatt aaaagaagat
     1801 agtacggagg agagaatgta ttatgagtac agtattacat gtattggatg caatcaatag
     1861 ccccgcagat gtcaaaaaat tatctgttga ggaaatgaaa cagttggcag aagagatgcg
     1921 ccaaaaaatc ttgcaaaagg acagcgatgt aggtgggcac gttggtccga atctaggtgt
     1981 agtagaatta acgattgcgt ttcattatgt atttgattca ccaaaggata aaatcgtttg
     2041 ggatgtttcc catcagtctt atggtcataa gcttttaact ggtcgaaaaa atggttttga
     2101 aaaaggacat taccgtgaag tgagcggata ttccaaccaa catgaaagta aacatgacta
     2161 ttttacagtg gggcacacat ctacatctat cagcttagca acaggtttag ctaaagcgag
     2221 agatctgaag aaagaatcag gaaatgtggt agcatttatc ggtgacggct ccctaagtgg
     2281 tggcttagct tttgaaggat tgaacaatgg tggtgtttta caatccaatc taatcgtatt
     2341 agtaaacgat aatgagatgt ctattgatga gaatcatggc agtttatata aaaacttagc
     2401 tgagcttcgt caaaccaacg gtaaaagcag caataaccta tttaaagcaa tgggatttga
     2461 ttatcgttac gtggataatg gaaacgatct ggaaacggtg atccgtttat ttgaagaagt
     2521 aaaagatatt gatcatccga ttgtacttca cgtccatacg gaaaaaggtc gcggttatca
     2581 gccggcaatc gataataaaa tgaaatatca ttggcatgta ccatttgatc tgtccaccgg
     2641 ggaggcaaag caagtaactt ctggagaaac atttaatatg atcatcatgg attatttgga
     2701 taagaaaatg caaacaagta cagctcctat tgccgcagtc aatgcagcga taccaggcgc
     2761 ctttggactg aaaacattcc aagaaaaaca tccagatcat tattttgacg tggggatcgc
     2821 tgaacagcac tctatcactt tcgcttctgc tttggcttct cagggagtac gtccagtcgt
     2881 cttccaatca agtactttct tgcaacgggc atatgatcaa ttatcacatg atttagcgat
     2941 caacgagaat ccagtgatta ttttagtcgg tggcggaacg atctcagcta gtgaccgaac
     3001 acaccaagga acatttgaca ttccttactt atcaagtatc ccgaacatca aatatcttgc
     3061 tccagcaaca aaagaggata tgatctctat gctagattgg gcgttcgaac aaacagcgca
     3121 gcctgtagca atccgaattc ctacacatgg tgtagaacat ggtacagcta tcgcaaatga
     3181 ttatagtatt ccagcctatc aaacagtaaa taatggagga aaagtagcaa ttttagcttt
     3241 aggcggtttt cttgaattag gaaaagaaac agtaaaagct tttagaaaaa aaggaatcga
     3301 agcaacgttg atcaatccag gaacgatcac agaaaatgac gtagaaacat tagataaatt
     3361 aaaagaaaca cacgaattag tcatcacatt agaagatggt agtttgtcag gaggatttgg
     3421 cgaaaatatc agtcgttatt atggtgattc ttcaatgaac gtattaaatt ttggtgcaga
     3481 gaaagaattc acggatagcg ttcctgtagg gcagctttat gaacgttatc atttaacacc
     3541 aaaacaaatc gttgaagata gtctgcgtgt cttgaatatt caataattca ttatgctcac
     3601 catccagata attgtttata ctagtatctg gatggttttt tcattataaa aagcaaaggg
     3661 gtggatgtta tgtatcagca agtagaagaa tatgtcaaaa agttccaaga aaaatatcct
     3721 agttatcaat cagaaaaatt aggtttcttt tcatttgggg atacagaaaa aatggcagac
     3781 gagttgagcg aactagttgt aaataagata aagactggta catcaagtgg ttacgatctg
     3841 tataaacaag agaatgagcc tttaccaaaa gttggtcaat tagacgtggt cctgaatgga
     3901 aaaaatgaac cagtttgtat catcaaaaat ataaatgtga ccatcttgcc ttttagtgaa
     3961 gtcacgaaag agcatgcttg gaaagaagga gaaggggatc ggacattagc ttattggcgg
     4021 aaagtccatg ctgagttttt taaacatgct tataaagaag cgttgaacat agaatttaca
     4081 gaagagaagc tggtcgtata cgaagaattt gaagtaattt tcttttagta agaaaggtct
     4141 gctgaaagaa atagctcatt attgtctcct gaatagaaaa gggttacatt tttatatgaa
     4201 gctgtatttt tatataacat tactggagga gatattgtgg atacactgga acaaatcaaa
     4261 ggcggagtcg tcgtttcttg tcaagctttg gaaaacgagc cgctacacag ttcttttatt
     4321 atggggagaa tggctttggc tgcaaaagaa ggcggagcag ttggtattcg agcaaatacg
     4381 acagaagata tcaatgaaat caagcgaatc gttgatttac cggtaatagg gattatcaaa
     4441 agagattacg atgggtcaga agtgtttatt actgcgacag aaaaagaagt ggaagaactt
     4501 ttggagacgc aagcagaaat cattgcactg gatgcaactt ctcgtaaacg tccgacagaa
     4561 aaaacaactg ctgaacttgt ccaaatgatt cacgaacacg gtcggttagc tatggcagat
     4621 atttcgactt ttgaggaagc gcttcaagcg caagaagatg gttttgatct tttgtctacg
     4681 actttagcag gctatacaga atattcacga aagacgcaga cacctgattt tgatttattg
     4741 aatcaaatca tagaacaagt cgatatccca gtaattatgg aaggacatac tgactctcct
     4801 gaacaagtag agaaagcata tgaattaggt gctttttcag tcgtagttgg atctattatc
     4861 acccgtccgc aattgatcac taaaagatat gtcgaagccg caagtaaagc aaataactaa
     4921 aaaacacgca cggcacagat tcttgacaaa aaagaaaaat tgatagatag tttaaagcag
     4981 attctaagaa gatgaaaaaa gcattacttt tcattttgtc ttcaagatct gctttttaac
     5041 gaattttata gaaagaaggc gaaaccatgt ttgaacggag tccttcagaa ttgacagaac
     5101 gtgaaaatta caagttgctg attggttcag tcatccctag accagtagca gtagtaacga
     5161 ctaaatcagc aaaagatata gtgaatatcg cacccttcag ttacttcaat atcgtcagtt
     5221 cgaatcgacc catcttatcc ttggcgatcc agagaaaaca agatgcagta aaagacacag
     5281 caaagaatat tcttgagaca agagaagctg ttatccatat ccttgatgag gacaacgtag
     5341 aagaagtaaa taaaacagca gccaatctgc ctaatgagga aagcgaactg tacctgactg
     5401 acttgacttt aactgattca gtaatcattt cagtactagc attgacagaa gcaaaagttc
     5461 gttttgaaac atccttatat gaacatgtcg agatacggaa taaaaataaa gtaacagctg
     5521 atttattttt gctagaagta cagaaatatc atttaaatga aacagtctat gatgataaga
     5581 caggaaggat cgatccaaga aaattgaatg ctgtaagccg tttggcagga aatgattatg
     5641 cagcaatagg agaaattttc acgattgcac gtcccgaata aaaaggagca ctagttatgt
     5701 taaaaataag ctataaacca tcgacagatt caaaagaaat gaaaaaagaa tatgaaacag
     5761 tgaatgattt tctgcaaggg cagtacttag aagttccgcc attgcaagac cattttgtcg
     5821 ttactactgt tactttagat ggtaaggaaa tagaaatgcc agatcaaacg attagcggat
     5881 tgtttaatta ctttaacaaa taaatattta ttccgaaaag tgaaaaaaca tataaaaaaa
     5941 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
     6001 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
     6061 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
     6121 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
     6181 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
     6241 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
     6301 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
     6361 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
     6421 gaaaaaagat cgattggtgg attttgggtc cttatttgac tttgtccatg atcggtcttt
     6481 tagaagtata cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat acaaaaagtc
     6541 tattattgcg tcaactaatt ttcatatttt tgagttggag cgtgatcttc ttagctcgtt
     6601 cagtcaaact acactattta cttcacccta aaatagcagg atacggttta gccttatcga
     6661 ttttcttttt agtattagta agaataggga tattcggtgt cactgtcaac ggcgcacaac
     6721 gttggatctc tctgtttggc attcaattcc agccttctga actggcaaat ctttttttga
     6781 ttttttattt aagctggttt tttcgtgacg gaaatagtag cccaaaagat ctaaaaaaac
     6841 cattcctgat tacagtaggt ataacttttc tgattttatt tcagccaaag attgctggag
     6901 cattgatgat cctttcgatt gcgtgggtca tattttgggc agcggcggtt ccatttaaaa
     6961 aagggatcta tctaatcgtt actttttctg cattgctgat tggagcagca ggcggggtat
     7021 tatatttagg aaataaaggt tggcttccac agatgtttaa tcatgcttat gaaaggatcg
     7081 cgactttaag agattctttt atcgatagtc atggtgcggg ctatcaaatg acccattcgt
     7141 tttatgcact atataatggt ggcatatttg ggcggggatt aggaaatagt atcacaaaaa
     7201 aagggtatct tccagagaca gaaacagatt ttattttttc gattattaca gaagagcttg
     7261 ggctaatcgg tgcattgtgt gtgcttttct tattgttctc attatgcatg agaatctttt
     7321 gtttaagcag tcgctgtaaa aatcagcaag ctggattgtt tcttttagga ttcggaactt
     7381 tgctattcgt tcagacgata atgaacgtgg gaagtatcgc ggggctgatg ccaatgactg
     7441 gtgtgccatt gccatttgtt agctacggag gaacaagtta tctgatcctt tctttaggaa
     7501 tcggaatcac gttgaatatt tcttcaaaga tacaggccga agagttggcg ctctatcgac
     7561 ctgaaaaaca ataaagagaa actgagaaac tctcagtttg aattatcata aagtacaatc
     7621 ttttattttt caagcgagag gttcttttag aaagaggaat tcgcaatgaa agtatctcaa
     7681 aaaatcatat tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag
     7741 tttatccatg gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa
     7801 agtatcccta ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa
     7861 aacggtcgct cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa
     7921 ctgatctcta tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata
     7981 aatgcagctt actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc
     8041 aaatttgaag ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat
     8101 gaactgtttc ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta
     8161 tatataaaca aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt
     8221 cgggaagaca aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct
     8281 atctctcagc agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa
     8341 ttacttggta gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat
     8401 ttcctcaaag acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag
     8461 caagagaagg aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct
     8521 tatattgccg gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag
     8581 cactatcaag aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa
     8641 gctttgaaca agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat
     8701 aaaagtgcat tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct
     8761 gatgtcaaag gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac
     8821 agtttttcat ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat
     8881 aaaggaacat tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt
     8941 tttccagaaa tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg
     9001 aatattatag atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga
     9061 gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct
     9121 tcctccttcg acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa
     9181 cccgattact ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga
     9241 gctaccattc aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg
     9301 attggttacg tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt
     9361 aatggaaaaa tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca
     9421 acaggtggga aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa
     9481 catgaagttc aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca
     9541 gcttttgaca gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt
     9601 gatcttcttg cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc
     9661 tcacaagaag attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt
     9721 gcgacaggtt atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac
     9781 aacggcacta tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac
     9841 agttcttggg gatcttatca agcaacgcgt gtcagtagtg atgtatcaca agtagactta
     9901 aaaactgctt tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt
     9961 gagaaaaaat ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca
    10021 attagtatga atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta
    10081 gctgatactg gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg
    10141 tattctgttt ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag
    10201 acaaaagata agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg
    10261 agagaagttg tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg
    10321 ttggcagcga aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag
    10381 aacagtttct tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg
    10441 gaaaataaag aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc
    10501 aaccaaaatt atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc
    10561 gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg
    10621 atttactcac acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag
    10681 tgtacttaat aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact
    10741 ttgctgtcca atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc
    10801 gaccatcaat gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag
    10861 caatttggtc aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa
    10921 gtcagaaaaa tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata
    10981 tcctaaagcc tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat
    11041 cccagcaaaa aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat
    11101 tcctaaacaa ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc
    11161 aaatgaaagt aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat
    11221 tagctgttca atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa
    11281 agataggctg aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa
    11341 aattgttgat ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat
    11401 actgacagaa gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg
    11461 gaatgctttg cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac
    11521 gaacatacca ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa
    11581 aaacattaat aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac
    11641 ccaattcaat ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg
    11701 gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa
    11761 aataccaata aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat
    11821 aacatacatt attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga
    11881 tttttttgga ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat
    11941 agtcattttt cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat
    12001 aaagccgaat atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa
    12061 tccgccaatc aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat
    12121 gctgaagaac aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag
    12181 gatagaaacg attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag
    12241 accactgaac agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa
    12301 aagattcaac aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa
    12361 aacagcagaa gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa
    12421 taaggcgtat ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg
    12481 acttatttta acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga
    12541 gaagaatatc tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct
    12601 agctcatcga tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct
    12661 tctactcgta caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg
    12721 gcttccgcaa aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt
    12781 tgggaataac ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt
    12841 ctctccgctt ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga
    12901 ttttgcgcca taatcagtca cctttctttt tttcaaatta tttactactt atagtatata
    12961 agaaaaagag aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt
    13021 gtcaccatcc ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc
    13081 agataatcga atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt
    13141 ttgtatggac tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca
    13201 tttttctcgg tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc
    13261 gtattaggga gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc
    13321 atcgtacggc gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc
    13381 gtcgctgcat gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt
    13441 tgatctgctt ttaattctgg taagaattct agaacatata catgttttgc taacccagcc
    13501 aaatcgattg ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct
    13561 ttaaataaag ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt
    13621 tcgcctggta cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct
    13681 ttaagtgata taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa
    13741 acggcctgtt gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc
    13801 tgcatcaatt ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt
    13861 tcaacgactt gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct
    13921 gcatagatag ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt
    13981 tctttttcaa acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg
    14041 atcgtcatgc gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg
    14101 atttttttat catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata
    14161 tttggattta atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa
    14221 gtcaagctaa cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc
    14281 aaagattcac tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag
    14341 gaaaattcat gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga
    14401 ttgatagaaa aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac
    14461 atttctgcta tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa
    14521 ctaacagaaa aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta
    14581 gtttctttgt ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc
    14641 aatgtttcgc cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca
    14701 tattgagcag cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca
    14761 ttgatttcca tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct
    14821 aatccttgct cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg
    14881 atcatcacgt attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta
    14941 aagtgggtat cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat
    15001 tgttcttgta agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag
    15061 caaagaatac tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga
    15121 tgataagcat gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct
    15181 ccaataataa ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga
    15241 tattttgtta tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg
    15301 tcgcaagaaa atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt
    15361 ctttcagcat catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat
    15421 caactaaagc cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct
    15481 gaatggtcac tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt
    15541 tccaaacttc ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac
    15601 ggaaaaaagc gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt
    15661 ctagcactat tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt
    15721 ccaactgttc tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat
    15781 acgtaccact ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt
    15841 cgcgaatggg agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc
    15901 ttccaggttc taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt
    15961 gcaagctgct tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa
    16021 gaggtctgaa aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa
    16081 ttttggttga catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg
    16141 ttagtttagg aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt
    16201 atctcaaaaa gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca
    16261 aaaaaatcct gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga
    16321 aatcgctcaa cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac
    16381 attcgatgaa gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt
    16441 ggtcatgcca agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct
    16501 gtattgtcat ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc
    16561 tagtttacaa ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa
    16621 taataaatat ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca
    16681 tacaatgagt attcttgctg tgctacttta taaacgtttt ttatctggag cttggccgat
    16741 tgcacttgtc tctacagata atttttcaca aaacggtagt caattacgta cttctttgct
    16801 aacgatcgca aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt
    16861 atcgaatgaa gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc
    16921 ttccttgtca gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca
    16981 aaccgcaaaa ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat
    17041 catggaagat cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac
    17101 tgatcgtgca acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt
    17161 gcatacgtct ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat
    17221 gaaagatcca gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt
    17281 tgtggagtct ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg
    17341 cttttctaat ccaaatattc ctaatatgcc tcagcgaatc gccacggata cctctcaaaa
    17401 ggtggcgatt cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc
    17461 gcagacactt gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat
    17521 tgatgatcaa ggaaaaccat tcatcccgag tccagaccca ctattgacag accttcagca
    17581 taagctttcc gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc
    17641 tcctattttg tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag
    17701 aaaaatagaa gaaatgttca tcaagatggt agcaggacca aatgccgtac gaaaaacatt
    17761 acaggattac ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac
    17821 ggatcacagg aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg
    17881 attgtaggca cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa
    17941 gaactaaaag aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat
    18001 gtgcatgatg ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag
    18061 cagacaatcc gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct
    18121 gtatttgact gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta
    18181 gcttttatat ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat
    18241 tccagcaatg aatttggatt gccgggatgg gaacctgagc gcttagctcc tatcaaatcc
    18301 ttgcttgaag cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt
    18361 ctccaatcaa tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat
    18421 gatccgccat actcgatttt tggattagct cgaatcgtaa agaatcgaga agatttagat
    18481 tggttgtgtc gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt
    18541 gcagaagatc cagaaaatga tgtatatggg attttagcag aattttgcca aagagatcga
    18601 atcccatttg cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca
    18661 ccacataaat ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac
    18721 aacggattta acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga
    18781 agaccaggat acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg
    18841 gaagcaatcg aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat
    18901 tatgtaaata agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc
    18961 attcggattt atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga
    19021 tccatgagtg cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt
    19081 ttccagtctt ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc
    19141 aattgtttca aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt
    19201 attttgatcg attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata
    19261 taaaaatcac gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca
    19321 tacaaatgta ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt
    19381 aacaagtgtt gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact
    19441 tctttcaata cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga
    19501 ttgaagttaa gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc
    19561 ttttcttttt caggtataaa caaatcaaat acagtacaaa gggactccac catttctctt
    19621 gaataaagaa catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt
    19681 tcttccgatt cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt
    19741 tcgcgacaaa ttggcagatt aggtcgcaaa atatctacag agagatcaat aaaaaagtca
    19801 atatattcat actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc
    19861 tctttacata tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca
    19921 agtacttctt ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca
    19981 taaaattctg tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga
    20041 atttgtacct cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa
    20101 ccgtactatg ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta
    20161 tagtttgttt gctgactgaa actgataatc gaatttgtct gacagctgaa caaagaagtc
    20221 aagatctgat aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa
    20281 gccggatcaa ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt
    20341 tctgttagat tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt
    20401 gattcagaca tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca
    20461 gataggaaaa gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta
    20521 atcgcttctt gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa
    20581 atattactgt ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc
    20641 tactatctta tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa
    20701 ccatcttaat atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc
    20761 acacagagga gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa
    20821 ttacctttca ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac
    20881 aaacagcgac catcaaagca aaaattgact attcgggaga cttagcacct atactacaat
    20941 cagctgttac atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt
    21001 tgactatcca acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt
    21061 taaaaccagc gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa
    21121 cgccgtatag tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc
    21181 ttgttgaaga accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag
    21241 aaaatccaga cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa
    21301 ctcaaccaga gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg
    21361 taactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
    21421 cagataatga aactgaaaat ccagaaaaac cagaggtaga tccagaagaa aaaccagatg
    21481 tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa
    21541 aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa
    21601 taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa
    21661 ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa acgaaaaata aacagctacc
    21721 aaaatccgga gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat
    21781 agcaacaatg ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa
    21841 tgtctgtgaa cgcatttttc aaataaaaag aaatatccag aaaatgaccc caaaatacca
    21901 gattgaaatc tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg
    21961 gatatctgtt tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa
    22021 gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac
    22081 aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg
    22141 attcagcaca taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac
    22201 atctcggatg cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac
    22261 acctgctact gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg
    22321 aaagccaaat acagccaagg cgattgtagt gagcaacgta tttaaaagag cgacaacaaa
    22381 ttgagcttct aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc
    22441 ttgaaagaac caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt
    22501 cttttcaatc ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt
    22561 tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa
    22621 atcgtttcgc tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg
    22681 atgctcgtaa aaattaataa cggactggat catgtcaaat gtttgatgaa taagcatcgg
    22741 gatatacttt gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc
    22801 taaaacacct gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa
    22861 ataagtaaag ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac
    22921 aaaaacgaca aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg
    22981 ataaacggtc aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg
    23041 atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga
    23101 gttggttttc ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg
    23161 aaaattatct agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag
    23221 tggaagataa gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc
    23281 ttgagcagtc agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat
    23341 ataatttttt tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa
    23401 atcatttcct agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag
    23461 atactgggta taggtaagat tcagcggctt gaggagggga gtgtagtgtc taatgatttc
    23521 tttagaggcg gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt
    23581 tccacctccg ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca
    23641 ctaactgctt tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact
    23701 ttatgcgctg cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct
    23761 tcaatctcta cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca
    23821 ctgacagtag aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta
    23881 aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt
    23941 ttaccaggag ctgcaatatt caattcgaat gtacgatcgg gattatgcac ttctccagag
    24001 cgtccgcctg tgttgatcat tttcgtagaa tacatttttt tcataaaatt accaccttta
    24061 attatatttt atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt
    24121 ggcataagat aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat
    24181 atacaaaaaa tatcagagac aaaaaataat ctcatcataa acattacata tatttctcaa
    24241 aaagttgttt tctttcttca aaaattactt cacgatggac ctgatgaagc atccataaat
    24301 aaccaaaagg atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt
    24361 cttgaatctc tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga
    24421 cgatgatatt gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag
    24481 acgtgttttc atccagcata tggaatctcg taccatacaa ggtaaaaatg acttcattat
    24541 tgcctctagg gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca
    24601 gttctagtgc tttcaaacta tcatttaata caaaatcgat ttccgttcct ttatgcataa
    24661 agatctcctc tccttttcag tcgaacagtt actagattct tttattatac cagctaaaga
    24721 aaacgcattc aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa
    24781 atccgcgtcc cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt
    24841 cttctatcaa tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct
    24901 cttttgactt tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct
    24961 cagcacttaa gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt
    25021 tctttcgatt gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata
    25081 aaatagcata aagaaactct tctattccaa aaataaacag attaagagaa acaacgaatg
    25141 cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg
    25201 gagggatcgt cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag
    25261 caaaaatcgc acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg
    25321 gaactattgc aatgattaaa ggaaaaacca agcttccgaa aagtacttta aaaaagggtt
    25381 ttttacctaa aaatagaaga gctagtacca acataacgat attcaacact aaaataacta
    25441 tagctcggtc aaagcctaaa gcagcttcca gtaatattcc tagaccgctg acacctccag
    25501 cagcaatatg atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa
    25561 aaataatctt aatgtatgtc catataaatt gtctcgtttc tttcatttat tcacttcctt
    25621 aattcattaa taatactctt atcttattat aacttacttt tcaccttaag ttaactttaa
    25681 ttaataaaaa aattaggcta gtctaaatta ttatgtttca ttcttctaga atatacttct
    25741 acgccaaaaa ctgttaaaaa acttttactt tagaaaattg gagaaattga tcgcttaaga
    25801 taaattcagg acactaaata gtacaggaag tagactaaaa cttttaaaag ttttaagtct
    25861 atctatttgg caagtttaaa aaataaaaat tcggtatcaa aaaactgtta ccgataacat
    25921 ttttgaaagc gttgtctttt gcttctctat cacttatagt gatgacgtca tattaaaaca
    25981 caaggaggaa atcaatggat acagctgcaa tttatcatcg accggaaagt gaatatgcat
    26041 ttctatatac gaatacaaga tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa
    26101 aagtatatgt gctatgtgga gatccatata ccataacaac tgaaaaatgg tatcaaaaac
    26161 aacgaccaat gaaaaaatgt ctaagtacca atgttcatga ttattgggaa attgaagtga
    26221 cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt tggggaagat aacacggact
    26281 gtttttacgg tgatcaagga atttttcctt atcgagaaaa tgtgattact gaaccaaatt
    26341 tttattttcg aataccttat tttcatcaaa tcgatcgatt cactattcca gaatgggtaa
    26401 aagaaacagt atggtatcaa atttttcccg aaagatttgc aaacggggac aaagcaaatg
    26461 accccgaaca cactttacct tggggaagta aagatccaga ccgagaagac tttttcgggg
    26521 gagatttgca aggtgtgatg gatcatttgg attacttgac ggaccttggc atcaatggca
    26581 tctatttttg tcccatattt aaagccacat ccaatcataa atacgatacg attgattatt
    26641 atgaggtgga tcccgctttt ggcgataaaa aacttttaaa aaacttgatt gatgaagcac
    26701 ataaaagagg gatacgtatc atgttcgatg ctgtattcaa tcatatgggt gtccattcac
    26761 cacagtggca agacgttctc aaaaacggag aaaaatcgat ctataaagac tggttccaca
    26821 tccgtttttt cccagtagat tcttatcaga tgactgactt accagaaact gctgaaaata
    26881 tcccatatga tacttttgct tttactccat tcatgccgaa actaaataca gcaaatccag
    26941 aagtgcaaaa atatttgctg gatatcgcaa cttattggat aaaagaattc gatattgatg
    27001 gatggcgtct tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag
    27061 cagttacaga aatcaaacca gacatctata tccttggaga gatttggcat tcctctcaag
    27121 cttggctgca aggggatgaa ttccatgcag tgatgaatta tgcatttaca gattcgatta
    27181 aagattattt tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga atgaatcatc
    27241 agcaaatgct ttacagagat caggtgaacg aaggaacatt caacttatta gattctcatg
    27301 atactgccag gatcttgacc ctttgccaag gtaacaaaga attgatgaag tctgtgatgg
    27361 cctttatgtt tcttcaaaaa gggtctcctt gtatctatta cgggacagaa atagggatga
    27421 ctggtgaaga tgacccggat tgccgcaaat gtatgatatg ggagaaggag gaacaagatc
    27481 tagagctatt cggttttata aaagaacttg tttctctaag gaaacagttg tctaagatta
    27541 tttctgaagg ttctactcag tggctaatag taaatgatcg tgaagataag ctctatttca
    27601 cacggaaact agagggacag atcatctatg tttactttaa tcagtcaaaa gaaccttggg
    27661 tagtagaaca ggaaaatgag gtgattctta gtcaaaattg tcagttgtta gaagacggga
    27721 aagcagagat acgacaatac ggatttttga tcatgtgttg aaaactgtta ccggtaacaa
    27781 caagaaagcg tttgcaaatg aagatttgtg cccattataa taagggtgta acagcacgtt
    27841 gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt
    27901 aatctcttta ggaatcttag gcggattagc ggcttgtggc aatggtaact cttcagcaga
    27961 aggggaagcc gatgaaggaa aaacgttaac agtttccgtt gattctggat ataaagatta
    28021 tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga
    28081 aaaggacatg ttcgatcagt tggaatcatt agcgttagat gggcctgctg gaaagggacc
    28141 tgacgttatg atggcagcat atgaccggat cggtgcgtta ggacagcaag gacatctggc
    28201 ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca gacaaagccc aagtgactta
    28261 tgatggaaaa atctacggag aacctgcagt aatcgagacc cttgttttgt actataacaa
    28321 agatttagtc gatacagcac catcaacgtt taaagatttg gagaatctat ccaaagacag
    28381 tcgttttgct tttgagtcag aagcaggaaa aaatacagga tttttagcta aatggactga
    28441 cttttattat tcctatggtt taattgcagg ttacggtggc tatgtatttg gtgatgatgg
    28501 cacaaatcct tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc
    28561 aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga
    28621 cttcgccagc cagcaattca tgtccaataa aactgcagca atcattgacg gtccatggca
    28681 agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta gccaaaattc ctactctaaa
    28741 taatggacag ccttatcaac cgtttggcgg aggaaaagga tgggtagtca gcaattatgc
    28801 taaaaacaaa gacctctctc aaaaatggct tgattatgta acgaatcagg aaaatcaaga
    28861 aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa
    28921 ggctaaaaac gatgaattaa caacagctgt gattgaacaa tatgaaacag ctcaagcgat
    28981 gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc
    29041 agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga
    29101 agccatagaa caaaaatact gattaaaatg ataaccgtcc atttttataa aaaatggaac
    29161 aggtgcttca gataaaagtg gcaagagcga gatggaaaat tatcttgctc ttgttgccat
    29221 tatcttaaaa gcagtttgcg gtttagcaag tttagagggg gcattcagat gcagcaagat
    29281 aaaacaagca atatcaacga aaaaaaaatc acgctttgtt cacttattcc agggatgggg
    29341 cagtttgtaa atggccaagc ttttaaaggt atcgtttttt tcgccatatt cgtcctattc
    29401 attggtcaga tgcttctggg cggatttcat gcattggtag gattggttac tttgggaagt
    29461 ataccaatgg aagatcattc attatttatt ttgattgaag gcactttgca attcatcatt
    29521 acaatcattt tcttgatttt ttacggtttg aatcttttag acgcttatcg agtagcgaag
    29581 atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg aagtcataca taatataggt
    29641 gatgaaggtt ttccttatct attgacactt cccgcttatc ttctcatgat ttttacgatc
    29701 attttcccag tcctagttac attgttcact gcatttacta attatgactt caatcatatt
    29761 ccaccagcca gtttaattga ttgggtaggg gctaaaaact tttttagtat cttcttttta
    29821 agctcatatc gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt
    29881 gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca ccagaaattt
    29941 attaaattca aacgtatttt cggtatcatt ttcttattac catgggcggt cccagctttt
    30001 attactatca tgagtttttc gaatatattc aacgatagcg taggtgccat caatactcaa
    30061 gtgattcctt tcataaacca gcttcctttt gtagatatca gtgcaatctc ttggaaaacg
    30121 gacccgaact ggacgaaagt agcaattatc ttagtgcagg gctggttagg atttccctat
    30181 atctatgtga tggttacagg aatcttacaa tcgatttcag aagacttata tgaagcagca
    30241 aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc
    30301 ctagtggcag cgccaacatt tattacacag tatacgggga atttcaataa tttctccatg
    30361 atctatctgt ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg ttctactgat
    30421 attcttattt cctggatcta taaacttacg actggaactt ctccgcagta ttctatggct
    30481 tcagcaatca cgttgattat ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa
    30541 aagacaaatg cgttcaatat gggggattaa caatgaaaaa atttcattcg agtgctcgat
    30601 tccaaaaacg actttctcac ttttttactt acttttttct tttaatactg tcagtcatta
    30661 tcatctatcc gcttttgatc acagcaagta ctgccttcaa gtcaggaaat attgcagctt
    30721 ttagtttaga tctttcaagt gactggactt taggaaactt tactcggcta ttcagtgaaa
    30781 cactttacgg gacttggtat aaaaatacat tgatcatttc tgtcctaaca atgatcgtac
    30841 aagtgactgt agtaactttg gcgggctata cttatagtcg ctatcgtttt atcggccgca
    30901 aatcaagttt gatgttcttt ttagtgattc agatggtacc gactatggca gcattgactg
    30961 ccttttatgt catggctttc ttattagaag cgctcgataa gtactggttc ttgacaatga
    31021 tctatatagg cggtggaatt ccaatgaata cttggctgat gaaaggatac tttgacactg
    31081 taccaataga tttagatgaa tctgctaaac ttgatggggc agggcatttc aggatttttt
    31141 tccagattgt attaccgctt gtcaaaccga tgctcgccgt tcaagcatta tgggctttta
    31201 tgggaccttt tggggatttc atgttagcta agttcctgct tcgaacacct gaaaatcaga
    31261 ctgttgctgt cggcttacaa acatttattt ctgatgtgac aaaccaaaga gtctctcttt
    31321 ttgctgctgg ggcaattttg attgcactac cgatttgtct attattcttc tttttacaaa
    31381 agaactttgt taccggcctg acatctggcg gaacaaaagg ataggagcag agtatcatgc
    31441 aaacatttcc gttaaactat ttttccgcat tacgtacgcc aactcaagtg tttgcgggac
    31501 gcaaactatt atcatggcct aaatttttct tgatatttgt atttttagtt tccttgatgg
    31561 tcatgcctgt gactcttttt tatgccaatc agatccaagc gattccatta gaacaatttc
    31621 tatccgttca ttcgctgatc gatgaacaag gaacccaaaa attttctgaa cttgaattat
    31681 ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt gacccctgaa attttggtag
    31741 gtgtgtcttt atctgagaaa caacaatccg atcatggaac ttttattgat tttgagaagg
    31801 aacaatgggt gatccaacaa aaggataaat ctggtatacg ccgctatacg atgaattata
    31861 gcccatcttt ccaaccagat tctgtacgta cgcccgaaga tttccaacga tttttagaaa
    31921 gagaatttta tgccagtaat cgtccaacaa tcattttatc ttatagttta agtcttggtc
    31981 ttgtcctatt tgtcatgacg agtctcattt tattcggggc ttcctttttc ttgtggatga
    32041 caagaaaaag ccaactttca tccattcata cattcaaaga gagtgcaaat ttgatgctga
    32101 atgttatggg aataggttcc atggcagcag cagttgtagg gtttatccac tttgacttta
    32161 tcttgatgct aagtgtccaa acaacgataa ctgtcttgct gcttttatgg gtttttgtca
    32221 aaacaagatt caaagataag cgtgtcgaat gacaagttta agcgatttat gcgacaatga
    32281 atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct
    32341 tagttgattc ttttcttttt tagtattaac gtaatagggt agttttttta aggaggaaaa
    32401 tcatgttaga gaaaacttta tacaatcaat tattttcacg ttctttctcg ctccctgtcg
    32461 aagtaaatta ttgggacggt acaacgaaac aatacggaga tacggacact cctccccaaa
    32521 tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat tacaagtaac gcatctttgg
    32581 cactaggaga agcctatatg gatcatcgaa tcgagatcga gggaagtatc caagcattga
    32641 tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg aaaagattat ctaaaatggc
    32701 tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga agatatccat gcgcattatg
    32761 acttaggtaa cgacttttat aaactttggc tagatccaac tttgacttat tcttgtgctt
    32821 atttcaatac acctgaagat acgttggaac aagcacaaat caataaagtc catcatatct
    32881 tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa
    32941 cattgatgtt cactgctacc aaagagtata atgtcaaggc aactgggatc acactaagtg
    33001 aagaacaata cgatttgatt accaaaagaa taaaagaaga acatttagaa gataaatgcc
    33061 gtgtattatt aatggattac cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg
    33121 taggtatgtt cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag
    33181 atttattgaa accaaaagga acagctttga tccatggaat cagccgacaa caaggcggtg
    33241 cgacaaatgc ttggatcaat aaatatattt tccctggcgg ttatgtccct ggtgttactg
    33301 aattagttga taatattaca aaaaacgatt tgcaactgat tgatttggaa agcttacgtc
    33361 gtgactacca attgacatta gaacattgga caaagaattt tcatcaagtc caagatcaag
    33421 tcacgaaaga aatgggtgat tccttctatc gaatgtggga tctatattta caagcttgtg
    33481 cagcttcatt tgaatcaagt aatatcgatg tgattcaata tttgcttgtt caacctggaa
    33541 acaatgacat cccaatgcat cgtgcataat tgaggcaaaa gtactgttta aagactattt
    33601 aaaaagaagc taagttttat gatgctccag aagtgaactt ttttgtctaa cttctaggat
    33661 tgtctctaaa gcttagtttc tttttcttta tattttcata ataaaagttt ggttctttaa
    33721 tttttaaaca tgacagaaaa ttttttcttc gttatacttg taatagaata ctattttata
    33781 acaaagggga tcgaatcaat gaaaaaaact gttatttata ctagtttatt tgcagctttg
    33841 tttttagctg cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt
    33901 agcgaacagc gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct
    33961 agccagacaa aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct
    34021 cgtgcgatcg aattgttcca agataaatac ccagatactg caattactag tttggaatta
    34081 gatagtgatt ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac
    34141 gaagcaagaa ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat
    34201 cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc
    34261 acgattgagg aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat
    34321 tgggatttgg ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat
    34381 caaagtacat cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat
    34441 gattaagaat ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac
    34501 aaaataactt gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata
    34561 tctttgccaa tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag
    34621 atgaactggc tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac
    34681 gaaaaggaaa tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg
    34741 gacttccaaa cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc
    34801 aggaaaacca aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact
    34861 tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat
    34921 cttgtcccaa tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa
    34981 cactgaaaga agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt
    35041 ttgattttgc tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg
    35101 tcaatgctat caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag
    35161 tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt
    35221 tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag
    35281 gtgggattcg gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac
    35341 agattggtac cgagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat
    35401 taacgcagat tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac
    35461 gcaccatttc atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa
    35521 acactcgtct gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga
    35581 aaataacaga ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct
    35641 taaagaaaaa gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa
    35701 gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga
    35761 gctattgatt ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat
    35821 gctcggtcaa gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca
    35881 ccgagcttct catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt
    35941 tggacaaacc aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg
    36001 cccagcaaga aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc
    36061 tgctgcagtt tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat
    36121 tagaataccc gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc
    36181 agttggtcca tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt
    36241 ttctatgaga gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc
    36301 atatcaagta tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc
    36361 tttttgtgtt gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa
    36421 aaaagctgta gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact
    36481 agatggtcgt aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat
    36541 gatccgctcg attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat
    36601 ttcttggact ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct
    36661 gcatgtatgg gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt
    36721 cctttcacca acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc
    36781 gataagagca cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt
    36841 ttcttccatg tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac
    36901 tggacaacca gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat
    36961 ccaatttatg gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga
    37021 agcaaatcat cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga
    37081 atgaagaaaa aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc
    37141 ggtacagctg ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa
    37201 ttagtagtag cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa
    37261 ttagttggtt atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc
    37321 tcctttaaag aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat
    37381 tttgctgcga acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag
    37441 actttcaaat attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac
    37501 tcacttgaag atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt
    37561 ttagcggaaa aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat
    37621 ttaagcgatg tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa
    37681 atggccgtat ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat
    37741 gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat
    37801 ggcgtattaa aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt
    37861 caaactgatg tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt
    37921 actttcctct aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct
    37981 tggtatacac attagggatt ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc
    38041 ttttgacagt tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt
    38101 tatcgttctt tagaggcatt cccgcactag tcctgctgtt tctgttatat tttgggttac
    38161 cttatcaatt aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat
    38221 ttattggtga aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg
    38281 gtttagcttc tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt
    38341 ttcgaatctc agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt
    38401 tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga
    38461 cattcgatta tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg
    38521 ggatcgaaaa agtgcaacag tatttagaaa aacgttctat gcatcagtca atctgaaaaa
    38581 tagaaatggt gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc
    38641 tagtatttta aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa
    38701 catgaagacc ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca
    38761 aggcggcttg gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct
    38821 tagctgcttg acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac
    38881 aaaagcagat tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt
    38941 tttaccaagt aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt
    39001 agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc
    39061 gaaaaaagca gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat
    39121 ggcttcttta ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt
    39181 tccaacagat caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc
    39241 taacgaacga tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt
    39301 gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat
    39361 ccgtcggttg cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga
    39421 aaacaatttg acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa
    39481 atccaaccag tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta
    39541 tttcaaaaaa agcaccatcg aatcgctgtg atccaaaatc cccaagtagg acaattcaaa
    39601 ttattcgatc agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca
    39661 cagatcttaa cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt
    39721 ttcgatggac agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat
    39781 tcgaaatttt tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt
    39841 gtgatccgtg actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat
    39901 gactttttta gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat
    39961 ttcacgatcg aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc
    40021 catctaacgt atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt
    40081 aacgactctg ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa
    40141 tggatcatgc gtcaccatca tcaactcttg attacttcag gaatgattga attattggct
    40201 ctttaccagc aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat
    40261 catttggaac ttattagtca cctgctagat catccaaaag attggcttaa atattattca
    40321 aaagttcagg aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata
    40381 gaaaagagga aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt
    40441 agaaatcatt aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa
    40501 cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt
    40561 attaatagtg gaagatcaaa ttgctggaac agctgcatta gtctcaagcg tggatcccgt
    40621 ttacacaaaa atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg
    40681 tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt
    40741 gttgacgatt gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa
    40801 tgaaccgatg gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt
    40861 tccttttgaa cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca
    40921 tctgagcaaa gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag
    40981 gttgtaacag aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa
    41041 atttttgtgg aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg
    41101 agaggtcgtg acatagcttt tgtcacgacc tctcggctta tcctacgagt attattcgat
    41161 acatttatat caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt
    41221 ttgacacgac taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta
    41281 cataaaccgc cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg
    41341 attgcaatgt ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact
    41401 catataaatg ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt
    41461 tctttttaat ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg
    41521 caaactcatt tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct
    41581 cttgatcttt ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg
    41641 gctgaaaaat agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat
    41701 agcaataatt cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa
    41761 tatttccaaa gcttttaaaa gtaactttgc ttttttgtat attataccag caagccagtc
    41821 acaatagcac ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt
    41881 gcgacaagat ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata
    41941 gaagagaagt tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta
    42001 aaatggaaat taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta
    42061 gtcgccatga tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca
    42121 agaggggcaa agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag
    42181 atagcattga tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc
    42241 gccacatgga aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg
    42301 ttttcttcaa tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa
    42361 gccagaatat aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg
    42421 atcagtgcca tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc
    42481 atggtgaaca aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact
    42541 tccgattgtc ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg
    42601 ttgattttac ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag
    42661 cgtagaatac cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa
    42721 ggtgcacttc ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa
    42781 gcaagaagat gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta
    42841 cgtaataata cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat
    42901 ttgattgctt ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca
    42961 ataataccta ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc
    43021 tgttttgcaa ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac
    43081 gcattcattt tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt
    43141 cttagtaaag tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca
    43201 tttataagta aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca
    43261 ggtaatatcg attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt
    43321 agagtgggct tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg
    43381 attcgttact gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa
    43441 agaggaatga agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa
    43501 taaaggaatg tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc
    43561 tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat
    43621 tgcaatcaca gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc
    43681 atgggcaggg actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag
    43741 tttgattcga ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg
    43801 gaaataacga cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat
    43861 cccaaatcct acacgattca cacccatgtc agtaggagat tgataaacag attctttacc
    43921 agtaattttt tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt
    43981 cacagctact ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag
    44041 atcgactgtg gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac
    44101 tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag
    44161 ttggttcaag gaagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat
    44221 tggtttagtt gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc
    44281 tacgttgact ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc
    44341 tagtttattg ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact
    44401 gttgattaat aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata
    44461 agtaatccca taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat
    44521 cagtatttca gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa
    44581 tcctggaagt acacgtttcg catgcttgtc gtcaactagc ttcccaccaa attctaaata
    44641 taatttgtca tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt
    44701 ttctgaatca aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt
    44761 catttttttg aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg
    44821 gtttaaacca acttgtctag atagttattc attggtatga tagtttttga taaggaaggt
    44881 gctacatgaa aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt
    44941 ttaccggtta tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc
    45001 taaacgaaga aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag
    45061 atctgcctag taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga
    45121 tggaaagact agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg
    45181 gagcattgcc cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt
    45241 aataagtgaa aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat
    45301 gcaaattatg agccagacga actagaggag acgaatgcgg atcaggatac aggacaagaa
    45361 tcgtcttctt tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa
    45421 gacttacagg cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta
    45481 tacaatggcc gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat
    45541 caattgctga tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa
    45601 gtagaaaaca tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg
    45661 cccacggtcg gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa
    45721 gagtgggaga aatattccca accggagtca acggactatg acaaaaaaat caatgagcta
    45781 gacgcaaagg tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa
    45841 atcaatcgac aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc
    45901 gaggaagaaa ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc
    45961 tggcttcctt atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa
    46021 gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga
    46081 cgctatttcg gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat
    46141 cttggcaatg agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca
    46201 cgatgaagca gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac
    46261 ttcattggaa gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga
    46321 ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa
    46381 cacccatgct tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca
    46441 tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag
    46501 aaaagatttg atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt
    46561 gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga
    46621 attactgaaa ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg
    46681 gaaaacaaga catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc
    46741 ctttgtatta ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt
    46801 atcaccagag acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca
    46861 ctggtcagtg gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa
    46921 tcaattatta gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa
    46981 tacgaagcca cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga
    47041 tgatattttt atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac
    47101 tacttatcaa gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga
    47161 tcaaacagtc ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat
    47221 caattacgaa ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta
    47281 ccagatgtca ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg
    47341 tcacaagtct tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat
    47401 ttcattagga atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt
    47461 agcaggtagg atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt
    47521 atttatagta ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc
    47581 gatcagcagt ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac
    47641 tgttgttctg agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg
    47701 gttaaaggga gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt
    47761 tgatacgctg ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt
    47821 tgatttgatg cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa
    47881 acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa
    47941 tgagtgtcag tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca
    48001 accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc
    48061 aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg agtattttga
    48121 aacgaaattt tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac
    48181 aggatgagaa aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg
    48241 gtgccaaaga gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat
    48301 aagtttcata aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat
    48361 gaaagaagga aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg
    48421 ctctaggggc taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca
    48481 gtgccgccat gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc
    48541 tgttattcgg tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag
    48601 gacgagcaaa atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg
    48661 cacttggggt gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc
    48721 cgatcattgt tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg
    48781 tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc
    48841 gcgaaagtcg acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg
    48901 gtttggtctt ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg
    48961 atgcgatcag cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa
    49021 aagaatttta tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca
    49081 agttagcttt tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa
    49141 gtccaacgga gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag
    49201 cttactcggt catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga
    49261 tgtatatata cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt
    49321 attcatgaat tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac
    49381 aatttagcga atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata
    49441 ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac
    49501 tttgtataag ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat
    49561 ttgatcctct cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt
    49621 tagttgcatc tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct
    49681 ttttagaatt acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa
    49741 agcggacgtt tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact
    49801 acttaaagtt ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt
    49861 tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc
    49921 gagtagatgc tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca
    49981 tgcagatgaa aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg
    50041 tattcatggt attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat
    50101 ttgccggatt tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc
    50161 gcggtaaaga tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg
    50221 actaaactat atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc
    50281 ttgttcccga tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg
    50341 aaatatgtac cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat
    50401 gaacatctgg atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg
    50461 gcttacaaaa cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg
    50521 ctgtctatgc tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc
    50581 aaactgatct atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag
    50641 ggataaaaaa gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag
    50701 agggatttgt ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga
    50761 atagttgagg gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt
    50821 gctaatcttc tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg
    50881 gggaacaatc aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat
    50941 ggattgttcc tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct
    51001 gtagccacaa aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat
    51061 atcatgtttg tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta
    51121 gccggaatca gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc
    51181 ccatctttga ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat
    51241 gatctgcggc cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc
    51301 ttggctggta cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg
    51361 atcagtacag tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc
    51421 ggacgcagaa aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa
    51481 agagaaaacc gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc
    51541 ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac
    51601 tttttccgtc cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc
    51661 aatccaaata aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc
    51721 ttgccaatat taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga
    51781 gatagttttt cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta
    51841 gctggaccac tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa
    51901 atggctttgc tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa
    51961 tctttgaatc accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc
    52021 atcttccaag taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc
    52081 ttagcattcg gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata
    52141 gcacctttag caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg
    52201 cttgcagtat gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa
    52261 agctatgcaa tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt
    52321 tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc
    52381 aaagaatagg ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt
    52441 acgcttgtcg tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata
    52501 gttataaggt ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta
    52561 gttacagata gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt
    52621 attccaatcc ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg
    52681 gatgaatatt atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc
    52741 gtaggggaag ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta
    52801 agtatccatc tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat
    52861 gacatcagcg gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga
    52921 cacatggtag aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt
    52981 gcacatatcg atcgaatccg agataatacc tacgcttatc tgattgtaga tgatttgaac
    53041 aatttagtac gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa
    53101 atcaaaccga tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca
    53161 agtaaaaaag catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa
    53221 ccgccagtca aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa
    53281 gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc
    53341 attggtacac atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt
    53401 ttattcaaaa aacataccaa aaatattcca acatcatctt ggactttcta catgttacta
    53461 atgagcgtgt aaaagaacga gatggtgttt ttatttctat caatgaagct ctttttcttt
    53521 gcaatcctca cttcaatcct ctataatgtc gcatatagtt tagtaaatga gtaaactact
    53581 aatttactta tttactaaaa ataaaaggat gaggagttaa aataatggaa ataccaacaa
    53641 atttaaaaca taaaccagta atcgttgtag aagactatga taaagttgat ggaagaaatg
    53701 cattaaatac ggatgcaaaa gggctttcac tgggccttgc tcagtggaat gatcgtggaa
    53761 aagtcgatat ctcaggcaag gtatggcgac atactggaga aaaatggtca cgacagtcgg
    53821 aagaattacc aatcacacgt attttagatt tagcgatctt agctgcacaa ggaagtttat
    53881 acttccaaga tgcttatcgt tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca
    53941 tcattggatt acaagggaac cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg
    54001 atgaagacat cattttgtat tacgatacac tacaaaaaga cggagaactg attggggaac
    54061 gattccgtat tttaaaacgt cttttagatg agctaggata ttaatagaca gagatgatca
    54121 aaaggggaag agaagcatgg agaatttctc actaacagaa aaccgaacct tttcacggct
    54181 agaaaagcaa atgatctgga agaaaccgaa aagccatcgg acaagtgcag aagaatggcg
    54241 gatcgcctcc gaaatcaaag ggaattggca agatcctgaa atgaaaatca gtaacgtact
    54301 tttggaagga gatgccgggt cggggaagac acaattagcc aaagcactat ctgctgatct
    54361 gcaacttccc tatacaaaag tgacctgttt tgcagatatg gacaaatcag atgttttcgg
    54421 agccttatta cctgtagtag attctaataa ggaacaagac caagaattac tagaagctat
    54481 ttaccaaaca gatacacttg aagctgtact gacactgatc cagaaccatt acggcattga
    54541 tcctttttct gctaaagaaa aattagcaga gctagttcaa aggatcgaag ctgattatcc
    54601 agatgcaatc cattacagct attacccttc tgaaatcgtt cgagcgattg aaaaaggata
    54661 tttattagag attcaagagc cgactgtcat ccgagatgcc tctgttcttg tggcacttaa
    54721 ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc
    54781 agattgcgtc attgtgatca ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga
    54841 atctttaaga gatcggatgc aacatgcgga aaaaatggat cttcctgcct tagaagtcat
    54901 ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa gaacttctct taaagatggc
    54961 agagattatc cgtttgttgg atgaaactgc aaaagctaac gccatcaaag gtgtagcagg
    55021 aatgcgttct tatttttatt gggcaaatac ctataagcaa ggacaagatt tactccagtc
    55081 gatttatcca aaagttttgt ataaattatc cactgattca gatgaattgg caattcttga
    55141 acaagctttg tctgacagtg gattgctact ggaacttcag gacatattga gaaaagaaaa
    55201 atggggtcat cctcaatctg agcatacaaa aggaagaacc attagcgccg aagaagcaaa
    55261 tgaacgaaac atcactgaaa atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt
    55321 gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa gaaacaatag aaaaaaacga
    55381 gattcctcct caaatgcaaa aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga
    55441 acaacaaaca ggagatgatt ctcaagaaat gtctgcttct gacatggaag cacatgataa
    55501 ggaaatcaag aagcaactga ataaagaagc gcgacaaatc atgaaggaaa cgattcatga
    55561 aaaagaaggt ctcatcgttc accgaccagc tttccaagga aaaacgccgg aagcacgtga
    55621 gctacacatg caagtaatgc caatagtaga atcacttagc cggcaaattt tagagttact
    55681 ggataatgaa caaacagaga cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc
    55741 aagcaaagta gcatatggtg atttgagaaa ttttgacaaa aagaacccgc cacatgagca
    55801 accttcttta gcggtagctg tacggatcga tgaatctggg tcaatgatac gggatgatcg
    55861 gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa ttttctaaga aagtcgatat
    55921 cccattgttg atttacggag atacagcaga ccgttcgtca agagaaaaga cttctttgtt
    55981 ttcttataaa gaatttgaag acggatttca atggttaaat gaaaaattag tcactatgaa
    56041 accaaggcaa aacaatcgtg atggtgctgc acttcgttta gcagcagaaa aattaaacag
    56101 acaatccgca acaaccaaac tgttgcttaa tattagtgac ggtcagccaa aagcgttgcc
    56161 ggattataca ggcaaaaaag caaaagaaga tatccagtct gttttgaaag aatatgaacg
    56221 tcaaggaatc ctatttgtct ccgcagctat tggccaagac aaagaagaaa tcaagtcgat
    56281 ctacggtgaa agtcgttttg tagatattac tgacttgaat gaatttccca agcaaatgat
    56341 tcaactgatt gcccgctatc tatgataaaa taaagctcaa cgaacgcact tagcagattc
    56401 gttgagcttt tcgtttctag ttaccggtaa caaggatact tctaaaatag aatcggttat
    56461 aatcaagagg gaccgttaaa ggagtgaaac gatatgatac aagaacaaaa atggtggaaa
    56521 gaagcagtag gctatcaaat ctatcctgct agtttcaaag atagcaatgg cgatggtcaa
    56581 ggagacatca atgggatccg tgaaaaactt aattatttga aagatcttgg tatcggcttt
    56641 atctggatca atccgatcta tcaatctcca tttgtcgata atggttatga cgtaagtgac
    56701 tatcaagata tagaagagag attcggaacg atggaagatt ttgatttatt gctaaaagaa
    56761 gcacatgaat tagggatcaa agtgattatg gatcttgtca tcaaccatag ttcggatcag
    56821 catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt
    56881 tgggtcgacg gggtagatgg caaagaaccg aataattgga catcgatttt cggcggttct
    56941 gcttgggagt attctcatga aactggacag tattatctgc atgtttttgc aaaagaacaa
    57001 ccagacttaa attgggaaag cgagaaattg aaagaagaac tattcaatat gatccgatgg
    57061 tggttggata aagggataga tggatttcgt ttagatgcga tttctcatgt aaaaaaagat
    57121 gaatattcag taaaagccac agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc
    57181 gaagaacatt tgaccgattt gaaacatgtt tttgaagagt acgatatcat gactgtcgga
    57241 gaagcaagtg gtgtcactgc agaagaagga ccacaatggg tagggaaaga cggttatttc
    57301 gatatgatat tcgaatttga tcatatccat atctggcagc aagaaaaaga agggcaatta
    57361 gatgtcctta aattgaaact tgcattaagc gcttggcaaa cttcattgga tggtatcgga
    57421 tggaacgctt tatatatgga aaaccatgat gttcctcgtg ccgtttcagt gtttggagat
    57481 actcgtcccg atttttgggc tatgtctgca aaagcaatcg ctatgatgta tttctttttg
    57541 caaggtacgc catttattta tcaaggacag gaaatcggaa tgacgaatat gccctttgaa
    57601 tcgatcgatc aagttgatgc agtagattcg aaaagacttt ataaaaggct gcttgcggaa
    57661 ggtaagacaa gagaagaagc attggatatc attcgtgaaa caacgagaga caacagcagg
    57721 acacccatgc aatggacttc ggaacaatat gctggttttt ctacacatga accttggctg
    57781 atcactaatc caaataccaa aacgatcaat gtagaacaac aagaatatga accagagtcg
    57841 gtcctgcaat tctataagaa catgatccgc attagacaaa ccaataaagg tctgatatac
    57901 ggttcttata aagaatatct tcatgaacat ccacaacttt atgtctatga aagatattta
    57961 gaagatgaac aatatcttat tatggtaaac ttaacagaat cacttgctga ctatgaactt
    58021 ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa attcctctag cggagaattt
    58081 gaagctaaag gcatactagc accttatgaa gctcggttat ataaaacaaa caagtaaaac
    58141 ttcctcgtta aagttcatta gttggacttt gcgaggaagt ttttcagatt gctctgtcaa
    58201 ttactcattt caataattcg ctctaggacc accgatatat ttaggacgag agcgaagggc
    58261 tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg acaggtactt ggacatgttt
    58321 gatatgcata ccgattgatg tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc
    58381 cacttcgaca ggatcattga actggtcaaa agcagcaaca gaacaagcac ctccagcatg
    58441 tagtgcagga acaacagaga caatttcgaa attttttgct tctgccactg ttcgttctac
    58501 tactaatgct cggttcaaat gttcacaacc ttggacagct agcgcaattt ctttaggatc
    58561 caacaactct tttaacgtac ggatgatcca ttgaccaact tccgcactgg aattttttcc
    58621 aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa acaaaaagat cacctttttt
    58681 caagtgtgcc tcagccaata tgtcattaac tatttcagtc aattggtctt ttaattcttt
    58741 ttctgacaac ttcattttct aaaaccttct ttttcaatta atattgcttc cgtgcttgaa
    58801 tacgttgaag acttgcagtt ataggaaagg caagtcccat tccaatcaga ttttggaaga
    58861 tgtttcctgg tatagaagcc actccagccg gccaatcata taaaatccaa ccggcgatac
    58921 catagccgat gatcataact aagcttccta ataaaagccc taatccccac atacgagtag
    58981 aagatttgct gaaagaacca gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa
    59041 ctgcccactg cggatatcct gaaatcaaat cgatcaatag accactggaa gcaccaacaa
    59101 ctaatccacc cgttggacca taaagaaaag cagcggtata gatcccggcc tcgcataatg
    59161 tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt taatgaaagg gcaactgtca
    59221 acgcaatcag catagcatag atcgttaatt gcttagtttt catttctcat tcctcctttt
    59281 tgaataccgc cagaccagac acttccggta tgatcgttca tcagcacgcc attagaaata
    59341 caatgggcaa caaatttttt actttctgct actgctttgg taaaatctgc ccctaacaca
    59401 agattcgctg ttattgcaga agaaaaggca caaccggccc cgtttactgt tgtagtgtcg
    59461 atttttttat gagaaaatac ctcataatga tcaattgaat agagaagatc cacagcttca
    59521 tctccagcca tccttgtgcc gcctttgatc acaatgtttt ttgttcctga agcagtgagt
    59581 tctttagcga gatggatcat ttctgttaac gaagagggaa gagcttgacc agaaagtaaa
    59641 gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc
    59701 tgcgaaatat acaattgatt gatcacagct tcagtttctt tgaatgccaa aacagggtct
    59761 aaaatgattg ggatattttg ttttgttttt atgaagtctc gaacaaccat gattgcttca
    59821 cttgaattca ataacccaat tttgatacca tctaatgaag tcattttatc aatcgaaact
    59881 aactgtcgtt taagcaattc caaagatata gattccagca taaatcgctc attaatatct
    59941 agtgccccta tagaagtcaa ggcgcttagt ccgaatagac caaaattctc aaaagttttt
    60001 aaatctgttt ggattcctcc accagcaaat ggatcagaac cgccaattgt caagattctt
    60061 ttcataacat tttcctccat gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc
    60121 caattggcat atttttatct catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa
    60181 tggatctttc tttccttaaa tagttaggac ttttgtgatt ttaattcttt ctcgatttga
    60241 agccgttgca ctttcattgt agctgttcgc gggattgcat caaaatcacg gatgataggg
    60301 acgttcaagc gaggaagttc atggacttgc tcccaccatc gattccaatc catttcttta
    60361 tcaggagcta aagcaataat cggttgtgga gaatgatttt tatcccttac aatcactact
    60421 tctgccaaaa aatctaacga atctaataaa aagtcttcaa tagctaatgt actattgatc
    60481 gtttcgatca aatccacttg ccgatctttc aaaaataggt ggccatgctt atccataaaa
    60541 ccgtaatctc cgctatccca ccaatcgcca taaacatttt cctggaaccg tgcatcttcc
    60601 ttgtaataag tcaaagcccg tcctttagac agtaattgta tatgaccatc tgtgttttca
    60661 ggtaggacat tcccaactga atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg
    60721 cccatatctc ttgcatcact tgttttcaaa ctctctagtg tatgagcctt aagaatcatt
    60781 ggtccgcatt cactttggcc atatacttga agaaataccg catcctgtgc catcgacgtt
    60841 cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa aagtggagtg atagtatcgg
    60901 atacccgaaa aagcttctgg atgctcctta gcagtaaaac gccactgaac aaagttattc
    60961 ggatgggtct ctagtgcaat tggccggtga gcttccaaca tatgaacaac ttttgaactt
    61021 tgtgcgtttg ctaaaggcat cataggaaat cccatggaca tcaaagaaga gataccaata
    61081 ttgaatctgg aatgcactgg agagatgtga aaagcgatta atttcttttc cgaaattttt
    61141 gtgaaaactg ttttttgcca tttcgtccgc catcccatag aatgagcaga atgacaaata
    61201 agtttaggga taccagttgt accagaagta tgggtcatat aagaaatttc atttttgtct
    61261 aaaaatgatt gagaaacaga caaagcaggt tgttccatta aataaagaat agga
//
LOCUS       DACEDM010000005        58525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEDM010000005 DACEDM010000000
VERSION     DACEDM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.005"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            255..686
                     /locus_tag="IWO50_000309"
     CDS             255..686
                     /locus_tag="IWO50_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0357461.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            693..926
                     /locus_tag="IWO50_000310"
     CDS             693..926
                     /locus_tag="IWO50_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0357462.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(848..1090)
                     /locus_tag="IWO50_000311"
     CDS             complement(848..1090)
                     /locus_tag="IWO50_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357463.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            1347..1550
                     /locus_tag="IWO50_000312"
     CDS             1347..1550
                     /locus_tag="IWO50_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0357464.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            1634..2827
                     /locus_tag="IWO50_000313"
     CDS             1634..2827
                     /locus_tag="IWO50_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0357465.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            3005..3235
                     /locus_tag="IWO50_000314"
                     /pseudo
     CDS             3005..3235
                     /locus_tag="IWO50_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            3363..4046
                     /locus_tag="IWO50_000315"
     CDS             3363..4046
                     /locus_tag="IWO50_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0357466.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            4355..4428
                     /locus_tag="IWO50_000316"
     tRNA            4355..4428
                     /locus_tag="IWO50_000316"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4389..4391,aa:Arg,seq:tct)
     gene            4587..4946
                     /locus_tag="IWO50_000317"
     CDS             4587..4946
                     /locus_tag="IWO50_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0357467.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            5069..6700
                     /locus_tag="IWO50_000318"
     CDS             5069..6700
                     /locus_tag="IWO50_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0357468.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6762..7403)
                     /locus_tag="IWO50_000319"
     CDS             complement(6762..7403)
                     /locus_tag="IWO50_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0357469.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7685..7993
                     /gene="rplU"
                     /locus_tag="IWO50_000320"
     CDS             7685..7993
                     /gene="rplU"
                     /locus_tag="IWO50_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0357470.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            8020..8364
                     /locus_tag="IWO50_000321"
     CDS             8020..8364
                     /locus_tag="IWO50_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0357471.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            8377..8670
                     /gene="rpmA"
                     /locus_tag="IWO50_000322"
     CDS             8377..8670
                     /gene="rpmA"
                     /locus_tag="IWO50_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0357472.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            9077..11911
                     /locus_tag="IWO50_000323"
     CDS             9077..11911
                     /locus_tag="IWO50_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0357473.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            12102..12593
                     /locus_tag="IWO50_000324"
     CDS             12102..12593
                     /locus_tag="IWO50_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0357474.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            12696..13685
                     /gene="manX"
                     /locus_tag="IWO50_000325"
     CDS             12696..13685
                     /gene="manX"
                     /locus_tag="IWO50_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0357475.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            13718..14521
                     /locus_tag="IWO50_000326"
     CDS             13718..14521
                     /locus_tag="IWO50_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0357476.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            14542..15456
                     /locus_tag="IWO50_000327"
     CDS             14542..15456
                     /locus_tag="IWO50_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0357477.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            15539..15913
                     /locus_tag="IWO50_000328"
     CDS             15539..15913
                     /locus_tag="IWO50_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0357478.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            16052..16297
                     /locus_tag="IWO50_000329"
     CDS             16052..16297
                     /locus_tag="IWO50_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0357479.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            16469..17533
                     /locus_tag="IWO50_000330"
     CDS             16469..17533
                     /locus_tag="IWO50_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0357480.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            17779..18204
                     /locus_tag="IWO50_000331"
     CDS             17779..18204
                     /locus_tag="IWO50_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0357481.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            18197..18646
                     /gene="nusB"
                     /locus_tag="IWO50_000332"
     CDS             18197..18646
                     /gene="nusB"
                     /locus_tag="IWO50_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0357482.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            18743..19597
                     /locus_tag="IWO50_000333"
     CDS             18743..19597
                     /locus_tag="IWO50_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0357483.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            19598..20935
                     /locus_tag="IWO50_000334"
     CDS             19598..20935
                     /locus_tag="IWO50_000334"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0357484.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            20940..21164
                     /locus_tag="IWO50_000335"
     CDS             20940..21164
                     /locus_tag="IWO50_000335"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0357485.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            21166..22056
                     /locus_tag="IWO50_000336"
     CDS             21166..22056
                     /locus_tag="IWO50_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0357486.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            22065..22883
                     /locus_tag="IWO50_000337"
     CDS             22065..22883
                     /locus_tag="IWO50_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0357487.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            22990..23439
                     /locus_tag="IWO50_000338"
     CDS             22990..23439
                     /locus_tag="IWO50_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0357488.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            23457..25139
                     /gene="recN"
                     /locus_tag="IWO50_000339"
     CDS             23457..25139
                     /gene="recN"
                     /locus_tag="IWO50_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0357489.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(25194..25310)
                     /locus_tag="IWO50_000340"
     CDS             complement(25194..25310)
                     /locus_tag="IWO50_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0357490.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(25383..26339)
                     /locus_tag="IWO50_000341"
     CDS             complement(25383..26339)
                     /locus_tag="IWO50_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0357491.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            26510..26878
                     /locus_tag="IWO50_000342"
     CDS             26510..26878
                     /locus_tag="IWO50_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0357492.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            27048..27479
                     /gene="mraZ"
                     /locus_tag="IWO50_000343"
     CDS             27048..27479
                     /gene="mraZ"
                     /locus_tag="IWO50_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0357493.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            27500..28459
                     /gene="rsmH"
                     /locus_tag="IWO50_000344"
     CDS             27500..28459
                     /gene="rsmH"
                     /locus_tag="IWO50_000344"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0357494.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            28464..28865
                     /gene="ftsL"
                     /locus_tag="IWO50_000345"
     CDS             28464..28865
                     /gene="ftsL"
                     /locus_tag="IWO50_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0357495.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            28866..31058
                     /locus_tag="IWO50_000346"
     CDS             28866..31058
                     /locus_tag="IWO50_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0357496.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            31084..32046
                     /locus_tag="IWO50_000347"
     CDS             31084..32046
                     /locus_tag="IWO50_000347"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0357497.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            32060..33421
                     /locus_tag="IWO50_000348"
     CDS             32060..33421
                     /locus_tag="IWO50_000348"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0357498.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            33447..34535
                     /gene="murG"
                     /locus_tag="IWO50_000349"
     CDS             33447..34535
                     /gene="murG"
                     /locus_tag="IWO50_000349"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0357499.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            34550..35770
                     /locus_tag="IWO50_000350"
     CDS             34550..35770
                     /locus_tag="IWO50_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0357500.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            35926..37251
                     /gene="ftsA"
                     /locus_tag="IWO50_000351"
     CDS             35926..37251
                     /gene="ftsA"
                     /locus_tag="IWO50_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0357501.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            37274..38515
                     /gene="ftsZ"
                     /locus_tag="IWO50_000352"
     CDS             37274..38515
                     /gene="ftsZ"
                     /locus_tag="IWO50_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0357502.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            38532..39209
                     /locus_tag="IWO50_000353"
     CDS             38532..39209
                     /locus_tag="IWO50_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0357503.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            39223..39822
                     /locus_tag="IWO50_000354"
     CDS             39223..39822
                     /locus_tag="IWO50_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0357504.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            39837..40124
                     /locus_tag="IWO50_000355"
     CDS             39837..40124
                     /locus_tag="IWO50_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0357505.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            40173..40955
                     /locus_tag="IWO50_000356"
     CDS             40173..40955
                     /locus_tag="IWO50_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0357506.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            40976..41683
                     /locus_tag="IWO50_000357"
     CDS             40976..41683
                     /locus_tag="IWO50_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0357507.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            41945..44743
                     /gene="ileS"
                     /locus_tag="IWO50_000358"
     CDS             41945..44743
                     /gene="ileS"
                     /locus_tag="IWO50_000358"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0357508.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(44792..46318)
                     /locus_tag="IWO50_000359"
     CDS             complement(44792..46318)
                     /locus_tag="IWO50_000359"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0357509.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDVFEWIWQPDNWYRERGKLND
                     "
     gene            complement(46333..46980)
                     /locus_tag="IWO50_000360"
     CDS             complement(46333..46980)
                     /locus_tag="IWO50_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0357510.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(47164..47493)
                     /locus_tag="IWO50_000361"
     CDS             complement(47164..47493)
                     /locus_tag="IWO50_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0357511.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            47670..48398
                     /gene="sfsA"
                     /locus_tag="IWO50_000362"
     CDS             47670..48398
                     /gene="sfsA"
                     /locus_tag="IWO50_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0357512.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            48414..49427
                     /locus_tag="IWO50_000363"
     CDS             48414..49427
                     /locus_tag="IWO50_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0357513.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            49427..50704
                     /locus_tag="IWO50_000364"
     CDS             49427..50704
                     /locus_tag="IWO50_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0357514.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            50767..53469
                     /locus_tag="IWO50_000365"
     CDS             50767..53469
                     /locus_tag="IWO50_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0357515.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            53621..53938
                     /locus_tag="IWO50_000366"
     CDS             53621..53938
                     /locus_tag="IWO50_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0357516.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            53968..54288
                     /locus_tag="IWO50_000367"
     CDS             53968..54288
                     /locus_tag="IWO50_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0357517.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            54396..55856
                     /locus_tag="IWO50_000368"
     CDS             54396..55856
                     /locus_tag="IWO50_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0357518.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            55924..56145
                     /locus_tag="IWO50_000369"
     CDS             55924..56145
                     /locus_tag="IWO50_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357519.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            56176..56358
                     /locus_tag="IWO50_000370"
     CDS             56176..56358
                     /locus_tag="IWO50_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0357520.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            56358..56771
                     /locus_tag="IWO50_000371"
     CDS             56358..56771
                     /locus_tag="IWO50_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0357521.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            56894..58075
                     /locus_tag="IWO50_000372"
     CDS             56894..58075
                     /locus_tag="IWO50_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0357522.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        19182 a        10093 c        12794 g        16456 t
ORIGIN      
        1 cacagggcta ttccggttat gagtatgtac tataccatgt aaatcagcag catagcaaag
       61 cattttctcg cccggtaggt ttgcgacctg ccgggcgatt tttgtcgtct tttgcggccc
      121 ggctgtcccc aaaaaaattt tttgagaaat tttcaaaaag gaggtattta gcgggtagct
      181 ggcggccttg tcaaagtctt gttagcggaa agggagagaa ccacctaagt cccccacaga
      241 aagggggtga gaagatggat gctatccccc gcgactatga agcgcggtgc atgttcgatg
      301 ctttttgcaa gacggtactg cgtaacgagg ccaaaagcta cttggctgaa atgaagcgcc
      361 gccgtgaccg tgaagtgtca ctaagctctt tgtcacaggc agatttggac aagctctgta
      421 cagtagatca ctaccccagc gacagcttta cgttttcgtc gcatggctat gacctacata
      481 tcaacaatga gcttgttgcc gaagcctttg ccgcgctgcc cagcatggag cagagtattt
      541 taatcctgca ctgtgttctg gatatggcag atggtgagat cggcggcctt gtgggaatgt
      601 cccggagtgc cgttcagcgg cacaggacaa ataccttatc ggagctgcgg aaacagctaa
      661 aggcgttgat gccgaaggga ggttagacgc ggatgaagaa cccgaactac aagggcattg
      721 agcttttgcc cctttcggtg atcgaggccg cccgcgctgg ggatgccgga gccgtagagc
      781 gcgttttgca gtattacagc ggttacatga acaagctgtg tacccggacg ctctatgacg
      841 aaaacggcta tccgcatgtg tgtctggacg aggccatgaa gcgccgtctg gaaatcaagc
      901 tcatccacgc tatcgtcacg aactgacgaa ccggccccgg cagcatgaaa cggcttaccc
      961 tttccccgtt tcggtgtttt cccggtggcc tgatcgcacc ttgataaata tgcccgcaag
     1021 ataacggcgg cgcatcatag ggcatacgga cacattctgt ctgtgccgag ccggacggct
     1081 ggtgcgccat gatacttttc cctacgggaa aagcggagcg aggaacacca cgccgcaatg
     1141 tgagcgtggc ggcttgcggg cgacgacgta caggcaggac tatgatactc ccttatagcc
     1201 acagtccgag cgttaaaagc gtcgcaggca atgggtaggg ccgcgtgaga accacgcggg
     1261 ggtgaaagtc ccgtggagct gtgccagcag ccgtccgtct atgcccctaa atcacacaaa
     1321 cctttgaatt tgtgaaaggg ggctgcatta tgaaccatga tgtgcctatt tgggaaaaat
     1381 acacgctgac gattgaggaa gcgtctaaat atttccgtat cggagaaaac aaattgcgta
     1441 agctggccga ggaaaatccg gcctctggct gggtcattat gaatggcaac cgcatccaga
     1501 tcaaacgcaa gcagtttgag aaaataattg acactttgga cacaatctaa tagtgaaaaa
     1561 gggccgcaga tatgttataa taatttataa gcatatatgc ggctctttcc gtcatggaaa
     1621 ggagcatgac gagatgtcag aaaaaagacg agacagcaaa aatcgcgttt tgcggtctgg
     1681 agagagccag agaaaagacg gacgatatgc ttacaaatat gttgatacct ttggaaaacc
     1741 gcagtttgtt tactcatgga agttagtgcc tacggataag accccggcag gtaaacggga
     1801 ggatatttca ctccgggaaa aggagaaaga aatccagaaa gaccttgacg acgggataga
     1861 caccatcggc aagaaaatga ccgtctgcca gctctacgcc aagcagatca ggcaccgggg
     1921 caatgtgaag cacaacacca aactgggccg ggaacggctg atgcgtattc tggaacaaga
     1981 tcggctgggg agctgcccga ttgacagtgt gaagctgtcc gacgcaaagg aatgggcgct
     2041 tcggatgaaa gaaaagggcc tttcttataa gaccatcaat aacgacaagc gttccctgaa
     2101 agcggctttt tacaccgcca tacaggacga ttgcatccga aaaaacccgt ttgacttcca
     2161 gcttagtgac gttctggacg acgatacgga accaaaggtg cctcttacac cagcacagga
     2221 agaaagtttt ctgtccttta ttcaaggtga taaggtttac caaaagcatt atgacgcaat
     2281 cgttattctg ctggggacag ggcttcgcat ttcggaactg tgcggactga cggacaaaga
     2341 tttggatttt gaaaaccggg taatcatcgt gagccaccag cttttgagaa ataccggcgt
     2401 cggttactat attgacgaac ccaagaccca aagcggcgtc agaaaaatcc ccatgaatga
     2461 ggaagtgtat caggcgttcc agcgagtgat aaaaaaccgc aaaggtgcaa agccgttcat
     2521 tattgacggg tatgccaatt tcctgtttct caaacagaac ggctatccca tgacggcggt
     2581 agattatggc gggatgtttg gcaggcttgt gaagaagtac aacaagagcc atgaagaagc
     2641 gttgccgaaa acgacgacgc cccatgctat gcgtcataca ttctgcaccc ggttagccaa
     2701 tgcgggaatg aaccccaaag ccttgcagta cattatggga cattccaata tcaccatgac
     2761 gctgaactat tatgcacacg ccacattcga ttccgcaagg gcagaaatgg aacggctggc
     2821 agcctgacac aaggccggtt ttactactcc atttactact tttgaggggg aaatggtgag
     2881 aggtaataag aacatttgta agtatctaac aatatcaaaa atgccggaaa agcccggaaa
     2941 cacgggctat accgacattc aagaacttct aaaaagataa tctaaaatcc catataattt
     3001 ttcctcaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
     3061 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
     3121 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
     3181 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
     3241 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
     3301 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
     3361 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
     3421 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
     3481 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
     3541 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
     3601 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
     3661 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     3721 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     3781 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     3841 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     3901 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     3961 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     4021 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     4081 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     4141 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     4201 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     4261 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     4321 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     4381 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     4441 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     4501 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     4561 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     4621 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     4681 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     4741 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     4801 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     4861 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     4921 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     4981 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     5041 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     5101 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     5161 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     5221 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     5281 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     5341 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     5401 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     5461 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     5521 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     5581 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     5641 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     5701 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     5761 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     5821 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     5881 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     5941 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     6001 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     6061 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     6121 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     6181 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     6241 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     6301 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     6361 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     6421 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     6481 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     6541 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     6601 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     6661 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     6721 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     6781 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     6841 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     6901 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     6961 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     7021 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     7081 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     7141 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     7201 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     7261 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     7321 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     7381 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     7441 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     7501 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     7561 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     7621 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     7681 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     7741 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     7801 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     7861 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     7921 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     7981 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     8041 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     8101 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     8161 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     8221 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     8281 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     8341 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     8401 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     8461 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     8521 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     8581 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     8641 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     8701 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     8761 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     8821 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     8881 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     8941 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     9001 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     9061 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     9121 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     9181 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     9241 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     9301 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     9361 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     9421 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     9481 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     9541 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     9601 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     9661 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     9721 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     9781 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     9841 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     9901 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     9961 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
    10021 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
    10081 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
    10141 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
    10201 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
    10261 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
    10321 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
    10381 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
    10441 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
    10501 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
    10561 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
    10621 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
    10681 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
    10741 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
    10801 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
    10861 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
    10921 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
    10981 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
    11041 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
    11101 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
    11161 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
    11221 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
    11281 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
    11341 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
    11401 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
    11461 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
    11521 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
    11581 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
    11641 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
    11701 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
    11761 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
    11821 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
    11881 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
    11941 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
    12001 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
    12061 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
    12121 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
    12181 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
    12241 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
    12301 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
    12361 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
    12421 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
    12481 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
    12541 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
    12601 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
    12661 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    12721 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    12781 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    12841 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    12901 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    12961 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    13021 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    13081 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    13141 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    13201 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    13261 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    13321 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    13381 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    13441 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    13501 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    13561 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    13621 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    13681 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    13741 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    13801 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    13861 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    13921 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    13981 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    14041 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    14101 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    14161 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    14221 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    14281 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    14341 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    14401 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    14461 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    14521 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    14581 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    14641 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    14701 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    14761 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    14821 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    14881 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    14941 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    15001 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    15061 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    15121 ttagctgccc ttgtacaaag atgggtatca atcaaattct caccagttgt atcaactgta
    15181 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    15241 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    15301 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    15361 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    15421 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    15481 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    15541 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    15601 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    15661 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    15721 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    15781 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    15841 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    15901 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    15961 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    16021 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    16081 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    16141 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    16201 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    16261 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    16321 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    16381 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    16441 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    16501 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    16561 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    16621 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    16681 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    16741 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    16801 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    16861 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    16921 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    16981 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    17041 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    17101 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    17161 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    17221 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    17281 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    17341 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    17401 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    17461 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    17521 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    17581 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    17641 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    17701 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    17761 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    17821 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    17881 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    17941 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    18001 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    18061 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    18121 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    18181 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    18241 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    18301 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    18361 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    18421 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    18481 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    18541 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    18601 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    18661 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    18721 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    18781 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    18841 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    18901 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    18961 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    19021 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    19081 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    19141 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    19201 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    19261 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    19321 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    19381 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    19441 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    19501 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    19561 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    19621 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    19681 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    19741 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    19801 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    19861 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    19921 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    19981 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    20041 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    20101 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    20161 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    20221 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    20281 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    20341 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    20401 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    20461 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    20521 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    20581 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    20641 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    20701 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    20761 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    20821 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    20881 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    20941 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    21001 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    21061 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    21121 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    21181 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    21241 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    21301 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    21361 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    21421 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    21481 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    21541 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    21601 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    21661 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    21721 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    21781 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    21841 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    21901 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    21961 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    22021 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    22081 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    22141 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    22201 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    22261 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    22321 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    22381 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    22441 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    22501 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    22561 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    22621 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    22681 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    22741 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    22801 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    22861 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    22921 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    22981 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    23041 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    23101 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    23161 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    23221 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    23281 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    23341 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    23401 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    23461 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    23521 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    23581 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    23641 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    23701 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    23761 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    23821 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    23881 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    23941 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    24001 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    24061 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    24121 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    24181 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    24241 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    24301 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    24361 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    24421 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    24481 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    24541 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    24601 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    24661 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    24721 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    24781 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    24841 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    24901 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    24961 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    25021 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    25081 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    25141 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    25201 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    25261 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    25321 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    25381 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    25441 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    25501 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    25561 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    25621 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    25681 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    25741 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    25801 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    25861 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    25921 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    25981 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    26041 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    26101 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    26161 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    26221 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    26281 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    26341 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    26401 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    26461 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    26521 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    26581 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    26641 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    26701 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    26761 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    26821 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    26881 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    26941 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    27001 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    27061 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    27121 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    27181 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    27241 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    27301 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    27361 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    27421 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    27481 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    27541 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    27601 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    27661 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    27721 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    27781 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    27841 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    27901 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    27961 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    28021 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    28081 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    28141 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    28201 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    28261 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    28321 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    28381 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    28441 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    28501 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    28561 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    28621 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    28681 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    28741 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    28801 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    28861 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    28921 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    28981 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    29041 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    29101 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    29161 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    29221 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    29281 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    29341 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    29401 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    29461 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    29521 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    29581 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    29641 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    29701 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    29761 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    29821 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    29881 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    29941 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    30001 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    30061 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    30121 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    30181 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    30241 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    30301 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    30361 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    30421 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    30481 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    30541 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    30601 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    30661 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    30721 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    30781 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    30841 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    30901 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    30961 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    31021 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    31081 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    31141 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    31201 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    31261 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    31321 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    31381 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    31441 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    31501 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    31561 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    31621 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    31681 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    31741 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    31801 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    31861 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    31921 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    31981 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    32041 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    32101 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    32161 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    32221 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    32281 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    32341 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    32401 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    32461 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    32521 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    32581 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    32641 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    32701 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    32761 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    32821 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    32881 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    32941 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    33001 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    33061 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    33121 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    33181 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    33241 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    33301 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    33361 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    33421 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    33481 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    33541 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    33601 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    33661 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    33721 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    33781 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    33841 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    33901 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    33961 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    34021 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    34081 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    34141 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    34201 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    34261 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    34321 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    34381 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    34441 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    34501 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    34561 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    34621 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    34681 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    34741 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    34801 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    34861 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    34921 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    34981 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    35041 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    35101 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    35161 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    35221 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    35281 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    35341 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    35401 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    35461 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    35521 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    35581 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    35641 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    35701 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    35761 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    35821 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    35881 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    35941 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    36001 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    36061 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    36121 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    36181 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    36241 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    36301 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    36361 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    36421 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    36481 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    36541 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    36601 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    36661 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    36721 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    36781 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    36841 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    36901 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    36961 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    37021 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    37081 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    37141 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    37201 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    37261 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    37321 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    37381 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    37441 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    37501 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    37561 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    37621 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    37681 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    37741 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    37801 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    37861 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    37921 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    37981 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    38041 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    38101 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    38161 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    38221 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    38281 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    38341 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    38401 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    38461 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    38521 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    38581 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    38641 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    38701 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    38761 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    38821 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    38881 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    38941 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    39001 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    39061 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    39121 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    39181 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    39241 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    39301 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    39361 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    39421 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    39481 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    39541 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    39601 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    39661 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    39721 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    39781 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    39841 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    39901 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    39961 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    40021 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    40081 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    40141 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    40201 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    40261 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    40321 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    40381 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    40441 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    40501 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    40561 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    40621 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    40681 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    40741 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    40801 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    40861 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    40921 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    40981 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    41041 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    41101 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    41161 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    41221 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    41281 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    41341 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    41401 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    41461 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    41521 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    41581 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    41641 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    41701 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    41761 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    41821 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    41881 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    41941 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    42001 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    42061 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    42121 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    42181 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    42241 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    42301 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    42361 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    42421 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    42481 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    42541 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    42601 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    42661 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    42721 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    42781 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    42841 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    42901 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    42961 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    43021 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    43081 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    43141 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    43201 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    43261 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    43321 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    43381 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    43441 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    43501 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    43561 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    43621 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    43681 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    43741 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    43801 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    43861 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    43921 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    43981 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    44041 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    44101 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    44161 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    44221 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    44281 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    44341 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    44401 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    44461 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    44521 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    44581 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    44641 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    44701 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    44761 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    44821 accagttatc tggttgccag atccattcga atacgtcttt ttcaagtagt tcgaaagctg
    44881 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    44941 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    45001 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    45061 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    45121 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    45181 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    45241 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    45301 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    45361 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    45421 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    45481 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    45541 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    45601 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    45661 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    45721 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    45781 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    45841 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    45901 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    45961 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    46021 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    46081 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    46141 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    46201 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    46261 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    46321 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    46381 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    46441 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    46501 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    46561 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    46621 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    46681 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    46741 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    46801 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    46861 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    46921 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    46981 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    47041 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    47101 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    47161 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    47221 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    47281 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    47341 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    47401 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    47461 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    47521 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    47581 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    47641 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    47701 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    47761 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    47821 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    47881 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    47941 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    48001 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    48061 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    48121 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    48181 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    48241 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    48301 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    48361 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    48421 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    48481 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    48541 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    48601 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    48661 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    48721 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    48781 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    48841 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    48901 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    48961 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    49021 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    49081 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    49141 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    49201 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    49261 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    49321 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    49381 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    49441 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    49501 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    49561 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    49621 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    49681 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    49741 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    49801 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    49861 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    49921 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    49981 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    50041 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    50101 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    50161 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    50221 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    50281 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    50341 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    50401 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    50461 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    50521 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    50581 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    50641 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    50701 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    50761 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    50821 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    50881 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    50941 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    51001 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    51061 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    51121 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    51181 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    51241 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    51301 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    51361 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    51421 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    51481 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    51541 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    51601 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    51661 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    51721 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    51781 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    51841 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    51901 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    51961 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    52021 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    52081 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    52141 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    52201 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    52261 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    52321 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    52381 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    52441 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    52501 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    52561 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    52621 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    52681 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    52741 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    52801 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    52861 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    52921 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    52981 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    53041 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    53101 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    53161 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    53221 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    53281 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    53341 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    53401 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    53461 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    53521 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    53581 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    53641 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    53701 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    53761 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    53821 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    53881 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    53941 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    54001 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    54061 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    54121 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    54181 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    54241 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    54301 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    54361 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    54421 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    54481 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    54541 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    54601 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    54661 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    54721 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    54781 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    54841 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    54901 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    54961 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    55021 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    55081 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    55141 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    55201 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    55261 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    55321 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    55381 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    55441 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    55501 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    55561 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    55621 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    55681 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    55741 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    55801 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    55861 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    55921 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    55981 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    56041 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    56101 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    56161 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    56221 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    56281 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    56341 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    56401 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    56461 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    56521 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    56581 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    56641 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    56701 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    56761 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    56821 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    56881 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    56941 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    57001 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    57061 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    57121 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    57181 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    57241 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    57301 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    57361 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    57421 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    57481 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    57541 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    57601 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    57661 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    57721 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    57781 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    57841 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    57901 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    57961 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    58021 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    58081 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    58141 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    58201 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    58261 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    58321 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    58381 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    58441 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    58501 aagcgatagg tcgctggttc aattc
//
LOCUS       DACEDM010000006        56349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEDM010000006 DACEDM010000000
VERSION     DACEDM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.006"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            290..1135
                     /locus_tag="IWO50_000373"
     CDS             290..1135
                     /locus_tag="IWO50_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0357523.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IWO50_000374"
     CDS             1416..2636
                     /locus_tag="IWO50_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0357524.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IWO50_000375"
     CDS             2633..5353
                     /locus_tag="IWO50_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0357525.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IWO50_000376"
     CDS             5337..6281
                     /locus_tag="IWO50_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0357526.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IWO50_000377"
     CDS             complement(6350..7360)
                     /locus_tag="IWO50_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0357527.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IWO50_000378"
     CDS             complement(7540..7884)
                     /locus_tag="IWO50_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0357528.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IWO50_000379"
     CDS             complement(7877..8311)
                     /locus_tag="IWO50_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0357529.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IWO50_000380"
     CDS             8449..9186
                     /locus_tag="IWO50_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357530.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IWO50_000381"
     CDS             9179..10390
                     /locus_tag="IWO50_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0357531.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IWO50_000382"
     CDS             10597..11376
                     /locus_tag="IWO50_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0357532.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IWO50_000383"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IWO50_000383"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0357533.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IWO50_000384"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IWO50_000384"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0357534.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IWO50_000385"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IWO50_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0357535.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IWO50_000386"
     CDS             14978..16027
                     /locus_tag="IWO50_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0357536.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IWO50_000387"
     CDS             16024..16302
                     /locus_tag="IWO50_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0357537.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IWO50_000388"
     CDS             16277..16747
                     /locus_tag="IWO50_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0357538.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IWO50_000389"
     CDS             16764..17066
                     /locus_tag="IWO50_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357539.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IWO50_000390"
     CDS             17083..17454
                     /locus_tag="IWO50_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0357540.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IWO50_000391"
     CDS             17426..17785
                     /locus_tag="IWO50_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357541.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IWO50_000392"
     CDS             17957..18964
                     /locus_tag="IWO50_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0357542.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IWO50_000393"
     CDS             18989..20173
                     /locus_tag="IWO50_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0357543.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IWO50_000394"
     CDS             complement(20610..22193)
                     /locus_tag="IWO50_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0357544.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IWO50_000395"
     CDS             complement(22771..22914)
                     /locus_tag="IWO50_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0357545.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="IWO50_000396"
     CDS             complement(23137..23616)
                     /locus_tag="IWO50_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0357546.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="IWO50_000397"
     CDS             complement(23689..23973)
                     /locus_tag="IWO50_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357547.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="IWO50_000398"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWO50_000398"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="IWO50_000399"
     CDS             complement(24317..25603)
                     /locus_tag="IWO50_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0357548.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="IWO50_000400"
     CDS             25741..26412
                     /locus_tag="IWO50_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0357549.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="IWO50_000401"
     CDS             26378..26866
                     /locus_tag="IWO50_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0357550.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="IWO50_000402"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="IWO50_000402"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0357551.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="IWO50_000403"
     CDS             27827..28591
                     /locus_tag="IWO50_000403"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0357552.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="IWO50_000404"
     CDS             28824..31037
                     /locus_tag="IWO50_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0357553.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="IWO50_000405"
     CDS             31048..31494
                     /locus_tag="IWO50_000405"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0357554.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="IWO50_000406"
     CDS             31665..32924
                     /locus_tag="IWO50_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0357555.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="IWO50_000407"
     CDS             complement(33013..33960)
                     /locus_tag="IWO50_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0357556.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="IWO50_000408"
     CDS             34354..35646
                     /locus_tag="IWO50_000408"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0357557.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="IWO50_000409"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="IWO50_000409"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0357558.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="IWO50_000410"
     CDS             37539..38414
                     /locus_tag="IWO50_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0357559.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="IWO50_000411"
     CDS             38680..40563
                     /locus_tag="IWO50_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0357560.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="IWO50_000412"
     CDS             complement(40638..40922)
                     /locus_tag="IWO50_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0357561.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="IWO50_000413"
     CDS             complement(40912..41646)
                     /locus_tag="IWO50_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0357562.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="IWO50_000414"
     CDS             41775..42398
                     /locus_tag="IWO50_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0357563.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="IWO50_000415"
     CDS             42590..43711
                     /locus_tag="IWO50_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0357564.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="IWO50_000416"
     CDS             43718..46846
                     /locus_tag="IWO50_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0357565.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="IWO50_000417"
     CDS             46865..50059
                     /locus_tag="IWO50_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0357566.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="IWO50_000418"
     CDS             complement(50205..50912)
                     /locus_tag="IWO50_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0357567.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51677..52075
                     /gene="spxA"
                     /locus_tag="IWO50_000419"
     CDS             51677..52075
                     /gene="spxA"
                     /locus_tag="IWO50_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0357568.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52407..53060
                     /locus_tag="IWO50_000420"
     CDS             52407..53060
                     /locus_tag="IWO50_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0357569.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53286..54284
                     /locus_tag="IWO50_000421"
     CDS             53286..54284
                     /locus_tag="IWO50_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0357570.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54460..56268
                     /gene="pepF"
                     /locus_tag="IWO50_000422"
     CDS             54460..56268
                     /gene="pepF"
                     /locus_tag="IWO50_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0357571.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18539 a         9724 c        11679 g        16407 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
    51421 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
    51481 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
    51541 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
    51601 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
    51661 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
    51721 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
    51781 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
    51841 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
    51901 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
    51961 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
    52021 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
    52081 taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt tttgttttta
    52141 caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat tgcacgaaaa
    52201 atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta
    52261 tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa attcgcttaa
    52321 cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc tatattaaac
    52381 attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa aatacgatcc
    52441 gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg gatttacttg
    52501 gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt gatatcgaag
    52561 aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga gacggattag
    52621 aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa gattctactg
    52681 ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc gatccggagg
    52741 aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt gcttttgaag
    52801 attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct tggactgatt
    52861 tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa aatctcaacc
    52921 aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag agcgatcgta
    52981 caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct atcgagcgaa
    53041 cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg
    53101 cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca
    53161 aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat gcagaatagc
    53221 tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag ttcagaggga
    53281 gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa catgcagaaa
    53341 aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga
    53401 aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt agtgaaagag
    53461 aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga cagtcagtag
    53521 aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat attttgttgg
    53581 cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt attgaaagaa
    53641 cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat tcttttcttg
    53701 gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga aaaagaggag
    53761 tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat catattacag
    53821 agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct agtagggacc
    53881 ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag
    53941 tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa aagaatacag
    54001 tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta tcatcgtgga
    54061 tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt tatcgaatgt
    54121 tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg ctttgccaaa
    54181 taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg gtctatcagc
    54241 agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta taaaaatagg
    54301 ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga tggaagcaga
    54361 cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact atgatctatt
    54421 ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa gcagttgcca
    54481 aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt ttcaagcgac
    54541 caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc tgaaaaacac
    54601 aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt cgtattgagg
    54661 gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga tcaagacact
    54721 ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc gaaagttagt
    54781 gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca gatttggcaa
    54841 tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca aatagtagat
    54901 aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc aggtgaaatc
    54961 tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt ttttccaacg
    55021 attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta
    55081 gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa
    55141 caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca taattttaaa
    55201 gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa tcatattcct
    55261 gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt gttacatcga
    55321 tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta tgatctttat
    55381 acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa agaaaaagct
    55441 ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa agcattctct
    55501 gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga
    55561 agctatgaca caaatccata tattttattg aattggcatg atacgctgga tcagctattt
    55621 acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc gaaccagcct
    55681 tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac gaatgaaaat
    55741 atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc
    55801 aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt cgctgaattc
    55861 gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga atacctaagt
    55921 gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga agacccagaa
    55981 attaaattcg aatggtcacg gataccgcat ttttattata attactatgt tttccaatac
    56041 tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca ggaaccagag
    56101 gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta
    56161 atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc aatgtcgatg
    56221 tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg ctttctattt
    56281 ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt
    56341 attttttta
//
LOCUS       DACEDM010000007        51758 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEDM010000007 DACEDM010000000
VERSION     DACEDM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.007"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            125..1168
                     /locus_tag="IWO50_000423"
     CDS             125..1168
                     /locus_tag="IWO50_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0357572.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1230..1694)
                     /locus_tag="IWO50_000424"
     CDS             complement(1230..1694)
                     /locus_tag="IWO50_000424"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0357573.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            1940..2887
                     /locus_tag="IWO50_000425"
     CDS             1940..2887
                     /locus_tag="IWO50_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0357574.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(3098..5170)
                     /locus_tag="IWO50_000426"
     CDS             complement(3098..5170)
                     /locus_tag="IWO50_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0357575.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(5103..5480)
                     /locus_tag="IWO50_000427"
     CDS             complement(5103..5480)
                     /locus_tag="IWO50_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0357576.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(5609..6169)
                     /locus_tag="IWO50_000428"
     CDS             complement(5609..6169)
                     /locus_tag="IWO50_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0357577.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            6319..7611
                     /locus_tag="IWO50_000429"
     CDS             6319..7611
                     /locus_tag="IWO50_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0357578.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(7671..8789)
                     /locus_tag="IWO50_000430"
     CDS             complement(7671..8789)
                     /locus_tag="IWO50_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0357579.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="IWO50_000431"
     CDS             complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="IWO50_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0357580.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="IWO50_000432"
     CDS             complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="IWO50_000432"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0357581.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            11995..12594
                     /locus_tag="IWO50_000433"
     CDS             11995..12594
                     /locus_tag="IWO50_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0357582.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="IWO50_000434"
     CDS             complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="IWO50_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0357583.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(14289..14633)
                     /locus_tag="IWO50_000435"
     CDS             complement(14289..14633)
                     /locus_tag="IWO50_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0357584.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(14770..15906)
                     /locus_tag="IWO50_000436"
     CDS             complement(14770..15906)
                     /locus_tag="IWO50_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0357585.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(16095..17069)
                     /locus_tag="IWO50_000437"
     CDS             complement(16095..17069)
                     /locus_tag="IWO50_000437"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0357586.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(17149..18024)
                     /locus_tag="IWO50_000438"
     CDS             complement(17149..18024)
                     /locus_tag="IWO50_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0357587.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            18154..20061
                     /locus_tag="IWO50_000439"
     CDS             18154..20061
                     /locus_tag="IWO50_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0357588.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(20602..23880)
                     /locus_tag="IWO50_000440"
     CDS             complement(20602..23880)
                     /locus_tag="IWO50_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0357589.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(24151..24696)
                     /locus_tag="IWO50_000441"
     CDS             complement(24151..24696)
                     /locus_tag="IWO50_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0357590.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(24708..25400)
                     /locus_tag="IWO50_000442"
     CDS             complement(24708..25400)
                     /locus_tag="IWO50_000442"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0357591.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(25419..25703)
                     /locus_tag="IWO50_000443"
     CDS             complement(25419..25703)
                     /locus_tag="IWO50_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357592.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(25716..26279)
                     /locus_tag="IWO50_000444"
     CDS             complement(25716..26279)
                     /locus_tag="IWO50_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0357593.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            26507..27169
                     /locus_tag="IWO50_000445"
     CDS             26507..27169
                     /locus_tag="IWO50_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0357594.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            27293..28489
                     /locus_tag="IWO50_000446"
     CDS             27293..28489
                     /locus_tag="IWO50_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0357595.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(28590..29966)
                     /locus_tag="IWO50_000447"
     CDS             complement(28590..29966)
                     /locus_tag="IWO50_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0357596.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLV
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(30003..30869)
                     /locus_tag="IWO50_000448"
     CDS             complement(30003..30869)
                     /locus_tag="IWO50_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0357597.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(30958..31983)
                     /locus_tag="IWO50_000449"
     CDS             complement(30958..31983)
                     /locus_tag="IWO50_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0357598.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(32001..32675)
                     /locus_tag="IWO50_000450"
     CDS             complement(32001..32675)
                     /locus_tag="IWO50_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0357599.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(32859..33752)
                     /locus_tag="IWO50_000451"
     CDS             complement(32859..33752)
                     /locus_tag="IWO50_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0357600.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(33826..35523)
                     /locus_tag="IWO50_000452"
     CDS             complement(33826..35523)
                     /locus_tag="IWO50_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0357601.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(35516..36829)
                     /locus_tag="IWO50_000453"
     CDS             complement(35516..36829)
                     /locus_tag="IWO50_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0357602.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(36829..40866)
                     /locus_tag="IWO50_000454"
     CDS             complement(36829..40866)
                     /locus_tag="IWO50_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0357603.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(41635..42153)
                     /locus_tag="IWO50_000455"
     CDS             complement(41635..42153)
                     /locus_tag="IWO50_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0357604.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(42155..42772)
                     /locus_tag="IWO50_000456"
     CDS             complement(42155..42772)
                     /locus_tag="IWO50_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0357605.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(42769..43623)
                     /locus_tag="IWO50_000457"
     CDS             complement(42769..43623)
                     /locus_tag="IWO50_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0357606.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(43620..44531)
                     /locus_tag="IWO50_000458"
     CDS             complement(43620..44531)
                     /locus_tag="IWO50_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0357607.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(44572..45906)
                     /locus_tag="IWO50_000459"
     CDS             complement(44572..45906)
                     /locus_tag="IWO50_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0357608.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            46114..46944
                     /locus_tag="IWO50_000460"
     CDS             46114..46944
                     /locus_tag="IWO50_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0357609.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(47188..47437)
                     /locus_tag="IWO50_000461"
                     /pseudo
     CDS             complement(47188..47437)
                     /locus_tag="IWO50_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(47628..47822)
                     /locus_tag="IWO50_000462"
     CDS             complement(47628..47822)
                     /locus_tag="IWO50_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357610.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(48327..48512)
                     /locus_tag="IWO50_000463"
     CDS             complement(48327..48512)
                     /locus_tag="IWO50_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357611.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(49362..49703)
                     /locus_tag="IWO50_000464"
     CDS             complement(49362..49703)
                     /locus_tag="IWO50_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0357612.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(49703..49933)
                     /locus_tag="IWO50_000465"
     CDS             complement(49703..49933)
                     /locus_tag="IWO50_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0357613.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            50220..50558
                     /locus_tag="IWO50_000466"
     CDS             50220..50558
                     /locus_tag="IWO50_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357614.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(50763..51338)
                     /locus_tag="IWO50_000467"
     CDS             complement(50763..51338)
                     /locus_tag="IWO50_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0357615.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT        15010 a        10965 c         9136 g        16647 t
ORIGIN      
        1 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
       61 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
      121 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
      181 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
      241 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
      301 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
      361 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
      421 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
      481 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
      541 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
      601 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
      661 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
      721 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
      781 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
      841 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
      901 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
      961 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
     1021 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
     1081 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
     1141 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
     1201 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
     1261 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
     1321 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
     1381 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
     1441 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
     1501 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
     1561 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
     1621 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
     1681 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
     1741 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
     1801 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
     1861 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
     1921 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
     1981 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
     2041 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
     2101 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
     2161 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
     2221 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     2281 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     2341 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     2401 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     2461 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     2521 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     2581 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     2641 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     2701 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     2761 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     2821 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     2881 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     2941 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     3001 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     3061 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
     3121 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
     3181 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
     3241 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
     3301 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
     3361 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
     3421 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
     3481 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
     3541 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
     3601 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
     3661 gcaatccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
     3721 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
     3781 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
     3841 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
     3901 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
     3961 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
     4021 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
     4081 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
     4141 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
     4201 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
     4261 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
     4321 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
     4381 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
     4441 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
     4501 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
     4561 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
     4621 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
     4681 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
     4741 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
     4801 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
     4861 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
     4921 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
     4981 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
     5041 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
     5101 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
     5161 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
     5221 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
     5281 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
     5341 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
     5401 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
     5461 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
     5521 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
     5581 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
     5641 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
     5701 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
     5761 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
     5821 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
     5881 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
     5941 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
     6001 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
     6061 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
     6121 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
     6181 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
     6241 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
     6301 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
     6361 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
     6421 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
     6481 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
     6541 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
     6601 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
     6661 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
     6721 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
     6781 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
     6841 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
     6901 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
     6961 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
     7021 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
     7081 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
     7141 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
     7201 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
     7261 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
     7321 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
     7381 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
     7441 tccagtgcat ttggttatcg tggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
     7501 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
     7561 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
     7621 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
     7681 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
     7741 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
     7801 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
     7861 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
     7921 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
     7981 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
     8041 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
     8101 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
     8161 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
     8221 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
     8281 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
     8341 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
     8401 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
     8461 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
     8521 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
     8581 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
     8641 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
     8701 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
     8761 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
     8821 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
     8881 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
     8941 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
     9001 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
     9061 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
     9121 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
     9181 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
     9241 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
     9301 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
     9361 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
     9421 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
     9481 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
     9541 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
     9601 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
     9661 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
     9721 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
     9781 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
     9841 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
     9901 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
     9961 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
    10021 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
    10081 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
    10141 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
    10201 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
    10261 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
    10321 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
    10381 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
    10441 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
    10501 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
    10561 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
    10621 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
    10681 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
    10741 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
    10801 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
    10861 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
    10921 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
    10981 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
    11041 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
    11101 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
    11161 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
    11221 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    11281 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    11341 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    11401 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    11461 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    11521 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    11581 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    11641 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    11701 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    11761 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    11821 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    11881 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    11941 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    12001 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    12061 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    12121 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    12181 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    12241 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    12301 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    12361 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    12421 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    12481 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    12541 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    12601 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    12661 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    12721 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    12781 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    12841 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    12901 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    12961 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    13021 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    13081 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    13141 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    13201 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    13261 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    13321 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    13381 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    13441 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    13501 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    13561 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    13621 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    13681 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    13741 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    13801 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    13861 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    13921 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    13981 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    14041 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    14101 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    14161 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    14221 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    14281 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    14341 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    14401 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    14461 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    14521 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    14581 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    14641 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    14701 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    14761 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    14821 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    14881 tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    14941 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    15001 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    15061 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    15121 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    15181 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    15241 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    15301 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    15361 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    15421 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    15481 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    15541 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    15601 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    15661 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    15721 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    15781 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    15841 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    15901 tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata aaatagcgaa
    15961 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    16021 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctttc
    16081 cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg actcatcggt
    16141 gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt aacgattgtt
    16201 aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca aatatctttg
    16261 attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg tgacgcacag
    16321 gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc aagcgttgct
    16381 cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc accgatcaca
    16441 taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt gctgttcaaa
    16501 tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac aactacgatg
    16561 tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat cgtaaataaa
    16621 ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc taaagtcagt
    16681 actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt gatcggttca
    16741 tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac gacattgatc
    16801 gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat atagtaatca
    16861 ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat actttcttcg
    16921 atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc taattctgtt
    16981 ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact aaaaatcttc
    17041 aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct ctattcttcg
    17101 atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt aaggaagtga
    17161 aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc agttgtcgtg
    17221 tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg
    17281 attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt tgccttcacg
    17341 tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac gctcatgatt
    17401 gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag
    17461 tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt
    17521 tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat aataggcaag
    17581 gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc atttcttata
    17641 cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct ttcctgacct
    17701 tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag ataactcgat
    17761 tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat aagtatattt
    17821 tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac tttgatcttc
    17881 aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct gagtgctggc
    17941 taatttttta atttttttat ctactttgaa atataaaggt ttatccacta cttgattcag
    18001 ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa atcagtttga
    18061 ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt gtaagataat
    18121 gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt gaaacaagat
    18181 ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc agataaacca
    18241 gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat gggagaaaca
    18301 gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca
    18361 tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca accgcttcaa
    18421 cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa gcaatttcca
    18481 gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc agatatgaag
    18541 aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc agataagacg
    18601 tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt tgatatggct
    18661 gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa
    18721 gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga aaacgcagat
    18781 tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac agataaactg
    18841 gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt gatcgtaact
    18901 gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt tccgttattc
    18961 aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc agaagaattc
    19021 cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag
    19081 aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg cgattactac
    19141 ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg
    19201 atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc aacaaaagaa
    19261 aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga taagatccca
    19321 gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt gctttcaaat
    19381 actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa caaaccagtg
    19441 gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca tccatttcgg
    19501 aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct agaacaatca
    19561 agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc ccatgcattt
    19621 gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg gacagaagaa
    19681 gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga tggtctgcct
    19741 tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc tgatgcaggc
    19801 ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat cgaggaattc
    19861 tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca acaactttta
    19921 ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt
    19981 gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct agcgccagaa
    20041 aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa
    20101 ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt ttcgaagaag
    20161 ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt caagtagtaa
    20221 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ctaccgagaa
    20281 actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt
    20341 agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    20401 ggcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct gcatcaagta
    20461 ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga tagaaggaag
    20521 tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa ttgttttaca
    20581 tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa caactacacc
    20641 gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc ccgtttttgg
    20701 gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat gagaaggttt
    20761 tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg gtttgtcatt
    20821 tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg taacagattc
    20881 tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga cttctagcgt
    20941 atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt gagcacttga
    21001 ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa agacaggcgt
    21061 tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt
    21121 tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat tattttgttc
    21181 atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt ccgaagacgg
    21241 tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca ctctttcctt
    21301 ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga tacttccatt
    21361 ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag actcgatcca
    21421 atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt cattccaata
    21481 gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga tctgctctct
    21541 catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt ctgtgtttcc
    21601 tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg tactgctatt
    21661 gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt cgaaagacaa
    21721 actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga atccattacc
    21781 gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg agtttttgat
    21841 gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc
    21901 attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca gtttggccgc
    21961 aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac agttcttgac
    22021 taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga tttggattcc
    22081 tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc cagaaatctg
    22141 taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac cccagtagtg
    22201 actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac gcttgttttc
    22261 tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa cgccttttgg
    22321 aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg tctgcccagc
    22381 atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc cgtttcctgc
    22441 atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt agtccactgt
    22501 atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg gcggatagtt
    22561 tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt ttgtctgata
    22621 atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca cctctcctgt
    22681 tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca tagtatgatc
    22741 ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca agcgagtagg
    22801 tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga ttccttctac
    22861 cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt catctgaagc
    22921 gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg tttcaggatc
    22981 gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca gtttagtaat
    23041 cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat aagaacctgt
    23101 ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg agacactccc
    23161 aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc gtgtccccaa
    23221 tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc tatccgagtc
    23281 ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc
    23341 aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt cttgggcatt
    23401 cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg
    23461 gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt ttccgccgtt
    23521 attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc tactagcact
    23581 tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag attgcttttt
    23641 tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg tatccatatc
    23701 ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca cgtgtgcatt
    23761 ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa ttaaaaccgg
    23821 ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt tttttaacaa
    23881 gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct aatttatttt
    23941 tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa aaatataact
    24001 taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt ttaaaataac
    24061 gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta ttattttgtg
    24121 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
    24181 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg catctttgaa
    24241 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
    24301 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
    24361 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
    24421 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
    24481 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
    24541 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
    24601 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
    24661 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
    24721 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
    24781 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
    24841 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
    24901 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
    24961 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
    25021 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
    25081 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
    25141 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
    25201 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
    25261 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
    25321 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
    25381 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
    25441 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
    25501 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
    25561 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
    25621 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
    25681 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
    25741 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
    25801 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
    25861 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
    25921 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
    25981 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
    26041 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
    26101 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
    26161 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
    26221 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
    26281 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
    26341 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
    26401 tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa
    26461 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
    26521 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
    26581 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
    26641 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
    26701 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
    26761 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
    26821 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
    26881 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
    26941 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
    27001 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
    27061 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
    27121 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
    27181 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
    27241 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
    27301 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
    27361 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
    27421 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
    27481 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
    27541 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
    27601 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
    27661 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
    27721 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
    27781 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
    27841 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
    27901 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
    27961 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
    28021 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
    28081 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
    28141 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
    28201 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
    28261 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
    28321 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
    28381 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
    28441 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
    28501 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
    28561 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
    28621 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
    28681 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
    28741 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
    28801 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
    28861 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
    28921 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
    28981 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
    29041 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
    29101 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
    29161 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
    29221 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
    29281 cccaagaaag caaaaaacac aagagctgca ccaactagca caccggaaaa accaaaaggc
    29341 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
    29401 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
    29461 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
    29521 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
    29581 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
    29641 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
    29701 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
    29761 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
    29821 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
    29881 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
    29941 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
    30001 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
    30061 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
    30121 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
    30181 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
    30241 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
    30301 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
    30361 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
    30421 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
    30481 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
    30541 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
    30601 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
    30661 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
    30721 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
    30781 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
    30841 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
    30901 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
    30961 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
    31021 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
    31081 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
    31141 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
    31201 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
    31261 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
    31321 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
    31381 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
    31441 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
    31501 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
    31561 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
    31621 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
    31681 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
    31741 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
    31801 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
    31861 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
    31921 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
    31981 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
    32041 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
    32101 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
    32161 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
    32221 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
    32281 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
    32341 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
    32401 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
    32461 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
    32521 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
    32581 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
    32641 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
    32701 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
    32761 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
    32821 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
    32881 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
    32941 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
    33001 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
    33061 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
    33121 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
    33181 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
    33241 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
    33301 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
    33361 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
    33421 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
    33481 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
    33541 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
    33601 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
    33661 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
    33721 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
    33781 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
    33841 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
    33901 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
    33961 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
    34021 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
    34081 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    34141 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    34201 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    34261 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    34321 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    34381 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    34441 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    34501 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    34561 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    34621 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    34681 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    34741 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    34801 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    34861 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    34921 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    34981 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    35041 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    35101 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    35161 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    35221 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    35281 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    35341 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    35401 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    35461 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    35521 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    35581 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    35641 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    35701 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    35761 ctaattcatc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    35821 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    35881 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    35941 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    36001 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    36061 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    36121 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    36181 catttttagg atagatatga atctcttgca gttcctcgct tgattctgtc gggtgataaa
    36241 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    36301 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    36361 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    36421 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
    36481 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    36541 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    36601 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    36661 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    36721 ccacgacatc ttcccctgat tcttctgccg acatcgcctg tggaagaagg actgctgcaa
    36781 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    36841 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    36901 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    36961 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    37021 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    37081 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    37141 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    37201 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    37261 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    37321 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    37381 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    37441 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    37501 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    37561 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    37621 taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa
    37681 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    37741 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    37801 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    37861 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    37921 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    37981 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    38041 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    38101 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    38161 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    38221 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    38281 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    38341 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    38401 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    38461 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    38521 ggctgcatat tttttgattt ggaattgata cgtctcatat actaactgat aacgtggtgg
    38581 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
    38641 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    38701 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    38761 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    38821 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    38881 ctgaatatag gccatattgt tcactgtgta aaaatttccg tcttgagctc ccgcatcaag
    38941 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    39001 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    39061 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    39121 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    39181 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    39241 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    39301 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    39361 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    39421 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    39481 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    39541 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    39601 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    39661 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    39721 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    39781 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    39841 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    39901 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    39961 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    40021 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    40081 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    40141 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    40201 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    40261 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    40321 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    40381 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    40441 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    40501 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    40561 ccaatttcct aacacttttg tatagtcatt cgttgtaaat ttcgggtagc gatgctgttc
    40621 gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag ctgttggccc
    40681 aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg cttgaccgct
    40741 gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt gaatttcagc
    40801 ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc tttcatcaac
    40861 caccacttga agaggtagtt ccccttcgtt cacatcgggg atcttttctg tttcaaaaat
    40921 caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt cttgaccatc
    40981 gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac ctgttccacg
    41041 aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac gtattctacc
    41101 ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt cttctttttc
    41161 aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat cctcctttgt
    41221 ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact ggaaatatgg
    41281 tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc ctttcacatc
    41341 cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa caatgcccat
    41401 ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt tagttaaaaa
    41461 acaccaatta tacaacattt attattaaaa ttaaattata aaattttata acgttctcat
    41521 ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc tcgaaacaaa
    41581 aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat taattcagat
    41641 aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta aaatgtcagt
    41701 aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc
    41761 taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa aggctagcgc
    41821 tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga caagccgacg
    41881 atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa
    41941 aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat caggtatcaa
    42001 gactaaaaca gcagcaagga gtaaccaaat aaaaccacca ttagagatat tactgataaa
    42061 tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact tgttgataat
    42121 actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta agaacctttt
    42181 cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg aaacctaata
    42241 aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca
    42301 aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta atccaagaag
    42361 cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg cttaatagaa
    42421 gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa tagcggaaaa
    42481 tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct tgctttccat
    42541 aaagagagaa gttctcacga aactttttcc agaatagtga accgagcttt ttctcttcat
    42601 tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct aatgtgaaaa
    42661 caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga atccattcaa
    42721 tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc atccttttga
    42781 acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat
    42841 cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg tcgttgcttg
    42901 gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt ggatatataa
    42961 cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa
    43021 ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat tcgctccgtc
    43081 gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac gcatcagaaa
    43141 tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag tacctaccca
    43201 gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg ggatcatcat
    43261 cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct tcgcaaatcc
    43321 ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag tgatgaataa
    43381 tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat agttttccca
    43441 tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca ttggtttcaa
    43501 tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta ctgtcatgag
    43561 gacataactt agtatttttt ttgtttgccg attcacactg gtctttttga aataatccat
    43621 catttgccgt cacctccaga ataatagacc cacttaggtg caagcttcaa attgacaaac
    43681 gtcagaacca acacgatcag aaacaggatc attgacatag ccatcgcata acccatatca
    43741 aaagctacga acgccttgtt ccacatatgc agattataaa aaagcaaaga atttgcaggt
    43801 ccgcctgttc catttttcgt catgacataa gcttcttgaa aaatttggaa agaaccgatc
    43861 gtagaggtga tcacatcgta aaaaataata ggagtgatta atggcaaagt aatacttttg
    43921 aactttcccc aaacgcctgc tccatcgatt tctgctgatt catataaaga tctaggaaca
    43981 ttttgtaatg tagctaagta aagtagcatt gctcctccag aactccatat tttcatcaaa
    44041 atcaacgcag gtttcgtcca ttgtggatca aacagccaag ctggtccagg gatatggaac
    44101 cagcccagaa atgtgttgat caatcctgca gatggagaaa gcagctgcat ccataagatg
    44161 tagactgcta ctcctgataa gactgccggt aaatagaaag ctgtacggaa tacactgatt
    44221 cctttgattt tttgattgag tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt
    44281 acattgaaga tgacgaaata aacggtgttg tataacgatt tccaaaataa atcatcgtga
    44341 acgaacattc ttttgaaatt tgctagacca atgaaattca tcttggatgt caaattataa
    44401 tcggtgaaac taccaataac tgaaaaaagc ataggcccta gcataaatac caagaacccg
    44461 ataataaaag gcgagataaa taaataggcc catcttgctt cttttttctt tcggatagat
    44521 aaacgcttca aattttcttg cctcctctgt acctagacag tcaaaaatgg attattgatt
    44581 ttgcttgact aagttttcta ctgctttttg tgcatctgcc aatccttctt ttggtgattt
    44641 tgttcctaaa agaatctggt caatctgttc attcactaat gtcgtgtaat ccggagcatc
    44701 taaaggaact ggtgtgatca ccgtattttt aaagttttcg tccgccattt gatagatcat
    44761 ttttccttta tccgttaggt tgtcattatt cactaaattc tcattggctg tcttgctcgc
    44821 catgatatca aaactttttt cgccgaattt ttcttgtact tctggagaag aaagatattt
    44881 gatgaattca taagaggcct caggatcttt tgctccgtat ggtacttcca agacgaatcc
    44941 gccaccccac gaccaatggc ctgaaccttc ttctttttct gggagctgca tcacgccaaa
    45001 atcaaaatca tccggtgcat tttccatcaa actagagtaa taattgatat tttgggcacg
    45061 catggctact aatccggaaa taaatggatc agatacacca gacccaaatt cggcttctaa
    45121 tttattgatc gtatttttcc cataataatc ttgccagtct tggatccact ccaaggcttc
    45181 cactttattt ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt
    45241 atctgcgttt agcgcccata catcagcccc taaattccat aatggataaa aaccgatgcg
    45301 tgtaaattct tttccttctt ttttatctag tttacgtgct gtttcttcta attcatccca
    45361 agttgtcggt agatcttgtt cactgattcc agcttcttta aataactttt tattataaaa
    45421 aatgacttgt gtatatgtat tgaatgggat cgcataagga tcaccttcat attctactgt
    45481 atcccaaagc tgcggataaa aatccttaga agtgtctttt tctacatatt tggagagatt
    45541 agttgcttgc tgtgcttctg ctctttgggc aacggaattt atgtcctgta caataacatc
    45601 gggtgcattt ccagctgtta ttgcagataa agatttagtc cagatgtcac cccatggctg
    45661 atacttataa tttacatgga tctcgttttg tgattgattg aaatcctcaa tgatttcttc
    45721 gatagcttct tttcgtgcac cggatcccca aaaggaccag aaatcaatat tttttcttcc
    45781 atcacttgtt ttttcagaag aatttgctgc acttgaactg gcatcgcttg cattacttga
    45841 tccgcatcct cctaataaaa acaatgctga acttgccagc aaccctgtaa acaataattt
    45901 tttcatattt tacctccaaa ttttaatgta accgatgtcg gttttaatat aacgatatca
    45961 ttttgttctg aaaataacaa tttataaggt atgattctaa aaaaattatt aatgatacca
    46021 atactttata taattcgcaa gcgttttctt tgaatttttt aatctttcgt agcagaatga
    46081 gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa agaattgcag
    46141 tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca ggagctgatc
    46201 ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg ctggagctgc
    46261 cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga aatcggccac
    46321 gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa
    46381 gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat gaatccacag
    46441 cacgcgatcg tagctggctt aggacctcgt cctgctgaca gcgtgggcaa tccatggagc
    46501 ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa tttaaatgcc
    46561 gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga tcgtggcgta
    46621 aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct atgggaatgt
    46681 gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt
    46741 gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg agaccaaagt
    46801 agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata tcaatggttg
    46861 aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc aaaaatgtat
    46921 gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt tttgttccac
    46981 acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca aaagggtggt
    47041 tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg gctttcatat
    47101 tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag gtttatgtac
    47161 tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat ctaacgcact
    47221 atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt gtcctacttt
    47281 tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt ttagatttgc
    47341 acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt atttttcttc
    47401 tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg gctaaactat
    47461 ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc tcggcgcgct
    47521 caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag aaacaacggg
    47581 ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta tttttttgat
    47641 tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata aaaaccagaa
    47701 cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact agccaacaat
    47761 atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag tgttgttttc
    47821 atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt actagaaaaa
    47881 ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc aggccatctt
    47941 gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat ttgtggcggt
    48001 gacctccaat ttttaactct tctatctccc atccagtata cataaccttg atacttcgac
    48061 cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct aacttagttg
    48121 ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa gtatctgaac
    48181 tattaatttt taaactatca tttttatcct caactacatg cacatctgtt gcaacttcac
    48241 tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct tcagctgacg
    48301 ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc aacagcaaaa
    48361 aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga aaaattcagc
    48421 aactcattac tgtatgattt tagcagccca accaacatat accctataaa agctaataag
    48481 atggttagga atcctttaga atttttactc attcatagac acttccttaa ataactttta
    48541 ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata aaaaaaacct
    48601 tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact gacataagat
    48661 cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta gagaataaac
    48721 taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac tttggttcac
    48781 tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa
    48841 ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga tgttccagtg
    48901 atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt tgtgttattc
    48961 agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta cgtttttgag
    49021 catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt tttaggacta
    49081 aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact tttcgtatac
    49141 gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa
    49201 atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata cggaaattaa
    49261 tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt aatttcgtta
    49321 tgtttagcat ataaaaatat tatagagttt acttacaatg cttaaaacat atactgtata
    49381 atctgatcaa ttttagccat gtcttgcaat ggtaagtttt ctattttttt gagttttcgt
    49441 tccttaaaat caagagattt taattgtgta ataattactt gtccttttat atctagctct
    49501 tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg aagtaattgg acatatgacc
    49561 gaaaacattg ttttacggtt aaattcataa cgagagacga ctagtcctgg tcgtcttttt
    49621 tgaatttctt ttcctacgga gggatcaaaa tcaatccata caatatcacc ttttttagga
    49681 atataaattc tttcttctga cactataaaa tttctctccc ttcaggagtt aaatcttccc
    49741 attcgtcttt ctcataatac tcggcctctt ccccaccact aaatggatct tcaatttttg
    49801 gaactaacgt aattgttcca tcatcagaat acacaacgat atattcttgg ttttctgaag
    49861 gtttctttcc gttatcagaa ggtaaagtta ccactacaga acttccttgt aatctagatt
    49921 ttgtaacaag cattatcatc actcccttaa taaaagtata ctatataaca ctaagtaacg
    49981 caaaattccc ctttgtaaac tatctagaaa attgttttgt ttaaagtata tcttattgga
    50041 tataaataga aataaattgt agccagttca ttttttcttt aactaaaaaa ataatccaag
    50101 aaacattgaa cctatggatc caaaagctac ccaaatacta tagctaaccc cccgctacct
    50161 tcaattttgc agataaaacg ctttgggaga aaatttcaaa gacaacaggc catcctatca
    50221 tgcccgatta taaacaaaaa gctggctatt ccttttgctc tgacaaattt caattgggaa
    50281 agttcacgaa actagcaggt gaagaagttt caactgttcg gatcaaagaa tgcccgcttc
    50341 aaatagaaac attggttcaa cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg
    50401 agatcgtagc tatttttgta gaagaaggga ttttacatga tccaactcat atcaatgtgg
    50461 ataaatggca acctcttatc tataaattca gagaatatac aactgccaac cagtcgttaa
    50521 ctttgaattt tagattccaa gaattcaaag agtcataatg ataaacatcg ttaggtataa
    50581 ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag ctatacatta gaaaggtcga
    50641 ctgtgaccct atagatgcag tatatgcagc ttatgagcat ttcttctgtt ctctttgttt
    50701 ttgggatctg tgctgaagtc tttttcgttt tttgccgctc tttctttgaa aaatcgatta
    50761 tgttatacct tctccattac taattcaggc atctgctgag taatgatttt tcgcgcaaaa
    50821 gaaatatctg ttacccaagg ttcgatttgt tcttcagtca agacaagcgg cattcggtca
    50881 tggattggtg cgactgtgcg gttaggttca gtagtcatta tgattgattc tgccattttt
    50941 tcatcctgat ggctacgata aaaaccgcca atatacacta ttttttcctt atttgaaaac
    51001 ataaatttgt ctttttcttc attccactca taaaagccac tcattgggaa aacacaacga
    51061 ttttgcaaaa atggttgatg aaaggtcttt ttttcttcta ctgtctctgc acgggcatta
    51121 atcatcaatt gtcctttttt aaatccggta aatccccact tcatgatttc cggagtgagg
    51181 atgttttctt cctcttttct taacgtcaca acaagatttg acggaaaaac ttccccagct
    51241 gctaattctg ctgcctgctt cggttgtttc gtctgggctt ctcgataata attctgaagg
    51301 agctctgact ctagatcaaa aaaataacga ccacacatcc taatccctcc caatcttttt
    51361 ttctatcata cactgcaaca agaagttcgc accaacaaaa cgctttcaat ttttgtagaa
    51421 ctaaaaaagt tgtgacaagt gacaaactct ccagtgaatg gtattctcct gccaactcct
    51481 cggtcacaag tcacaatatt caacaaacat gagaagctgc tttttgaaat tgttccttgt
    51541 gactcggagt gaaacggcag gcttacgacc tctgggtaaa cggtgttcaa aagcttgcat
    51601 ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc tatttttatt tggctgttct
    51661 tattacttga tgcagggcag ctttttcaca acctctccac taaacggtgt tcaatcagct
    51721 aaccttcggt attagctcaa aaaacggcaa aatatgag
//
LOCUS       DACEDM010000008        48185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEDM010000008 DACEDM010000000
VERSION     DACEDM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.008"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            85..972
                     /locus_tag="IWO50_000468"
     CDS             85..972
                     /locus_tag="IWO50_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0357616.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IWO50_000469"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IWO50_000469"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0357617.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IWO50_000470"
     CDS             2274..2720
                     /locus_tag="IWO50_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0357618.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IWO50_000471"
     CDS             2993..4345
                     /locus_tag="IWO50_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0357619.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IWO50_000472"
     CDS             4516..4803
                     /locus_tag="IWO50_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357620.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="IWO50_000473"
     CDS             5029..5472
                     /locus_tag="IWO50_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0357621.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="IWO50_000474"
     CDS             complement(5478..6662)
                     /locus_tag="IWO50_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0357622.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="IWO50_000475"
     CDS             6930..7628
                     /locus_tag="IWO50_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0357623.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="IWO50_000476"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="IWO50_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0357624.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="IWO50_000477"
     CDS             complement(8438..8971)
                     /locus_tag="IWO50_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357625.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="IWO50_000478"
     CDS             complement(9268..11649)
                     /locus_tag="IWO50_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0357626.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IWO50_000479"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IWO50_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0357627.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="IWO50_000480"
     CDS             12350..12889
                     /locus_tag="IWO50_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0357628.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="IWO50_000481"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="IWO50_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0357629.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="IWO50_000482"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWO50_000482"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="IWO50_000483"
     CDS             13996..15162
                     /locus_tag="IWO50_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0357630.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="IWO50_000484"
     CDS             15208..16704
                     /locus_tag="IWO50_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0357631.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="IWO50_000485"
     CDS             16785..17468
                     /locus_tag="IWO50_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0357632.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="IWO50_000486"
     CDS             18113..19303
                     /locus_tag="IWO50_000486"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0357633.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="IWO50_000487"
     CDS             19405..20358
                     /locus_tag="IWO50_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0357634.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="IWO50_000488"
     CDS             20427..21776
                     /locus_tag="IWO50_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0357635.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="IWO50_000489"
     CDS             complement(21959..22282)
                     /locus_tag="IWO50_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357636.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="IWO50_000490"
     CDS             22411..23574
                     /locus_tag="IWO50_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357637.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="IWO50_000491"
     CDS             23644..24993
                     /locus_tag="IWO50_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0357638.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="IWO50_000492"
     CDS             complement(25038..25469)
                     /locus_tag="IWO50_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0357639.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="IWO50_000493"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="IWO50_000493"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0357640.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="IWO50_000494"
     CDS             28699..29295
                     /locus_tag="IWO50_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0357641.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="IWO50_000495"
     CDS             29417..30565
                     /locus_tag="IWO50_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0357642.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="IWO50_000496"
     CDS             complement(30603..31016)
                     /locus_tag="IWO50_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0357643.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="IWO50_000497"
     CDS             31095..31526
                     /locus_tag="IWO50_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0357644.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="IWO50_000498"
     CDS             complement(31625..31858)
                     /locus_tag="IWO50_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0357645.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32041..33711
                     /locus_tag="IWO50_000499"
     CDS             32041..33711
                     /locus_tag="IWO50_000499"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0357646.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="IWO50_000500"
     CDS             33858..34244
                     /locus_tag="IWO50_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357647.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="IWO50_000501"
     CDS             34231..34929
                     /locus_tag="IWO50_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0357648.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="IWO50_000502"
     CDS             34934..35398
                     /locus_tag="IWO50_000502"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0357649.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="IWO50_000503"
     CDS             35395..36306
                     /locus_tag="IWO50_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0357650.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="IWO50_000504"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="IWO50_000504"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0357651.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="IWO50_000505"
     CDS             37094..38377
                     /locus_tag="IWO50_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0357652.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="IWO50_000506"
     CDS             38390..39316
                     /locus_tag="IWO50_000506"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0357653.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="IWO50_000507"
     CDS             39335..40615
                     /locus_tag="IWO50_000507"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0357654.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFSLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="IWO50_000508"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="IWO50_000508"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0357655.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="IWO50_000509"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="IWO50_000509"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0357656.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="IWO50_000510"
     CDS             44940..45716
                     /locus_tag="IWO50_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0357657.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="IWO50_000511"
     CDS             45713..46645
                     /locus_tag="IWO50_000511"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0357658.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="IWO50_000512"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="IWO50_000512"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0357659.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="IWO50_000513"
     CDS             47365..47994
                     /locus_tag="IWO50_000513"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0357660.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..>48185
                     /locus_tag="IWO50_000514"
     CDS             47982..>48185
                     /locus_tag="IWO50_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357661.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRII"
BASE COUNT        15863 a         8394 c        10354 g        13574 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagcg ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tagaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaatt aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattct cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taatc
//
LOCUS       DACEDM010000009        45581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEDM010000009 DACEDM010000000
VERSION     DACEDM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.009"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            252..743
                     /gene="nrdR"
                     /locus_tag="IWO50_000515"
     CDS             252..743
                     /gene="nrdR"
                     /locus_tag="IWO50_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0357662.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            766..2139
                     /locus_tag="IWO50_000516"
     CDS             766..2139
                     /locus_tag="IWO50_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0357663.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2139..3065
                     /gene="dnaI"
                     /locus_tag="IWO50_000517"
     CDS             2139..3065
                     /gene="dnaI"
                     /locus_tag="IWO50_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0357664.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3194..3634
                     /locus_tag="IWO50_000518"
     CDS             3194..3634
                     /locus_tag="IWO50_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0357665.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3778..4479
                     /locus_tag="IWO50_000519"
     CDS             3778..4479
                     /locus_tag="IWO50_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0357666.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            4476..5597
                     /locus_tag="IWO50_000520"
     CDS             4476..5597
                     /locus_tag="IWO50_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0357667.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            5612..6844
                     /gene="pepT"
                     /locus_tag="IWO50_000521"
     CDS             5612..6844
                     /gene="pepT"
                     /locus_tag="IWO50_000521"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0357668.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6976..7884
                     /locus_tag="IWO50_000522"
     CDS             6976..7884
                     /locus_tag="IWO50_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0357669.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7919..8191
                     /locus_tag="IWO50_000523"
     CDS             7919..8191
                     /locus_tag="IWO50_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0357670.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            8202..9248
                     /locus_tag="IWO50_000524"
     CDS             8202..9248
                     /locus_tag="IWO50_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0357671.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            9499..12108
                     /gene="clpB"
                     /locus_tag="IWO50_000525"
     CDS             9499..12108
                     /gene="clpB"
                     /locus_tag="IWO50_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0357672.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(12259..12930)
                     /locus_tag="IWO50_000526"
     CDS             complement(12259..12930)
                     /locus_tag="IWO50_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357673.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(12923..13417)
                     /locus_tag="IWO50_000527"
     CDS             complement(12923..13417)
                     /locus_tag="IWO50_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0357674.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(13437..14657)
                     /locus_tag="IWO50_000528"
     CDS             complement(13437..14657)
                     /locus_tag="IWO50_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0357675.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            14804..16639
                     /gene="lepA"
                     /locus_tag="IWO50_000529"
     CDS             14804..16639
                     /gene="lepA"
                     /locus_tag="IWO50_000529"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0357676.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            16835..17746
                     /locus_tag="IWO50_000530"
     CDS             16835..17746
                     /locus_tag="IWO50_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0357677.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            18745..18996
                     /locus_tag="IWO50_000531"
     CDS             18745..18996
                     /locus_tag="IWO50_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357678.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            19079..19351
                     /locus_tag="IWO50_000532"
     CDS             19079..19351
                     /locus_tag="IWO50_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357679.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            19441..19668
                     /locus_tag="IWO50_000533"
                     /pseudo
     CDS             19441..19668
                     /locus_tag="IWO50_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19826..22126
                     /locus_tag="IWO50_000534"
     CDS             19826..22126
                     /locus_tag="IWO50_000534"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ0357680.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAKGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETSVKAQGASSLNNAVYGLYQADGKKIKEVTL
                     KNVNGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLIDSMNPDNKPKLAGIEISLTSKTTGKVVKTVVTDKDGYATFGKNAV
                     VFDTYILSETKGKEGYKLFEPFEVTISENGQTFHYALEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTFTYGKDRYRLEE
                     IKAPVNYVLNQNHWYSRLPMIQNQSK"
     gene            22084..24108
                     /locus_tag="IWO50_000535"
     CDS             22084..24108
                     /locus_tag="IWO50_000535"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ0357681.1"
                     /translation="MVFSITNDTEPIKVIHFANRQARKNVKLFKYEEWNKKKTPLAGV
                     TFTLYNQAGKALGEYITDQNGEILVKGLVANNYYFIENTPLESFQPNTDKQAFTITFK
                     QNAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGK
                     EYTISGVLMNKATNTPILQDGKEIRKTLTFIADKVNGTKDLEFVVNASVLKGQTIVVF
                     EKLFRDGIEVAAHANINDENQSVRVNNPDVHTTAVFEDGLTVADPTGLFTLNDMVKIK
                     EIIPGKAYLVKGILMDKATGEALLVHGQPVESAGGFIAKAENETVIVPFTFDLTGLNG
                     REIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKVVNPLMKITI
                     TDAVKYHDLIVGKTYTVNGILMDKSTEKPVLVDDKEITSTLTFVAERIDGTVNLDFVL
                     DARALRGKEVVVFEDLFRDGVLLASHADITDKDQTIKIVNPTITTKATNKNGGKEILA
                     LPNQTVLEWVKVDGLAIGQLYKLLVQGYLTPDGTPFEGTHAEKVFTADKEMMEFLFEF
                     LVDGRNLAGKGLSFVEWLQAKTEDDKEAFEEVVKHNVDLTNKDQTVQFVEAPKPPQPV
                     KPEQPVKAATVVPMLPKTGEQTSSILMVLGLVVLVIVLGITLYKVRKAKD"
     gene            24207..24572
                     /locus_tag="IWO50_000536"
     CDS             24207..24572
                     /locus_tag="IWO50_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357682.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            24574..25026
                     /locus_tag="IWO50_000537"
     CDS             24574..25026
                     /locus_tag="IWO50_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357683.1"
                     /translation="MGLKFPNNYDITEALDETCVGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNLKQSQEKGLDQKQPSVDSKEKEKK"
     gene            25095..25472
                     /locus_tag="IWO50_000538"
                     /pseudo
     CDS             25095..25472
                     /locus_tag="IWO50_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25641..26951
                     /locus_tag="IWO50_000539"
     CDS             25641..26951
                     /locus_tag="IWO50_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0357684.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMRWSRLKEGYFASVYWRQLLA
                     RMLIDNRFIYTKKQVGEKQMKEKMRLPKVYFYQKKQELVISFPLDGGRFHERFLKMGN
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     LLISGAIGGGKTYLLYSLIKACLDVGTVDICDGKAADLAALGDVDIFKGHVFYKTNEE
                     MIACLRNALKEMNQRYAYMKTMKEPKYEPGKNYAYYGISPHFIFFDEWAAFYGSLDME
                     TRNKVDKLIQPLVLKGRQAGMYVILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            26974..27285
                     /locus_tag="IWO50_000540"
     CDS             26974..27285
                     /locus_tag="IWO50_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357685.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVGLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            27645..28901
                     /locus_tag="IWO50_000541"
     CDS             27645..28901
                     /locus_tag="IWO50_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0357686.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKRKIPEDFEQRIKERFRLYVESGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQSYLEWMGTSGGKIA"
     gene            28898..29443
                     /locus_tag="IWO50_000542"
     CDS             28898..29443
                     /locus_tag="IWO50_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357687.1"
                     /translation="MNSYMTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLESIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLATVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            29444..29944
                     /locus_tag="IWO50_000543"
     CDS             29444..29944
                     /locus_tag="IWO50_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357688.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKRELEGQFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            30024..30581
                     /locus_tag="IWO50_000544"
     CDS             30024..30581
                     /locus_tag="IWO50_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357689.1"
                     /translation="MSLEFLGEPIEKEEFVLHYMDLFIQLIKKLCAIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFIKREKNQMSVEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEQLMMDFSNLYRGYECSMIQER"
     gene            30601..30946
                     /locus_tag="IWO50_000545"
                     /pseudo
     CDS             30601..30946
                     /locus_tag="IWO50_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30965..31879
                     /locus_tag="IWO50_000546"
     CDS             30965..31879
                     /locus_tag="IWO50_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0357690.1"
                     /translation="MIKTTLAVVTTVGITLFSFQLGTLLVNADETVETPKSEIVDTLP
                     GEELPDVETELSGGASETPEQSQPEQPEKELIESEPEFPESTLPTDPIEEEGVHGSTT
                     TDSSTVTTPEQSGTTSSSEKTTDSSSTEQPTMSSTSEESKEEPSQPAEKASEKSEAIP
                     KENKPVVPAKEVTVSVTPSGEITTNTSQGTSVPIVTSNVEELTHIPTPTTPLKVESGQ
                     TIVGVKDGIPLVQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVL
                     SVLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            31958..32950
                     /locus_tag="IWO50_000547"
     CDS             31958..32950
                     /locus_tag="IWO50_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0357691.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKGVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKIDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDTKGEERK"
     gene            32947..33165
                     /locus_tag="IWO50_000548"
     CDS             32947..33165
                     /locus_tag="IWO50_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357692.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNNPTRVFDAFGKIIEKLFMN"
     gene            33178..33588
                     /locus_tag="IWO50_000549"
     CDS             33178..33588
                     /locus_tag="IWO50_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357693.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVAI
                     FEYLFLRSFISWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            33641..36169
                     /locus_tag="IWO50_000550"
     CDS             33641..36169
                     /locus_tag="IWO50_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0357694.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGTSYLALLPIGETPENMRSCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSFVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            36185..38395
                     /locus_tag="IWO50_000551"
     CDS             36185..38395
                     /locus_tag="IWO50_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357695.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVINPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQQLGQRSSESKPSISAP
                     KSLQPAFSSLDKSTDQSKKIEKDPKTLPILDAKLGTDFTLPKGRTVQKPLSKKG"
     gene            38403..39494
                     /locus_tag="IWO50_000552"
     CDS             38403..39494
                     /locus_tag="IWO50_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0357696.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNGE
                     YTEGLPIFSEIRGRGSITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKMK"
     gene            39515..40057
                     /locus_tag="IWO50_000553"
     CDS             39515..40057
                     /locus_tag="IWO50_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357697.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            40279..40509
                     /locus_tag="IWO50_000554"
     CDS             40279..40509
                     /locus_tag="IWO50_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357698.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            40523..40855
                     /locus_tag="IWO50_000555"
     CDS             40523..40855
                     /locus_tag="IWO50_000555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357699.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            40900..41448
                     /locus_tag="IWO50_000556"
     CDS             40900..41448
                     /locus_tag="IWO50_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0357700.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            41455..41907
                     /locus_tag="IWO50_000557"
     CDS             41455..41907
                     /locus_tag="IWO50_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357701.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            42004..42339
                     /locus_tag="IWO50_000558"
                     /pseudo
     CDS             42004..42339
                     /locus_tag="IWO50_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(43064..43735)
                     /locus_tag="IWO50_000559"
     CDS             complement(43064..43735)
                     /locus_tag="IWO50_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0357702.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            44039..>45581
                     /locus_tag="IWO50_000560"
     CDS             44039..>45581
                     /locus_tag="IWO50_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0357703.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLIIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKK"
BASE COUNT        15536 a         7133 c         9617 g        13295 t
ORIGIN      
        1 tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga
       61 tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga
      121 aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg
      181 tgttataata caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga
      241 ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc
      301 gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc
      361 gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg
      421 aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa
      481 gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca
      541 gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag
      601 ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata
      661 tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag
      721 agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg
      781 aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac
      841 agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc
      901 tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat
      961 gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag
     1021 ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt
     1081 tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg
     1141 gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt
     1201 gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt
     1261 tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa
     1321 tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag
     1381 tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa
     1441 caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc
     1501 agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt
     1561 ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac
     1621 agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa
     1681 gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat
     1741 gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt
     1801 agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat
     1861 gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa
     1921 gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag
     1981 caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc
     2041 aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt
     2101 tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat
     2161 tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag
     2221 tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag
     2281 cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc
     2341 aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc
     2401 attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag
     2461 aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg
     2521 aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag
     2581 agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg
     2641 gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca
     2701 ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag
     2761 atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca
     2821 ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat
     2881 atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg
     2941 aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa
     3001 tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg
     3061 gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat
     3121 cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa
     3181 cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct
     3241 tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg
     3301 tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat
     3361 tgaaagtatt actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt
     3421 catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt
     3481 aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt
     3541 ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg
     3601 agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga
     3661 aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat
     3721 tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg
     3781 aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct
     3841 cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat attaagagga
     3901 aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag
     3961 caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat
     4021 gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg
     4081 atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg
     4141 atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat
     4201 aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc
     4261 gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg
     4321 cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa
     4381 gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag
     4441 caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca
     4501 tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac
     4561 tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc
     4621 cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc
     4681 caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag
     4741 atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg
     4801 atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa
     4861 agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga
     4921 aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa
     4981 gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg
     5041 ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa
     5101 ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt
     5161 atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat
     5221 tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag
     5281 atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt
     5341 gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata
     5401 tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa
     5461 ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat
     5521 cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc
     5581 catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt
     5641 acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca
     5701 gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga
     5761 tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca
     5821 tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta atgcagtagg
     5881 agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga
     5941 agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac
     6001 attgatcaca acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga
     6061 aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa
     6121 agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca
     6181 attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga
     6241 aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac
     6301 ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc
     6361 agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt
     6421 ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa
     6481 attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga
     6541 tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga
     6601 aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc
     6661 agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc
     6721 gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt
     6781 acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag
     6841 ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct
     6901 gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta
     6961 aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat
     7021 gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta
     7081 tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca
     7141 gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc
     7201 gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact
     7261 gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg
     7321 atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc
     7381 tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa
     7441 cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa
     7501 aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg
     7561 acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgattc agcgacccta
     7621 gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt
     7681 tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca
     7741 gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac
     7801 acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca
     7861 ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat
     7921 ggatgtattg ttacacaatc ttttgtggtt ttaccttttc attgtgaatt tttacttatt
     7981 ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa
     8041 taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt
     8101 tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt
     8161 tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga
     8221 aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt
     8281 taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt
     8341 tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag
     8401 aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct
     8461 atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt
     8521 tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga
     8581 ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc
     8641 ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga
     8701 tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg
     8761 tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca
     8821 atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc
     8881 tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt
     8941 tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct
     9001 ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg
     9061 tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac
     9121 tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg
     9181 aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc
     9241 aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat
     9301 aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt
     9361 taagatttat ataagtcgag aaaaaaagtc aaaaaaccat gtgtatattt tgaccaaaat
     9421 tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa
     9481 ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt
     9541 gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta
     9601 tggaaaatct ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg
     9661 gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct
     9721 ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa
     9781 gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta
     9841 gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct
     9901 gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa
     9961 aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc
    10021 aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt
    10081 attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa
    10141 acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta
    10201 aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt
    10261 ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt
    10321 atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt
    10381 atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt
    10441 gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc
    10501 tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga
    10561 ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca
    10621 gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat
    10681 ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta
    10741 gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa
    10801 tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt
    10861 gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt
    10921 tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac
    10981 tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa
    11041 ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt
    11101 accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt
    11161 gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt
    11221 ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg
    11281 caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa
    11341 accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg
    11401 atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca
    11461 ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat
    11521 acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta
    11581 caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat
    11641 acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct
    11701 gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc
    11761 aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac
    11821 aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa
    11881 gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa
    11941 ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta
    12001 gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt
    12061 gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag
    12121 actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa
    12181 cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt
    12241 ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta
    12301 acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt
    12361 atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc
    12421 atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca
    12481 gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca
    12541 aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag
    12601 agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact
    12661 gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa
    12721 aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat
    12781 caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt
    12841 ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc
    12901 tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta
    12961 cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta
    13021 ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt
    13081 ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc
    13141 ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta
    13201 gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc
    13261 gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg
    13321 aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac
    13381 ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa
    13441 aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt
    13501 tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc
    13561 atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag
    13621 acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg
    13681 acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga
    13741 aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc
    13801 tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag
    13861 tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca
    13921 agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct
    13981 cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa
    14041 cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca
    14101 aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca
    14161 cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac
    14221 atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc
    14281 cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca
    14341 tggttagcat attctggata agttccaact ccagtcacac cacccatttt ataacgtgca
    14401 ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg
    14461 aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt
    14521 ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc
    14581 aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta
    14641 aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat
    14701 tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt
    14761 tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat
    14821 gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa
    14881 atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca
    14941 agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa
    15001 tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga
    15061 caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg
    15121 tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta
    15181 tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc
    15241 agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga
    15301 agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt
    15361 ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga
    15421 ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt
    15481 caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt
    15541 cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta
    15601 tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc
    15661 agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg
    15721 tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact
    15781 gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg
    15841 gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg
    15901 ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac
    15961 ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca
    16021 ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc
    16081 agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga
    16141 ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga
    16201 caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca
    16261 aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag
    16321 ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa
    16381 aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat
    16441 cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg
    16501 agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa
    16561 acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggatga
    16621 gcaagatagt gtgaaataac ttataatcaa tcgaaaaacc tattaaatca acctttttga
    16681 gtagatgcaa cactgaggat tatctgtata aaagatttta aactgtcatt tggttatcac
    16741 ggcgatacta aaattaacaa aattggaata tttttcttga agatttttag ctgtcagtat
    16801 atgatgaaag catctcatag gaggtgcttt tttcatggtt gataattcaa gtggaagggt
    16861 tcttaaatct ctaagaaaag agaaaaaact atctcaaaaa aagttagctg atttagcagg
    16921 tatttcacaa agtactttag ttaaatatga aaaagggtca agaaaaatcc ctaaagacgt
    16981 agataatact ctaagcaaga ttctgaatat agaaactcta ataaaagatg aggaagataa
    17041 gattgagatt ctcatcggtc aactaattgc atacagagat atgaataagt tacttaacaa
    17101 agagttagct gataatatag gaattagtga agtattactc agttatgtgt taaatagaaa
    17161 gagaaatccg tcaaaagaaa tgcaaaaaaa aatcgatatt tttctattaa gtaatgaaaa
    17221 agaaattttg aaggagatta atcgtgatag tgagatattt agtttatcaa aagacgataa
    17281 aattgttatg ggaaaaagaa tacgagaagt ccgaaagaac cgggaagaga cgcttgaaaa
    17341 atttggtaaa aactttacca tctatacagg caaaaatgtt ataagtagat gggaaaaagg
    17401 tattaatatt ccagatattg aaaaactaat gaatattgcc tatcttggta aggtcactgt
    17461 tccttacctt atgtacggag aagattataa gaatatactt ccaaaagatg aaagggttag
    17521 cgattttaaa aagataaatt cttttagtat gggattaagg atgaggaaaa ttaggaaaga
    17581 ttattatcta gaaagggagg agtttggaaa gcttttttct ccttcaattt caaaatggtc
    17641 catagatagg tatgagaatg gtagagatat acccaatact aatcgaatta tccaatatgc
    17701 ttatatagga aatctttctt tagaattttt gatttacggg atataaaaat agtttacgtc
    17761 gatacttatt aagttatcga cgttttttta ttatagaaaa aatttataaa aggaggtttc
    17821 ttgatatgat aagaaacaaa aagtgttggt tgaaagttgc tttactggca gctttttttc
    17881 taagtaactt tttgacaagc tcaatggcaa cacaagcgga agaagtaata caagaaataa
    17941 ctgttatgaa taacagctag ataaaatggg ctaggtatgt tatccaacaa tttagtaatc
    18001 actagaagcc tagcgaaagt tcgcttgata agttatagat gagtattgat ttgtagtgtt
    18061 tccaaatcaa aagggtacag agccacatga aatatgttgt cctctgcagc gtcaggatgg
    18121 gaatccatac catggagatt cggagatctg tgagggaata cttacgtggt taatatgtcg
    18181 gttgtgttgg acgtagtaat ggtcatagtg ggtagattga ctgaattctt atatgagaaa
    18241 atgaaaccaa caagaagata gggttattta tacagaattg gggatctttt ctggaaaagt
    18301 aatcggacaa tgcgagattc gtacggtctt tcttaaattt ctcttaaaaa aaacggcatc
    18361 gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata aaaaatgatt
    18421 ctatttatct ttttacatct atcgtttttt caagtaaccg caaattgtat cagcattcaa
    18481 caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt gttgtgaaga
    18541 taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt aggttaggac
    18601 aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag taatttccga
    18661 ttattggagt ttttttattt ataaagaaga agcgaataag ttgtgattcc tagatttcgc
    18721 gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag tgtttaatac acgaggacaa
    18781 agtttcttag gtattgaagg cacacgtgta acggttgatg ggtggtgtga ttgtttcgag
    18841 gttggaaaac atgttgaact tatggcattt actgagctaa aagataagaa caacgtggaa
    18901 tttttcgaga gtgatattgg ttgggacgat catgaagaag tttatggaaa atttattttg
    18961 cggaagggaa attcttatac gaatggggaa gcataagtag cgatttgtta gagatcattg
    19021 ataggattga aattgtcggg aatatttatg agaatctaga gttatttgag gtgatagaat
    19081 gagtgatggt tttgaacaag tatcaataat taaaaatcaa gttcgggaga ttcttagaaa
    19141 aaaaggctat ttggtagata gttattttga aggagattac gaaacatggg taggtgtcta
    19201 tgctcgtcca gagaataaac ctacctattt agatcctacg acgtcagaag atgcctattt
    19261 acaaaatagg tatcgagtag atggttttaa gcaagacttt gctgagtggt ttgaatggga
    19321 aattgaaaac ggagaagtaa aagaagaata agcgtacttg ttgtatcaaa ataagccagt
    19381 agaacaacca aaccaatagc tagtaatgac aataatactg aggaatcgga tcattatggt
    19441 gtgattgata tttctcttcc agaagatctt aagccagatc ctgcaacaat tggcaacagt
    19501 ggcatgattt ttgattcgtt agatgaagct atgtcatatg gttggaaaga aattaaggat
    19561 gaaggtagta attggtatgg catgagattt ggtgatggtg aagttgccaa taaagctggt
    19621 attccaattg ggaaatggac agttgatttc cacgaagcag gttctttttt gttctgtaat
    19681 tatagtggga gggtaaaatc attgatgaaa tgtgttcaga taagagcgcc agataaaaat
    19741 agaagagaca gttcacgtcg ttatcacttt tggtaacggc gttttgtttt attatataaa
    19801 attctcagga aaaggaggaa ttcttgtgta tgcaaaaacg aaaatgaatt ggttgaaagc
    19861 actcgcattg gtggcctttt ctcaattctg tttcttggtg ggaactgctc aagcacatgc
    19921 agaagaaacg acggctacac aacctacggt ggaagccatt gcccaagtcg cttctgaagt
    19981 gacagaagtc caaccagcag aagccggggt cccagctggt gaagccactg ttgaacaggc
    20041 ccctgtgaca gaagcgccaa cggtcaattc caaaacgact ctcgatcaga cgattgggga
    20101 aacacaacac ccagggaata gtcctgttac ttcacaacca gttgaagaaa ctgcctcaat
    20161 tacaacccct gcttcagaaa cgtccactcc agaaggagaa aaagctgccg aagcaacgcc
    20221 ttctgtgaag ccagagtctt ctgagaaaca agcggaagaa aagcaacgaa tagaagaaac
    20281 agtggaacaa ccagcggcta ctgctgacaa agaaggaaaa aatgaggaaa acgttgaaaa
    20341 aacaatctct tcaaccgcag ctagtcaaaa tcaaccaacg aaaccaaaca aagaatctac
    20401 ggctagtatt gaagatcaaa agaaaaaaga gcaggacatt ccaaaaacag gtacacctac
    20461 actgttagta acagtaccaa ccaatcgttc ggttcgtgtt cgccgtgcaa ccaatacagc
    20521 tgttttaggt ggtcggaaaa ttgattgggg cgaaaatatt ggtgttactt ctagtccgaa
    20581 caatccaaac ttcactttaa attatatggt tcgttttgag attaatggac aagtggcgtt
    20641 ttgtattgag cctggtgtct cagccgcaaa aggtcaagtt tatacagcta ctgctttgga
    20701 aaactatttg aaaaattcaa atgtacggaa aaaaacaagc ttgatctctt atttaggata
    20761 tgtcacaaat aaagacaaat ccgatgaaca atacattgcg gcacaattta tgatttggga
    20821 agctttgggc acaaaaatcc caatcaaaaa tatccgtgtt gattatgatc gtcgaaaagc
    20881 agaaattcaa cgtctgctta ataattttga tgtaagagcg acgtttcata atcaaacaca
    20941 tacgataaaa attggcgaaa cgttaaaagt aaaaagtgga aatgactttt tacaacgtgt
    21001 aaaaggcatt caaaagccaa aaggtatcaa tgtatctatt catgggaatg aagcatggat
    21061 cacagtcaca aaagaagcac cagaaaaggc aaaaattcat ttcaatcaga ttgaagtatt
    21121 gggtacgcca ttagcgtatc gtcgtccagg tgcccaaaca attggtatat tgaatccttt
    21181 tgaacctggt gactctgttg tcaatttgac tattttgaaa caaggaaaca ttcgtgtctt
    21241 taaagaagat gcagaaacta gtgtgaaagc acaaggtgcc tcttctttga acaacgccgt
    21301 ttatggtttg tatcaagccg atgggaagaa aatcaaagaa gttactttga agaacgtcaa
    21361 tgggaaagtt caagcagaaa ttaaagacct tgatcctggc aaatatgttt taaaagaaat
    21421 caaagcgcca aaaggttatg tgctttctga tgaagcaatt gaaattgttg ttgaacctgg
    21481 aaaaactgtc aatgcgaccg caaaagacca agtgattaag ggcaccattg atttagtgaa
    21541 agttgctaat aaagacttaa tcgattcaat gaatccagat aataaaccaa aattagctgg
    21601 tattgaaatc tctttgacgt caaaaacaac tggaaaagtg gtaaaaacag ttgtgacaga
    21661 taaagatggc tatgctactt ttggtaaaaa tgccgttgtt tttgatacgt atattctctc
    21721 tgaaaccaaa ggaaaagaag ggtataaact atttgaacca tttgaagtca caatttctga
    21781 aaatgggcaa actttccatt atgctttaga agataaagta atcgaacaac gtcttaaagt
    21841 ggtgaaagtg gatcaagaaa caggaaaagt gattccactt gccaatacag aattcaagat
    21901 ttttgatacc ttagccaatc gttatgtcac aatggagatt ccaaatgata cagaaacaac
    21961 ggatatcttt aaaaccaatg aaaaaggctt ttttctaact aacggtacat ttacttatgg
    22021 aaaagatcgc tatcgtttgg aagaaattaa agcgcccgta aattacgttt tgaatcaaaa
    22081 ccattggtat tctcgattac caatgataca gaaccaatca aagtgatcca ttttgcgaac
    22141 cgtcaagcac ggaaaaacgt gaaattgttc aagtatgaag aatggaacaa gaagaaaaca
    22201 ccgcttgctg gtgtcacctt tactctgtat aaccaagcag ggaaagctct tggtgaatat
    22261 atcaccgatc aaaatggtga gattttagtg aaaggtctag ttgccaataa ctattacttt
    22321 atcgaaaata caccattaga aagtttccaa ccaaatacag acaaacaagc attcactatt
    22381 acattcaaac aaaatgcgcc agatgatggc gaaacgttat tggtttctgt acgtaactac
    22441 ttgattccgc caaaactaga aacacaagcg tcaaataaag ccgatggtga aaaagtggtt
    22501 gatccattag aaacgatcgt cattaaagac agtgtttctt ataccaacct gtttgtcgga
    22561 aaagaataca ccatttcagg agtattgatg aataaggcaa cgaatacacc gatcttacaa
    22621 gatggtaaag aaatcagaaa aactttgacc tttattgcag acaaagtcaa tggcacaaaa
    22681 gaccttgaat ttgtggtcaa tgccagcgtc ttaaaagggc aaacgatcgt tgtctttgaa
    22741 aaacttttcc gtgatggcat tgaagttgcg gcgcatgcga atatcaatga tgaaaatcaa
    22801 agtgttcgag taaacaatcc agatgttcat acaaccgctg tatttgaaga tggattgacg
    22861 gtcgcagacc caacggggct gtttacctta aatgatatgg ttaagatcaa agaaatcatt
    22921 ccaggaaaag cttacttggt caaagggatc ttaatggata aagcaactgg tgaagcctta
    22981 cttgttcatg gtcaaccagt agaaagtgct ggtggcttta ttgccaaagc agaaaatgaa
    23041 acggtgatcg taccatttac ctttgatttg acaggtttaa atggtcgtga aatcgtcgta
    23101 tttgaaagtc tttttagaaa tgatgaagaa ctggcaaacc acaaagacat caacgacaaa
    23161 gggcaaacgg tacgagtaac gaacccaagt gtacgtacaa cggctacgca taaaccaact
    23221 ggcttgaaag tggttaatcc attaatgaaa atcaccatta cagacgctgt gaaataccac
    23281 gacttaatcg ttgggaaaac atatacagta aacggtattc taatggacaa atcaacggaa
    23341 aaaccagtgt tggtagacga caaagaaatt actagtacgt taacgtttgt ggcagaacgt
    23401 atcgacggta cagtcaatct agactttgtg ttggacgcac gtgcgttacg aggaaaagaa
    23461 gtcgtggtct ttgaagatct cttcagagac ggtgtattat tagcttctca tgcggatatt
    23521 accgacaaag atcaaaccat caaaatcgtc aatccaacca ttacaaccaa agctaccaat
    23581 aaaaatggtg gaaaagagat cttggcttta ccaaatcaaa ccgtgttgga atgggttaaa
    23641 gtagacggtc ttgcgattgg tcagttgtac aaactgcttg ttcaaggcta cttaacacca
    23701 gacggcacgc catttgaagg tacacatgca gaaaaagtct ttacggctga taaagaaatg
    23761 atggaattct tatttgaatt cttagtggac ggtcgcaacc ttgcaggaaa aggcttaagt
    23821 tttgtggaat ggcttcaagc caaaacagaa gatgacaaag aagcgtttga ggaagtcgta
    23881 aaacataacg tggatttaac aaacaaagat caaacggttc aatttgtgga ggcaccaaaa
    23941 ccaccgcaac cagtgaaacc agaacaaccg gttaaagcag ctactgttgt accaatgttg
    24001 ccaaaaacag gcgaacaaac aagtagtata cttatggttc ttggattagt agtattagtc
    24061 attgttttag gtataacact ttataaagta cgtaaagcca aagattaggc atgaggaaca
    24121 agcggttagg atttttaacc gcttgtttga ttgtaatgta gaaatattta gaatttattt
    24181 tggtttttaa gaggaggaaa aactttatgg cagaaaaagc attgcgtatg aagaaagagt
    24241 ttttaggtca agtattttta ttgggttcac gggaacctaa tccaccaaag gaaattaatg
    24301 gtgtgtctgg ccgatatgtt caattacaag cagaatgttc gcgagaagaa cagcctgctt
    24361 tttttgttta tctcacagct gaagaggata aaagattacg attaccagaa aaagaggtgc
    24421 gtatttcatt tgaagaagct tattttgttt ttcgtaaaga acgagtgaat agtcgtgaaa
    24481 caaatgatat gggacaaaca gagcgtcgaa caactggcta tcaagatgtt ggtgtctttt
    24541 taactggcgt aaaaattgag gaggttttgt aagatgggat taaaatttcc aaataattat
    24601 gatattacag aagcattaga tgaaacttgt gtaggagccg tacgctttgg cgcattagaa
    24661 gagtctgatc gtgtatttga taatgaaaca ggcgattata cgggagaaat tcgatcacag
    24721 cgagcacaat tagtttttgg aaatccagat gaaccaaaga ttgcacgacg agttaaattt
    24781 ccagcagatg tagatttgac tggttttaaa cgacgtgatc ctgtgaaatt aattgatcca
    24841 gaatatcatg tggtatatat tccagcacaa gatggcaatc gtgaatattc agatttagtg
    24901 atttatgcga aaggattaga gaaagtagaa gctccaacaa ataatcctaa tctgaaacaa
    24961 tcacaagaaa aaggtttaga tcaaaaacaa ccaagtgttg attctaagga aaaagagaaa
    25021 aaataaggat tttttgaaag agtaggaaga agtcttccta ttctttttat ctaaggtaga
    25081 aggaagggga gattttgaat tcatataaaa ccaatcaaac acttcgagag attgtggctt
    25141 gttgtcaaaa tacccaacaa ttgattgagt attttggaga caatccgacg atttttgcac
    25201 aaacaaatgc atatatttgt gcggttattg ggcagtgtat gtttttacgt gatcttgttc
    25261 gtttttcatt accatatcag tggaaacaaa atcatcagga agaggtttgg attcgtgtag
    25321 tacgaatatg taatatgtgt gcacacccat aaaaaaatcg attagatcgt tttcaattat
    25381 ggttttttat taaatgtgat attccagtat tagttgcttt ttgtcgaaag tttttagcac
    25441 aggagaagta taaacagaaa ggaggaatgt gacaattgga acctcatttt tttaagtatc
    25501 gtggtcggcg aattcgttat ttcaatcgtt atttgtggtt ctgttattat ggtttctttt
    25561 tactcccatt tttgattctg ttaggttggt ttagttggaa agtattttat ccgcgtttaa
    25621 gtacgtattc gaattggcaa atgtatctta ttcctggaat aagtattgtg ttgtatttat
    25681 tgctggttag tggattaatt ttaggtttga tgcgttggtc gcgattaaaa gaaggatatt
    25741 ttgcctctgt ttattggcgg caattacttg ctcgaatgtt aattgataat cggtttattt
    25801 atacaaagaa gcaagttggt gagaaacaaa tgaaggaaaa aatgagatta ccaaaagttt
    25861 atttttatca aaagaaacaa gaattagtga tttcttttcc tttagatggt gggcgtttcc
    25921 acgaacgatt tttaaaaatg ggtaatttgt tatctgaagt atttttgcgt gatttaattc
    25981 gtgaagaaag agaaaaagcc cgaatcaatt atgtatttct ttccgattct ggtcggattc
    26041 ctattgatca atgtatagca gaaaatggac gtattcattt aatgaaaacc ttggattggg
    26101 tatatgatca atcgccaaat ctgttaattt ctggtgcgat tggtggcgga aaaacctatt
    26161 tattgtattc attgatcaaa gcttgtttag atgttgggac agtagatatt tgcgatggaa
    26221 aagcggcaga tttagcggct ttaggtgacg ttgatatatt caaaggacat gtgttttaca
    26281 aaacgaatga agaaatgatt gcttgcttac gaaatgcgtt aaaagaaatg aatcaacgat
    26341 atgcctatat gaaaacgatg aaagaaccta agtatgaacc agggaaaaat tatgcttatt
    26401 atgggatatc gccgcatttt atcttttttg atgaatgggc agctttttat ggttcgttgg
    26461 atatggaaac aagaaataaa gtcgataaat tgattcagcc attagtatta aaaggtcgtc
    26521 aagcaggcat gtacgttatt ttagcgatgc aacgaccaga tgcggaatat tttccaagtg
    26581 gtgttcgaga taatttaacg ttccgtattt ctgttggtcg attatctccg attggctatt
    26641 taatgacttt tggtgaagat tttaaaaata agccatttta caacaaagat caacctggtc
    26701 gtgggtatgc ttttccagga tttgatgtac ctgtagagtt ctttgcgcca tttgtttaca
    26761 aagactatca ctttatcaaa gaatttgaga aatttactga aatgattgtc tatgatttta
    26821 cagtaaaaga ggatagtcaa gatcaaacga tgggagatga taatcagttg ttacaagaac
    26881 aactagagcg ccaaatgtat ttagcaaaag atccaaaagg aacaagtttt tctgatgcgc
    26941 atattttgta atcaactaag gaggtgaagt taaatggcag agagttgtta ttatcgagta
    27001 gctacagcaa tacgaatgat taatccttct ctttcttcca gaacgtttta tgattggttg
    27061 aatcgtattg aacaagtaac tgattaccgc tttttgcgca aagaacgtgt ttttacagga
    27121 aaagttatta atcaagtatt gctaacgaaa aaagatatag aacgattgac tcgattgtat
    27181 cattatcgag taggtttaga agaggattta actttgtcaa tttatcgcgt tttttctcct
    27241 gaaaaatata gtgaaataac aaaattagat catttaattt tgtagttatt atctatcggg
    27301 agggctctag acgtagtctg gtagccgcac tctgcctggc ggtgcgcatt aaggctgcga
    27361 agcatgggcc tataatgcgc actcgctagc agcgtgtgca gctcacaaag aggtcgtgcg
    27421 cttgcgcacg gcctctttgt gtaaagcccc ccgatatata atatgggggg cagaaataac
    27481 ataacacaaa taaaaaagta tatttaaagc agttatatca aggctttagc taaagttaga
    27541 agaagtaccc ctttttagaa aaatcagggg gtaccaccta tgataccggc atttctagca
    27601 gattgaaaaa atcgaaattt gttattttat aaaggaggta gaatatgacg ccaattgaat
    27661 tgcgaaatta tcgaaaaaaa ttgagtgcta ttcacgcttc aggtggaggg aagaagttac
    27721 tctctcaaaa acgttttgca cagttattgg gatgctcagt agattatata aagaagatgg
    27781 aacagggaaa aaggaaaatc ccagaggatt ttgaacaacg aatcaaggaa cgctttcgat
    27841 tgtatgtgga aagcggaata gatcttcgag caatgattga ttatttgcgt ctttcctttt
    27901 ttgggacaga ctacaaaaca gtgattcaac gtgtattacg aatggatctg acagaatttg
    27961 aaacacgtcc aacgggcttg tatctttttg actgtctttc tgttcgtggg aatatctggg
    28021 tgttctggca ttcaaaacga accgtaacag aaaatgtact tgtacaattt tcaggacaag
    28081 gttgccggga atatgaggaa gtcatgcgtg aacaacaaac agattggcga acatggctat
    28141 attctatttg gaaaaatcaa ggaaagttgg aagctggtta tacacgtgtg caatgtagtc
    28201 gattcgatat tgcgttagat gagctttggg gattggaaac ggaagaacat ttcgatctct
    28261 ttgaattact agaaaaacaa aaagcgggtc tattagaaat ggattttaag agattccaga
    28321 atattggcgg ttgtttttta gatgatggct attttcgaag tgaaggatta tccttgtatt
    28381 ttggatcgcg aaagagtcca ttgtttttca atttctatga aaaacgtttt gaaattgcga
    28441 accgagaaaa aatttcagaa ttagaagcat taacgaaata tgggatttat aatcgatatg
    28501 aattacggct agccaatgag aaagcaacac aagcagtaga agcatttatt ttaggtgatt
    28561 caccgaaacg tttaggagag attggcgttg gtttaattaa tgcaaagctt attgtttggg
    28621 atcgactgga aagtgatgca cggattgttg atgaacgatg ggcaaaattg tttcgttcga
    28681 tggaagaatt ggattttaca gtaaagccag aaaaattctc attagatcgt tcagaacgct
    28741 ggtttttgaa acaagtagct ccgacattaa aattgttaca agaagcagat cgtattttag
    28801 gaagagactt tgtacaagaa ggcattgata atgctgaact aaatgacgag caacaaagct
    28861 atctagaatg gatgggaacg tcaggaggga aaattgcatg aactcatata tgacaacaga
    28921 atggttgcgc ctgttggatt taaaagctgc gatcgaagca ttaaatgaaa agatggttga
    28981 tttgtcttat tttcgttttc gagttcctta tattgaacaa gcggtaaaag ctggacgtta
    29041 tcaggaaaaa gagaactggc aagaaattgc acgcttatta gaagttcgta agggatacga
    29101 acaagaactc gaagagttgg aatttagccg aaggaaaggt aggctagaat ccattcgctt
    29161 ttatcgattt tcattgccaa tacctgcgat tttagcggtt aaaaaaggct gcgataaaat
    29221 gaagatatac gaaaactgtg tggcagcact tagcagcgaa aaacctctta tggaagaaat
    29281 ttcattggcg actgtcactt gggaaatgag tcgccagcca acagaagaac agacatatct
    29341 ttctttagaa gagattgaga tagaattaga ggaaattggt cgctatgcca cttgttctac
    29401 ctattgtgga tcggtaatta gtattgctgg tgtgattgtc taaatgaaaa aaattatgat
    29461 ttatgttggt gcgtattggt cacgtgatcc aactgtatta gaaaatccag aggcagtaca
    29521 ctattgtttg cgccaattat tctatctgta taaagaacga ttagaatcgt tgattcggca
    29581 gttgccatat actgacagac gtttagatga actgcttcta aggtatccag caatgtataa
    29641 acgacgaaaa aatagattgt taccagaaga gtatcccatt gaaaaacgtg aacttgaagg
    29701 gcaatttgtg gcttatttct atgatgatgt tcgtatgcgc ttagtagaac aacgaatgga
    29761 aatagaaaat gatcgctact attttatcaa ttattgcaag aagcgtaagt atcaagtaac
    29821 tgatgatttt tatgattgta ttttacaaga tggggaagtg attctatcca aaattgctcc
    29881 aagttttcgt gaacttgtgc cgattgattt tttgaaaaaa tgtcatttgc ggattcttcc
    29941 gtaaaaaaga gctataaaag gaaagaaaag tagaagccaa atcggtttct gcttttttat
    30001 tttttaagga agcagaagat ggtatgtcac tggaattttt aggagagcca attgaaaagg
    30061 aagagttcgt tttgcattat atggatttgt ttattcagtt aataaaaaag ctttgtgcga
    30121 ttgatgaatt ttatccgcgc gaacaagact atctagccgc tgaacgaata aaaatcagtt
    30181 tattatggga aaaattcaca gatgaaaata accgaccgcc agattatcgc tatttatcag
    30241 attgtttaac gaataatgtt ctttccaaag agtatctatt tatcaaacgt gagaaaaatc
    30301 aaatgtcggt tgaacatttt tgtgattgtt atattgcatt attgcgtagt caacagttat
    30361 taacggtaga acgatatggt gcagagtttg gttatgtcat gcaagctgag cgtcatagag
    30421 ctacaagatg ctatgaaaaa gaagatcaag tgattgatgg ctatgaagaa ttatgtattc
    30481 gaaacaatcg ctatttgcaa caacgtttat atgaacaatt gatgatggat ttttcaaatc
    30541 tctatagagg gtatgaatgt tcgatgattc aagaaaggta gaagaaaagg gggaattttt
    30601 atgtggcgat tattattttt ggtggtatgc ggtatggcat tgttctatgt gtatcgtgtc
    30661 tatctctatc cacgaatgga acgatggaca agttggtata gagatccttt aaaacaacga
    30721 aaaggattga agcggtatcg ctatgaatta cttgttgaat tttcagatgg gcgacaagca
    30781 cagtattatt ttttttgcaa actatccaaa atacaacacc agtgaactat tgattcaatt
    30841 attcaaaaat aatccatatg ttgggttaga agaaaacgga cgagagttct tttatcgttc
    30901 agataatatt agtgcggcgt ctatgaacaa aacacgaatt gcataaaaaa ttggaggaat
    30961 aatcatgata aaaacaacat tagcagtcgt aacaacagta gggattacac tattttcgtt
    31021 tcagttagga acgttattgg taaatgcaga tgaaactgta gaaacgccta agtcagaaat
    31081 tgtagacaca ttgccagggg aagagttacc agatgttgag acagaactaa gtggaggagc
    31141 aagtgaaact ccagaacaat ctcaaccaga acaacctgaa aaagaactaa tagagtcgga
    31201 accagagttt cctgagtcaa ctttaccgac tgatccaatc gaagaagaag gtgtgcatgg
    31261 ctcaacaaca acggattcaa gtacagtcac aactccagaa caatcaggaa caactagttc
    31321 ttctgaaaag actaccgatt cgtcaagtac agaacagcca acgatgtcgt ctaccagtga
    31381 agaatcaaaa gaagaaccta gtcaacctgc tgaaaaggca agcgaaaaat cagaagcaat
    31441 tccaaaagaa aataaacctg ttgttccagc aaaagaagtc acagtatcgg taaccccaag
    31501 cggtgaaatt acgacgaata cttctcaagg aaccagtgta ccgattgtca ctagtaatgt
    31561 agaggaattg acccatattc caacaccaac caccccatta aaagtagaaa gtggtcaaac
    31621 aattgttggt gtaaaagatg gaattccatt agtccaagat agtgaaggga atttggtaaa
    31681 ggatttgtcg attcctgtaa agaaacttcc aagtggcaat attgaagtca aaacagcaga
    31741 tggaaaaaca aaagtcttac caaagactgg tgaagaaata catgttgtac tttctgttct
    31801 aggaggagtg ttgacaagta ttgctggatt tgttggatat aaaagaaaga gaaaaggaat
    31861 agaaaatgag ggtaattgaa tggactattt ttcagaaaaa caagcttatc gttgtcaatc
    31921 gtacgtacta gtaaaaacat actagaagga gtagcttatg aaaaaacgac aactacggcg
    31981 gaaagaaaaa gtttatacag agaaagtacc gaagcaaaag aaaatccatt caaaaaaagc
    32041 agtgaaatgg atttggtgtt tggttttatt tctttgtttt tcagggggct tggcattttt
    32101 tcaatccatg aaaacgaagg ttcaagtgtc gtttttgaaa gatgaaatcc aacaattaca
    32161 aaagaaagaa gtacaaagtc ggtcagaatt aacgtacaca cctgaaattg aatcctttat
    32221 gaatcgtttt gttgctttgt atatgaatac attcgataat catgaacaag aacaacaacg
    32281 tcaagaaaca ttaaaaaaag agttttatgc gcaaaacttt attcaagaac caggaagtgt
    32341 ttcaggtgtt cgaaaattaa ctgctgcttc gtttattgga ttaaaagaaa ttgaaaccat
    32401 tccaacagct agttataaag taacttatga tattcgctcg atgaatccag agagtaagaa
    32461 agaaacaagt gtgacaatca cacaagtact caatcttccc ttttctattt caaacaaggg
    32521 ctgtcgtatt attgcttatc cttattttac ggcactacca aagaatacag aaaaacaacg
    32581 aatgattact tatgatgaca cattatacga aacggttgat gcgtcggaag cgaaaggtgt
    32641 tgaaggattt gtggaagatt tcttagataa gtatgcgaaa aacaatgcaa aagatatgtc
    32701 atacatgatg aaagaacctg aaggactcaa tcagacagct aaaattgatc aagtagagat
    32761 gaaagtattg cgttataaag aaacgaaaaa accttttatt gtaaaagcaa cggtcgtctt
    32821 tcgagatggt gatacgtcac ttttacaaaa agaacaaatg acgcttgtta ttacgaaaaa
    32881 aacaaatcag tattatgttg aaaagctaac acataattgg ttggatacga aaggagaaga
    32941 acggaaatga cactgaaggg aattgtagat tttatattaa cgcaaggtgg ctacttttta
    33001 ataatggtgg ttgtttatct ggggttacga aattttatga aatcaaaagt aggaaatgca
    33061 ttaatatcta ttgcagcagg tgcagcagtc tatttctttt taaataatcc aacacgtgta
    33121 tttgatgcgt ttggtaaaat cattgagaaa ttatttatga attaggagaa tagaaacatg
    33181 gattgggaac cttatgatta ttcaattgtt tatgcggaac cttatcgcgt acaacagatt
    33241 acaagcaatt ggcgtcttcc atttccagtt gcattagtca atgcagcaac ctttttgttg
    33301 gtcgcaatct ttgaatattt atttttacgt tcgtttatta gttgggttat ttcttttttt
    33361 ccaccagcag gagttgtttt ttatataggg ctaccctatt tatgtgtgtt agcactggca
    33421 aaactaaaac ctgatggaaa aaaagtgcat ttttatttat gggattggtt ttgttatgta
    33481 gtgcgtatga tgaatggtga ttgtgtgtat tataactggg acaaggtatc aaacaatgca
    33541 ttggaagaat tactttttgt gaataagtat gtttatttag aggattagaa aaagggacaa
    33601 tcaactggtt gttccttttt ttattgggag gtgaaactta ttggtgcttg taaaagaacc
    33661 agtaaaagca tttaagaaaa attgtttgtt aaatggcgag gattgtatat gggcctacta
    33721 tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg aaataaaaga
    33781 aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt tggtgatgta
    33841 tccacgaaca atgaatttac gaacaaagtt tgacacctta ggtaagggat tttcacctga
    33901 tagtcaagcc attggtgcgt attatgcaaa agaaacagtg acattgttag aacgagaagg
    33961 gcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag caacggtaga
    34021 gagctttcat gatgtattaa atctaatctg gttagatgta gcaggaagtt tagcacgctt
    34081 atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc cttatgtaaa
    34141 agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa aagagctgtt
    34201 gtatctcaat cgtctgcaat atattcgagg aatggctcat aaagtagaag aacaaaataa
    34261 ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg aacataaagg
    34321 ccttcttcaa ttagaagatg aattggggac tagttattta gcgttgttac ctattggaga
    34381 aacacctgaa aatatgagaa gttgtgatct atttcaattt gcacaggatc tttctttccc
    34441 agcagaatta cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag gtattggttt
    34501 aaaactgaat tggctatcgg aacggttcaa aacagaacat aaggaattgg ttcaaaatga
    34561 tgaagaacct agtgaacgct tattaactgt tcggggatta gtaaaacatt taaagcaacg
    34621 agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat acggtcaaac
    34681 ggttgaagaa gttcgtcaaa atagtgcaac gattattcaa cagcttcata cgattatcca
    34741 tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat taggacaacc
    34801 attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg gtgaatggtt
    34861 gttttcagca gtatcagagt taggtatgga atctggctgg tatattggtc gtcaagatac
    34921 attaggggac ggtggttcta gaggagagaa aacaccgtta gaagaattga tctacgcgtc
    34981 aaatcgcttt gtctttttaa atcttttagc cattgcggaa ggaatcaaag gtgcgttgtt
    35041 tgatgcgcct catgtagcag taactggaaa aactggtaaa gggaaaacat ttttagttgg
    35101 cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtag atcccaaagg
    35161 agaaaagcgc aaatggttca cgaaactcat tcaagacccg tattatcaga aacattaccc
    35221 tttgtttgtg gaacatttgc aaaaaatgag ttttgtcacg ttagatgcta caaatccccg
    35281 taattatggc gtattagatc caattgtttg tttatcccga gtagatgcga aacaagtagc
    35341 ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa aaggggcggt
    35401 attaaagggg atccaagaag ttttagttca aaaagaagcc gggcaacaag ttggcttgat
    35461 gcatgtggtc gagtatttag aacaaatgga acaaaaagag gttcgagaat atggtgagtt
    35521 tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg gagaaaaccc
    35581 aggattagac tttaatgcca aaacaacgat tttagagatc caagatttga aattaccaaa
    35641 tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt gtttgatgat
    35701 tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga aaacagcgat
    35761 ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta ttattgcagc
    35821 aatggcgcgt gtggggcgtt cacagatgaa tcaattagtg ttagatacac aatttattgg
    35881 cgacttaggt tcagataaag aaaaagggaa ttttggtgtt gtcttcgctt ttgatgaaga
    35941 cagtgagcga gaaaagattt tgactcattt acgtttggaa gtaaatgagg cgaacttatc
    36001 tgaaatgagc catatgatta aaggacagtg ttggatgtta gatccctatg gacgtgtagg
    36061 aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa caacggaaaa
    36121 aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga aatgaggtga
    36181 aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt ttttaacgat
    36241 tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc aggtggatca
    36301 atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt tatctgatag
    36361 tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc taaaaatgat
    36421 gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt ttgtcattga
    36481 gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca ctttttcgga
    36541 tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct tgtatgtgtt
    36601 atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg tgtttattcg
    36661 atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta acgtacctag
    36721 taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag aaggctttat
    36781 tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg gtgttttagt
    36841 gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata aagcggctgt
    36901 tgttgatccg tatctattgt taaattatgg gacaacaaat ttagaagaat tgtatcaagc
    36961 agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat tatctgatat
    37021 tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg cttatcgtgt
    37081 ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg tgccattgct
    37141 taatctaagt attggattac cacttgtagt gattggcatg attcgtttta tctttcaaat
    37201 gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt ttgttccagt
    37261 atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt ttcaaaaatc
    37321 tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag acgatttaat
    37381 tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac tcctatcaat
    37441 tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag ctgcagatca
    37501 aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga aacaactgcc
    37561 tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat tagcagggaa
    37621 tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc aaaagtttca
    37681 acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtgattaatc cctttgaact
    37741 aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt atcatggggt
    37801 agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc tttcaaatgg
    37861 tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac aatcgttggc
    37921 acaatcgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata aaggtatcgc
    37981 ttttgttggt caatcgattc cgttgcctgc tggaaaagta cccgtgccac aattaacagc
    38041 tgctggttct ctttcccaac agattcgcca agtgaaaaat caggcgaaaa atgggagtta
    38101 tccagcaatg cagcagttat tgaatcaata ctctgttcct aagtcgtcgg tagccactct
    38161 atcaggaaat cgttctgtgc aacaactggg acaacgttcg tctgaaagta aaccgtcgat
    38221 ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagtcaa cagatcaatc
    38281 gaaaaaaata gaaaaggatc ctaaaacact gccgattttg gatgcaaaac taggaaccga
    38341 ttttacttta ccaaaaggac gaacagtcca aaaaccattg tcgaagaaag gatgaggaag
    38401 aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt tttctattgc
    38461 cattcttttt ttcaggagaa ctttccgagg atggaggaga ggctacaggt ctagctgtcg
    38521 cttttaatgg ggaatatacc gaaggattgc cgattttttc agagattaga ggacgtggtt
    38581 cgatcacaga tgaacatgcg caagcagcag ttggtgcggc ggtaaagtat cagttgttgc
    38641 cgagtgtgat tctttctcaa ttgggttatg aatcggctta tggacagtca caatctggaa
    38701 gaaacgatac gaactttttt gggattacct ggtttccagg ctgtccctat ccacaaggtt
    38761 ctgcacgggg gattggcggt tctgaaggcg gcttctatat gaagtttccg aatctaaaag
    38821 cttgttatga ctattacggc tttatggtgg cgagtcaaag caactttaat caatgtgtgg
    38881 gcaataaaga tcctgggagt tgtttattaa ttctaggtcg tggaggctat gcggcggcag
    38941 gtattaccga aggcagtccg tattaccaag gagcgatgag tatcataaag tcttataact
    39001 taacagaata tgatactttt gcgatcaaac attggaatag cctaccaaca aaaaaatcgt
    39061 cacttgttgc agggaaaagt aaaggtaatg cgaaagtttt agatcggatc attggacgat
    39121 cggtaaataa tggagagtgt tatggtggca cggcgtggta tgtatcccaa ctaggtggcc
    39181 cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat tatgattgga
    39241 cagcctatgg ttggcaagtg atcatgaacc caaaaccaag tgatttacaa gcaggagatg
    39301 tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac accggaatta
    39361 taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat gccgagcaag
    39421 gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt agtctggttc
    39481 gtaagatgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa ttttagtatt
    39541 tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg aaaaacaaaa
    39601 aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa gtggaactct
    39661 tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag cgcaacaaat
    39721 gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat ggagtaattt
    39781 cacagatgtt tataaacgca atcaagctgt gagggattat atgacagatc gttgtattaa
    39841 agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga aactttacca
    39901 ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag aaacaatgaa
    39961 taaggttact caatttgtat tacttcaatt gaaagtagat caagaagctc aaaagattga
    40021 tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaaaatgaa cactcatatt
    40081 tattatacac cgaagaaata gaaaaaaaca gactgttttt ttaagaaaaa ctatgtcatt
    40141 ctttacttga aaaaagttgt tataggacag aaaggataaa acaaaaacgg aaaaaatatg
    40201 tcttatgaat aactataaga actattctat tttttaggag atggatacga gatgaaaaaa
    40261 ctaattggct atagtgtgat gtgtattgga tttgttggtg tgggattgtt tgtatcgact
    40321 gaatctgctt ctgcggctga atggtataat cctatctggt ataatccgaa cattgaatgt
    40381 caaaaaggct atccgtatgg accgacaaat ctgaaaaatg gaaaatgtag agtaaattgg
    40441 aatggcgttg tagatagtgt aattactggt tcaatggact ctgctctaca tggctttacg
    40501 ggtcactaga aggaggaaga aaatgtctga agagaaaaaa gaaatcgttg aatcattagt
    40561 agctttaaaa gattctcttt ctaagattga atatgttgat aggcaagaaa ttcaaaaact
    40621 aattgatgat actattattg aaatccaaga tgcgcgttgt gaaggtataa aaatttctgt
    40681 agctttatct aaagtaatag aaaaaatgaa ccgttcatta gcgtttaatg gattgaagct
    40741 agatagacaa actagtctga tatgggatca tttaaaagac ttgtatgaca agtctaaaag
    40801 aagtgaacgg actgctgtat caatcttaaa aggcctttgg ggaatgaatt cataaacaag
    40861 atatttaaaa agtaacttat tttttatgga ggagaaatta tgaaaagaat aattaaaata
    40921 tttggtgttt taggtgtttt cttattaggt ttaagtactt ttgttagtac tggaacaagt
    40981 gtgtatgcca cagaaaaagt gaatacagtt gatttagaaa taaagaataa attagtagat
    41041 gaattaactt tttattttga tacagttggt tttttagacg aacatggtgt ataccatata
    41101 aaggattttg gtgctattcg tgaacgaatc agtcaagata acgaacaagg aagagcagca
    41161 agagtctttt atgaacatat tattgttcct agaatggaga aagttgattg gaaacgatat
    41221 gctggatgtg tagccattaa ttctcttcct ggaggaggag tggcttgggc tataggtacg
    41281 cagggagatt ttattgaagc attaaaagct tttaattttg aaaaagctgc ggatttatta
    41341 ttaaaaattg cagaaaaaca attaggaaaa gctgcagcta aagagttagc taaggtaaat
    41401 attgtagcag gaattgcagc agcagctgct agctgtgcat tagagtaaaa gtttatgaaa
    41461 agtttagaac aagagcatat ggcttttaaa caagcaatgt ttaaagaaaa tatctattta
    41521 aaccataact atattcgtgt ttctaaggca tgttcaccag tattgaatat gcttggaggt
    41581 ggaaatggat tatatcattt actgtttgtc gatgtttgtt ggttaatttt tttaccagat
    41641 gaactagtta tagtcaatga aaaaataaca agtaaaaatg aagtgtttaa ttattcttta
    41701 acaagaatta attacaaaga gattaccaag ttttcattag aaaaagttcc tttttgggga
    41761 gaattttgtt taaaaattaa atgtaactgg aaaagaatgt atttctatat tgatggtgat
    41821 gatgctctta cattcggtaa aactactttt tctagtttta attttcagtt tctattaaaa
    41881 aataattttt atggtttatt gaaataaggt tgcgaaggaa gatgtagagt tatttgcact
    41941 gttgtgttgg tatatctctt aatgctgtgt ctttttgggg agattaatta gtggagtgat
    42001 tatatgagta tagatgattg tttagaagaa caaataagat tatttttaaa gtttttaaaa
    42061 gttaaagttc aaaataacta cttcacatgt tatagttatg cacaccctgt tttaaatagt
    42121 cttcctttta attctttagt gcaaggattg attacaaata ttgttttatt agtctgtaca
    42181 ccaaatgaaa tatttgtaaa aaagataggc agtggtattt cttcagtaag attaaaagag
    42241 aaagaatata ctcaaaagat tattcatatt cctaagcctg aaattaagcg ttttgagata
    42301 aaagactatt ctgctttagg gctgttctca attggttact ggtgataaaa aataagaata
    42361 ctaaaactat acgtagaata taagtaatcg gcatcaatcc taattatgtg attgaatgcc
    42421 gatttttgtt acgcgctatt tttaattatc tgtaagaaga ataattttat tttactcata
    42481 ttcgttttca ctttgaagaa gtaaattgca atttcttata agttaagcag ctatgcaaaa
    42541 ggctatttat tctattgtga aatataagat gtagttgaaa caggatatat agaatcaaaa
    42601 tacgaatctt attttgattt ttctagtttt ttgaaaaaag caccctttga tcgttgtatt
    42661 agtggaaaag agaaaaagga gcatacttat catgtcacaa aactacaact aaatatctaa
    42721 acaacaccaa tttgatgcct attgaaaaaa agtattgcga aatgaagcca agtctattag
    42781 aaaatgaaat aatagaataa aagaaaatgg gaaaccgctg aatgatgtaa atgaaggtaa
    42841 gtatccacga actaattttg atgaacaaga tatctatttt ttatttgtat tggagatact
    42901 agtttaatat caaaagttat cagcagtcat aattagttat ccaatgtaaa aagtacaaac
    42961 aatagttgtt atgcagaata gtttatgaaa tatattacat aataagaaaa caagcgatat
    43021 ttttctgatc agaacaacca tttaatttaa aaagagttaa aagttatata tttttgaaat
    43081 attttggatg agtagctaaa acaatatttt gctcattaag cattaaatga agatgcaacg
    43141 atgttagaaa gttagcttct tctggatctt gttttgttaa tgcctcaaat agttgataat
    43201 gttgttccat aattttttcc cactttaatc cttccgtttt tactcgtagc caacgaaatc
    43261 gttctaagtg tgtattgtta ttttctaacc attgccatac ttcttctcgc tctgcaattt
    43321 caaaacaagc tttatggaat aaattatcat tatgaaaaaa actacgagca tttttttgac
    43381 taacagcttt ttcttgttct gaaattatag tttctaaaat ccgttttttt tggttattca
    43441 ttttggcaca acactctaac ataatttgtc gttccaaatg ttctctgaca aatcgagcat
    43501 ttttggccga atctaaatca attaatgaga tataatttcc gctttgagga attgtatcaa
    43561 caagaccttg ttgtcggagt tgaatcagtg cttcacgtat tggtgtacga ccaatagaaa
    43621 gtgtttcttc taattttttt tctgatactt tttttccagg ttctaagtca gcatagataa
    43681 tttgtcggcg aatagattgg taagcttgat tttggagact agttttaatt gtcatacaca
    43741 atgctcctta acttttattt taactgatat tttaattctg ttcatgccat ataatatcaa
    43801 attttgaagt aatacactag ttataaaaat aaaagaatac atgaaaaaaa gaaaaaataa
    43861 gtttcgataa aatcagtcca aaaagaagga gtattttatt tttctgaata aaaaatacaa
    43921 tcattctgat atggtagaaa aaagcaagtt tattcccttt ttatattgac aacggttaca
    43981 acgtgtttta aaatataaaa tgtatcaaaa ctaacatatc agtttgagga ggttaattat
    44041 ggtttcgatt aaaaattggc aaaattctat cgaagaattc aatgaaatgc agattctact
    44101 gccgtgtttt gatgtcaaag aagttaagca agaaggccag caacatccag tatggcttca
    44161 ttttggtgga ggaaatcttt atcgaggatt tcatgcagaa attgctcaat gcttagcaga
    44221 tcaaaatgaa ttagaaacag gagtagttgt gtgtgaaaca tttgatgaag aagtagtgga
    44281 aaaagcttat catgactata acaatgacat tttggaaatt gttatgagtg gagatggtga
    44341 actcaaaaaa agacttttgg cagtaacggc tgatagttta tattgcaatc cgaagaatat
    44401 agcggactat caaagagtgg ttaaatattt tgaagatcct aaattacagt ttacaacgtt
    44461 tactattact gaaaaaggtt acagtttaaa agattctaaa gggcaattct tttcggtagt
    44521 caaagctgat atcaaatctg gacctagcaa tcctcaacat acgatgagta ttgtaacatc
    44581 cttgttatat agcagattta atgctggaga gttgccgatt gcgatggttt ctactgataa
    44641 tttctcacag aatggtcaac gttttcaaga tagtattttt acgattgcag aagcttggca
    44701 aaaagaagga tatgtttctg aaaaatttat tgcttattta aaggataaag aaaaagtgac
    44761 ttttccttgg agtatgattg atcgaattac gccaaatcct tcagagaatg taaaggaaga
    44821 tctattgatg atggggctcg aggatttaga tatcattcac acaaaaaaac ataccaatat
    44881 tgctgctttc actaatactg aggtagttca ttatttaatt attgaagata gttttccaaa
    44941 tggccggcct aaattggaaa aggctggtgt gattatgaca gatagagaga cggttgataa
    45001 agcagacatc atgaaggtta cgacaagctt gaatccactt catactgctt tggcagtaac
    45061 aggttgtctg ctaggatatc aatcaatttc agcagagatg caggatgatg atttggttgg
    45121 tttaatcaaa ggaataggtt atttagaagg tttaccagta gttgaaaatc caggcattat
    45181 tgatccaaaa caatttattg atgaagtaat taataaacgt ttgcctaatc cctatattcc
    45241 agatacacca caacgaattg caacagatac ttctcaaaaa gtagcgattc gctatggtga
    45301 aacaattaaa aagtatgtcg aaaaagacgg aaatgcagaa aatctaacat ttattcctat
    45361 cgctattgct gcttggttac gttatttact ggcagtcgat gatcaagggg aaaattttgt
    45421 gccaagtcct gatccgttat tggtagagtt gcaggataaa ttatcagtat tgaaattagg
    45481 tgaacaagat ccttcaaaga ttcattgtgc ggtacaggaa attttagaaa ataaagaaat
    45541 ctttggttta gatttatatc aagttggggt aggaaagaaa a
//
LOCUS       DACEDM010000010        44289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEDM010000010 DACEDM010000000
VERSION     DACEDM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.010"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..928)
                     /locus_tag="IWO50_000561"
     CDS             complement(<1..928)
                     /locus_tag="IWO50_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357704.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESST"
     gene            complement(1183..1692)
                     /gene="mreD"
                     /locus_tag="IWO50_000562"
     CDS             complement(1183..1692)
                     /gene="mreD"
                     /locus_tag="IWO50_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0357705.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1696..2550)
                     /gene="mreC"
                     /locus_tag="IWO50_000563"
     CDS             complement(1696..2550)
                     /gene="mreC"
                     /locus_tag="IWO50_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0357706.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2705..3343)
                     /locus_tag="IWO50_000564"
     CDS             complement(2705..3343)
                     /locus_tag="IWO50_000564"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0357707.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3344..3994)
                     /locus_tag="IWO50_000565"
     CDS             complement(3344..3994)
                     /locus_tag="IWO50_000565"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0357708.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4007..4906)
                     /gene="rsgA"
                     /locus_tag="IWO50_000566"
     CDS             complement(4007..4906)
                     /gene="rsgA"
                     /locus_tag="IWO50_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0357709.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5071..7140)
                     /gene="pknB"
                     /locus_tag="IWO50_000567"
     CDS             complement(5071..7140)
                     /gene="pknB"
                     /locus_tag="IWO50_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0357710.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7137..7877)
                     /locus_tag="IWO50_000568"
     CDS             complement(7137..7877)
                     /locus_tag="IWO50_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0357711.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7890..9248)
                     /gene="rsmB"
                     /locus_tag="IWO50_000569"
     CDS             complement(7890..9248)
                     /gene="rsmB"
                     /locus_tag="IWO50_000569"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0357712.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9248..10186)
                     /locus_tag="IWO50_000570"
     CDS             complement(9248..10186)
                     /locus_tag="IWO50_000570"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0357713.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10179..10670)
                     /gene="def"
                     /locus_tag="IWO50_000571"
     CDS             complement(10179..10670)
                     /gene="def"
                     /locus_tag="IWO50_000571"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0357714.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10685..13093)
                     /gene="priA"
                     /locus_tag="IWO50_000572"
     CDS             complement(10685..13093)
                     /gene="priA"
                     /locus_tag="IWO50_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0357715.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13346..13651)
                     /locus_tag="IWO50_000573"
     CDS             complement(13346..13651)
                     /locus_tag="IWO50_000573"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0357716.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13656..14270)
                     /gene="gmk"
                     /locus_tag="IWO50_000574"
     CDS             complement(13656..14270)
                     /gene="gmk"
                     /locus_tag="IWO50_000574"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0357717.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14757..15041)
                     /locus_tag="IWO50_000575"
     CDS             complement(14757..15041)
                     /locus_tag="IWO50_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357718.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15059..15949)
                     /locus_tag="IWO50_000576"
     CDS             complement(15059..15949)
                     /locus_tag="IWO50_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0357719.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(15969..16187)
                     /locus_tag="IWO50_000577"
     CDS             complement(15969..16187)
                     /locus_tag="IWO50_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357720.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16157..17857)
                     /locus_tag="IWO50_000578"
     CDS             complement(16157..17857)
                     /locus_tag="IWO50_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0357721.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18124..18897
                     /locus_tag="IWO50_000579"
     CDS             18124..18897
                     /locus_tag="IWO50_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357722.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(18978..19547)
                     /gene="thiT"
                     /locus_tag="IWO50_000580"
     CDS             complement(18978..19547)
                     /gene="thiT"
                     /locus_tag="IWO50_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0357723.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19613..19715)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19721..20692)
                     /locus_tag="IWO50_000581"
     CDS             complement(19721..20692)
                     /locus_tag="IWO50_000581"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0357724.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20786..22159)
                     /gene="glmU"
                     /locus_tag="IWO50_000582"
     CDS             complement(20786..22159)
                     /gene="glmU"
                     /locus_tag="IWO50_000582"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0357725.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22662..23480)
                     /gene="purR"
                     /locus_tag="IWO50_000583"
     CDS             complement(22662..23480)
                     /gene="purR"
                     /locus_tag="IWO50_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0357726.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23647..24471)
                     /locus_tag="IWO50_000584"
     CDS             complement(23647..24471)
                     /locus_tag="IWO50_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0357727.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24444..25124)
                     /locus_tag="IWO50_000585"
     CDS             complement(24444..25124)
                     /locus_tag="IWO50_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357728.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25147..26085)
                     /locus_tag="IWO50_000586"
     CDS             complement(25147..26085)
                     /locus_tag="IWO50_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0357729.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26330..27181)
                     /locus_tag="IWO50_000587"
     CDS             complement(26330..27181)
                     /locus_tag="IWO50_000587"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0357730.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27335..29908)
                     /gene="mprF"
                     /locus_tag="IWO50_000588"
     CDS             complement(27335..29908)
                     /gene="mprF"
                     /locus_tag="IWO50_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0357731.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30049..30855
                     /gene="proC"
                     /locus_tag="IWO50_000589"
     CDS             30049..30855
                     /gene="proC"
                     /locus_tag="IWO50_000589"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0357732.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30934..32490)
                     /gene="rny"
                     /locus_tag="IWO50_000590"
     CDS             complement(30934..32490)
                     /gene="rny"
                     /locus_tag="IWO50_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0357733.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32901..33950)
                     /gene="recA"
                     /locus_tag="IWO50_000591"
     CDS             complement(32901..33950)
                     /gene="recA"
                     /locus_tag="IWO50_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0357734.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34059..35300)
                     /locus_tag="IWO50_000592"
     CDS             complement(34059..35300)
                     /locus_tag="IWO50_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0357735.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35540..36118)
                     /gene="pgsA"
                     /locus_tag="IWO50_000593"
     CDS             complement(35540..36118)
                     /gene="pgsA"
                     /locus_tag="IWO50_000593"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0357736.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36327..37211)
                     /locus_tag="IWO50_000594"
     CDS             complement(36327..37211)
                     /locus_tag="IWO50_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0357737.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(37989..39275)
                     /locus_tag="IWO50_000595"
     CDS             complement(37989..39275)
                     /locus_tag="IWO50_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0357738.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39268..40536)
                     /locus_tag="IWO50_000596"
     CDS             complement(39268..40536)
                     /locus_tag="IWO50_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0357739.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40788..41249
                     /gene="mscL"
                     /locus_tag="IWO50_000597"
     CDS             40788..41249
                     /gene="mscL"
                     /locus_tag="IWO50_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0357740.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41308..41754)
                     /locus_tag="IWO50_000598"
     CDS             complement(41308..41754)
                     /locus_tag="IWO50_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0357741.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41847..42092)
                     /locus_tag="IWO50_000599"
     CDS             complement(41847..42092)
                     /locus_tag="IWO50_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357742.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42207..42785)
                     /locus_tag="IWO50_000600"
     CDS             complement(42207..42785)
                     /locus_tag="IWO50_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0357743.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42862..43392)
                     /locus_tag="IWO50_000601"
     CDS             complement(42862..43392)
                     /locus_tag="IWO50_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0357744.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43396..44256)
                     /gene="rlmB"
                     /locus_tag="IWO50_000602"
     CDS             complement(43396..44256)
                     /gene="rlmB"
                     /locus_tag="IWO50_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0357745.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
BASE COUNT        12209 a         9573 c         7837 g        14670 t
ORIGIN      
        1 ctgttgaaga ttcaggtgtt gttgattctt ctgttgctga agattgtgta gctgatgatg
       61 attcttcagt cattgaagat tgtgtagctg atgattcttc tgttgctgag ctttgtgcag
      121 tagatgaagc ttgagtagct tgtgcttcag cctctgcttg ttcgcgtgct tctttttcag
      181 ctttttcttg tgcagcttgt tgacgagctt gttctgctag acgttgttgt tcacgaatac
      241 gagcttgctc tgcttcagct tctgcttttt gacggttcaa gtcagctttc ttgtcttcag
      301 cagttgcttg ttcagcagct aatgaagttt tcagtacgtt caaatcagct tgtttagata
      361 aaagatcgcc tttttgagat tccaaagcag cttggttttc agctaattct tttagtttag
      421 catcgttttc agcttttttg tcttctacag cttttttatc ttgtttttgt tgttccatca
      481 agtcgttgtt tgcttttacc atcgttgtca ttgcttgaac gcgtccaata gcatctgcta
      541 atgaatcagc attcaataca gcatcgatgt agtttgagct tgtgttgctt acttgtgctt
      601 cgcgtgcttg tttttggatt gtatcttcac gtttttcaat acgttcttgt aaatctgcaa
      661 tgtcttttac taattgtgca gattcttgac gcaatgtatc ttgttttgct aataggtctt
      721 gtgcttgtgt gttgatttca gaaacttggc tttctaaggc atcgatttgt gattgtgcat
      781 ctgcttgttg atttttcaag tctgcaatct tttgatcttg ctgttgtatc tgagaatcaa
      841 aatcgtccgc cgcggcagcg atcggtgatg ctacggcagt caaggtcatt gaacatacca
      901 ttactgctga tattaaactc tttttcactc ttcattcctc cgactggctt aatttaataa
      961 atatctaaat atatttaagt ctttcttaac gatataaaaa ttgtaccata gcagtgtgtc
     1021 aacgatataa tagttatgtt acaaaaatgt ttcaaaacaa cttttaaaaa tgacaaaact
     1081 gttcgtttca gaaaatgatc atgaaaaagc cgcgttaagt ctagcatttc ttaagataac
     1141 aacgattctt tttcgctttc gacctaaaaa taaaaaaaga aattattcat cactgaataa
     1201 tttcttaaag ggaaaaatga ataatacaaa tataatcatg ttcaacagca gtgtaggtcc
     1261 taaaaacctt gtaataaaat acgtgttatt tactactgct aatttaaaaa tcaactggac
     1321 caccatcgta aacaattcat aacccgtgac aaagatgatc atactgaaaa attcagtaaa
     1381 gatgttgaca tggataacgt ctttcattac atacatcaac catacaatta aagggagagc
     1441 tactgcatag ataccgataa cgccaatata gtaagcgtca tagatagccc cgagaacaat
     1501 cgtggtaatc agtaaataac gtttttcaaa cgccatactg caacataata atgccaatat
     1561 cagaaagtgg gcatttgaca tataggtgcc tttcgaccac tcccctagca ttcttgttaa
     1621 atgaccatct atcaacatca aaaagaacaa aacgattggc agatagtact tcattgtttc
     1681 tttcttcaac atggactaat cctccgctag ccgttggaca atcgtcacaa ctgacacgtc
     1741 atacatttct gcataaggtt tcacataaac ttcccgatcc agcccataac tatctggttt
     1801 cgttttaata accgtaccga tcggcaagtt agctggagaa ttccctccaa gaccggatgt
     1861 ctggacaaca tccccttctt tgatatccgt atcaccagtt aattgggtca ccactaaggc
     1921 atggagcttt tcatcatagt ttttaagcaa accaaacgct tcgccattag ccgaagatac
     1981 ccgtactgga aaatgattgg agctttcatt agaggatgac agtaattcga ttttagacga
     2041 agccgtattg acctcgatca ctcggccaat caatcctttt tgcgacatga ctgccatatt
     2101 tgcttcgata ccgtctttag aacccttgtc tacgatcagc atgtcctgcc aactgtcagg
     2161 cgaacgagta atgacatttg ccgttacttt ttcgtaacta gtcagcgtct gattcaactc
     2221 aagttctttt ttcaacgctt cgatttctct ctcgtaattc ttgttttgct gtgaaacgtc
     2281 atcatagcta tcgatttttt cttttagccg ctcattttcg gcataagttg aaaaaagatt
     2341 atggacagaa tctactccat ttcgtaccca ccgtactggg gcagagattg ttttatcaac
     2401 cattgcaaca ctgtcattaa cgatggattg gaagaaattg ggctttcctt cattcgctcg
     2461 ctgcgcagcg gtcacactta gtatcgtcac cacgatgata acaataacca aggtaataat
     2521 aatattttta ttaggattga acttcttcac aaagacacct cgaattaatt agactcttgt
     2581 gttcaatttt atcactaaat gccgtgaaac aaaaggtaag ttgctcctct taagcttttt
     2641 tttagcaaat aatagattct gtcattttta gcctgaaaga caaatttttt tatttgttta
     2701 tggatcaatc tttgctttga ataactgcaa tcataccatc tgaaaaagaa aattctgcgg
     2761 tatctgaaag aaattcgtta cttgcataag atgtcggata ttctacttct ttattttcca
     2821 atacatactt gctgcctttt aatgtaagat tcgagacggg agtcaagcag caataagcta
     2881 aatacttcat atctggtatc tgtcttacta tatgctctcc aggcaaataa taagacaaat
     2941 aattttgaca atctctgata ataagctgat gggcaaaggg tttgaagcga ggttccatac
     3001 ctaaccagag attggctaaa agatgatcca atcttccgcc cgtagctcca atcaaagtga
     3061 cttccgcttg cgggtaacgt tcaaatgttt ttaagatacc taactgtgta tctgtatcgt
     3121 ctttttctgc aggggaacgg gtatactctt tggcttttgc aaatacttcc ttctgctctt
     3181 cgttcgacaa tgaatcaaaa tcacccactg ccagatctac tgggtagcct gcttgaagca
     3241 ggtacagcgc accacggtct atcccgacat aggcatcgta tttggacagc agagactgcg
     3301 gccagttttg cggattacct ccagcagcta ataaaatttt catttagtct aatgcctctt
     3361 ttaatcgttc gatttgtccg actggatttt cggctccata gatatatgat cctgcaacga
     3421 agacatccgc ccctgcatct ttacagattt ttgctgtttc tggaacgatt ccgccatcta
     3481 cttcgattgc ataagtccaa ttgttctctc tacgtagatc agcgagttcc gtaatttttt
     3541 ctactgtttc ttcaataaag ctttgtccgc cgaacccagg attgacagtc atgactagta
     3601 cttgatcagc caaaggcaaa acatgtttga tcatggagac tggtgttcct ggattgataa
     3661 ctactccagc tttaactcct aagctttttg tcatttgaag tgcccgatga atatgaggag
     3721 ttgcttcgac gtggacagta atgatatctg ctccagcttt tgcaaatgct ggaatgtagt
     3781 tttctggatc aacgatcatc agatggacgt ctaacggtaa tttagtaaca ggacggattg
     3841 cctgaacaat atttggccct aatgtgatat ttgggacaaa tcggccatcc attacatcta
     3901 cgtggatgta atctgcgcct cctttttcca ctaactcaat gtctctttgt aaattggcaa
     3961 aatctgcact caaaatggat ggtgctatct tcattcttgt ttcctcctat tttcgtttac
     4021 cttctttttt tgcatacatt ggttttcttt tttcgatttc ttgtaagaat tgcaaataat
     4081 tgtcgtaacg tgtctgtgcg ataccacctg tttctactct atgttttact tcgcagccag
     4141 gttctttagc atgcatgcac tctctaaatt tgcaatggac agaagcttct acaaattcag
     4201 gaaattgtct cggcagttct cctgcttcaa ttgtcaaaaa gtcaatcgag ctaaatcctg
     4261 gtgtatctgc tactaacccg ccatagatag gaatcagttc cacatgacgt gttgtatgcc
     4321 gtcctcttcc aagtgcatca gaaatctctc ctgttgctaa attcagttcc ggtgatattt
     4381 gattcagtag cgtcgatttt cctgctccag actgacccat aaagaccgat agccgttctg
     4441 ggaagtaacg gatcagttct gtcgtatcac ccgtatgatt gggtacaatc accggatagc
     4501 ctattttagt ataggtttgc ttgatctctt cgattttttc tgggtcgtct gacaagtcgg
     4561 tctttgtcaa aaaaataatc ggttcgatcg actcgtattc caaggtaact agaaagcggt
     4621 caagtagatt ataagaaaat gcaggctcta cgatacttgt gacaatgatt ccaagatcaa
     4681 catttgtgac tggtgggcga actaattgat tttttctcgg caagatttcc agaagatagc
     4741 cgtctgtttg attttcactc tcaaagatca cctgatctcc gacaagcggc gtgattttcc
     4801 gattccggaa atttcctctt cctcttgttt gataggtctc tccgtttgcg tatacatagt
     4861 aaaaaccgct gattgctttt ctgatttgtc ctttgataag tgccacctcc gtttttgttt
     4921 attttaccat acgaagagga aagtaggtgt tattccatgc aaacttctca aagtatatgg
     4981 cttgaaaatt gatcaaaaga gatagaaaca caaaaaagag aatgcaacaa atcaatgtcg
     5041 cattcccttc tgatatattt tattcagcac ttattttgaa ctttctgagg tgctagctga
     5101 tgaacttgtg gagctggaag attcttcttt tgaagagttg cttgtgctac tggagctact
     5161 tgagctgcta gaactgtctg aagagtcttt ttcagaacta gaagacgctg gatctggtcc
     5221 tttagagtaa attacgttca caaccattcc tttggtaatt gtcgttcctg cccctggtac
     5281 tgtacgaatc actttatctt tatccaccgt gtttgaatat tcggaagtct catgcccgat
     5341 atattctgca ccgacactag ctaaataact ttgtgcttcg ctttttgtat agccggagat
     5401 atcattcaat ttaggctctg ttccttggct tacgacaaaa ttgatgtaag tttcttctgc
     5461 tacaacttca cccgaggctg gatcctgact gatgatcgaa cctgatggta cagtgtcgct
     5521 atagtcataa gtagcactaa tattattgat attgaatcct tgtgtaacaa gccaattacg
     5581 aacagtttcg tagttttctc ctacatgact actcaaagtc actgtttctg gtcctttgct
     5641 gactgtgaaa gtaatcgtgt ctgttttagg atcgacttct gtatcttctg atggagattg
     5701 cgaaatgatc attccgcttt ctacctctga actgaattcc tcttcttttt gaatcaagtc
     5761 agcagaaaac cctaattctt tcaaacttgc ttcggcatct tcataacttt taccactgta
     5821 atcatctaat ttgatttttt tcgtgccgct actaatatac agagtgactt cgcgtttttg
     5881 cttgactgtc gttcccgctg ccgggtctgt cttcactaca ttaccttctt cgattttgtc
     5941 atctgcaatt tcttctgttt ttgttgctac ttctaatttc gcttttgcga gtgtttctct
     6001 agcagaagct tcagaaagat cagaaacatc aggaacaaca acttctcctc gcccgccaga
     6061 agcaaagaat acagctgttc cgattcctaa aacagcaaga atagctaata ggatcagcca
     6121 ccattttttc tgtcctttct ttttaggatc aggcagaact tcttcctcct ctggtaaggt
     6181 ttcgttttct ggcttgtctt ttggcagagg catagatttg aatgaatcag gcattggtgc
     6241 atcttccgta aggggtgtga taacttttgt ttcattatcc atcacatgag gctgccattt
     6301 ttcttcgttt gcacgagctg gtgataatac tgtagaaaga tcatcagaca tttcctcggc
     6361 tgacttataa cggtctgcag gttctttagc cgttgcccgt aaaatcacgt tttccagtgc
     6421 ttgtggcaca tttggatcaa gtgcttttat agatggaaga tcatcttgaa aatgttttaa
     6481 agcaattgtt acagcagatt caccatcaaa cggtacgcta cccgtcaaca tctcataaag
     6541 gatgatccct aaagcataaa tatctgactg cttcgttgcc atacttcctc tggcctgctc
     6601 aggggacaga taatgaacag atcctagcat cgtattcgtc tgcgtgatcg atgtttcaga
     6661 taaagcgata gcaatcccga aatccgtgat ttttacaaca ccttcattat cgatcagtac
     6721 gttttggggt ttcaaatctc ggtggatgat ttgatggtta tgagctaaag aaatagcaga
     6781 aaggatctgc tgcataatat ttactgctgt ttcataagga acaggataat gtgtttggat
     6841 atatcgtttc aagtccattc ctttgacgta ttccatcacc agatactgca tgttgtcttc
     6901 ttcaccaaca tcgtaaacac taacaatgtt tggatgaacg agttctgttg ctgctaaagc
     6961 ttctctctga aatcgtcgaa tcgcagtctg gtcattttga aagtcaaatc gtaagacttt
     7021 aactgctaca tcacgatcca agattaaatc gtgcgccaaa aatacatttg ccataccacc
     7081 gctaccgata tttccagtga tttgataacg gccatttaat tttttgccca actcgatcat
     7141 acactgtcct ccttatattc gataagaaga acggtgatat tatccgctcc gcctgcttca
     7201 ttggcacgat caatcagctc agttacttta tgagacaatg ggccttcttg attgatgatc
     7261 gttcctattt cttcttcaga cagcatgttc gtcaagccat ctgaacataa cagcagtcga
     7321 tcttttagtt gccaaagatg agtagaaaca tctacttcga ctgttccagg catgccgaca
     7381 gaacgtgtta aaatattttt ccttggatgg ttcactgcca tttcttcgct gatttctcct
     7441 gatttgacta gttcattgac taaagaatga tcctccgtca attgcatgat tttatcttca
     7501 cgaatcaaat aagctctgct atccccaaca ttggcaatcg tgaaccagtc atctgacaga
     7561 gcggccgcta cgattgttgt tcccataccg ttatattccg gctgttcttg tccacgctga
     7621 taaatcagca tattttcttc ttgaatcgat tgaatgaacc attgggcgat tttttcatcg
     7681 tcaacagctt cttgtttttc ccaagcttcg cctagattcg ttaccgccat ctgactggca
     7741 atgtctccgg cacgatgacc acccatcccg tctgctaata cagcaagctt gagacctgaa
     7801 cggctggtaa atacactggc atagtcttga tttgtgttac gtcgtcttcc tacatctgat
     7861 tgatattcaa tctgcatttt ttcacctcat tactttttgc gcaaacaaca aatgaaaaat
     7921 ccatctgtgt aaaactgatg cggatacaaa gtcaacattt tctcttcgat tgatgattgt
     7981 agcataggat tggtcaaaac atcaatcctc tcaaaatcag ggtgtctttc caagaaagca
     8041 gaaacaacgt cttggttttc ttcttttaag atcgtacaag tgctataagt cagaataccg
     8101 gaagacttta aagttggtgc gcagctttct aaaatttcca attggatttt tggtaaattc
     8161 gctaattcat ccggctgttt tttataacga atatctggtt ttcgccgaag cagcccaaga
     8221 cctgaacaag gcgcgtctac taaaatccga tcaaactgct ctgcttcgaa ctgtttactg
     8281 acttctctag catccatctt ttgagcgtag acagcagaag agacacctag acgtgctgca
     8341 ttatcttcaa tgagtttgac tttgtgttcg tgtacatcta aagcgaccac ttctccaccg
     8401 catgacggat ctaaaaaaga agcaatatgc gtcgtcttac ctccgggtgc tgcacaggca
     8461 tctaagacat gatgttcagg ctcgatttgc atagacggcg ctacaagcat cgaactttca
     8521 tcttggatcg tcatgacgcc atcgtggaac aatgagctag aagctaaatg cccttttttc
     8581 gctactatac cgtatggact gacttggctt ttttcagctg tgatctcttc ttcttgcaaa
     8641 acagtgatgg cctgatctct ggaaagtcgg cgtgtatcta ctcgggcact agcatggcta
     8701 ggctgatata gcgataaacc gagcttttca gtttcttcgt atccgatttg atccaccagt
     8761 cgtttcgtca tccatagagg caggctgatt ttgacagaaa gtctttcgat aggatcagca
     8821 atgccttgca gatctggtgc tccttgacgc tgataattcc tcaagacacc atttacaaat
     8881 ttccctgtcc caggatttcc ttttactttt gcgatttcga ccgcctcgtt taaaatagca
     8941 tgtgcaggga ctttatctaa aaattctaat tggtatacag aaagataaag aagcatcttg
     9001 acccagtcat ccactttttt tgctttagcg atcaatggtg caacgtaaaa ttccaatagt
     9061 cgctgatggc taatcgtacc atagatcaat tctgtaaata aacccacatc tttttgattt
     9121 agctcgcttt gtttgatcat ctcattcaac aaaagattcg agtaggcgcc acccttgttc
     9181 actcgctcta atgtcagtaa tgccatatat cgtactgatt ttttcatttt tttagggatt
     9241 tttttcatta tttcacctgc tgaccaatct gaacttgctg accgcttccg ttaaggaaat
     9301 catgtacggc ctgttttcct ttaccagctg gctgtaattc attgatggct aagacggtct
     9361 gtttaccgca agcaatccat agattttttt tgtctttccg gatgatcgta ccaggtgctt
     9421 tgtctgtagt ttctgccaat acttcaaccg ataataattt ccagcgtatg ccctcatagg
     9481 tcgtgaatgc aattggccaa ggacgcattc cgcgtacttg gttgtcaatt tcttctgcag
     9541 ttttatgcca atcgattgct tcttgttccc gtgtgatgtt tggcgaaaaa gttgcttctg
     9601 actcgtcttg aggtactggt ttttgtccat ccaaaatatt gggcaatgtt tctagcaata
     9661 gctttcttcc taaaaggcta agcttttcaa acatcgtccc tacatcatct tgtttagtga
     9721 ttggtatgct ttcttgcgca tagattccgc ctgcatccat ttttttgatc atttccatga
     9781 tcgttacacc agtttctttc tccccattca tgatggcata atgaacaggc gcacctcccc
     9841 gatatttagg aagtaaagaa gcatggacat tgattgcccc taattttggt acttgcaata
     9901 acttttcagg caagaattgg ccaaatgccg cagtgacgat cacatcaggc accaattcaa
     9961 tgatttcttc catttctggc gaaccggaga ttttctctgg ctgcagcaca cggataccgt
    10021 gcttcagagc cgcttttttt accggtgtcg gcgtaattac tcgtttccgc ccaactggac
    10081 ggtcgggctg tgttaccact gcctggatct cgtaacctga ttctatcagc ccttcaagaa
    10141 tcggcacaga aaaagcaggt gtgcccataa atactagctt agtcatcaag atgttcctcc
    10201 atatattctt caagttcttc ttccgggatc tgctgtatca ttttctcaat gaacaagata
    10261 ccgtctaagt gatcgatttc atgctggaag gcacgggcta aatagccaaa agccgtcact
    10321 tctatctctt ctccgtctcg atcataataa cgcacggtca cttcatctgc tcgcttgacc
    10381 gttccataga catgcggaat actgagacaa ccttctacgt caaggctttc accttttgct
    10441 tcgatgattt ctgggttaat cagttcaaat ttctcgcctt catctacttc gatcacagca
    10501 attctcttgt tttgtcctac ttgaggagca gcgatcccta ttccgtcatt cgcaatcatt
    10561 gtctcatata aattgtctaa taaagcgatt gtctcgtccg tgattacatc gattggctga
    10621 gcagttcttt ttaatttatc attagggtgg atcaatatag gatagcgcat aatttggccc
    10681 tttcttaaat aaagtttaat ggttcgttgt caattgccac aaaaagacct tgtcgctgtt
    10741 ctttctggct tccattcaaa atttcatcca aaacatgagg aagattcggt tcatgtttat
    10801 atttgatgat tagttgataa taatatcggt ttttcactct caaaatcatg ctgggcgttg
    10861 gccctaatag caagctttga gggcttaaca cttcttttag ctgattggca atctgaaaga
    10921 ttttctttgc cgctacttgt tcttcaggat ggctggcggt tattttcaca gtaaagtaat
    10981 aaggaggata gcctccacga tgccgtacat gcatttcctg ctgataaaac tgctcataat
    11041 tttgtttttt agccaattct atggcgtaat gttgaggatt gaatgtttgg atcacgactt
    11101 cgcctgcttt ttccgctcgc cctgctcgtc ctgaaacttg ggtaagcaat tggaatgtat
    11161 gctcactaga acgaaaatcc ggtaaattca gggaggtatc agcattcaac accccaacta
    11221 atgtcacttc tggaaagtcc aaacctttag caatcatttg tgtccctaat aatatatctg
    11281 cttcttttgc accgaatttt tgtaaaatct gttcgtgtgc cccttttctt cgagtggtat
    11341 ctacatccat acgcaaaatc ctagcttgcg gataaagttc tcggagttcc tcttctactt
    11401 tttgagtacc tgttccatag tagcggattt tattgccgcc acaattagga cagcgattgg
    11461 gaataggttc ttcatgtccg caataatggc agcgcattga acgtgtatcc atatgcagtg
    11521 taagagagat atcgcaatta ggacatggaa gaacaaatcc acagtcccta cacatcacaa
    11581 aagacgaata tcctcgacga ttgagcagta ggacagtttg ttcttttttg tctaatcgat
    11641 tttgtatttt ttcctgcaaa ttagcagaaa aagtagaggt ccgatgattc tgaaactctt
    11701 cacgcatatc tacgatctca atagctggaa gctgtgcagc tgctttggct cggtggttta
    11761 actgcaataa ctgataaacg cctttttgag ctcgtgctct tgattctaaa gagggcgtcg
    11821 cgcttcctag aactattggg caatgatgat atttactgcg ccaaattgct agatctctcg
    11881 catgataacg cggtgtctca tcttgtttgt aacttgattc atgttcttca tcgataatga
    11941 tcagcccgat atttttcagc ggggcaaaaa tagcagatcg tgcgcctact actacttgcg
    12001 cttcttcccg ctcaatcttg cgccattcat catatttttc accttgggaa agtccgctat
    12061 gtaagaccgc aactgattct ccgaagcgtc ctttgaatcg ctcgaccatt tgcggagtca
    12121 atgcgatttc aggcactaac ataatagccg ttttattttc tgacaaaaca tcagcgattg
    12181 cctgaagata tacttctgtt ttcccgctgc cagtgattcc ctccagcaaa aagacctgat
    12241 ctttttgctg ctgacctgct gtgatgattt gttcgacagc atttttttgt tctgcattca
    12301 gttccaatgc cgtcgtttgg tcaaatactc ggtctttata agggtcgcga taacgttctg
    12361 attctataaa ctccagccag cccttttctt ttccttgatt caagacagca gtactgattc
    12421 ctttattttt gaaataagca attggttctt cttggctcag ttcagataaa cacaaaagaa
    12481 gttctttttg tttcttggca ttttgccgta attttgccca ttcttcttcg atttgctttt
    12541 cagttaacaa agaacggacc aaacgaatcg tttttacttt atttcgtgta tggacttcat
    12601 atttgatatc tactaattgc tcttggcgcc agcgaatcaa ttgagaaagg ctgccttctt
    12661 cttctgcttc cttccaggaa atcacttctc gctgatcaaa caatacctga acttctggtg
    12721 tgtctgccaa aggatagatc aatttatcat attctgcttt catcacactc ggaagcatcg
    12781 tttgtagaca agtgattttg aatgcgtaag taattttttt catataatca gctaatgaaa
    12841 gtaattcttc attgaccacc ggagcaaggt ctagcaaacg aataactgcc ttcaaagatg
    12901 gattagctga ttcttctgat ttttggatag ccataacaaa tccttggaca tgacgattgc
    12961 cattaccaaa agggacttct actcgcatcc cgacttgcaa agctgtttcc atttcagagg
    13021 gaaccaaata agtgaatggc tgatcggtct gcatagtagg tacgtccaca atgacttcag
    13081 ctgttaatgt catggttcgt ttcctccttt ttttgtcttt tcttatcaag cgacaaaaag
    13141 ttccttgcct attttactag agatcgatgg atttgtctta tcttctgcca aatcctaaca
    13201 aaaaattaac agcagataag aaaaaaaaga actgtgtcaa aaagttcaga cacagccttt
    13261 tctaccagac aataagtggc gaatgtttga aaaggaaaca gctgggtttg cttttctttt
    13321 tagatgttcg ctctattttt ctttattaca ttttttgctc ttcacggata cgtgattcca
    13381 attcgtgttg ttcacgctct ttttgtgctt gacgttcctc ttcttccatc ttcaaacgtt
    13441 cacgttttaa ttcaggatgt ggatcattga ctacatttcc tgcatcgatt tcttctaaag
    13501 cttgaccgac gctttttact gaatcaaaag aatctagagt aggctgtgca cttgcatcca
    13561 attcatgcgc gcgtttactt gctaaaatca ctagagaata ttttgaattt acacgatcta
    13621 atagtgagtc aattgaaggt tttaacatca taagactaca tctcctttaa cattttaata
    13681 tatttaccga ttacgcgatc tactcgaaaa tgttcactgg caataatgtt tttgattcgt
    13741 tcaactgcta gcggcacttc atcatttaca acagcatagt cgtaaagagc catcatttcg
    13801 atttcttctt ttgcaacttt cattcgtttt tcgattactt catctgcatc tgttcctcgg
    13861 cctacgatcc gtgattttaa ttccgctaaa tctggcggag tcaaaaagat gaatacacca
    13921 tcgggcactt tttctttgac ttgttttgct ccttggactt caatctccaa gaaaacatct
    13981 tttccttcat ccaatgtctt gttgacatat gacaaaggag ttccataata attcccgaca
    14041 tattctgcgt attccagcat ctctccagct tcgatcattg attcaaactc ttctttgctt
    14101 cggaagaaat aatcaacgcc ttcgacctcc ccttcacgca ttttacgggt agtcattgag
    14161 atcgaatact gaaaatcatt atcctcactc tcaaaaattg cttttcgtac tgtgcctttt
    14221 cctacaccgg atggaccaga caacacgatt aataatccac gctctgacat gacgacgtcc
    14281 tttcaactag tctttttgta cttatcgttt attttgcact ttttttctgc aactttcaag
    14341 gttatcttta attatagcta gaaattggta aaaaagctac cctgtaccaa gaggtcgtaa
    14401 aaaagctgtc ctgcatcaag taataagaac agccaaacat aaacagcttc tcatgttttt
    14461 cgtttggttg gggttattat cgcagatgca agcttttgaa cgccgtgtat aagaggtcgt
    14521 gaagaagccg attagtttcg agtatgaaga aaaattttgt gaaaatagtt tctcacattt
    14581 ttgcataaat ttggcttaat accgagaaaa tggtttttga acatcactta ttcaaaataa
    14641 aaaaagattg tgacacgaat tttgtcacaa cctttaaatg tattcctaga gcaaagaagc
    14701 taagcgaaga aatccaattc tgtttattta ttgtatttct ttgttcagca tccttgctaa
    14761 aagcccatat tggacaaagt attcactaca cccatgatct catcttttcg agaacgtgct
    14821 ttttctgtcg gatcttctac ctcttctgtg tcaattcgaa caataaattt ccgattcatc
    14881 gtcacgtaag caccgtcgtt gatcaaaata aacagttcgt tctcattata gtctgcacag
    14941 gcatctttcc atacgtcaaa gccgtttttt tcagaaggga tatcccgttt aaccacagtt
    15001 tttccactta attcgtggaa cgttacgtca taatatttca ttatctctac ctctttcgtc
    15061 attcaatatt ttgtacttgt tctctgattt tttctaagat cgtcttagac tggacgactt
    15121 gatttttaat ttcgattgca tttgatttgg aaccgatcgt gttgatttcc cgattcattt
    15181 cttggatcag gaaatctaat tcccgaccta caggacattg tgcttcgata agttcatcta
    15241 gtttttgtac atggatcgcc agacgatcca gttcctcgtg aatatctccg cgttccaaca
    15301 agatggctag ctccgtcaac aaccgttgct catcgacttt cgctccaaga aattcctcca
    15361 gctttttttg ataccttatt tgaaattcct tttcatagat ttctacaaaa ctatcgagtt
    15421 tttttagtac ttctttgaat tcttcagtat tctttttcaa cactaaagct aaagcttgac
    15481 cttctttttc tcgactcgcc tcaatagaat caagtgcgct tttcaatgta tctagagcta
    15541 actgtgccaa ttcttccatg ctctcttccg gtttttcttc taccgttacg aagttttcgt
    15601 ccagcacgaa gcgttccgcc atccgactaa ttgacacttg attttgacta ccgtatcgtt
    15661 gttgaattcc gtctgtcaat tgagtgataa gtgaatcaat caattcccag ttgatgacga
    15721 tctgtttttc gtttttgcca atataagcta gattcacaaa tacttcgatt cgaccgcggc
    15781 ttaactgctg tttaataagc tgacgaaaat cgttttcaac aaagttcagg attttaggac
    15841 ttcgtatttg aacatctaga aaacgctggt tgacactttt tatttcaata tctaactgat
    15901 acgcttttgt ttcgcggatg gctttgccaa aaccagtcat acttttcatt cgacagggtc
    15961 cttcctcatt acatattttc tattgctttt tgcagttgtt caaactcttc gttcgtcaat
    16021 gtcaggcctt tccccatttt ttcatggtct ggcgcccaat ctcgaagatc gaattttggc
    16081 gggcgaccat tccagctgac taggttcagt tctttacgcc accccttgtt gttttctgac
    16141 aatatagcga tttcttctac gatttcatat gaaaattctt gtgccatggt tgttcctcct
    16201 tgagataata taaatatagg atcagtaatg tgtcaactgg ttgcttaagc aattcatgct
    16261 gcaaacgatc cttgcgtttt ccttgtagca gataatcaaa ataaacagtt ttcgtgcgct
    16321 cattttgtat tttttgctgg tattttcgat accttcgcca ttcttcagta cttaattgac
    16381 caatttgtgc caataaatca tccggcatac tttttatatc ttgttggcag atcatttgaa
    16441 tcatcatgtc tacttgatag atttcattgg gcagtaaaaa atgttttttt gctaaaagtt
    16501 ctagcatcgc caatccgcat gtctctggta cgaccctgtt cttatataaa ttacgcagtt
    16561 tttgccaaaa ttttatgctt ttgaaaaaag ataattctaa ctgaatttct tttgttaagt
    16621 aagacaaaaa tcctcgccag tatgttcttg caggtttaac agaatgaaat gtataccagt
    16681 tcccatagta atagcctaat cgttttttca tttccagata gcctaattgt aaatttaatt
    16741 ggttcctttg gctatcaaaa agcagaaagc tgcctagtgt ccacaatgtt tgacatttcc
    16801 aatggataaa aatgtctggc atacgaattt ttttcgctgg gcctggccct tgcacatcta
    16861 cgacaaatgc ctctgttgct cctttgttaa tcgcaatatc aataggtatt ttgttgcgat
    16921 acccaccgtc tgcatattta ttttttccga tctttcgata agccatagcg ggataaaatg
    16981 aagcagaggc gagtatccaa tctggtattt cttctgtttt tgtctgttgg atatcagtca
    17041 ctacttccgc tagttctggc agtttagtag acaccgtata taaacgtatt ttgggattat
    17101 gtttgagtaa ggacaaatcc aaattgtctt tgaccatctt ttccaatgga gcagaagaaa
    17161 ccccgccttc aattagtgct gttttcgtca tttggcgtct ttctcgatag aagcgttcac
    17221 gaaggtcttc aactgcagcc atttctggta acgcaagcac ttgtcttgtc gacagctttt
    17281 tccataaatt cacagctttt ttcatatctc cttgtaaaat caatgcgccg ttcaacgcac
    17341 ctaccgaggt acctgtgata atctcaaagt tgatatcatg ttccttcagc gcctgccaca
    17401 caccaatctg ataagcaccg tgcgctcctc ctccacctaa aacaagtgct gtatgataga
    17461 ccagcttttt atttaattgt gtggttcctt gatagccgtt tttatgaaaa atacgcaacc
    17521 attcttctga tagaggagat tgaaataaaa aacaacagtt ttctttaata aaaaaacgag
    17581 cgcaagcttc catataccga aaaaattttt tccattcaaa agtttcacct tcaatcaaca
    17641 gattattaaa aataacatct cctctacttt tagcaatagt aaaatagacg gtatattttt
    17701 tcgatactgc tttaaaagca cattgctttt ttgcagaacg ccaaaaagcg tctctacttt
    17761 cttgatgact ttgaaaaaat ggaaggggtt gttttgcttg ctggataggc gaatacagcg
    17821 acattttctg tgaaaagtaa acttttttta tctccatcgc gctctccttt ttcttcaagt
    17881 ataccagtac gtcataaaaa ggtcatgagt ctcgtattcg actttgtaga ttccataaga
    17941 aaacaattca taaaacctcc tttaaataaa aaaatacaaa aatcacaaaa attaattgaa
    18001 aatgagagtg ttttcaatga aaacgtgatt ttgtaatgga attttaaaat aaaagaaaca
    18061 agctttttta atgcaaatgt tgtaaaataa aagagattaa gaggaaataa gaggaggaac
    18121 atgatgactg agtcagttta tgtacatatt gataccacaa gtaatgctgt tttaacaaaa
    18181 gggctgactg ctactgactt tgcaaacagt atcgtgcatt ttcctcaaaa cttgctcttg
    18241 cttgatccat ctgcttctgc tggagaatat gaatcccata cagggctgaa agtaatccgt
    18301 ggtacagaaa atattcagcg tttcttttcc tcttctcgta acaggcgtgg attcgatgaa
    18361 ttgaagtgga tcgactttac tgatttaacg atgctgaaag aattgacacc tttagaaatt
    18421 tcagaattat tatattttgg tcatatgaaa acacatcttc attcgccgtt tttttataaa
    18481 ctacagaata attttgtata cttcgatctt aatgatcagc tgaatcggat ttattatcgc
    18541 tatttagatg aattttatag gatttttgct agtaaattaa gttcgatctt gtcagataag
    18601 atcaatgtaa aaaaatcttt tttcaaaaaa gaaacacctg ttgaaaaaat cagtgtggaa
    18661 ttattgaaag aaatgcgtaa tttgatgcag gaagggatca ttttttcttt tgatcaagtt
    18721 ggtttgatcg atggtaaata tcgtatccca atctatgtga ttgaagatta tatttggaaa
    18781 ttaaaaggca cccggtatcg agaagaggaa atcatcgcta ctttaatcta ctctgtcacc
    18841 gatcgacact ggcaattggt agaagatgcc gatctttctt atctgccatt gaagtagatc
    18901 tatttcttat caaaaaataa aaaggaaaca agaaactagg acatgattct gtctcaagtt
    18961 tcttgcttct tttttattta cagctcctgt cgtttatctg gcacaaaaat cgaaggagtc
    19021 gtttgttgga tccctaataa aacgatgatc gttacaatag ctgtactcaa cccgctggca
    19081 ccattcatta cgaaagaaaa gagccaagga ttcatccccc atagtgcata cgctccccag
    19141 aaaaccactc ctgcaataaa atgccaaaaa tatctggaaa gtgcgccaat caatgcacca
    19201 aacacgatgc tttttctaat ttgtttcgta ttatcgtcta catagccttt ttggacattt
    19261 gaagccatga gccctgctgc gcctgcaaaa gtataggcaa tcgggtactc aatcaacact
    19321 tgaacgacac ttaaataata aacttgtccc aacggaaaat gtaggatgcc ccaaatgaaa
    19381 cctgccagca tccctgcctt tgtccctctt cttaacgcaa aaagagtcaa tggaatctgc
    19441 cctaatgaaa tagaaaaact gctgccgatc tggatcggaa tcaacgacaa caccatcgct
    19501 aacgctgcaa caatcgttcc ttctacccaa actctatttt ttcccataaa aaaacttcct
    19561 cctttgaata aaaaagtcac aaaggaggaa gcagctttcg aaaatagtca aacagctgtt
    19621 tattcacaat tcctacgctc gcataaccga tacaggtacg aagggtttag aaatagattc
    19681 aatctcagcc caattggact cccctttgtg agtttattta ttatttattt tttgtttcaa
    19741 acaatggact aactggtttg ttttcatgga tacgtttgat tgcatctccc attagtccgc
    19801 caacacttac ttcatcgatt ttgtcgattt tacgatcttc tggaagatag atcgagtctg
    19861 taacaactag acgctcgata gctgaatctt ctattctttg taaagcaggg ccggataata
    19921 ctggatgtgt acatgaagca tacacactag ttgcaccagc ttctttcaaa gcattggcag
    19981 ccaatgtgat cgtacctgct gtatcgatca tatcatcgat cagtacacaa gttttgcctt
    20041 tgacttgtcc aatgatattc attacttctg caacatttgc ttttggacgg cgtttgtcta
    20101 tgatcgcaat cggagatttc aaaaattccg ctaatttacg tgcccgtgtg acaccaccat
    20161 ggtctggtga aacaacgaca acatcatcgc ctttgattcc tttttctaag aaataattgg
    20221 caataagtgg cgcacccatc aaatgatcga ctggaatatc aaagaaacct tggatttgga
    20281 ctgcatgaag gtccaaagtc agcattcttg ttgcaccagc tttttgaatc atgtttgcta
    20341 ctaatttcgc tgtgattggt tcccgtgcac gtgctttacg gtcttgacgc gcatagccat
    20401 aataaggcat gactacgttg attgtttttg cacttgctcg tttcagtgcg tcaatcatga
    20461 tcagcaactc catcaagtta tcattgactg ggctgctcgt tgattgaata acatagacat
    20521 gcgatccacg aatactttct tcaatgttta cttgaatttc tccatcacta aattgagtaa
    20581 cagatgattt tcctaactcg acgccgactg ctgctgcaat tttctctgct aatgggcgat
    20641 tggaatttaa cgcaaaaatt tttaatcttg ggtcaaagta atgttttgac atggggacct
    20701 ccactttctt tttcacaact ctagaattct acctaataaa tagtagtcat attttttaaa
    20761 taaatcaagt gattttgcaa aagtcttagt ttagataagg gagttttttt gcatatcctt
    20821 ctttattgac ttgtcttgct ctcgcaattg ccagcgcata ttctggaata ttgtctgtaa
    20881 ttgttgaccc ggcagcaatc gaactgtttt tagctacctc gacaggtccg atgatattcg
    20941 tgctagaacc aataaagctg tggtcgccga cagtcgtatg atgtttgttc tttccatcat
    21001 agttgacgaa gacaacacca catccgacat tgatttcttc tcccagcgta gcatcaccaa
    21061 cataagtcaa gtgaccgact ttagttcctt tgccgatttg agcatttttc acttccacaa
    21121 agtttccgat atggacgttt tcgccaattt cagcttttgg tcgtaaatgt gcataaggtc
    21181 ccacatctgc atgtttcttg acatggctgc tttcaatgac tgagttttca atgacgacgt
    21241 gatcctcaat cctactgtct acgatttttg aatgtgaccc gatgacacaa tcagaaccaa
    21301 tgactgtatt tccttggatt tgtacacctg cttcgatcaa tgtgtctgga ccgatttcta
    21361 cgcctgcatc gatgtatgtc gttgctgaat cgacaaagct tacgccattg accatatgca
    21421 tctggttaat tcgctttctc atgatctcat ttgctttagc taacgcgatc cgatcattga
    21481 ctcccattga ttcttcaaaa tcatccgttt gataagcagc aactgtatgg ccttcttctt
    21541 ttaagatttc gatgatatct gttagatagt attcaccttg agcattgttt gttccaactt
    21601 tgcttaacgc atcgaacaaa gcttcgttgt caaaacagta agttcctgta ttgatttctt
    21661 gtacaagagc ttcttctggt gttgcatctt tttgttccac gattttttct acgatcccaa
    21721 tatggtcacg gatgattcgt ccatatcctg ttgggttttc tgcttgtgcc gtcaagatcg
    21781 ttgcacttgc attttttcct tggtgatatt caaaaagatt gttcagcgtt tcagtagtca
    21841 ataatggtgt atcgccactg ataactaacg ttgttccttt ttttccttcc aagaaagaag
    21901 cagcttgcaa aacagcatgt cctgttccca attgttctgc ttgtaacgca tatttactgc
    21961 gttctcctag ttgtgcttta acttgttctg ctccgtgtcc aacgatcgta atcacttcat
    22021 ctggtttcgt ttcactgacc cgattaatga tatgttctac cataggttta ccagaaacgg
    22081 gatgtaaaac tttatataat tttgacttca tgcgggtacc tttccctgcc gccaaaataa
    22141 ttgcataacg tgcgtccaac tgcgtcactc ctaaatatag tttgctgatt tcagtttatc
    22201 ccagtatacc cattttttca acaaaacaag tgattttctt ttatcccagc gttttctgga
    22261 taaaaaaata gatagaccat aacgggccta tctatttggt aaccggtgct ctcctgccaa
    22321 ctcctcggtc acaagtcaca ctactcataa aacatgaaaa gctgtttttt gaaatcgttt
    22381 cttgtgactt agagttaaac ggtaggctta caacctcttc attaaacgtt tttcaatcag
    22441 ctgaccttcg gtattagctc aataaacggc aaaatatgag gagcaatttt tccgtttatt
    22501 gagctaatac tcaggacagg acgctgattt cacaacctct agataaacgg tgttcaaaag
    22561 ccagtttctc ggtattaagc caaatttatg aaaaaatgtg gaaagccatt ttcacaaaat
    22621 ttttcttact actcgaaact aagcggcttt ttcacaacct cttattctga aaaataattt
    22681 cctggcacaa ccgtaatatt tttcgtttga gtatctacat ctttgacata aagcagtgac
    22741 gtataatcat cgatggcgcg atgaccgttg aattttgatt cggcaaatac agtgatgcca
    22801 acaagttctg attcgaattc ttcaatcaag ctcttcattc cgttgactgt tccgccgcct
    22861 ttcatgaaat catccactac aaggacacgt gaaccacgtt tcaaacttct cttagacagt
    22921 tccatctttt caattctttc agaagatcca gacacatagt tgacacttac tgttgatcct
    22981 tctgttattt ttgagtcccg gcgtacaata acgaatggcg cattcagata ataggacaca
    23041 gcttgggcaa taggtacgcc ttttgtggct acagtcatta cagcatcgat tttttcacca
    23101 aggtatttag aagcaatgat tcttccaact tgacgcagca gttcaggttc tcctaacaaa
    23161 tcagagagat aaacatatcc tcctggaagc agacggtctt gttctgatag acgttctgct
    23221 agagattcaa tatatttttt cgcctcttca tatgaaattt cagggatgaa caatacacca
    23281 cctgcagcac ccgggattgt ttccaaggtt ccataccctc gttctttaaa tgttttcttg
    23341 atgattccca aatcttcact gatagaagat ttagcagatt cataacgttc ggcaaagctt
    23401 gttaaggaaa tcaattcatg cgggtgattc aataaatact gtgtcatgtc aactagacgt
    23461 tcactgcgac gtactttcac ctattccaac tccttttaag aaattttatc gttttacatt
    23521 atataagttt ttcttcaaaa attcgagcat tttatgaaaa aataagaaaa tagttcggta
    23581 tttattaaaa taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt
    23641 tataatttag ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat
    23701 caggaacaaa gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa
    23761 cgagctagtt agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac
    23821 gacattgaag cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga
    23881 tatcaaaagt gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt
    23941 gaacatcatc gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa
    24001 agtcagaaca tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa
    24061 gattcctaaa aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga
    24121 ttggatactt gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa
    24181 accaccagcc attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag
    24241 atactccaat atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag
    24301 gttcaaaaag aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat
    24361 cagcgattgt cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa
    24421 gatagcggca agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc
    24481 tcctttctca ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg
    24541 gtgatcatta gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat
    24601 tcatttctgg attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg
    24661 tcagtagcaa aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct
    24721 attcgccgat ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga
    24781 tgatcttttt cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact
    24841 aattcgatca ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa
    24901 ccgatactta atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa
    24961 ggtttcagta atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca
    25021 gttaaaatga caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca
    25081 tcatcatagt aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc
    25141 taaaacttaa ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata
    25201 attttcgcct gctttgattt gttcattagt caagctttct aaaggattca acacttctag
    25261 cttcactcct gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa
    25321 ataaatcact ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg
    25381 tgtgggttcc tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt
    25441 cgctaaataa gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag
    25501 ctcatcggag tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt
    25561 cgtaaaagtt tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac
    25621 ttcttttata gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc
    25681 atgcccttct tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc
    25741 tttagctgct tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac
    25801 aaaaaattcc atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct
    25861 ttcttttgca cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac
    25921 gtttccttca tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac
    25981 gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag
    26041 taaagcacct atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc
    26101 aatgaacaaa tcggaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa
    26161 cacagtcaat agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt
    26221 aaaatggatg agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata
    26281 ccattttagg attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg
    26341 ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc
    26401 ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat
    26461 ctgctccgta cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg
    26521 ttatatcttc taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga
    26581 tggcttcagc aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa
    26641 acactgtacg tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca
    26701 tagaaggtaa tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga
    26761 cacagtaagg aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac
    26821 agcccagatt ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc
    26881 cgcctaatcc agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga
    26941 tattgtattt attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg
    27001 gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata
    27061 gatgatctgc caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc
    27121 gtctatataa tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca
    27181 tcctctcacc cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag
    27241 tatagcttat gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt
    27301 ttctcttcat aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg
    27361 ccaaactcgg aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa
    27421 atccgaaatt acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt
    27481 ttaatttctt ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga
    27541 tatgcatgac tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag
    27601 ctaagcctag attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta
    27661 acagatagtc agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg
    27721 cctgcttctt cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga
    27781 caataccgac accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt
    27841 tttctcgtgc accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt
    27901 ccggcagttg atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa
    27961 gttgctgact cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta
    28021 ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat
    28081 aaaacgcaag ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc
    28141 gcatcatttt aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct
    28201 gataaccaaa aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg
    28261 acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac
    28321 ggtctttttc gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg
    28381 ccactaaagc tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg
    28441 aaggaaacaa aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat
    28501 agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag
    28561 ctccccaaga ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta
    28621 acagaatcaa gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta
    28681 aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa
    28741 gtgctctagc aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa
    28801 aattgaatga gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga
    28861 ttgttgatag aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat
    28921 gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc
    28981 ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc
    29041 gataatataa tagccaaact actgctacat cttgccgaac agacaattga ctcattccta
    29101 agatcatcaa aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca
    29161 atgtagcaat catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact
    29221 tgccgatgat caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca
    29281 gaccaagcaa gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa
    29341 aaaagaaaat gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa
    29401 aaatactttc tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca
    29461 aaccagtcag cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg
    29521 tctgctttcc ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga
    29581 gattgttgat cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg
    29641 gttttccttg tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga
    29701 cacccagcag tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc
    29761 tttgccaaat atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa
    29821 agactaacac ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt
    29881 gcagcatcaa gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg
    29941 tcatacccca atgttatctt atttatttta atttttccac tttagacctg ttctttgctt
    30001 gcgatgatag aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga
    30061 tttatcggca cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca
    30121 gtctcgaaag aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct
    30181 gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc
    30241 gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat
    30301 agccagaaag tcttattcat ttctattgct tcaggaaaat cattaataga tttagaaaca
    30361 gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta
    30421 ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa
    30481 gctacgatga tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt
    30541 acattcattg gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg
    30601 ggacggactg gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa
    30661 gcaattatgg gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta
    30721 gaccaagttt cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca
    30781 ggattcatcc cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg
    30841 ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg
    30901 gcttttgccg cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt
    30961 ttcccgaata acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt
    31021 acgaatatca cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat
    31081 tacccgaact tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt
    31141 cgaaatattt tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc
    31201 accaggtctt gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt
    31261 agcttccaca tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta
    31321 cttggcagct aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc
    31381 tttcccaata tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc
    31441 tgctgctaag ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc
    31501 ataactagtt ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg
    31561 agcaccgact tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt
    31621 acgagatttc tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag
    31681 tttttccaag gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac
    31741 tgcttccggt gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat
    31801 attacgacct tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac
    31861 tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa
    31921 gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag
    31981 agttaactca tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc
    32041 ttttgataaa ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac
    32101 gtctttttct cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc
    32161 aagtgagttc tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg
    32221 aatcaaacga ttctcttgag attttaattc taacttactt tctctcagct cactttcaac
    32281 ttcagaacgg tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt
    32341 ttctgcttct ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat
    32401 ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc
    32461 gatgatagcg aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt
    32521 tttatcatct atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc
    32581 atatatgtgt acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa
    32641 ctttaaataa acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaataa
    32701 atgacactag tttcatatag gtaaaccttt aaattgagaa acttttctat taaattattc
    32761 tttgtagatg aacagatatt tttattttat gtacccactc gtataattaa ataactcacg
    32821 atttctttat tcaataaaaa aacggcaggt caaggagagt acctactctt cagaccagcc
    32881 gtttttgttt atcataattt ttattcttct actaaaggca attcttcttg tcctttttca
    32941 tcttctggga cagcgacttc ttcaccaata ccataagctg ctctaactag agcagaaact
    33001 tctgccatca tttcaggatg attagccata tagattttag cgttttcccg tccttgacca
    33061 atccgatctt ctttataaga ataccaagca ccgcttttat caacaatgtc tttttctact
    33121 gccatgtcta gaagttctcc ttcttgtgag atccctaaac catacatgac atctacttca
    33181 gcgattttga aaggaggtgc tactttgttt tttacgactt tgatctttgt acggttacca
    33241 acaatatctg taccttgttt caattgttct gcacgacgta cttccaaacg gatcgttgcg
    33301 tagaatttca atgcgcgtcc tccaggagtg atttctggat tcccaaacat cacgcctact
    33361 ttttcacgga tttggttgat gaaaatagcg attgtttttg ttttgttgat cgaaccagag
    33421 agtttacgca atgcttgaga catcaaacgt gcttgtaacc cgacatgtga gtcacccatt
    33481 tcgccgtcga tttctgctcg tggaactaaa gcagcaactg aatcgactac tacgatatct
    33541 acagccccac ttgatactaa agcatcagcg atctctagac cttgttctcc tgtgtcaggc
    33601 tgtgaaagaa gtagttcatc gatattcaca cctaattttt gtgcatattg cggatctaac
    33661 gcatgctcag catcaatgaa agcggccgtt ccgccatttt tttgtacttc tgcaatagcg
    33721 tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat atacttcgat gatacgtcca
    33781 cgggggtaac cgccgacacc taaagcgaca tctaacgcaa gagagccact tggaattgta
    33841 gagatttgtt ggtcgatttt ttcgcccaat ttcataatag agcctttacc atagctcttt
    33901 tcaatttttt tcagtgcagc atctaaggct gctttacgat catctgccaa tacgataatc
    33961 ctccttatat ttacaaatca gagcaacatt tcattatcct tatcatatct tttttagtga
    34021 aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc attttcttaa aagctgtttt
    34081 ctcaaaagat caaatccttg cataaccgca ttatatctga tagcagcacg atctctggca
    34141 aagtgatatt tttccgaaac cacgccgtct ttagatgaaa tagcgatcca tactgttcct
    34201 gcagattgac cttctaacgt gtccggtcca gccactccgg tgaaagctac actgtaatct
    34261 gttcttgcta tctcacgtgc ttgaatggcc atctgctcca cacattcttt gctgactgtt
    34321 ccatattctg ctaatagctc cggatcaatc tccagaaaac cagcttttgt ttggagcgag
    34381 taggtaacga atccgcctgg aaatacttca gatacaccag caatgtcgcc taatgcggct
    34441 tggaaggcgc cggctgtcaa actttccgct gctgttacag tctgcttgtt ttctttcagt
    34501 agttccacta cgacttttgc tagcgaatta tcatcgccgt atccataaaa atattcccct
    34561 actcgttctt ggattttttc ttctaatgct aaaagagctt gattccctgc ctgaacatca
    34621 tttgttttca ctgtcagtcg taatgtcact tcatttggtt tggcatatgg tgcgatcgtt
    34681 ggattgatct gtgtctcaat cagatctttt agctcagtga ccagtcgcga ttcaccaatc
    34741 ccataaaagc gcaaaacttt agaaattagt ttttcttcag aaggaaaacg ttcctctagt
    34801 aaaggacgta cttgttccac aaacattggt ttcaactcat taggcggacc gggaagaagg
    34861 atatacgcat gtgtatctgt ttgataaaaa gtccctaaag caagccctgt acgatttggt
    34921 agaggaaccc cgccttcgat aatctgggac tgttgcagat tgtttttggt catttttcga
    34981 tgggttgttt caaaatatgc caataatttt ttgtatcctt ccgtattttg tatgagagac
    35041 ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg
    35101 ccacataaga gtatgagttc acttcttgtt tcagctagag caatcaactc ttctaaacgc
    35161 tgctgattat ctcctacaac tgtttgataa taaacttcga tacctagatc agctagctgt
    35221 tctgacaaga aagtggcatt cgtattcacg acttgtccta gtaaaagttc tgttccgaca
    35281 gctattattt ctgctttcat tacttcccgc tcccttatat agtatacgct gtatttttat
    35341 atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt
    35401 tccaaataaa cagtgttctc ctgccaaaac ctcggtcata agtcacaata tacataaaac
    35461 atgaaaagct gttttttgaa attatccctt gtgactcaga gttagacggc aggctcacaa
    35521 cctctccact aaacgatact tacattgatc ctttgaacac accgctgttt ttgatgaagt
    35581 aatccacacc tgaatagata gtaaagatca gacaagcata aagcatgatt tgatctactg
    35641 gaataccgat caggctgaat gggatattat tgataaacag caagataata gcaaccattt
    35701 gtgtagccgt cttgactttt cccggccaag ctgcagccat gacttctcca tgttcgacaa
    35761 gtagtaaacg caagcctgtg acggctaatt cacgacagac aatgatcgct actacccaac
    35821 caggggcctt tccttggtca accaacatga taaaagcagt cataactagc attttatctg
    35881 ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc
    35941 catctaacca gtctgtgata ctagctaaag caaaaataac tgcacctact agctgtgtga
    36001 cagccagtgt ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa cctacaacca
    36061 aaatgaaaat cgggatcata caaattctaa gtacagttaa tttatttggt aaattcacac
    36121 tatcgactcc tttcttccct attctacgtg actatcagcg aagtttccac cgatattatt
    36181 tacttttcca aatgaattta ttcaaaggag tatttttatt gaaattgatc tttttgaatt
    36241 aacgaagaag gaccgcgaca cagatttgtc acgatccttc ttttttatac ctacaagtaa
    36301 acagatgcgt aaactaattt atacgatcat tctgcgtatt gaagagtaag gttcacattt
    36361 tttggactct gattgttttc ccctggatta actggtacgg attctccatt cgctttgatc
    36421 gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg
    36481 gtactctgtg tttcatttgc tgccagtgtc ccttgataaa cataggcatt gtctactagc
    36541 acacctaacc atacacgatt gacagcagta aattcaaggg ttacaggctt ggtagcgtct
    36601 gtgatattta ctgcgatggc actgccagaa tcttctcctg tagctattgc cattttcttt
    36661 tcttcacttg attttgttgt tgattctact gtagaagact ctttggtctg cgtgctttgc
    36721 tctgaggaag aggactcctc gactgatcct tctactttaa gcgaagaagc tgtgtctccg
    36781 attattggat cagcttgacg atcttgccaa gtcatatagc ccacgacaac gacaatcgcc
    36841 aaagcaacca atcctaataa aatgacagga agacttgtcc aaaagcgact tggatgcgat
    36901 tcttcctgat gcattgcttt tctagaagta cttactgttt caggctgcgg acgttcaggg
    36961 accgatcctg tcaatttgct tttcccatca aaaacatcga ctaaatggtc tccatcttct
    37021 cctacagctt cagcgtattg ccgaataaat gcccgcacgt aaaatttacc aggcaattgg
    37081 tcaaaatcat cattgtcgat tgcttccaaa taacgttttt gaatttttgt aatctgctgt
    37141 aattcatcta aagatatttt tttattcagg cgtgcttgcc gtaatttttc tccgatggtt
    37201 tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc tccgaagcat ctgctgttgc
    37261 aacgaaagac gttatgtttg ggtgctgtga caaaagtcat gtcacagctc cttttttccg
    37321 aataaacatt gttctcctgc caactcctcg gtcacaagtc acaatatcca ataaatataa
    37381 gaagctgttt tttgaacttg ttccttgtga ctcggagtta aacggcaggc tcacgacctc
    37441 tttcttaaac ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat
    37501 aaggagatat ttttccgtat gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    37561 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
    37621 acattaagaa gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca
    37681 caacctcttg gtacacggtg ttccagaagt agcttcttcg gcaataagcc gaaatatcac
    37741 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
    37801 tcacaacctc tttgattact tcgtttgatt ttattttaat ataagttgat taaaagagag
    37861 catgatttgt tttattttct cactaatttt tccaacacaa ctaagaatac cataaaagct
    37921 gattttggag gaggttccta ctacaagtct aaaacgaaat ttaagacttt tcctcataaa
    37981 atactggttt attttggtct catgtaaaac tcgctgaagg cctcttcact tataaatgct
    38041 tccccagctg ccagtacgtc tttcatttgg atcgaatcga tgatttcagg aagatcaaat
    38101 aaagttcgat cgccaaataa actttgcgta aattgattgg caatatattc gatcgaattt
    38161 aatgattgga aatattgtcc aagcattttc ttcttcagca aatcgagatt tgtttctgac
    38221 acttccggat catcagcgaa tccaagaatg atttctttga ctttttctgc tgctttctct
    38281 ggttcgtccg tatctccgct gaagtccgca aagtgaaatt cacgatccaa gctgaattca
    38341 aaaccaaagc tgtcatcgat aattccttga ttgtacatcg ctagatagtt tcgagaagta
    38401 ttgcctaaaa tcatttggaa caacagattg attgctgttt tatagcgtaa cagttctgtt
    38461 ccttcttgcg gcaatgtgtc taatccttta ataccaagta caaatttatc tcgtgtaatg
    38521 gctgcttcca atgcactttg tttgatgatt tcttttgtgt tttcagggaa ataacgtaca
    38581 atttcttgct ttggagggaa attttttgct tcttggtttt cccgaataag tttcatcaat
    38641 ttctcaggct ccatcttacc aactacaaat aaaaccatat tgcttggctg ataaaacgtc
    38701 cgatagcacg tatacaagtc ttgagctgtg attttgtcga tactttcaac cgtgcccgca
    38761 atatcgatat gcaatgggtg tgtaggatac aagttattta gtatgccaaa aaacatccgc
    38821 cagttagggt cgtcttcata catttggatt tcctgaccga tgattccttt ttccttgttt
    38881 acagtttctt ctgtgaagta aggtgcttgt acaaaatcga tcagagtcgt cagattcttt
    38941 tctacctgat ccgtcgtaga aaacagatag ctggttttcg taaaacttgt gaaagcattg
    39001 gcggaagctc cttgtttccc aaataattga aagacatcac catcttcttt ttcaaacaac
    39061 ttatgttcta aaaaatgggc aattccatct ggaactttca ccttttcttt ttcaccatag
    39121 ggaataaatt cgttatcgat cgaaccataa ttcgtactga acaaaccata tgtcttgtga
    39181 tagtcatttt taggcaacaa atacacggtc aaaccgtttg gcagtacttc atgataaagt
    39241 gtttcattga tttgctcata ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa
    39301 gattgcctgc aattcgattt gctcagctac ttgttgtatc tctttgatcg ttacgctctc
    39361 catacgtttc aaccattctt catcagataa ttttgtttcc ggcagccagc tgtccaaaaa
    39421 agaagcttcg attgccgctt gcggagagtc tagagaaagc agatattggt ttctcagcat
    39481 tgcttttgtc tgagacagtt ccaactctgt tatttcacca tttcgcaaac tttctaattg
    39541 ctcgtgaatc agatgaagta cttgatttcg gttcttttca tcgataccgg tttggacact
    39601 catgaatcct cggaacgtat ccacgctact agaggcataa taagctagac tttctttctc
    39661 tctgacattc atgaaaagtt tagagtgagg aaaaccgcca aataaaccgt taaagaccat
    39721 tagcgcaaaa cgttctggtt catcgtaata cacatttgtc tgataagcta ggttcagctt
    39781 tgcttgaacg attggctcac gtacttgtcg ttccttcacg atattcactt gaggttgggt
    39841 ataaaacaga tctggataaa ctgtttcccg cgactcaaat ggtaattgcc caactgcttc
    39901 agctgctttg tccggatcaa catcgccgac aacaaaaatg tcgatttgat catttgccat
    39961 catttcttga tagtaggacg ctagcgaact agcagtgatc ttgtccaaat cttcgactac
    40021 gcccaaccct gggatctttt gttccgggct gttttcaaaa taaagttctt gcaaggcata
    40081 ggaagcaaaa gtttgtttat cttccttcaa actttccaaa tatagacgca gattgttctt
    40141 ttccaagtca aacgtcaact gatcaaactg atgattttta atatttggat aaaataaaac
    40201 ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga tcattgacat attcaccatt
    40261 cacaaaactg atccctgcgt ttagccaatg taaatttcct ttttttctaa ctgataatcc
    40321 aaagcttgcg ccataaagct ctgcaagttg cgcacttaac tttgtctgat ccgaataatt
    40381 caaactattt gtttccaaca tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg
    40441 cagacgcgca gtaaaacgga taaaaatccg gatcgttttg tattgttttg ttggtaagac
    40501 atgaagattc acgccttctg ccaattgata caccatattt atatgctcct tttcacagtt
    40561 gattgcccgc ttactctttt aatgggtcaa cctgcctttt ttacttattc ctctgcttga
    40621 aatttataag ttcaggcaat tcgccgtttt ttctaaatga tgttacactt tatagtatag
    40681 catagatgtt ttttatcaaa agaaacagac ggctaaaagc gtatattgtt tttttatttt
    40741 ttatttttcg atatactaaa gacaaataaa agaagggagg gtcattcatg atcaaagaat
    40801 tcaaagagtt catcatgcgt ggagatgtct tggatcttgc tgtcggcgtt gttattggta
    40861 gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt gatcacacca ctaataggtt
    40921 ggtttgtcgc aatgatcaca ggtacttctg atttagaagg agcactttca attcttgact
    40981 ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt tattagtgct atcattacat
    41041 ttctgatcac tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt
    41101 tacgctccaa agaagagcaa gtggaagtgg aagaaactcc tactgcagaa gattatctta
    41161 gtgacatacg cgatcttctt gccaaacaag tggcagaaga caatacacat ccagtagagc
    41221 aaacaaatct ttccgatact tctaaataat aaagaatcta aaaaaaagaa agccgatttt
    41281 cagtgtcggc tttctttttt tagattctta acttgctact tgttcctttt tcatgtcgat
    41341 atgtggaata tcatcttcca aatacacatc tgagattgcc ttgaatccaa acgactcata
    41401 aaaagcttgt aggtattctt gtgcgccgat cacgattggt ttttctggaa aatttttctg
    41461 gatcatgtta atcgtttctt gaacaatctt tcttcctaaa ccatctttac ggttgtcctg
    41521 atgaacaagg acacgtccaa aatggatact ctccgcttct tcatatactc gcgtgtaggc
    41581 aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc
    41641 aacttcttga taaggacact gttgttccac aacaaaaact gcgattcgtt ctttgacgat
    41701 cgcgtaaaac tctgctgttg acaattcttc aaatttcttt acctgatatt ccactaagca
    41761 aacgccccct gcttttttat tcttagcata ccgattcgca cacaaaaaag caaacgaacc
    41821 ttacgaatct caggctcgtt tgctttttaa actgcttcag ttaaaaattc gatctcaact
    41881 gtccgagtaa gtacatctga atagctgtaa gatactcgtt caaaagaatt ttcttcagga
    41941 tccaaatcaa caacgaatac tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt
    42001 tgacgctttc ttcctgtctg agcaaccaac atgattgggc tgccgatacg accttctaaa
    42061 tctttcttga ttgaagctaa agttgttggc atacagttca cctctactat caactatacc
    42121 acatattttt aaaattttca agtttaccat aaaaaaaaaa gaaatgcaat aaaaataaat
    42181 aagaagctca ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt
    42241 attttgagtt ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat
    42301 tcagccatgt ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt
    42361 tttgaaaact ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaattgct
    42421 aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg
    42481 tcgattgaga cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga
    42541 gaatagattt gattgcttat aacgctttta taataacttc caaagctaac acctttgctt
    42601 ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca
    42661 gaaacataat ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt
    42721 tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca
    42781 gacataaaat aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat
    42841 cgaactgatt agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga
    42901 caattttttt aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat
    42961 atctttattt tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata
    43021 agcagaagtt cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt
    43081 cgccactgtc acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact
    43141 gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg
    43201 ttggatgccc ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt
    43261 cgcataattt gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt
    43321 tttaagctgg acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag
    43381 ctgttttttc atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc
    43441 agcagcaaca ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc
    43501 atccatttct tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc
    43561 aattgatcct ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac
    43621 ccaaaatcct gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac
    43681 agggacatgt tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc
    43741 tcggtgtttt ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga
    43801 cccaaaattg tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt
    43861 ctcttttgtt cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag
    43921 aacgactcct tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca
    43981 tttgactggc acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg
    44041 tgaatcttct tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc
    44101 atggtgccca aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg
    44161 atttcgtcca gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg
    44221 tttcgattga tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac
    44281 accagcgga
//
LOCUS       DACEDM010000011        43590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEDM010000011 DACEDM010000000
VERSION     DACEDM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.011"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            160..840
                     /locus_tag="IWO50_000603"
     CDS             160..840
                     /locus_tag="IWO50_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0357746.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="IWO50_000604"
     CDS             930..1964
                     /locus_tag="IWO50_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0357747.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="IWO50_000605"
     CDS             1978..2313
                     /locus_tag="IWO50_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357748.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="IWO50_000606"
     CDS             2297..4777
                     /locus_tag="IWO50_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357749.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="IWO50_000607"
     CDS             4833..6392
                     /locus_tag="IWO50_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0357750.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="IWO50_000608"
     CDS             6389..7753
                     /locus_tag="IWO50_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0357751.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="IWO50_000609"
     CDS             7924..8994
                     /locus_tag="IWO50_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0357752.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="IWO50_000610"
     CDS             9009..9677
                     /locus_tag="IWO50_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357753.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="IWO50_000611"
     CDS             9697..10275
                     /locus_tag="IWO50_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0357754.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="IWO50_000612"
     CDS             10676..11587
                     /locus_tag="IWO50_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0357755.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="IWO50_000613"
     CDS             complement(11965..12882)
                     /locus_tag="IWO50_000613"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0357756.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="IWO50_000614"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="IWO50_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0357757.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="IWO50_000615"
     CDS             13579..14127
                     /locus_tag="IWO50_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0357758.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="IWO50_000616"
     CDS             14242..16602
                     /locus_tag="IWO50_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0357759.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="IWO50_000617"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="IWO50_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0357760.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="IWO50_000618"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="IWO50_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0357761.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="IWO50_000619"
     CDS             19108..20007
                     /locus_tag="IWO50_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357762.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="IWO50_000620"
     CDS             20000..21262
                     /locus_tag="IWO50_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0357763.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="IWO50_000621"
     CDS             21423..23321
                     /locus_tag="IWO50_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0357764.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="IWO50_000622"
     CDS             complement(23651..24370)
                     /locus_tag="IWO50_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0357765.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="IWO50_000623"
     CDS             24707..25039
                     /locus_tag="IWO50_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357766.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="IWO50_000624"
     CDS             25026..27029
                     /locus_tag="IWO50_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0357767.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="IWO50_000625"
     CDS             27048..27518
                     /locus_tag="IWO50_000625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0357768.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="IWO50_000626"
     CDS             27539..28123
                     /locus_tag="IWO50_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0357769.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="IWO50_000627"
     CDS             28138..29124
                     /locus_tag="IWO50_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0357770.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="IWO50_000628"
     CDS             29114..29425
                     /locus_tag="IWO50_000628"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0357771.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="IWO50_000629"
     CDS             29447..31228
                     /locus_tag="IWO50_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0357772.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="IWO50_000630"
     CDS             31221..32597
                     /locus_tag="IWO50_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0357773.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="IWO50_000631"
     CDS             32604..33239
                     /locus_tag="IWO50_000631"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0357774.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="IWO50_000632"
     CDS             33269..34624
                     /locus_tag="IWO50_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0357775.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="IWO50_000633"
     CDS             complement(34816..36210)
                     /locus_tag="IWO50_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0357776.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="IWO50_000634"
     CDS             36302..37273
                     /locus_tag="IWO50_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0357777.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="IWO50_000635"
     CDS             37270..38052
                     /locus_tag="IWO50_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0357778.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="IWO50_000636"
     CDS             complement(38221..38592)
                     /locus_tag="IWO50_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0357779.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="IWO50_000637"
     CDS             38726..39169
                     /locus_tag="IWO50_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0357780.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IWO50_000638"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IWO50_000638"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0357781.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="IWO50_000639"
     CDS             complement(40040..41407)
                     /locus_tag="IWO50_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0357782.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="IWO50_000640"
     CDS             complement(41391..42221)
                     /locus_tag="IWO50_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0357783.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="IWO50_000641"
     CDS             42384..42821
                     /locus_tag="IWO50_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0357784.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /locus_tag="IWO50_000642"
     CDS             42897..43517
                     /locus_tag="IWO50_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0357785.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14279 a         7487 c         9251 g        12573 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggatc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt
//
LOCUS       DACEDM010000012        43048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEDM010000012 DACEDM010000000
VERSION     DACEDM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.012"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(658..1014)
                     /locus_tag="IWO50_000643"
     CDS             complement(658..1014)
                     /locus_tag="IWO50_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0357786.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(1028..1438)
                     /locus_tag="IWO50_000644"
     CDS             complement(1028..1438)
                     /locus_tag="IWO50_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0357787.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1785..3971)
                     /locus_tag="IWO50_000645"
     CDS             complement(1785..3971)
                     /locus_tag="IWO50_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0357788.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4189..4872
                     /locus_tag="IWO50_000646"
     CDS             4189..4872
                     /locus_tag="IWO50_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0357789.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4953..5672)
                     /locus_tag="IWO50_000647"
     CDS             complement(4953..5672)
                     /locus_tag="IWO50_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0357790.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5984..6190)
                     /locus_tag="IWO50_000648"
     CDS             complement(5984..6190)
                     /locus_tag="IWO50_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357791.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6435..7037
                     /locus_tag="IWO50_000649"
     CDS             6435..7037
                     /locus_tag="IWO50_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0357792.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7178..8458
                     /locus_tag="IWO50_000650"
     CDS             7178..8458
                     /locus_tag="IWO50_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0357793.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8471..9199
                     /locus_tag="IWO50_000651"
     CDS             8471..9199
                     /locus_tag="IWO50_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0357794.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9268..10791)
                     /locus_tag="IWO50_000652"
     CDS             complement(9268..10791)
                     /locus_tag="IWO50_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0357795.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            11043..12224
                     /locus_tag="IWO50_000653"
     CDS             11043..12224
                     /locus_tag="IWO50_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0357796.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12284..13912)
                     /gene="treC"
                     /locus_tag="IWO50_000654"
     CDS             complement(12284..13912)
                     /gene="treC"
                     /locus_tag="IWO50_000654"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0357797.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14363..17074)
                     /locus_tag="IWO50_000655"
     CDS             complement(14363..17074)
                     /locus_tag="IWO50_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0357798.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17084..17785)
                     /locus_tag="IWO50_000656"
     CDS             complement(17084..17785)
                     /locus_tag="IWO50_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357799.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17971..18570
                     /locus_tag="IWO50_000657"
     CDS             17971..18570
                     /locus_tag="IWO50_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0357800.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18651..19664
                     /locus_tag="IWO50_000658"
     CDS             18651..19664
                     /locus_tag="IWO50_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0357801.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19772..20689
                     /gene="rbsK"
                     /locus_tag="IWO50_000659"
     CDS             19772..20689
                     /gene="rbsK"
                     /locus_tag="IWO50_000659"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0357802.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20717..21112
                     /gene="rbsD"
                     /locus_tag="IWO50_000660"
     CDS             20717..21112
                     /gene="rbsD"
                     /locus_tag="IWO50_000660"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0357803.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21112..22485
                     /locus_tag="IWO50_000661"
     CDS             21112..22485
                     /locus_tag="IWO50_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0357804.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22842..24986
                     /locus_tag="IWO50_000662"
     CDS             22842..24986
                     /locus_tag="IWO50_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0357805.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(25038..26078)
                     /locus_tag="IWO50_000663"
     CDS             complement(25038..26078)
                     /locus_tag="IWO50_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0357806.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26355..28157)
                     /locus_tag="IWO50_000664"
     CDS             complement(26355..28157)
                     /locus_tag="IWO50_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0357807.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28171..28923)
                     /locus_tag="IWO50_000665"
     CDS             complement(28171..28923)
                     /locus_tag="IWO50_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357808.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28932..29294)
                     /locus_tag="IWO50_000666"
     CDS             complement(28932..29294)
                     /locus_tag="IWO50_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0357809.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29470..30342)
                     /locus_tag="IWO50_000667"
     CDS             complement(29470..30342)
                     /locus_tag="IWO50_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0357810.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30393..30881)
                     /locus_tag="IWO50_000668"
     CDS             complement(30393..30881)
                     /locus_tag="IWO50_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0357811.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(31019..31849)
                     /locus_tag="IWO50_000669"
     CDS             complement(31019..31849)
                     /locus_tag="IWO50_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0357812.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(31865..32473)
                     /locus_tag="IWO50_000670"
     CDS             complement(31865..32473)
                     /locus_tag="IWO50_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0357813.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32466..32783)
                     /locus_tag="IWO50_000671"
     CDS             complement(32466..32783)
                     /locus_tag="IWO50_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0357814.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32939..33667)
                     /locus_tag="IWO50_000672"
     CDS             complement(32939..33667)
                     /locus_tag="IWO50_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0357815.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33826..34707
                     /locus_tag="IWO50_000673"
     CDS             33826..34707
                     /locus_tag="IWO50_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0357816.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34944..35987)
                     /locus_tag="IWO50_000674"
     CDS             complement(34944..35987)
                     /locus_tag="IWO50_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0357817.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35990..36658)
                     /locus_tag="IWO50_000675"
     CDS             complement(35990..36658)
                     /locus_tag="IWO50_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0357818.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36800..39136
                     /locus_tag="IWO50_000676"
     CDS             36800..39136
                     /locus_tag="IWO50_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0357819.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39251..39562)
                     /locus_tag="IWO50_000677"
     CDS             complement(39251..39562)
                     /locus_tag="IWO50_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357820.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39743..41218
                     /locus_tag="IWO50_000678"
     CDS             39743..41218
                     /locus_tag="IWO50_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0357821.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41265..41849
                     /locus_tag="IWO50_000679"
     CDS             41265..41849
                     /locus_tag="IWO50_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0357822.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41851..42270
                     /locus_tag="IWO50_000680"
     CDS             41851..42270
                     /locus_tag="IWO50_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0357823.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42551..42835)
                     /locus_tag="IWO50_000681"
     CDS             complement(42551..42835)
                     /locus_tag="IWO50_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0357824.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
BASE COUNT        12829 a         8603 c         7574 g        14042 t
ORIGIN      
        1 cgattgttaa gctcaatttg tttgctagca tattgcttgc ttgttaaaaa atgtttgttg
       61 tcttgaattg aatcaagacg ccctacacat ttggtttgtc ttactttgtt cagttttcaa
      121 aggtctactt catcaggagt gttccccgat tcgtttttgg ctgccgtagc aacttttata
      181 tcatatcaga gagacaagta attgtcaact cttttttaaa agtttttttg atttcgtttt
      241 tcgaaatctt acagcaactc atttatcata acttatcatt tgttatttgt caagaacttt
      301 ttttgaagtt tttttcgata gaatcaagct tcacaagtgg ttctttttta acaactcagt
      361 tatcttatca tgtcatttct aacatgtcaa caactatttt atatgttttt taaaatacta
      421 ttttattgct ttgctactta aaacaacaga aatgaatata tcatgtaaat ttacacatgt
      481 caatatattt ttaactctat atccccacct agatttgaat tttgaccaca taaattgagc
      541 atgtctctat agtagaaaaa taaagtcaaa aaaagaacac ataacacgct tgatgctaag
      601 tgttcgactg ttacaactgt atgaaatgct cagtgacttg attaaaacag ttaaagtcta
      661 tttatttaag ataagctgtt ccatcaactg ctcattgtat ccaataaata gaacttcatc
      721 gataataatc aaaggacgtc tgaggagatt ggtatgttgt gtgcataaaa aaatcagttg
      781 tttttttcct acacaggaaa ggtcaatcgc aatgttgtga aaagcctcag accaagtcgc
      841 aaatagctgt tctgcttgca cttcttcaaa actgcataat tcttctgctt cttctgtact
      901 tagatcgcgt tcttctaagt caacaaattg ataaggcaat tgatggtgtt ctaaccattg
      961 tttcgccttc agacttgtta cacagtgcgc tgatccatag actttcactt ccacgaaggc
     1021 tccctcccta tgaatttaag atgttattgt tttgtaaata gatcagattt ctggcaacac
     1081 atcgaatttc acgagggaca aatgaccgga tctcatcttc attaaatccg attagtattc
     1141 ttttttcatc aatcaaaata ggtcgtctca ataatgaagg atattctttg aataagacga
     1201 tcatttgctt taaagttaat tcgttaaaat ctatgcccag cttttcgtaa attttcgacc
     1261 gagtggaaat aatatcttga actcctcctt cagtcgctgc caagatcttt ttgatctcac
     1321 tttctgttaa cggttcggca aaaatattct tctcttcaaa ttcaagttga ttcgcttgca
     1381 accatgctct tgcttttcta caggaagtac agctaggcga agtatatact tttatcattt
     1441 tctcactcct ttttaattat aatccaattc tacagtaatt tcttttaaaa taccaattat
     1501 tagctaataa aatataaaaa aaaatcaaaa taactgatta tttacgtttg aataatgaga
     1561 atttttctat atattctcta ttttgataat agtattctca ttttcaaaat aaataaggga
     1621 ggtgtgacaa aattcttgtc acacctccct tttccgaata aactgtgttc cagaaatagc
     1681 ttcttcgaaa ataagctgaa atttcacaaa aatttgagaa gcaattttcg tacattcctt
     1741 cttctttctt gaaactgacc acttctgtca caccctctct tctatcactt tccttttttc
     1801 acacgactcc cggtttcttt gataaatctc gatactttca attttcttga atttttcatt
     1861 tgaggtacac atagatacag acgatttctt gctctagtca tcgcaacata aaatagtctt
     1921 cgttcttctt ctatcgtcgt cactggttct gcttttattt cattaaagtc gattactttc
     1981 ttttctgtcg ctccgttata aggcaaactt ccttcgttac aaccaatcaa aaagacattt
     2041 cggtaactaa gacctttact tttgtgaatg ctctggatac atagtgcatc aagttttcct
     2101 tgtctgtaag caatcaagcg ggtttttaat tctttagctg tcgcttgggt ttcttgcgat
     2161 aaggcttgga gtcttctgaa agtagcatgc tgctttactt cttctcgtat ccattcttga
     2221 tcactctggt cgatttcctt agctatacgt ggcagatgaa gatagctttg aacaagcgaa
     2281 tagatttctt gatgctggtc tgttttacgc aacgcttcct caaactctcg gatttctgca
     2341 gatttcgatt ttaaattggg gcttatcgac aaaatataat ctgcaacggc tatattttga
     2401 tatttaggag aaaagtattg atcgcattca ctttttttca ggtagcagtc tgccatcagc
     2461 tgccggagca attgctggta ggcctgattt ttcttttgca gattcagtgc tgtttgccaa
     2521 tgaacaattt tttgatagaa atgttggaaa gacttgatac caaagatacc ttcatatgga
     2581 ctatctgctt ccagtacaaa aggaatattc gttttcagca gagattggac aagctgctgc
     2641 gtttgtgaag ctgatcggta taatatgatt gattccttat atttcccatt tttttgaata
     2701 aatgtctgaa tttgtttgat tagccactga ctttctgctt tagtatcaga aaacaaatag
     2761 gcttccataa cacctgcttc tttttgtgct ggaatcatcg cctttggtat ccgatcattt
     2821 ttattatagc tgatcaatct atttgctcct tggacaatag ctgcccgaga acgataatta
     2881 gttaataaga aaagagtttt ggtaggatat tctttttcaa agttcttgat gtatgctggc
     2941 tctgatcctc tgaatccata gatactctga tcatcatctc caacaacaat caacttgatc
     3001 ctgttttcct ttgtcatccg tttcacgatt tcccattgaa gtggattagt atcttgaaat
     3061 tcatcaatga atagataatc atatttttgg ccgatataat gctggatctc ctcattctcc
     3121 aaagcgcttt tcagattttg gagtacatca ttgaactcta gcaattgctg gcttcttttt
     3181 tgttcttgga gttccgtgag caatccatac agtcgttctg atatcggctg tcgtgcattc
     3241 ttatcaatgg aatcatattt ttctttaact aactgaacca tttcttctac tgaaaggttc
     3301 atattctgcc aatagtctaa ccgagaagca atcgtttttt ctccatattg atcaaatata
     3361 taaagattat atttacgtat cagctgttgc aaaacagcat tcactttttg tgcagtatcc
     3421 tttgaaaagt ctccttgtat tccttgcatt gctacttcag gaaattttcg acaaatttca
     3481 ttgtatatcc taaaaaagat actatggaat gttccgatat ggacagcggt ttttctctgg
     3541 ctgaaattat ctactcgttt ttttagttca tttgcagcgt tccttgtaaa agtgatcatt
     3601 agtatgcgtt cttcgtctac ttgtttttgc gtcatcagat aaacagcttt tgcacagatt
     3661 gtttgcgttt tgccgctccc agctcctgct atgattcgaa gatgtttctt catatcatat
     3721 gtgatagctg ctttttgttc ttgattgaag cgattatagg aacgtttctc catcatttta
     3781 aagaaagagc tgtttccttc actttctttt acttgtactt cttcctcaat aagtccgggt
     3841 gcttcgccat aagccgcttc ataggtagca atgatttctt cttgtagccg aaagacttgt
     3901 tgaagctttt tatacttgtt ctcccagcgt ttcaattctt tttgtaatcg gagaatttct
     3961 ttttcattca actgattgat ttccttccca tctttcttct ttgaatttgt ttatttcaag
     4021 ttggttattt aaagtttcgt tgtctatttt ttctttatac taacagacct ttctaaaaat
     4081 gaaaatggga agcgatatga ttttagtttt ctgaaaattt ttcctttttt tcaatcgttt
     4141 atgctaagat aaggaagaca aaaatattca taaaaggtga tgaaaaaaat gcagacttcc
     4201 tttcacgagg ggcgtacaat aggacttttg ctgacattta tcggaggagc catggacagt
     4261 tatacttata tccaatatgg tgcttttgcc tctgctcaaa ctggcaatat cattctatcg
     4321 atcatccaag catttgacgg agaatggcta agtgttggta aaaaagtatt gtctaccctt
     4381 ttctttttct tagggattct tctaacaaag tatctaattg attattttag aaaaaaagaa
     4441 aaacatttct ggcgcttgtt tgttctatat tatgaagcag tgattttttt ctttgttagt
     4501 ttgacacccg tccatgagca tcccgcatta gtaacgattt tgattgcttt tactgcatcg
     4561 atccagtggg tagcttttga taaaatcaac gggcgtgcct acactaattt atttacaact
     4621 ggaaatttaa aaggggtagc aacgaactta tatgattatt gtatttctaa agaaaaagca
     4681 gattttgatc gatttttcca ctatctaagt gtcgttcttg cctttatcgc aggagctatt
     4741 gtccttgttt tctgtcatca tctgtttggc gcccattcga ttttgatcgt ttccatttta
     4801 tttttgatca tggccctcac tcagacattt catttatggc aattttatca tacgaatcgg
     4861 ttcactattt gatctcaatg aatcaaaaaa actaagcaag atgctgctca cagagaaata
     4921 tctgcgagtg agcaatttgc ttagttttta tattatttag ttgtaaagat tccctttgct
     4981 ttcgggttat ttgtagtgta gcggatcgac tgggcatctg cctcttctgt cattttcttt
     5041 gcccgcatct tgatttcttg tacgatttgt tctccacatg ccggatcggc tacgaaatca
     5101 tatttgtccc catcgatcac tattttggga catatatcaa actgttcata ccactcttcg
     5161 tatcgtcgat tgagttcttt atagtaggaa tagagttcag gatcttgttc tagctgttca
     5221 tattcccggc ctcttttttt gattctcgct aacatagttt caaaggatac acgaatataa
     5281 accagtagat ctggtttctt ttgtggcgaa atttcttcta actccttcag catcgtttct
     5341 aaaagacctt catactgctt tacttctact tcagaaacac gccctaaatc tgcattcaaa
     5401 tgaaacagca tactatcttc gtagatggag cggtctagta cgttgtcatc atggctgaaa
     5461 gcatctttga ttgccaaaaa gcgtttgttc aaaaagaaaa tctgtaataa aaaagtatac
     5521 ttttctggat tagaataaaa tagcggcaat acttcattat cttccacgtt ctcataaaaa
     5581 ggttttgttt ctaaatcttg ggcaagcatt tcagttagtg tgcttttccc tgcaccgatc
     5641 gttcctgcaa caatgatcac aattatcggc atccttcctg taataaaatc tgttcttcat
     5701 tttactactt catttataga aataaaagcc ttgactttat ctgctcctta aaagctactt
     5761 ctctaaaagt agccaaaagt aactcctatt catgaaaata aaggctgtga caaaagtctt
     5821 gtcgcagccc ttttttcaaa taaacagtat tctcctgcca acacctcggt cacaaggcaa
     5881 tattcataaa acatgaaaag ctgtttttta acattattcc ttgtaactca gagctaaacg
     5941 ggagactcac aacctcttta tgctctcaac attttatctg tttttattct tctattgtac
     6001 ttgatttgcg tacaactaac tgccagtgca gattatgagc ggtatcattg tcttctactg
     6061 cgcatttcat gacacgtatc atcacactat tttcatagat tttttctatt tttcctttta
     6121 ttggtgattc aaataaatca gatttaaatg catatgtttc gccgacttca aacaattgtt
     6181 cattttccat aagaatccct ccatcacctt ttataaggtc agtaattttt taacataaat
     6241 cactcgaaaa agcacaaact tgtcataaaa agtacttttc gtatcaaaac tgtcactttt
     6301 tttggtaagg ttcttggaaa acgaagattt ttttaggaga tcatgacggt tttgatgtca
     6361 aaaccacttt ttatgaaaca aaagacgttt tttatttttt ttagcataaa atttgtggtg
     6421 gaggtacgta tgagatgaaa gataaagttg tgaacaaatt attagaagcc ttattcagcg
     6481 gagacaaatc ggaagaagat atggtgtatg tccaagtaaa atttgtctta gaagttttat
     6541 tgaataatct tggaaaacta gcggtcgttc tagtcttttc tctggtgaca ggctcatggg
     6601 cggaaacagg aatcaccttt ctttcttata tctgcatccg cagatatgct tatggattgc
     6661 attccgactc tgaattcgtt tgtctgctgt ggacactact ttatttatgg ggcgttccct
     6721 tggtgatgaa acatctgcaa ttgactatct ccttcccaat gatggtcttt ttgttaatca
     6781 gctgcttttt gttactgctt cgctatggat caagaggtac agcaatcaat cccattgaac
     6841 ccgagaaacg tcccccatta ttaaagaagg cgatcagcat gtttctaatt ttttctctca
     6901 ttacgttatt tttctcagct tcttactttt ctacctattt gctacttggg attgttttag
     6961 aaatagctac gttgcttcct attacaaact acttgatgaa ttttggagga atctttcatg
     7021 aaaaaaacaa tcattaaaca attaactaac ttatctgact tttttgtatc tggctgctgc
     7081 tttggtatga gttctgaatt caaaatccct gaaaaattaa aaaaataata cgccaattgt
     7141 gctataattt ggtaaaaatc aatgggagct gtactttatg tttttatggt ccatagcgtt
     7201 gcaatatttg atttacacaa tttgtatctt attgattgac catcacatcc gatacaaata
     7261 tctgtttgcg ggagtcattc tgatttcttg tattttgatt ggatgggtct attcgatttt
     7321 agggattttt actgctctaa ttgctatagg aatgctgatg cttacttttt tcttcttttc
     7381 gacttcgcgt caacaattgg tttacgcgct tccttttggg ttattgacgt cgcttttagg
     7441 agatcactta acaagcatcg gcgatttttt tgttttgaaa tcttcggtta ttgaaccagg
     7501 agattcattg cagtattttc acattgtctt atccgctttt ttatctgctt tattagcttt
     7561 ttgtttaaaa aaatcgttag agacttggtt tcacactgtg gaccgccgta tgatcggtac
     7621 gatcgggaca ttgatattat ttacatacta tatcattatt ttttataccc gtttttctgg
     7681 tgaaacacct gatgtcttag ctttgaatac gagttttttc attctatatc tgtgtgttgg
     7741 cctgatcatc ttggtttatt attggaaaat ctctaataag aaattagctg atcagttatt
     7801 agagcaacaa gtcaggatgc aacaggatta tatcaaagat ctagaaaaaa actatcaaga
     7861 cttgagagaa ttcaaacatg attaccaaaa tctgcttttt tctcttcaca gctatatttc
     7921 agaaggcgac ttagaaggat tacggtctta ttatgatcaa acgatattac ctacacgcca
     7981 gatgatgaac ctttttccgg ccaatctaag tatgttggat aatatgaaag ttcctgagat
     8041 ccgtagttta ctgtcattaa aactcatgat ggcacaggaa aaagggttga tcgtccagct
     8101 tcattttcct gagccaatcg aactggatac gaaacatacg gtaaaccttg tacgtatgct
     8161 tggtattatc ttggacaatg cgattgaagg agctagtgct gcaaagaaga aaaaaatcga
     8221 attgaccata ctcaagaaga atcattccgt gatgattcgt gtgatcaata caacaacaaa
     8281 tacattaccg ttaaaccagt tgaaacaaaa agggttctca acaaaaacaa atccaaaaaa
     8341 cgaaggcctt ggcttgttta tcttggatga attgacaaag accaatcctt atcttttcct
     8401 tgaaacatca attgaaaacc agcgtttttc tcaaacgatt tatatacaaa taaaataaag
     8461 gagtaatgat atgattccca tttatatttg tgaagacgat ccaaaacaat tagaagtttt
     8521 atcaacggtg atcaaaaata gaatcatgat tgaaaactta gacagttatg tccatatttc
     8581 tacctctgat ccggaagaac ttctcaatgc agccaagatc agaacttctt cttttggtat
     8641 ttacttttta gatatcgaac tagaagacag tgaaatggat ggtgtagcga tcggtaaatt
     8701 gatccgtgaa ttagatcctt taggaaaaat aatctatgtg acttcccata cggaaatgtc
     8761 gatgaaaatc ttaaaaagca acatcgaacc gactgattat atcgtcaaag aagatcttta
     8821 tgatttaaaa gaaaatgtcg agcatatcct gagcaaaatt ttcgaagatt ttcaaatgtc
     8881 ccaaatccaa aaagatatct tcaaaataga gtttaatgat gagatcaaat ttttaccgat
     8941 caaagacatc caatattttt ctacttcgct aggagcgcca cataaactgg aagtccattt
     9001 gactcatgcc caattacaat tctatgagaa gatcaaagaa atcgagaaaa tgcatcggaa
     9061 ttttatccgt tgtcataaat cctatgttat caatacacaa aatgtgcggt cgattaataa
     9121 aaaaaccaga gaagtcacgt tggcaaacgg cgagaccatc ccagcctcta tccgtggatt
     9181 aaagaaattg atttcataaa aagaaccgaa tagtttggac cgactaaatg accagccgat
     9241 ccaaaccctt cggttctttt ttgcctatca gtgcaaaagc atttcctgtg caatttcacg
     9301 tccatccatt gtatctggat aaaatgttgg ccaattctcc atttctttca gcaatacttc
     9361 ttgactttca tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg
     9421 atcactgaat tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata
     9481 ccttacgcca cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt
     9541 tgaacgtttc gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat
     9601 tgaagcaata tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt
     9661 atcctgattc aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata
     9721 cgtatctact gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc
     9781 gtcttcaaaa taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag
     9841 cgggttttct gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag
     9901 atagttttct ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc
     9961 taaagataca cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc
    10021 aaaataaata tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc
    10081 cgcagacggt tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc
    10141 taacgctaga tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt
    10201 aaaggtttct tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt
    10261 ttttgtccat acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc
    10321 tacctgcttg attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg
    10381 gccttcttcc ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag
    10441 cataccttct gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc
    10501 ggggacccat gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt
    10561 tgccatgtct ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa
    10621 ctctacttct ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt
    10681 cgtcatcacg ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt
    10741 taacatacct gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac
    10801 tcctcttctc atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt
    10861 tacgttttag caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct
    10921 tattttaatt gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct
    10981 atcattttcc ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa
    11041 gagtgaaaaa agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct
    11101 cttactatgt tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga
    11161 tctcgtttat attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt
    11221 ttgcactgat cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt
    11281 cgaggaactt gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa
    11341 gcatatggct tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag
    11401 atttgtttca agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg
    11461 attcatttaa tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta
    11521 ttggaaatta ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag
    11581 ctatttttat tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc
    11641 aaaaatttta tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa
    11701 atttgacaaa gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt
    11761 catttatcct tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta
    11821 gtgttttagc tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg
    11881 ccttctttcc aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg
    11941 tcattgtctg gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg
    12001 gtgatcgttt acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc
    12061 taatgggaat tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg
    12121 ttacttttat tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag
    12181 agtatgaaaa ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat
    12241 cttacgagaa tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag
    12301 caattgcctg gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat
    12361 cgataagaat attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat
    12421 agaaatttgt taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt
    12481 ccggatgatt tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc
    12541 tcagccgaat caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt
    12601 ctacattaat ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc
    12661 ctgcttgttc tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt
    12721 tgatccgacg aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat
    12781 tcaaactttc tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga
    12841 tttcttctcc catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca
    12901 tctctgctcc ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct
    12961 gatcatgatt attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc
    13021 atgtatgaaa tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc
    13081 cattttcata atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac
    13141 taggttctgt atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga
    13201 tgatagtgga atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga
    13261 ttggtttgtc cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt
    13321 ctttaccgat aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa
    13381 aacgaacaac tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt
    13441 gtgtccgatc atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac
    13501 ttccaccaaa ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat
    13561 aatgctggtc ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca
    13621 gcaccatatc gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga
    13681 attcctccat cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt
    13741 tgtctttttg agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat
    13801 tcagatacgg gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt
    13861 ctttgtaaga tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt
    13921 acctccattc aactgattta ttacctacca caacttatat atacaagtta tcatttatta
    13981 ttataactga accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg
    14041 cccaataaaa aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa
    14101 atattcttgt ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc
    14161 tcaaatgtaa aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac
    14221 ggctttttta caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa
    14281 aaaacggcaa aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc
    14341 gctgatttca caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat
    14401 gctttaattt gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg
    14461 ctgataagac ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg
    14521 cttgttgcaa gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta
    14581 aaagagtaaa gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc
    14641 caaggacttt tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat
    14701 tatacaagac gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt
    14761 ctaagttcgc cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg
    14821 tcacagcgtc tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct
    14881 tatctgtacg cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat
    14941 attcagggga catataaata aagctaccta gatagttttt cacgatttct tttacttcga
    15001 ctttccattc tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa
    15061 atagttctgc tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct
    15121 cttcaagatc agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca
    15181 ctgtcagact ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag
    15241 ctaggatctc atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat
    15301 cttccgtgga tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt
    15361 tggctgaaac agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac
    15421 atccagcaat tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt
    15481 aactgatttt ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa
    15541 tgcgtttccc tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat
    15601 ctttgaataa aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt
    15661 gtaaaatcgg cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag
    15721 acagctcaaa aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac
    15781 ctatccctga ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat
    15841 aacctaacgc ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg
    15901 tcgtaaaggt gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg
    15961 cagcgatacg atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat
    16021 tgtataggta tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt
    16081 cctctgcaga ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt
    16141 gagcttcttg ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct
    16201 ggatagttgc tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt
    16261 gcgttgtttg tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg
    16321 ccgatttttt cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct
    16381 tttcttgatt ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta
    16441 cgtttgtaaa atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta
    16501 aatagacaaa ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt
    16561 tgatgaacaa tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat
    16621 ctgaatcaat ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca
    16681 atacaagctg gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct
    16741 tgttattttt gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat
    16801 agatctgttt tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt
    16861 tttgtgtcaa gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat
    16921 agtgatccat cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg
    16981 ttcctagcat gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc
    17041 gaaaacctgc tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat
    17101 caatggatga aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa
    17161 tgactctatc agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc
    17221 cgtgttgtct gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca
    17281 gcgcgcctgt cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa
    17341 ttgaaaccct ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag
    17401 ctaggcccac ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg
    17461 ccaattccac attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga
    17521 accctacatc tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg
    17581 tgtcatcaat aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa
    17641 tcgttgactt tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg
    17701 aaaaagaaat atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt
    17761 cattttttac ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat
    17821 atctatgcat cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac
    17881 taaagaaagg aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa
    17941 aaaacattgt tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt
    18001 gatttatcaa aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt
    18061 acactctctc cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca
    18121 ttgaaacgta ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat
    18181 ttgagtcaac aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat
    18241 ttacttttga aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat
    18301 ccaagtgctt tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact
    18361 atcattttaa gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt
    18421 caacacgggt accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc
    18481 catcaattgt tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa
    18541 ataatcgaag ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc
    18601 tttttgattt catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa
    18661 aaaagatcac aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct
    18721 tgatattaaa tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg
    18781 ccaaagaaga attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa
    18841 cgaaaacaat tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc
    18901 gaggaattga ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt
    18961 ctgatcatca aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat
    19021 tcattatcgc gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag
    19081 gccggccctt cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa
    19141 cagacgattt tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa
    19201 cgatcgccct tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt
    19261 ttaaatctgc tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac
    19321 agttctcaaa acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg
    19381 gtgtgtttgc tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg
    19441 gaaaaagcat ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt
    19501 atatcaagcc gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg
    19561 ccaagttatt gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac
    19621 ctgtacgatt agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta
    19681 tatttttatc gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc
    19741 aaaataaatg agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat
    19801 taatatggac actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc
    19861 gcatgagatt tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg
    19921 ttcaggcgca cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt
    19981 attagatcta ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc
    20041 cacaaccgga caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca
    20101 tgcaggggca aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac
    20161 aaacagtgac tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt
    20221 ttctattgca aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac
    20281 gattcctact gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga
    20341 aatcattaca ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact
    20401 ccatgaatta ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca
    20461 tacagaaaaa gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc
    20521 cgcaggtgat acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt
    20581 gaaagaagcg atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc
    20641 gcaaccttct atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg
    20701 agaggaagaa acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat
    20761 cgcacaaatg gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt
    20821 tggtactgaa aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt
    20881 agaaaacgtt ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac
    20941 aatgaacccg gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt
    21001 tatcccacat gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg
    21061 cgaaatgaca ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg
    21121 acaatgaatc ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg
    21181 ggaggaaaag acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg
    21241 aaaaaacagc cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat
    21301 ttcattttac tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac
    21361 aaggaattga cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa
    21421 aaactatttg gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct
    21481 ggtatattaa aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc
    21541 ggaacttatc atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt
    21601 cttgcttttt ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct
    21661 gcagcactga ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact
    21721 gattttctgg ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt
    21781 ttaggtattt tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat
    21841 atgcaattag tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg
    21901 gtattcgggc ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg
    21961 atggatatta acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta
    22021 atatttggta tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg
    22081 ttcgaccgaa tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta
    22141 ggtgtatttc tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct
    22201 tcgattttac ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa
    22261 aaaccatttg ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc
    22321 caagtgaaag cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc
    22381 ggtacttttt tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact
    22441 actctcgtta gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact
    22501 gaagtatgag atgtttatca catatttcag tctttttctt tttaggatca ttatttttat
    22561 aaatttttgt gatactttcc aaactttggt ataatttaca tatgcataac catttttatc
    22621 cccacttgtt tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa
    22681 cgaatgaatg ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta
    22741 tgaaaagaaa aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga
    22801 ataagacaga aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga
    22861 tacgtttgct agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat
    22921 ttgccgtaga taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct
    22981 cagctactag tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa
    23041 caacaggatc gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc
    23101 cagccgagac atattccctt acaattgctc cttctttgat tggtaagatc caactgactg
    23161 cttcgacagc ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag
    23221 aaggtttatt agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa
    23281 ttgcttcagt cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag
    23341 taggtacgaa caatttatct attactgcag aatttaaagc tggaccggat acttcaacag
    23401 agaaaccaac tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca
    23461 aaccagatgt gggtaataac aacaataata atggttcaaa taattcaaac aataataaca
    23521 ataattcgaa cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg
    23581 gaaatactca gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca
    23641 atgcttcatc tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt
    23701 cttctgattt tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg
    23761 tccaacaagc aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag
    23821 caaaaggtcc aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa
    23881 ccggtcatta tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag
    23941 taagtcaggc ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc
    24001 atgtaggtat ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag
    24061 ttcgagtaac ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag
    24121 gtttacctgc tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg
    24181 gctcgttcca tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg
    24241 atgcacagga aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta
    24301 tccttgaaag tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg
    24361 gtatcaaagg atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt
    24421 ccggacaaag ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt
    24481 tggaagacta tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac
    24541 caacttggaa gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt
    24601 atgctacaga tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga
    24661 caaaatatga tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc
    24721 tcaatatgtc taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca
    24781 caggattaga attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg
    24841 cttcaatctg ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc
    24901 gtacagttaa agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct
    24961 ggcttgatct gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac
    25021 tcccttttta atttattcta tttctttata tttattttgt attccgttac aaactcgcgt
    25081 acttggttag gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt
    25141 tcttgagtct caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt
    25201 ccatcttctc caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg
    25261 atcgatactt tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt
    25321 ttcaacaaaa atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca
    25381 aagacctcac ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact
    25441 acttctcctg taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa
    25501 agtatatgtt cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt
    25561 gtaggattga atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca
    25621 tcgttcgtca gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata
    25681 tctgggctag tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa
    25741 tgccactgct tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg
    25801 tctgttttta cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca
    25861 gccatacgtc cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca
    25921 tcaaaaccta agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc
    25981 cgtgccccaa gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag
    26041 tgatagtttt cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt
    26101 tcatttgcac tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt
    26161 aacgagaaaa aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa
    26221 tattgtcaca acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata
    26281 agcccaacca aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat
    26341 gcaggacagc tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc
    26401 agtacatagt agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg
    26461 tatggatttt gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct
    26521 aagattcctg gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac
    26581 ccgcgtcgtg ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct
    26641 gacaagatct tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag
    26701 aatcccataa attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc
    26761 tggttgctgt actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt
    26821 gtttcgattg gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat
    26881 tgtggcaagt tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc
    26941 tgttccggca attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta
    27001 gcaggaactt tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc
    27061 aatggttgag ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga
    27121 gtataagaag cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga
    27181 ggattattca tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt
    27241 ttaaaaccta atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt
    27301 gagagcattt gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc
    27361 aattttttca atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca
    27421 gaatggaaaa tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag
    27481 acttgatagg tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc
    27541 agtactactt caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc
    27601 gcacgaatca atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct
    27661 aataaaaaca aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt
    27721 gtaaaatgcg aaatattgag atctaactga tccactaata atcggtggac gatacttgtc
    27781 aatcctacac cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca
    27841 gaaaaaatca tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag
    27901 tctttttgcc gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg
    27961 atccctagta aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta
    28021 cttttcaaaa aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc
    28081 ataactaatc ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt
    28141 gataatttcc aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag
    28201 tatttctcga tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc
    28261 gccatccttg atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac
    28321 aagaagaatc gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg
    28381 ggcacttttg gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt
    28441 gatcaatgca cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata
    28501 gctttccaaa atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat
    28561 ttcttttggt ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa
    28621 gctttctaat aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc
    28681 tagttgattt ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt
    28741 tggtttatct aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc
    28801 catgatccca ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt
    28861 ctttttctca ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt
    28921 catttctttc cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt
    28981 tcgacttctt catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct
    29041 cctttacgaa gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct
    29101 ttcgtatagc caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt
    29161 gaaactgttt ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact
    29221 acgtaatctg cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc
    29281 agaagttttt tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc
    29341 gaacaattct gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa
    29401 acaactaaaa aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca
    29461 aaaagtatgt cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt
    29521 gtctgtaatg attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa
    29581 attgatttct tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc
    29641 ttcaacaatg gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc
    29701 actagcgatt tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc
    29761 tgctactgct aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt
    29821 tcctcgaagc gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg
    29881 ccgaatggct gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa
    29941 tgcctttgct gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc
    30001 atccaaatca ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt
    30061 cattttttgt gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata
    30121 ttgtccaaaa aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa
    30181 caacaataac gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg
    30241 ccacatacct aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat
    30301 ccctgcataa ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct
    30361 ttatccttca ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt
    30421 acaccttcta gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct
    30481 ccggctgcct gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa
    30541 tagtcttcta ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca
    30601 gatttaaaga taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc
    30661 ggtaaagaat aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg
    30721 tacacttgct ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct
    30781 ttgtctggat catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt
    30841 tctgttttta acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt
    30901 tctttttaga tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac
    30961 agcaaagatt ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc
    31021 aatctgtttc attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga
    31081 tctttagctt tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt
    31141 caatataaga ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag
    31201 ataaattgcc accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat
    31261 ggaagttgct gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa
    31321 gtgagcctaa ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc
    31381 agccatcatg tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac
    31441 tattattccc ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga
    31501 tttgtgcact tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca
    31561 cagtcaacac gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt
    31621 ttatctcaaa atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga
    31681 ttttgtttgc atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat
    31741 tatcccaagt cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac
    31801 cagtaagcat cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc
    31861 tccttcaaac gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt
    31921 taacaaagtt catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa
    31981 taaatactgc gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa
    32041 tcgattgtcc aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac
    32101 tgatacataa aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa
    32161 taaccaatat aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa
    32221 tgattaacca aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt
    32281 gttcttcttc tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat
    32341 attcatcaat gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact
    32401 ggatcaaatc aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca
    32461 aatattcatt catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc
    32521 cattcatcaa taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga
    32581 cggtttctgc cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt
    32641 ctcaaaaccg gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt
    32701 aaggcatacc catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga
    32761 acagttggaa tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt
    32821 caatctatat tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa
    32881 ctgactaaaa gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt
    32941 ataacagtgg cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg
    33001 caggtatcat gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga
    33061 ccatcaagaa tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat
    33121 aaataaattt tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca
    33181 cagaaacgac ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag
    33241 atttagaaat caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc
    33301 ccgtaccact tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt
    33361 gggtcatacc aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag
    33421 cccccttatt tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta
    33481 aagcaaaaat aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca
    33541 tcgaacacac accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc
    33601 ctactaatcc gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag
    33661 ttggcatcaa aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt
    33721 aattattgct ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt
    33781 ttttgtgtta aagtattctt tattggaata agtaggagga atatgatgga tatccataaa
    33841 ttcgaagttt ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa
    33901 ttcaccaccc aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc
    33961 aaattattcg aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc
    34021 ccatatgtaa aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat
    34081 acgaaagaca acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa
    34141 ggtttttcca aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa
    34201 gaagtggaaa gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac
    34261 tttgctagaa gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac
    34321 gattttgtag cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta
    34381 agcgaactga aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct
    34441 gtactagatc tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga
    34501 atcgacttga tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa
    34561 acagtcaaga atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag
    34621 agttatcttg catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg
    34681 gagtatttaa atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa
    34741 aaaagagagc gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac
    34801 acctctaaca tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt
    34861 aagaacattt ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat
    34921 ttttaggggt tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct
    34981 actccttcga tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc
    35041 agtgaaagtc ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt
    35101 tcaaacattc gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt
    35161 acaatctctg tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac
    35221 tttatggcgt tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg
    35281 taatttactg cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga
    35341 aaaaaaacga ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg
    35401 atacgctggt tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc
    35461 gtgatacttt cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg
    35521 gcttgtaaca attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat
    35581 acactggaca caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg
    35641 ttgaaatctt tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc
    35701 gaaaaatctc ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg
    35761 atacgccgcc caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag
    35821 acgaataacg ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga
    35881 gacaataaga aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca
    35941 atactagcaa aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg
    36001 ccttatagcc tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg
    36061 tccttaaacg tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc
    36121 aaatatcatc catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat
    36181 acagaagcag aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat
    36241 aagcatctag ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg
    36301 aacgtcgaag caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca
    36361 tataatcatc tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg
    36421 taatcattag aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc
    36481 catccatttt cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt
    36541 ttttcagcgc atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca
    36601 cagaacggta aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg
    36661 atctccctct gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac
    36721 atttatttcg tatactacga tcattgttct gatatactta ttagtatacc gaatatttca
    36781 atacgggaga agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg
    36841 cgaaaccacc accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt
    36901 agcgatttta ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact
    36961 agataattat gattccggtg atatggtaat caacggaaag tctactaaag actttaagga
    37021 cagtgactgg gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt
    37081 gattggtcat ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc
    37141 taaagacgaa aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca
    37201 tatgcataaa aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg
    37261 agcactagcc aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac
    37321 agaaacaagt atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat
    37381 catggtcaca cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc
    37441 tgatggtaag atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt
    37501 taacttacgc agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat
    37561 ccgaacaaaa aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg
    37621 aatcgcgatc gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc
    37681 tgaaacgatg gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga
    37741 cgatgccgga ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa
    37801 agtcagcgaa gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta
    37861 cgtaacagac atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat
    37921 caatctttta cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc
    37981 tgatacggaa tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc
    38041 aagtttccct acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt
    38101 acttgccgga agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa
    38161 taatactaat atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact
    38221 tgattttgat gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac
    38281 aaaacttcca actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc
    38341 ttctgatgaa ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct
    38401 cttatctcca ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa
    38461 agaatcagaa atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa
    38521 agttgacgaa aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc
    38581 aagtatcatg atttatccga acaattttga agacaaagaa aagatcttgg attatctaga
    38641 tgattacaat aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac
    38701 gatgactgaa ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc
    38761 agggatctct ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat
    38821 tgaacggaca aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac
    38881 tcgcgtattt gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt
    38941 tattgcatgg ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa
    39001 aaatgttgct caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt
    39061 gacgatgctt ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc
    39121 tttgcgtgca gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa
    39181 atagtagaaa acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt
    39241 taattatttt ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg
    39301 tatacctgtc ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc
    39361 caatccacat attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc
    39421 caaattgcaa ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc
    39481 cgttttcaaa tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa
    39541 atgactcctc catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag
    39601 tgataagtat atcatagttt attctattat caaaaaaacg catccattcc attgtagaat
    39661 actttaccga acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa
    39721 gacaactaag gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga
    39781 agaactaaaa acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc
    39841 agaattatct ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca
    39901 agaaggacca gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat
    39961 cggaacaatg gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact
    40021 tggtcgtttc ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc
    40081 agacgctcca gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa
    40141 cattttgcct gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt
    40201 agtcttagga tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct
    40261 agaagataaa gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt
    40321 ccaaaaacaa ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga
    40381 tccagcaatt gccattggca cgactttcga accacctacc actccacttg gctacaatga
    40441 actatggtgt gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt
    40501 cgatgaagtg ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga
    40561 aacaatgcaa gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta
    40621 tactggggat gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa
    40681 agacaatcca atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg
    40741 tattcctaca gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt
    40801 aaatgtctac aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa
    40861 agaaaacgaa gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt
    40921 caaagaattg aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga
    40981 tgtagtttgg actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg
    41041 aatgaggaac catccattgg acccatcaga attaccagaa tataatcctg gctctattcg
    41101 tttccgtggg ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga
    41161 acattttgta cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg
    41221 aatgatctgc aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac
    41281 aacggaagaa aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca
    41341 acctagtgaa gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat
    41401 gggcaaaaca gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt
    41461 ttgattacca ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga
    41521 ccgatggaat gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt
    41581 taggcgacaa cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag
    41641 tcattgttcc tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag
    41701 cacgactagg tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga
    41761 tccaagatct tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg
    41821 agcattcaaa acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc
    41881 aaactaggtt tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact
    41941 ttaacaggcg gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag
    42001 atgcaaactg tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc
    42061 gaaaaaatag ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt
    42121 atccatgttg accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag
    42181 ttaggttaca agattttaga ttggttggaa aaaatagatt tccatatcaa agaaccagtt
    42241 tattataaag atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac
    42301 ctgagtatga gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa
    42361 taccgaggga cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt
    42421 tcgagtagta agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt
    42481 actaccgaga aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc
    42541 acaacctctt ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga
    42601 tgtttttacg aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt
    42661 tcacgcccat attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt
    42721 taactcttcc agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc
    42781 ccttgccgac ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat
    42841 ttctcctatt ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag
    42901 tcttgtcgca gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    42961 gtcacaatag tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag
    43021 ttaaacggca ggctcacaac ctcttcct
//
LOCUS       DACEDM010000013        41730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEDM010000013 DACEDM010000000
VERSION     DACEDM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.013"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            287..1156
                     /locus_tag="IWO50_000682"
     CDS             287..1156
                     /locus_tag="IWO50_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0357825.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1365..2390
                     /locus_tag="IWO50_000683"
     CDS             1365..2390
                     /locus_tag="IWO50_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0357826.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2429..3379
                     /gene="manA"
                     /locus_tag="IWO50_000684"
     CDS             2429..3379
                     /gene="manA"
                     /locus_tag="IWO50_000684"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0357827.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3473..5176
                     /locus_tag="IWO50_000685"
     CDS             3473..5176
                     /locus_tag="IWO50_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0357828.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5178..7211
                     /locus_tag="IWO50_000686"
     CDS             5178..7211
                     /locus_tag="IWO50_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0357829.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7222..7662
                     /locus_tag="IWO50_000687"
     CDS             7222..7662
                     /locus_tag="IWO50_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0357830.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7862..8989)
                     /locus_tag="IWO50_000688"
     CDS             complement(7862..8989)
                     /locus_tag="IWO50_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0357831.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8992..9669)
                     /locus_tag="IWO50_000689"
     CDS             complement(8992..9669)
                     /locus_tag="IWO50_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357832.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9933..11699
                     /locus_tag="IWO50_000690"
     CDS             9933..11699
                     /locus_tag="IWO50_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0357833.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11714..12151
                     /locus_tag="IWO50_000691"
     CDS             11714..12151
                     /locus_tag="IWO50_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0357834.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12164..13315
                     /locus_tag="IWO50_000692"
     CDS             12164..13315
                     /locus_tag="IWO50_000692"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0357835.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13377..14075)
                     /locus_tag="IWO50_000693"
     CDS             complement(13377..14075)
                     /locus_tag="IWO50_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0357836.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14068..15420)
                     /locus_tag="IWO50_000694"
     CDS             complement(14068..15420)
                     /locus_tag="IWO50_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0357837.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15643..17043)
                     /locus_tag="IWO50_000695"
     CDS             complement(15643..17043)
                     /locus_tag="IWO50_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0357838.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17204..17941
                     /locus_tag="IWO50_000696"
     CDS             17204..17941
                     /locus_tag="IWO50_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0357839.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18066..18545
                     /locus_tag="IWO50_000697"
     CDS             18066..18545
                     /locus_tag="IWO50_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0357840.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18542..19309
                     /locus_tag="IWO50_000698"
     CDS             18542..19309
                     /locus_tag="IWO50_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357841.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19539..20108
                     /gene="lepB"
                     /locus_tag="IWO50_000699"
     CDS             19539..20108
                     /gene="lepB"
                     /locus_tag="IWO50_000699"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0357842.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20212..20814)
                     /locus_tag="IWO50_000700"
     CDS             complement(20212..20814)
                     /locus_tag="IWO50_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0357843.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20920..21389
                     /locus_tag="IWO50_000701"
                     /pseudo
     CDS             20920..21389
                     /locus_tag="IWO50_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21495..22079
                     /locus_tag="IWO50_000702"
     CDS             21495..22079
                     /locus_tag="IWO50_000702"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0357844.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22082..23155
                     /gene="prfA"
                     /locus_tag="IWO50_000703"
     CDS             22082..23155
                     /gene="prfA"
                     /locus_tag="IWO50_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0357845.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23148..23987
                     /gene="prmC"
                     /locus_tag="IWO50_000704"
     CDS             23148..23987
                     /gene="prmC"
                     /locus_tag="IWO50_000704"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0357846.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23980..24159
                     /locus_tag="IWO50_000705"
     CDS             23980..24159
                     /locus_tag="IWO50_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357847.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24181..25206
                     /locus_tag="IWO50_000706"
     CDS             24181..25206
                     /locus_tag="IWO50_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0357848.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25381..26625
                     /locus_tag="IWO50_000707"
     CDS             25381..26625
                     /locus_tag="IWO50_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0357849.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26778..27725
                     /locus_tag="IWO50_000708"
     CDS             26778..27725
                     /locus_tag="IWO50_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0357850.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27859..28488
                     /gene="upp"
                     /locus_tag="IWO50_000709"
     CDS             27859..28488
                     /gene="upp"
                     /locus_tag="IWO50_000709"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0357851.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28627..28869)
                     /locus_tag="IWO50_000710"
     CDS             complement(28627..28869)
                     /locus_tag="IWO50_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357852.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28990..29062
                     /locus_tag="IWO50_000711"
     tRNA            28990..29062
                     /locus_tag="IWO50_000711"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29023..29025,aa:Thr,seq:cgt)
     gene            complement(29181..29819)
                     /locus_tag="IWO50_000712"
     CDS             complement(29181..29819)
                     /locus_tag="IWO50_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0357853.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29942..30574)
                     /locus_tag="IWO50_000713"
     CDS             complement(29942..30574)
                     /locus_tag="IWO50_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0357854.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30925..31224
                     /locus_tag="IWO50_000714"
     CDS             30925..31224
                     /locus_tag="IWO50_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0357855.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31719..33884
                     /gene="acm"
                     /locus_tag="IWO50_000715"
     CDS             31719..33884
                     /gene="acm"
                     /locus_tag="IWO50_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0357856.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(34156..34641)
                     /locus_tag="IWO50_000716"
     CDS             complement(34156..34641)
                     /locus_tag="IWO50_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0357857.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            35121..35762
                     /locus_tag="IWO50_000717"
     CDS             35121..35762
                     /locus_tag="IWO50_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0357858.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            35781..36416
                     /locus_tag="IWO50_000718"
     CDS             35781..36416
                     /locus_tag="IWO50_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357859.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            36434..37249
                     /locus_tag="IWO50_000719"
     CDS             36434..37249
                     /locus_tag="IWO50_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0357860.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            37568..39004
                     /locus_tag="IWO50_000720"
     CDS             37568..39004
                     /locus_tag="IWO50_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0357861.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            39193..41640
                     /locus_tag="IWO50_000721"
     CDS             39193..41640
                     /locus_tag="IWO50_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0357862.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT        14324 a         7163 c         8533 g        11710 t
ORIGIN      
        1 tttttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
       61 accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact tgatgcagaa
      121 cagctttttc acaacctctt tttataagag tttttactca ttaatttaca aaaaacaagc
      181 aaaatgcgaa ttttctcaca ttttttttaa caaaatcaaa gttattgctt gaaagaagaa
      241 gaatccatcg ttaaaataaa gcttgacgta gaggggagca agaaatatga aagattttca
      301 attgggggct gccacctatt taaaaagtgt agccattcgg gtgaaagacc gagataaaat
      361 gattgatttt tacaagaacg ttgttggctt tgatttgaaa agagaagaaa acgaattagc
      421 cattttagga acagaagaag ctggaagtga aatgttgctg ctggaagaaa gtcctagagc
      481 gaatgatcac atgggggtga tcaaaaagct gaaccgtttt tcattgatta tccctagcac
      541 agaagaaatg gcagatatcc tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc
      601 tttagaagat catggacgta tggggatttt gctcgtagac ccagaagaga atgaattgga
      661 aatttaccat gaagagaata agaacgacca aagagaaatt ccagaaacaa tggatcaaga
      721 agcattatta agaaaatctg aagaaaaatt cagtaaacta tcaacaggtt cttattttga
      781 aaaaatccat ttaaatgtta ctaacttaac aaaagaacgc caatttctta atcatgtact
      841 tggtttgaag gtacataatg aagaagaggg catccatgta ttgaatgaag gagatttcca
      901 tgttggtttg acagaagcta gcggaggcgc aattgatttg cctacagatg aagtgattgg
      961 acttgatttt ctgaaattca tgatcgatac tgaaagcatg gaaggactga ttgctcactt
     1021 gcagcaacta tcagttgatt tcttcttaga caaaaagcac acagtactca caatctatga
     1081 tgctatagga atcgaatggt ggttcgtcag tgagccaaaa gaaaagaata aaactgacat
     1141 tcgatgcgac gcttaacttt ttctttctta acgattgtcg catgtctcgt cattttatga
     1201 acggtcatac tatcgcttat tctagagata gaaagagaaa atagtatacc gggggaaact
     1261 gcgcggtttt tcgcgcagtt tttttgtgta aaaaaggttg attcctcata gattaagctt
     1321 taagcttgga ctgtaagaat ctgatagaaa agcaggagaa aaagatgtat ccacacacga
     1381 aacagcttat tttgaaaaag atggaagaag gattttatcc aggtgtagca gtcgccttca
     1441 taaaagaaaa tcaagtagaa acatttacag ccggctttgc ccaagtcgtt cctgaaaagc
     1501 agctgatgac agaagatctg ttgtttgatg cagcctcact gacgaaagtc gttgcaacca
     1561 cttcattagt tttacgtcta ttagaagatg gaaagataga actcgatcaa ccgcttcata
     1621 aatatcttcc tgaatttgaa aatcagacaa tcactttgcg tcatttactg acacatactt
     1681 ctgatatcaa aacatggata ccaaacagag ataagctatc tcaggaagaa ttgatcgccg
     1741 cttatttgaa gctagaacca ggtgagcaac taggaaaaga agtaaaatat acagatgccg
     1801 gtacgattct tcttgggttg atgctagagg aaatttacca agaaactgca gtggaaatct
     1861 ttcaagaaca agtattggaa ccattaggaa tgatgaacag cttatttctt ccttttcctt
     1921 ccaaaaaaat cgttcccaca gaacaactgc cttcgggtga ggttttgcgt ggacaaactc
     1981 atgatccgaa agcaagagtt ttggctgaac atgcaggtaa cgcgggtctc tttattgatt
     2041 tgaaagacag cattactttt gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc
     2101 gttttttaga agaagagacg attgattctt tactgcaaga tcaaacaccg aaaaaaaatg
     2161 gctatcgttc tttaggatgg gacttaaaaa aggatgtaga agacggtcat gcgattcttt
     2221 ttcatactgg ttatacaggt acgtttttct tgattgatat aaaaacacaa gaagcgttga
     2281 tatttttgtc aaatcgcgta catcctgtcg atcatcgtgc ggagtatctc atatgtcgag
     2341 atgaaattgt tgaaaacttt ttgaaagaga aagcggtatt accgaaataa tgctataata
     2401 gctatgaaaa atgagaggag tttgaattat gcaggaacct atgtttttaa aaccagtttt
     2461 tcaagaaaaa atttggggag gaagtcgttt acgttcagtc tttggttttg atatccctaa
     2521 cgataaaatc ggagaagatt gggcgatcag tgcgcatcct catggtgtca gtgtagtaga
     2581 aaatggcgaa ttcaaaggga aaagattaga tgaactatgg tcagaacaca aggaattgtt
     2641 tggccatcct tcagaaccag tgtttccatt attgatcaag atcttagacg cagaagatga
     2701 tctatctgta caagtacatc cagatgatgc ctatgggatg aaacatgaag gtgaattagg
     2761 aaaaactgaa tgctggtata tcatcgatgc tgaacctggt gcagaaatca tctatggtca
     2821 tcatgcaaag acaagagaag aactagctga gatgatcaaa gatgggcgct gggatgacct
     2881 tttgaagaaa gtaccagtta aaaaaggtga tttcttttat gtaccaagcg gaacgatcca
     2941 tgctattggt aaaggaatca tgattttaga aacacagcaa agttctgaca cgacttaccg
     3001 cgtgtatgac tacgacagaa aagatgcgca aggacaaaca agagagctgc atatccaaca
     3061 atcgatcgat gtaacaactg tccctgcgaa aacaccagaa ttacaaatca aagaagtccg
     3121 aaaagggaat tcatcgatcg tcacttattt agaaactgag tttttcaatg tatatgagtg
     3181 ggatattaaa ggaatcacat ctttcaaaaa acaagcccct tatacattga tgacggtaat
     3241 cgaaggagca ggagaccttg tcgttgacgg aaaaacatat ccattagaaa aaggaacaag
     3301 ctgtattatt ccaaatggcg tttctgagtg ggcggtccaa ggtgaactag caatcattgc
     3361 gtctgaacca ggcaaatagt tcccagatag ctgaaagcag cagacggttt ttcgtttgct
     3421 gctttttcta cataaaatcc gtccatcaat aaatagaagt ctggtgaaaa agatggaata
     3481 caaatggaaa gcaaacaaca gcagtcgcgt gtctatgaaa caactgagaa aagctggaca
     3541 tcacttaaat gaaatgatca tgccaaatct aagtctgttt atcgcttggg gaatcttgtc
     3601 attagcaaca aaaggtgtgt ctggcgattt gcacaccaca ttagaagagg tatgccattg
     3661 gatgatccag ctgcttttac ccatcttgat cagttattca ggcgggaaat cggtaggggg
     3721 acaaaaaggg gcagtcgccg gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc
     3781 tccacaaatc gtaggagcga tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt
     3841 tgtttctaga ttccatgtga aattcaaggc tggatatgaa atgcttgtca gcaacctact
     3901 agtaggactg actggtagcg ttatttgtat aattggaatc gtttttattg aaccagtttt
     3961 aaatagtgtt cacttgttgc tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt
     4021 accacttgtc agtctgatct tagaacctct aaaagtacta tttttcaata atgcaatcaa
     4081 tcatggtgta ttgacacctt taggaatcga attctcacaa acagtcgggt actcttcctt
     4141 atttttgatg gaggcaaatc ctggacctgg tttagggatc ttactttcta tcctttgttt
     4201 tgcagaaaaa caggaaaaag taaatgcgag tggagcattg atgatccatg cgattggcgg
     4261 gattcacgaa atctattttc catttgtctt gctgcgaccg tatctatttt tagctgtaat
     4321 ggctggtggg gcaagcggca cgttgatatt tcaatggtta gatacgggat tagctgcacc
     4381 tgtatcgcct gggtcactac tcgtgatttt tgccaatgcg ccttatccac aactagctgg
     4441 ggtaagtata gggatagcaa tcagtacatt tgtgaccttc atttgtgcag cgatactatt
     4501 gaaattacca aataaagaaa aagagaacaa aacggtcaaa gaaaggggag aaatcttgga
     4561 accatctatc attcattccg tcatttttgc ttgcgactca ggtatgggat caagtgctat
     4621 gggagccgcc ctgctgaaaa agaaattagc agcagaaaaa atcgatcttt ctgtcggata
     4681 cagctctatc tatgaattga ataaccaagt aaatcagctg gttatcgtgc aacatgaact
     4741 agcgggtctt gcaagagaaa aagtacctca aagtcaaatc tatgtactgg atcacttttt
     4801 ggaaacagat atcttgctgt cacgaataaa agaagcgatc caatttccaa agcgggaaga
     4861 aaaaaaagag atagcagata tcacagaaca aaaaacgtcg cctgctaata ttgtattttt
     4921 gtataaaaac aacagacgcg gctcacaaac tatgggcatg acgattttaa gtcaattagc
     4981 aaaaaaagcg aaaaaacagt ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca
     5041 agaagaaacg atttatatcg cacaaagaga actagctgta actgaacagc tagaagaaaa
     5101 gatccctcgg ctagttctag ttgaacattg ggtagctacg aatgaatacg aaaaactcgt
     5161 gaagggagaa atataagatg tatttatcgc ccagagaaaa acaattatta gcagaatttc
     5221 tcagtagccc gtcacctgta tctatccaaa aaatgatgaa tttattgaag gtcagtaaac
     5281 ggactattta ccgcgaatta gataatcttg aagtctcatt gaaatcggta aatgcttctt
     5341 tgaaaaaagc agctagaggc tcatttgtca ttgaggcatc agaagaagta ttagagcaat
     5401 taaaagaaaa tacagatggg aaaagtaatg acttatccac tgctaaacga caacacgcga
     5461 ttttagccga gttgttactg acgacagagc cgttgagtct tgctcatttc atggaatcat
     5521 atcgcatcag taataccact ttttatgcag atatcaaaca gttggaagaa agtattcgcc
     5581 aactgccttt ggaaatcatt cggaaccagg gatacgagat cgaaggaccg gaaaaatacc
     5641 gccggcttct gacagcgaat gtattagaat tagaaatcaa tgaatatgaa ctttttcata
     5701 gtatttcttt cgatgcatca ctgaattatt tttttcgatt cgttgatccg caacatctat
     5761 ctcttgcacg caaagtaatc ggggaagaat tgatccaaaa aaagaccaat ctaagtgata
     5821 gaaagcttga acatttggtt ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc
     5881 atttactgac aaatgagaca tataatagtc tggcaaacaa agaattacta cagacagcta
     5941 aacgattgtt ttcacggatc gcttctgaga caaaacaact ttatccagta aatgagatcg
     6001 tcttttttgc aggattatta aatgacttca cgaactcatt tgatcagaat ttctttgaag
     6061 agacgttcga tacacagctt gcttatttag taaaacaact gattcaagaa gtaagtgaag
     6121 agacgaatgt ccatttttat gaagatgaaa ctttgtacaa aatgctgttg actcatttat
     6181 ctggtgtgtt ttcaagagcc gttttacaag aagagacact gaccaatccg attttagaaa
     6241 aaatcatggt tcaatacgag gaagttgcga atgcaatccg taaagcagta gagcggattt
     6301 ttcaagaaaa aaaattatcc gaagaagaga tcgcttatat ggtccttcat tttgcgaatt
     6361 cattggaacg aagtccgaaa gaaagcaata tcagtattgc aggattttcg ccaagtggat
     6421 tagcttctac tagcatgttg gaactacgat tgaaaaggta ttttcccttc atccaacaaa
     6481 ttcatttcta tcggattgct gatttaaaaa aaatcgattt acaagaagaa tatgatgttg
     6541 ttatttctac atctatttta ccaggatacg gtggaaaata tattttagtc tccccgctac
     6601 tgctagaaga tgaagtcaag cagctaaaag aagctttttc gcatattgag aagaaaaaaa
     6661 ggcaaaaaat tacgtataac agcaataacg agtggatgat agaagatact tatgaggaaa
     6721 caatgcattt catggatcaa atcaatcaat tactcggtcg tttttttatc caagaactgg
     6781 ataatcctga gacgatttcc gaaacgattc gcagcttatt tccttatttt cctgaaggac
     6841 tagtaacgga tgaagaggtg gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta
     6901 tcggtattcc tcatacaaat atggggttat tccatacatc cagctcattg atttctaaac
     6961 cgatcttttg tattttcaat ttgaaacatc cgttgaccat tgaagggatg gatagacagc
     7021 cagtcgattt gtcccgtatg ttagttatgc tggcgccttc gccaatccaa gaaaaagacg
     7081 ggattctttt aggaaagatc agtggagcaa tcatcatgaa tgacttgaac accgagatct
     7141 tccgctcagg aaataaagaa atcgtttatc agttgttagc gaaaatcctg attgaagaaa
     7201 taaagaaatg aaggtgaaaa gatgatacag ctaccagcag aaaaaatatt gctcgatcaa
     7261 tcctatcaaa attggaaaga agcgttgacc gagatcggcc agtggatgac ccaacaaggg
     7321 ggaatcaaac cggcatatat ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat
     7381 atcgggaatt tcgtagcctt gccacatggg actgacaaag atgaagaagt agtagaagaa
     7441 ggaatctatt tagtccaagt gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa
     7501 atcgcgacac tcttatttgt agtggcttta aaaaaagaac atcagttagc ggtgctccaa
     7561 gatttagcct ttttctgttc agacatcgac caagtaatgg cgctcagtga tgcggaaacc
     7621 attgaagaag tccaaatgat ttttgaacat ggacatatat aaccaatgtt cacagtaaaa
     7681 aaagctgcct tgcatcaagt aataacttca accaagataa aaataagagg agctattttt
     7741 gttgggaggt tcttattact gcggatgcaa gcttttgaaa actatttatt ctagaaaacc
     7801 gtttatttga aataggggac ggtgacaagg tttttgtcac cgcccctatt atttttgtgt
     7861 tttaatagct cttttttata tagatagtgc ccatacctat gaataataaa acgatactcc
     7921 atataccaag tgtcaagata ccattcatga aagtaacctg ttctgaagct agtggcgaga
     7981 aaatatctcc tcctatgatc acattttgaa aatcaacata agaagttggc aagtaatgac
     8041 tgattgattc tatccatcca atcgaagaaa acaagcgata ctctgctaag aaaagacaaa
     8101 gaagtgtacc taaaagattg actagagaat gttcaaataa agcagataga agaaaactga
     8161 ttgtacttaa aaagagcacc cacaacaaca ggaacagaaa gctctggaaa agatactcat
     8221 acatcggcag gattcgtatc tcctgaccaa tgatcgttcc tacaggataa cggaagtctc
     8281 ccaaaccatt tttgattatt actagtatag agacgatcaa aaatggcatc acgagactta
     8341 gcatagctgc accataataa atggtaaaac ggatgctatg tttctttacc atactatccg
     8401 gtaatagatt gactagagag attacctgct ttcttttttc ataagagatg atggaagaaa
     8461 gaaacaaggc ggttatacat aaaatcaaaa aactagaaat cattcctgaa aaaatatttt
     8521 cgtagtaatt tgctaaagag agatgcgctg gcaagtcaaa aatagaatat ctttggatct
     8581 gatgttcttt catataacgg agcaactcga cacgtttttg ttgttcaatg attggaattc
     8641 cttgtctttg accagaaatc agtcgatcca gtatatcttt ttcataatga taagttgctt
     8701 cgaccatttg ctggacattc cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt
     8761 tgcttttctc taaactttcc acaaattctt tctcattctc atcttctttc aacgtttcaa
     8821 tcaaacgttg catctcttca tagttatgat tcagttcatc gaattctgta taagcaagtt
     8881 tatgatcaaa aaaaagtggg aatgcaaaga gcacgaccaa caatagagga aataaaaagt
     8941 ttactttgtt ttttagtaag agattgcgtt caaatgtaag taaagacata gttacacctc
     9001 atataattta cgataaatag ctaaaatctc ttccatattt gaggattctg tgacgatttc
     9061 tccatttttt agaaaacatg ctttagaaca aatcgattgg atattcgcga tagcgtggga
     9121 agaaagaaga atcgtcttcc cctctttctg ccattgtctg atgaggctat tcaagatatc
     9181 aatacttcct gggtctaatc cgttcaacgg ttcatcgaat aacatgacag atgaatcact
     9241 gatcaagtaa gcagcaagca agacatgttg tttcattcct agagacattt ttccaatcgg
     9301 caaagtaagg tactcagtca tcttcaatcg ttggaaaaca gtttggcatt ctactttgct
     9361 ctgccaatag tatttgatca aagtcaaata atcaacagcc gatagatagt cggataactg
     9421 attcgttgat tctagaaaaa agaaagaccg gcgataatct gtataagcag taggagagat
     9481 gttgtgaagg gacaattgat cataagttgt tggcagcagt tgggcaatgc cattcagcaa
     9541 agttgttttg ccaaatccgt tcggggcaat caaagcagct acttctcctg attctactaa
     9601 aagattcaat tgtcgaatca cacgtttttt accacgtttc atgttcatgt ttttgatcag
     9661 taatgtcata gataggcctc caatataaaa tttaataaca taatagaatg gaaaacatct
     9721 aaattaaatg ctaacaaaag ttattgtttg catattataa aaaaattaaa ataacagttt
     9781 tatttaaaat tgaagttttc tgataccgaa atctttttat tttgctgaca tactcaaacc
     9841 tttggcacaa agtttcgtgc caaagtacga ctatttctct ttttagtaag cggatacaat
     9901 tagagtatca aaaggagaga gggagagaca aaatggaacg gacgattccc aaaaacaaca
     9961 tttcgctaaa agcgagggta caaaaattag gcagtacttt atcaagcatg gtcatgccaa
    10021 atattggagc actgattgcc tggggtgtat taacggctct atttatccca gatggttatt
    10081 taccaaatga ggctttcgct accatggtaa gcccaatgct aacttatctg attcctttat
    10141 tgatcggtta tactggcgga aaagtaattg ctggtgaccg cggatcagtt gttggggcaa
    10201 ttgcaacgat gggtgtgatt gtcggaacag atattccaat gatgctaggc gcgatgatca
    10261 tgggacctct tggtggatat gccatcaaga aatttgatca gctattccaa aaacggatca
    10321 aatccggttt tgaaatgcta gtcaataatt tttcagcagg tcttatcggt tttggattag
    10381 cgttattagg tttttctgcg attggtccag ttgtcgatgc attgactcaa gcaatggcaa
    10441 aaggtgtaga gatcatttta tctgcccatt tgattccatt gacaagtatt tttattgaac
    10501 cagcaaaaat cttatttttg aataatgcaa tcaatcatgg gattttgaca ccattgggaa
    10561 ctgaacaagt agcagaggct ggaaaatcta ttctattctt gcttgaagca aatcctggtc
    10621 caggacttgg cgtattactt gcttttactt tgtttggcaa aggagcagca aaatcttcag
    10681 caccaggagc catgatcatt cactttttag gcgggatcca tgaaatctat tttccttacg
    10741 ttatgatgaa accgttatta ttcttatcag ttataggcgg aggaatgtca ggaagtttca
    10801 ttttccaatt gatggatgct ggactaagag cgccagcttc tccagggtcg atcattgcga
    10861 ttctagcgat gacaccgttc aattcttacc ttcctgttat tttgggcgtg attgttgccg
    10921 cagctgtttc gtttggtatt tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg
    10981 aagacttcga aaaatctgtt caagagacaa aggcagctaa gcaagcttcc aaaggtgccc
    11041 aaggtattcc tcaaggagct acattctctg gcgtacaaaa aatcattttt gcttgtgatg
    11101 caggaatggg atcaagtgcg atgggcgctt cgatcttgag aaaaaaagta caggaagcag
    11161 acttgcctca aacggtgacc aatcaagcaa tcagccaact gacagatgac tcgaatacat
    11221 tgatcgttac tcaggtcgaa ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt
    11281 ttgtctcagt agaaaacttt ttgaattcac ctcgatatga tgaaatcatt caagaattaa
    11341 gcagcaagca gaaaacatca gtttctgaaa ttgagaatga gccagcagaa gagtttcttt
    11401 cagcaaacat caaacaaatc aaagagattc tacttgtcca cgatgatcgt tccggatcag
    11461 caacaatggg gatgaccgtg ctcaaagata tattagacaa gaaccattta actatcccgt
    11521 taagaaaagt caatatccaa gatttaaaag atgaacctga ttcactgatc gtagccaaag
    11581 aaagtttagc ggaaaaagta cagatggctg cacctcaatc cattcattta tcagtcaaca
    11641 gcatggtcaa tccgcaaaaa tacgaatcga ttgcacaata tttgaaacag acagcttaag
    11701 ggaggaaaac agcatgcaaa gtttgccatt cgacatgatc gagctcaatc gatcttttaa
    11761 aaccaaagaa gaagcgatcc gctattgcgg tcagatgctt gtagaagcag ggtgcgttga
    11821 tgaggaatat atcgatgcca tggtagatag agacaaaatg ttatctgttt atatggggaa
    11881 ttttatcgcg attcctcatg gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat
    11941 ctgtgtcgtt caagtaccag atggtgtaga ttttggtacc gaagaagaag aaaagattgc
    12001 gactgttttg ttcggtattg caggagtcgg agatgagcat ttgcaacttg tacagcaaat
    12061 cgcactttat tgcagtgata tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga
    12121 agaagtaacg gaaaatctag caattgctta aggagagaga aaaatgagag cagtacattt
    12181 tggtgcagga aatatcggtc gaggatttat tggagaggtt ttggcagcta atggatttga
    12241 gattactttc gtagatgtga atcacacgat cattgatgct ttaaatgaaa gaaaagaata
    12301 cgaaatcgaa ttagcagatg aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat
    12361 caacaatcaa acaaatccag aaaaagtaat ccaagaaatc gctgaagcag atttagttac
    12421 taccgccatt ggcccgaata tcttaccttt tattgctgag ttgattgcaa aaggtatcca
    12481 agaaagagaa aaggaaggaa gcacaacatc tttagatatc attgcctgtg aaaatatgat
    12541 tggcggttca gcatttctag aaaaagaagt gtataaatat cttccggaaa catcatttac
    12601 tgacaaatat atcggctttc caaatgcagc agtcgatcga atcgtcccat tacaacacca
    12661 tgaagatcct ttattcgtcc aagtggaacc tttcaaagaa tgggtcattg acgatagcca
    12721 aagaaaaaat aaaaaaatcc aattaaaagg ggttttatat gtagatgatc tagaaccata
    12781 tatcgaacgc aaactattta gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc
    12841 cttgttaggt taccagacca ttgatgaagc aatgcaagat gcgttagttg tggcacaatt
    12901 aaaatcagtt ttgcaggaaa caggaagttt gctgattgcc aaatgggggt ttgatgcaga
    12961 gcagcatcat gcgtatattg aaaaaatcat tcatcgcttc caaaacaaaa atatctctga
    13021 tgcgatcaca cgtgtcgctc ggacaccatt acgtaagtta ggttatcaag aaagatttac
    13081 acgtccagtg agagaactac aagaacataa cttgacttgc ccacatttga cggcaacaat
    13141 ggggattatt tttaattact atgatcctga agatgaacaa agccgtcaat tgcatgaaat
    13201 gaagatccat gaaaacttgg aacaattgat tcaagaagtc acaggaatca atgatccgaa
    13261 aacaatcggg aatatcaagc aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag
    13321 gatacgaaaa aggggaatgt gacgaaattt ttgtcacatt cccctttttc gaataactag
    13381 gctgtttgtc cttcaagata gggcagatcc aattctttac cgtaacgttg ttctaaagct
    13441 aggcggatca aacgagctgc caggttctca tttcttgcaa ggataggtcc atggaaataa
    13501 gaaccgaaga catttcggta aatgacacct tcagattggt cttcaccatt atttccttga
    13561 ccttgtacca ctttccccaa aggtcgttca ccttcaccta agaaagtccg accattgtgg
    13621 ttttcaaatc cataataagt ttcattgaat tcttcgttat ggataacgat atcaccgata
    13681 aaccggttat tttcctgact taacgtgtag tgatccagtg cactgattcc ttggatcttt
    13741 tctccattag ctcccatgta gtagtgacct agcaattggt agccaccaca aatggctaac
    13801 atgacgccat cattttcgat atagttgatc aaagaatctt tctttttctg gatgtctttt
    13861 gaaatgatta attgctcaaa gtcttgacca ccgccaaaga aaaccaagtc atatttatca
    13921 gcatcaaaag gttcatgtat actaacgatc tccgtatggc aagaaacccc cattttttct
    13981 gctatatatt tcaacatcaa aaggtttccg ttatcaccat atgtgttcat caaatttcca
    14041 taaaggtggg cgatattgag ttcataatta gccacggttc attcctcctt tgataaatcc
    14101 ttgagcagct agttctttac gaagctgcaa tacagcagtg taagtagcta aaatatagac
    14161 atgctcagtt ggcaattctt tgattttttc gattacttct ggtaattctt ttgtttctgt
    14221 caatttattt tctggaatcc cggcaacttt tagacgtaaa gtcatatctg tatgacggtc
    14281 tcctccagaa ataacagctg ggatatccat atcggcaaag ttttcaaaat taccatccca
    14341 aatccagctg acatcgattc cgtctgcgta attggcattt aataaagcca ctaaagaaaa
    14401 tggataagga gctagaccaa tcatatcgat tacttgattg atacctacag gatttttgac
    14461 aagtactaat gtacattttt tcccattgat attgatgact tcttgtcggc cgaatacttt
    14521 ctcgtcatat cctaaacctt gtttgatttt atcaggatcg acattgaagt actctgccac
    14581 tgcagtagct gctaaagcat tgtagacatt atacataccg ccaacttcga ttccgtattc
    14641 atgaccatcg atcacgaaat ctgctgacac attgtccatc cgtaccatat cagttaatcg
    14701 ataatccaat tcaggacgtt tgaagtcaca atttggacag taatatttcc ctaagtttgc
    14761 ataagtgatc attttataat gcaagatgtg atgacaattt ggacataaga caccgtctgt
    14821 attgtaatga gccatttgtt cacgatcttc ttcatggtca aagccataat attttctagg
    14881 attgactgtt tcgatagaat tgaagattgg tgaatcaccg ttactgataa tcgttgcatt
    14941 tggtgacttt gcggcaccgt ctacaatcat tttataggtc gtatagattt ctccataccg
    15001 atccatttga tcacggaaaa tatttgtaaa taagaacagt tctggtttca tatattctgt
    15061 gactttgctc aaacttgctt catctatttc taggacagca aactttttct gtcctttttt
    15121 tggttttgcc tgcaagaaag tcgatacaat tccctgtacc atattcgcac cagtagggtt
    15181 ggttagaact tcatcgaatt gctggcgcaa aatattgact gttaaagcgg tagtcaatgt
    15241 tttaccgttt gttccggtga caacgacgac ttggtagtct tttgccaaac tatctagtat
    15301 atgtggatcg actttcagcg ctagtttacc tggtaaactt gatccgcctt taaaaaaagt
    15361 ttttaatgcc cattgcgacg tttttccaac tgcaatcgca aaatgactgc gtattcccat
    15421 tatgtaacct ccaaaaacga tttgctgacg aataaattca actaatcata ccatatgttg
    15481 aaaaaaaggt taaattgctt ttcttattct taagaaaagg ttacggctga aagtgcatga
    15541 aatcggacaa aatcgcatga tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa
    15601 gctgctgtga ttttattatt ccctcttacc tatattccca ttttaaccga taataatttt
    15661 ctcgcttgga tattcatagc ctagatctcg tgtttctttt ggcacgaata acatcaatgt
    15721 atatagcatt ccgatccgcc caataaacat cagtgtagca atcgtgattt tgcccacaac
    15781 agacaagtct ccggtaatcc ctaaagataa accagttgtt ccaaacgcag aagttacttc
    15841 gatgatgatc gagatcagcg tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa
    15901 acacatacca agagaaagca taaagacaac gactgatttt cgcacatcat cttgatcgat
    15961 tctccgtcca aaaatattga tgttatcttc acttttgagg aaggaatata gatacaaacc
    16021 gatgattgct acagtagttg ttcgaatccc gccgcctaca gaactaggac tgcatccgat
    16081 aaacatcagt aaagaaaaaa tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat
    16141 ctgcagacca gcattacgag tcgtaatcga ataaaacatc gaattgatcc atttgacact
    16201 catattggaa tcttggaata aatggtcctt ttccaataga taaatcaaga cagttcccga
    16261 aacaaacaag ataacaaaag ctaacacagc taatttggta aacaaggaaa aacgaaacgg
    16321 aagcttcgca ttggaacgtt tgtataatag ccattcccgg cactccatca atacaggaaa
    16381 accaattcct ccgacaaaaa tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc
    16441 aaagggcttg atggaatccc cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc
    16501 ctgataaaaa ccgtaaaaaa cggcatcacg ccaacgatca aaatagcctc tgtaataaaa
    16561 ataaatagaa aagaatgtcc caaaaagtaa ttgaaaccag attaaaatag caaaagtgat
    16621 ccgaatcagt cggacgatac cagacaaacg cggttgattc atatccgtca tgatcagctg
    16681 tcgctgtttc agtgtgatcc gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat
    16741 gatccccaaa ccaccaactt gaaataatat ttccagcagt ataataccgt tatcattgaa
    16801 cacagaattg atatcaaaag tcgatagtcc agtcacactc actgtactga ttgccatgaa
    16861 cagcatgtcc acaaaagaaa catggctatc tggttcgcga aaaatcggca tataaaatag
    16921 aacgagtgat aagaccgtca tcaatatata ataaaaaacg atgatttgga tcgatgagaa
    16981 atgattggca gcaaacttct gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg
    17041 cacagtacta tcctcctgac aatagtcgtt tctaccagta tacaagggga attaggaata
    17101 ctcaaagaaa ttatcaaaca gataacaata cagctttcga ttaaaataga aatggcttat
    17161 aataaggcaa atcctagaaa aaagaaggag gaggaataac aaattgatta atgctcaaca
    17221 aatcgtagaa aaaatgaaaa atcaaaaaat aaattatgat acagttctga aaaaaatgat
    17281 catgcaatgg gagaaagaag aagtaaagcc taagatatta atacatacat gctgtgctcc
    17341 ttgcagtacg cacacattgg aattcatgtc tcaatatgca gagatcacgt tatttttttc
    17401 aaattcgaat attcatccta aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt
    17461 tgtagaccaa tttaatgaga aaactgacca tcatgttgcg ctaatcgttg atgaataccg
    17521 tccaaatgaa ttcaccaaaa tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg
    17581 tgctcgctgc agtgcatgct tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga
    17641 actaggatac gattactttg gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt
    17701 gatcaatcag atcggaatgg atatccaaaa gatttacggg acacaatact tgccaagtga
    17761 tttcaagaaa aacaaaggct atgaacgctc tttgcaaatg tgcaaagagt atgatattta
    17821 tcgccaatgt tattgtggtt gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga
    17881 aatcaatcgg gaagcaaaag cttacttaaa aacatcaacc gtagtttttg aggaaaatta
    17941 aagaaatctt tcggaaaaac gtaagaaaaa aataaagaat ttatggtaga aataaggtag
    18001 actgtataat ttgtccgtag taaagtaagg aaacaagaat aaatttcaat gaaggaagag
    18061 ataagatgcg aaaagtagca cctattttga agccaatcgc tgtattaaca atctcgacga
    18121 ttgtcgcgac tactacaggt attgtgaata gttccagcta ccgtgcacca agttcaagtt
    18181 ctagtactgt agaagaaaca acaccaacta cttcagtctc gacatctgaa ccaacaacaa
    18241 cggaaagttt gaatgaaacc actacgacaa gtacatggga cgatacaaca actagttcgt
    18301 cgtatgatga gacatataca actgacagct caagcgaaac aagtaatacg gtaccagatg
    18361 gcactccatc ggattcaacc aatccaagta caagtccgga tacaagtgct agctccgaaa
    18421 cgataccaag cagtacgact gtagatgata acacgcaaaa tgatgatgca gcacgtacag
    18481 cacagcaaaa ccaagattca ggaaataccc aaacgtataa ccagcaagca ggtgattctc
    18541 aatgacattt ttactagacc ttatcaacgg ggtgcataaa ttccttggct atttagacat
    18601 cagtcctaaa tatcttaacc gtggatatac gattttaagt gtgattccca cgctttattt
    18661 attgagaatt gtttatggac tttggcaaaa ccaaaactat cttcaatttt tcttatatgg
    18721 acttgcattt ctagtgcttg tttattttac tgttttaaat gtattttatt actttttaga
    18781 taaaaacacg aaagccgatg tcactcagct ttttgtgaag tatttacctg atgaagcgtt
    18841 caacatccag acagaagaat cgatagataa tggaaattcg atcgataaag tgaatacaca
    18901 agaggtcatg gtttcttatg acgaagatta tcaattgaaa ttagccgaga atatgcggta
    18961 tttgattgga aatggtgaga tcaaaacaaa tgaactcggt cgcatggatg gatttctggt
    19021 cgatagaaat acattgtatc cttattacta tgtcaagaaa acaggagaga aagagtatgt
    19081 tctaaatatt ggacgaagct actcggacat ggaaaaagta gggaccattc gacttgactc
    19141 aacagaagac acattggaac ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga
    19201 cgggttacgc tatcatgagc cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca
    19261 ggaagaacca gtggtttata gtcgtagcca aagaagaaga cattcataat gtgatgaaac
    19321 gccgtgtatc agtggttgcg ataaaaacgt ttattcggaa taagggggat gtgacacgtc
    19381 ttttgtcacg ttcctttttt atataacgag aaagataaga cgaacacatc acttattctt
    19441 tcctttcatt tataaacatt gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg
    19501 taataaataa cgaggaaaat acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt
    19561 gggggatttt gatccttgta ttagcggcta ttttattagc tagagtgtac gtgtttacac
    19621 cagttactgt aaacgggcat tcgatggacc ctacattaag tgatggacaa cgattgattt
    19681 catctaagat ctcaaactat gaacgaatgg atattattac tacaaaggaa ccgggggatg
    19741 aagaacggat gattgttaaa cggatcatcg gtatgcctgg agatacagtc aagatggaaa
    19801 atgaccagtt gaccattaat ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa
    19861 aagaattttc agaagataag ttacaaggag aatatgctta tagctcagga ttccaagcac
    19921 aagcagaaag ttcatccacc tttacgagtg actttgaata tactgtgcca aaaggaaagt
    19981 atcttgttct aggcgataac cgcttgattt caaaagacag ccgaatgttt gggttagtcg
    20041 acaaagatat gatccaagga aaagttgttt tccgttattg gcctctttca gaaattaaaa
    20101 tcatgtgatg aaaaaataaa aagaagctgt gacatgtttt ttgtcacagc ttctttttcc
    20161 gaaaattcct tcttatttct tggagttgct cacttttttt gcaatctcat tttaagcttt
    20221 ttggctgatg gataagaaaa gcgaagcgtt tttgttcata aatagctttt ccccacgatg
    20281 gaaggtatga ctagtagcag agacaaagcc cgttttttcc aatcgattat tcaattcttc
    20341 ttggctaaag ccattatgga ctttcggatg actaatttgt tcattcttgt caaagtctac
    20401 gatgatcaat tgaccgccag gagctaaaat ttcataaagt tttgtaagaa tattctccgt
    20461 atcaggaatg tgtaaaagta caagtgatag aaggatgaga ttagcttgga cagcaggaaa
    20521 ttcgacagaa gcatcagcgt ggatcgttcg gacatttttc agatctgctg cttggatctt
    20581 ttcttctgcc attttcagca tagtttctga agcatcggca atgatcagtt ctttgaatct
    20641 ttccgcaagc ggtaaactaa caagcccagt tccgcctccg taatccagca ggactttttg
    20701 gtggctgtcg cgcggtagat aagtagttag ttcttcgcga ataatcttgg ctaatgcttg
    20761 acggtcaggg gaatcataat ggttagcgat ggaatcaaaa atattttttg tcatcaaata
    20821 gctccttacg taaaactttg aaattttaac ttattatagc acgtgaaaag aaagaaagca
    20881 tgctatactc aattcaaaaa gggagaatct agaaagaata tgccgatatc gtttgtaaaa
    20941 gatagagagg aaaaaggaaa gtgtgtccga gaaatattac tagacttgcc tgagtggttt
    21001 gggcttcctg aatcaacgga aaaatatatc gaggaatcaa gtaaactacc tttatggtgt
    21061 gaaaaaagaa aagaagaata tttaggcttt atcactcttt cacagacaag tgaagatact
    21121 gccgaaatct attctattgt atgggaataa aaaaagacta tcatcaccaa ggattaggaa
    21181 caaaactttt taaagaagca gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga
    21241 agactgtaga cgaggggcac tactctatat atgatcaaac tatttgtttc tacgaatcat
    21301 taggattttc aaggttggaa gtttttccaa atttatggga tgaatggaac ccatgtttag
    21361 ttcttgtcaa aaaattggaa caaaagtgag caatagattt aatactaatc tgtgcttgat
    21421 gaaaattctc actgtaaaaa atgctgaaaa tcaagtataa taccatagga acaaactgga
    21481 aggtgaaaaa gagcatggca caactatttt ttaagtatgg cgcaatgaat agcggcaaga
    21541 cgatcgagat cctaaaagtc gcacataatt atgaagaaca aaataagcca gttgtcttaa
    21601 tgacaagcgg tattgataca agagatggtt taggcgttgt ttcaagccga atcggactga
    21661 aacgtaatgc gatgccgata tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg
    21721 aaagcaaacc ctattgtgtg ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca
    21781 ttgcttttac gagaattgtg gacgaattgg atattccggt tatggcattt ggtttgaaaa
    21841 atgattttcg aaacgagtta tttgaaggat caaaatattt attgctttat gcagacaaaa
    21901 tcgaagaaat gaaaacaatc tgttggttct gccacaaaaa agcaatcatg aatttacatt
    21961 atattgatgg aaaccctgtt tatgaaggcg atcaagtgca aattggcgga aatgaagctt
    22021 attaccccgt ttgtcgaaaa cattactttc acccatcaat gattacagaa ggagaataag
    22081 catgtacgat caattacaag caattgaaga ccgttatgaa gagcttggag aacttttaag
    22141 tgacccagaa gtgatttctg atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa
    22201 cacgcgcgaa acagtcgaag tttatcgtcg ttataaagaa gtagtacaag gaatcaccga
    22261 tacagaagaa ctgcttggtg aaaaactgga tgatgaaatg gctgaactag caaaagaaga
    22321 attatctgaa ttaaaaaaag aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt
    22381 accaaaagat ccaaatgacg ataaaaatat catcatggaa atccgtggag cagcaggcgg
    22441 agacgaagca gcattattcg ctggcgatct tttcaatatg taccaaaagt atgctgaagc
    22501 acaaggctgg aaaacagaag tgatggaagc taacgttacc ggaatcggtg gatataaaga
    22561 agtgatcatg atgatcaccg gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc
    22621 gcaccgcgtc cagcgtgttc cttcaactga atcgcaaggg cgtatccata cttccaccgc
    22681 aacagttgtc gtgatgccag aagctgaaga agtagagatc gatattgaag acaaagatat
    22741 ccgtacagat atctatcatg cttcaggtgc tggcggacag cacgtcaata aaacagcatc
    22801 tgctgtacgt ttgactcact taccaacagg gatcgttgtt gctatgcaag atgaacgttc
    22861 tcaaatcaag aaccgtgaaa aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat
    22921 ccagcaagaa gcacaaagcg agtatgatgc caatcgaaaa tcagcagtcg gaactgggga
    22981 tcgttcagaa cggatccgga cgtataattt cccgcaaaat cgtgtgactg atcatcgtat
    23041 cggattaact atccaaaaat tagatcagat tttagcaggg aaattagatg agatcgttga
    23101 tgcattgatc atgtatgatc aaacatcaaa actggaagct atgcaaaatg ggtaaaatga
    23161 cctatcgaga agtcctgtca cgggcttctt cttttttaga ggatcatggg aaagaagcat
    23221 atagtatcca atttttattt ttggaacgaa aacaatggaa aaaacttgat tggctccttc
    23281 atatgaacga agagatttca gaagaagaac agcggttgat cgaaaccgat ctgcgacttc
    23341 tgcttgcaga tcatccacct cagtatttgc taggatatgc agatttttat gatcatcggt
    23401 taaaggtaac tgaagcaaca ctgatccctc gaccagaaac agaggagtta gtcgaatggt
    23461 gtttagacga gacgcctggt gtaccgttag aagtgataga tattgggaca gggactggag
    23521 caattgctat tagtttgaaa gcagcaagaa aaaattggca tatatctgct gtggatcttt
    23581 cagaagaagc attagaagta gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt
    23641 tttatcatgg agatacatta ggaccagtaa tggatcagca atttgatgtg attatttcta
    23701 atccgccgta catcagtcgg aatgaatggg aactgatgga tgaaagtgtc cggtcattcg
    23761 aacctaaaat ggcccttttt gctgagaacg atggattagc tatttatgaa aaaatagcag
    23821 ttgaagcacc aagcgtacta aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc
    23881 aaggtgaagc agtaaaaaaa atatttcagc aagcatttcc agataaaaaa gtggcgataa
    23941 aaaaggatct ttttggcaat gaacgaatga tccaagttta tggttgaagt tgtggatgaa
    24001 aaatttttta tttgtttttt tattataaaa aaacacttat ttgacaagga tgtaaacgac
    24061 tttttggttt atcaacaagt ttatccacta ttgacaaata gattatccac aaacagtgga
    24121 aaacttgagg ataaactcag acaaaataag aatgtatgaa aaaggggttg tggataaact
    24181 gtggaaacga aacgatatga tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa
    24241 ggagaacttg ttgcttttcc taccgaaaca gtatacggat tgggcgccaa cgctctttta
    24301 ccaaacgctg tcaagaaagt gttttctgta aaagggagac cgcaagataa tccattaatc
    24361 gtccatgtcg cctcttttga acaagtaaaa gagtatgtgg acaactttca tccggcagca
    24421 gagaagattg tgaagaactt ctggcctgga ccattaactt tgatatttaa gatcaaaaaa
    24481 gacactttgc cctcagttgt gacgggagga ttatccacag ctgcatttcg tataccggat
    24541 aacaaaaaaa cactagaagt aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca
    24601 aatacgtcgg gaaaaccaag tccaacgact gcagatcatg tatatcatga tctacaagga
    24661 aaaattaccg ggattattga tgatggagct acgcgaattg gtgtagaatc cactgttctt
    24721 gatttaagtg atccaacagc catgcctatg attttacgac caggtgcagt aacgaaagaa
    24781 caaatcgaag cagtcatcga atctcctgtt gctattgatc agcatcttgt aaaagaaaat
    24841 gaaacaccaa aagcccctgg gatgaaatac aaacactact cacccgatac acgtgttctg
    24901 atggtcagag atggcgattg gtcaactgca gttcaatggg caaaaaataa gaagatccgt
    24961 gcaggtgtca tagcaagccc cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt
    25021 tatatgtata acgacaactc agtagaagcg gcggctaaag gattgtttgc aggtctaaga
    25081 ggattagatg aaccaacact tggattagat ttgatttttg tacaagtgta tccagaaact
    25141 ggattgggga atgcttacat gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa
    25201 aaatgatcaa ttggttattg tcgcattctt ttagtatgat acaatgctat caagtaaatc
    25261 ttctatcagt gaacgaaaaa ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt
    25321 ggcaatttgt ttttgatggg aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt
    25381 gtggtagatt acaaaacgtt tgaccctgat ctttgggctg cgatcgccaa agaagaagaa
    25441 agacaagaac ataatttgga gctgattgcc tctgaaaact ttgtctccga agcagtgatg
    25501 gctgcacaag gaagtatctt gacaaataaa tacgcagaag gttatccagg gcatcgctat
    25561 tatggtgggt gtgaatttgt cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa
    25621 ttattcggag cgaaatttgc gaacgtccag cctcactccg gctctcaagc caacacggct
    25681 gcttatctcg ctttggtaga accaggggac acgatcttag gaatggatct ttccgcaggc
    25741 ggacatctaa cacatggatc accagtcaac ttcagtggca aaacctatca ctttgttgct
    25801 tacggtgttg atccgacaac agaagttatt gattataatg tggtacgtat tttagctaga
    25861 aaacatcaac caaaattgat cgtagcaggt gccagtgctt acggacgaac gatcgacttt
    25921 gcgaaattca gagaaatcgc agatgaagta ggcgcaaaac tgatggtaga tatggcacat
    25981 atcgcaggat tagtagcagc aggacttcat cctaatccag ttccttatgc agatatcaca
    26041 acgactacca ctcataaaac attgcgtggc cctcgaggcg gcatgatttt gacaaacgat
    26101 gaagcattag caaaaaaaat aaatagtgcg gtatttcctg gtattcaagg cggccctcta
    26161 gagcatgtga ttgcgggtaa agcagtagca ttcaaagaag cattagatcc tgcatttaaa
    26221 gaatatagtg aacaaatcat cgccaatgca aaagcaatgg tcaaagtttt caatcaagca
    26281 attggaacca gagtcatttc aggggcgaca gataatcatc tgatgttgat cgatgttcgc
    26341 gagttaggta taaatggaaa agaagcagag agcattttgg acagtgtgaa tatcactgta
    26401 aataaaaact cgattccttt tgaaacacta agtccattca aaacaagcgg tattcgaatc
    26461 ggcacgccag caatcactac tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa
    26521 ctagttgtca aagcattaca agcaaaagat gataacgctc aactagacga agtcaaaaca
    26581 ggtgtacgcg aattaactga aaaatttccg ttgcataaaa aataaaaatt taaattccat
    26641 tagtaaacag aaaagttcaa tgttgggctt ttctgtttat tttttgggaa aacggattct
    26701 ttgacaatat cggtgatgct ctctagtata tgaggtgtaa ttttagtctt cacactaaat
    26761 ctcagggagg aaaagtcatg actgatcgta tctcgttaga aaaggaagca gtagcattca
    26821 gtgatgcaaa tgcgcctcat ccgcgtatct atgaactagc accggaagaa ggaagagttt
    26881 tactagaaga agtacaagaa tcacctgtga acaaatattc cgtcgatatt gaagacttga
    26941 tggtggatac tggcgaatgg ggaaaactca atattcgtct cgtacgacca gaagggaata
    27001 tggataaatt accagttatt ttttatattc atggagcagg ctgggttttt ggcagtgctc
    27061 aaacccatga taaattgatc agagaacttg cggttcgtac gaattccgtc gttgtctttc
    27121 cagaatatag tcgatcacca gaagcaaaat atccaacagc aattgaacaa aattatgctg
    27181 tgttgcaaca gctgaataaa ttagccaagt caaaaaatct ggatctcgag aatctaatcg
    27241 ttgcaggaga ttcagtaggt ggtaacatgg ctacagtcat gacgatctta acaaaagaac
    27301 gaaagggcct cccaatatcc aagcaattgc tgtattatcc agtcacaaac gctgattttg
    27361 atacagattc ctatcatgaa ttcggagaaa actatttttt gacaagagaa ggaatgatgt
    27421 ggttttggga ccaatatacg acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt
    27481 tgagagcaaa tataaatgaa ctaaatgatc ttccttcagc aatgatttta acgggagaag
    27541 cggatgtatt aagagatgaa ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc
    27601 ctgttacaca agtacgcttc caaggaatca tccatgattt tgtcatggta aatgctatgg
    27661 atcagacaaa ggcaacgcgt gcagcaatgg cattatctac ttcttggatc aagaaaaaag
    27721 aataagcttt gcttgtccgc aggaaaactt cttttcaaaa acggattttc ttaggaaaaa
    27781 tggcgagttt gactagtcaa tcacttgatt ttgttttaca attaaaaaga aacgacagaa
    27841 caaaggagag cttaaaaaat gggcaaattt caagtgatcg atcatccatt gattcaacac
    27901 aaactaacta ttattcggga caaaaactgc ggaacaaagg tttttcgtga agtagtcgat
    27961 gaaatcgcta tgttgatggc atatgaagta tcacgggata tgccattgga agacatcgtg
    28021 atcgaaacac cgatcactga aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt
    28081 attccaatct tgcgcgcagg tattggaatg gttgacggta tcttggaatt gattccagct
    28141 gctaaagtgg gacatatcgg tctttatcga gatgaagaaa cattggaacc acatgaatat
    28201 ttcgtcaaac ttccagaaga cattgatgca cgtcagttgt ttgtagtaga tccaatgttg
    28261 gcaacaggcg gatcagcaat catggcaatc gatgcattga aagaacgtgg cgcgtcgaac
    28321 attaaatttg tttgtttggt agcagcacct gagggagtaa aagctttgca agaagcgcat
    28381 cctgatattg acatctatac agcttctcta gatgaaaaat tgaatgaaca tggttatatc
    28441 gttcctggac taggggatgc gggtgaccgt ttgtttggta caaaataagt aaatacgaaa
    28501 aagggtgtga cacaactttt gtcacatcct tttttggtca tttttaacgt ttgatgcaga
    28561 acagcttttt cacagctttc gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg
    28621 tcaaattcaa atctttttta gttctttgta gttaacgatt actacaccct tactttgcgt
    28681 catcaagttc gcattttctg cagtttcatc aatctcaaca acagcggaat ttttcaattg
    28741 ctttgaaatc gttccagttt gcgacttgcc gtgaattgtg aacattacgt gagtattgat
    28801 cttgaatttt tcaccaattt ccgatggtac gatagttgta tcaaattttc caaaagttgc
    28861 cattttcata aaacctgcct ttcacatcaa ttataccacg ttttatgaaa aatatcatga
    28921 aattaaacta attttccatc ggactcttta caatcagcca aatagagtat ataatttctt
    28981 ttggataagg ccagcttagc tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta
    29041 ggttcgatcc ctatagctgg cattaaaaga gaagacagat atatcaagga tttttaatcc
    29101 attgatacat ctgtcttttt gtttaaagga aataaagtat gctttcttag caatagagca
    29161 tgtattattt ctataaaaat ttaggagaat aaaacgacta gcaatatagc tgtgaaatca
    29221 aatatcacat gaactgccca acttgtaatg atcgagttgc ttttaatggc agcttggttg
    29281 aataacaatc ttgccacccc ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt
    29341 ccattatcat aagtactgta atggataaat ccaaagatga ttgctgacaa gaaactagcg
    29401 acaggaatgc tgaacttact agaaaaaata gcgagaaaat acactgagaa caattcttct
    29461 ccaagtatca tgataggcaa aataataaat aataacgggc tgaaagcctc atttattgga
    29521 ttgcctgtta aaccccattt caaaatttgt tgcaaaaata ccgagactgc ggcactgatt
    29581 actacattga taaaaaaata tttggctata tttttgacag gagcgacagg tttcgagaac
    29641 atttttttcg tatctttttt accaaaaaca agtgtaaatg ctataaatcc agtgatagtt
    29701 aaagaaaaaa tgctaataat ggatggcgca aatagcaacc ctaacaaacc gagtacaacg
    29761 acaatagtgc ccaatatccc ttttttaagt tctatttttt ttcctaaaat gttattcatg
    29821 ataataactc ctactattca gtttttgaag atagagtcag tatacatgaa gagttaaaat
    29881 aggcaatgtt tttttatgga tatttgtatt gctaaaaatt tatcttatat attcattttg
    29941 attatactac ttttatttgt aaaaattttc tagcaaaacc aagtgcttgc aactgattaa
    30001 gcgtacaatt ttttagatgt tccgagcgta cttcaatcat ttcaaaggag ttgctactta
    30061 aatcgatatt ttttaaatca gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa
    30121 acattatttt agtgagggaa cagtctacaa aatctgcgct tgataagaca gagtgctcaa
    30181 aggtactttc ttttatgttt gaaccagata aatttacata gtccatttta caattctcga
    30241 ataacgtgtt cccaatataa ctttccatca atttagtacc tgttaattta caattgacaa
    30301 atttcacgcg atgcaacatg ctttttgtca atatacagcc tgaaaaatca cagttttcga
    30361 atatacagtc ggtaatctcc attctttgaa aatcattgga agcaaaaaat gcatacttga
    30421 aaatagattt gtaaataatt ggatggcaga ttatttggtt ttcgatgacc tctttttgtg
    30481 aaaggatatt atacagttca aaatcttcgt tttcatagat atgaaaaaag gatcctttat
    30541 tcaagcttgc ttcattaact ttaggaaaaa tcatatttaa ttacctccat tttcataaag
    30601 tcatattaac aaaagctaaa ccaacttaca ttagcagaaa atgagatagg ttttcgcctt
    30661 tttcgataac gatagtgtcg ctatctcttg aaacgttctt agaattgccc tgagagacac
    30721 aaattaggcg taattctaat aaaaatctta gaataaaaat ttttactgat ttaatttaga
    30781 atgtttttaa aataaaaatc gttgccataa cagtgtattg aaaggtttta acaatccagt
    30841 aatgatttaa aaaaatctag atgtagcgta tactttgttt atagaatgta gtgggaagaa
    30901 atatattttt gggaggaaat cgtgatgtct aaaagtgaag aaatagaaaa catggtacta
    30961 aaattatatg atctcgtttt aaatccagag atcaaagaaa aggaacgtat acttttgata
    31021 gacgcaaaga caggcttaga aaaaggtcag tattatccta aagtcataaa taatttggaa
    31081 cgtagtttac gtccgttagc aatcagggga gaattgtcta aaccagtatc accgttttat
    31141 atggagatat caacaatagg aaaatttgaa aaagaattag gtcgaggaat ggcttcggca
    31201 ccgattactt ttgggcattt ataaaaacga gttattcatc tcaatgaatt aagaagtata
    31261 gtcaatctca taatgcaggc taggttcatt tatttgaaca gagtctgtat ttttttattc
    31321 taaataaaga aacagatgtt tctttatatt gtattttatc ctttaattaa cacaaaaaca
    31381 gctagtataa actaataatg ttaattaaaa gtgtatttta tgaaactatc gttatttatt
    31441 aatgttgcat ttttgtaagt taaaaattgg aaatatagat tgaaaaaatg aaagcaataa
    31501 gtaaaataaa atgaatatat gcgaaaaaat aatttacatt aggaaaaacc ttgtaaaaac
    31561 agttttttgg catatttatt ttttataaat atttgtaaaa taactatcaa ttttttagtt
    31621 aaaagattgg aaatgagtgg gggaggaatt gaagaaagga tttttgagag atgatatagt
    31681 aggttttatt tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt agaaatatta
    31741 ttttatcaat gttatttatt attactaata taacatcact gattcctgtt catgtctatg
    31801 cggatgcagg cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata
    31861 ttacagatgg aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc
    31921 aaccaggtga ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat
    31981 tccaaaaaga aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa
    32041 aagattctgc gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt
    32101 gggggcaatt tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac
    32161 cattcaccgt gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg
    32221 gttcttcttc tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc
    32281 acattcgatg gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa
    32341 tcagtgatga aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa
    32401 tacacttagg tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag
    32461 atttccccgg cgctacattt aatttttcag taactgataa ttacattgaa ataactatac
    32521 caaaaaactt tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc
    32581 cagaacaaat aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg
    32641 ctgttgatgg agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca
    32701 acggtacagt tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta
    32761 tcccaaatgt gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta
    32821 gtatcctgtt gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag
    32881 actatgttgt aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa
    32941 atgaactcaa attctccata aacgaaaatg atacagaggg agtcagttta cctatttata
    33001 acaagaaaaa ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa
    33061 gaccgagtat ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg
    33121 atgcagaaac aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac
    33181 agtatgatga tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa
    33241 atgattatgt cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga
    33301 ataaagaagt catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg
    33361 gcaaacgacc gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca
    33421 aagaagtaag tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag
    33481 acgggcaaga aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga
    33541 ttgacgggta taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag
    33601 gtagccatat aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa
    33661 atctattctc tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa
    33721 gcaattatat tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc
    33781 ttcctaaaac aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa
    33841 ctatctgtgc tgggatattc ttacaaaaat ataaaaaagt ttagttagaa tataatattt
    33901 tttgttaaat atgactgaaa tcaaaagaat aaaattccga tttataatcg aaaaaagaag
    33961 catttttata tatgcttctt ttttcgtaga atcaataaat atggatttgg ctaactatgg
    34021 aagaatactc gtattagttt tcagcgttca tactagttgt tacattgttt gattggccaa
    34081 aagaatttct gatatatttt gtgctaaata aagaaaaaga cgcttgaatt gggaaataag
    34141 aaaactactc tatcattaca aattttcata aactggcaga tgttctaagt aaacaagtat
    34201 cttttcaaag tcggaaaaat ccataatcaa cgtagtggta ttgatatttg gatgaaagcc
    34261 tacggtatct tgacgatcaa tagcagcatc gatcacgact tggatttttt tctctttgtc
    34321 gtgcatcaat gctaatggag tcactgtacc tgctggaacg cctaggaggt caggaagctg
    34381 ttcttcagaa acaaaagata agcgcttttc ttttaattgt tcagctagtt gttttaaatc
    34441 ggcgtgcttt tcatcaggca agatgacaag atagatttgt tttccttttc ttgatttcaa
    34501 taatagattt tttacttgcg gaccaggtag ggaaaaaggg acatttttga ctgacgtaat
    34561 cgcaggatga tcgacttttt gaaaagagat acccagtttt tttagtagtt cataagcttc
    34621 ttgttcggtt gcacgactca tcgcgcatca ccctcctttt tttattagtg tatcataaaa
    34681 aagagttgac aaatgaaaaa taatcatgta aattacacgt aaagttaaaa caaatgacat
    34741 cgaaaagaag agtagccgtt aggtgttttt agagagtctc tggttggtgg aaagaggcag
    34801 cagttaaccg tgaaacacat cttggagtcg attttctgaa gcgtatagta ggaaaatccg
    34861 gggagcccgt tacagctgcg gtcgttgcta gatgaccaga taaggctggc ttttccgtga
    34921 ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg
    34981 actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat
    35041 aaacagaggt ggaaccacgt ataatacgtc ctcttggcat ttgccaagag gattttttta
    35101 ttttaaagga ggaaaagaag atggagtatt tgattgaagt tatgccgcaa ttattagtag
    35161 gagcactcac aacattgaaa ttatttttat ttaccttgat tggctcatta ccattaggga
    35221 ttgttttagc attaggattg tctttgcatt ttaagccgct acgctacttg cttcaaacat
    35281 atgtatgggt tatgcgggga acaccgctgt tgttgcaact gatttttatt ttttatgggt
    35341 tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc tttatcttaa
    35401 actatgcagc ttattttgca gagatctttc gtggagggat acaatcgatt cctcaaggtc
    35461 aatatgaagc agcgcaagtt ttgcaattaa gtccctttca aacggtgacg aaaatcgtca
    35521 tcccacaagt gacaaagatt gttttacctt ctgtagggaa tgaagttatc aacttggtca
    35581 aagattcttc cttgatctat atattaggtt taggtgacgt aatgcgtgct gggaaaatcg
    35641 cgatggaacg cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc tatcttacat
    35701 tgactggtat tctaacaatc gtaatgaaac aagttgaagg aaaattaaat tattaccgct
    35761 aactgatagg aggaagaaat atgattgaat tgaagaaagt gaacaaagca tttggtgaaa
    35821 aaaaaataat caaagatttg aatctaatca ttccagatgg gaaaacatta gctatcgtag
    35881 gtccttccgg tggcggtaaa accacgcttt tacgtatctt ggctggctta gaaagttctg
    35941 acagcggaga attttattta gacggtgtac cgttcaaccc attcgagctt aaaatgaaag
    36001 aacaagttgt aggagtcgta ttccaagact tccagctatt tccacatctg aatatttttg
    36061 aaaacattac cttagcacca aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa
    36121 aagcagaaga attagcggct tcattaggga ttagtgattt attgaatatc tatccttatc
    36181 agctttctgg cggacaaaaa cagcgggtag cgattgccag agcattggct atgaatccac
    36241 gggtattggc ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg
    36301 agcgtttgat cctatcattg aaagaagatg gggtaaccca aattattgtc acccatgatt
    36361 tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt cattaagaaa
    36421 ggagaagata gagatgaaaa aaaccagttt tctattcgct ttgatcgcag gtcttcttct
    36481 ttttgcaggc tgttcgaaca aaaaaacttc agcagataat tgggcgacta tcaacgaaga
    36541 gaaaaaaatc gttataggtt tagacgatac atttgttcct atgggattta gagataaaga
    36601 cgggaagctc acaggatttg atattgattt agccaaagca gtctttgaac aatatgggat
    36661 tacggtagac tttcagccca ttgactggtc aatgaaggaa tttgaactga acaatggaac
    36721 gatcgatttg atatggaacg gctattccaa aacgccggca agagaaaaga aagtgcaatt
    36781 tacaaaacct tatatggaaa acgatcaagt gctgatcaca cctaaagaat caggaatcac
    36841 aaactttgga caaatgaata ataagagatt aggtgcgcaa aatgggtcat ctggttatga
    36901 tgtatttacg aaacaaccag aagtattaaa agagatagtc aaaaaccaag atgctgtttt
    36961 gtatgatagt tttaatgaag cattgatcga tctgaaaagc agacgggtcg atggtctgct
    37021 gatggataaa gtatacgcag actattattt gaaacaaaga aacgaactag aagacttcaa
    37081 cataacaaaa ggttcttatg agagtgagga ttttgcagtt ggtgcacgca agtcagatag
    37141 tacattagtg aaaaaaatca atgaagccct agatcaacta gagaaaacag gtgaattcca
    37201 aaaaatatcc gataaatggt ttggcgaaga tgttacgccg agaaattaac aaatagggga
    37261 ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa acagtgttct cctgccaact
    37321 ccttgtgact cggagctgaa tggcaggctc acaacctctt ggtaaacggt gttcaaaagc
    37381 cagtttttcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt
    37441 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
    37501 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
    37561 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
    37621 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
    37681 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
    37741 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
    37801 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
    37861 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
    37921 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
    37981 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
    38041 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
    38101 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
    38161 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
    38221 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
    38281 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
    38341 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
    38401 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
    38461 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgcaggaaa
    38521 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
    38581 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
    38641 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
    38701 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
    38761 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
    38821 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
    38881 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
    38941 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
    39001 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
    39061 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
    39121 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
    39181 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
    39241 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
    39301 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
    39361 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
    39421 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
    39481 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
    39541 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
    39601 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
    39661 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
    39721 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
    39781 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
    39841 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
    39901 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
    39961 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
    40021 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
    40081 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
    40141 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
    40201 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
    40261 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
    40321 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
    40381 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
    40441 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
    40501 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
    40561 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
    40621 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
    40681 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
    40741 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
    40801 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
    40861 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
    40921 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
    40981 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
    41041 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
    41101 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
    41161 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
    41221 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
    41281 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
    41341 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
    41401 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
    41461 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
    41521 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
    41581 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
    41641 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
    41701 ccaaaagaaa aaatggtttt tttccatctt
//
LOCUS       DACEDM010000014        41710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEDM010000014 DACEDM010000000
VERSION     DACEDM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.014"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(19..729)
                     /locus_tag="IWO50_000722"
     CDS             complement(19..729)
                     /locus_tag="IWO50_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0357863.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="IWO50_000723"
     CDS             complement(726..2198)
                     /locus_tag="IWO50_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0357864.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="IWO50_000724"
     CDS             2336..2809
                     /locus_tag="IWO50_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0357865.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="IWO50_000725"
     CDS             complement(2962..3513)
                     /locus_tag="IWO50_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0357866.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="IWO50_000726"
     CDS             3618..4148
                     /locus_tag="IWO50_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0357867.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="IWO50_000727"
     CDS             complement(4631..5074)
                     /locus_tag="IWO50_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0357868.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="IWO50_000728"
     CDS             complement(5090..6250)
                     /locus_tag="IWO50_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0357869.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="IWO50_000729"
     CDS             6757..8457
                     /locus_tag="IWO50_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357870.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="IWO50_000730"
     CDS             complement(8559..9392)
                     /locus_tag="IWO50_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0357871.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="IWO50_000731"
     CDS             complement(9696..11861)
                     /locus_tag="IWO50_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0357872.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="IWO50_000732"
     CDS             12121..14415
                     /locus_tag="IWO50_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0357873.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="IWO50_000733"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="IWO50_000733"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0357874.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="IWO50_000734"
     CDS             15066..16073
                     /locus_tag="IWO50_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0357875.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="IWO50_000735"
     CDS             16095..17114
                     /locus_tag="IWO50_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0357876.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWO50_000736"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWO50_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0357877.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="IWO50_000737"
     CDS             complement(18871..20292)
                     /locus_tag="IWO50_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0357878.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IWO50_000738"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IWO50_000738"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0357879.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /locus_tag="IWO50_000739"
     CDS             complement(22703..23959)
                     /locus_tag="IWO50_000739"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0357880.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            24539..25840
                     /locus_tag="IWO50_000740"
     CDS             24539..25840
                     /locus_tag="IWO50_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0357881.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="IWO50_000741"
     CDS             complement(25909..26508)
                     /locus_tag="IWO50_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0357882.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26762..27643
                     /locus_tag="IWO50_000742"
     CDS             26762..27643
                     /locus_tag="IWO50_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357883.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27656..29266
                     /locus_tag="IWO50_000743"
     CDS             27656..29266
                     /locus_tag="IWO50_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0357884.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29343..30002)
                     /locus_tag="IWO50_000744"
     CDS             complement(29343..30002)
                     /locus_tag="IWO50_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0357885.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30285..30950)
                     /locus_tag="IWO50_000745"
     CDS             complement(30285..30950)
                     /locus_tag="IWO50_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357886.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31097..31702)
                     /gene="dhaL"
                     /locus_tag="IWO50_000746"
     CDS             complement(31097..31702)
                     /gene="dhaL"
                     /locus_tag="IWO50_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0357887.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31730..32713)
                     /gene="dhaK"
                     /locus_tag="IWO50_000747"
     CDS             complement(31730..32713)
                     /gene="dhaK"
                     /locus_tag="IWO50_000747"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0357888.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(32715..33110)
                     /gene="dhaM"
                     /locus_tag="IWO50_000748"
     CDS             complement(32715..33110)
                     /gene="dhaM"
                     /locus_tag="IWO50_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0357889.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33264..33584
                     /locus_tag="IWO50_000749"
     CDS             33264..33584
                     /locus_tag="IWO50_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0357890.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33696..34565)
                     /locus_tag="IWO50_000750"
     CDS             complement(33696..34565)
                     /locus_tag="IWO50_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0357891.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(34661..35965)
                     /locus_tag="IWO50_000751"
     CDS             complement(34661..35965)
                     /locus_tag="IWO50_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0357892.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(35962..36471)
                     /locus_tag="IWO50_000752"
     CDS             complement(35962..36471)
                     /locus_tag="IWO50_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357893.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36646..38781)
                     /locus_tag="IWO50_000753"
     CDS             complement(36646..38781)
                     /locus_tag="IWO50_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0357894.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38864..40519)
                     /locus_tag="IWO50_000754"
     CDS             complement(38864..40519)
                     /locus_tag="IWO50_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0357895.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40521..40835)
                     /locus_tag="IWO50_000755"
     CDS             complement(40521..40835)
                     /locus_tag="IWO50_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0357896.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41005..41265
                     /locus_tag="IWO50_000756"
     CDS             41005..41265
                     /locus_tag="IWO50_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0357897.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41279..41593
                     /locus_tag="IWO50_000757"
     CDS             41279..41593
                     /locus_tag="IWO50_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0357898.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT        12222 a         8598 c         7518 g        13372 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 taaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatgtata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    26701 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    26761 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    26821 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    26881 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    26941 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    27001 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    27061 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    27121 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    27181 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    27241 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    27301 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    27361 cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt
    27421 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    27481 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    27541 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    27601 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    27661 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    27721 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    27781 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    27841 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    27901 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    27961 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    28021 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    28081 gcttttagca atatcgatca gctgtacgat gatttttttc caagcggatt cgatcactgc
    28141 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    28201 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    28261 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    28321 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    28381 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    28441 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    28501 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    28561 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    28621 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    28681 tgaaaaaaac tttacaagca cgatcatggt agtcttgatt tgtctagctg ctattttacc
    28741 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    28801 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    28861 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    28921 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    28981 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg
    29041 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    29101 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    29161 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    29221 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    29281 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    29341 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    29401 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    29461 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    29521 ttagctaagt caagcagata gacagcgact cgttcttcta ctttttccat catcaaaaaa
    29581 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    29641 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    29701 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    29761 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    29821 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    29881 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    29941 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    30001 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    30061 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    30121 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    30181 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    30241 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    30301 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    30361 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    30421 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    30481 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    30541 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    30601 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    30661 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    30721 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    30781 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    30841 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    30901 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    30961 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    31021 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    31081 cgcagtttta ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg
    31141 cactagaagc agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc
    31201 gtcctttttt agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg
    31261 ttaaattccc tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat
    31321 cgtcagcttc tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc
    31381 ctaatttttc ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga
    31441 atgcagatcc gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga
    31501 atactttgaa tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag
    31561 ctgaaacccc tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat
    31621 cactgagata ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga
    31681 tttcttgaac ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa
    31741 tcgtctgtac attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg
    31801 ttagagaaag accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta
    31861 cattgacgcc tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca
    31921 tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt
    31981 aatcatctaa aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt
    32041 ctcgacgata gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta
    32101 gttcgaatcc tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg
    32161 tcttagttgc ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt
    32221 ttcttgctgc atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc
    32281 cagcagtata cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt
    32341 caatatcgtc catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat
    32401 aattcttaac gatcagcaaa actccttttc cactatcggc ttccttaata gccgtctgaa
    32461 tctgatctgg agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt
    32521 cccctacaaa cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa
    32581 cttgttccgc tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt
    32641 ctggatagct ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca
    32701 tgattttttt caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt
    32761 tgctgagtat catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa
    32821 ggcgcatcca ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt
    32881 tccgagttca ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt
    32941 gattcattga ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg
    33001 ccgagagaaa aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata
    33061 ccatctgtta ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc
    33121 ctttctttta catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat
    33181 ataataaatt tctaattttg ggaaaatatg tgtagtgctt cttgaagttt gttatacttt
    33241 aattaaaaga gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag
    33301 ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta
    33361 aagacttgga ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg
    33421 aactaatggg tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag
    33481 gagctgatgc gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca
    33541 tgctgatggt cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt
    33601 ttaataacta aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta
    33661 acttcgaggt actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg
    33721 taagctgctg cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt
    33781 tcatttgttt ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact
    33841 gtttctgtcg tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt
    33901 attcgttgga aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt
    33961 tgtgcttcgc tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt
    34021 tgacttagct ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt
    34081 gcttgttgga tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg
    34141 gcaagctttt tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa
    34201 tcttgatcga ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct
    34261 tcgagttctt ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta
    34321 gaactattta ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt
    34381 acattggaag tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatggtt
    34441 gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag
    34501 aaaatactgg aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt
    34561 cccatcctta ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca
    34621 taaacacaac aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg
    34681 ggcttttcca tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg
    34741 tcacgatcgt tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa
    34801 ggtatttcaa ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc
    34861 ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt
    34921 cgtagtttcc ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac
    34981 aaaagaatat tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa
    35041 tatgtttaaa aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac
    35101 cttttgccca acgttttctg tgtcgatata gcatcttcaa cgtcacaggt acttccatga
    35161 aaaagataat ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa
    35221 tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt
    35281 ttcgatacat cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg
    35341 ttcgtttaat gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt
    35401 tggtacgatt ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc
    35461 gaatgaaata attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat
    35521 tgcttaaaat caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc
    35581 ctttattttt ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta
    35641 atatttctgg tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat
    35701 aattcaattt cgtcatcaaa tactcgattg tatattcgat cacaatttct tcattatggg
    35761 ctggcaccat gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt
    35821 gcttatgttt gtataaaaac acataacacc agacaccaat aaaccataca aatccgccta
    35881 aaatcggata agaaacgaga aaaaaataca taatagacaa acaagtatct aagaataaat
    35941 tacctgtatg ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc
    36001 aatgaatgtc gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct
    36061 ctcttctttt gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt
    36121 caatttttca tcttcatatt caaaagattt atttactttt ttgtatagat ttcgattgtt
    36181 ccacctagtc aatacaaatg aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc
    36241 gattaagaaa tacagcattt tcttttctga taaaaaagcg tacaaggaaa aatccttggc
    36301 tctttctggt ctcgttaatg ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca
    36361 tcctaaaaga gccactaacg tttgccaaat ctttaaccag taattacctt ttgaaaaata
    36421 cttatctttc accaaaattg atgcaacttc ttctcttgtt acttttttca tatcctcacc
    36481 tcacgttttt ttgttacgac gtaggagttt accatacatt tcatttcttt tcacataaag
    36541 tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata
    36601 ttttgttctt tttatcagtt gttcttttta ttttttatct cttaattatg ttgctctttc
    36661 caatacgaca atgcttttca ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct
    36721 ttgctcatct ccatcgtttt caaatggata taagattaca tgatcaaatt ccagtccttt
    36781 tgctagactc aaaggaatga tttggcagtt ttctaatcca tatgaatttt ttttaatcga
    36841 ttcttgtagc gtttgggttt cctcttctgt ttgggtgata actgccgctt tttctccttt
    36901 tagagaaacc aaaatctcag ctagtatttt caacaattct tcagaggatc gaaaagagcg
    36961 gtattcgggt tcttttcctt gcggtcggat cgatacgatg tcgatcttct cttgatccac
    37021 agcataggtt ttgaaaagct ctgtgattgc tccgctagaa cggtaactat ttctaagatc
    37081 ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt
    37141 ggaagaatta aagattgcct gattctcgtc acctactaaa gtaaagtgag tttttggaaa
    37201 taattcgatc aatagaccta attgtgccag ggtataatct tgtacttcat caatcaatag
    37261 atagcgaaat gctggtacat ttaccttttc aacaagtaaa tgacgaatag ctaatataat
    37321 gactgcttca tcaacagtac gtgtgggttg ccaagcataa gctgagccat atcgtttttc
    37381 ataaattctt tcaaacaact gatgctcatc cacccatgcc atttcttcga tttgtctggt
    37441 tatttttcga tatttttttt gtaaaagttt ccttgcataa gctacaatac tttgttccga
    37501 atcatcagaa atcaatttac cgaataattc caactgccta tcttctgtca acgagagtac
    37561 ctgattctgc acagcaggac tttttgcttg tttcaaaaga tgattcttcc attcgctcat
    37621 cagtgccttt ttcgttgctt gaagcctatc gatcaattta ggatgagcag gaactttctc
    37681 atatattttt tcgatcgttt ttttagaaag gactgttttt ccatttttag taagtccacg
    37741 gataaaattc ggatgatcta aaaacaaagg atctgattgt tttagattat cgataaactt
    37801 tttgctacgc aaacgttctg tctgctccga tacattttct cccgtgatac gtttgaaata
    37861 agcctcttca ccttctattt cctctgctga aagctgtgaa accagctgta tgatggttag
    37921 attcagtgga tttctttcac ctaatgatgg taggacattc gaaatatagt cgataaaccg
    37981 atgattaggt gatagaatca gcaagtcatc ggatgtcatc gtatctcgat ataaatagag
    38041 cagatacgca atacgctgca tgatagcaga ggttttacca cttcctgcta cgccatttac
    38101 caagatattt tgacttttca catcccgaat gattgtgttt tgttccgatt ggatcgtagc
    38161 agtgatagcc ttcatttcat tggtctcatc ttggcctaag acttctaata atacgtcatc
    38221 ttgaatagct acagacgtat caaaaaactt gaccaaacga tctctttcca aaagaaattg
    38281 tcgtctttct ttaataaccg tatcgatttt attttgacga accgtataag aagatggacc
    38341 aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa caagattttc
    38401 atctgcatca tctgtgaaat ttgctgtacc gatataaaag gattcttttg gctcattatc
    38461 cagaaagtct acaacgactt ttccaaagta gggacttttc aataaacgtt cgatctttgc
    38521 tagctgattt tctgcgtttt tctgtttgat attcatctga tcgatttctc tatttttcat
    38581 ttcaatcatc gaaaaagtat ccaggttatc taagtaactg tcaaaattca atcgaagctc
    38641 gccagccatt tctttcaacg actggtttcc ttctattttt gccgtatcca tcgattcccg
    38701 cagttcgttt tttttatcaa ttagcttttg atacacgttc ttcagatatg cttcttctaa
    38761 ctgtttttgt gtgttgacca tacacattct cctctttttt taaagtggtg gaaccgatta
    38821 gtttacctct tttaagagtc gaatgcaagc tttttaacga gtatcatttg ttaggatata
    38881 tcagttcttc aggtcggaca gtttgatata aagaaaatgc accatcaagg ttttttacgt
    38941 ggtagccaga ctgcttcaag attctttccg ctagatagct acgtaatccg ctatgacagc
    39001 tgacgatata ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt tcattcaaag
    39061 ggatatgttt ggcttccttg aatttgccct tttccaattc ttctgctgtt cgcacatcta
    39121 ataaaatttt tccttttgct agttctgtcg gcaattcatg ccattgtacg gtttcactaa
    39181 gcccttctgc aatgttcatt gctgcatatc ctagcatgtt tactggatct ttggctgagc
    39241 caaatggcgg tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc aaatgccctt
    39301 tgattgctgt tgctaaaata tcgatccgtt tatccacacc ttttgcccca acaccttgtg
    39361 caccataaat ctcgccagtc tctggatgga agatcaactt cagtaaaata tctgttgctt
    39421 ctggataata gcttgcatga tcttttcctg atacatgaat gacttcaaaa gacagttcgt
    39481 tttctttggc tgttctttca cttaaaccgg tggatgcggc agtcattcca aacgttcgta
    39541 taattgctgt tccaataccg ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa
    39601 cttgtctacc ttgacgattg gctggtgaag caagagaaat aagtgcatct tttccagtga
    39661 tttgctgttt tacaacgata gcatccccca ccgcataaat atcaggaata ttcgtttgat
    39721 aacgttcgtc tactaggata cccccgcgta tgcctaattc gattccagcg gtcactgcta
    39781 gctggttatc aggacgaaca ccaatggata aaataactaa atcagcaggg atggtacgcc
    39841 catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa
    39901 tcacttctac tccttgatga gacaattctt tttctataaa agctgccatt tcttcatcca
    39961 gtgttggaag aatatgtggt gcttgttcga tcaccatgac ttctaaacct cttcttttta
    40021 ggttttctgc catttccaat ccgatgaatc ctgcaccaac gatcacggca cgctttggct
    40081 gtttttctaa tgcatgcatc actttatcga tatctggtac atttctaata gaaaaaacat
    40141 tgtcggcttc tgttatccct ggaatcgatg gaacaactgg ttttgcacca ggagataaaa
    40201 ccaatgcatc gtactgttca atgtgttttt gaccctcatg tacgacttct accgttttgt
    40261 tttcgggaaa gatggctgtc acttcatgat ttggacgcac atctaaacaa aaacgagcgg
    40321 ataatgattc tggcgtttgg acaagaagat tttcccgttc actgatttct cccgaaagat
    40381 aataaggcaa gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa acaacgattt
    40441 ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga cattccacct gctacacctc
    40501 cgacaataac gattttcatc ttatttccct cctactgttc gatcaaacca ttgattcatg
    40561 ccaccacgta cattcactac atcataacca gatttcttta attctttagc tgcttgttta
    40621 ctcctcatcc ctgattgaca aatcacatag acggtctttt ctttgtctcc atcatatctg
    40681 tttatacttt gtaatggaac atttttagct ccttttatat gcccgccccg gtattctgca
    40741 ggtgttctaa catcaagtaa cgctggattt tccaaaagct tttcctttaa ttctgggact
    40801 gttatcgatt ttattgattg aaatagcaaa ttcattttct gcacctcatt tacattttta
    40861 taaatacctc atggggtata ttaaacagga gctttattaa tatgtcaacc agaatgactc
    40921 ttcatttttt tcactacttt ttatatttgc ataaaatacc gataaggggt attatctaat
    40981 tataaaaata gaggagaatg tgttatggag attgataata aaaatatcct gaaccgtttg
    41041 aaacgtacag agggacaaat tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt
    41101 atggatgtca tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg
    41161 atcgttgcag ataatctgaa aaattgcttt gagaaacctg atagtaatcc agaagaacaa
    41221 gcggctaaac tagaacaagc gatcaagatg attattaaaa agtaaaggaa tgatttcaat
    41281 gaaagtcgtt ttccatattg atgaattaga aaaatggtca gaaacaggga aaaacgtaaa
    41341 aaacctgata aaagcttctc cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat
    41401 taccggttat ctagactctg ccaatgcaga attcttagat ttgcaaggag tcgtttttca
    41461 cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa agttctttac ctgaacaagt
    41521 aatcgttgta ccagcaggtg tccttgattt agttgaactg caatcccaag gttatgctta
    41581 tatcaagcct tagtcagtag aggttgcgac agaagtgatc agcttcaaga aatttcggct
    41641 tatttttcga agaagctact tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc
    41701 tgcatcaagt
//
LOCUS       DACEDM010000015        40682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEDM010000015 DACEDM010000000
VERSION     DACEDM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.015"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            33..656
                     /locus_tag="IWO50_000758"
     CDS             33..656
                     /locus_tag="IWO50_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357899.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1105..2154
                     /locus_tag="IWO50_000759"
     CDS             1105..2154
                     /locus_tag="IWO50_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0357900.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2151..3032
                     /locus_tag="IWO50_000760"
     CDS             2151..3032
                     /locus_tag="IWO50_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0357901.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3035..3805
                     /locus_tag="IWO50_000761"
     CDS             3035..3805
                     /locus_tag="IWO50_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0357902.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4084..4938
                     /gene="aroE"
                     /locus_tag="IWO50_000762"
     CDS             4084..4938
                     /gene="aroE"
                     /locus_tag="IWO50_000762"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0357903.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5116..6279
                     /locus_tag="IWO50_000763"
     CDS             5116..6279
                     /locus_tag="IWO50_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0357904.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6396..6806
                     /locus_tag="IWO50_000764"
     CDS             6396..6806
                     /locus_tag="IWO50_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0357905.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6980..9208
                     /gene="topB"
                     /locus_tag="IWO50_000765"
     CDS             6980..9208
                     /gene="topB"
                     /locus_tag="IWO50_000765"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0357906.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9346..9663
                     /locus_tag="IWO50_000766"
     CDS             9346..9663
                     /locus_tag="IWO50_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357907.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9823..10059
                     /locus_tag="IWO50_000767"
     CDS             9823..10059
                     /locus_tag="IWO50_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0357908.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10056..10454
                     /locus_tag="IWO50_000768"
     CDS             10056..10454
                     /locus_tag="IWO50_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0357909.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11121..12065)
                     /locus_tag="IWO50_000769"
     CDS             complement(11121..12065)
                     /locus_tag="IWO50_000769"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0357910.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12361..13163)
                     /locus_tag="IWO50_000770"
                     /pseudo
     CDS             complement(12361..13163)
                     /locus_tag="IWO50_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13384..14598
                     /locus_tag="IWO50_000771"
     CDS             13384..14598
                     /locus_tag="IWO50_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0357911.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14620..16635
                     /locus_tag="IWO50_000772"
     CDS             14620..16635
                     /locus_tag="IWO50_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0357912.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16718..18091)
                     /locus_tag="IWO50_000773"
     CDS             complement(16718..18091)
                     /locus_tag="IWO50_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0357913.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18117..18401)
                     /locus_tag="IWO50_000774"
     CDS             complement(18117..18401)
                     /locus_tag="IWO50_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0357914.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18403..20418)
                     /locus_tag="IWO50_000775"
     CDS             complement(18403..20418)
                     /locus_tag="IWO50_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0357915.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20597..20902)
                     /locus_tag="IWO50_000776"
     CDS             complement(20597..20902)
                     /locus_tag="IWO50_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0357916.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(20991..22022)
                     /locus_tag="IWO50_000777"
     CDS             complement(20991..22022)
                     /locus_tag="IWO50_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0357917.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22229..23104
                     /locus_tag="IWO50_000778"
     CDS             22229..23104
                     /locus_tag="IWO50_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0357918.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23256..24098)
                     /locus_tag="IWO50_000779"
     CDS             complement(23256..24098)
                     /locus_tag="IWO50_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0357919.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24190..26067)
                     /locus_tag="IWO50_000780"
     CDS             complement(24190..26067)
                     /locus_tag="IWO50_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0357920.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26064..27485)
                     /locus_tag="IWO50_000781"
     CDS             complement(26064..27485)
                     /locus_tag="IWO50_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0357921.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEELRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27488..30877)
                     /locus_tag="IWO50_000782"
     CDS             complement(27488..30877)
                     /locus_tag="IWO50_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0357922.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31134..32525
                     /locus_tag="IWO50_000783"
     CDS             31134..32525
                     /locus_tag="IWO50_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0357923.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32598..33245)
                     /locus_tag="IWO50_000784"
     CDS             complement(32598..33245)
                     /locus_tag="IWO50_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0357924.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33253..33939)
                     /locus_tag="IWO50_000785"
     CDS             complement(33253..33939)
                     /locus_tag="IWO50_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357925.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(33945..35018)
                     /locus_tag="IWO50_000786"
     CDS             complement(33945..35018)
                     /locus_tag="IWO50_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0357926.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35240..35914
                     /locus_tag="IWO50_000787"
     CDS             35240..35914
                     /locus_tag="IWO50_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0357927.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            35919..37259
                     /locus_tag="IWO50_000788"
     CDS             35919..37259
                     /locus_tag="IWO50_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0357928.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37408..38394)
                     /locus_tag="IWO50_000789"
     CDS             complement(37408..38394)
                     /locus_tag="IWO50_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0357929.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39118..40149
                     /locus_tag="IWO50_000790"
     CDS             39118..40149
                     /locus_tag="IWO50_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0357930.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40258..>40682
                     /locus_tag="IWO50_000791"
     CDS             40258..>40682
                     /locus_tag="IWO50_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0357931.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        12638 a         7379 c         7646 g        13019 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aattggcgca gctggcgtag ataagttgta ttaatggtga
      901 gactttccca tagatacaac tttttagagt gcatttctaa agcttgtatc tatgctagac
      961 atgaacattg actgccgcat agattagaat ctatgcggtt ttttgtgtaa agacaatgac
     1021 cgctttggcc attgtctttt tttgtagact ggatacttgc ctgattataa taaataaaaa
     1081 gattgacttt gcaggaggaa aaaaatgaaa aacaaacgat taataacagt tgtagtaatt
     1141 attttagttt atttagcagg tgcatttatt tatggaaaag cctcaggcga aaaagaaagt
     1201 acttcgacga accagacaaa aaaaacaaca gttgttgggg tattgcaata cgtgagccat
     1261 ccggcattag atgagattta tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa
     1321 gaaggagaaa atcttgagat caaatttcaa aatggacaag ccgatcagag caagctagcc
     1381 acaatgagtc aacagctagt acaatctgat ccagatgcct tgattggaat cgcaacacct
     1441 gccgctcaat cattagcaaa cacgacttca tcgatcccag ttgtgttagg tgctgtaacg
     1501 gatcctgtgg gggctggatt agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt
     1561 gtttctgatc agccgcctgt tacttcacaa atcaagcttg cttctgattt gttgccaaat
     1621 gcaaaaaaag tcggaatctt gtatgcttct tcagaggata attcaaaata ccaagtagca
     1681 caagcagaag aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc
     1741 actaatgaga ttgcacaaac ggttcaagtg atgagccgac aagtcgattt tatctatgtt
     1801 ccattagata ataccattgc caatgcgatg caaacagtgg taaaagaagc aaacaaagca
     1861 aatatccccg tcattccatc tgtggataca atggtggaac aaggtggact agcaacaatc
     1921 gggatcaatc aatatcaatt agggcttcaa agcggtaaga tggcggcaga acttgcgagt
     1981 ggaaaagaaa aaccggaaac gacgcctgtt tatacatttg atcaagggga tacagtgatc
     2041 aatcaatctc aagctgatcg cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc
     2101 aaaatcatta cagatgactc gcagcaagaa acatctaagg aggatgacaa atgatcgtat
     2161 cagctatttc acaaggcatg ctttgggcaa tattaggatt agggattttc atgacttatc
     2221 ggatcttaga ctttcctgat atgacgacag aagggtcctt cccattaggt gctgctgtct
     2281 gtgtgacagc tattactcat ggggtagcgc cgattgtagc tacattatta ggcgtaggtg
     2341 ctggcatgat agcaggactc gttacggggt tactttatac aaaaggaaag ataccggtta
     2401 ttttagcagg gattttagtg atgtcaggat tgaactcagt tattttgttt gtgatgcgaa
     2461 caccaaatct ttctttattg aatcatccga ttctacaaga cagcttccat cgctttgggt
     2521 tacctgatta ctttgacaca gtccttcttg gcttcatctc tttgggcatc ttgattgctg
     2581 tactgctatt cttcttcaat acagatttgg gtcaaggcta tattgcgaca ggagacaacg
     2641 aaacgatggc tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta ggtttaactt
     2701 tgtcgaacgg tgtcattgca ttatccggtg cgctgattgc ccagaacgac ggatatgcag
     2761 atgtaaataa agggattggc gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag
     2821 tgatcttcca agaattgacg ctagcagaaa gattgattgc tattgttgtc ggcagtatca
     2881 tttatcagct attgatttta gtagtaatca aactagggtt tgacacgact tatctgaagt
     2941 tgttctccgc tatcatctta gctatttgtc tgatgatccc gcaattgaag aaagcactga
     3001 acttaaaaac gggttttgag aaggaggcat agtaatgact gatatattga caatcaaaaa
     3061 tggcagaaaa gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg atgaaattaa
     3121 ccttacaatc agagaagggg acttcatcac tgtccttggc gggaatggtg ctggaaaaag
     3181 cacactgttc aatacgatcg ctggtacgct tcaactcagt caaggatcga tctcttttaa
     3241 agatcagctg atcacaaaag attcagaaga aaaaagagca cgattccttt ctcgagtttt
     3301 ccaagacccc aagatgggaa cggctcctcg gatgacagta gcagaaaatt tgctacttgc
     3361 acaaaaaaga ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga
     3421 ttttttcaga ctatgtcaag aagtcggtaa tggcctagag caacacttgg atactccagc
     3481 gggtgaatta tctggtggtc aacgccaagc attaagcttg ctgatggcga cgatcacagc
     3541 tcctgagtta ctattattag atgaacatac tgcagcttta gatcctaaaa cagctaaagc
     3601 attaatgcga ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac
     3661 gcatcgaatg gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa
     3721 aatcgttcaa gatatttctg aaaaagaaaa gcaacatttg acaatgggcg accttttgca
     3781 gttctttgaa gaagtagtgg attgatttcg acagaaaaat tcctttcagt agaaggactt
     3841 gaaaactcat tgacatcttc ctttgattca tcctatactt aaaacaataa acaattaaac
     3901 atatttgggt gttttgtagg atcacgtagt caatgatttt aacaattaaa tagaatattc
     3961 tttgctctga caagtaaacg atgagaaaag aatttgctac tttgccacct agacgtttcg
     4021 tactttttcg tatcaaacgc taagtggctt tttttattga agtaagaaca aaggaggaat
     4081 aagatgattt ctggacaaac caaattagct gggctctttg ctgatccggc ttctcacagt
     4141 ttatccccat tgatgcacaa tactgctttt gaagcaaaca acatcgatgc ggtgtattta
     4201 gctttccaag taaatccaat gaatttgaag caagcagtcg aaagtattcg aacttttgat
     4261 atgttaggtg taaatctttc gatgccgaat aaaacagcgg ttattcctta tctggatgag
     4321 gtaagccagg aagcgcaatt gatcggagca gtgaatacga tcgtgcacga aaagggtcgt
     4381 ttgattggct ataatactga tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt
     4441 tcgatcaaaa agaaaaaaat gactctactg ggagctggag gtgcagcaaa ggcagtcact
     4501 gttcaagcag cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca
     4561 ttcgctcaag taaaagaaac attcgaacaa ctcagctatc gaacttcttg taagataaaa
     4621 gttcgtgact ttgccgatac gcatcaatta agaaaagact tgaaagaaag ctgtcttcta
     4681 gtcaatgcaa cagatatcgg gatgggaaag aaaatcaatc agtcaccgct cttagatata
     4741 agcatgatgc atgagaatct agcggttttt gatttgattt atgcacctag agaaacacgt
     4801 ttattaaaag aagcaaaaaa agtaggagca aaaggataca atggacttgg catgttgatc
     4861 taccaaggag caatcgcgtt tgaattatgg acaaaggaaa agatgccgat ccatgtgatt
     4921 gaaaagctgt ttgattaaaa aaggataata gataaagatc ttttattctc gcataaacgg
     4981 tctcaagaaa agtaatcctt tatgtatcga agcatttatt tcgttatttt ttattaaatt
     5041 atcaaaaaat gataggcttt tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa
     5101 cgaggaggaa ccacaatgag tcggatggat cgctataagg acatccataa gaaggcgaaa
     5161 cctctgaagc gggatacggg gagtattttt gctagaagag aacgaaaacg aacgcgtatg
     5221 gaaaatgagg aagatgctac ccgtatttat gaaagagagc agcattcatt tgaacatttg
     5281 aaccaatacg agcaacagat atacagagat gcaccaagag cagaagaaca acaaaaaaat
     5341 aaaaaatttt tcaatcggca aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg
     5401 tggaaaaaaa tcattctatc gattttgctt gttttgatcc tttattcagg tatctcattt
     5461 tttattggta aatatgtagc tgaacatgac acaagcttag aggcagcgac ttcagaaacc
     5521 tttcatgggc aaaaagcgac gaatggtgcg cataatattt tgatccttgg aagtgataca
     5581 cgcggagaag atgccgggcg agccgatacg atcatggtct tacagttgga tggtccttcc
     5641 cataagccga aactgatttc ttttatgcgt gacagcttcg ttacgatccc aggggtagga
     5701 caaaataaga tcaattcagc ttatgcatac ggaggagccg atctagtgcg tcaaacttta
     5761 gtagaaaatt ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa
     5821 aaagtgatcg atgccttatt catgaacgga gtgaagatcg atgcagaaaa agatctgaat
     5881 ctggatggtg tagatattaa aaaagggggc cagaagatgg atggacatgt tcttttgcag
     5941 tacgcacgtt tccgaatgga tgaacaagga gactttggcc gtgtcagacg ccaacagcaa
     6001 gtaatgaacg ctattttcag ccaattgaag aatccattga atctgatccg tgcaccttat
     6061 gcagctggta aagctttagg atatacatcc acagatgtca ctgcttggtt cttgacgaaa
     6121 aacctctttt cgattgcacg aggagctggt ggcattgatc gaataagtgt gccagtagac
     6181 ggttcgtgga attatgggca aagtgattat gctggcagta ttcttgaaat tgataatcaa
     6241 atgaatcaat ctgctattac agattttttg aataaatgat cataaaaaag atcaagaaga
     6301 agctgcgcaa ggcttttgtc atgaatcaat caaacgattc actcgtaaaa aacgctttct
     6361 tatggtagat ttaaccaaga gaggagctgt ttacattgga tatctatctg aacttcaaaa
     6421 atgagtcgcg tgaagccatc gctttttatg aaaaagtttt tcagactgaa gcatcaggta
     6481 tcaccacata cggtgaaatg cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa
     6541 atctaatcac gaatgcacgg ctgaacattt atgatacatt agttatgttt gcagatacgc
     6601 cagaaggaat tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc atccagacaa
     6661 gaaatgaaga agaaacgaag cgcctatttg aaagcttagc tgagggtggt aagattgaga
     6721 tgccactgga tgctgttccg tgggctcagt tgtatgggat ggtaactgat cgatttggca
     6781 tcaactggca gattaaccaa gagtgacagc taaagatgaa tattttctgg aaacatcaag
     6841 cagcaaacgg atcatcgttt gtttgcttga tgtttttgtg tctatttcga ctatacggta
     6901 tctttttttt attgccagat gcgaaaatat gttccataat agagaagtcc gcaaaaaatt
     6961 aagtaacgga ggtcagaaga tgaagacggt gatactagct gaaaaacctt cacaagcaaa
     7021 agcctatgcc gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg aaatcagcga
     7081 tccattgttt gctggtgaag taaccatcac ttatggtttt ggacatttgg tagatatggt
     7141 tgctccagga gcttatgaag agcgttgggc aaaatggtct ttagaaaatt tgcctatttt
     7201 cccagaaacc ttccgttacg aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa
     7261 acgagaattg cagtcagccg ataccatcat tattgcaaca gatggtgatc gagaaggtga
     7321 agccatcgcg tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa agaactattt
     7381 acgtttatgg atcaattctc ttgaaaaaga agcaatctat gaaggtttta agaatctcca
     7441 acctggaaag aactattttc caaaatataa agaagcgcaa gcaaggcaga acgctgattg
     7501 gctgattgga atgaatggaa gtccattata tagcttgcta cttcaacgaa aagggattga
     7561 tggcagtttt tctcttggtc gtgtacaaac accgacactc tatatgattt atcagctgca
     7621 agaagaaatc aaacacttca aaaaagaaac gtactttgaa ggagaaggtt taattacgac
     7681 aaacaaaggg cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga
     7741 gttaacgaag gaaatagaaa aacttggagc gcatttaggt aagcaaaacg gacggattct
     7801 tgaagttacg aaaaaggaaa agaggttgca tagtccacgt ttattttcgc tatctagttt
     7861 gcagacaaag atgaatcagc taatgaaagc aagtgccaaa gctacacttg atgctgctca
     7921 aggattgtac gaaagcaagt ttctaagcta tccaagaacg gattcctttt acattacaga
     7981 aaatgagcat cagtatctgg ttaaacatct gcaaaattat aaaaaatttt taggaataga
     8041 agaagtacag acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca
     8101 agaacatcat gcaattattc cgacaaagac gattccgact cctgcacgct ttgcgaagct
     8161 ttccaaatta cagcaagcaa tctatttgca agttttgcgc actactgtgg ctatgtttgc
     8221 tgcagattat atctacgaag agacgaccgt aatgacgggc gtacagcaat taaagctaaa
     8281 aagcatcgga aagatcccat tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa
     8341 aaagaaagaa gctcagactc ttcctcctat cgtagaaaat gaagaagcag aagtagaact
     8401 gaaaagtatg gagaaagaaa cacaacctcc aaaaccctat accgaaggaa cgttgatcac
     8461 agcaatgaaa actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga
     8521 agtagaagga atagggacag aagctacacg agccaatatc attgaaaatc ttaagcagaa
     8581 gcattacatc gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa tcactctttg
     8641 taaggcagta gccaatgaac cattactgac gagcgcagaa atgactgcgc gctgggaagg
     8701 gtacttgaga aaaattggta aacaagaggg gacaccatct gtttttttag agaatatcaa
     8761 aaaattcatc ctacatttat tggcagaagt tcctaatcaa atcgacaagg tagacttttc
     8821 agatgagatt agcgggacac gtcagataaa agcgatggag aagaaaaacg atcaattagg
     8881 aagatgtcca aaatgtaaaa agggaatagt catgctttat cctaaaatcg ctacatgctt
     8941 gaatccggaa tgcgacttca aattgtggcc aacagtagct aaaaagaagc tgactaaaac
     9001 gaatctcaga gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg
     9061 taaaaaaggg aaattcgatg cagttttagt attaaaagaa gatatgacaa tcggtttttc
     9121 ttttccatgg atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag aaacatcaag
     9181 aaaaaataat gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga atatttattc
     9241 cgaaaagtga gaatataaat accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac
     9301 gttggggtag gctgatttta tatagatagc aaggagtttt tttatttgaa aaaagtgcat
     9361 tcatttttaa attatcttat gggagcaatc acgattgtgg ctgtgtacca agcatttcta
     9421 aagtatcctg attacgggac gaagttgttg tcattgacgc acaatcaaac agcatattca
     9481 ccaacgttga ttttatcgac tttgactgca ggagctaaaa gcgctactta tttttttcat
     9541 aaaacgacaa gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg
     9601 gctatgatca ttatttattt tctaccagtt attacacaag tatttcatca atattttggt
     9661 taagaatacg agagtttgtg acaaaagtca tgtcacaaac ttttttcttt gtcatttttt
     9721 cgtaagaaaa agcaaaataa aagtatatac attgattaaa agtatatact tggatataat
     9781 gtacttacta tagaatgaga atggtataga gggtggcgaa atatgaaaaa aatgaaaaag
     9841 tataaaatta gaaaatctgg caatagcgat gtaacaacta tcccaccaga agtaaaagaa
     9901 ttcatgggtg tgcagacagg tgacgcaatc agttatgttt ttcaatctga tggttctgta
     9961 cgaatgatca aagcacaaga agaacctgat attgattcat tagttgattc gatcatgaat
    10021 caatatgaag acgcgttaaa agatttggtt gatttatgag gtatctttcg actaaagaaa
    10081 ttatcaaaat caacgcgaaa gttattttac gtcactcgcc aggcgaaatg atcggtgtaa
    10141 aagatgcaaa tgcattagat atggccgtca aacagccttc acaagcagta ttcaatcaag
    10201 aactctatcc tgaaatctat gaaaaagcgg cgattttagc aattaattta gctaaaaaac
    10261 atccttttca taatggcaat aaacgaactg ctcttgttgc catgttgttg tttcttcaat
    10321 tgaataattg tccagttgct ttttctcgtc aagaagctgt tgattttatc ataatgatta
    10381 ccactagttc attggatttc gattcactaa agtccaagat aaccagttat ttacgacaaa
    10441 ccgcaataaa atgaacgaat taaaaacttt atttctcaat tagatcagca tgtcattaag
    10501 gaatatgctg atttttttat gtttaatcat acaaaatagt tttttcataa aaatatggaa
    10561 aatatggatt aattttgaaa taattgattt aaaaggaata caaaagagtg aatagtagga
    10621 aatagtggtt tagaactagt gggctgattt tttatatatt ttagtttaca taatatatat
    10681 tatacaaagt agatttaaaa aatgataaac ctctctcgaa cggtttttta tcaaaaatcg
    10741 tcatttttcc atcaaaatct gcttatgcaa aattataaaa aaattttgca gtttttctta
    10801 ctttttttgt cgtttaattg aatgataaaa accagtttct ttattcagtt aatcaaatat
    10861 gaacgaatta gatatgaatc attgtatttg ccgttgcgga aggatttttt aattaaccac
    10921 agataaacat tgataacgat tcgcttattg taatcaacag attaaatttc atatgttcat
    10981 atgtacggat acattttcgg accgattaat tttaaccatt ctgtttattt gcttacctat
    11041 tagatatcaa taaaaaactg cttaacagct atttcagctg ttaagcagtt ttaggatttt
    11101 tagttcatat tcattgtttc ttataataca gtgttcaata cttctcttaa agcacttgtt
    11161 gatttgctga acattgcttt ttcttcttcg ttgattgata attcaacaac ttcacgaaca
    11221 ccattttcgt caacgattga tggcacacct gtaaagatat ctttttcgtc gtattcacca
    11281 gttaaataag cagatacagg caatacagct tgttcgttat tcaagatagc ttttacgata
    11341 cgtgcagtac tcataccaat accatagtaa gttgcttttt tacgatcaat gatctcataa
    11401 gcagcattac ggactttatc agcaatcaca tctaattcat cttgggcaat acgatggtct
    11461 ttttctacaa tttcaaacac tggtttacca ccgacagttg tatgtgacca tgcagcaact
    11521 tctgaatcac catgttcacc taagatatag ccgtggacgc tgcgtgggtc aacttttaat
    11581 ttcaatgcga tttctttacg gaaacgagtt gtatccaatg ttgttccagt accgataaca
    11641 cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc tactgggttt
    11701 gaagcaacaa cgataatacc gtcgaatcct gatcccatga tttcttttac gatttggcgc
    11761 atgattgacg catttgtttt aactaaatct aaacgagatt gaccaggttt ttggttgata
    11821 ccagctgtga taacaacgat atcagcatct ttacattctt catagccacc tgaccataca
    11881 gccacatttt catcgcccca agccatacca tctaaaaggt ccaatgcttc cccttctgct
    11941 ttttcttgat tcacatcgat caatactaat tcattagaga ttccttggtt gatcattgca
    12001 taagcgatac ttgttccaac aaaacctgta ccgacgatta ctactttacg acttgttttt
    12061 ttcatgaata aacagctcct tttcataact atttgttact ttcatgaaaa gtatagtctt
    12121 gtttattcat tttatcaaca tgttaattat aatagttgtt attataattt ttgatggaaa
    12181 cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata tcccctgata
    12241 atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca acgatataat
    12301 aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa gattttcttc
    12361 ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga ataatttgaa
    12421 taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg aaaaactcgt
    12481 ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt cttgaaagga
    12541 agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc caaaatgagc
    12601 tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta atactctaag
    12661 catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta ataaatgagt
    12721 caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat gactgtatgt
    12781 atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg tttttccgcc
    12841 gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta tcaaaaaaga
    12901 agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata aggtttcttc
    12961 ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc tttgaggtgt
    13021 ttactgatat gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc
    13081 tgaagatggt aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt
    13141 tcgattgatt cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc
    13201 tcctttttgt aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa
    13261 agctattttt ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg
    13321 ctattgagag aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg
    13381 aacatgctta tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg
    13441 acttctgctg gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt
    13501 tctttatctg ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt
    13561 ttgattagta gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta
    13621 gtgattgcat cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta
    13681 gcttcaagag ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg
    13741 attgctgcct tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt
    13801 caaggatttg gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg
    13861 caatatacct ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt
    13921 gttcctacac ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta
    13981 atcaaaacga atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta
    14041 tataatgctg tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa
    14101 actaatgcag cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt
    14161 tttggctggc ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg
    14221 ggaatcggct ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg
    14281 atcctaacag gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc
    14341 caagcgccca agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta
    14401 ggtactttca ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa
    14461 acacttgctg ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt
    14521 ctagtagtac acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa
    14581 caattgacaa caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa
    14641 gatagatggc tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc
    14701 caacgggaag aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg
    14761 aacgtcaagt ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc
    14821 caaggagttg ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact
    14881 tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg
    14941 atgtttatcg agggagacga agtaaaatca gatattggct ggacagcaag taattttatt
    15001 gaagaatatc cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa
    15061 atctattatc aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc
    15121 ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat
    15181 atcgaatcta tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat
    15241 tgctactata agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc
    15301 tatttgttta taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt
    15361 ttatctcttt cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg
    15421 tcagtatcag aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa
    15481 cgagatcgct ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt
    15541 ttttttgacg aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc
    15601 aagcagcata tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac
    15661 cactatatga tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca
    15721 ttaatgagat acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa
    15781 gattggatat ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa
    15841 gtcttattac tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca
    15901 tatggagaga acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa
    15961 caaaacctat ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa
    16021 tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct
    16081 aacaaagacc aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag
    16141 attactactc catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg
    16201 aatatcgtct accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta
    16261 actttctggg aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga
    16321 gatccttatg gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga
    16381 cgctatttta tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct
    16441 aatctagatt catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg
    16501 cttgaaaaaa caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa
    16561 atgaatggta aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca
    16621 aaaataaatg cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt
    16681 ctgatattgt ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa
    16741 tgtttgttct ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga
    16801 tgattccaac tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg
    16861 gtactagata atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg
    16921 gaaggataaa taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg
    16981 cccctcttct accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat
    17041 gtcctactac cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata
    17101 gtgttcctac aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa
    17161 aaacgatagg aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt
    17221 ggaatgctgg aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc
    17281 cagctgtgaa tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta
    17341 gttctctttc aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca
    17401 tcaagagcat gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta
    17461 tgtcctctgt agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat
    17521 aaccgatatt tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa
    17581 acggttgaca aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata
    17641 agatcatcca caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag
    17701 aaacataaag cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa
    17761 gtaaaatatt cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt
    17821 gcaaagcaat cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg
    17881 tttggaacat cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca
    17941 gtactaggaa tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct
    18001 tctgaagaac gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga
    18061 ttgacacaaa caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat
    18121 tcttttgaca agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc
    18181 aaaggtacga gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg
    18241 aagaaataat cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac
    18301 tcgtccacat ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa
    18361 cttgttccta atccactttg acatactgca aaaattttca tatcattcgt ctccttttct
    18421 tatgatcgct aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt
    18481 attagaaagt agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc
    18541 agctaaacag atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg
    18601 ttgaagtttc agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg
    18661 taatgcgatg tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat
    18721 agctttgata taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt
    18781 gattgcttct tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa
    18841 ttcagatagc ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt
    18901 taatacttga tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag
    18961 cgccgacaac aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg
    19021 tacttgattg atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag
    19081 agactttttc ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt
    19141 ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa
    19201 taattgctga agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat
    19261 cacggctctt atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa
    19321 tagtatagtg aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa
    19381 cggaagtaat gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc
    19441 gatcatcaca ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg
    19501 ataaaagaca ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc
    19561 catgcgatgg atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac
    19621 atctccttca acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg
    19681 ttcggaatag ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc
    19741 caacagttca gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa
    19801 actcaaataa taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga
    19861 aatcgtttta tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt
    19921 taaatacgtg agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt
    19981 tgcttctctt cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata
    20041 cgacagctga caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac
    20101 tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt
    20161 cgaataaacc atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa
    20221 tcgagtaata tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat
    20281 acgggtatca tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt
    20341 tacttcgatg atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata
    20401 actaattttg tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct
    20461 tttaaatcga ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa
    20521 cgatttctat cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg
    20581 aattgatttc gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt
    20641 tttctttgac atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag
    20701 gctttgtttc ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt
    20761 caccagagtt tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc
    20821 ctgctaaagc cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt
    20881 tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta
    20941 caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt
    21001 ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt
    21061 tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag
    21121 acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca
    21181 tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca
    21241 ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc
    21301 cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt
    21361 atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct
    21421 ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga
    21481 acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca
    21541 tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata
    21601 tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt
    21661 ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct
    21721 gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt
    21781 gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc
    21841 tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc
    21901 cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt
    21961 gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc
    22021 atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat
    22081 ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg
    22141 aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat
    22201 cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga
    22261 gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc
    22321 agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat
    22381 tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg
    22441 ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc
    22501 aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat
    22561 aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa
    22621 caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt
    22681 ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc
    22741 ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg
    22801 tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt
    22861 gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat
    22921 atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga
    22981 ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg
    23041 caacttgaaa aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac
    23101 ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata
    23161 tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac
    23221 gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac
    23281 gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca
    23341 tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca
    23401 tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta
    23461 caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc
    23521 ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata
    23581 tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt
    23641 cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct
    23701 tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa
    23761 tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta
    23821 tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct
    23881 tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca
    23941 ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc
    24001 agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc
    24061 atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact
    24121 gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt
    24181 gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag
    24241 aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt
    24301 acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga
    24361 ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt
    24421 cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact
    24481 tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga
    24541 tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc
    24601 tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc
    24661 agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc
    24721 ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa
    24781 tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa
    24841 tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata
    24901 taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt
    24961 atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa
    25021 tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt
    25081 caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg
    25141 tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc
    25201 attatcagga gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat
    25261 caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg
    25321 accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt
    25381 gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc
    25441 tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc
    25501 attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt
    25561 ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc
    25621 ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt
    25681 ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac
    25741 tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt
    25801 atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa
    25861 tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg
    25921 taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac
    25981 tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat
    26041 aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac
    26101 cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg
    26161 tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg
    26221 tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt
    26281 aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg
    26341 gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt
    26401 gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg
    26461 aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta
    26521 ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg
    26581 ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg
    26641 gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg
    26701 tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt
    26761 aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga
    26821 tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc
    26881 gtaaaatcat ttctaagctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt
    26941 tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca
    27001 ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca
    27061 atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg
    27121 ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac
    27181 gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga
    27241 tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac
    27301 gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt
    27361 gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc
    27421 tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt
    27481 ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac
    27541 aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg
    27601 gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca
    27661 gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag
    27721 taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag
    27781 ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt
    27841 ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt
    27901 cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc
    27961 atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga
    28021 tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga
    28081 ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt
    28141 ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc
    28201 ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa
    28261 cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt
    28321 ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc
    28381 ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca
    28441 atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa
    28501 tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat
    28561 ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat
    28621 ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg
    28681 cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat
    28741 ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt
    28801 taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa
    28861 tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact
    28921 ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt
    28981 cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg
    29041 gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt
    29101 cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag
    29161 tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga
    29221 atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag
    29281 cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat
    29341 cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga
    29401 gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt
    29461 ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata
    29521 gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat
    29581 cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct
    29641 gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc
    29701 catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac
    29761 catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca
    29821 tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat
    29881 tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct
    29941 ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga
    30001 gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca
    30061 atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg
    30121 ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta
    30181 taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc
    30241 ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag
    30301 gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg
    30361 ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta
    30421 ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat
    30481 cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat
    30541 cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat
    30601 aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa
    30661 tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt
    30721 tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat
    30781 ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat attatcagca
    30841 tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt
    30901 tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt
    30961 attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac
    31021 caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa
    31081 gggttgaggg agaaaaacat atgatcaggt tgttggaacg aaactataga aagatggaaa
    31141 aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac
    31201 gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg
    31261 gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg
    31321 taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat
    31381 taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca
    31441 tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt
    31501 tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt
    31561 tgcgtatctt tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc
    31621 caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt
    31681 ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca
    31741 tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga
    31801 taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca
    31861 gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga
    31921 aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat
    31981 ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt
    32041 ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg
    32101 cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat
    32161 tttattccac tctactgaat cagaatctga tttattattt atctagctat atcttttgga
    32221 tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca
    32281 gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg
    32341 tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc
    32401 ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata
    32461 tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt
    32521 tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata
    32581 cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag
    32641 tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt
    32701 acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa
    32761 tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct
    32821 ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag
    32881 atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa
    32941 tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt
    33001 ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc
    33061 gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa
    33121 aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa
    33181 gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt
    33241 gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg
    33301 ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt
    33361 ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc
    33421 caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc
    33481 aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga
    33541 ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt
    33601 aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct
    33661 ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc
    33721 agttagtgag tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga
    33781 aatggtaagg aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc
    33841 ttcctttaca taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa
    33901 tgttttggta acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct
    33961 gtaattgggt ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca
    34021 atgatcaaaa tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg
    34081 cttggtaaaa tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc
    34141 ccaacaataa aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt
    34201 gctttcatta ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg
    34261 accacggcag caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg
    34321 gcagaatagc caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc
    34381 gatgcttttt ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt
    34441 tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac
    34501 gcatagatga cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa
    34561 ggatcgtcgt aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga
    34621 ttttgagaga tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt
    34681 aataaaaatg cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg
    34741 attgctccgc tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct
    34801 cctcttgtta attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct
    34861 tgtgcgtccc aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct
    34921 gacaacatga atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt
    34981 aatttagaat agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag
    35041 attgatagtg acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac
    35101 ttgtgttgtt tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca
    35161 tgagtcataa aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc
    35221 aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc
    35281 caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag
    35341 tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat
    35401 gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc
    35461 agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg
    35521 tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc
    35581 attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag
    35641 ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa
    35701 agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca
    35761 gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta
    35821 tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt
    35881 acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa
    35941 agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt
    36001 tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat
    36061 tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag
    36121 gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat
    36181 taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg
    36241 agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg
    36301 atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg
    36361 ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca
    36421 tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta
    36481 tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta
    36541 gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg
    36601 cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta
    36661 ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga
    36721 cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc
    36781 agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac
    36841 catttcaaga gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat
    36901 tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg
    36961 cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag
    37021 aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga
    37081 tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc
    37141 ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca
    37201 attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat
    37261 gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat
    37321 aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac
    37381 ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac
    37441 atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac
    37501 atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac
    37561 atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt
    37621 tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac
    37681 cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt
    37741 tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc
    37801 tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc
    37861 aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa
    37921 gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc
    37981 cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg
    38041 ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg
    38101 atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt
    38161 atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt
    38221 ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt
    38281 ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa
    38341 tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat
    38401 tcctcctagc aaatagattt atgattggta tattgttact tataaattta ttctatagga
    38461 gacgattgtt ttctacaaat atttagattg gaatttgatt aaaacaattt aattgaaatt
    38521 tgaaaacatt tatgttaatg aaagtcatat tctaataaaa tgtcatttgt ttctatatga
    38581 atttttatga aaaatttcat ataatttcca ctcgttgtga tggtttaatt tatccaatcc
    38641 atcaataagc aaacaactta tataaaaaag gattaattct caatataatt gctcctacca
    38701 tagaataaat cttcatgtaa agtaatgaag taccaactat ttctaatact tctttaagaa
    38761 ttatcccaac aatatcgtta gacaaaccat tattgaataa atggagagcc aatcgattct
    38821 gaaaaaatgt tttctaattt ttcggaaata aaagaaagag cctcctgcca ttagaacgaa
    38881 ccaaatccat ttcattgctc atgaagtaac cacatagcta aaaagaggat aaatcaatta
    38941 aaagatcaaa agaatgactg cactaataaa ataactaaaa atgtatcttc ttacttcata
    39001 tcttttagct agtacttagt taaaaatttg cttcctaaga tcaagacaaa ctgtctatct
    39061 tcatttgatg aaatgcggta aaatatagaa aatgaacact attggaggga atttgtaatg
    39121 gacagcaaaa ctagaaaaca actgcaagaa aagttaaagc aacgtacaca cttgctccct
    39181 tccgtctttc aacagttttt agaagaaaat cgttcttccc tttcattagg cagtcgctat
    39241 gaatacgcaa aagattttca tctgtttact gattatttga gaaaagtatt ttctgaagaa
    39301 gtggatgatc agctgattat ccatttagaa aaacaagatt atcagaattt cttagctaaa
    39361 atctatcgac atactcgttc gttccaaact acagcgaacc aagaaacgga gcaattgttt
    39421 gaaaatagct attacgggat ttcaaggaag caatattcat taaatcattt tttacgattt
    39481 ttgtatcaaa aaggactgat tgaagaagag atttctcttt tgggtacaag tttaccggaa
    39541 acaaccaaag cagctccgcg atatatcgat gcagaacttt tcaaagattt tgagcatttg
    39601 tttatcccga ttgaaggctt tcaaacaagc agagaagcta gctttgaaga aaagaataga
    39661 ccgaggaacc ttgcaattac tgccattctt ctgtattgcg gattaaagat ccatgaagta
    39721 gtagaactta aacgtaaaga tgtatctttg accaagcaga ttcttaatct gaatagaaaa
    39781 gaaaacagac tgaataaagt accaatacca aaagaagcga tgccatttct ggagaactat
    39841 tttaagttaa cagcacaaaa tacggacgaa aattcttttg tttttcgttc ctcacatgat
    39901 cagcaaattt ctcctcggac tattcgccaa attttgagca aaattacagt atataaaaac
    39961 gtatcgccag aactatgcag aaaaacatat cgttattatg cagaactata tttagatgat
    40021 gctgacgcgg taaattattt atgtggtaat cgatacctct catcccattc ttttcaagat
    40081 atctgggaaa agatggttga tttttcttat cacgaattag cggcattcgt tcgggtttct
    40141 tccatataaa acaaatgtat gatctttttt tcacaataaa ctatgttatt atataaagga
    40201 ttatgttacg attatcttga atttgtatat tacttaacaa aagataatgg agtgtgaatg
    40261 atggagacca taacgtattc ccctttgaac ttatttacga attttcaaga agctgcagct
    40321 aacttttccg agaccccgat tatctttgat gagccacttt cagctttccc agaattagga
    40381 cttgaaacaa cttacaaaaa atgctgcgaa agtgtgttga aacgggctta tcaattggct
    40441 catcttggag tgaaatccgg agacaaagtt attatttata agagttccgc ttttgacaca
    40501 taccttctag ctgtttctgt gtcttacctt ggtgcagtgc ctgtcatgac ttcttatcat
    40561 ctaccaacaa caacaatgga agtatttatc gatcgtttag aagacccttt cattttattt
    40621 gatgatgaga caaaaaagcg agtgagagaa atttcaaatg gaacaaaatc aaaacagatt
    40681 cc
//
LOCUS       DACEDM010000016        40385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEDM010000016 DACEDM010000000
VERSION     DACEDM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.016"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..212)
                     /locus_tag="IWO50_000792"
     CDS             complement(12..212)
                     /locus_tag="IWO50_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0357932.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(517..1749)
                     /locus_tag="IWO50_000793"
     CDS             complement(517..1749)
                     /locus_tag="IWO50_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0357933.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2003..2572
                     /locus_tag="IWO50_000794"
     CDS             2003..2572
                     /locus_tag="IWO50_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0357934.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2750..3190)
                     /locus_tag="IWO50_000795"
     CDS             complement(2750..3190)
                     /locus_tag="IWO50_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0357935.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3348..4112
                     /gene="map"
                     /locus_tag="IWO50_000796"
     CDS             3348..4112
                     /gene="map"
                     /locus_tag="IWO50_000796"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0357936.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4144..5067
                     /locus_tag="IWO50_000797"
     CDS             4144..5067
                     /locus_tag="IWO50_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0357937.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5143..6282
                     /locus_tag="IWO50_000798"
     CDS             5143..6282
                     /locus_tag="IWO50_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0357938.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6275..7075
                     /locus_tag="IWO50_000799"
     CDS             6275..7075
                     /locus_tag="IWO50_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0357939.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7075..7902
                     /locus_tag="IWO50_000800"
     CDS             7075..7902
                     /locus_tag="IWO50_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0357940.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7880..8614
                     /locus_tag="IWO50_000801"
     CDS             7880..8614
                     /locus_tag="IWO50_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0357941.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8714..9580
                     /gene="rfbA"
                     /locus_tag="IWO50_000802"
     CDS             8714..9580
                     /gene="rfbA"
                     /locus_tag="IWO50_000802"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0357942.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9594..10166
                     /gene="rfbC"
                     /locus_tag="IWO50_000803"
     CDS             9594..10166
                     /gene="rfbC"
                     /locus_tag="IWO50_000803"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0357943.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10188..11216
                     /gene="rfbB"
                     /locus_tag="IWO50_000804"
     CDS             10188..11216
                     /gene="rfbB"
                     /locus_tag="IWO50_000804"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0357944.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11314..12165
                     /gene="rfbD"
                     /locus_tag="IWO50_000805"
     CDS             11314..12165
                     /gene="rfbD"
                     /locus_tag="IWO50_000805"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0357945.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12200..14233
                     /locus_tag="IWO50_000806"
     CDS             12200..14233
                     /locus_tag="IWO50_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357946.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14277..15557
                     /locus_tag="IWO50_000807"
     CDS             14277..15557
                     /locus_tag="IWO50_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0357947.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15767..16573
                     /locus_tag="IWO50_000808"
     CDS             15767..16573
                     /locus_tag="IWO50_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0357948.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16585..17805
                     /locus_tag="IWO50_000809"
     CDS             16585..17805
                     /locus_tag="IWO50_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357949.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17795..19381
                     /locus_tag="IWO50_000810"
     CDS             17795..19381
                     /locus_tag="IWO50_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0357950.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19420..21558
                     /locus_tag="IWO50_000811"
     CDS             19420..21558
                     /locus_tag="IWO50_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0357951.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(21926..22909)
                     /locus_tag="IWO50_000812"
     CDS             complement(21926..22909)
                     /locus_tag="IWO50_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ0357952.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23287..24678
                     /locus_tag="IWO50_000813"
     CDS             23287..24678
                     /locus_tag="IWO50_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0357953.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24694..25734
                     /locus_tag="IWO50_000814"
     CDS             24694..25734
                     /locus_tag="IWO50_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0357954.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25753..26838
                     /locus_tag="IWO50_000815"
     CDS             25753..26838
                     /locus_tag="IWO50_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0357955.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26871..27833
                     /locus_tag="IWO50_000816"
     CDS             26871..27833
                     /locus_tag="IWO50_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0357956.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            27826..29253
                     /locus_tag="IWO50_000817"
     CDS             27826..29253
                     /locus_tag="IWO50_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0357957.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            29255..30325
                     /locus_tag="IWO50_000818"
     CDS             29255..30325
                     /locus_tag="IWO50_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357958.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            30312..31733
                     /locus_tag="IWO50_000819"
     CDS             30312..31733
                     /locus_tag="IWO50_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0357959.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            31746..32753
                     /locus_tag="IWO50_000820"
     CDS             31746..32753
                     /locus_tag="IWO50_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0357960.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            32765..33769
                     /gene="neuB"
                     /locus_tag="IWO50_000821"
     CDS             32765..33769
                     /gene="neuB"
                     /locus_tag="IWO50_000821"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0357961.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            33766..34914
                     /gene="neuC"
                     /locus_tag="IWO50_000822"
     CDS             33766..34914
                     /gene="neuC"
                     /locus_tag="IWO50_000822"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ0357962.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            34887..35564
                     /locus_tag="IWO50_000823"
     CDS             34887..35564
                     /locus_tag="IWO50_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0357963.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            35554..36696
                     /locus_tag="IWO50_000824"
     CDS             35554..36696
                     /locus_tag="IWO50_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ0357964.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            36709..37686
                     /locus_tag="IWO50_000825"
     CDS             36709..37686
                     /locus_tag="IWO50_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0357965.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            37686..38594
                     /locus_tag="IWO50_000826"
     CDS             37686..38594
                     /locus_tag="IWO50_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0357966.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            38595..39347
                     /locus_tag="IWO50_000827"
     CDS             38595..39347
                     /locus_tag="IWO50_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0357967.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            39352..40299
                     /locus_tag="IWO50_000828"
     CDS             39352..40299
                     /locus_tag="IWO50_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0357968.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        13470 a         6158 c         7882 g        12875 t
ORIGIN      
        1 ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa
       61 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt
      121 cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa
      181 cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt
      241 cgattggctt ataccagtcc tatccaacga aagatttatc gttcttaact ctagcggtat
      301 aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt
      361 agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac
      421 ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg
      481 ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa
      541 aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac
      601 catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga
      661 catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga
      721 tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg
      781 gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt
      841 tgctaagaca tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt
      901 aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta
      961 accatctact cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat
     1021 gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa
     1081 tccgataatg atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt
     1141 caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc
     1201 ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc
     1261 ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt
     1321 ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc
     1381 tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga
     1441 aacaacgctg atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga
     1501 ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg
     1561 gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat
     1621 caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc
     1681 tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc
     1741 agataacata ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca
     1801 tttaattatg attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt
     1861 taagtttcat gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta
     1921 attttgcata tcaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa
     1981 aaaattaagg agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt
     2041 ggcagctgct tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat
     2101 cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga
     2161 cttgagacaa gctctttttg agcagatcta tgatcattta caatatgaag tgttcactgt
     2221 tgagcataca gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag
     2281 agagaagaag ttgtatcgtt cactgtgctt agaagaatat ggtggaggaa aagagatgca
     2341 gaatttctct tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct
     2401 ttcagaagag cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc
     2461 agctttgatg tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca
     2521 agacagtata gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc
     2581 aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt
     2641 tcttacagcg tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa
     2701 gagccataca gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg
     2761 cgactagttc tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat
     2821 ccactttaac attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat
     2881 catccaccga tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa
     2941 tttttcccaa tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca
     3001 gatcgccatc atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat
     3061 ctgcatctac ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt
     3121 ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg
     3181 ctaaagccat gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa
     3241 catcattgaa aagaaaataa aacctaaaga cacctgggga atgtcatttg gctaaatgta
     3301 tgccgctatg ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa
     3361 aatcagcacg tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata
     3421 aaaatctgcg tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca
     3481 gaaactatat cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat
     3541 atgcgacttg tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt
     3601 tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg
     3661 attcttgttg ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag
     3721 taacgaagaa agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg
     3781 acatcggtca tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt
     3841 ttattggtca tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag
     3901 aagctggaaa agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta
     3961 ataccggaac ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg
     4021 gcggactttc ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt
     4081 tgacatcaca aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg
     4141 tacatgaaag tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg
     4201 actcaaaagc gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat
     4261 ttactcttgt ctttatttcc attgattatt gcttttggta atattttacc gtttctccac
     4321 atcgatcaag agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag
     4381 tttatcggtc ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt
     4441 tctgcattag cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg
     4501 aataaagcgt atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta
     4561 attgtcattg tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga
     4621 cagctgattt tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag
     4681 ttccaaactt tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt
     4741 tatttaattg tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc
     4801 gctactgtcg gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca
     4861 acgaggatca gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat
     4921 tttgctgcta cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg
     4981 acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa
     5041 actaaatttt ctcataaaaa aaagtagaaa caagagttct tattgtaaat ttatatcagt
     5101 aatttataat aattatgact cttaattaaa gtaggtgccc atatgcttgt ttcttttagt
     5161 atggttataa gattttttct gacaatttta ttatcactga tattaacacc tgtattcaaa
     5221 atcatttcag tacaaacagg tatggtcgac aaaccaaatg aaagaagaat aaataaagtt
     5281 ccaatgcctt cagcgggcgg actgcctatc tttatttcat ttgttatatc gactttattc
     5341 ttattccgta aaattatccc tcaatcgtat attgtaccga tcttgattgc ttctttagta
     5401 atcatagtca caggagtgct cgatgataaa tatgagctga cacctaaaca aaaaagtata
     5461 gggatcttga ttgcttctct tattatttat tttgtagcag atatacgtat tgattccttc
     5521 acgcttcctt ttattggata tatccaacta ggctggctta gttttcccgt aacgattttt
     5581 tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg gctggcggca
     5641 gggatttcat tgatcggttt aattacgata ggaatcattg gatatttttt cctccatgct
     5701 tctacagtgt atattccagt tgttattttc tgtcttgtag caagtattat cggttttttt
     5761 ccttacaatt tttatccggc aaagatttat ttaggcgata caggagctct attcctaggg
     5821 tttatgatgg ccgtcctttc actacaaggg ctgaagaacg tgacttttgt ttcatcgatt
     5881 tctttgctga tcgtcatggg agtgccggta acagacacgt tttttgctat cattaggaga
     5941 aaagcaaacc gtgtttcttt ttctacagca gataaaaaac atcttcatca ccgactaatc
     6001 agtttaggct ttactcataa aggtgctgtc ctgacgattt atgcgatggc tttgatgttt
     6061 tctttcacag ctatggtcat gaattatact ggaagaatcg gaacgatcat attgattatt
     6121 gccatgttgt ttgctgcaat tttattattt gaattgattg gtctgatcaa tgagaaacat
     6181 cagtttattt taaaaacatt gcgattttta gggaataaag agtaccgtac gcaagtaaaa
     6241 cagaaatatt tgaagaaatg gaaccgtcat atagatgaat aacaaattaa accagccgat
     6301 ccttattagt attgtgacgg caaacagtga taaaattttt cggacactag ataattttat
     6361 ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca attcacttcc
     6421 cgagtacaaa aaaagactaa cagagtatag gaaatatccc tacatcgata tctattttca
     6481 tgatgaaaat tccggatttg gttatggaca taatcacaat ctgatagcta gcgattctcg
     6541 ctatggcatt atttgtaacc cagatatttt agtggatgaa gagacagtta cagcattggt
     6601 cttgttattg aaagaacatc cggaatgtgc gatgttagca cctaagattc ttaatgaaga
     6661 tggaacaact cagcacctca tccgtgagca gctggctgtt tttgattacg ttttgcgctt
     6721 tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt ttgaatgccg
     6781 aggattgcct actgatgcta atagttatgt acggatgatc tcaggtagtt ttatggtagc
     6841 tgatattgat aaatttaaag agataaaggg gtttgatgag cgtttcttca tgtactttga
     6901 agacaacgac ttgtgtttga gttttgaaaa agcaggttac aagttattgt acacaccctt
     6961 ttatgcagtt atccattttt acgggaaggg tgcgcatcga aatgcaaaac tgtttcgcgt
     7021 gtttttgact tctatgatga aatttttcaa taagtggggg tggcgttttt tctgatgaaa
     7081 ccagaaatat tagtagtcgt tgtgctttat cagcaactat tcagtcaatc tccagcttat
     7141 ggaccattaa gcaaagcact tagcgaaaag caaattcagc ttattattta cgataatagt
     7201 cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata tcgtttatca tcatgatccg
     7261 caaaatcctg gtttagcggt ggcttataat tttgcgttgt ctcaagcaga tgaaggaaca
     7321 cgttgtctcg tcactctgga tcaggatacg aggctagtcg acgattattt tgaactattg
     7381 cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc cgatgatttt ttctgggagc
     7441 cgacaagttt ctcccctata ttcagatcgt tatatcaatc gtacagctca agcagtagaa
     7501 gttgaaggcc caacagctga acgaatcatg gcaatcaatt caggtgttgc ctggtctgtc
     7561 aaatttctaa aaaagattgg cgggtttaat cctgcttttt ctttagattt tttggatcat
     7621 tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg agatccttcc tgtacgaatg
     7681 gaacatgatc tttcggtttt agactatgaa aaagtttcaa gtaatcgata ccgttctatt
     7741 ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc cagtcaccga
     7801 cgacaattat tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg gcatatctgg
     7861 cggatgactt tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc gttcggtttg
     7921 tatagctacc tttaatggag gcagatattt aagagagcag ttagatagta ttttatctca
     7981 gctttcagca gaagatgaag tagtcatttc tgatgatggg tcagtagatg atacatggga
     8041 tatattaact gaatacgcag aaaaagataa gcgaatccag cttttttcgg ggcctcgtca
     8101 aggattgatc gctaattttg gttatgcgat ccagcagacg aaaggagatc taatcttttt
     8161 ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa aagatcactg actattttga
     8221 gcagaatccg gatcagctag tagtagtttc tgatttaatc attgtggaca gcgagctgca
     8281 agagatccat gattcgtatt tttcgtttcg aaattccaaa gaaggatggc ttaataatgt
     8341 gataagaagt cactatattg gtgctggtat ggctttccgg tcttctttaa aacccgatat
     8401 tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg attggtttgc tgggaggaaa
     8461 gaagactggg ttcctaagag aaccgttaac tctgtaccga agacatgatt taaacgcgag
     8521 tgagattgaa acacattcta aattacggca aaaaatttct tggcgtgttc atctgattac
     8581 ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat ccttaatatt
     8641 tccatgacaa atataacaat tgatgagata ataacaaagt gaaatttgaa atcaaaaagg
     8701 agagcagata tccatgaagg gaatcattct tgctgggggc agcggaacac gtctgtaccc
     8761 tttaacaaag gcaacatcga aacaattgat gccgatttat gacaaaccaa tgatttatta
     8821 tccaatgtct actctgatgt tggcgggaat caaagaaatt ctgattattt caacgccaca
     8881 agatacacca cgtttcaagg aattatttgg aaacggaaat gatttaggac tgcagatcga
     8941 atatgcagta caagaaagtc cagatggatt ggcacaagcg tttatcattg gagaagaatt
     9001 tattggagat gatagtgtct gcttggtatt aggggacaat atttactatg gtggcggtct
     9061 gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc gcgacagtat ttggctatca
     9121 tgtcaatgat ccagaacgct ttggtgtagt agaatttgat gagaatatgc aagctttgtc
     9181 gattgaagaa aagcctgctc agccaaaatc aaattacgct gtaactggcc tttattttta
     9241 tgacaatgag gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg gagaactaga
     9301 gattacggat gtcaataaag catatttgga taaaggaaaa ctatccgtag aacttatggg
     9361 acgcggattt gcttggcttg atacaggaac acatgaatca ttgcttgaag catctacctt
     9421 tatcgaaaca attgaaaaac gtcagaactt aaaagtggct tgtttggaag aaattgctta
     9481 ccgtatgggc tacatcgata aggaacaatt gctggccctt gcacagccat tgaaaaaaaa
     9541 tcaatacggt caatatttat tgaatttggc cgctgaataa ggagtcctta cagatgaaag
     9601 taacagatac aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt ggcgatcatc
     9661 gcggtttctt cacagaaagt tattcaaaag caaaatttgc agaacatgga ttgaaatatg
     9721 actttgttca agacaaccat tcactatcag ctgaagctgg tgtccttcgg gggcttcatt
     9781 tccaaaaagg agaagctgca cagacaaaat tgattcgggt agtcactgga gcagttttag
     9841 atgtagtagt ggatatccgc aaaggtagcc cgacttatgg tcaatgggac ggatttatct
     9901 tatctgaaca taatcatcga cagttgcttg tacctaaagg atatgcccat ggattcgtta
     9961 ctttgacacc aaatgtgaac ttcctttata agtgtgataa ttactataat gcagaagcag
    10021 atgggggcat cgcatttgat gatccagatt tagcgattga ttggccaatt gatccatcaa
    10081 gagcgattat gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt gaagcagaaa
    10141 atccatttgt ttacggagaa atctaagaat taaggagaaa ttttcttatg aaaaacatca
    10201 ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt tcattacgtg gtaaacaatc
    10261 atccagaagt gcatgtcact gttttggata aactgactta tgcaggaaac aaagagaact
    10321 tggccggact tcctagcgat cgcgtagaat tggtcgtagg agatgttgca gatgcagaat
    10381 tagtggaccg tctagtaaaa gaagcggacg cagttgttca ttacgcggca gaatcccata
    10441 acgataattc attaaaagat cctttcccat ttgtacaaac gaatatcatt ggtacgtata
    10501 cacttttaga agcttgccgc aagtatgatg tacgctatca tcacgtatct actgacgagg
    10561 tttacggcga tcttccatta cgtgaagacc tgccaggaca tggggaaggc gaaggagaaa
    10621 aattcactgc ggaaacacca tataatcctt caagtccata ttcttctaca aaagctggtt
    10681 cagatctgtt agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc atttcaaact
    10741 gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt tattcctcgc caagtaacga
    10801 gtatcttaag cggtattcgt ccaaaattat atggcgaagg aaagaatgtt cgtgactgga
    10861 tccatacgaa tgatcattct tcagcagtgt ggctgatttt gacgaaaggc agaattggcg
    10921 aaacctatct gatcggtgca gacggtgaag aagataataa gaccgtgatg gaattgatct
    10981 tagaaatgat gggccaacca aaagacgcat acgaccatgt aaacgaccgc gcagggcacg
    11041 atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga gctaggttgg cagcctgagt
    11101 ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa catgaagact
    11161 ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc caaaaatgga caataataca
    11221 gaggaaattt cactgtatta atggaaaaac acatgtgagg caggcgctcg cctgtctctt
    11281 ttttaaaata aaaatcaagg agcggataac catatgtttt tattaactgg aggaaatgga
    11341 caattaggta cagaattgcg ccatttatta gatgaaaaaa atctgccata tgtttcaaca
    11401 gatgcaaaag acttggatat cacagatgct gagaaaacaa tggcttatat tacagaatta
    11461 aaacccgatg ttattttcca ttgcgcagct tacacagcag tagataaagc agaagaagaa
    11521 gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa gaaatgtagc acttgcagca
    11581 aaagctgtga atgcacaatt cgtctatatc agtacagact atgttttcga tggaaaaaac
    11641 aaaaatgtag aatacgaagt agacgaccaa acgaatccac taaatgaata tggtcgtacg
    11701 aaattattag gcgaaaaagc agtagaagag attttagaag actattatat catacgaact
    11761 tcttgggttt tcgggatcta tggacacaat ttcgtcttca cgatgcaacg cctagcggaa
    11821 acccgcgaca aactgactgt agtaaatgac cagtttgggc gaccaacttg gacacgtact
    11881 ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac catttggtat ttatcatttg
    11941 tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag aaatattgaa agatacagat
    12001 gtagaagttg cacctgttac gtcagaagaa tttccgcaaa aagcaacacg tccgcagtat
    12061 tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg tcataccaac ttggcaagaa
    12121 gcacttgcgc aaatgctgga agctgccaaa aaaatgaata agtaatttcc tttttatcaa
    12181 atagaatttg gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc
    12241 aaaagcgaat caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct
    12301 tattggcgtt ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg
    12361 tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg
    12421 acagaatcgg gaagtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca
    12481 agctgaagag gacttttcgt tggtgaacga ggattattca ttaaacggac aaaacatgat
    12541 cattgcgtat aattctcctg taaaggatat tacggttatc ggaaaaccat tcaactgggg
    12601 attctttttc ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt
    12661 catggtcttg cttggattcg aagtactgat gatattaacg aagaggaaaa aagctctatc
    12721 gctaatcggt gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca
    12781 tgtgggcgat ttgattttct ttactttggg actgatgatt gctttttatc attatttcta
    12841 caatcatgat aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat
    12901 tgggtttatt ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt
    12961 ttattttgtt ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt
    13021 agtgttgatt ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac
    13081 gtcaaaagac gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac
    13141 tggaggaaag tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt
    13201 tgaagatatc aactattcga ataactctga agtggccctg ttctatcact tttttccaac
    13261 tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact
    13321 gggacgtatt ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc
    13381 aaaatggcta gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac
    13441 ttttagtttt gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga
    13501 tgttattcct atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta
    13561 tgcgattacg cattcgaaaa tggataatta tttttcggat tttgaaacca tcgcagttgc
    13621 tgtgttggga acagtgattt tagcttttgc attatttggc tggagacgct tgttttacct
    13681 cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac
    13741 aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc
    13801 tggtaaagtt tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa
    13861 atccttcaat acagtacgct tttatccaga tatagctgca tggaagaaaa tcgatccgaa
    13921 agaagaatac gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga
    13981 agatacaaag ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga
    14041 cgcagaaaaa ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca
    14101 attatatgaa gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt
    14161 taaagtagaa tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct
    14221 agtcgtacag taataggcta gctgagaata atcctgaaat attagtagaa aggagagtga
    14281 agataattca agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt
    14341 tgttgctgac gctagtcttt tttgtttgga cagcaggggt aggttttcca gcaactttgt
    14401 ttatatataa caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa
    14461 atgtttataa agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca
    14521 ttttcttttc ttactttcgg gaattaagaa atgagtcttt atttggagac ttactgtttc
    14581 ctttgattat tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg
    14641 gtatttatct gattgtcttt acggtcttcc tcggaatcac gttatatcgt gtatttacag
    14701 agatccaagt accagaagga caaagcatct gggctccagg caattcgctt tctgatattt
    14761 ggatcaatac gaatacaatc ggaagttctt tgatgactct tgctttattg atcagtggat
    14821 ttgccagttc ttttgaacga tggtatgttc gaatcatcgg tgggatagca gtcgttgcag
    14881 cctttttagg tatttgggtc tgccaatcta gaggtgcatt agtcgcgttg attgtttttg
    14941 cgatattgga tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgcct
    15001 acacggttac gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag
    15061 tcaatttgtt taccgatcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa
    15121 aatctgaaca agttttacta ggaatgaaaa catttatctt ccagcgagga aaccaatttt
    15181 taggaaacca caatagttat aattctattt tgaatatcta tggattgata ggatttggga
    15241 ttgctgcttt gctgcttatc ctttttattg gtcgcttaac gttgaaagca gatttatcca
    15301 acgggcagat gacttttatc tgggcctttt ttgcggtgat gatgcagtca tttatggagg
    15361 atacattgac ttcataccca tgggttccga ttatttatat cctgttggct atggccgttc
    15421 atcgatatga tgaaccagaa gttgcggagg aattctatgt tatggaagag gcatatgacg
    15481 atatgactta tgatgataca tcttatgagg aacaaccaga tagcgctcat tcttccagga
    15541 tccaacgcta tcattgatag gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct
    15601 tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta gagaaaatct
    15661 tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa atatggtaaa
    15721 atggcacttg actgtaattc actgaaaatc gcttggagga acacaaatgt ttaaagattt
    15781 gtatttattt atgaaggaca tttatcagaa cagaaaatta ttgctacagt tttcgatcaa
    15841 tgacttcaaa tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc
    15901 gctatttact gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat
    15961 cgatgcaaaa tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt
    16021 ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa
    16081 agtcgtattc aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca
    16141 tttattcttc attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc
    16201 aatgtcttta cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac
    16261 ttggctgacg gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt
    16321 gctgcagttg atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg
    16381 tcttgtaagc atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc
    16441 atttttaagt gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt
    16501 cacgttagta atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc
    16561 agatgtgctg taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca
    16621 aaatcttata atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt
    16681 aagaaatcct atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa
    16741 ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc
    16801 attacgggag tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct
    16861 ttgctagaac taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta
    16921 aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt
    16981 gaatttgctg acatcggaga ccatatttat caacctgtaa aaacttattc aagcgggatg
    17041 ttcgttcgtc tagcttttgc tgtagcaatc aatgtgaatc ccgatatttt gattgtggat
    17101 gaagcattgg cagtaggcga tcttgaattt caactaaaat gtatggaaaa atttacagaa
    17161 atcaagaact cgggaaagac gattttattc gtttctcatg atatcagttc gattcgtcgt
    17221 ttttgtgatc gttcctattg gttgaaaaac ggagaagtcg tcgaatatgg cgatacgatg
    17281 gaagtaacga cgaattatga aaacttttta aagaaaaaat cagttaaaac agtggatcgg
    17341 aataaaaacc aagtagaacg gttcgccgct ccagatatcg tcgacgtgac gaaagcggaa
    17401 ttgttggata aagacaaaaa tccggtggat atgctggaac aaggagaaaa actatacgtt
    17461 aaagtaactt atgaagtaaa agatgataca atcaaaaatc cggttttagg aattgcgcta
    17521 cgaacagtcg acaattttta tgtatgcgga cttaatactc tactagatgg tatcaagatt
    17581 ccttggaaaa aaggagagaa tatcttttat ctggagtatg aaaagttagg tttgttagct
    17641 ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatat
    17701 aagacaaagt acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc
    17761 ttagaccata aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct
    17821 agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt
    17881 agagtttggt cccggaaacg gccgattaac gaaacattta atcggagcaa aacaatgtga
    17941 agtgagcatt gttgagttag ataaggaatt gttcgatttt gtcagcgaat ttgctcaaga
    18001 tggattttat ggtgatatcg aaagttttga gtgggcaaac tattatgctg gacagacatt
    18061 tgattacgtc ttgtttgcgg atgttttaga acatttggta gatcctggaa aaacattgaa
    18121 gaaagtcaga gaatttttga atgaagaagg agagatcctg attacattcc ctaacttggc
    18181 gcataattct gtcatgatcg atttgttcaa taaccaattg ccgtgggctt cttatggtct
    18241 cctagatgaa acccataatt ctttttatac tcatgatggc ttccaaaaag tttttgaaaa
    18301 agcgggatta ttcattaata ttgaagatta tttgtactta gctgtaggtg atacagaatt
    18361 aaaatcaact tatgaagaat tgccggaagc tgtacgctat gatttcaaaa tgcgaccatt
    18421 tggtgaagtc taccaatact ttttctcatt gatgaaacat ccagtagcac aaagttccat
    18481 tgcagagcca caaaattcta attatgtaaa agtgttagaa gtaacccagc aaacgaaaca
    18541 agatgaaaca atccaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag
    18601 tttccccgta tcagaaacaa cagaaagaat ggtgtttcgc ttcgcgcaac aacctagctt
    18661 tatcgaattc agcgcagaag ctgccggaga aaaactaaca tttatcgatt cgaatgctgt
    18721 agtcaaaaca gtaaatgatt gttatttgtt tgatgggaaa gaattaccag agttcgttct
    18781 aacggatatt tctgggaaag aagtgactat ccattgtcac tatcgcttta tcggggaatt
    18841 gacaccaaca atgaaagaat tgttggaaac gattcgtccc atggctgaag tcacaaagca
    18901 gctttctgag aaaaatgacg aattagaaag agaaaatcat catcttcttg aggaaaatac
    18961 aagactagac catacgttaa aaacaaccac aaatcgctat tgcacattgt tggaaagtga
    19021 tgaatggaca ataaaacata gactaggacg acgtaagaaa gaaacttcga aaaaaatcca
    19081 agaaaaagaa ctttctatat gtattgacga gaaaatctgg gatgcagaaa caaaaatatt
    19141 aaaaatcatc ggatggggaa tcgctaattc ggatcgccag cccttgtcct ataagttatc
    19201 agctgaccaa tcgccatttt tcgaagcaat tcctgtttca cgagaagaag tcaatcaagc
    19261 agaacagctc gcaaaaggaa cagaagcggg atttgagtta cgaatccttt gcgagcaaga
    19321 acgttcattc ttagtggaag cagtagctca aaatggccaa tcatggatca ttgaaatgta
    19381 aataatgatt aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc
    19441 gtatttatcg atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg
    19501 gcaattgatg aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt
    19561 gctgaatcaa cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag
    19621 acccaaagcg gtttcaaggt acgtttatca gggaaaatga gaggaaaagc catcttagac
    19681 ttccaaacag cgagacatca aaatggcata gctgtgaaat taaacggtaa atacccttat
    19741 gatgacggca ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat
    19801 tatgcaagga ctcatggcgt aaaaaaagcg atccgtcggg tcaaattgga attgaagcca
    19861 ggatcgatcg actatgcaga gtggatcagt cgtcatgaga aaccagattt gaaagagcaa
    19921 aggaaagaag cacaaggatt tgcgtaccgt ccattgattt ccatacttat gcctgtctat
    19981 aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg tactggatca aacgtatgat
    20041 cattgggaac tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtctg
    20101 gaatcctatc aagcaaaaga tgatagaatc aaagtcgttt tccgaaaaga aaatggacac
    20161 atcagtctgg caactaattc tgcattggaa atagcagaag gagaatttat agcactctta
    20221 gataatgatg atgaattacc gccatttgca ttatatgaag tggcaaaagt gctaaacgta
    20281 catccagaac tggatgtgat ctacagtgac gaagataaaa tcgatgcaga tggcaatcgc
    20341 tttgatccgc atttcaaagc agactggtcg cctgatacat tgatgggaaa taattatatc
    20401 tctcacttag gcgtatatcg aacaagcatt gttaaagaac ttggcggttt ccgtaaaggc
    20461 tatgaaggtt ctcaagatta tgatttggtc cttcgtgtga cagaacaaat ccctgcagat
    20521 catatttacc acattgaccg agtcttgtat cattggcgga cgatcccagg atcaactgca
    20581 agtaatggcg aagccaaaag ttatatctat gattccggag tgaaagcatt gacagatgcc
    20641 ctgtctagaa gaaacatcaa aggaagcgta cgtcctggca gaatttctgg attttatgag
    20701 attgcttatg acgtcttgca agaggatttg gtcagtgtga tcattccaac gaagaatggc
    20761 tatgaagatc taaaaacttg tgtggattcg atcatcgaaa aaacgtctta ttcaaattat
    20821 gaaatcatta ttgcggataa cggaagtaca gatccaaaaa tgcaagaatt atttgcagaa
    20881 tacaagcatc agctgaaaga ccgctttatc gtagaattga ttgacattcc attcaattat
    20941 tctcgaatta acaatttagc agcagaaaaa gcaaacggca aatacttttt attcttgaat
    21001 aacgatacag aagtgattga gccagattgg atgacggtga tggtttctta tgcacagttt
    21061 gatcgaatcg gctgtgtagg agcaaaactg ttttatccag atgatacaac gcagcatgca
    21121 ggagtattat taggaatcgg tggtgttgca gggcatgcat tgaataacta tgatcgaaca
    21181 cattgtggtt atttcggccg attggtcatt gacgtcaatt acttagcagt cactgcagct
    21241 tgtatgatgg tcaaggcagc tgacttcaat gctgttaacg ggtttgatga aacattagaa
    21301 gtagcattca acgatgttga cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc
    21361 tatgcacatc aagcagaatt gtaccatttc gaatctaaat caagaggtta tgaagatact
    21421 ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa tgcaagataa atggccagca
    21481 tatattgcgc atgatccatt ttacaatgat aatttaacaa aagacggaat cggcgacttt
    21541 tcgttgagac cagattaaac tataataaaa agctgttatg ttgtgattcg ctcgcacata
    21601 acggcttttt atagtttttt gtcaaaaaaa ggcagaaggt gccgactcaa aaaaataagt
    21661 cgaggttggg acagaagtgt ttaactccga gaaataagaa gaaattcacg aaaattgctt
    21721 ttcaaatttt tgtgaatttc ggcttattta agaagaagtt gcttctgctc ccaccgttta
    21781 ttcgttttta aagaatgaga caaaagtgtt tcttactttt gtcccacgct cttattcatg
    21841 ctcagagcgc caatagatac aaaatcatag ccaaagtttc acgaacccta gctgtcaaag
    21901 aatcgatatt ttattttaat ataggctatt ttaaaataaa tggtttaagg tattcctcta
    21961 caaaagccac tctatcaatt atcatgggtt taaaataaga agtgatggag ataaccagat
    22021 tgttcttagc gtcaatataa agaacatttc cactattacc aattgctgaa tatatttcgt
    22081 tctcagaatc taatacccac cataaatagc catatctcat attttgtaag tcttctaaag
    22141 cactggttct aggtttgaac atctcgttta tccacatggg tggtacgatt tgttttcctt
    22201 tatacatccc tttatttagg cataatacac caatttttgc cagatcactt gccgataaac
    22261 acaaaccata tcctgccgtc ccgatattta gtggatcaca aaaccagaca ttggttttag
    22321 gagacttatc cattgtaaac tgcttatgtt cttcagctgt ttcagcataa taattgacat
    22381 ggtttggtat ccctatagga tcaaatagat acttattggc aaaatcaaca gttgttagct
    22441 tacttgtctg atacaacaag ccagttaata tgtgtaaaca gacagtttga tagttgaatt
    22501 cgccggttat accttttctt cctcccaata aatctagtga tgtttttgtc caatcttcac
    22561 ttgaacagac ttttgtccat ggatcgcctt tacatttgta gggtgctttc attgttagca
    22621 agtgcttgat tgtaatagcg taaatagttt tctccccgcg tttcacttta tactctggaa
    22681 aataatctaa tactttatca tcgatacttt tgattaatcc ttgctctaaa gcaatgccaa
    22741 tcagcaaaga tacaatactt ttcgttgctg acatgatatg acaagtatcg tcttttttat
    22801 aaccattcca ttcatctgta taaacttcta ccccatcctt atatgcgaca atttgacata
    22861 tatttggttg tcgaaaacca ataaaatcat gcaactcttg tttattcata agatattacc
    22921 tcactttctt ctataagcat aatgtcatta aaaatcgatt tcaatttttt cgattcttca
    22981 cgtaatacag gtattcttat ggactagaca gcgctttggg aagatggatt ctctgaaaaa
    23041 tataaaggtt cactttatga gtggataaaa aaagcagcaa atacttcgac ttctttaatt
    23101 taccaacatt agtgaaaatc gattcatctg gagatttgta atttagagtc cttgtcttga
    23161 ttagaggtgt ataaagcttt ttattagaca tttttaaatt ccattacaat ttgcttataa
    23221 actaggtttt taaaggtgac tatggtaaga taatagagat attattttgt gattaggagt
    23281 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
    23341 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
    23401 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
    23461 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
    23521 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
    23581 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
    23641 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
    23701 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
    23761 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
    23821 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
    23881 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
    23941 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
    24001 attgaagcat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
    24061 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
    24121 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
    24181 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
    24241 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
    24301 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
    24361 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
    24421 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
    24481 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
    24541 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
    24601 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
    24661 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
    24721 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
    24781 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
    24841 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
    24901 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
    24961 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
    25021 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
    25081 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
    25141 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
    25201 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
    25261 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
    25321 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
    25381 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
    25441 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
    25501 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
    25561 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
    25621 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
    25681 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
    25741 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
    25801 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
    25861 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
    25921 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
    25981 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
    26041 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
    26101 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
    26161 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
    26221 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
    26281 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
    26341 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
    26401 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
    26461 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
    26521 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
    26581 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
    26641 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
    26701 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
    26761 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
    26821 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
    26881 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
    26941 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
    27001 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
    27061 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
    27121 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
    27181 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
    27241 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
    27301 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
    27361 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
    27421 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
    27481 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
    27541 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
    27601 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
    27661 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
    27721 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
    27781 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
    27841 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
    27901 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
    27961 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
    28021 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
    28081 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
    28141 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
    28201 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
    28261 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
    28321 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
    28381 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
    28441 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
    28501 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
    28561 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
    28621 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
    28681 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
    28741 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
    28801 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
    28861 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
    28921 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
    28981 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
    29041 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
    29101 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
    29161 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
    29221 atttcttact tgtatttaaa aaaggagaat taatatgaag acagctttca ttgcttggac
    29281 tcctttacat ttgattaata tattaaatgt ttatcaaaat ttttaccgag aatcaaaagg
    29341 agatttattt atttattctg aatttgaaaa cgctagaaga tatagtgagc gtataaaaga
    29401 attagggcta tttaatgatg ttgtttttgt gaatcaccgg aaaatcggtt cttcctttaa
    29461 acgtaaaatt aacttgttaa ctaaccataa tcaatttttc tcggatgatt tatccgttta
    29521 tgatgaaatt tttatacagg gggaaaatta tttcacaaaa atacttttct ccaatgttta
    29581 caaaaaaaat ccagatgtaa aactaaatta tatagaagat ggtttgggta cttatttgaa
    29641 gaagagtata ttttcaacaa gtaaaaacgt tcaaagagtt tttaaaatat taaataaaaa
    29701 tagcatcttc aaaaaaaaaa ttgacgatta ttttgtctat tgtccggaat tgttacttta
    29761 tgatgtaaaa aatataaaaa aaattcccaa aatagatgaa aacagtaaat tgatgaaaaa
    29821 catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta tcttttttga
    29881 tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg ctaaagtaaa
    29941 tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa
    30001 tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga
    30061 aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc
    30121 tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt taaatgaata
    30181 tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt tctgtgaaca
    30241 ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag aattaagaaa
    30301 gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt aagtccagtt
    30361 tctggtatac tatagggaat ttttttatta agggaatttc attcataact attcctattt
    30421 ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact tcatttgtat
    30481 caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat gcaagttttg
    30541 actataaaga tgacataaat gaatttatgt catcggtact ttctctagct acactttcgt
    30601 taattgtgtt cttattaata gggaacatat attttttgtt ttcaaatgct ttttttgata
    30661 tgaaccaact tgtatttaat ttgatgttat tacaaagttt tgctagtttt gtaattagtt
    30721 atatccagtc ttattttacg attaacttta accattttaa atatttgatt ttatctttta
    30781 tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta tattttgatg
    30841 ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa
    30901 tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag tggagatacg
    30961 ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt ttagcacaat
    31021 tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata tattcctaca
    31081 ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac gcctgggttc
    31141 catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact
    31201 attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg attgatttag
    31261 caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt ccgataacct
    31321 taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt tatgagaaaa
    31381 aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata ctattaaaca
    31441 tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta tttacgtatt
    31501 ttttactttt tatttttcat tataatatcg ctaaaaaaat tacgaataaa caattatttg
    31561 acacaaaaaa aatatgtatc ataataattt ttgtatcaat tttttctttg atacttcata
    31621 ttttaatcaa tcaaatgttg attcgttacg ttttgacatc attattgata attagtttag
    31681 ttatatattt tttaaagatt aagaatcgaa ttatagatga ctataattca taggaggaga
    31741 tataagtgtt taaaggaaaa acaatattag taactggcgg aactggttcc attggaagtg
    31801 aaattgttcg tcagctttta aaatatgaac caaaagctat cagaatttat agtagaggtg
    31861 aagaaaaaca attttatatg caacaagagc tctcaaatca cactaatttg agatttttaa
    31921 ttggtgatat cagagactct caaaggattg attatgcatg tcgtgatgtg gatattattt
    31981 ttcatgctgc cgcaatgaaa cacgttccag caagtgaata taatcctatg gaagctgtaa
    32041 aaacaaatgt gatgggaact caaaatgtaa tagatgcagc gatcaaaaat aatgtaaaac
    32101 attttgtagg tatcagtaca gataaagtag tgagtcctac taacaccatg ggtgcgacaa
    32161 agttattgag tgaaaaatta ataatagctg ccaatcagta taaaggaaaa acagaaacaa
    32221 tattttcttg tgtaaggttt ggaaatgtca tggggtcaag tggatctgtt attcctcttt
    32281 ttagtaaaca aataaaaaac aataaagata ttactttgac agatgttgat atgacacgat
    32341 ttatgatgtc aataccggat gctgcaagat tagttctgtc tgctgctatg aattcaaaat
    32401 ttggagaaac atatgtctta aaaatgccgg caatcaatat tttaacttta gctcaatcat
    32461 taataaaaat atataatgat acacatgatg aaaaatatag aggtgaaata aaagttgtag
    32521 gtatcaggcc aggagaaaaa ttatatgaag agctaatgac tacagacgaa attgaacgct
    32581 catatgaaaa tgataaaatg tatatcattc caccacacta tcatgctact gacgaactgt
    32641 acaaagatta tgaaaaaatt aaaatcactg catactcttc agatgcaaca gaattaatga
    32701 gtggacagga atttattaga atcgcaaaag agaataatgt cgtgacttgg taaggagttt
    32761 tatgatgaaa tcagaagtat atgtaattgc agaaattggt gtaaaccata atggaagtct
    32821 agatttagct ttagaaagta ttgataaagc taaagaatgt ggagctaacg cggttaaatt
    32881 tcaaacattt aaaacgaata agctagtttc caaaaaggct ccaatggcaa aatatcagaa
    32941 ggataatctt aaagaggaca gttctcaatt tgaaatgcta aaaaaattag aattatctca
    33001 atctgatttt attagaataa aagattattg tatacaaaat aaaattgatt ttttgtctac
    33061 tccttttgat gaagaaagtg cagactttct aaaacaaatt ggtgtacaag gatttaaaat
    33121 tggctcagga gatttaacaa atttaccttt tcttagaaaa atagatatgt atgggttgcc
    33181 agttttatta tctacaggta tggctgatat tcatgaggtc agtgaggctg tagattgttt
    33241 catagatagt ccagttacta ttttacattg tacttcaaat tatccggcta gttctgaaga
    33301 tattaattta ttggcgatta acacattaaa agaaatgttc gggatacccg ttgggtactc
    33361 cgatcattca ttgggatatg atgttggtat ttgtgctgtt gcgatgggtg ctaaggtaat
    33421 tgagaaacat tttaccttgg ataaggaact acctggtcca gatcataaag cttctttaga
    33481 tcctcaggaa tttgcagatt ttgtaaagca tattagaaac actgaaatat ttttaggtga
    33541 tggtgttaag cgagcaatgc cttctgaact tagtacaaaa gaagtatcaa gaaaaagcct
    33601 agtggtcact gagaatttaa atgtgggaga tattcttaca gagaaatctc ttacaataaa
    33661 acgtcctgga aatggaatag ctcctaagtt tttatatcaa tatattggca aaaaagttaa
    33721 aagaccaata agtgcagata gtgttctgct tgaggaagat gttgaatgaa aaaaatactt
    33781 atagtaagtg gaacacgagc tgattatgga atatataaac ctgttgctga gaatttacta
    33841 caagataatt gggatgtatc atttgttgtt tctggaatgc atttatccca taaatatgga
    33901 tttactaaag aattgatctt agctgacgga tttagaatta gtggagaagt tcaaagtttg
    33961 tttttagaga acaataaggg aaacatggct cggtctgtgg cattagagat aaatggtttc
    34021 acaaatatat ttgaacaaac tagccctgat tttgtattat tactagggga tagaggagaa
    34081 atgttggcag ctgcgatagc ttgtttgtat cttggaatca aaacagcaca tattcacgga
    34141 ggcgaagtgt ctggtactgt agatgaatca ataagacatg ccataacgaa aatagcatct
    34201 gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa taaaaatggg ggaagattct
    34261 tggagagttt ttcccgtcgg agctcctaga atagatacta ttttaaataa tagattacct
    34321 agttttagag atataaaaga aaaatatcat ctaaaatttg aaaagaaaaa atacgttttg
    34381 caagtatttc atccagttgt aaccgaacta gaaaatatag aaaaacaaat tttagctttg
    34441 atcaattcaa tggaacaaca atctcaacca attatttgca ttttacctaa tagtgatgct
    34501 ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa aaacggatat tatttatatt
    34561 acgaatttag agccggagga atacctgact atattaaaag agtgcaaatt tatgatcgga
    34621 aattcttctt ctgggataat tgaagctgca tcattcaaaa taccagtaat aaatttagga
    34681 accagacaaa atggacgaga acaatctaaa aatattataa atgcaaatct aaatgaaaat
    34741 gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt ttataaaaag cttgaaaaat
    34801 gttaagaaca tctacggtga cggcaacagt tctagaagaa taacaaaaac attgaatcaa
    34861 atattgaatg atggggaaga tattaagtgg attcaaaaac aaatagctta ttaaaatata
    34921 ctagtgatcc atacattatt attggggctg gcggacacgc aaaagtcatt attgaaacct
    34981 taagattaga aaataagtta atatatggat tttttgatga taagcaaaat aaaatgcaag
    35041 atctgaaata tcttgggaaa ataaacgaat tgaatagctg ggcggatagc aaaaaaaaaa
    35101 tctataaatt tattgtagct attggtgact gtgtagtaag aaaaaagatt ataaaaaaat
    35161 tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc aagtgctata gtggcaaaag
    35221 aaatttcaat tggttatgga actgttattt ttgcgaatgc tgtaattaac ccagatgctg
    35281 taattggaga gcatgctata ataaacacag gtagcattgt tgagcatgat tgtagaatta
    35341 ataactatgt tcatttatct ccaggtgtat gcttagcggg tggagtacat gtaggtgtag
    35401 gaactcaaat aggaattggt tctcaatgta tccagatgaa gaaaattgga tcttggtgta
    35461 tgataggcgc agggagcact attgttaagg atattccctc acattcctta gcttatggca
    35521 atccagctaa aattaagaaa gaaggaatta attttgaatt ctagaatttt attatcaagt
    35581 cctcatatgg gaggaacaga agaaaaatat gttgctaagg cttttaaaga taattggata
    35641 gcgccattag gtgaaaatgt ggataaattt gaagaaagta ttacaaaata tactggttct
    35701 aagggcggag cttctgcgtt aagttcaggt acagctgcca tacatcttgc cttaagactg
    35761 cttaatgttg gaaaaaacga tattgtcttt gctccttcat ttacttttat agctacagta
    35821 aatcctattt tatatcaagg ggctgtacca gtgttcattg actcagaacc taatacatgg
    35881 aatatgagcc ctgttgcact agagagcgca ttagaagaag ctgaaagaaa caaccaatta
    35941 cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg ctgattatga acgaatttta
    36001 gctattgcaa accaatttaa tgttccagtg atagaagatg cagcggaatc gttaggttct
    36061 acttataagg gaatttcgtc aggcttattt gggacaattg ggatctattc atttaacggt
    36121 aataagatca tcacaacctc aggtggagga gctctagtgt ctagtgataa aaaatatgtt
    36181 gagcattcaa gatttttagc aacacaagca agagatcaag caccgtatta tcaacatagc
    36241 gaaataggtt ataattaccg tctaagcaat atttctgccg gtatcggacg tggacaaatg
    36301 gaagtattga atgatagagt agctaaacga agagaaattt tcaatcgata taaggaagct
    36361 ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg atggcatggt gccaaatcgt
    36421 tggttgtcta ctttaacgat caatgattat aattctattg atcaagtagt taacgtaata
    36481 aataaactaa atgaaaaaaa cattgaaaca agagctttgt ggaaaccact acatttgcaa
    36541 ccagtatttc aaggggtaaa aatgtattac catgataata atcttagtgt gtcagaaaag
    36601 ttatttaata gcggcttatg cttaccttct ggaagtaata tgacagatag tgaacaagaa
    36661 tttgttattg atgagtttaa aaaaatattc aagtaggtgt gatattttat gaacatagga
    36721 attattggtt taggttccat ggggaaaaga agacttagac tcattcaaga acactttaat
    36781 gatattttac tatgtggtgt tgatactaat gaggcgagac gaaaggaagt tgaaaaggaa
    36841 tttggaatac caacttataa tagtctaaca gaggcaaaaa atttgtttga aataaattca
    36901 gtttttgttt gtacatcccc tataacccat gaatttatta taaaagaggc tttaaattta
    36961 gattgtaata tctttacgga aatcaattta ttaaatgaat attatgatga aataatcgaa
    37021 gtagctaaaa agaaagatag attactttat ctatcttcta catttttgaa gagaaaagaa
    37081 attcaatata ttaagtctaa agtgacgcat aataaaaaaa tttcttatca ataccatgta
    37141 ggacagtatt tacccgactg gcatccttgg gaagattatc gcaatttttt tgtttcaaac
    37201 aaaaaaacta atgggtgtag agaaatattc gctattgaat taccttggat tatagataca
    37261 tttgggaaag ttaaatcgtt tactgtaatg acccagaaat tatccaatct tgatattgat
    37321 tattcggaca gtttcactct tattattgag catgaaactg gtatagttgg aactttaagt
    37381 gtagatgttg tttcaagagt agctaaaaga aatttaacta taattggtga aaagatttta
    37441 atagaatggg aaggtaaccc ggattctcta ttttcatgga atgaagaaac ttctagtatg
    37501 acagatattc aattgtacga tgaggttatg catgataata attattcaca tactattatt
    37561 gaagatgcat atttagagga gattaaagaa tttttcacgc ttttggaaaa gaaaatggaa
    37621 ccccaatata cttttgaaaa agataagtat attattaact taattaacga cattgaggta
    37681 aattaatgat gaaagtaatt ttttttgggt tagggtcaat tggaaagaga catctaaaaa
    37741 atctaataag ctatggaaaa aagaattcta ttaattttga tataacagct tatagtagca
    37801 gtggaaaaaa agagtttgaa gaaataacgt atattcaaaa tagagacgat ttggatacac
    37861 attatgatat agcttttatt acaaatccga cttttaagca tctggaaaca ctaaatgtaa
    37921 ttgttgaaaa agttgattac atatttttag aaaaacctgc tttttctgag agtattgatc
    37981 ttactcaata taattatgat tacaaaaata tttatgttgc tgctccactg aggtataaaa
    38041 atatcatgga agttgttcgt aatagtattc aaagtaaaaa agtatattct gtaagagcta
    38101 tttgttctac ttatcttcct gattggagaa aaggtactga ttatagaaat aattattctg
    38161 cctttaaatc tatgggggga ggagtagaat tagactgtat acatgaattg gattatatta
    38221 taaacctatt tggtttccct aaatcagtaa aaaaacagtt tggtaaatat tcgaatcttg
    38281 aaattaacag taatgattta gcaacatata tattgagata cgatgataaa attatagaaa
    38341 ttcatttaga ttattttgga agagttccta caagaaaatt tcaattgttt actttagatg
    38401 aatgtatcaa tgttgactta ttaaataata aaataagttt tgaagaaaac attgttaaag
    38461 aaatgcctga atctgctaat gatatgtatg agaaggagtt gaaatatttc tttgaaaacg
    38521 tagtaacagg caaagaaaac tggaatgatt tatatcatgc aaacgaagta ttgaaaatag
    38581 ctgaggagaa ataaatggaa attttattta ctatttgtgg tagagctggg tctaaggggt
    38641 ttaaaaataa aaatttgaaa tatttggcag gaaagcccct agtccatttc gctttagcaa
    38701 tgatagatat ctatcaacaa aaacatcctg aacataattg cacgacttgt ttgaatacgg
    38761 acagtaatga gttagtagaa ttgacgacta agttaaatcc gtctgtgaaa attgtagaac
    38821 gtaaacctga attagctaca gatactgtag ggaaaattga agtgatacgt gattcattct
    38881 tacaactaga aagagaagat aatatatttg atttaattat agatttagat ttgacttcac
    38941 ctttacgtga agtttctaat cttgaagaat taatagatga acatctaaaa aatttagata
    39001 aagacgtcta cttttcagtt gtttctgcaa gacgtaatcc ctattttaat atggtaaaaa
    39061 gaattgaaaa tgaagttact atagttaata aatcaagata cacatctagg caacagacac
    39121 ctgaagtttt tgaaatgaat gcctcaatgt attcttttaa acctgaattt ctaaaaaaaa
    39181 gtaattatat ttttgatggg caatgtggga taataaaaat gagagattat ctaattttag
    39241 atattgatag tgaagaagat tttaaatggt tagaattttt gtttcctaaa tttttagaag
    39301 attcttcgga gcttcaagat atctataatt atttatatcg gaggtaaaag gatgagctgt
    39361 tatcttatca ccggtggggc tggcttcatt ggttctacct tagccaatca tttaggagaa
    39421 gaacatgtgg taatcgtcgt cgatgactta tcaatgggaa aagtagaaaa tcttgatatg
    39481 acgaaaaata tcacttttat tgagggagat gtcgctgaga aatccttgat ggaagagatc
    39541 atgaaagcta ataaatttga ttatatcttt catttagcag cagttgctag tgtggcagat
    39601 tcagttgccc gaccagtaga aacacatcgt gtgaattttg aaagtgtatt aatgatttta
    39661 gaattgatcc gcaagtatca acctgattta aaacggatcg tgtttagttc ttcagcggct
    39721 gtttatgggg atgaaccgac tttaccaaaa aaagaagagt ccgtgattcg cccattgacg
    39781 ccttatgcgg ttgataaatt cgctgcagaa caatatgtgc tagattattg tcatttatat
    39841 gatgtacccg gtagcgcagt tcgttttttc aatgtctatg gtccaaatca aaatccgaat
    39901 tctccataca gtggtgtgat ttctattctc gtagatcgct ataagaaaca aatcgtagga
    39961 gaagcaactt catttacatt atttggtgat ggaagccagt ctagagactt tgtttatata
    40021 gatgatgtaa tccaagcatt acttttagtt gcgaatgagg aagctgcact ggggaaacaa
    40081 ttcaatgttg gaacgggtaa agcaaccaca cttttggaat tgattcatgc aatcaatgaa
    40141 attctcggtg tagaattgac acttgaatac aaagaagagc gttcgggtga tatccatgac
    40201 tctctagcag atattactaa gatccagtca attggctatc aaccgaaata tggtatttta
    40261 agtggaatga ctaattattt gaaaactgaa atcaattaaa acatactaag ataagattag
    40321 agggtaaatt caggctcttt gtcaataagg actgatgagt tttgtaaaat caaatctggg
    40381 ataga
//
LOCUS       DACEDM010000017        40121 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEDM010000017 DACEDM010000000
VERSION     DACEDM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.017"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(113..694)
                     /locus_tag="IWO50_000829"
     CDS             complement(113..694)
                     /locus_tag="IWO50_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0357969.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(877..1503)
                     /locus_tag="IWO50_000830"
     CDS             complement(877..1503)
                     /locus_tag="IWO50_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0357970.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1525..2856)
                     /locus_tag="IWO50_000831"
     CDS             complement(1525..2856)
                     /locus_tag="IWO50_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0357971.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3011..3412)
                     /locus_tag="IWO50_000832"
     CDS             complement(3011..3412)
                     /locus_tag="IWO50_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0357972.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3498..3704)
                     /locus_tag="IWO50_000833"
     CDS             complement(3498..3704)
                     /locus_tag="IWO50_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357973.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3799..5301)
                     /locus_tag="IWO50_000834"
     CDS             complement(3799..5301)
                     /locus_tag="IWO50_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0357974.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5319..5774)
                     /gene="argR"
                     /locus_tag="IWO50_000835"
     CDS             complement(5319..5774)
                     /gene="argR"
                     /locus_tag="IWO50_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0357975.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5872..6489)
                     /locus_tag="IWO50_000836"
     CDS             complement(5872..6489)
                     /locus_tag="IWO50_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357976.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6710..8083
                     /locus_tag="IWO50_000837"
     CDS             6710..8083
                     /locus_tag="IWO50_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0357977.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8158..9270
                     /locus_tag="IWO50_000838"
     CDS             8158..9270
                     /locus_tag="IWO50_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0357978.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9342..10979)
                     /locus_tag="IWO50_000839"
     CDS             complement(9342..10979)
                     /locus_tag="IWO50_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0357979.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(11147..11755)
                     /locus_tag="IWO50_000840"
     CDS             complement(11147..11755)
                     /locus_tag="IWO50_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0357980.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12139..13290)
                     /gene="queG"
                     /locus_tag="IWO50_000841"
     CDS             complement(12139..13290)
                     /gene="queG"
                     /locus_tag="IWO50_000841"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0357981.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13309..14061)
                     /locus_tag="IWO50_000842"
     CDS             complement(13309..14061)
                     /locus_tag="IWO50_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357982.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14202..15152)
                     /locus_tag="IWO50_000843"
     CDS             complement(14202..15152)
                     /locus_tag="IWO50_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0357983.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15153..15992)
                     /locus_tag="IWO50_000844"
     CDS             complement(15153..15992)
                     /locus_tag="IWO50_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0357984.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15989..16735)
                     /locus_tag="IWO50_000845"
     CDS             complement(15989..16735)
                     /locus_tag="IWO50_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0357985.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16973..18343
                     /locus_tag="IWO50_000846"
     CDS             16973..18343
                     /locus_tag="IWO50_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0357986.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18398..19294)
                     /locus_tag="IWO50_000847"
     CDS             complement(18398..19294)
                     /locus_tag="IWO50_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0357987.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19575..20252)
                     /locus_tag="IWO50_000848"
     CDS             complement(19575..20252)
                     /locus_tag="IWO50_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0357988.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20343..21248)
                     /locus_tag="IWO50_000849"
     CDS             complement(20343..21248)
                     /locus_tag="IWO50_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0357989.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21423..22877
                     /locus_tag="IWO50_000850"
     CDS             21423..22877
                     /locus_tag="IWO50_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0357990.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            22893..23924
                     /locus_tag="IWO50_000851"
     CDS             22893..23924
                     /locus_tag="IWO50_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0357991.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23931..25118
                     /locus_tag="IWO50_000852"
     CDS             23931..25118
                     /locus_tag="IWO50_000852"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0357992.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25274..26656
                     /locus_tag="IWO50_000853"
     CDS             25274..26656
                     /locus_tag="IWO50_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0357993.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26628..27014
                     /locus_tag="IWO50_000854"
     CDS             26628..27014
                     /locus_tag="IWO50_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357994.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27214..27882)
                     /locus_tag="IWO50_000855"
     CDS             complement(27214..27882)
                     /locus_tag="IWO50_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0357995.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(28071..28868)
                     /locus_tag="IWO50_000856"
                     /pseudo
     CDS             complement(28071..28868)
                     /locus_tag="IWO50_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(29149..30528)
                     /locus_tag="IWO50_000857"
     CDS             complement(29149..30528)
                     /locus_tag="IWO50_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0357996.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(30703..31872)
                     /locus_tag="IWO50_000858"
     CDS             complement(30703..31872)
                     /locus_tag="IWO50_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0357997.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(31847..32998)
                     /locus_tag="IWO50_000859"
     CDS             complement(31847..32998)
                     /locus_tag="IWO50_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0357998.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(32958..34319)
                     /locus_tag="IWO50_000860"
     CDS             complement(32958..34319)
                     /locus_tag="IWO50_000860"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0357999.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(34316..35317)
                     /locus_tag="IWO50_000861"
     CDS             complement(34316..35317)
                     /locus_tag="IWO50_000861"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0358000.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(35333..36028)
                     /gene="dapD"
                     /locus_tag="IWO50_000862"
     CDS             complement(35333..36028)
                     /gene="dapD"
                     /locus_tag="IWO50_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0358001.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(36034..36750)
                     /locus_tag="IWO50_000863"
     CDS             complement(36034..36750)
                     /locus_tag="IWO50_000863"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0358002.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(36747..37628)
                     /locus_tag="IWO50_000864"
     CDS             complement(36747..37628)
                     /locus_tag="IWO50_000864"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0358003.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(37631..38431)
                     /gene="dapF"
                     /locus_tag="IWO50_000865"
     CDS             complement(37631..38431)
                     /gene="dapF"
                     /locus_tag="IWO50_000865"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0358004.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(38418..39677)
                     /gene="lysA"
                     /locus_tag="IWO50_000866"
     CDS             complement(38418..39677)
                     /gene="lysA"
                     /locus_tag="IWO50_000866"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0358005.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
BASE COUNT        11100 a         8391 c         7240 g        13390 t
ORIGIN      
        1 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
       61 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa
      121 ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata
      181 gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg
      241 cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt
      301 tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca
      361 ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct
      421 catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag
      481 catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg
      541 ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag
      601 aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt
      661 tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat
      721 agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt
      781 gccaaattat ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt
      841 gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc
      901 ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc
      961 ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctat
     1021 caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt
     1081 gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt
     1141 atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc
     1201 ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc
     1261 aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata
     1321 atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat
     1381 attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc
     1441 tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa
     1501 catggtggat tctccattcg ttatttattt taatacatca tttagagctt cactcattgt
     1561 cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag
     1621 cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa
     1681 gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc
     1741 tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc
     1801 ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac
     1861 agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg
     1921 ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca
     1981 aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag
     2041 tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc
     2101 caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc
     2161 tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc
     2221 atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc
     2281 agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc
     2341 caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat
     2401 cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat
     2461 tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt
     2521 attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc
     2581 agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc
     2641 tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt
     2701 agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac
     2761 gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc
     2821 aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct
     2881 accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta
     2941 tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc
     3001 ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt
     3061 aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat
     3121 cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag
     3181 agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta
     3241 ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt
     3301 ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt
     3361 ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc
     3421 tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct
     3481 ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa
     3541 aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc
     3601 ttttctacat aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc
     3661 ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc
     3721 tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag
     3781 cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa
     3841 taattttgct aattttacta acgccaactt gttctttccg aattcatcat tttctatctg
     3901 taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg
     3961 ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc
     4021 ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc
     4081 gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg
     4141 ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat
     4201 gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc
     4261 gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt
     4321 tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag
     4381 atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat
     4441 tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag cactgacaac
     4501 tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac
     4561 agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa
     4621 tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac
     4681 tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt
     4741 tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta aatcttgcgc
     4801 ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg
     4861 ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa
     4921 ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca
     4981 cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc
     5041 gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc
     5101 gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt
     5161 cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt
     5221 caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg
     5281 cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc
     5341 ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca
     5401 ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca
     5461 tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa
     5521 acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta
     5581 aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga
     5641 atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc
     5701 agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc
     5761 tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt
     5821 tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt
     5881 ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct
     5941 gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc
     6001 ttctaatttc tcaaacgttg caggttcttc ggcatagggc ccgacgtgta agatttgggc
     6061 acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt ccggaagttt
     6121 gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat
     6181 catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg
     6241 cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca
     6301 atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg
     6361 tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg gttttttagg
     6421 aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat
     6481 tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt
     6541 tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt
     6601 aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta
     6661 taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca
     6721 aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt
     6781 attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac
     6841 gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc
     6901 gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct
     6961 gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt
     7021 tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat
     7081 cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac
     7141 ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg
     7201 gaatcatatc ttcctgtttc ttgtgcccgt tttgattctt tctttgatta tcggctggtt
     7261 tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg
     7321 aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag
     7381 ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa
     7441 agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt
     7501 gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt
     7561 ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc
     7621 agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa
     7681 gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact
     7741 cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat
     7801 cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca
     7861 aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc
     7921 attagtagcg gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc
     7981 tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt
     8041 tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg
     8101 tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg
     8161 gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat
     8221 acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc
     8281 gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct
     8341 aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt
     8401 tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt
     8461 cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct
     8521 gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt
     8581 ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa
     8641 caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc
     8701 aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt
     8761 aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc
     8821 ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga
     8881 gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac
     8941 gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac taagagtaca
     9001 gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt
     9061 acccgtgctt atacgtcaga tttttcccca ttttttggcg tactgccaga tgatccaagc
     9121 ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat
     9181 ctattagcca acttcttaaa tactggtcat tggtctacgg aagattatca aaaggacatc
     9241 tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga
     9301 gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc
     9361 aaattctcca aactttcttt tgcttcatct agcaagagat cttttcctat ctgagcttca
     9421 atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca
     9481 aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac
     9541 ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg
     9601 atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa
     9661 aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca
     9721 aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat
     9781 cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata
     9841 attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga
     9901 tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt
     9961 atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga
    10021 caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag
    10081 taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca
    10141 tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca
    10201 gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc atattgtttc
    10261 atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa
    10321 gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga
    10381 gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag
    10441 atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa
    10501 ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc
    10561 agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa
    10621 aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt
    10681 tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca
    10741 tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt
    10801 gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttcctg
    10861 tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg
    10921 gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg
    10981 gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt
    11041 atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa
    11101 cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt
    11161 ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt
    11221 ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt
    11281 gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg
    11341 cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag
    11401 ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg
    11461 tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg
    11521 ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag
    11581 caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat
    11641 cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg
    11701 cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa
    11761 actccttttt ttctccgttt tttattcttt cgacgctatt aactttatca tttagaaata
    11821 tttcataggt aaaagaaaca tgacatttaa tgacaaaata acgaattatt taatatttgt
    11881 aacatttaaa caaatatttt tttgatacaa aaccataaaa gccttgttgt ataagcaaag
    11941 taatcgtttt tctatgtttt tttatttttg aatgaaaaac gcagattctg taacattcac
    12001 tgcaataatc cataaaaaaa agcaaaaaaa tttttgattt ttatcaaatt ctcttaaaaa
    12061 aatgtaaaaa atacaaaaaa accagtaatt gttacaacaa ttactggaca aaaactttga
    12121 ataatgttaa ctattttttt attttaactt tctccgcaat aatttctggg catgttccaa
    12181 ttcgtttata acgacttctt ctgtttcttt agctaatatt tcatctaata catcaagcca
    12241 tttttccggt tcttttgttc ctagacgtcc aatagcccaa gctgctgttc ctctgatgac
    12301 agggcggaca tctgttaaac attctgaaat ctcaggcaat gccgttcgat cacctagatt
    12361 agctaaagca atcaatgcgt ttctctgcaa aggtttcttc ccacgccatg aaccagctaa
    12421 atgcccaaat tgctgtttga agtctttatt agacatcgaa agcattggct ttaattttgg
    12481 atatacttcg tctacttttg gttccatctc ttcatgaaaa tgaaaatctt ttccttgatt
    12541 gtaagggcag actaactggc agatatcaca tccataaatg acatttcgca tttttttacg
    12601 atattcttcc ggcatcatcc cttttgtctg agtttgataa gacaaacatc gctgggcatt
    12661 cattcttccg tcaccaagca atgcttttgt cggacaagca gtcacgcagc gcatacagtc
    12721 accgcaacca aattctcctg gtttatcagg ctcaaataca atattagtaa cgatctcacc
    12781 aagatagaca aatgaaccga actcttcagt gatcaacaat ccgtttcgtc caataaatcc
    12841 taaaccagct cgctgtgcta cagcgacatc gatcagttct ccagtatcta cttgcggagc
    12901 tagacgccat tcctcttctt tttgccattt ttctgctctt gtttggatga attcgatcaa
    12961 tcggttcaaa cggtctctga gtatatcatg gtaatcgatc ccccaagaag cacgagcaaa
    13021 ctgcccgcgt ttttcatccc taggcacttc ttcatggatc ctagtaggat acgcaagtgc
    13081 aatggaaatg atcgatttcg gctgatcaaa tgtcatttct ggataaagac gctcatcgat
    13141 atttggatgt tcaaagccag aagtatggcc agctgctttt tgttcttcca acgattcttt
    13201 taatgaatca aaaggttccg ccgtggtgaa cccgattttg tcaattccta atcgtttgct
    13261 ttcttgtatg atttcttctt ttaatgtcat ggtcttcctc gcttgctgtt aacggctgta
    13321 taacccataa tatagttttt cttttttcag ctggacaaac tggatctgct gatcggcaat
    13381 cttcttttct tccatccatt tttttcttgc agcaaatact tcccaataat ttgttgaaat
    13441 taattttctt ttgttatcta atgcattaaa aaaattcaat gaaactggtt ttgtacttaa
    13501 cagttcatgg agccttacct cttcccatgg cgaaaactca gacagatcgc gggaatacaa
    13561 tacttcttca tagagatttt tttgctgtgt gtacgtgaat gtttgcggga ttttcccagg
    13621 cctagcaatt tctactagac gattataaaa catattcatt aatttgctgt tggcaataaa
    13681 ttgactttta taataggccg actggatctt tttgattact ccttctgctt tgatgactga
    13741 aaataatgca ggccatgctt tgacggttat ttcgtcgttt tgtttaacta atacgccagg
    13801 tgtgaagccg actggagaaa ggaacacaaa ataaccgata ccggtatttt catctaaagg
    13861 ctgaaattca tcaaacaacc atttgcctat gtacagctct tcatctggtt ctgttgtctc
    13921 attttgattt gctatgaata atttagctaa ttctgcttca agaaattgaa ggtcttgatc
    13981 cttgtcggta ttttcctgct ggctgatcaa tcgaaagact tcttctttaa tcgtatattt
    14041 atctttacgt gtaaaattca tagccacacc ttccttcctt actctaacta taacggaatt
    14101 tcttcaaaaa aggtatctgt acgacgaaaa acagcataat tttttttcat atctcttata
    14161 aaaaatgcaa ggtctgctcc tcaagctaga ccttgcactt ttcagctttc ttttgtttgt
    14221 gacaaacctt catggatcgt ttcaatattc catttcatca tctgatagta agaatctcct
    14281 tgttctcctt cttttgctat ggaatcagtg aacagtttat catagatttt caaacccgtt
    14341 tcttttgcta cccgttccat gcttcttgag tctacactgg tttccacgaa taaaaccggt
    14401 acttcttttg ctcttatctg atcaatgatc gctttcattt gatcaggcgt accttgactt
    14461 tctgtattga tctcccatat ataagctgct ggcaaatcat atgctcgtga aaaatacttg
    14521 aatgcccctt cacttgttac taataatttt ttgttctctt cgatagaagc aaaactttcc
    14581 ttcgcttgtg tatctaattc ttttagtttt tccacatatg cttctgcatt ttttttatag
    14641 agtgtcgcat tttctgcatc tttttcagag aatatacgtg cgatttcctc cacatatttt
    14701 attccgttag ataggtctag ccaagcatgg ggatctgctt ttgtatgttc ttcaccgcta
    14761 gttaaataaa gaggttctac atttttgcta actgcaaaat aatcttttcc ttcttctttt
    14821 ttagccgttt ccatcaagtt atcgaaccag ctgttacctg tttcaagatt caaaccgttg
    14881 tataaaataa catctgcatc acttgccttt ttgatgtctt ctggtagtac ttcatattca
    14941 tgcggatctg ttccgactgg tacgatactg tggatatcta tttgatctgt tccgacctct
    15001 tttgccatgt ccgccaagat cgaattggta gccacgactg acaacttttc tttacttcct
    15061 tcatccgctt ggtcagaagc ctgctttccg caaccggcta ataccaagaa actaactgcc
    15121 aagattaaaa agagtgattt ttttgttttc atctatctgc ttcctttctg aaataaaatt
    15181 ccttttgttg gagaaaataa aaaagcaagg ataaaaaatg cggtacatgc taacacgatc
    15241 gtagctcctg atggccaatt gtaactataa ctgaaaaaca caccgacaac tgcacttagc
    15301 ataccgattc ctgcagctgt aatgatcatt ttcagtaaat ggttcgttag caagtaagcc
    15361 gtggcggctg gtgtgatcaa catggcaatc accaagattg ttcctactgt ctgtaaactg
    15421 actacagcca ctaatgtcaa aaagaacatc aaaagataat ggatcagcga tgtgttcagc
    15481 ccataagctt ttgccattgt cggatcgaat gatgtgatct tcaattcttt ataaaagaaa
    15541 aagacaaata tcaagacgat tccactaacg agtgctgtca gcaacaaatc gctttcccta
    15601 acagctaaga cattcccaaa taaaatatga tacaaatctg tcgcactctg ggcatatgaa
    15661 atcaagataa tccctaatgc aaaaaaggag ctaaagacaa ttccaatcgc tgtatcattt
    15721 ttcaatcggc tccgctgagt cacgaaacca atcactgctg ctgttagcat cccaaaagca
    15781 gtggctccaa agatgtaact aaaaccaaac atataagaaa tggctacacc tggaagaact
    15841 gcgtgagaga tagcgtctcc catcaaagac atccctcgta aaataataaa agaaccgatg
    15901 actccagaag caagtcctac aagcatggaa gtgaccaaag cgtattgcag gaattgataa
    15961 ttcattaatc catcaataaa ggattggatc attttttctc tcctcctttt actaaatgtc
    16021 tgatgatctc accataggcc aattgaaggt tcttcgatgt aaagacttct tgtactggac
    16081 cggaagcgat cagctgttta tttaaaagaa taacctcatc gaaatattct tctactttat
    16141 gaagatcatg atggacgatc aaaatcgttt ttcctgaatt cttcaattcc tgcaagatgt
    16201 caaagatctt tcgttcactt aacgcatcaa tccctacgaa tggttcatct aaaaagatcc
    16261 attctgctcc ttgggctaga gctcttgcaa taaaaactct ctgaagctgt ccacccgata
    16321 gttcgctgat ctgtcttttg gcatattctt ccatccctac ttttttcaaa gcttgtcttg
    16381 cacgttcttt ttcttttttc ccaggtcttt gtccaattcg taaagatgga tatgttccta
    16441 aaagtacaac gcctaataca tcaattggaa aggaaagatc caattcactt cgctgttcca
    16501 cgtaggctat ttttttacgg actgagttta ccggctgtcc agaaaaggtg actttaccag
    16561 tagtagctgg tatcaactct agcaaagatt tcataaatgt tgattttcct gcaccgtttg
    16621 gtccgataat accggtaatt ttttgtggat ttatcgtcaa gttgatagtt ttcaaaacag
    16681 ttttcccttg ataagcggct gaaaggtctt gaattacaat ggccgtacgt tccaaatctt
    16741 acactccttt ttaggtttac ctaacttttt atttaaatat agtttaacta aaatggaaag
    16801 cattttcaag tagaatgatg aaaaagtttt tttcgaattt tcctcgcatt ttatgaaaat
    16861 tttctgtaga atcacatcaa gagttcaata aggctaacct aataatgaaa aacatgtgtt
    16921 gaaacgtttg attatttttt cttgttgact caacaacgaa aggagttttt gtatggaaga
    16981 aaaaaagtta actcgaagtc ttagtgcccg tcatatccaa atgatcgcac taggtggaac
    17041 gatcggtgtc ggtctgttta tgggtgcctc ctcaaccata cgttggacag ggccctcagt
    17101 gatgggagcc tatgcgattg caggattctt tttgtatttg attatgcgtg ctttaggcga
    17161 aatgctttac gttgatcctt cgactggttc ttttgcaaat tacgcgagcg agtacatcca
    17221 tcctgtggct ggctacttga ccgcttggag taatattttt caatatattg tcgtaggaat
    17281 aagtgaagtg attgctgtag gatcttatat gaattattgg tggcctgatt tgccggcaat
    17341 tattcccgga atcattgtcg tcttgtttct tatgctggca aatcttattt ctgtcaaggc
    17401 gtttggcgaa ttagaatttt ggttttcgat catcaaagtc ataacgatca ttttgatgat
    17461 cattgcaggt cttggggtaa tcttatttgg atttggtaat catggtcagg cagtagggat
    17521 ttcaaatctt tggaaaaatg gtggattctt cactggaggc gtcaaaggat ttttctttgc
    17581 attatcgatc gttgttgctt cctatcaagg aatcgaactg atcggaatga ctgctggtga
    17641 agcagagaat cctaaaaaga cgatcattga agctgtccag tccactatcg gtcgaatctt
    17701 gattttttat attggtgcta tttttatcat tgtgagtatc tatccgtgga atcaattgag
    17761 tgagattggt tcaccttttg tacaaacttt ttctaaagtc ggcattacat ttgctgcagg
    17821 actgattaat tttgtcgtga ttacagcggc attatcaggt tgcaattcag ggattttcag
    17881 cgcaagtcgt atggtctata cacttgccat aaatggaaag atgtctaaaa aatttctgaa
    17941 actatctcgt cacggtgcgc ctttttatcc tgtcgtagca atctcaggtg gaatcttaat
    18001 tggtctgatc ttaaattatc ttctaccaat gttcatcaac cagtcagaaa atattttcgt
    18061 tttcgtatac agctcaagta ttttgcctgg tatggttcct tgggtagtga ttttgatcag
    18121 cgagattcgc ttcaaaaaga agcacgcaga taaaatgcat gatcatcctt ttaaaatgcc
    18181 actatctcct tatacgaatt atgtgacact tgctttttta gctatcgtat tgattttcat
    18241 gtttatcaat cctgaaacga gaatctcatt gattattgga ctcgttttct tgctttacat
    18301 gacgatccat tactttgtac gcgaaaaaag aaccggaaaa taaaaaaaga acgaagcttc
    18361 accttagtgg aagcttcgtt cttttttatt gcttttatca attcaatttt agcgtctcac
    18421 cgggagtcag gaaataattc tccatgctca tcccattcag cgacaagatc gtttctactg
    18481 gtacgccagt tcgttcggct atttgatttg gaccttctcc ttccaaaact acggttgtcg
    18541 cagcctgtga agtcgcgctg ttggtattag tttcagagga atctgttgtg tcttgtgttg
    18601 ttcgagcttg catttcagaa gcttctaccg actctgctgt ctcagaatca gaagatgttg
    18661 tgtcttgtgt gcttgttgac tctgcttgaa gagaggcgct agtctgcaaa taaatatcgg
    18721 aaatcttagc gacatgattc gtataattag ccgataattg attgatcaaa gtatctaagt
    18781 ctacaccgtc tttattttct gctgcctgat acgctgcacg taattttctg atcccttcgt
    18841 cggagattgc ttgaagctca cgtgcttttt ggttagcaac tgctgataaa ccagctattc
    18901 cctcttgatt tccctgaatt tctgtctggt attcttcaat caatgcaggc gtcttttctt
    18961 cgatcttttt cgaataatca tttacgattg cttgaagttg ctctggcaaa ccatttgctg
    19021 ttttttcttc agaagtttgt gtctcttttg ttttagttga tgactcactt gtttgtttcg
    19081 tctctgtttt ctttgtcgtt gtttccgtta tggtcgtact tttcgcttct gacgaatttt
    19141 ttccatgaaa atatagaccc gcaactcctc ctgctatgat caaaaacacg gcaactgctg
    19201 ccccaatcat gatcatccgc ttacgagctg cctgtttttg ctgtcttctt cgggtcatcc
    19261 gaacattttc tccccactca ttttcatcat acattttttt caccaccaat ttttatgtat
    19321 aatagcgtta tcaaacgctt catattctta ttgtagcaaa taattgtcgc tgtgcacatt
    19381 tttatctttg ttatttataa gaaaatttca ccagttgaga tgattccaag attgtccgtc
    19441 gatttctcct tgcagctgca ttgttcgttc gtctatctca gccatcagtt tctttatttc
    19501 atataagagc gcacggtctt gatattctga tttttcgctt aattcatcta attgttggat
    19561 cagccaatca tttgtcataa tgaagttcct ttcatttctt gcagtgtaaa ggtcaagtct
    19621 tcgatctgct cttttgcctc agtcagtgct tctacgatat atgcttgttt ttcagattct
    19681 tcttcctgca ttttctgatt ttcctgtact ctttgaataa accagtcaat ccgctgagtc
    19741 agcgatggtt tgccttccaa tgagagatac tgctgataag caattagctt ctcccaacgt
    19801 ttttcctggg ataaatacat aaattgtgct aaaacgaatg gcggaagata ggtcatatgg
    19861 aatttatttg cgattcgctg atacggcctc aaaaattcgg aaatcgtata ttcttctttt
    19921 cctccagctt ggtattcttt ttccgccaca cctgttgtca atacgattcc tagttctttc
    19981 ccttttaata catgtgctgc tcgttccaag acattatctt gccaaatttt taaatgagca
    20041 ggagaactat accagtaaaa cggaaactgg aaaatgatcc gatcatgctg ggccaagagc
    20101 tgtagttcag tctctatatc tatttttccg tctggataac ttgattccaa atgatgccaa
    20161 gtagctggta aaccagcaga agcttctttg aaaaattgct gatttaaaga ttcttcgatc
    20221 gttggatggc taatgatgat aagtgtacgc atcccttttc ctccttttta gtcttcgctt
    20281 tacagtgtag cataaccgcc acaagtttct tttgccgatg cttgtcattt taaccagact
    20341 aatcaggttg tccaagaaac tgcggtttgt ttttccaact gttttttcaa ggtatccaaa
    20401 gcaatattct taaaattttc gtttttcata cgtttacgat agttcgtagc cgtttctccg
    20461 gtactctttt tgaacattcg gctaaatacg actagactat tgaaccctac ttgataggca
    20521 atactttcca cggattcatc cgtcagagaa agaagaaacg cccctcgatt gatccgtacc
    20581 ccgatcaaat attccttgat actggtaccc atatgatttt tgaatactcg cgacaaatgg
    20641 ctccgattga ccgctaaatg ctgggcgatt tcctcaacag acaatttttc agcataatgt
    20701 gcctcgatgt atttgactgt ttcaacagct aaagaggaat gaaccttttg tacaccaccg
    20761 ctgacctttc cgccatcttt taaaaggact gataaaaaac ggtaggttaa tgctgttaac
    20821 tgtaattcat tttccaaggt ttcttccgta tctttcatgc attctaaaat aatgtcgata
    20881 tatttttgta tgtcagaaga gaccatcgtg tattggtctt ctctgaaaag cgactgccga
    20941 atgatttctt ctgcagcact cccaccaaat gaaagccagc aatacatcca cggatcttct
    21001 ccatctgata agtaaaacgt cgagtctcct ggacgaatca agaacagatc accttttttc
    21061 aattggaatt gttgattttt cacatgataa gatcctttgc cttccatcac gacatgcagg
    21121 atgtaatcat tgcgaatagc aggaccaaaa gaatgaaacg gcatcgtttt agagaaacca
    21181 caaaaatcaa aatacaattc tttttctact tgttgatgtt ccttttttaa aaaaatcgat
    21241 tgctccacgt ctatccctcc ttactccttt agtataatgc aacaaaaagt taaataaaag
    21301 tcacagaaat atattaaaaa ggaagcgatt tcttgttaag tttaagtcat taaaagatct
    21361 ttgttattcg ttgtaaccgg tttacttagt attatatatt gaacaggaga ggaatacaga
    21421 ttatgaaaag caaaatgatc caacgtgtgt cctacgcttt aggcgctttt ggacacgaca
    21481 cattctatgt tacattaagc acttatttta tggtattcgt gacaagccag atgttcgcag
    21541 gaacagataa ggcaacgaat gcaaaaatga ttgctacagt tacaagctta gttgtaggta
    21601 tccgcttagt tgaaattatt tttgatcctt tgattggtgg catcatcgat aatactcgta
    21661 caaaatacgg taaattcaaa ccatggttag ttatcggcgg cgttgttagt tccgtactct
    21721 tggtagcaat ctttaccaat ttctttggct tagctactca taatacgact ttatttatca
    21781 tattatttgc aatcgttttc atcttattgg atagttttta ttcatttaag gatatcgctt
    21841 tttggtcaat gattccagct ttgagtacgg aaacaaagga acgtgaaaaa tttgctacgt
    21901 ttgctcgttt cggctcttct ctaggtgcaa atggtactac tctagtcgtt gtccctatcg
    21961 ttactttctt tacctatctt gttactcata aacatattga aggcgcttca ggctggttct
    22021 ggttcggtgt gattgctgct gttatttcag gcggtactgc gattatcact gccttgggca
    22081 caaaagaagt cgatactgcg atccgtcagc aaaatgaaaa agctgggatc aaagatgtgt
    22141 tcaaagcagt tgctcaaaat gatcagttga tgtggcttgc ccttgcttat atcgcttatg
    22201 cgattgccaa tgtagcaaca actgccgtat tattctactt cttcaaatat gtacttggcc
    22261 aaccaactga attttggatc gttggtctag ttgctgcctt acttggttta gtagctgttc
    22321 cactattccc aatgcttaca cgcgtcatct ctcgtcgcta cgtttacctt ggagggatcg
    22381 caatgatgat ttcttcttac tttttcttta cgatcgcagg ttcaagtttg ccaatcgtta
    22441 tcatcgggct agtattgttc tacttccctc aacaattgat tttcttatcc gctttgatga
    22501 cgatcacaga ctctgttgaa tatggtcaat ggaaaaatgg tatccgtaac gaagctgtta
    22561 ctctatcaat tcgcccttta ctagataaaa tcgctggtgc tttgtctaac gggatcgtag
    22621 ggtttgtcgc tatcgcttct gggatgactg gtaatgctac tgctgctgat atcactgctc
    22681 atggcgtaac gactttcaaa gcttatgcat tctatgctcc tgctgctttg atgattttct
    22741 ccgctattat ttttgcttgg aagattacat tgacagagaa aaaacatgca caaatcgttg
    22801 aggaattgga aaacaaattg gctccagctg atacaatgga agatgaactt cacgatgcag
    22861 tagttccttc ccattaagaa aggatcttga aaatgaagat tacagaaaaa gattttggcc
    22921 aaggttatca cttgataacc ttggagaaca aaaacaagct agcactttct atctctgatt
    22981 taggtgctag aatcgtcagt ttaaaatcaa atgatcgaga attagttttg ggatttgaca
    23041 ccgctgaaga atatatcgaa aaagatccgt atatcggtgc ttctatcggt cgcaccgcag
    23101 ggagaatcga aaatggccgc ttttctctca atggaaaaac ttaccaatta gccacagatc
    23161 caaaaactgg acataatctg catggtggtg ctcctggatt tgaattaaaa aaatggtctt
    23221 atgtcatttt gaatggcgaa aatgaagctt ctgtcatttt tactactaca agtcccgacg
    23281 gagaacatgg ttttccaggt acaatggatg tggaaatccg ctatactttg acaaaagata
    23341 atatctggcg agtgacctct cgcggcacca gtgaccaaga tactttattc aatccgacaa
    23401 atcatgttta cttcaacttg acgggagatg cttctcagtc aatcgatcaa catgagcttt
    23461 ggctcaatag cgaagcttat gctcctttgc gaacagatag tattcctata ggggtaaaag
    23521 aaaatgctgc aggaagtgct ttcgattttc aaatacctaa aaaattagcg tccgtttttg
    23581 ctagtgattt ggatcaaaaa aatcttgttg acggaatcga tcatccattt ttcttaaaag
    23641 aaaccggctt gggtaaagaa gccgctcgct tgactagccc cgataaaaga atccaagtag
    23701 gtattgctac agatgcttct agtgtggtta ttttcactgc aaatttcggc acagaaacac
    23761 cagaaatgcg caaccgaaaa ctggcacatc acggtggcat cacatttgag actcaaacag
    23821 ctccaggtgc agagcgcttc tcagcatttg gttcgattca tttaaaagcc ggatcagtat
    23881 ttgaaacagt gacagaattc aaaatcaaaa caagaaagga ataactcatc atggaaatca
    23941 aaagaagctt aaacacaaaa tttgccgaaa cattcggacc taaaaaaact acacaatact
    24001 tctcaccagg acgcatcaat ttgattggtg aacacacaga ttacaatgga ggccatgtct
    24061 tccccgcttc catcacttat ggaacgcaag gtgtagcagc acctcgagac gataataaaa
    24121 tattagttta ttccacaaac tttgacgata tcggtgtcat tagttttact ttagatgagc
    24181 tagaatatga taaaaaagca aactgggcaa attacgtaaa aggaatggtc cttaaattaa
    24241 aagaagctgg acactcaatc gatcatgggt ttgagttgtt agtggaagga acaatcccta
    24301 atggtgcagg actttccagt agcgcttcat tagaattatt agtaggtgtt gtattagaag
    24361 atctgtttga tttaaaaaca gatcgcttgg aactagtcca aacaggtaaa aaagtagaaa
    24421 atgaattcat cggtgtcaat tcaggtatca tggatcagtt tgccatcggc ttcggtgagc
    24481 tagatcatgc aatcttatta gatacaaata cgctgaaata cgaaatggtt ccagtgaaac
    24541 tagatggtta tgcaatcgtc atcatgaata cgaataagcg gcgggaattg gcagattcaa
    24601 aatacaatga acgccgtagc gaatgcgaag aagccttggc acgtttgcaa aaaactttgc
    24661 ctatctcttc tttaggagac ttagatgaag aagaatttga atcaactatt gaccaaatcg
    24721 gcgatgatac attgatacgc agagctcgcc atgccgtttc tgaaaatcag cggactttga
    24781 aggcaaaagc tgaattagaa gctgggaact tagaagcatt tggtcaactg ttaaatgatt
    24841 ctcaccactc tcttcgctat gattatgaag taactgggat cgaattagat acactagtag
    24901 atgctgctca aaaacaagaa ggtgtcttag gtgcacgaat gacaggagct ggatttggtg
    24961 gctgtgcgat tgctcttgtc aaagaagaaa atatacctgc cttcaaaaac aatgtctatg
    25021 atgaatacat gaaagttgtc ggctatgctc cggaatttta tgttgcacac attggaaatg
    25081 ggacaacaaa gatcgatcca gaagctgttc atgtttgata aaaaaatcaa cgatctcagc
    25141 taaaaacaaa tagataaaaa taacctctca gcaatttcca aatgtgagcc ttttgctcgt
    25201 atttgaaaat tgctgttttt tattgacacg gaaagagaac gtgtgttcta attgtgttga
    25261 agaggtgatt gttatgtggg cgaatcaaaa tccggtattt gtttatgaaa aagaaccttc
    25321 aagagatatt ttatgtattg actgcaaatc tttttacgcc tccgtagagt gcgttgaaaa
    25381 ggatcttgat ccccttacaa ctaaattagt agtcatgtcc tatccgtccg attcgacaga
    25441 aacacggggt agcggattaa tcttggcttc cagtcctgca gcaaaaaaag cttacggcat
    25501 caccaatatc agccgtgcca gagatcttcc cttcccctat ccaagtgatt tatatattgt
    25561 tgccccaagg atggcctatt atatggaaaa gaacagagaa atcaattcta tctataaaaa
    25621 atatgcagac gaagcgaacc atcatgtcta ttccgtcgat gaaagcttcc ttgatgtaac
    25681 aaatagcatg gcactctttg gtaaaacaaa tgcgtacgac atggctaaac tgatccataa
    25741 agatgtgttt gagcagaccg gaatctacac gactatagga attggcgata acccgctact
    25801 tgctaaacta gccttagaca atggctcaaa agacggaaag caaatgtttg ctgaatggcg
    25861 ttatgaagat gtaccagaaa agttatggaa aatctcagat atcactgact tttggggaat
    25921 cggcaaacga acagcaaaac gtctgaaaca aatgggtata cattctgttt atgatttagc
    25981 tcacgcggat tattatcact tgaaaagcaa gctaggggtc atcggtactc agctttatgc
    26041 ccatgcttgg ggcatcgatc gaagtttttt gggacaagaa tatacaccaa aatcaaaaag
    26101 tatcggaaat agtcaaatcc tagatagaga ttatacaaga cgagaagaaa tcgagatcgt
    26161 tatcaaagaa atggccgacc aagtcggcac tcgcttgaga cgatctgcag caaaaacaca
    26221 agtagtcagc ttatgggtag gatactcttt aggctatctt gatccaaatg gcaagaccgg
    26281 gtttcaccag caaatgaaaa tcgaagcgac aaatgctagc agagatctag cagatgcttt
    26341 attgaccatt ttccaccagc attaccacta ccaggatatt cggcgtgtag gcgtgaactg
    26401 ttccgacctc gtatacgcga caggtttaca gcttaacctt tttgaagatc ctgaaaagca
    26461 gataaatgaa gcgaaagttg attttgttgt cgatacgatt agaaaaaaat acggctttca
    26521 atcaatcatc catgcgcata gtctcttgca aggaggacgc gccatcgcac gtagttctct
    26581 cgttggcggg catgcaggtg gtatgagtgg attagaaggt ggaaaagatg agacggacag
    26641 caaaaaaatt cattgactat aacgagtatc gagatcggcc atttggatta aaatggggga
    26701 ctgcttttgc gatggaagaa ctagtcaacg gtatcaaaaa aaatgaagaa gaagcacgca
    26761 aacagcctct tcctaaacaa caaatgacat tgcaagaaat cgatagtata ctgacagaag
    26821 cctttttatc tcgtaaagca gtaatcattc agctcaattt aaaagatgaa tttggaagac
    26881 tcttggatga ggtcgaaggg cacttcattg gtgaagccta tgaagattac tttatgattg
    26941 accatcagcc gatattgtgg gaagacgtac ggtatattga gttaaaggaa acaaaaaaat
    27001 ggtttgtgac atgagaggtt gtgacagaag tgatcaactt caaaaaataa gaaaggattt
    27061 ttcgaaaatt gcttcttaaa tttttgtgga attttggctt atttttcgaa gaagcaactt
    27121 ctggaacaac gtatattaga tatcagaggt tgtgaaaaag tcgcttagtt ttgagtaaca
    27181 agccaaggcg ctgatttcac aacctctgtc tatctatcca tctattcggt aagattcatg
    27241 catgacgtct tctttattta gtatgtagag ccctttttct tctgaccatt caacataaat
    27301 cagctgtttt acatctgcat agccttcttt cttcaagttc tccataagcc atgcttcagt
    27361 caattgattg gactgaagca ctttatgctg aatttgccct tcatctacaa gcaggatcga
    27421 taaagctttg tcttcatctg attttttcaa tacactcatt tgaccggcat tctctaggat
    27481 aacatgtttg gcattttcaa gagaaaagca ttcttgctgt cttaagattg ctcgcaactg
    27541 ctccatctcg atatggttat ttgtcaactc tgtcatgttg atgacgccgt ctttgattag
    27601 aacagatggc tcgcctttga cccaacgatt gaccttttca tttttttgga cgatacgttc
    27661 gataccataa atcattactg cccaaagcgc aatggcaaat aggacatgcc ctacatggac
    27721 attgtcatca taggttgatt cttctaagat cccacctaaa acaagcgtat agactaaatc
    27781 aaacggtgtg atttctgaca tggatttctt cccaagcaaa cgtaccacta ataataaacc
    27841 gatcaacccg aacaccagtt tcattgtaat tgaaaaaatc attttttctc cccctaacta
    27901 tctgttaatt aaattatata tgctttctca cgaacacgca tggaatatac aaggaagaaa
    27961 tgtatccttt tactaaaaaa aggactgtga cactgtagtg acccccaaaa gttagacttt
    28021 tttggagtgg agaaatccac tccttttttt tatgctctaa aatgatattt ttacacaacc
    28081 gttacggttg ttttacgaaa ttggacgaga ctttgccagt ttaatttctg ttttatccgt
    28141 tcattattgt agtaatagat atagctatcg atggctgatt ttaattcctc gaaacagtta
    28201 taaactttcc cgtgaaaaat ctcttgtttt aataagccaa aaaaattctc cataagtgaa
    28261 ttatctaagc agttcccttt ccgagacatg ctttgaaaga ttttgtactc cttcaatttg
    28321 tttctataag ctttcatttg atacgcccaa ccttgatctg agtgaaacgt acgacgatag
    28381 ggacaatcgt tagtggtctg gatagcttcc tccaaacctt ccattaccgc taatgcattg
    28441 ggcttttcag agatacgata ggataaaatc tcactattga acatatccat aaaaggatct
    28501 agatacaatt tcttttgacg gataatgcct ttggcatcca cttcatggta tttaaattca
    28561 gttgtatccg tcgtgatttt ctgatggcag atactggtgt agaaacgacg atggatccga
    28621 tttttggcta ctgctccaac ttttcctcga taagaattgt agcgacgtga tttacgcgtg
    28681 tatgccgtta cttttattcc tagcttcttg atcaagcgct gaactttctt tttattgata
    28741 gagaaaccgc ggtttcttag ttcattggtc atccgtaaac agccataatc cttgtgcttc
    28801 tcccgaattt taagcatttc cgcttcaatc tcttgatctg gatttttacg gtccaatcgc
    28861 ctttgccatc caaataatca tgaacgatct ttaatttaag ttcaaaagta tatttaacca
    28921 taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg gtcggtacac
    28981 aaatcgtgtc acagtccctt ttttgaaaat aaacggtgtt caaaagcttg catctgcggt
    29041 aataagtcca gccaagttaa aaacataaga agctgttttt acttggctgt tcttattact
    29101 tgatgcaagg cagctttttc acaacctctt ttcaaatatt tcgttttctt attctacaaa
    29161 tggttcttct cctaacgctt gttgagcggc tagattcaac atgctccatt gtcggtcaaa
    29221 gcctggctgg aagaagaagt cagcttctgc tagttcttct aaagtcagtt tgttttggat
    29281 cgctagtgcc aatacatttc cttgacctgt aacatcatag gtagataata ctgctcctcc
    29341 tagcaattga tgggtcattg gatccgagaa taattggatg gacacttttg gatttccttg
    29401 ttcttctggt acatattttg gtcgtaacgt atcttcataa taagacactc gtacatctac
    29461 tcctgctttt gctgctgtaa agctattcaa tccgcttgct gcaaaatgat agtcaaagac
    29521 acttaatgca gaggaaccta cgactccgcc gaacggtttg tttggtactt cttcaaaaag
    29581 atgttttact acataacgtg cttctctacg tgcaacagtc gctaaagcga tcggcatttt
    29641 tttgcgtgct gggatagaat aagcaagtgt agcatctcct actgcataca catctggaag
    29701 attcgtttgc aagtattcat ttgtttggat ccagccacgt tcatctaagt cgacgatccc
    29761 ttttaaccat tctgtatttg ccttcacacc ggcagcttga atgactagat ctgctgggat
    29821 ttctttttca cttgttttga ttgcctcaac ttttccgtta ccagtaaaag cttcgatttt
    29881 tgcatttgtc aaaacttcaa taccgttttc atttaggtgt tgttctaaaa tatctgtcat
    29941 ctcagcatct aaataagtgc ctaaaggtct atcgatcaca tcaagcaata cgacttcttt
    30001 cccagctttt cggcttgctt ctgctgcttc gatcccaatg taacccgcac ctacgacagc
    30061 gattttttta atagccggat catttaattt ttctttgatt ttggttgccc aattgtatcc
    30121 acgcatcaag tagacatttt ctagatctgc tcctggtact ggcagattgt ttggtttgac
    30181 accagaactc aaaatcaatt tgtcataagg atattctttt gtttcgttat ctgatgcgct
    30241 agtaaccgtt actgtttttt tatcggcatc gatcgttttt acttcgtgat tatttaaaat
    30301 atgaacattg gattgcggaa agttttctgg tctgaaatta cgtacatcat ttacatctgt
    30361 taccttgtct tctaaataca gctccatacc acatgacatg aaggaaatat agtctcctgc
    30421 ttcaaatagc gtgatatcta cattttcacc gtatcgtgtc aatagttcca agatcgattg
    30481 atgccctcca tgagatgcac ctacaatgac aactttgatt ttttccattg atgaaacctc
    30541 cttgaatatt cttttaaaat cattctaaac aatttaaact taacattaca ttagttatga
    30601 atcaaaagaa acgcgttcat ttgaactaaa tttaaaaaac aatcgtctcc ttgcagaata
    30661 taccgcaaaa ttaacgattg tttttttgat aaaatctatg aatcactgtt ctgttaaaaa
    30721 gtctttgatc cgtttgcagg cctcatgtaa tttttccatg ctgctagcat aagaaatccg
    30781 gatataacct tctccttctg ggccaaatgc aattcctggg atacacgcaa cttttgcctg
    30841 ttccgctaat tctacacaaa actgccaaga gtcagtttga atggcagctg ggagtttgca
    30901 aaacaagtaa aatgcaccgt ttggctgggt cacttcaaat cctaaaggcg ccaattgctg
    30961 gaccagatag tctcttcgtg tttgatattg ctctttcata ttctcgctat ctttcacccc
    31021 atttattagc gcttcagccg cagctttttg cgacatcgtt gtagcggcag tcaccagata
    31081 ttgatggact ttaattagtt cgtcaataag ggctttttga gcaaaaataa agcccactcg
    31141 ccaaccagtc atcgcatgtg acttggataa cccgcttatc acgattgttt gttctggaag
    31201 ataagtagct atggacatat gcgttccact ataagtcaat tcagcatata cttcatcact
    31261 tataacaaat accggatatt tttttaaaac ctcagcgatt gctttcaatt gttttgcaga
    31321 caatgtcgtc ccagtaggat tgttaggata gtttaaaatg actgctttga cagctgatcc
    31381 atattgttca aatgctcgtt caaattgttc cggctgacag acaaaccctg tgtcagaagt
    31441 atctatcggg atcaattcag caccagctag ttccactaat ggctgatatc ctgaataagc
    31501 aggcgctgga ataagaacct tgtctccttc ttccaaaaca gataacaacg cagaggcaat
    31561 cgcttccgtt gcaccaactg tcgtcaaaac ttccgtttgc ggatcataag ttaatccata
    31621 acgctgttgc tgaaatatac aagccgcttc ccgcagttcc tctaatcctc tcattccggt
    31681 atagtgagaa aagtcttctt caatcgctgc tattcctgct tgcttgacat gttcaggagt
    31741 tggaaaatcc ggttctccca gggtgagttt gatcacatct ggtatcgacg atatttgctg
    31801 atcgaattgt cgtatttttg atacttctat tctagaaagc ctaggactaa atcgattgga
    31861 taaattcatc attatttact cgctcctctt ttgaactgct atcgattttt acaagaagat
    31921 tttctgtaaa aagaatcgtt ttgtaccatt ctacaccatt ttctaaaaca tccatatcaa
    31981 aatcgaacgt cggatcatgt aaagcagggg tatcaccgac acctaaaaag aaaaatacac
    32041 atggatattg ttgcgtatat atgccaaaat cttcggcttg aagaactgga gcatctaatt
    32101 catgtagttt gtttgaatct aacagtgctg catataagcc tctatggttg attacagctg
    32161 gatagccgtc attatagcga aaagaaatag tagctgtaga gtgaaactgt tctgctaaat
    32221 ggtctaatcc cctcttcaaa taattttgca tatctgaact gtaagaacgg atacttccgg
    32281 atacaataac ttctccagct aaaacatttc ggatcgttcc accttggatt tggccaaatt
    32341 tcattagatg attccactct gccggtaatt gccggtcaaa atcataaaca ttcttcaaaa
    32401 atcgtacagc tgcctccaga ctatcgattc ctttttcact agaagcaata tgtgcagact
    32461 ggcctttaat gataatgtct gtttcactgc tttgtgccat caaaggtcct ggtcgagaga
    32521 aaacttctcc cttagctagt ccaggccata gatgaaagcc aaatattgct tgtacttcat
    32581 atttatccaa caatcctgac tgaataacgc tttgtgcgcc accggaaatt tcttctgatg
    32641 gctgatagat cagtactaca ttataaataa gatcctcaat gtgtttagtt ataaagtcag
    32701 tcaaccctaa aagcatcgcc atatgtccgt catgaccaca tgcatgcata taaccttcat
    32761 gcttagaagc aaaagagaga ttcgtttctt ctttgactgg cagtgcatcg atatctgttc
    32821 gaaaagcaat cgtttcttct tgctgattat caaaataaag gagtagtcct gtttcgccga
    32881 tttcatggat aacggccccg ctgtttttta cttgttcata taaatatttt tttgtcttga
    32941 attcatgaaa cccgatttca ggaatttgat ggagtgccat tcgatattct ttcagcatcc
    33001 gtattcacca tcccttcata gatacccttc actactgttt catattgatt attcggtaca
    33061 ccgatgatca gattcaactc ctgtgctcct tgtgataaga tcgatgtgcg aatttctaat
    33121 ttgttcaata tcgataacac tttcccagat aatccaatca attctttatg cggaccccca
    33181 acgacagaga tcaaagctaa gtcttcgatc acgacgattt cttccacgcc aagtgtttct
    33241 tttaattctt tcgtgatgag aaataactta tcggcaatcg cttctgctga gacgatcact
    33301 ccgatattgt cgatccctga aggaatatgt tcgattgata tctcatgttt agcaaaaatg
    33361 ctcattgctt tccagataaa cccgatttca tcggacatat gacgtttgaa taaagtgatg
    33421 gaaagaaaat ccttgcgtcc agctattcct gtcagtccat tttccttttc tatttcgtga
    33481 ttcgatattt ttgtcccttg agcgaaagga tcattggtat tttttatttg gatggggata
    33541 tcagcctctt gaactgggaa aacagctgct tcatgaagaa cgttagcccc catataagcc
    33601 atctctctca gttctctaaa agagatttcc tcgatgattt taggatgatc gatgattcgc
    33661 ggatcagcca tcatgattcc tgaaacatcc gtccaattct cataactttc cgcttgcaac
    33721 atcttcgcca gtatcgcgcc ggtaatgtcg cttcccccac ggcccaataa tttttctttt
    33781 cctgcagggt tcgccccgta aaaccctgga acaaccattt tcggatatct tttaaatgct
    33841 tttgtgattt cctgtgctgt tttttcctga tcgattttcc catcatagtc aaagaaaatc
    33901 aggtcttttg cgtcaataaa ctggtaacca agatactcag ccatcagttg agcagtcaga
    33961 tattcacctc tacttactaa ataatcttca gttatctttt cgtcttccag ttgtttctgt
    34021 atgcgtgcta gttcatcttc aataggtaac tgcagggcaa gaacatctct tacttgaatg
    34081 aaacgatcac aaatcctttt ccaaaggggt gtccaatcaa catgatgctg aaggtaagca
    34141 taaatcaaat aaagtaagtc tgttactttg tcatcatctg acgtacgttt gcctaaagca
    34201 gaaacaacga tcacttggcg attgggatct gaaagaacaa ttgtacggac ttttttgaat
    34261 tgctcttcat gagacatcga tgttccgccg aatttcgcta ctttcagcgt attactcatc
    34321 ttgccacctc cgctattttc tgcattaaag ctgcagcaat ctgtacggca ttcgcagcgg
    34381 ctcctttgcg tatgttatcg gcagtcgtat agaacaacaa tccgttctct aaagaagtgt
    34441 cttttctgat cctcccaaca taaaccatat ctgttcctct agcaagaatg ctcgtcggat
    34501 atttatgtgc tgctagatca tcttctaatt gaatccctgg aaactggctg agacactctc
    34561 taatttcttc cgtcgtaaac tctttttgca gttgaactgc cacagagact ccatgtccat
    34621 tttcgattgg cacacgtacg caggttgctg atactggcaa agcaggctga tgcagaatct
    34681 tacgggtctc atttatcatt ttctgttctt cttttgtata cccattttcc aaaggctgat
    34741 cgatttccgg aatcaccgtt tggctaatat tataaggata caaaactggt tcctcgtcat
    34801 tttgcgtacg atgaagatct tcaatgcctt tttgcccgga accagaaact gcttgatagg
    34861 tcgaataatt gactcgctgg ataccaaagg cttcttgtaa agctttaagc ggaagtaccg
    34921 cttgaatggt tgaacaattt ggatttgcaa tcagccgatt caatgtatag tcttccagat
    34981 tgacctctgg aacgattaat ggaatatccg aatcttcccg ccatgctgag gaattatcta
    35041 tgacaaatag tccctgactt cgtgcgattg gcgcaaactc tttggataca gaagcacctg
    35101 cagaaaatag cgctaaatca tatccttcaa atgaagtttc tgtcaattcc tgtacagtgt
    35161 gtttcttttt cttgaataca caaactttac cggctgaacg ttttgaagct aataatttca
    35221 atcgtcctac tggaaaatca tattcttcta acactttgag catcgtttgg ccgactaatc
    35281 cggttgcccc cacaatacag gtatctattt cgcgcatttt tctccatctc ctctataatt
    35341 cgcgtaacgc agcaatcaat gctgtatttt cttttgtctg atcatcactt atttttaata
    35401 cacgtgcagg tattccaccg acaacagttt ctgctgccac atcttctaaa accactgctc
    35461 ctgctgccac gactgcatct tttccgatat ggacaccttc aacaatcact gcattcgcac
    35521 ctaccagtac gccgtcttct acgataaccg gtttggccga tgctggttcg atcacacctg
    35581 ctaagacagc tcctgcacca atatggcagt ttttgcccac tgtcgctcgt ccacctaaga
    35641 cagcacccat atcaatcatc gtgttttcac cgattactgc accaatattg ataatcgctc
    35701 ccatcatgat cacggcattg ttgccgattg acacttgatc tctgataata gcacctggtt
    35761 cgatacgagc attaattttt ttaatatcta gcaaaggaac tgcgctgtat cttgcagcag
    35821 tttcgatttc gatttcaatg attttttctg attcagcttc taaaaccgga gcaatcgtct
    35881 catagtcacc aaaaacaacg aagctgcttc cttcaccaaa aactttactt tctttaaatt
    35941 cgattggctc ggttacattc agatagactt tcacaggcgt ctttttagat gccgtttgaa
    36001 tgtagcggat gatttcttga gcttccacgt tttttacacc cctttgtcca ttaataaatc
    36061 ttctaatttg taataacctg gtgactgatc tttcaaccag tccgctgctt ctactgcgcc
    36121 tgcagcaaag attcgattag aaaaagattc atgtttgatc gtaaatattt catcagtagc
    36181 agcaaacaat acatcatgtt caccaggtaa agaacctgca cgaatactgt gtacaccaat
    36241 ttggttttct tctctttttt tcccttgcca gttgtatacg ggctgcagct tttgcacttc
    36301 ttggattgtt tgaagcagca tatttgcagt accagatggt gcatcttttt tacgactatg
    36361 atgtttttca atcagttcga tctgccagcc ttgcaacatt gcagctgctt gcttgattag
    36421 ttgattcatc accatgatgc cgaacgaaaa attagcacta tacatcacag gtacatgttt
    36481 ggcatgttct tttatttctt ggaactgtga ttgtgtataa cctgtcgtgc caatgactaa
    36541 cggaagttga tatgataagc tatacgaaag gattttctct aaattatctg gatgagaaaa
    36601 atcaataagc agttctggca gttccgtaaa agcaagcgta gttgcttctt taggtgccgt
    36661 ccgtaaaaga aggaccgtat ctcctctttc ttgtgcaact tcttcgactc tatttcccat
    36721 ctgtccactg ccaatcagtc cgattttcat ttttcaaccg tcctttcttt taacaaaagc
    36781 tgagctaatt gtttcttttt ctcttttgaa agagaaacta aaggcaggcg tagaggtccg
    36841 gaaggtaagt ctaataatga catggctgct ttgacaggaa taggattggt ctcactgaac
    36901 aaggcatgga tcagttcaag tgcatttaat tgcttttctt ttgcttgctg ccatcttccg
    36961 tcctgcgtat cttttactaa ttcatggact attttaggtt ggatattcgc ccatacactt
    37021 attacaccgc ttcctcctac actcattact ggtaggatca aatcatcatt tccactgtaa
    37081 aggaagaatt cttctccaat caaccgagcg gcatccatca cataagccat atctcctgag
    37141 gcttctttca aaccaatgat attaggatgt tctgctaaat ttactaatac atggattgga
    37201 atcgacatgc ctgttctaga aggaacatta tacaaaataa gcggaatcgg tgaggcttct
    37261 gcaattgctg taaaatgagc agctagtcct gcatcacttg ttttgttgta gtaaggggtg
    37321 atgaccaaaa gtgcgtctgc ccccaaagaa gcaaaatgct tcgctttttc aatcgtttct
    37381 ttcgtattat tcgttccagc tcctgcaatc accggcacac ggccagccac tttctttact
    37441 gtcagttgaa ggatctgctc ctcctcagat tgtgtgagcg tagaagattc tcctgttgta
    37501 cctaaaacta ataatccatc tgtttgttca tctaaatgga attggatcaa tttttccaaa
    37561 ccagcataat ctactgatcc ctcttcattc atcggcgtga tcagcgccac gatcgaacct
    37621 tttatcatct tcattcctcc aatattcttg tccattcatg aactgctggg ccagccattt
    37681 gtatcgcatc ctcaccagag atcatcaagt ctccttgttc caagtgaaca actacctgat
    37741 ctgctgaaat tttcccagtt tccttagcaa ccacataaga ggcacagcaa cctgtaccgc
    37801 aagcaagtgt ccaacctact cctctttcat aggtacggac gatcaattct tgttcattga
    37861 ctacttctac aaagttgaca ttggtttttt ctttgaacaa aggatgatga cagatcagtt
    37921 cacctctttt tctccagact tcctcacgcg cattttcaac aaaaacaacg gtatgaatcg
    37981 ttcctaaaaa taaactagtg atcgttactt tttcaccatt gatctctatt tcctggtttt
    38041 ttattggata agtttgatta gctttgattt tttccggaga atcatctggc actcccatat
    38101 ctacgctgca ataaaaaggt gagggatcca acaccgtaat ttttcttgtg cctgctaatg
    38161 tctctacgtt gaattcctgt tcattcatca tgttctgatc atttacgtat cgagcaaaac
    38221 agcgaatgcc atttccgcac atcggcgcgc ggctgccatc tttgttgtaa tagatcattt
    38281 ccaacggatc gatttttacg gcgatcaatc cgtctgtatc atatttttct tttgtgcata
    38341 aagaagcggc taattgacta taatctgtgt cttctgcgta atctaataag ataaaatcat
    38401 ttccgcagcc attatattta tgaaggatca taggaaactt ccccccttag tagatcagaa
    38461 aatgcttgtc tgcgaacaat cagtcttgat tggccattat tgacaaaaac aactgctggt
    38521 cgtgtcgctc gattgtagtt gctgctcatc gagtatccat aagctcccgt ggaataaaca
    38581 gctaaaagat cacccgattc tgctttcggc aaataagctt ctttaattac cacatcgcct
    38641 gattcacaca tttttcctgc aatcgtcact ttctctgttt tttcttccga cagttttgct
    38701 gctaaatcac aatcgtattc tgcctgatac agagctggac ggatattatc tgtcatcccg
    38761 ccatctacaa agtaatattt tttatgtggt gtctttttga tgaaaccaat cgtgtatagt
    38821 gttgtaccag cttctccaac aatacttcta cccggttcga tcagtaattg acgaatggtc
    38881 agttttcgtt tttccaaagc ttcttccgta tattcaatca gtttttttag tgcctcttcg
    38941 actgggatcg gctgatcatt ttgtgtataa cgaatcccaa accctccgcc aagattcaag
    39001 gaaagaggtt ggtcaaaatc ggttaagtaa ttcgtcaatt tgtcgatttc agctaaccaa
    39061 gcagtaacat caaaaatttg tgacccgata tgagcatgaa atccttctaa ctctagatag
    39121 ctgctttctt ttatcaactg aagggcttgt tggcactctt tactttcata gctcatgccg
    39181 aattttgaat caatatgcgc agtgacgata tattcatgag tatgtgcttc tactccgaca
    39241 ttcaaccgaa gcatgataca gatttttttt aaatagcgtt ggcttaattc tgctaataat
    39301 tcaacttcca tcaaattatc ggcaacaata tgcaacaaat cattttcaag tgcatagatc
    39361 agttcctctg gcgtcttgtt gttaccatgg aagtagatag tatctctagg aaaatcagct
    39421 tgcaaagcgg tatagatttc cccaccactg acaacatcta gcccgaatcc atattcacga
    39481 actaaatgca gcatctcaat cgtttggaat gctttagagg cgtaaagcac ttttgtatca
    39541 aaacgtttcg atacaaagcc tcgcttgaat gacagcatcg tttgtctcat cttttcttca
    39601 tcatacacat acagaggtgt cttatacttt tctgccaatg tgcttgctgt gactccgcca
    39661 attatcaatt ctgtcatttt ttcattcctc ttttcacttt ttttccgttt ttattggaat
    39721 ttcatttgat aaagatttga attttattgg aataatatta aacaaaattt tctgaattgt
    39781 ctagtatatt tcgaaatatt tttttaaaaa taagaataaa aatggttata aaaacaaaat
    39841 acccgaacta ttagaagcta tttatacata aaaacataaa ttcaacacta tttagtgatg
    39901 aaaaaatatt ttcgcttcaa tatagttcgg aatagttcgg atattttcaa ttttatgaat
    39961 catttcaatt tcttcataaa taagtgcagt ttcttctttc tctagctttt gccattcatc
    40021 tttcaaaata taaataaaat caaatacttt tttatttttc aatttgctga ttccacaaaa
    40081 aagataaagt ccttataatt gtgtaaaagt aaaaaggcca t
//
LOCUS       DACEDM010000018        40078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEDM010000018 DACEDM010000000
VERSION     DACEDM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.018"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            492..1199
                     /locus_tag="IWO50_000867"
     CDS             492..1199
                     /locus_tag="IWO50_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0358006.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1258..1902)
                     /locus_tag="IWO50_000868"
     CDS             complement(1258..1902)
                     /locus_tag="IWO50_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0358007.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2029..2316
                     /locus_tag="IWO50_000869"
     CDS             2029..2316
                     /locus_tag="IWO50_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358008.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2424..2744
                     /locus_tag="IWO50_000870"
     CDS             2424..2744
                     /locus_tag="IWO50_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0358009.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2825..4138
                     /locus_tag="IWO50_000871"
     CDS             2825..4138
                     /locus_tag="IWO50_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0358010.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            4135..4854
                     /locus_tag="IWO50_000872"
     CDS             4135..4854
                     /locus_tag="IWO50_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0358011.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4982..5533
                     /gene="raiA"
                     /locus_tag="IWO50_000873"
     CDS             4982..5533
                     /gene="raiA"
                     /locus_tag="IWO50_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0358012.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5755..8289
                     /gene="secA"
                     /locus_tag="IWO50_000874"
     CDS             5755..8289
                     /gene="secA"
                     /locus_tag="IWO50_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0358013.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8415..9516
                     /gene="prfB"
                     /locus_tag="IWO50_000875"
     CDS             join(8415..8477,8479..9516)
                     /gene="prfB"
                     /locus_tag="IWO50_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0358014.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9621..10307
                     /gene="ftsE"
                     /locus_tag="IWO50_000876"
     CDS             9621..10307
                     /gene="ftsE"
                     /locus_tag="IWO50_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0358015.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10291..11184
                     /locus_tag="IWO50_000877"
     CDS             10291..11184
                     /locus_tag="IWO50_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358016.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11336..12784
                     /gene="cls"
                     /locus_tag="IWO50_000878"
     CDS             11336..12784
                     /gene="cls"
                     /locus_tag="IWO50_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0358017.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13121..13978
                     /locus_tag="IWO50_000879"
     CDS             13121..13978
                     /locus_tag="IWO50_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0358018.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14093..15013
                     /gene="pstC"
                     /locus_tag="IWO50_000880"
     CDS             14093..15013
                     /gene="pstC"
                     /locus_tag="IWO50_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0358019.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15013..15897
                     /gene="pstA"
                     /locus_tag="IWO50_000881"
     CDS             15013..15897
                     /gene="pstA"
                     /locus_tag="IWO50_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0358020.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15912..16718
                     /gene="pstB"
                     /locus_tag="IWO50_000882"
     CDS             15912..16718
                     /gene="pstB"
                     /locus_tag="IWO50_000882"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358021.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16734..17492
                     /locus_tag="IWO50_000883"
     CDS             16734..17492
                     /locus_tag="IWO50_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358022.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17505..18182
                     /gene="phoU"
                     /locus_tag="IWO50_000884"
     CDS             17505..18182
                     /gene="phoU"
                     /locus_tag="IWO50_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0358023.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18433..19451
                     /locus_tag="IWO50_000885"
                     /pseudo
     CDS             18433..19451
                     /locus_tag="IWO50_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            19448..20215
                     /locus_tag="IWO50_000886"
     CDS             19448..20215
                     /locus_tag="IWO50_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0358024.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20315..21883
                     /gene="liaX"
                     /locus_tag="IWO50_000887"
     CDS             20315..21883
                     /gene="liaX"
                     /locus_tag="IWO50_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0358025.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            22183..22500
                     /locus_tag="IWO50_000888"
     CDS             22183..22500
                     /locus_tag="IWO50_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0358026.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22500..22850
                     /locus_tag="IWO50_000889"
     CDS             22500..22850
                     /locus_tag="IWO50_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0358027.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23017..23955
                     /locus_tag="IWO50_000890"
     CDS             23017..23955
                     /locus_tag="IWO50_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0358028.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23970..24800
                     /locus_tag="IWO50_000891"
     CDS             23970..24800
                     /locus_tag="IWO50_000891"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0358029.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24844..25869
                     /locus_tag="IWO50_000892"
     CDS             24844..25869
                     /locus_tag="IWO50_000892"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0358030.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25884..26822
                     /gene="galU"
                     /locus_tag="IWO50_000893"
     CDS             25884..26822
                     /gene="galU"
                     /locus_tag="IWO50_000893"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0358031.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26947..27387
                     /locus_tag="IWO50_000894"
     CDS             26947..27387
                     /locus_tag="IWO50_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0358032.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27390..27992
                     /locus_tag="IWO50_000895"
     CDS             27390..27992
                     /locus_tag="IWO50_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0358033.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(28051..29157)
                     /locus_tag="IWO50_000896"
     CDS             complement(28051..29157)
                     /locus_tag="IWO50_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0358034.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            29367..30368
                     /gene="ccpA"
                     /locus_tag="IWO50_000897"
     CDS             29367..30368
                     /gene="ccpA"
                     /locus_tag="IWO50_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0358035.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(30705..33050)
                     /locus_tag="IWO50_000898"
     CDS             complement(30705..33050)
                     /locus_tag="IWO50_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0358036.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            33329..34231
                     /locus_tag="IWO50_000899"
     CDS             33329..34231
                     /locus_tag="IWO50_000899"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0358037.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            34444..36219
                     /locus_tag="IWO50_000900"
     CDS             34444..36219
                     /locus_tag="IWO50_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0358038.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(36285..37871)
                     /locus_tag="IWO50_000901"
     CDS             complement(36285..37871)
                     /locus_tag="IWO50_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0358039.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            38122..38346
                     /locus_tag="IWO50_000902"
     CDS             38122..38346
                     /locus_tag="IWO50_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0358040.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            38488..>40078
                     /locus_tag="IWO50_000903"
     CDS             38488..>40078
                     /locus_tag="IWO50_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0358041.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
BASE COUNT        12991 a         7072 c         8484 g        11531 t
ORIGIN      
        1 ttagttcgag agctcttttt tttgctttgc taccttgttc cttgaagatt ctgctgaatt
       61 aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg aaagaaaaag
      121 tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac aatattgaag
      181 tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc ccaacgaatc
      241 atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat aagtcggcat
      301 tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa aaaattgttt
      361 ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg gatagaaacc
      421 tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa acgaaaggac
      481 gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac caattaaaaa
      541 atgaaatcga acagggtata tggaaagtcg gagaccgtct tccttcagaa agagaattag
      601 cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac ttggcagatg
      661 aaggaatctt agaaagaaag atcggttcgg gtacgtatgt tgcgcgagaa aaagtacaag
      721 aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat cgagtaccgt
      781 ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg gaaaaactaa
      841 acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca gatgatgtcc
      901 ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac agcaaagcag
      961 agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag atcggtccag
     1021 cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat ttagaggtaa
     1081 aacgaggaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac ggtacgccgt
     1141 ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg gaaaaataaa
     1201 aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca cctgttttta
     1261 tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa attggacttg
     1321 tccgttcagc agctcaatga tggattcttt aaagtggtca gcactttttt catctacagc
     1381 acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat gttggagttc
     1441 aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga tctcctgttg
     1501 caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat gagtgtatgc
     1561 tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga ctactgcagc
     1621 tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg ccgttccact
     1681 tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga tgaaggcgct
     1741 gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaac tttttgcttg
     1801 gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga tctcaatctc
     1861 actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac ctcttttttt
     1921 cattttcttt attcagtata aataaagttc taggttgttt caacactgta acggttgcgc
     1981 aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat gcaggaacga
     2041 atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc tcaagtaacg
     2101 ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc caaaacagca
     2161 agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg tgcctatggc
     2221 tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata tgtcgtgttc
     2281 aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta gaaaacgttg
     2341 acaaatttct ttctttttat ataatagagt aacagacgcg aagaaaacgt ttgcatagtt
     2401 tgttttaaaa aggagagaaa agtatggcta aaaaaacaat catgcttgta tgttcggctg
     2461 gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca aaaggaatcg
     2521 actcggatat tttcgctgtg tccgcttctg acgcagataa caatcttgca aataaagacg
     2581 tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt gagaaacgtc
     2641 tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt atgatgaatg
     2701 gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga ataaatagaa cagtataact
     2761 atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt ttagggcgaa
     2821 gaatatgaat catttttggg gtagaaggct tctgacgaga gatattgaag caatagactg
     2881 tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt tgggcaactg
     2941 gattctttgt catcgctgtg gaatgaggac gtccgtcatt gatggacagc ttgcagatta
     3001 cggttatttt tgtacacatt gtctatattt agggcgatgc gacactcaac aagatttata
     3061 tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga caggacagct
     3121 tactgaaaaa caaacggaaa tctcaaagag aatactttgc cattcagaaa aaagacatca
     3181 tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta ttttagtgga
     3241 aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg tttgtaatga
     3301 attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc tccatgggaa
     3361 tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc agctataccg
     3421 tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc cttataacgg
     3481 ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa ccagacggac gattcatcta
     3541 cttaagtgcc acacctgata aaaagttact aaaggaaatc aagcagacgt ttgacatcca
     3601 caaacttgct ttacgctttc atcggcgttt gcttcctgaa cccgtcttgg ttttcatgaa
     3661 taattggcga caaaaatgca agaacaagaa gaagataaaa tctttggtgc ggatcattca
     3721 tcaattaatt aaagaaaacc atatcctttt attttgtcca agtatcacga tgatgcatcg
     3781 tttgaaagcc acacttcaag aggctcttcc gagatatgag atcacagatg tttcttcaag
     3841 agacaatcag cgggcggaaa aagtccagaa aatgcgagaa gcaaaatatg acatactgtt
     3901 aaccacgatg atccttgaac gtggagtcac atttgaaaga atatccgtta tcgttttagg
     3961 agcggatcat ccggttttta ctaaatcttc tttggtacaa atcgcaggaa gagccgatcg
     4021 taaagggcct tatacgaaca gtcaagtata ctttttctat gaagaaaaaa ctgcagctat
     4081 tcgaaaagtt tgtcaggaaa tcaaagaaat gaatgaagaa gggaagaaga tgctgtgagg
     4141 tgcagttgct gcagtaaaga tatcttccaa aatttaacat tcagcgagat tattggtgtt
     4201 tcgaaataca gattacctca gacttgttca gtttgtcgcg cacaatttca tttattggaa
     4261 acaagaggct gttctggctg tcaaaaacca atccatttca atctagagag tacgaaaaag
     4321 aaagtaaagt ctattcccgt ttactgcagt gactgtcaag aatggaagaa gaaatatccg
     4381 acctatgatt ttcagcaccg tgcctttttt gtgtacgatg aaaatatgaa ggcctggctc
     4441 aatcaataca agtttatggg agatatccga ctagcaggca cctttgcata tagatggacg
     4501 cagataaaaa aagaataccc gacatacatc tattgtccaa tacctttatc tgaaaaaagg
     4561 cgaacagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaag aattcctttt
     4621 cagatgctac tcaaaagaga gaagcatctg ctggctcaag caaaaaacac gcgaaaggaa
     4681 agattggaga tgcctcaacc ttttgaactt gcagtcacct tttctaagat acaaaataaa
     4741 aaaatattga ttattgatga tgtctataca acaggacaga ctcttttcca cgcagcagat
     4801 tgtttgcagg tcgcacagcc gaaaatcatc cgaaccattt ctttggcgag atgaattaga
     4861 tgtggaaatt tgatagcgat ttaatttgca aataacctag agttcgatat aatagaatta
     4921 agaagaggga aagagtggtc cttctccttc ttgattgtgg tagacgaaag gggtaatttt
     4981 tatgtttaga tataatgtac gcggcgaaaa tatcgaagta actgaagcga ttcgcgacta
     5041 cgttgagaaa aaagtaggca aactagaacg ctatttcagt gacgcacccg atgcaacggc
     5101 gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa cgatcccatt
     5161 accttatctt gtattacgtg ccgaagaaac ttctccagat ttatacgcaa gtatcgattt
     5221 ggttgttgat aaattggaac gccaaatccg caaattcaaa acaaaaatta atcgtaaatc
     5281 ccgtgaaacg ggcatgaata ctgcagatgc tgctgcttta ttcaatgaag aggacgaaaa
     5341 cgaaaatgat tctgaattag atatcgtacg tacgaaacgc ctttcattga aaccaatgga
     5401 tagtgaagaa gcagtcttac aaatgaacat gctgggacat aacttcttta tttttgaaga
     5461 tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat acggactgat
     5521 tgaaacagac tgatcagcct tacttaaata acaacgaaaa cttgcagcag ctactgacag
     5581 ctgccgcaag tttttttgag tttcctcctc ttttagatga aatatgaata aattatgaag
     5641 ttcttacttt aatacgctta agatatttca attatctata actaatgata aaatgaaaag
     5701 taaagtctac atagtctgga taatacacag aaggaaaagg agattacaca acgcatggcc
     5761 aatttgattc gttcacttat cgaaaacgat aagaaagaac taaaacgttt agatagcatt
     5821 gcaaaaaaag ttgaagcata cgctgaccaa atggcagcat taactgatga acaattacag
     5881 gcaaaaactg ctgaattcaa gggcagatac caaaaaggag aaacattaga tcaattactt
     5941 ccggaagctt ttgcagtcgt ccgagaagca gcaaaacgtg tattaggttt atatccatac
     6001 cacgtacaat tgatgggtgg aatcgtgctt cacgatggaa atatccctga aatgcgtact
     6061 ggtgaaggta aaaccttgac cgcaacgatg ccagtatatc taaatgctct gactggcgaa
     6121 ggggtacacg tagttaccgt caatgaatat ttagcaaccc gtgactcgac ggaaatgggc
     6181 gaactttata atttcttagg attaacagtg gggttgaata tcaactcaaa atcttcggat
     6241 gaaaaacgcg aggcttataa ttgtgatatc acttatagta cgaataatga attaggattt
     6301 gactacttga gagacaatat ggtcgtttac cgccatcaaa tggtacaacg tccgctgaat
     6361 tatgccattg tcgatgaagt tgactcaatc ttgattgatg aagcgagaac gccgctgatc
     6421 atttcagggc aagcagaaaa atccacagct ttatatacaa gagcagacaa cttcgtgaaa
     6481 cgtttgaaag aagaagaaga ttataaaatc gatgtacaat caaaaacaat cagtttaacc
     6541 gaagcaggga tcgaaaaagc agaagaaaca ttcggtttgg ataacttata cgacattgaa
     6601 aatactgcat tgacccatca cttggatcaa gcattgagag ccaactatat catgctttta
     6661 gatatcgatt atgttgtcca agacggaaaa gtactgattg ttgaccagtt cactggtcgg
     6721 atcatggatg gacgccgtta ttcagatgga cttcaccaag caatcgaagc taaagaaggc
     6781 gtagagatcg aagacgaaac gaaaaccatg gcgacgatca ctttccagaa ctatttccgt
     6841 atgtacaaga aacttgcggg aatgactggt acagctaaaa cagaagaaga agaattcaga
     6901 gaaatttata acatccaagt ataccaaatt ccgacaaatc gtccggttat ccgtgatgat
     6961 cgtccagatt tgctttaccc aacattacaa agcaagttcc acgctgtcgt gcaagatatc
     7021 aaagatcgct accgcaaagg tcaacctgtg ttagtcggaa ctgttgcggt tgaaacatct
     7081 gagttattat cagatatgct gaatcaagaa cgtattcctc atgaagtgct gaatgcgaaa
     7141 aatcacttta aagaagcaga gatcatcatg aatgctggtc aaaaaggtgc tgtgacgatt
     7201 gctaccaaca tggctggtcg gggaacagat atcaagttag gacttggggt aagagaactt
     7261 ggtggtctag cagttatcgg gacagaacga catgaatctc gtcgtatcga taaccagctt
     7321 cgaggacgtg caggacgtca aggagaccct ggtatgtcac agttctactt gtcattggaa
     7381 gatgatttga tgaaacgatt cggttcagaa cgaatcaaag ctttcttaga tcggatgaag
     7441 atcggtgagg aagatgctgt tatccaaagc aagatgctga cgaaacaagt agaatctgca
     7501 caaaaacgtg tagaaggaaa taactatgac acacgtaaaa acgtcttaca atacgatgat
     7561 gtgatgcgtg aacaacgtga agtaatctat aaacagcgac aagaagtaat catggaagaa
     7621 aaaagcttat ccaagccact aatgaacatg gtcaaacgga caatcagtcg tgtagtagat
     7681 gcgcatacac aattagagaa agaaaattgg aatttggaaa gtatcatcga ttttgccggc
     7741 agtactcttg tccatgaaga tagcattact cttgcagata tcgaaggaaa aacagctcag
     7801 gagatcaaag agtatctaaa cgaacgagca caagaagtat ttaataccaa agcggctcaa
     7861 ctaaatggtc cagagcaact gttggaattt gaaaaagtcg tgatcttgcg tgttgtggac
     7921 tctaaatgga cagaccatat tgatgcaatg gatcaattac gtcagtcaat cggcttacgt
     7981 gcttatggac agaacaatcc attagtcgaa tatcaaacag aaggctataa gatgtttgaa
     8041 gatatggtag gcgccatcga gtatgaagtc actcgaatct ttatgaaagc tgagatccgt
     8101 caaaacgtac agcgtgaaca ggtttctcaa ggaacagctt ctcactcaga agacggagat
     8161 gccaaagaaa atagtaatac tagcgcgaaa aaacagccaa tccatgtgaa taaggtcgga
     8221 agaaatgatc cttgtccttg tggaagcggg aaaaaatata agaactgtca tggaaaaaat
     8281 gcaaactaat ttctgacagc aggattggtg agacaaattc tttttttaag aattcgtctc
     8341 accttctttc tgttatttgc tgattctgtt agactagata taatgaaaaa agtatagtga
     8401 aagaaggaaa aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa
     8461 aggtcacaag tttcaggagg tctctttgac ttagatcagc tggaagaaga catcgcggaa
     8521 gcagaagcaa aaatggctga acctggtttt tgggatgaca gcgaagccgc acaaaaagtg
     8581 atcagcgaaa ataacgctaa taaagaaacc tatgaccaat tccattctct tgccgaagaa
     8641 ctagaagaaa tcgaattgat ggtggagatg ctgcaagaag aacctgatgc agcaatgcaa
     8701 gaagaagcag aaacacgtat caatgcatta gatgaaaaaa tgaagacgta tgaattatct
     8761 atgctgttag acggacctta tgatcgaaac aatgcgatcg tcgaattgca tccaggggct
     8821 ggcggaacgg agtcccaaga ctggggcagc atgcttttac gtatgtatac acgttgggca
     8881 gagcagcatg gctatcaagt agaaacacta gattatcaag caggtgatga agcagggatc
     8941 aagagtgtga ccctgctgat taaaggacat aacgcttttg gttatctaaa atcagaaaaa
     9001 ggtgttcatc gccttgtacg gatttcgcct tttgactcag cgaaacgacg tcatacttct
     9061 ttttgttcgg tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca
     9121 gatgatttga aaatcgatac gtaccgagca agtggtgcag gtggtcagca catcaataaa
     9181 acagaatctg ctgtacggat cactcacttg cctacaggaa cagtagtagc tagtcaggca
     9241 caacgctcac aattgaaaaa ccgtgagcaa gcgatgggga tgttaaaagc taagctttat
     9301 caattagaag tggaaaagaa agaacaagaa gcagctgcac ttcgaggaga acaaaaagaa
     9361 atcggctggg gttcacagat ccgttcttat gttttccatc cctattctat ggtaaaagac
     9421 catcggacaa acgtagagac aggaaatgtc caagcagtta tggacggtga ccttgattta
     9481 tttattgacg gctatttaaa aatgcatcta caataagaac ccattgttaa attgaaataa
     9541 aattgtaaga ttttgcaagc atgttgaaac attgccatgc tataatagct aaagactgaa
     9601 aaagatatgg agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaatg
     9661 gtacgaccgc tatccgcaat ctctcgattg tcatcgaaca aggagagttt gtctatgtgg
     9721 ttggtccgtc tggagccggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag
     9781 cttcaaaagg aactttgaac gtcgcaggtc atgatctaat agcaatcaaa aaccgcgaag
     9841 ttccttatct acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa
     9901 aaacagtata tgaaaatgtt gcgtacgcta tgcaagttat cggcagaaaa cctcgagata
     9961 tcaaaaaacg agtaatggaa gtgctggacc ttgttggttt gaaacacaaa gttcgagtct
    10021 ttcctagcga attatccggt ggggaacagc aacgtgtctc tattgctcgg gcaatcgtga
    10081 atacgcctaa agtacttatc gcggatgaac cgacaggaaa tctggatcca gaaaactcat
    10141 gggaaatcat gaaattatta gatcggatca atgcccaagg aactactgtc gtgatggcta
    10201 cacataacag tacgatcgta aatacgattc ggcaccgagt aattgctatc gaaaacggcc
    10261 gcattattcg agatcaagcg gaaggggaat atggatacga tgattagaac ctttttccgc
    10321 catttattag aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt tgcttcagtc
    10381 agtgcggtaa cgatcacgtt gactttagtc ggtgtattca tggcagttat catgaatgca
    10441 actaaattag ctcaggatat agaaggaaac gttgatgtat ctgtattcgt agatatcggt
    10501 acaaaagacg aagagatgaa aaagctagaa aaagaattga atgacttaga tcatgttaaa
    10561 aaagttgaat tttcaagcaa agaacaggaa ttgaaaaaaa tccaaaacga aatgggtgat
    10621 tcctggaaat tgtttgaagg tgacaataac ccgttatatg atgtgtatgt agtaagtgct
    10681 actgatcctt cttatacaaa acaagtagca gaagaagcag cagatttgaa gaatgtttct
    10741 cgttctgatt acggtggagc ttcctcagat cggattttcc aaatcgctgg tgcagttcgg
    10801 acatggggat tagttgcagc ggcattgctg ctgttcgtag cgatgtttct catttcgaac
    10861 acgattcgga tcacgatttt gtctagacag cgtgaaattc aaatcatgcg tttagtcggt
    10921 gcaaaaaatg gatatattcg ctggccgttt ttccttgaag gtggctggat cggtctgatc
    10981 ggtgcgattc ttccgatttt gatcatcaca tttggttatg catggttttt ccaattgatc
    11041 aatccatcat tattacgttc ccattattca ttgattcatc cacaaaatat cgtttggaaa
    11101 atcaatcttt taatggtcgg cattggtgtg atcattggtt cacttggctc gatcatttct
    11161 atgagacgtt tcttgaaatt ctaataccac aatataaata aaatgagaaa gttggacagt
    11221 ttcgttccag ctttcttttt ttattggcta ggagaataga aggaagcggc ttttttccct
    11281 ttactttaaa aaacaacttg ggtattctta agttggtaga agaagaggtg aagccgtgaa
    11341 aattatcgtt gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat
    11401 catcgttttt cgagaaagaa aggaaactgc tcaaacatgg gcatggctgc tcgttttgat
    11461 gttcattcca gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga
    11521 gaaaatcttt gatttgaaga tccaagggaa aatcggtaag aacctggaaa ttgaagaaga
    11581 caaacaggca ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa
    11641 acaattgatc tatatgctca cggtatttga aagtacgctc tatacgacta aaaatgaaat
    11701 cactttattt acagatggtc gtgaaaaatt cgatgcgctg attgaagata tcaagcaggc
    11761 agaagaccat atccacttcc agtattatat ttatcgcagt gatgcattag gtgaagaagt
    11821 tcgagatgca ttgacagagg cggcaagacg aggggtaaaa gtcagagtcc ttcttgatgc
    11881 atggggttcc acacaagttt cttccagctt ttttcagaat ttgaaaaaag caggcggaga
    11941 agtcgcattt ttctttcctc tatttgttcc gtatattaat cctcggatca attatcgcaa
    12001 tcatcgtaaa atcgtagtta ttgatgggaa aatcggatat acaggtgggt tcaatgttgg
    12061 aaatgaatat ctaggattgg taaagaaatt tggttattgg cgagacaatc atttgcgtat
    12121 ttacggagaa gcggtgtata ccttgcaaaa ccgttttttg atggactgga attctcagca
    12181 tacaaaagaa gtaggctatt ctcccaaatt ttttccgtcc attcacacta ctggtgatat
    12241 agctgttcaa atcgtgacaa gcggaccaga taccgaacat gaacaaatca aaatgactta
    12301 tttgaaaatg atttcgttag ccaaacggga gatattgatc caaacgcctt attacatacc
    12361 ggatggatcg attcatgaag cactgaagtt ggctcttctt tcaggcgtaa aagttcatat
    12421 ccaaatacct aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc
    12481 agagctgatc gagtatggag caaggatcga aacatatgag aatggattta ttcatgcaaa
    12541 gacgatgatt atcgatggtg gcattgtttc agttggctca gcaaatatcg atgttcgttc
    12601 atttcgcttg gactttgagg tcaatacact ggtttatgat gaacgaatgg cttcacgtgt
    12661 aagaaaagct ttttttgaag actcgaaaat ttctacgcat ctcacaaaag aaatgtatga
    12721 aaaccgagga ataatcatca agatgaaaga aggattagca agattgattt cgccgctcct
    12781 ttaaaacata aaaaggacat gagaaccatc aacagatgga gatctcgtgt ccttttctaa
    12841 cggggccatt tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa
    12901 ctttggggta tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa
    12961 agagacacat ttcaaagcca gtatagcata gaaactttta atgagatagt tttttttaca
    13021 attatgagaa tttattttgt tatttaatga ttttgattcg ttttcatcaa gtaatgaaaa
    13081 tgataaaata aacatacttt aggattaaag gagatttcta atgaaaaaac ttaaattatt
    13141 cttaccaata ataggattag ccattctttt atcaggctgt tcaaagtgga ttgatcgagg
    13201 agaatccatt acggctgttg gctcttctgc tttacagcct ttagtagaaa cagttgctga
    13261 aacttaccag acagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac
    13321 tggtttgtct caagtacagt caggagcagt cgacatcgga aactctgatt tgtttgcgga
    13381 agaaaaatcg gggatcgatg cctcagcatt agtcgatcat cgagtagcag ttgttgggat
    13441 cactcctatt gtgaacaagg atattggcgt aaaagacatc agtatggaaa acctgaaaaa
    13501 actatttaca ggcgagatca aaaactggag tgaagtcggt ggaaaagatc agccagtagt
    13561 tatattgaac cgcgcttcag gaagcggaac ccgtagcacg tttgaaaaat gggtattgga
    13621 cggagaaaca gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccagatcgt
    13681 tgccgacaca cccggtgcaa tcagctatgt tgctttttct tatgtttccg atgatgtaga
    13741 tacgttgaat gttaacggtg tagaaccgac agatgaaaat gtgacaacga acaaatggac
    13801 gatttgggca tatgagcata tgtacacaag agatcatcca aaaaaactga caaaagaatt
    13861 tttagactat atcttatctg atgaagtaca agataacatc gttggagaac taggttatat
    13921 ccctgtttct catatgaaag tcgaacgtga ttggcaagga aatatcgtta aagagtaatc
    13981 tttacacaaa atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt
    14041 tcaatttgta cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga
    14101 tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa atggaacagc gtggaaaatt
    14161 catcagtttt ttatgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt
    14221 agctagtaaa ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt
    14281 tggtacagat tggaatccaa gccaaatcgg agcaaatggg aaaccaatgg taggggcatt
    14341 gcctatgatc acaggttcgt ttattgtgac ctttttatct gctatcgttg ccacgccatt
    14401 tgccattggt gcagcagtat tcatgacgga gatctctcct aaaaacggaa cgaagatctt
    14461 acagccggtc atcgagctgc ttgtaggtat tccttctgtc gtttatggtt ttatcggttt
    14521 atcagttatc gtgccttttg tcagaagtat ttttggcggg acagggtttg gtatcttagc
    14581 tggtacattt gtgttgttcg ttatgatctt gccgactgtc acaacgatga ctgttgatgc
    14641 attaaaggca gtgccacggc actatcggga agcttcatta gcgcttggtg ccacaagatg
    14701 gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc gggattttaa cagcagtcgt
    14761 ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc caaatggtca tcggtaatgc
    14821 agcattgatg ccaacaggac ttactactcc tgcttctaca ttgacgtcca tcttaacaat
    14881 gggaatcgga aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct
    14941 gatcttgcta ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg
    15001 agagctgaaa taatgaacgc aaaacgctca gataaaattg ctacaggtgt tttatatata
    15061 gtttcgggga tcatcgtatt gattttagca gctttactac tttatatctt agttcgggga
    15121 ataccgcata tttcatggtc cttcttgacc gaaccttcca aagcctacca agaaggcggt
    15181 ggaatcggga tccagctatt taactcgatt tatttgcttt tgatcacgat gatcatcagt
    15241 tttcctattt ctttaggtgc gggtatctat ctttcagaat atgctggaaa aaactggcta
    15301 acagatgtga ttcgcacgtc gattgaaatc ttgagctcgt tgccttctgt cgttgtcggt
    15361 ttattcggtt tcttggtttt cgttattcaa ttccagtatg gtttctcgat tatttctggt
    15421 gcattggctt tgactgtatt caacttgcct cttttgacta gaacagttga ggagtcttta
    15481 caagcggtcc attacacaca acgagaagca ggattggcct taggtttgtc acgctgggaa
    15541 acagtgataa aagtggtggt tccagaagcg acaccaggga ttttgactgg tgtgatccta
    15601 agttctggaa gaatttttgg tgaagcagct gcattgatct atacggctgg acaaagtgca
    15661 cctgcgttag attttacgaa ctggaatcca ttaagtgttt caagcccgat cagcattttc
    15721 cgtcaagctg aaacattagc tgtccatatt tggaagatca atacagaagg cacgatgcct
    15781 gatggtgttt ctgtttcggc tggcgcttct gccgtgttga tcattgctgt tttactgttc
    15841 aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga
    15901 gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa tgtcatcacc atgccaaaag
    15961 agaaagaagt agcactatat acggatgatc tacatgtctg gtatggcgac aatgaagcaa
    16021 tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac tgcactgatt ggtccttccg
    16081 gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat gaatgatggg atcgctaata
    16141 cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat taatgcgcct caagtggatg
    16201 tttatgagat gcgcaaacgg attggcatgg tttttcaacg tcctaatccg ttcagcaagt
    16261 cgatttatga caatattaca tttgctttga aacagcacgg cgaaaaaaac aaaaagaaat
    16321 tagatgaaat cgttgaaaca agcttgaaac aagctgcctt gtgggaccaa gtaaaagaca
    16381 gcttggacaa aagtgccttg gctttatctg gcgggcaaca gcaacgttta tgtattgctc
    16441 gtgcgatcgc gatgaaacct gatatcttgc tgctagacga gccggcaagt gctttggacc
    16501 ctatttcgac aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tatacgatca
    16561 tcatcgtgac gcataacatg caacaagcag ctcgtatcag cgactatact gcctttttct
    16621 atctaggaaa agtaatggaa tatgacaaaa ctaacaagat atttaccaga ccaaaaattc
    16681 aggcaaccga agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa
    16741 aagaaattat ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaaag
    16801 ggatcaatct aagtttcaat caaggggaaa tcactgcgat gatcggtcct tctggttgcg
    16861 ggaaatccac ttatctgcgt tctttgaacc ggatgaatga cctgattccg aatgtaacga
    16921 tcaccggaag tgttttatat aaagacaaag acatctatgg tccaaaaaca gatgttgttg
    16981 aactaagaaa agaaatcggc atggtattcc agcagccaaa cccatttcct ttttctatct
    17041 atgaaaatgt catctatgga ttgaaattaa aaggggaaaa agataaaaaa gtccttgatc
    17101 aagtagttga agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa gacaaattgc
    17161 ataaaagtgc tttatcttta tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt
    17221 tggcagttaa tccggaaatc atcttattgg atgaaccaac aagtgcgctt gatcctgttt
    17281 ctactgggaa aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg
    17341 tgacccataa tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg
    17401 gtcacttgat cgaatacaac gatacgaaga agatattcat gaatccagaa aaacaagaga
    17461 cagaagatta tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat
    17521 ttgaagaaga attgctgaat ctccataatc agttctacga aatggggatg atggtgagca
    17581 gcgccgtgca caagtcagtt cgtgcataca tcaaacacga taaaatctta gcccaagaag
    17641 ttatcgaaaa cgatataaat atcaataaca tggaaacacg cttggagaag aaaagttttg
    17701 aaatgatcgc attgcaacag ccagttacaa ctgatttgcg tatgatcatt acggtcatga
    17761 aagctagctc ggatttagaa cgtatggccg atcatgcggt gtctgttgct aaatccacga
    17821 ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg
    17881 attatgtgaa aaaaatggtg gacaacgtcc tagtagctta tgtcaaaaca gacgaaaaag
    17941 acgcacgtac gatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata
    18001 atgcaacaat caaaaatatg caggaaaatc ctgagacagt catcagtgga acagactact
    18061 tgaatgttgc cagttatcta gaacggatcg gagactatgt gacaaacatc tgtgaatgga
    18121 tcgtctactt ggctacaggg aaaatcactg agttaaatac gaaccacaat gagattttct
    18181 aaactgaagc tgtgattatg ccgtttgttt aggaaaagag gttgtgaaat cagcggttgt
    18241 gaaaaagctg ttctgcatca agtaataaga acagtcaaac aaaaaatagc tcctcatatt
    18301 tttcgtttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgggaa
    18361 agggtttgtg acaaaacatg tcacaaaccc ttttcatcat ttcatagaaa ggataaagaa
    18421 tgcaaaaaaa aaattagcat catcactata ttttttattt gtgtgcttac ttcatgggtt
    18481 attcagcatc aagctgcttt atatgaagta cctgtcatcg agatcgaaaa agtacgaata
    18541 aaagaggatc atcaattgat tacaggaaga ctgatgaaca cggatcaaaa aggagaatcg
    18601 atttatttat cggttcctta tcaaaaaaat caaatagaaa cagcaaatat aaaagcgaga
    18661 caaatgtatc tagtccaaat gcatggagat gaatggcagc tagtagcaaa aaaaaatgat
    18721 ggctggcttt tcttcttcac aagtatcttc attggcgcca tgttgcttat tggcggaaaa
    18781 cagggtctat ttgctttagg aagtctcatc atcaatatcg ttttacttta cttttgttgc
    18841 tcatctttac taaaacagaa gggatttctc tattaaatat atctatcctg ttcgtcgtta
    18901 tagggatgat tattagcata ttggctttaa atggatggaa tagaagcagt ataatcaaaa
    18961 tttcagctgc agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc
    19021 attggaaaga ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt
    19081 ctgtttttct ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca
    19141 cagtcattgc gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt
    19201 tatggctttc aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt
    19261 tgttcgtcta tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt
    19321 cgttcaaaga aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca
    19381 gcttaggaat agtattatcg atccctatct cggtcctctt ttttctgact gtaaggaggc
    19441 tgaaacgatg aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat
    19501 tggcggtacg caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt
    19561 cctgttgtta ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt
    19621 tttattgatt gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc
    19681 agcttttgtg agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga
    19741 ttttttagcg atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa
    19801 gactttagcg attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg
    19861 tgctgtttta gatgctagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa
    19921 tccgcaatta tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt
    19981 ggcgtcaaca gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct
    20041 ctggtttatt gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct
    20101 agaatatacg atggctattc ttaccacatt gtctgcacta cttgttcttc ctttaacctg
    20161 cgtgactaca gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt
    20221 acaacttaag tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag
    20281 cttgcaaaca attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga
    20341 tttagtgaaa aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc
    20401 aactgaaaaa gacgaaaagc aaataaaaaa agaagcagac aaggtaactg ctagtcataa
    20461 agaaaaagat caggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc
    20521 tgagccagtt gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt
    20581 agatgaacta gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca
    20641 attcgctgac aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac
    20701 aaaagaagaa ttaggcgagt tgacagaaga agaattagca cagcgtcaga cgttagaagc
    20761 agaaatcaaa gaattagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga
    20821 agcagaatta aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga
    20881 taagtttgaa ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg
    20941 cgaaaaaatg tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac
    21001 tgtttcagaa acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg
    21061 gattgcgaca acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct
    21121 tgatatcaag gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa
    21181 agttgaagct aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc
    21241 tgaacggagc cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg
    21301 tgtgcgtgca gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa
    21361 acttctaaat ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc
    21421 tacgaatggt aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt
    21481 aaacggcaat atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa
    21541 tggtacagtg acatttggtg cgacagcaga aaacctttct gtgtctttgg taaatggtga
    21601 tgtacgtctt acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg
    21661 aaatgtcaaa gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct
    21721 tggaagtatc aacagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac
    21781 aaaccaaatg ttgcaattcc gtcgtgtttc agatggtgaa atcacgcaag tgcaactttc
    21841 aacgacaaca ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga
    21901 tgttcagcct tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat
    21961 tgtgccttat tgccgatagg ctatctcttg aacaccgttt accaagagat tatgaacctg
    22021 ccaattatcg ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata
    22081 tggtaatttg tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa
    22141 cgattttctt catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca
    22201 aaatccaata aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg
    22261 attgatccga ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt
    22321 ccaatcattt tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca
    22381 aactatggac ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa
    22441 caaacaaaaa aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa
    22501 tgtcgtactt tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt
    22561 ttcctgcgat ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt
    22621 ctgtgttgaa tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag
    22681 tcacattggg attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt
    22741 ttgtgggagc agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct
    22801 tgatgtcgct tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg
    22861 tgccgaacta tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg
    22921 gtaattaaat aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat
    22981 gatagagaac actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc
    23041 caattagtga aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt
    23101 gtcatcgtaa ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat
    23161 tattcccata atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg
    23221 atcccagaag agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt
    23281 attatttcaa gaggattacc ggctccagaa gaaatgatcc aggcagcaaa tgaaaatggg
    23341 atagctgtac tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat
    23401 ttagatggtc gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga
    23461 ttaggtgtac tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg
    23521 atcaaacgcg gtcatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa
    23581 ttaacggtga tcggagaacc gcctaaaatc ctggaacatc taattgaaat ccgaggaatc
    23641 gggatcattg atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc
    23701 cagttggctg tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct
    23761 gaagacacga ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa
    23821 acaggacgaa acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca
    23881 atgggctttg acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac
    23941 tcaggcaacg actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg
    24001 agcttgtagg gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg
    24061 ccatgtggct tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag
    24121 attttatgtt gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat
    24181 ttgatcttcc ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct
    24241 tagctattta tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc
    24301 attttatttc tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg
    24361 caatcggtcg ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa
    24421 gcttcttaga aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga
    24481 tctatcgtca accaacattt ctttatgaat cactttggag tttgattgga tttattttgc
    24541 ttttgttttt acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc
    24601 taatgtggta tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact
    24661 ttttaggtgt gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga
    24721 ttctatttat ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa
    24781 aaaatcaaca aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt
    24841 aaaatgaatt atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa
    24901 gtcctcgccg aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa
    24961 gaaatcaaca cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc
    25021 atccaagcat tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc
    25081 attccaacga aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag
    25141 ccagtgatca tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac
    25201 atcttgttag aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca
    25261 agccatgcag aagaagtagc gaaacatgac atcactacga tcacagctgc aagtacagat
    25321 gcaaaagcag caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc
    25381 aatcctgatg tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt
    25441 gctggcgcta ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc
    25501 ggattagctg agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc
    25561 ggattaagcg gagtaggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg
    25621 cgcgcaggca acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga
    25681 atggtcgttg aaggtgtgaa tactacccaa gcagcgatgg aactagccca aataatggaa
    25741 atcgaaatgc ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa
    25801 acagccgcaa aagaaatcat gttacgtgat gggaaaaaag aaaatgaatt ctcattaaca
    25861 attttttgaa ggagcgaatg tatatgaaag tcaaaaaagc agttattcca gcagcaggat
    25921 taggaacaag atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg
    25981 ataaaccaac aatccagttc atcgttgaag aagcgttgaa atcaggaatc gaagacatct
    26041 tgatcgttac aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat
    26101 tagaaacaaa cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga
    26161 cagatgtcaa cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc
    26221 ttcaagcaag agcctttatt ggaaatgaac cgtttgtcgt tatgctaggc gatgatatca
    26281 tggaagatga agtaccattg acacagcaat taatcgatga ttacgaacgt actcatgctt
    26341 ctacgatcgc cgttatgcaa gttcctcatg atgaaacttc aaaatacgga attatcaatc
    26401 caggagaagt attggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc
    26461 caagcaaagc accaagtgat ttagctatta ttggacgtta cttattgact ccagaaattt
    26521 ttgatgtatt gtccgagcaa caaccaggag ccggaaatga aatccaacta actgacgcaa
    26581 tcgatacatt gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttacg
    26641 atgtcggtga caaatttggt tttatgaaaa caagtattga atatggtcta acacatccag
    26701 aagtaggtgc acctttacgt gagtatatcc tgtctttagg taaaacatta gaagcagaag
    26761 gaagcaagat cgatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat
    26821 aacccttttt tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt
    26881 ttatttgttt aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt
    26941 ttgatcatgt caggaggaga aattgcagga ttgattgcag caatcgcctt tgcgatactt
    27001 gtcgttttta tcgttcgtgt gttgttgcaa gtttctaaaa ctttagaaaa agtagatcag
    27061 actgtagcag aagcaaatac gacgatcgaa atcgtcacaa aagacgtaga tcttttgtct
    27121 agacaagtag aaggcttgct ggtcaaaagt aatgaactgc tcttggatat caatggaaaa
    27181 gtcgcaacga tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcagaa
    27241 cttaatcatt ctagtaaaaa catcgcgacg aaagtcggcg gaatcggtaa agcgacagct
    27301 aaagcgacgg ttgccggaaa agtcggtggg acagcgatga aattttttaa aaacaaaaaa
    27361 catactgaaa cgacaggagg aaaataaaaa tggctaaaaa aggtggattt ttattaggtg
    27421 cagtagtcgg aggaacagcc gcagcaatcg ccgcgttatt gttagcacca aaatctggta
    27481 aagaacttcg ggatgaatta gctgcccaag cggatgattt gatggatatg gcttcagatt
    27541 acacagatat ggcgaaagaa aaatcgactg agttaacagg tattgcaaaa gaaaaagcgc
    27601 aagatctttc tcaacaagca ggtgatctag caggtagtgt caaacaaaaa gtaagtgaat
    27661 cagcagatga agcaggaagt gaatctgacg atatcttgtt aaatcttaaa gaacaatcag
    27721 ctgacttatc taatcgtttc aaaaaaacgg cagaagatat gaaaaaacct gcagatgaat
    27781 taggaagtat tgctaaagat acttcagaag atatcatgat tgatgtaaaa gacgctgctg
    27841 gtaaagcagg agatactgtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca
    27901 caaaagaaat gatgaaaatg gcaaaagaag atgcgaaaga agctaaaaaa gaaataaaag
    27961 atacaatgac agattcatct acaaatcact aaaatcacaa atttatagaa taaaaaggtg
    28021 ggagcggact agctctcacc ttttttactc ttatttttct aaaacaagta attcttttgg
    28081 tgtagtcgtg aatggcacac agccatcgct tgtcacatat acacagtctt cgatacgtac
    28141 ccctactttt tcaggaatat agattcctgg ttcaagagag aagcacatcc cttcttcgat
    28201 tactaagtca tttccttcta caagggaagg gaattcgtgg acggttgttc cgatcccgtg
    28261 acctaaacgg tgattgaaat attcgccgta gccatagctg ttgatcacgt ttcgtgcgat
    28321 ttgatcaagt tcacctgctg tcacacctgg acggacggct tcttgtgcgg caagctgtgc
    28381 ttcaagagtg atgttgtata ttttttcttg gaagtcggtt ggtttttgat aagcaattgt
    28441 acgagtagca tcactgcaat agccattcca aacaacgcca agatcgaata acacaagttc
    28501 attcggtgaa atcttcgtgt ttcctggtgt accatgaggg cttgcagcat ttgctcctgc
    28561 aaggactaat gtgtcgaaag acatttgtga aacaccttgt tttttcaatt ggtattcgat
    28621 ttctgcaatg atcgcttgtt cagtcacgcc ttctttcact gctttaaagc caatctcaaa
    28681 tgcaacatcg gcccatttac cagcttcgat cagtttgtct ttttcctcta gagttttgat
    28741 cagttgtaga cgttggatga tggaagttag atcagtgtaa gaatccgttc ccgtcaagat
    28801 atcagagata gcatcgaagc gctcgacagt caaggcgttc ttttcaatcc ctacattgcc
    28861 aggctgacca tagcgagcac gaagttcttt tccgatgatc tcccaagggt tttcgctgtc
    28921 taaatagcca cggacatcat acgcccaact actgttttga gcatcttcaa cttccaaagc
    28981 aggagtgaaa aggaaaggat cttgttcaag agggataaaa agagctagta cacgctcatg
    29041 aggatcactt tcgtatccgg aaaagtaagc gatgtggcca gggtcgctga tataagccaa
    29101 ttcgacttgg tgttgtgcca tccattgacg tagttcattg atttttacgt tattcatttg
    29161 gcaacctact ttctaaatta attttgtatt caataattgt accactgatt tattaaaaga
    29221 ccaacgttct aaaaactgaa aaaagtaaaa attttcatga aaacagagat aaaacggttg
    29281 caattcttga gattatttga tattctattt tagaaatgta agacgtattt tcagaaaaca
    29341 tataaaatat gaataggaga acacaaatgg aaaagcaaac aatcactatc tatgatgtag
    29401 cgagagaggc aaatgtttcg atggctactg tttcacgtgt cgtaaatggc aatccaaacg
    29461 tcaaaccagc aacaagaaaa aaagtcttgg aagttatcga tcgcttggat tatcgtccaa
    29521 acgcagttgc gcgtggcttg gcaagtaaga aaacaacaac agttggtgta atcatccctg
    29581 atgtcagcaa tatgttcttt gcttctttag cacgtggtat cgatgatgtg gcaacaatgt
    29641 acaaatacaa tattatctta gcaaattcag atggcaacga ccaaaaagaa gtcaatgtat
    29701 tgaataactt actggctaag caagtggacg gcgtgatctt catgggtcac catatcacag
    29761 acgaaatccg tggagaattt tcacgttcaa aaactcctgt tgtattagct ggctctattg
    29821 atccagatga acaagtaggt agcgtaaaca ttgattatgt atccgcaaca aaagatgcag
    29881 taaacaaatt ggcaaaaagc ggcaataaaa aaatcgcttt tgtcagtggt gcgttgatcg
    29941 atcccatcaa tggacaaaat cgtctgaaag gctataaaga agcattaagc gaaaataacc
    30001 taccatatag cgaaggattg atctttgaag cacaatacaa cttcaaagat ggtctatctc
    30061 tagtcgatcg gatccataat agcggtgcaa cagctgtcta tgtttcagat gatgaattag
    30121 ccatcggtat tttggatggt ttattagacc gtggcgtgaa agtaccggaa gaattcgaaa
    30181 tcatcacaag taacaattca ctactgacag aagtagctcg tcctcgttta acaagtatca
    30241 cacaaccatt atacgattta ggggcggtat ctatgcgcct attgacgaaa atgatgaata
    30301 aagaagaagt cgaagaaaaa acgattatct tgccttatgg aatcgaagaa aaaggatcta
    30361 caaaataata aaaggacaac cattcagagg ttgtgtgcct gccgtttaac tctgcgttac
    30421 aaggagcaat ttcaaaaaac agcatctcat gtttgatgaa tattgtgact cgtgattcgg
    30481 agttggcagg agaacaccgt ttatttggga aaaggggtta tgacaaaatt ttgttataat
    30541 cctttttttg tgtcttaaaa aagagagaaa gtggttgaaa ttatatttat cgtatataaa
    30601 acaatccgtg tatagatgct gtattttgtt caccatcatc aaaaaaataa gggaatgtga
    30661 caatactatt gtcacattcc cttattactg tgtattctat taaatcaatc atctttctca
    30721 tctgattctt tggttgcttg attcgctctt gcataagctg aagcggtgtt ccagtaatct
    30781 gtatagtttt catcggtacc gccaacgcca aacttaaagg cgcttttctt cggtccattt
    30841 ttcgcccata gcgattcaac tgtttttcct ggtgtttgga tggatttttt attgacagta
    30901 acttttccag gtgtttgacc agtaaatcca gatacttttt tcttcatcac atcagatgat
    30961 aattcgaatt tctcatcagt gccaaaaatg tcaggttttg cttggtaagc ttgattgatc
    31021 agattggcaa gataattagc cgaacgtatc cttgctttgc tgtccattcc agtattgtca
    31081 tcgtgccctg cccagctact gatggtaata gacggagtag agacaattag ccatgaatct
    31141 cggtattcat tggtagaacc agtttttccg acccaatctg cttttcctag atttccgttt
    31201 aagcttgaga tggcatcttt gaatggtgta gtgatttttg cattgatcac actgcgcatc
    31261 atgtcattca tgatcgatgc cgttgcttcc gaatagatcc ggacagagtt gctttcgtgt
    31321 ttataaatta catttcctgc gttatctgtg atggaatcga tgatatagcc ttcttcatag
    31381 acgccgccgt ttgctaaagc ctgaaatccg ttggtctgtt gaagcgtcgt gacatctact
    31441 gtacctaaag gagcagattc aacgccccaa ttgtcatttg ccgggtagtt catctttgac
    31501 aagtagtgct cgtaagcata ttgtttcgat ccaccggaat tcaacacatc ttgatataag
    31561 tgataagccg ggatattgtt ggaccattct agtgactcac ggacggtttg gaaggtgttc
    31621 gatccttcgt tagtagcatt gacgattttc tcgccagcat tttctccttc ttgccaagtt
    31681 gttgcataat ctgatacacg ggattctgat ccgataagcc ccatgtcgat tgcaggtccg
    31741 taaacaagaa ctggtttgat tgatgagcca gcttgacgtt ctgtatcgaa ggcatggtta
    31801 ttttgattct gactgtaatt ccgtccgcca acgaatccgt agatgcggcc agttctgtta
    31861 tccatcaata catttccggt ttctacttga ctgccagcac catcatctaa catgtatcca
    31921 taattttcaa cgccattttg caatgcagca tagacatctt tatcgattgt cgaatggatt
    31981 gtatatcctt tattttggat cgtttgctgt gccttttgat aataagcgtc actgatcgat
    32041 gaatcagaca tatcagcgtt gtctttttca gccaattgtt gtgcaatgat tcttgttgct
    32101 tcattcatca cagtataata caagaattct ctgtcagtct gttctgcgat ttgttgtggt
    32161 agaaagtctt ttgtaaggtc ataggctttt gcttcttcgt attgttcttt cgtaatttgg
    32221 ccttcacgat acatactgaa taaaacaaaa tcttttctgg caaggccggc agaaagatct
    32281 tcttttagcg cacctgtgtt cgtgtatgga ctatacgtga tcgggctttg gggtaatcca
    32341 gcgatataag cagcttgcgg cagagtaaca tcttttgcat ctacaccaaa gattcctctt
    32401 gcagcttcct ggactccagc aatgttttgt cccttattat tacgaccaaa tggtgagaca
    32461 ttcaaatacg tagcaatgat ctcatctttt gaaaagtatt tttctacttg tgcagaaaga
    32521 atgatctcgt ttgctttacg tttgaatgtt gtttcatctg taaggatctg ctgtttgact
    32581 aattgctgtg tcaaagttga tccaccagaa gagccaatac cagtagcttc agatactaat
    32641 gctcgtaaaa cagctttagg aacgtaacct tggtgtttat cgaagtattc atcctctgtg
    32701 ctgataattg cagttttcaa taaaggtgaa attttatcag atgaaatagt cgtgcgcatc
    32761 agatctgtct ggatttttga tatctctgta ttatctgcat aaacaatctt tgacgtttct
    32821 gtaatgtttc ctagttctgt ctgcagtgct tttttagtcg gcaattgtgt gtcttcgacg
    32881 agataggcaa aatagccagc gcctattcca atacctagag ctgcgaacat caaaagaaga
    32941 acagtaataa aaacagtcaa tgattgaaaa actctaaaaa taatcttagg aacaaaccat
    33001 ttttcttttt ttcttctatt ctgttttttt cgagtatttt tgcgtggcaa aagggtcacc
    33061 tcaactaatg gttttatttc gctaattata gcaaataata gaacaaaaaa acagacaagg
    33121 ctgtttttgg caggaatttc ttttttaaag gagttaaaac gtgagaaaat gatctttttt
    33181 ataaaagagt tcttaagctt ttctttagag aaaatttcag ttactatgga gaacagggca
    33241 aagaaaaatc aatcattttc tttgtatatc atcttaccaa tatataggaa ttgttagtaa
    33301 caattgcctt tcaaggagga atccatccat gttattaggc tcacacgtca gcatgagcgg
    33361 aaagaaaatg ctgttaggag cagcagaaga agcagccagt tatggatctt cgacttttat
    33421 gatctataca ggcgcccctc aaaacactag aagaaaagcc atcgaagaga tgaatattga
    33481 tgccggaaaa gcgtacatgg cagaaaacgg tctaacaaat atcgtcgttc atgcccctta
    33541 tatcattaat ttggggaata cgatcaaacc agaaatgttt ccttttgccg tccaatttct
    33601 gagagaagag attgttcgtg cggaagcttt aggcgctact caaattacac ttcacccagg
    33661 tgctcatgta ggggcaggaa cagcagccgg aattgctaga attattgaag gcttgaatga
    33721 agttttgaca aaagaacaga cggcacaaat cgctttggag acgatggcag gtaaaggtac
    33781 ggaaatcggt cgaacatttg aggaattagc gcaaatcatc gatggagtaa aattgaatga
    33841 aaaattatcc gttacttttg acacctgtca tacgaatgat gccggttata atattaaaga
    33901 agacttttca ggtgtaatgg aagagtttga taagatcatc gggttagatc ggcttaaagt
    33961 actccatatc aatgattcta aaaatccgca aggttcgcat aaagaccggc acgccaatat
    34021 tggttttgga acaatcggat tcgacacgtt gaataagatc gtccatgatc caatgtttga
    34081 caatatttcg aaaatcttgg aaactcccta tgtcggcaaa gataaaaaga aagcttacgc
    34141 accatatgga aaagaaatcg ccatgttcaa agcgcaaaca ttcgatccag aagtgtttcc
    34201 tgagttatta gcagaagaag tgatactcta agattttcta tttgacagca gcaaaaaagg
    34261 accaggcaca ttgccttgtc cttttttgtt taagaaacaa aggagtactt tttgcagtct
    34321 ttttcatcat gcatcttctc ccccctcttt ttaaatttag acaaacaggg tatactggta
    34381 gaaaagtaaa aaaacgaaaa gtttcacaag caagtggcaa aacgtgattg gaaggacgaa
    34441 aagatgctaa acactttaaa acccttgatc aaccaactga ctaagaaaca tacatacttt
    34501 attcttggca tagccctatt atttacaatt attcgtttgt atttatatag taaagcaatc
    34561 tatggaacag acatgtcggc aacctatgat gatcagttgc tgattcgcta tgctcaaaat
    34621 attttggacg gtaaatggct aggggagtat acgactacaa cactttccaa aggaatctcc
    34681 tacagcttat ttttagtact tgcacataaa ctgcattttt cttatgcagt tttattcggc
    34741 atgttcagta tttttgccag tatattggtt gcactcgcgt ttaaaccgat cgtgaagaac
    34801 aagtatcttc ttttaagtat ctatttttat tttttgtttt ctccgattag ttttacacat
    34861 gaatattcca ctcgaacgta tcgtaatgcc ctcgtgattc cggctgttga gatcattatc
    34921 gcttgtttat tagctattta ttaccgcaaa tacggttcta gcagaaaatt gctgccatgg
    34981 tttttagggc tttctgtcgc ttttcctttt ttttggttca ttcgcgaaga ttctatctgg
    35041 cttcttcctt ttactgtagt agctttgggt atcagtttcg tccagatctt gctagggcaa
    35101 gacttttctt tcaaagaagt aaagaagtta aaaaaacgac tctttcttct ctcgaaacgg
    35161 gaatggctga aactttcatt gtttttactt cctatagttg gcttttccat cgtctatcag
    35221 tggatcgaat ataaaaatga gacaacatac ggtattgcaa ccatcaatga ccgttcgagt
    35281 ggagaatttg gacgtttgat gaaacaactg atccgaatag atgatgaaac gaacttgaat
    35341 aaagaaaaca gtgagatatg ggtctcacat gctgcactaa ataaagcatt agccgcatct
    35401 cctacattcc atactgtttc tacccgtatt gacgaattat atacgactca cgcgtggact
    35461 caaggtggaa aagtaaaaga attacccgga gatatcatct tttgggcatt gcgagatgta
    35521 gtaagcgaat ctggttatta ccaaaacaat gcaaaagaaa ccaatgattt ttggaaaaaa
    35581 gtcaaccaag aattagaaga agcctatcac aagggaacca tcaaaaagaa aaaagagttc
    35641 tatctgatgg gatcaggaga tggaaagacg atgagtgatc tgccaatcgt attcgatttc
    35701 cttcaatcag gatacgctta tgcatggcga tataaagatt tccatcaagg aggaaattat
    35761 agtttcggaa cgcaagaaca agtcaaacag gctcaagaat tattaaacct tccgctaatg
    35821 gagaattgga tcgatagtaa tgaaccgaga acacctaact tccaattaac aaaatcagca
    35881 aaagtcgcga atgtcatcat aaaaatttac caagttctgg ctattcccgt gctgattgct
    35941 tcaatagcag ggatattact tatgatacta ggaaccgtat ttgcaaaagc tgatcgtgcc
    36001 tattatagtt cctctttgat tgtggtcacc ggattaatac tgacacaatt ggtttttctt
    36061 ttcggtgtct catggttttg ttcttatgcg ccagaaaaaa aggactattt tttaagtgtc
    36121 tacactggtg caggcgtgcc attaacgcaa ttcatcgtag taacaagcat gctagttttc
    36181 agtaaattaa atctgaaaaa agctttatct tctaaataaa aagacccttg gtagctagat
    36241 ttttaaccta actatcaagg gtctttgtgt ggaaaaatca gtttttattc gatttcttct
    36301 accgtttcct gcaaaagctg ttcatatttt ttgtttcctt tatataaatc accaatcgtc
    36361 catgccaaca ggagaaggac aagaacaatg attacataag cgatgtgatc agccaatgcc
    36421 agttgagcgg aagtggcttg ctcgccgaag aaactttcga tctgtcctgg taatcctcgt
    36481 aagttcaagt aagttagact aatcacagag aaccagccaa gaagttttac gatgaatgaa
    36541 tttttaaatc ggctacccat ctccgcttgg ctgttggtca tcatcagtaa aggaatcatc
    36601 gaaaatggca acgcaaaagc caagaacacc tgggattcat tcattaagtt attcaaagcg
    36661 gtatgttctt cgatcgttcc ttttgtactt gtaaacaaaa cacaaatcaa tacaggaatc
    36721 acagaaagca atcgtgtgac cagtcttctt agccaaatcg ggatacgcat atggatgaat
    36781 ccttccatga tgacttgacc ggtcaacgta ccagtaatag tagagttctg gccagaagca
    36841 agaagtgcta cagcaaataa agtggacaag ataccagatt ttgctacagc aatcaaaata
    36901 ccattgctca tagctgatgg atcagataaa gcttcataca gaccaaaaaa ggaaggatct
    36961 tggacagaac cggttttgaa gacagcgaca cccatgatca atagcagtgc attgactaga
    37021 aaagccattg acaactggat attagaatcc caagtagaaa aacgtacagc ttgtgctaca
    37081 tcttcttcgt tttggtgatc gatcttccga gtttgtgaaa ctgctgaatg aagatataag
    37141 ttatgcggca tgactgttgc accaataatc cctaaagctc cagttagcgg agtttcgcca
    37201 ccaatgacag gtgagtctga gaatgtatgg ctgttaggga taaagccagc aaataaactg
    37261 tgccaatctg ggttagacaa gccaacttga tagacaaaaa cgaataaaat gacgaaaatt
    37321 aagcagacaa ccagtgcttc gatcttacga aacccgactt ttgtcagtag taagagaacg
    37381 aaaacatcta atacagtaat aaagacggca atcaccaatg gaatatcaaa aagtaagtag
    37441 agggcgattg ctgcaccaat tacttctgca atatctgttg ccataatggc taattcagtc
    37501 aagatccata aaatgacacc taatgtctta cttgtgcgtg ctcggattgc ctgtgccaaa
    37561 tccatttggc tcacgattcc taattttgct gccatatact gtaaaagcat agcaataaga
    37621 ctagacatca aaataactga catcaataaa tattggaagt tttgtcctcc ggtgatcgaa
    37681 gtagaccagt tacccggatc catataccca actgctacta acgcaccagg tccagagtaa
    37741 gcaagaagtg ttttaaaaaa acttttcccc tttggtacat caactgttcc atttacttca
    37801 gctaaagata aaccatttgt atgttcaata agtcgatgct tatgaaattg tttattggat
    37861 tgattattca agaaaaaacc acctttccta tttatctacg attacattct acacctcaaa
    37921 aagaaaaaat taaagaaaaa gattcggtat gcctaattta ttttcgtggc gtgtcttgct
    37981 agatgacgaa agaaattttg ttctcctcta aaaaaaccgc tattttttgc atgaaactaa
    38041 agaatacgcc tttctttttt caataaactt tagtaaaata agtcattgag agcaattcgg
    38101 aaatagaaag gagagattta tatgttatca acaggtattg tcgtacttat tgctattatt
    38161 gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc gtaaatatat gaaagactat
    38221 ttagaaaaaa atcctcccgt taatgaggac atgttgcgat caatgatgat gtcaatgggg
    38281 caaaaaccat ctgagaagaa aattcgccag atgatgcagc aaatgaagaa ccaagggaaa
    38341 aaataggttc tgacccaacg actttttacg ggctgatttt ttcagcccgt tttttatttc
    38401 gtaatttgtt agactattca gattattcag caaaaaagac aagcacagac aatcctaggc
    38461 tcttgataaa gacaaaggag gagtaaaatg tcgattttta gaaaattagg ctggtttttt
    38521 cgtcaggaaa agaaacatta tttaattgga gttttctcgc tattcgcagt ggccttggta
    38581 cagttagttc ctccaaaagt gattgggatc atcattgatg agattgccga taaaaattta
    38641 tcaataaaga tcattttcgg gtggatcttc ctattgatta tcgctgcttt tttacaatat
    38701 ctttttcgtt atatctggcg gacgaatatt tggggaagtg cagctcggtt ggagaaagaa
    38761 cttcggcagc agttattcaa acattttaca gaaatggaca atctctttta ccaaaaatat
    38821 cggactgggg atttgatggc tcatgcaacg aatgatttga atgcgatcca gaatgtagca
    38881 ggagctggta tcttgacctt tgcagattca gtgattacgg gcggaacaac gatcattgcg
    38941 atgattttgt ttattgattg gcgcttaaca ctgattgcga tgattcctct gcctttgctg
    39001 gccatcacct cacgagtgct gggatcgaaa ctccatgatg cttttcgtga ttcgcaagaa
    39061 gctttttcta gtatcaatga taagacacaa gaaagtatca ctgggatcaa agtcattaaa
    39121 acatttggac aagaaaaaga agatctagcg gattttgaag aaaagattga agatgcaatt
    39181 attaaaaaca aacgagtcaa ttttttagac tccttatttg atccattcat taccttgatt
    39241 atcggtctgt cttatgttgc aacaattatt ttaggtggaa agtatgtctt ggaaggcacg
    39301 atctcgatcg ggcagatggt ctcatttatt agttatatcg gtatgcttgt ttggccgatg
    39361 tttgcgattg gtcgattgtt caacgtattg gaaagaggaa atgcttctta tgatcgcgtc
    39421 aatgaacttt tgcatgaaaa aacgcatatc gtggaacaac tgggtgcagt gcagaccccg
    39481 gcaaaaggcg agctgaatgt agctatttcc tcttttaatt atctgaaaga agaatcacca
    39541 gcattagaac aaatcaagtt tgcaatccaa gaaggagaaa cattaggtat tgttgggaaa
    39601 actggtgcgg gaaaaacgac gatcatcaaa ttactgatgc gtgaatacga tgattatcaa
    39661 gggaaaatcc aatttggcgg acgttcgatc aaagaatatt cattgaatgc tttattagga
    39721 tcaatcggct atgtgcctca ggatcacttc ttgttttcga tgaccattcg agataatatc
    39781 cgtttttcta atccaaaact ttcagaagaa gaagtaaagc atgcggcttc tttagcattt
    39841 atcaacaatg aaatcaagga attaccaaaa ggttatgata ccttagttgg agaacgtgga
    39901 gtctctttat ctggcggaca gaaacaaagg atttcgattg ctcgagcatt gatcaaaaat
    39961 cctgaattat taatgttaga tgatgcactt tctgcagtag atgccaaaac agaagaagct
    40021 atcttgcata atttaaaaga aataagaaaa gagaaaacaa ctatcattgc agctcacc
//
LOCUS       DACEDM010000019        38235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEDM010000019 DACEDM010000000
VERSION     DACEDM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.019"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IWO50_000904"
     CDS             complement(451..1761)
                     /locus_tag="IWO50_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0358042.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IWO50_000905"
     CDS             complement(1773..3191)
                     /locus_tag="IWO50_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0358043.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IWO50_000906"
     CDS             complement(3431..4999)
                     /locus_tag="IWO50_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0358044.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IWO50_000907"
     CDS             complement(5003..5413)
                     /locus_tag="IWO50_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0358045.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IWO50_000908"
     CDS             complement(5410..6333)
                     /locus_tag="IWO50_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0358046.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IWO50_000909"
     CDS             complement(6330..7208)
                     /locus_tag="IWO50_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0358047.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IWO50_000910"
     CDS             complement(7195..8532)
                     /locus_tag="IWO50_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0358048.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IWO50_000911"
     CDS             complement(8535..9638)
                     /locus_tag="IWO50_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0358049.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IWO50_000912"
     CDS             complement(9712..11220)
                     /locus_tag="IWO50_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0358050.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IWO50_000913"
     CDS             complement(11186..11878)
                     /locus_tag="IWO50_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0358051.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IWO50_000914"
     CDS             complement(11900..12874)
                     /locus_tag="IWO50_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0358052.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IWO50_000915"
     CDS             13174..14046
                     /locus_tag="IWO50_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0358053.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IWO50_000916"
     CDS             complement(14504..16702)
                     /locus_tag="IWO50_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0358054.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IWO50_000917"
     CDS             16934..17926
                     /locus_tag="IWO50_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0358055.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IWO50_000918"
     CDS             17986..19407
                     /locus_tag="IWO50_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0358056.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="IWO50_000919"
     CDS             19400..20899
                     /locus_tag="IWO50_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0358057.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="IWO50_000920"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="IWO50_000920"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0358058.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="IWO50_000921"
     CDS             22491..23174
                     /locus_tag="IWO50_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0358059.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="IWO50_000922"
     CDS             23176..24012
                     /locus_tag="IWO50_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0358060.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="IWO50_000923"
     CDS             24023..25777
                     /locus_tag="IWO50_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358061.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="IWO50_000924"
     CDS             complement(26053..26394)
                     /locus_tag="IWO50_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0358062.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="IWO50_000925"
     CDS             complement(26462..28633)
                     /locus_tag="IWO50_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0358063.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="IWO50_000926"
     CDS             28844..29701
                     /locus_tag="IWO50_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0358064.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="IWO50_000927"
     CDS             complement(29772..30629)
                     /locus_tag="IWO50_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0358065.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="IWO50_000928"
     CDS             complement(30614..33316)
                     /locus_tag="IWO50_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0358066.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="IWO50_000929"
     CDS             complement(33329..34618)
                     /locus_tag="IWO50_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0358067.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="IWO50_000930"
     CDS             34768..35814
                     /locus_tag="IWO50_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0358068.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="IWO50_000931"
     CDS             35816..37969
                     /locus_tag="IWO50_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0358069.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11281 a         7861 c         6802 g        12291 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctg
//
LOCUS       DACEDM010000020        37352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEDM010000020 DACEDM010000000
VERSION     DACEDM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.020"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..323)
                     /locus_tag="IWO50_000932"
     CDS             complement(<1..323)
                     /locus_tag="IWO50_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HAQ0358070.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLH"
     gene            complement(320..1732)
                     /locus_tag="IWO50_000933"
     CDS             complement(320..1732)
                     /locus_tag="IWO50_000933"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0358071.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(1729..2265)
                     /gene="cysE"
                     /locus_tag="IWO50_000934"
     CDS             complement(1729..2265)
                     /gene="cysE"
                     /locus_tag="IWO50_000934"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0358072.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(2587..4047)
                     /locus_tag="IWO50_000935"
     CDS             complement(2587..4047)
                     /locus_tag="IWO50_000935"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0358073.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(4217..5374)
                     /locus_tag="IWO50_000936"
     CDS             complement(4217..5374)
                     /locus_tag="IWO50_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0358074.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(5408..6778)
                     /gene="radA"
                     /locus_tag="IWO50_000937"
     CDS             complement(5408..6778)
                     /gene="radA"
                     /locus_tag="IWO50_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0358075.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(6841..7368)
                     /locus_tag="IWO50_000938"
     CDS             complement(6841..7368)
                     /locus_tag="IWO50_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0358076.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            7598..8404
                     /gene="proB"
                     /locus_tag="IWO50_000939"
     CDS             7598..8404
                     /gene="proB"
                     /locus_tag="IWO50_000939"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0358077.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            8401..9648
                     /locus_tag="IWO50_000940"
     CDS             8401..9648
                     /locus_tag="IWO50_000940"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0358078.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            9724..10068
                     /locus_tag="IWO50_000941"
     CDS             9724..10068
                     /locus_tag="IWO50_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358079.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(10185..10511)
                     /locus_tag="IWO50_000942"
     CDS             complement(10185..10511)
                     /locus_tag="IWO50_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358080.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(11126..12328)
                     /locus_tag="IWO50_000943"
     CDS             complement(11126..12328)
                     /locus_tag="IWO50_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0358081.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            12445..12960
                     /locus_tag="IWO50_000944"
     CDS             12445..12960
                     /locus_tag="IWO50_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0358082.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(13159..13605)
                     /locus_tag="IWO50_000945"
     CDS             complement(13159..13605)
                     /locus_tag="IWO50_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0358083.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(13652..15130)
                     /gene="msr"
                     /locus_tag="IWO50_000946"
     CDS             complement(13652..15130)
                     /gene="msr"
                     /locus_tag="IWO50_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0358084.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(15484..16113)
                     /locus_tag="IWO50_000947"
     CDS             complement(15484..16113)
                     /locus_tag="IWO50_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0358085.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(16097..16561)
                     /locus_tag="IWO50_000948"
     CDS             complement(16097..16561)
                     /locus_tag="IWO50_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0358086.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(17048..18316)
                     /locus_tag="IWO50_000949"
     CDS             complement(17048..18316)
                     /locus_tag="IWO50_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0358087.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDCLTPMGWRYEGIGFYGY"
     gene            complement(18547..19284)
                     /locus_tag="IWO50_000950"
     CDS             complement(18547..19284)
                     /locus_tag="IWO50_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0358088.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(19351..19920)
                     /locus_tag="IWO50_000951"
     CDS             complement(19351..19920)
                     /locus_tag="IWO50_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0358089.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            20048..20920
                     /locus_tag="IWO50_000952"
     CDS             20048..20920
                     /locus_tag="IWO50_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0358090.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            21262..22134
                     /locus_tag="IWO50_000953"
     CDS             21262..22134
                     /locus_tag="IWO50_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0358091.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            22131..24089
                     /locus_tag="IWO50_000954"
     CDS             22131..24089
                     /locus_tag="IWO50_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0358092.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(24421..25272)
                     /locus_tag="IWO50_000955"
     CDS             complement(24421..25272)
                     /locus_tag="IWO50_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0358093.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            25395..25862
                     /locus_tag="IWO50_000956"
     CDS             25395..25862
                     /locus_tag="IWO50_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0358094.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(26125..26607)
                     /locus_tag="IWO50_000957"
     CDS             complement(26125..26607)
                     /locus_tag="IWO50_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0358095.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(26707..27570)
                     /locus_tag="IWO50_000958"
     CDS             complement(26707..27570)
                     /locus_tag="IWO50_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0358096.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            28168..29043
                     /locus_tag="IWO50_000959"
     CDS             28168..29043
                     /locus_tag="IWO50_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358097.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(29188..29340)
                     /locus_tag="IWO50_000960"
                     /pseudo
     CDS             complement(29188..29340)
                     /locus_tag="IWO50_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(29608..30939)
                     /locus_tag="IWO50_000961"
     CDS             complement(29608..30939)
                     /locus_tag="IWO50_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0358098.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(31088..31801)
                     /locus_tag="IWO50_000962"
     CDS             complement(31088..31801)
                     /locus_tag="IWO50_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0358099.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            31976..33400
                     /locus_tag="IWO50_000963"
     CDS             31976..33400
                     /locus_tag="IWO50_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0358100.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            33699..34406
                     /locus_tag="IWO50_000964"
     CDS             33699..34406
                     /locus_tag="IWO50_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0358101.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            34393..34716
                     /locus_tag="IWO50_000965"
     CDS             34393..34716
                     /locus_tag="IWO50_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0358102.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(34805..35842)
                     /locus_tag="IWO50_000966"
     CDS             complement(34805..35842)
                     /locus_tag="IWO50_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0358103.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(36163..37146)
                     /locus_tag="IWO50_000967"
     CDS             complement(36163..37146)
                     /locus_tag="IWO50_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0358104.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT        10989 a         7712 c         6694 g        11957 t
ORIGIN      
        1 aaatgcagat agcccatgag cgcttcgaac cctgttgcga cccgatacgt cgtaacatca
       61 gcattttttg cgctcgtatg gcttttagca tttcttcctc tgcgataata aagttcttct
      121 tcttctgtca aaagatgttc ttccagcatt tgctgaatca aaaaggcttg tgcattcgca
      181 gaaacataat tcgttgccgc acgatgcagc ttattgggtt ttgtttctcc ttgatcaatc
      241 aggtggtcac gaacatagat ttcgtaaatc gcatctccaa cataagccaa ggctaaaccg
      301 ttcaattgtg tgtaatctct catgcttctc ttctccatcg ggttccttgc ggtgtatctt
      361 ctaataagat ccctttttct ttcaacaaat cacgaatttc gtcgcttcgg acaaaatcac
      421 gattgcttct tgcttgattt cgttcttcga tcaattgttc aatctcttca tctaataact
      481 cttctgctgt aaataaaata ccgaagatcg aaagccattg cgagaataaa gcttctcctt
      541 gtgccaagac agcggcagaa actttcggct ggtctagata agtgttcagc cacttagcaa
      601 gctcatagac cactgtgatc ccatttgctg cattaaaatc atcgtccatt tcttcgatga
      661 atcgctgttc taaattttct aacaaagcga ttttatccgc atcatctgcc aaattctctg
      721 aggcagtttc ttggcggaat ttcaaattgt caaacgtatt cttcagtcgt tgtaaattgg
      781 tggctgcttc tttcatcgtc gtttcactgt agcggatcgg acgacggtat tgtgtggtcg
      841 ccatgaagaa acgcaagact tgtggatcaa cgtttttgat catttcatgt acagtgatga
      901 aattccctag agatttactc attttttcat cgtcttcgcc aatcgttaca tagccattgt
      961 gcatccagta attagcgaat tttttccctg ttttcgcttc actttgtgcg atttcatttt
     1021 catgatgagg aaattctaaa tcttgcccgc caccatggat atcgatcgtt tctcctaaat
     1081 gttttgttgc cataacggaa cattcaatat gccaaccggg acgtcctttg ccccatggtg
     1141 aatcccaaga aatctcgcca ggttttgctt gtttccataa agcgaaatct aatgggtctt
     1201 ctttttgctg ctgttcttcc cctgtacgct gacttgctcc gacttccagt tcatcgatgg
     1261 attggtcact taatttccca taattttcaa atttacgggt acggtaatac acatcacctg
     1321 aagattcata agcatagcct ttttcaacca acacttgaat aaatgctaaa atatctggca
     1381 tatgatccat gactcttgga tgcagtgtag caggttcgac attcaacgct ttcgtatctt
     1441 cttcaaatgc tcggatgaaa cgatcagcaa cttctggtgc tgtaatacct aattcgtttg
     1501 ctgtacggat gattttatcg tctacatccg tgaaattcga tacgaaatcg actttatagc
     1561 ctcgatattc aaaatagcgt cgaattgtat cgaaagcaat ggtgctacga gcatttccga
     1621 tatggatata attatacaca gtcggtccgc agacatacat accgacatgt cccttttgta
     1681 tagggatgaa ttcttctttt tttctggtca atgtattgta gatttttatc attctgatct
     1741 cctcactttt tctcctttga ttcgaacgac ttttgctggt actcctaccg ctgtagcgtc
     1801 gtctggaaca tctttcaaca caacggcacc tgcaccgatt ttggcgtttt ttccgatcgt
     1861 taccggtcct aatatctgcg cattcgcaga gagcatggct ccttgtttaa cagttgggtg
     1921 tcttttaccc gtttcttttc ctgtgccgcc taatgttacg ccatggaata aaatcacatc
     1981 gtcctcgatt tctgcggttt cgccaatgac gattcccatt ccatggtcga tgaacacgcc
     2041 agttccaatc gttgctcctg gatggatctc aacaccagtt aggaaacgcc agaattgagc
     2101 attgatcttc gccaataaaa agagccggtg ctgatataaa aaatgagaga aacgatgcca
     2161 aaacaaggca tgaaaaccag ggtatgtcaa aacgatctca gctgtcgaac gtgcagccgg
     2221 atcgtttctc tttgctgcat caatcgctcg ggtaaaccaa ctcatgactt tccactcctt
     2281 tcgtaagaat tttatgtagg aaatcatctt cttataaaga aaagaagcgc cttttaaata
     2341 agtttcctta tcaaaagacg ctttccgcgt ggttccactt ttcttcataa tcaatgatta
     2401 tctctgacag ctaacgcctg ttatacgttc ttggctactt tgtttcctat aaaacagttt
     2461 ttcctgtttt ctgtgtttcg ccaagaccac tcccagaggc atttcggcaa ccattcattg
     2521 ataccttgca ccacccggta tctctcttgt aatgatcagc tgcttacttt tttctgttca
     2581 tcgtgtttag ttcagtactt gatttaaatg tgctaatgat ttttcacgac caagcaattc
     2641 gatcgtttca ggcaattctg gaccatgcat ttgtcctgat acagctacac ggattggcat
     2701 gaaaagattt tttcctttta cgccagtttc tttttgaaca gctttgatgg cagctttgac
     2761 agttggagca tctactactt ccatttgttc tagctgtgct ttgaatgcgt ggagaacagt
     2821 aggaactgtt tcaccagcaa gtacttcttt tgctgcttca ttcaatacag gatgttcatt
     2881 gaaaaatagc gtagacagat caacgatttc tgcagcatag ctcatttgag gttgatacag
     2941 gctaacgatc ttcttcaccc attcgattgt cttttcatct gggttttctg ctacacgtcc
     3001 atcagcgatt agataaggca gacacatgtc agtaaatgca tctagatcca ttgctttgat
     3061 gtaatggttg tttacccatt gcagtttttt cgcatcgaaa gctgcgggtg atttacttag
     3121 gcgttttgga tcgaacattt tgatcaattc ttggtgttcg aagatttcgt cttcgcccac
     3181 aggtgaccag cccaataatg caataaagtt gaacattgca tctggtagat agcctaattc
     3241 gcgatattgt tcgataaatt gcaagattga ttcgtcacgt ttgcttagtt ttttacctgt
     3301 ttcactgttg atgatcaatg tcatatgtcc gaaggttggt ggtgtccagc caaatgcttc
     3361 atagatcatc agttgttttg gtgtattggc aatatggtca tctccacgta aaacgtgagt
     3421 gattttcatc aaatgatcat cgaccgcaac agcaaaatta taagttggca taccatcgcg
     3481 tttttggatg acaaagtcgc caccgatatt atcggattcg aacgaaatag cgcctttcac
     3541 catatctgta aatgcgtatt ctgtattgcg aggcacacgg aaacgtacaa cagattctag
     3601 accttgtgct tctttttctg cttgttcttc aggtgtcaag ttcgcacatg ttcctgcgta
     3661 atgaggcatt tctcctcttg cgcgttgtgc ttcccgttct gcttctaatt cttcttctgt
     3721 gcaatagcat ttgtatgctc gattgctagc aagcagctga tcaatcagcg gttgatagat
     3781 ttctttacgt tcagattgac ggtaagggcc gtattctcca ggattttcag gagattcatc
     3841 ccaattcatt cccaaccaag caaggttttc caactggctt ttttcgccat cttcgatatt
     3901 acgtttttgg tcagtatcct caatacgaat aataaagtct ccatcattgt ggcgggcaaa
     3961 caaataattg aataatgcag ttcttgcatt tccaatatgc aggtgcccag ttggacttgg
     4021 tgcatagcgt acgcgtactt tcgtcatttt cttcctcttc tttcaaacta gactttgact
     4081 aacagcaaaa attccgtcag ttacggcttc tcaaatatca tttatttaaa gccactcagc
     4141 acaaatttta ccttttctaa ggtaaatagt aaagccaact ggttgaattt ccttaccagt
     4201 tggctgaatt tcttttttat tttgattgtt tagtcttttt ctctgtctct tttttattgt
     4261 cgattcctcg tcctgcatgt gcaggtttag caaagatcat gcgtccagca gctgtttgaa
     4321 gggcacttgt tacaacgact tcgatacgtt cattcatgta atgctgtcca tcttctacga
     4381 caaccatcgt gccgtcatca aggtaggcta ctccttgttg gcgttctgtc ccggctttta
     4441 ctactagaac attcatcgtt tcaccaggga tgacgactgg ttttacggca tttgccaacg
     4501 cattgatatt aagaacaggt acattttgga attcagagac tttattcaag ttatagtcat
     4561 tggttacaat gacaccatct aaaagcttag ccaatttgat caatttgctg tctacttcac
     4621 tgatatcttc aaagtctcca tcgtacattt cgacagatat accctcttct ttttgcaagg
     4681 catttaggat atctaatccg cgacgaccac gcacacgttt caaactatcc cctgagtccg
     4741 cgatgtattg tagttcgtaa agaacaaaat tagggatcat caaggttcct tctaagaatc
     4801 cagtctttgt aatatcataa atccgaccgt caatgatcac gcttgtatcc aagattttat
     4861 atttatggaa attttctcct gattttcgtt ctaacagttg actctcaact tgactagctg
     4921 tttcttgctc gatttttttg cttcgtgaac caaaaacttt cttccattct tcgattcgtg
     4981 tcgttcccat tctcaatccc agatagccta agatgatcat cagtaaaatc ggcaatacac
     5041 tattgacgaa tggaatttcc atattgtaaa gaggggtcga gatcacaaca cctaaagtca
     5101 atccgatgat cgcaccgatc gcaccgaaga gaagataagt caagctgacc tcacttagtt
     5161 tttgttctac ttgtttgata atagctgaga tatgtttggc caacagcaaa gataataaaa
     5221 agaaaataag tgcgcctaac aggctgttgg taaatgtatt attcagccat ccgtttgact
     5281 gttgaccgat tgcctgccaa gctgagggta aaaatgaaat acccaagctt gcgccgacaa
     5341 tgatcatcag caaagtaatg acacgttttt gcatcgtttt tcctccgttt cgacaatcat
     5401 ttattagtta gcggaacaca cgcttcaacg tttcagccaa agttgctact ccaactatct
     5461 cgattccttt tggcgcttcc cagttaccta agttgttttt aggtaggtat acttttgtga
     5521 agccgagttt ttggacttct ttgactcgtt gttcaatatg actgacacgt ctgatttctc
     5581 cagtcagtcc aatttcaccg atgaaacatt ccgttggctg tgttcctttt tctttatagc
     5641 ttgaagcaat actgacagcg atagctaaat caatcgctgg ttcatttaat tttaccccac
     5701 ctgcagcttt taagtaagca tcttgattct gaagaagcaa cccagctctt ttttccagca
     5761 ctgccatgat cagagatacc cgattgaaat caagacctgt tgttgttcgt ttggcatttc
     5821 cgaacatagt aggtgtgact aatgcttgga tctctaccaa aattggacgt gtcccttcca
     5881 ttgccacaac gatagcagag ccagttgcac cgtctaggcg ttcttctaag aaaatctgtg
     5941 aaggattagc gacttcttcc aatccatgct catgcatttc aaaaatccca atctcattcg
     6001 ttgagccaaa tcggtttttt actgctcgca aaatccgaaa actatgatgt tgttcacctt
     6061 caaaatataa caccgtatcc accatatgtt ctaacattct tgggccggcg atcgagcctt
     6121 ctttggtaac atgaccgacg ataaaaatgg ctatcccatt tgttttagcg gttttgagta
     6181 gttctgcagt ggtttctctt acttggctga cacttcctgc cacacttgtg atgtctggct
     6241 gcgtcatcgt ttggatggaa tcaatgataa cataatcagg ggagatatgt tcgatggcac
     6301 gactgatttc gttcatatct gtttcagcgt atagataaaa ttctgtgttg atacttccga
     6361 ggcgttccgc gcgtaatttt atctgctctg cgctttcttc gccggataca tataagactt
     6421 ttccgcctat ggctgctaat tgctgagaaa cttgtaacag caaagtggat ttcccgatac
     6481 ctggatcgcc accaatcaag accatagacc ctggcaccac acctccgcct aacacacggt
     6541 tcagttcttc taattttgtt ttgatccgag gttctttttt agggacgacg tctgcgattt
     6601 ttgttggttt tgctttttct cctgtcaaac ttgtccgagt tctgcggtcc gttgtatctt
     6661 gttcgatttc ttcgaccatc gtattccatt ttccgcaatt tgggcagcgt cccaagaact
     6721 taggcgacac atacccgcaa ttttggcaaa caaattgtac ttttgatttt tttgccattg
     6781 taccgcctcc ttttatccta atcatgagac attaaatcaa gcttaagtat ctttaacgtt
     6841 ttatttactg gaagaaccaa atccgccttg acgttgagca gtcgtttgat cttgatctgc
     6901 ttttaaaaac ggcaaaaaga tcccttggcc gatacgctcg ccttttttga tcactacatc
     6961 cttgaaacca aagttggtga attggaacat gatatggcct tcatttgcct cattattgta
     7021 ataatcactg tcaatcacac ctactccgtt tgtcaacact aaaaaccgct tcaacggatt
     7081 gcttgaacgg ttagcaagct gcaaaaattc atcttctccc atgtaagctt tgattccagt
     7141 aggcacgagg gttggtttca attgtttttc attttctgat acatcttttt tcaatccatc
     7201 tgcgactgtt tccaacccct ttttccagcc tgctttccag atacttggaa tcacgacctc
     7261 ttctgctgct tcaaaatcat agccagcagc atgatgtgta gcgcgctgcg gtagattgat
     7321 attttgatct ttatatttag aaataacttc aaatcctctt tttctcaccg gttttcctcc
     7381 ctaaataaac tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt
     7441 cttcaagaaa ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg
     7501 caagttgtac aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga
     7561 acgaaattaa tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc
     7621 aaaacgaatt attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa
     7681 tcttgcggca atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga
     7741 agtgatctta gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga
     7801 acgtccgact ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat
     7861 gaatatctat aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac
     7921 aagagacgtg gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct
     7981 tttgattatg gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt
     8041 agatcatctg acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt
     8101 cgcggcagac cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac
     8161 tacaaatccc gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc
     8221 acaagcaggt ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc
     8281 cgcttctagg atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc
     8341 aattattttt gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa
     8401 atgactgact tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt
     8461 atgcctacaa accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat
     8521 caacaagcga tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc
     8581 agtgaaacga tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca
     8641 gagatcgaaa aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg
     8701 aacaccgacg gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt
     8761 tacgaatctc gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat
     8821 gccgttatcc ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta
     8881 ttgcagcaag tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat
     8941 acttctcatg aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt
     9001 cctcgaggcg gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt
     9061 gaaacaggta caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca
     9121 gtgaacatca tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca
     9181 ttgctgatcc attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta
     9241 gaacacgggg tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct
     9301 ttagcagaag acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg
     9361 gtcgattctt tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa
     9421 acgatcgtga cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg
     9481 gctgtctatg tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca
     9541 gagattggta tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta
     9601 acttccacaa aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga
     9661 aacattattc ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc
     9721 aatatgtttg aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct
     9781 tttctgggag tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt
     9841 caacgggaaa aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc
     9901 ttgcacatta gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt
     9961 ttcgatcatt caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct
    10021 tataaatatc agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta
    10081 cgataagtag agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac
    10141 aaagattttg tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt
    10201 aaatctgttt cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa
    10261 cgaagcagct catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca
    10321 gccagtagct gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt
    10381 ctagtttcac cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac
    10441 cgatcatctg aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat
    10501 tcttccagca tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg
    10561 aaaattttcc agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca
    10621 tccccttttt ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat
    10681 tcaacaaaca tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca
    10741 gtctcacaac ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa
    10801 acggtaaaat atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc
    10861 tgattttaca acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa
    10921 aacggcaaaa tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc
    10981 tgattttaca acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca
    11041 accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga
    11101 cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt
    11161 gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc
    11221 tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa
    11281 agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag
    11341 caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga
    11401 tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata
    11461 agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa
    11521 cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta
    11581 attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat
    11641 tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac
    11701 cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg
    11761 ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac
    11821 tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat
    11881 acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag
    11941 tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga
    12001 tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca
    12061 atgttttttt caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag
    12121 tgactaacat gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg
    12181 cagcaatcgt ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg
    12241 tagctgctac aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta
    12301 tatctatttg attctccaat atttccatcc ctttctctct attagcatca tacatgattt
    12361 attttacatg tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa
    12421 aataagacga gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt
    12481 ttatccaaac cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc
    12541 catcacagtc agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt
    12601 ttggacaaag acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct
    12661 cttgtagagg aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta
    12721 gcaaaaatct atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact
    12781 agagaacaac agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg
    12841 ctagacaaga aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt
    12901 cagcaggaac tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa
    12961 gggataaaca gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga
    13021 gcaatacagt tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat
    13081 cactattttt ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt
    13141 tcaaataaca gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag
    13201 cgggccaacg atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt
    13261 catataagca gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga
    13321 catacatgtc accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt
    13381 gctttctgga tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa
    13441 gacatccaaa atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa
    13501 atgaagatct tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca
    13561 catcattgtt gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt
    13621 taaatttgtc acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta
    13681 ttttctgccc ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat
    13741 gagaagtaaa caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg
    13801 ttgccatatc aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa
    13861 aaagcaaagc aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg
    13921 tcaaagtttc ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa
    13981 actctgtttg tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct
    14041 tttgctgata tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc
    14101 ggatatgttc taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt
    14161 tacctaaaga aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt
    14221 ggacattttg tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa
    14281 aacggatttg tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct
    14341 ctaatgtttt cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag
    14401 acaaccgatc cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt
    14461 ccgcttgttt tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg
    14521 actctgcttc tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat
    14581 aattccccga aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct
    14641 catctagaaa atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct
    14701 cttcaatcag tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca
    14761 tcccttgttc ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc
    14821 tccatttccg ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat
    14881 ttaggtcctt tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg
    14941 tttggatttt cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt
    15001 taccgacacc attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat
    15061 caatagatag ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta
    15121 aattttccat atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa
    15181 agagcagaaa aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa
    15241 taactatgac taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa
    15301 aaatggacat actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt
    15361 tgttattcaa aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt
    15421 ttattaggtt accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac
    15481 cttttattcg aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc
    15541 taccgctttt cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa
    15601 tgcctctgcc aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc
    15661 acgcgcaact aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt
    15721 catttcttct cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct
    15781 gacttctttt ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc
    15841 gtaacattgc atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt
    15901 attgaaacgg atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa
    15961 gcgccatggt ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag
    16021 catttcttcg tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt
    16081 cataattgtt gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg
    16141 ttatattgca aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta
    16201 catcgctgaa agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct
    16261 tgattttctt ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat
    16321 ttctctgacg cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc
    16381 cttgatcaat ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca
    16441 gtctcatcat gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga
    16501 cgattttttg atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca
    16561 taatttcctc ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa
    16621 aaaagaaaaa agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta
    16681 tagagaaaag aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta
    16741 aaaccaagat tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt
    16801 ttacaccgcc aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat
    16861 tggatcagta tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt
    16921 ttgagtaaac ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac
    16981 atgaaaatct gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta
    17041 cctcttttta ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagac
    17101 aatctctttc cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa
    17161 atagtcgata aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac
    17221 cgatttttgt taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt
    17281 aaggattgta taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt
    17341 catattccca gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat
    17401 gttctcctgt atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat
    17461 catactgtgt cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg
    17521 cagtggcata acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt
    17581 tccatgcacc ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta
    17641 cgttatcttg caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa
    17701 tccattgccc attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc
    17761 ctttaatccc aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac
    17821 tttcaacgat ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac
    17881 tttgtgcagt tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag
    17941 cataaaaagc atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg
    18001 tcaatgtttg cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct
    18061 gagcttctgc tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt
    18121 cttcggttga aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt
    18181 tttcaattcc tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt
    18241 cagctagcac tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata
    18301 aattctttgc tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg
    18361 taactaaaaa agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa
    18421 taaatttgct ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc
    18481 ttaaattgat ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt
    18541 tatggattat acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc
    18601 tggcgtttct atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc
    18661 tttttgcgct tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc
    18721 agtttgaaca gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg
    18781 acgtaatcct tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc
    18841 aggataaaca acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt
    18901 ttccatgatt ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag
    18961 catattttgc cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc
    19021 attattgtac aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg
    19081 gacttgtgca aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga
    19141 ttcttttatt gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt
    19201 ttctgctaat agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat
    19261 cactttttct gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta
    19321 tactcactat ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat
    19381 tttggcaggg attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt
    19441 cgctgcaaca atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc
    19501 gatccaagca tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg
    19561 atcggttaga tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc
    19621 taaacagatg ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc
    19681 tatactaata tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc
    19741 tactttgtca ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc
    19801 agtattcagc gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa
    19861 gagcggatcc gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat
    19921 tttgctccct ccttttatcc ttactttata acggacggct tatgatgtct aatacttata
    19981 aaacatactt aactattcct tttaactata cttagtggta tgctttttta aaaacaggag
    20041 ggaaaaaatg gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat
    20101 cagccgagca gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga
    20161 tttagaagaa gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac
    20221 ggattctggt cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac
    20281 ggcgaacaat ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga
    20341 aactaaagca atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga
    20401 tatccagttt catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg
    20461 attacttgat tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg
    20521 actgccagcc caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca
    20581 aacagtcatc catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc
    20641 agtggacagt caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg
    20701 aacatataac ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt
    20761 agctctagat gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc
    20821 gccattacaa gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc
    20881 tcgtaaattt ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc
    20941 tttcagcttt ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt
    21001 ttcttaattt gttccttcct tttatttctg ataattccag taaattacta taattataaa
    21061 aaaagaattg acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt
    21121 tcatgtgact agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag
    21181 gtttttcgtg tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa
    21241 atcttcaaaa agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg
    21301 gtagatgacg gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa
    21361 agaaatgatt tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt
    21421 caaatcaagt tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa
    21481 acgatcattg atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc
    21541 gctttgaccg accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc
    21601 aacaaacttt taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg
    21661 gcagtagagt atctgacaaa agagcttggg attccatata cttatgacga agcaggatat
    21721 atcgcaatcc atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt
    21781 gaagtgacga ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc
    21841 cattcagaac cattttcact gaattattca agactggtga atcatttacg actgcttcta
    21901 caacgaaccc atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa
    21961 cgaaaatacc cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa
    22021 taccagctcg cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta
    22081 agaagcgcaa tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat
    22141 atctacaacg aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt
    22201 tactagtagg gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt
    22261 tacaagctgg cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag
    22321 cactagggat gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg
    22381 aagttccagt aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag
    22441 aatcagttga tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat
    22501 tgattgcggc agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt
    22561 tctttagcgg caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg
    22621 cagtgctact gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca
    22681 ttttgagttt aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc
    22741 ctacaggatt gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg
    22801 atattggtaa atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg
    22861 gatttttccc aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg
    22921 cacgtccaga aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat
    22981 tctttactgg tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat
    23041 atgtcttgca tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga
    23101 cagcaggatt tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga
    23161 ttgcgaatca gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact
    23221 ttggctttaa ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag
    23281 atctttcaga tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa
    23341 gcaaaatcta cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta
    23401 cacgccttcg tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca
    23461 ctggcgtacc tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag
    23521 aagtacaatt cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg
    23581 cacaaacaaa caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata
    23641 tttacgctat cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg
    23701 cggaaaaaat gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc
    23761 caatcgctgg tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag
    23821 atgctggtat cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac
    23881 cattcacttt atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag
    23941 ttgatcttgc cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa
    24001 atggcgacaa agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata
    24061 aaatcggctc tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat
    24121 aaacaaacag gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc
    24181 gttctcctgc caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt
    24241 tttatttgac tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg
    24301 gtgttcaaaa accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat
    24361 tttcacaaaa tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca
    24421 ttattcctga tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa
    24481 tactttttct aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat
    24541 cgctaaaaac atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa
    24601 cgctgcatca cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt
    24661 ggctaattct tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact
    24721 ttctactagt acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt
    24781 tccatttcct gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa
    24841 ttcattctct acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat
    24901 cattttttca gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc
    24961 aaaacttgta taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt
    25021 atgttcggct tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt
    25081 caacgcttct ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc
    25141 ttctttcagt gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg
    25201 aaccatcttt tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac
    25261 taaataatgc atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca
    25321 tttataaatt acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga
    25381 aaggatgaca actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct
    25441 atattgaaat gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg
    25501 aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct
    25561 ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac
    25621 tagatgtcta tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa
    25681 atccaaattg cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc
    25741 tgcagaaaaa ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa
    25801 tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat
    25861 aattctctct actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt
    25921 ctccggggga caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg
    25981 ctgaagtcat gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg
    26041 aaaaatgcaa aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc
    26101 gctactttca cgacctctat attatcatgg tttattgact accggtatcc agatttctcc
    26161 catatatttc ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc
    26221 gtaatcttct acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa
    26281 ttcttcaggc gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat
    26341 gattcgggta gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt
    26401 cccgttatag ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc
    26461 atctattgtt ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc
    26521 gacaaaatcc gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg
    26581 tttttcttcg atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc
    26641 ttacataaaa ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt
    26701 ttttaactat ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc
    26761 ctctccccat tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg
    26821 aagccattct gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt
    26881 attaggaaaa atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg
    26941 attttctttt gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg
    27001 ggctccatca aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt
    27061 atgcatctct tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt
    27121 gactccttca aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt
    27181 cttttcgata cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg
    27241 tggaaaagat ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc
    27301 acgaaaagct cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt
    27361 cactcttgat ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata
    27421 gtccattagc gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat
    27481 tcctgtattt tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta
    27541 atcgatcaat tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat
    27601 catattatca aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga
    27661 aaatgttgta tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat
    27721 tatctgccta tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag
    27781 taaacaaagc ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta
    27841 caaagcaaat tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata
    27901 catcatacct cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt
    27961 tgtcagataa ataaaaaaca aaaaaagttc catctttaaa acacgctatt cttttacttt
    28021 ttctcacatt ctctaagaaa agaaaaggag aatgaatttt tgacaattcg agcaaaagtg
    28081 attatcaaat atgtaaaatc agctactttt ttcttaaacg tttctttata ctaaatataa
    28141 ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt tcctgaaaac
    28201 gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt gaactataca
    28261 agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt gatcacttgg
    28321 gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa
    28381 cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc caactactca
    28441 ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg ggagctttta
    28501 acagagattc ttatagaaca tccgcaattt atcgaacgct tcaaagttaa gatgccattt
    28561 catgcaataa attattcaga taaattaaca actatcatcc ataaatacga tcatattatc
    28621 aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa
    28681 gaagcagttg aatcgctcat tcagcaaaat gcctatcttc ccgcattcga attgatcaat
    28741 gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg aacccttctt
    28801 ctgatagagg actgttatta tttatggaaa gaattattag cagaagcacc tcatgcagaa
    28861 aaaaggcaaa tgttctcttg gttcgtctgc caagtcgatc atactgacgc ctcttattca
    28921 aaacgctact ccataaaaat cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa
    28981 aagaaaaaaa tagataaaaa aactaaagaa acggtaaaga aagacgattt caatgaaaaa
    29041 tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga ccttgcttta
    29101 aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc aatattagtc
    29161 atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct tcgttgctgg
    29221 gtagattttc cggcattgtt gatagatttt atgatacttt tctacatttt gtaagagagg
    29281 ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca
    29341 ctctaatctt taaagataaa tattaaaata acgattaata atattaacag tctttatttt
    29401 tcaccaccaa aaacctatct ttgcatatac taaatagacg tatatacaca gataggtttg
    29461 tcttgtgaaa aataatgatt atccttacca aaaaatgttt gtttttctac atgttcgtac
    29521 ttatcaaaaa atatgatcct tattgaaatt actggctatt tatattccaa cgacttcttg
    29581 tgttaatttt tgttatctca tagtaaatta tgtttcattt tcacttaatt tagttgtcac
    29641 gtgttcgtca attcgtaaaa acggtaaata tataaacaca cttgcggcta taattaatat
    29701 ttgtaaaaca gctgctcgcc agtctccggc tgttgctaaa aatcccgata taactggtgg
    29761 cgtagtccaa ggaatttgca ctactacatg attaattaat cccacagcag ttgcaaaata
    29821 tgctactgtc aaagaaaaaa tcggtgcaag cacaaaggga ataatcagac tgatattaaa
    29881 tacaataggc aatccaaaaa taataggttc gcttatattg aatatagcgg gagttaccga
    29941 taatttagca aagtccttat aatcttttct acgactgaag ataaatattg caatcaataa
    30001 agcaatcgta tttcccgatc cacccattac cgcaaaggta tctttaaagg ccatattgat
    30061 aatatgtgga atttgctctt tgtttgcata agctgccata ttttcttgca tattctgaat
    30121 caaaaacgga tctaacaatg aacctgatat tactgcttga tggataccaa atccaaaaaa
    30181 tagattagca atagatgtaa ttaacaaaaa tcctggtaaa ccagttgtaa ttttacttaa
    30241 tggctctgta actatcgtgg taattagctt atttatatcc atattaaaca attgaatgat
    30301 tataaatgac aataaagcaa acgaggtaat cgtaataatg attgggatta atacattaaa
    30361 agactttaaa acagcgggag gaatatttcc ggatagctga atctgtagtt tcttattatt
    30421 agaaagttta ataaataatt ctgtagccgc taatccaaca aatataccta caaacattcc
    30481 tgaagctcct gtataggata taggtatcac acccgtcact tcaacagctt tatccgcatt
    30541 ttcaggagta aacatcatct gcataggtgt taatacaaca aatgtcgaaa tagcaactaa
    30601 taccgctgca atatgatttg tataatttct gcttgcacac atatgataag atactgctgc
    30661 tacaactaat aatgtagtta tgcttaatgt accattggca attgaagtac caatttgttg
    30721 taacgtggtc aatgtactag tatcgacaat ttcccccatg aatcctgttg gttctaagat
    30781 cactgaatta ataagtgtca caaatcctgc taacatcata aatggcataa atgtagcaaa
    30841 cgcatctcgc aaagttctta aatggatctg attccccaac ttagtcgcaa ctataccaaa
    30901 tttttcaaat acaatgtcct tcctgctact tttgcccata ttttttctcc tttatgctta
    30961 ttgtaatcga ttactttttc cacccaaata atacatcact tatcaaaaaa agtaaatact
    31021 tttaattaaa tatatatatc ttttttagta taaaaaaata agctctgaaa aaataatttt
    31081 cccgagctta tttttcaaca aaaacagctt caaaaacaaa gtcttgatat aaatgtctcg
    31141 ataccgaata ctcgaaaggt ttaccagacg atagagcaac aactttgtct atctcaatca
    31201 taaattcacc tggatctatt tttaatattt ttgcgtcaaa ttcattcgca gtatctcctt
    31261 tgatacgtat gtgcgaagtc tttatttcca gacccagttc atttgtgata taagcatata
    31321 cagatttctt taaatgtttt ggttccaagc ctggtatgac tgctaatggc atgaatgttt
    31381 gctcaatcga gtatggttga caatcaataa aacggcttcg tacaatatta tatacatagt
    31441 cttttacact gatacttaat ttatcagcta cttttttcga tggaagttca attgagaaag
    31501 tatggatttc tgaatctacg tgttcttcac caaccacgct aaacaaacct tcgtttggac
    31561 caagaactcg tgagttatca attggtgttc tgacgacttc agtcccatac ccacttctac
    31621 ttcgcaaaac tccttcggaa acaagtaagt ctacgccttt ctttactgtt aacttgcttg
    31681 tatgataaat aactgccaat tcatctcctg taggaagttt ctcaccaatt ttatactctc
    31741 catttacaat ttttttcttt atatctaagt aaatttccat atacttcgca atttttttca
    31801 atggtgaccc tccaatcaaa aacatatctt catctatact ttaacataaa taaaccgata
    31861 aaatccacat caaaattata cacacaataa aaaagatata tatatttaaa caaaactatt
    31921 tactttaagt tgtaagcggt gttatcatta agtggaaaca ggaggtaaaa aaattatgac
    31981 aactatgaaa aagctaactt ttccagatga tttttggtgg ggttccgctt ggtcagctga
    32041 gcaagcagaa ggaaaaggag aaacaggaaa agcagagact atctgggatc gctggtttaa
    32101 agaacaacct taccggtttt atcaacgaat cgattcctct attacaaccg atcacatcca
    32161 tcaatataaa gaagatatag aactaatgaa aaaaactggg cataactcct ttcgcgtttc
    32221 tatttcttgg gcacgaatgt ttccaaatgg cggtaccgga aaagtaaatc ccaaagccat
    32281 ttctttctat cgaaaattat tttccgaaat gaataataac ggaatcaaag tatttgccaa
    32341 cttatatcat tttgacatgc ctgctactct tcaagataaa ggaggatggg aatcacgtga
    32401 agtagttgat gcttatgttc attacgctga aacttgtttt aaagaatttg gtgacttagt
    32461 ttgtcattgg tttaccttca atgaaccact tgggcctatt ttaggtactt atcttgaaga
    32521 ttttcattat ccaaacctca ttgactttaa acgcggagcg caagcagcat tcttcactat
    32581 acttgcacac gcaaaagcaa tacaagtatt taaagaacta gctcttccta gtaaaatagg
    32641 cgttattcta aatttgagtc ctacttttcc tcgtagtcaa aatcctgctg atgtgaaagc
    32701 agcagaaatc gctgatttat tttatacgcg gagcttttta gatcctatgg ttaaagggaa
    32761 tttttctaaa aagcttatcc tgttattaca agaatataat cagcttcctg aagattatac
    32821 gaaagaagat cttgtatgta tctacgaaaa taccgctcaa attcttggac taaactatta
    32881 cgagccacgt cgcgtcaaat ctcgttcttc tatggtggat acatctagtc catttttacc
    32941 agagtggttt ttcgaaccac atatcatgcc tggacgacga atgaacgaat accggggatg
    33001 ggaaatttac gaaaaaggaa tttatgattt gtgcatggat atcaagaacc attatggaaa
    33061 catcgaagca tttatctcgg agaacggaat gggcgttgcg gatgaagaac gtttcatgga
    33121 tgatacaggg caaataattg atgattatcg gattgagtat atcaaagatc atcttgctta
    33181 cttatggaag gcaataaatg aaggatgtaa cattaagggg tatcatcttt ggacatttat
    33241 tgattgctgg tcatggatca acgcctataa aaaccgatat gggctggttt cactcgacct
    33301 tactactcaa aaaagaacaa ttaaaaaaag tggcgaattt tacaagcaat taagtgatac
    33361 aaatggattt agatacgatg tagacttgtt agttgaataa tattctaaaa ttccacttga
    33421 agtgaacaca ttagaaaaca agaataatga gaaataccaa taacaaatac tttttcttca
    33481 tacttcattt atacttgtca gtataagaga ctgtgacaaa tgtagtgatc ctcagaagtt
    33541 aggtctggaa atctaacttg agaagatttt tttaaactac gtccagtctt ttttatttgt
    33601 ttaaaattga agttgacaag tcttcattaa aaatttagaa tattcacaag tttgacgaca
    33661 attatgaaaa tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag ttagatataa
    33721 aaactgcgat caaagacacg ttacctaccg tttttggtta tatcggtatt ggacttgcat
    33781 ttggtatcgt tgggaaagct gccgggtttc atccgctggt cgtcacgttg atgtccctgc
    33841 tgatctatgc tggttctgcc caatttatca cggtcagcat gcttgctagc catagtccac
    33901 tgctctccat cgttctctcg acctttctag tcaattcccg aatgattctg atgagtatga
    33961 cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt ggcacattgc
    34021 tgacagatga aagtttcgca ctgggaatga ataaattgaa ctatactgga caaaagttaa
    34081 actttcgttg gatgaataca gctaattgga tttcttattt gacgtgggtg ggttcttctc
    34141 ttgttggcgc acttttaggg aattttatta ctgatccgaa aaagtttggt ttggatttcg
    34201 ccatcgtcgc tatgtttatc gggttacttt atcttcaagt catttctgat agaaacacct
    34261 ctaaacgatt acagttgatc ctgattggtc tcactttgat tctggtatat gtcggactga
    34321 tttttatccc aagtaactta gtgattttag ttgtaacact gattgggtgc ggattggggg
    34381 tgtggatcaa acatgccttc ttttgagttt acattgctga ctattttagg ttgtacttta
    34441 gcaacttggc tttctaggat cctaccattt gtcttactga aaaaattcga tttgccgcaa
    34501 ccgttgcttg agtatttaag ttttgtcccg atcgtcatta tgtctgcttt atggttcagc
    34561 agtctattta cccaaaatat cggacatctg ccgcagatca atctcgaaaa tgctttggct
    34621 tcagttccta cacttttagc tgctattctt tcaaaaagct tattggtgat cgtcttggta
    34681 ggaattcttt ccctttcgtt gattcgctta ttataaataa aaaaacaaga gcttatggca
    34741 aattactgtc atgcgctctt gtttttatca aagtcaaata agaaaaaaat cggcgtattc
    34801 ttgcttatcc gttgaacgga ccactaacaa cgacttttcc taatatatgt cctgtttcta
    34861 cgtctttggt tgcttgtttc aagttcttcg cattcatccc atctgaatag accttgctca
    34921 atgtactgcg gataatccct tcatttgcta aagcagcaat gtgcgctaaa gcttctcctt
    34981 gcgtttcgat ttcatagtta tagtctgttt tagcgaacat atactcccag tcgaagctga
    35041 tagattggtt tttccaaaca cctatatcta acggctcttt gatccctacg atcgttccca
    35101 catgtccaaa tggtcggaca aggtgtttga tttgatcaaa gtagcgggta atatcgaata
    35161 atacagcgac atactctact tgatcgattc ctaattcacg aaaactttta cttaagtcgt
    35221 gatgataatc gattggatag tctacacctg tttttttcag ccattcaaaa ttcttcggac
    35281 ttgcagtcgc gtagacttct aggccagccc aatgcgctag ttgattcaaa atcgaaccga
    35341 cgcccccagc tccgttgatc actaagattt ttttcccgcg gttggcattt tcttctggag
    35401 tcagaccaaa tttttcaaat aacaattcat aagcggtcaa tgaggtcaac ggcaaggctg
    35461 cggcttcttc atctgaaaga ttttttggtg caagagcgac aatacgttcg tcaaccagtt
    35521 ggtattcttg attgcttcct gctcttgtcg ttgtacctgc gtaaaaaacg cgatcgccga
    35581 cagaaaattt ttgcacttca tcacctacag caacaacttt cccgactcca tcaaacccta
    35641 agatacgtaa agcatttctt atcccgttat cttggcgaag tttcgtatcg acaggattaa
    35701 cagatactgc gttgactttt accagaagat cccgtgaact gggtacaggt atctgttctt
    35761 tttcatcaat aaagcttttt ggatcatcga tcgacaaccc ttcataaaaa ccgactacct
    35821 tcattaattc cttttctgac atacgaattc ctccttagct tcatcaagtt ggagcatcca
    35881 tacagttttc atttcaatat atatagtatg ctatttgttc cacttttatt catcataatt
    35941 attctcttac ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag
    36001 acttttgtca caacctcttt taaacggtgt tcaatcaact gaccctcggt attcgctcaa
    36061 aaaacgacaa aatatgagga gctatttttg tttggctgtt cttattactt gatgcaggac
    36121 agctttttca cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt cactgaaaag
    36181 cgttggatgt tctttttcaa aggtgtctaa gatctctcgt acttcatcag tagatttcgt
    36241 attcatcaac tgaactcgca agtcatttgc tcctggaaat cctttgatat agattttaaa
    36301 gaaacgatgt agtcctgtga tggaacgcgg gatgatttct gaataatgat cttgcagatc
    36361 tagttgcaga cgaagtagtc cgatcagttc tttttctgta tgttccctcg gttctttttc
    36421 aaatgcataa ggattcttga aaatcccccg gccaatcatg atgccatcta cgccatattt
    36481 ttcggccaat tcttctcctg tctggcgatc tgggatatct ccgttgatcg tgatcagtgt
    36541 ttgcggagcg atttggtcac gtaaagcaat gattttaggg atgacttccc aatgtgcatc
    36601 tactttactc atctctttac gcgtacgcaa atggacagaa agattggcta tatcttgatg
    36661 aagtaggtga ctgatccatt cttccatctc agacatttcc gtatagccga tacgcgtctt
    36721 gacgctgaca ggcaaaccgc cagctttagc cgcttcaatc aattgtgccg ctacttctgg
    36781 gcgaaggatc aagccactgc cttttccacg ttctgctaca ttagggactg ggcagcccat
    36841 gttgatatcg attccttgga agcccatttc tgccatagca atgctcattt ctctaaaaaa
    36901 ttctggcttg tctccccaaa tatgggcaac catcggctgt tcatcttctg taaaggtcaa
    36961 tcttcctcgt acactgtctt ttccatctgg atgacagaaa ctgtctgagt ttgtaaactc
    37021 agtaaagaag acatccggtg ctgctgcatg ctttacgaca tggcgaaata caacatctgt
    37081 tacatcttcc attggtgcta aaacaaaaaa aggctttggc agctctttcc aaaaattagt
    37141 agtcatttat ttgttactct ccattctatc aagcagtgaa gtcgcaatct ttctcaaaaa
    37201 cattgtcatt atactaggaa acaaaaaaag aagcaaagtg aacagcttaa aagatgtttc
    37261 tgattgagca aaaaaagact gtgacaaaaa tcttatcaca attccttttc cggaataaat
    37321 ggtgttcaaa agcttgcatc tgcggtaata ag
//
LOCUS       DACEDM010000021        37311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEDM010000021 DACEDM010000000
VERSION     DACEDM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.021"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            129..992
                     /locus_tag="IWO50_000968"
     CDS             129..992
                     /locus_tag="IWO50_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0358105.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1121..2743)
                     /locus_tag="IWO50_000969"
     CDS             complement(1121..2743)
                     /locus_tag="IWO50_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0358106.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2992..3513
                     /locus_tag="IWO50_000970"
     CDS             2992..3513
                     /locus_tag="IWO50_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358107.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3699..5429)
                     /gene="spxB"
                     /locus_tag="IWO50_000971"
     CDS             complement(3699..5429)
                     /gene="spxB"
                     /locus_tag="IWO50_000971"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0358108.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5594..6412
                     /locus_tag="IWO50_000972"
     CDS             5594..6412
                     /locus_tag="IWO50_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0358109.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6515..6712)
                     /locus_tag="IWO50_000973"
     CDS             complement(6515..6712)
                     /locus_tag="IWO50_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358110.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6881..7459
                     /locus_tag="IWO50_000974"
     CDS             6881..7459
                     /locus_tag="IWO50_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0358111.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8504..9007)
                     /locus_tag="IWO50_000975"
     CDS             complement(8504..9007)
                     /locus_tag="IWO50_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0358112.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9366..10391
                     /locus_tag="IWO50_000976"
     CDS             9366..10391
                     /locus_tag="IWO50_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0358113.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10714..11592
                     /locus_tag="IWO50_000977"
     CDS             10714..11592
                     /locus_tag="IWO50_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358114.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12068..12184
                     /locus_tag="IWO50_000978"
     CDS             12068..12184
                     /locus_tag="IWO50_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0358115.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13112..13450
                     /locus_tag="IWO50_000979"
     CDS             13112..13450
                     /locus_tag="IWO50_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358116.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13544..13774
                     /locus_tag="IWO50_000980"
     CDS             13544..13774
                     /locus_tag="IWO50_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358117.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14174..14545
                     /locus_tag="IWO50_000981"
     CDS             14174..14545
                     /locus_tag="IWO50_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0358118.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14819..15511)
                     /locus_tag="IWO50_000982"
     CDS             complement(14819..15511)
                     /locus_tag="IWO50_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0358119.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15514..16299)
                     /locus_tag="IWO50_000983"
     CDS             complement(15514..16299)
                     /locus_tag="IWO50_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0358120.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16536..17936
                     /gene="lacG"
                     /locus_tag="IWO50_000984"
     CDS             16536..17936
                     /gene="lacG"
                     /locus_tag="IWO50_000984"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0358121.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17961..18278
                     /locus_tag="IWO50_000985"
     CDS             17961..18278
                     /locus_tag="IWO50_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0358122.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18291..19964
                     /locus_tag="IWO50_000986"
     CDS             18291..19964
                     /locus_tag="IWO50_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0358123.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19978..21609
                     /locus_tag="IWO50_000987"
     CDS             19978..21609
                     /locus_tag="IWO50_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0358124.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22100..23665)
                     /gene="guaA"
                     /locus_tag="IWO50_000988"
     CDS             complement(22100..23665)
                     /gene="guaA"
                     /locus_tag="IWO50_000988"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0358125.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23724..24647)
                     /locus_tag="IWO50_000989"
     CDS             complement(23724..24647)
                     /locus_tag="IWO50_000989"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0358126.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24843..25448
                     /locus_tag="IWO50_000990"
     CDS             24843..25448
                     /locus_tag="IWO50_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0358127.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25756..26772
                     /locus_tag="IWO50_000991"
     CDS             25756..26772
                     /locus_tag="IWO50_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0358128.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26913..28214
                     /locus_tag="IWO50_000992"
     CDS             26913..28214
                     /locus_tag="IWO50_000992"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0358129.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28260..28922
                     /gene="deoC"
                     /locus_tag="IWO50_000993"
     CDS             28260..28922
                     /gene="deoC"
                     /locus_tag="IWO50_000993"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0358130.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28943..29341
                     /locus_tag="IWO50_000994"
     CDS             28943..29341
                     /locus_tag="IWO50_000994"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0358131.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29407..30489
                     /locus_tag="IWO50_000995"
     CDS             29407..30489
                     /locus_tag="IWO50_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0358132.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30701..32263
                     /locus_tag="IWO50_000996"
     CDS             30701..32263
                     /locus_tag="IWO50_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358133.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32256..33389
                     /locus_tag="IWO50_000997"
     CDS             32256..33389
                     /locus_tag="IWO50_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358134.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33389..34345
                     /locus_tag="IWO50_000998"
     CDS             33389..34345
                     /locus_tag="IWO50_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358135.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34444..35610
                     /gene="deoB"
                     /locus_tag="IWO50_000999"
     CDS             34444..35610
                     /gene="deoB"
                     /locus_tag="IWO50_000999"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0358136.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35634..36452
                     /locus_tag="IWO50_001000"
     CDS             35634..36452
                     /locus_tag="IWO50_001000"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0358137.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36469..37176
                     /gene="deoD"
                     /locus_tag="IWO50_001001"
     CDS             36469..37176
                     /gene="deoD"
                     /locus_tag="IWO50_001001"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0358138.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11634 a         6517 c         7892 g        11268 t
ORIGIN      
        1 ccacttctgt cacaacctct tattttgatt agaacttgcg aaaataaacc ggttaaagtg
       61 tgtctagcgc aagagatttg ctataataga cgataagggt aaaatgtaac gagaaggggt
      121 tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga tctgccatac catgtattag
      181 caaaagaaaa agttgatttt ttatcaatga atctccaatt gaatgatcaa gagttagtag
      241 atgacctcgg aaagacattc aattatgacc gctacatgaa tcagctgaaa aatggtgcga
      301 tgcctacgac atcacaaatc aatgttggcc gatatttaga atttttccgt tcttatgcgg
      361 aaaaacagat tcctgtttta tatgtggctt tttccagtgg attaagcggc tcttacagta
      421 gtgcgcttca agcggttgaa ttgctgaagg aagaatatga agaccctgag atctatgttt
      481 ttgatacaaa agctgcaagt attggggaag ggcttttagt attggaagct gtaagattga
      541 aaaaagaagg taaaagcatc gaagaaaccc tagagtggct ggaaagtaat tatttgaaag
      601 tccattcatg ggtaacagta gacgatctga aatatctaga acgaggcggt aggatttcca
      661 aagcaagtgc tactcttggc agtctattga gtgtaaagcc tattattacg gtggataaag
      721 aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa caagtcgatc cagacaatcg
      781 tcaatgagac ggtaaaaaca atcgttgcac cacttgatca aatcgtttat gtggcttacg
      841 ctggggatct agagagcgcg gagaaggcaa aacggctttt ggaagagcag ataaagatga
      901 aagatgtgaa gctttatccc ttaggaccaa cgatcgccag tcacacaggc tatggctgta
      961 tcgcgatttt ttcgatggga atcagtcgat aaaagcagta actcattctg taaaaaagcc
     1021 cagaaattga ttgtaacaaa aacagcgact cttaaaagat agatgagcat cctatctctt
     1081 agaggtcgct gtttttttat tttttaagct aaataggcca ttatgcccat aactcgttca
     1141 attgtttttg aatatctgca ttttccaaaa attcatcata tgtcgtatca gctcgatcga
     1201 tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa tgtttggata aattgatggt
     1261 catgtgacgc aaataagatt gagccagtaa atgccatcaa gccgtcattc aatgctgtga
     1321 ttgattctaa gtctaaatgg ttcgttggat catctaatac aagtacgtta gctttcgaaa
     1381 gcatcatttt tgaaagcatg caacggactt tctctccacc agataaaaca ttcactggtt
     1441 tcaatacttc ttcaccagaa aacaacattc ttcctaagaa gcttcttaag aaggtgttat
     1501 catcttcttc tttacttgca tattgacgca accagtctaa aatcgttaga tcagtatcga
     1561 attctttcgt tgtatccttt ggtaaataag aattacttgt tgtcacaccc caacgtactg
     1621 ttccagtatc tggcgtaatt tcacccatga taactttgaa tagcgttgtt gttgttatat
     1681 cattttctgc gacgaaagcg accttgtctt ctttgctcaa attaaaagat atatcatcta
     1741 aaattttctt tccatcaatc gttacactta cattttctac ctgcaataag tcgtttccaa
     1801 tctcacgttc aggtttgaat ccaacgaatg gataacgtct agaagaaggt tggatatcgt
     1861 ctaaagtaat tttttcaagc atttttttac gtgaagttgc ttgttttgat tttgatgcat
     1921 tcgcactaaa gcgggcaata aattcttgga gttctttgat ttgttcttct tttttggcat
     1981 ttgcgtttgc ttgaagttta gccgctagtt gactagattc caaccaaaaa tcataatttc
     2041 ctacataaag tttgatttta ccaaaatcta gatccgccat atgtgtacat actttgttca
     2101 aaaagtgacg gtcatgggat acaacgatca cagtattttc aaaattgatc aaaaattctt
     2161 ccagccaatt gatcgattgg atatccagac cgtttgttgg ttcgtctagc aaaagaacat
     2221 caggtttacc aaatagtgtt tgtgctaata acacttttac cttttgtcca ccagttaatt
     2281 cactcatttg ctgatggtgc aattcttcag ggatgttcag accttgcagt aaaacagctg
     2341 cttctggttc agcttcccat ccattaagct cagcaaattc tccttctaat tcagctgctc
     2401 gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat cgcatctttt tctttcagca
     2461 cttcatacag acgcttgtgt cccatgatga ctgtttcaag tacagtatag tcttcatagt
     2521 cgtaatggtt ctgtttcaat acggccatcc gttcatctgg tcccatagta acggaacctg
     2581 tggaaggctg cagatcccct gataacactt taaggaacgt tgatttacct gcaccatttg
     2641 caccaatcaa gccatagcag tttccggggg taaatttaat attcacatca tcaaatagtt
     2701 tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat caattttttt cctcactttt
     2761 atcattatta gttttgacaa gtcttataga attatatagg ggaaaggtaa aagattcaag
     2821 gcaatcgaac attctttgtg tttcacgtga aacagaatga aaaaaataaa aataaaagcg
     2881 gagcgaaaat agctactata ggatttatta gtttgcaaca gcataatttt tgacgttatt
     2941 tataagaggg agtactataa aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa
     3001 aaaagaacca tcgactgggg ctcattgatt ttgggaattt tatttgtact tgtctcattg
     3061 ttatcatttc aagaccctgt cggaaattta gttgcaattg tagtagtctt tgcgattttt
     3121 gcctttatta aagggatatt tgaactgttt gtacggaaca gattaaaaga gctaacagga
     3181 tataaaggaa agatgccctt aataatcggg atcatcgata ttctagttgg cgtctttttc
     3241 ttattcaata ttggtgcagg agtcgcagca ttgccttttg tttttgcagt atggtttatt
     3301 gctgattcag ttttagcgtt atttactgct gacttggcaa gaggagtaag tgaaggatat
     3361 tattggttta cgatcatcgt caatatttta ggaattttat taggaatttt cttattgttt
     3421 aatccaattt cttctgcgtt gacattaagc tttttagttg gattctattt tatgttattt
     3481 ggtatcacac atatcgtcta tgcatttaga taagtaaaat caaacaaata aaaaagccgg
     3541 cttccgatta cagttttact gttgtaattg gaagccggct tttttatcat ggacaaatat
     3601 gcacgaggtg atttttaggt gtcgagacaa atttatgcaa aaatgtgaga agctatatac
     3661 acaaatttta tcttaatact cgaagcatag cgcagttgtc acaacctctt ttcatataat
     3721 tcattcaata ctggcaaatc atggacttca tatttttctt tgaatgcttg gatttcttct
     3781 tcagaatatt ttcttggatc taaatgcaga tcttctactg gcaatgggcg cttgttattg
     3841 atcttcacat cgattactac aggacgatct gattttgcag ctgcatcaaa ggcaaatcga
     3901 agttgcgagt attctgtgac tgtaaagcta tttgccccga gcgcttctcc aactttaccg
     3961 aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct
     4021 tcgatgaaac caaaagaatc attcgaaaag acaacattaa taattggaag ctgatatttg
     4081 acttgtgtaa tgatatcttg catgaccata gcaaacccgc catcaccact taaggtaaat
     4141 acttgttttt ctgggtagct tagttgtgcg gcaattcctc ctggcacacc attacccata
     4201 gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt tcatattcaa taaacgaata
     4261 gaatggatag tcgtgttacc gacatctgtg acgaaaatcg catcgtcttc agcaatacga
     4321 ttgatttctt tatagacagc ctctggacga atcggttctt cctctcgatc ttcaaaagat
     4381 ttcaaccaat ttacccattt ttctttattt tcttgatttg ctaggtacca gctatcagca
     4441 gggcgagcat cccctaactc gacaagtctt tgcaaagctg tgtttgcatc accgaggatc
     4501 gacaaagatg tttcatggcg tcgtccaaat tttgacgcat cgatatcgat ttggataaat
     4561 tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt cgctaccgac aaataagatc
     4621 agatcggctt ctgccaatgc ttcatttccg ggtttagtag cgacacgtcc agcaaaacca
     4681 agaaagtttt cataatcatc aggaataatt ccttttgcta gaacggaagc gacaacagga
     4741 actgaaaaat gttctgagaa ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca
     4801 acataaagaa ctggtttttt tgctttttca agataaggca aagcagctag caaatctttt
     4861 tcttctggta atatcacact tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca
     4921 atttcttcaa aaccaagatc aacagggata gtcacgatag ccacgccttt atgctcatag
     4981 gcggctttaa tggcttcatc tactacatgc ggaagacttt gtggtgtcat gactgtacga
     5041 ttgtatacgc taacatctgc gaaaatagga ttttcattta gttcctggaa agagttatag
     5101 ttcatggatg tagaagcaac ttgtcctaag aacgctacta gaggcacatg gtccatctgt
     5161 gcatcataca aaccattgat caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg
     5221 ccgactttcc ccgttaattt tgcatctgca gcggctgcga gtgcgccgac ttcttcatga
     5281 cgaacttgaa tgtagttgat ttcggttttt tcttgatata gcgcatccat aattgaattg
     5341 aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc agctttcaag aacttttacc
     5401 attgcgattc ctgcattgat ttttgacatg gtgtttcact cctttagcaa tgatgataca
     5461 taaatttttt gtatctagag tatagaactt tttatcgaaa atcccaacta attcgtcttt
     5521 tcacaaaaat gtagtcatgt ttttgggaat atgctagagt agtacataga tagattcaag
     5581 agaggggaat ggcatggttg aatggctatt gagacttgaa ccttggcagc aagcacttgc
     5641 tgggacagct tttacttatt ttatgacagc tcttggtgcc gggttggttt ttttctttaa
     5701 agaaatcaaa aaagaagtgc taaacttgat gttaggattt gcttctggtg tcatgattgc
     5761 tgcaagtttt tggtctttgt tagatccggc tattacaaaa gcagaagaaa atggagatat
     5821 tgcttggcta gttgtaagta ttgggtttgg tttaggcggt gtgtttttat atatggcaga
     5881 taaaacactg ccccatatgc attttggacc acaacatgaa gcagaaggat tgcctaccca
     5941 tttaaaacgg acgatcttgc ttgttttttc tattacattg cataacattc ctgaaggttt
     6001 agccgtgggg gtagcattcg gtgcagcagc aactgcggat aatccgacag ccgctatatt
     6061 agccgctgtt tcggtggcgc taggtattgg tatccaaaac ttcccagaag gagcagctgt
     6121 atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag gcctttgttt acggacaagc
     6181 ttctggaatc gttgaaccaa ttgctggagt gattggagcg ctactagtaa cgaaagtgga
     6241 gctgctgctt ccttatgcac ttgcttttgc cgcaggagca atgatttatg tcgtggtgga
     6301 agaattgatc cctgaagcac agcagacatt gtccagcaaa cgtcattttg cagtttttgg
     6361 cgtcatgagt ggatttatca ttatgatgat tttggatgtg gcattaggat aaataaaaaa
     6421 aatatatatg atatatcaaa tagggccgcg aacttatatc gcgaccctat ttttttagta
     6481 agttatatca taatgaagtg aatggtatca tacattaata atagtttttt ctatgtgtat
     6541 ttcctttgat ccagcgaaca agccagatga taagtgcgat tgggattaga actttgaccg
     6601 caaaccataa aagcccgctt aggacactga caaaaagact gatgacgaag ctagctgcaa
     6661 ttagcactaa aagaaccatg aagatattta agaacatatt tttttgacgc atacaaaacc
     6721 ctcctcttgt tcattcttta ctatagaaaa agtataacag ggattgttta aaaacacctc
     6781 caactgaaga ctgattttta cttcatctac ttaaatgatc aaacaattgc tttttctctg
     6841 gtttatcatc taaaatagag cggaaaagga ggagaatatc atgcctaaaa atcgtcttga
     6901 agcatttaca gatgctgtaa tcgcgattat catgactatt ttagttttag aacttcatga
     6961 acctaacgga gacacgtggg aggcgctcgc tgcattaggt taccgatttt ttatctatat
     7021 tgtcagtttt atcattttag cgatttattg gaacaatcat catcatttat ttcaagttgt
     7081 acataagata aatggacggg tcttatgggc gaacagtttc tttgtttttg cgctttcgct
     7141 ttttccgttt gcaacttcat ggatcagtga gtacatcaat agcctggctc cagaaatgac
     7201 ttatggtttt gttaccttga ccgctaatat tgcgtattat atcttggcaa aagaacttgt
     7261 caaagcaaat aaaggccacg agaaagcatt gaaggcgatg ttgaataatt acaagaaatc
     7321 gtatctatcg attggtttga atgtgttagg aattgtctta gggtatttag tagcgccagt
     7381 agccgtgctg atcgcaaaca tcgtgattct agctgcttgg atcatcccaa gtcgtaccat
     7441 cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt aagagaaaaa tatagaaaaa
     7501 ataaataaac gatcaaatat ccgagtgata ctagtattca aagagggatg cagatggatc
     7561 aaaaaagaat tcatgtagga ctgattacta gttttgtcac gatgcttatt tatttggatc
     7621 aaggtcattt gacttggaca gtagttggtg tcgtaggatt tttgacatta ttcacagcaa
     7681 taaaaaaata aacatcttta tgtaatctga tgttctatct atttaagaaa agcatgttta
     7741 cagcagtatg attctgttgt aaacgtgttt ttatttgtgg aaaatttaat ttttagaaaa
     7801 gtgtatcgaa aaatagatag ttagtttttc cacagtggat aacttcgtaa gagataaaaa
     7861 taaaaaatta gtcaaagaag ggcgtataga tttcttttga taggagatat acaacagaaa
     7921 tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt gtggtttttg gaataaaaaa
     7981 ataacatatc tcattttaca cttttgttat tttatactta aaaaataacg acacagaaaa
     8041 taccgtgtct cagatttctc tactacttct aaaaactact gtttttttcc tttgaaatgc
     8101 cttcaaaaag taggatcagg cagaatatac tggcagaatc aaggagaagg gagcctaaat
     8161 aaggagaaaa accatagaaa atataaagta attacttata aaacaagctt tttatagaaa
     8221 aataatatac ctgagtaggg tataaattaa gacggaattc ctttctagat tgagatattg
     8281 actaggaaga atgttaagat tttaagcctt taagctaaaa aaaaaccctt tttgttaaat
     8341 aaggggaata tagcctgtca gtttcaaact gcaagtcgtc ctgctgcaat aatatagtag
     8401 attagagatg aaaatagatt tttttaaacc aatgactgaa taaaaagacc gtcatagata
     8461 cacattggaa tgtgtctatt acggtctttt tttacgaacg attttattga agacggacag
     8521 ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa taaaatgtat cccaaaataa
     8581 tggctagacg gtctaactta tttgtcgata gatgtttgtt tagaagttga taggcccata
     8641 aacctatcag tcctcctgtt gtattagtta tcaaatcagt gatgtccgct acgccaatac
     8701 cgaataggaa ttgaatcaat tctgccaaga agcttacaag gaaaataatc cctaattttt
     8761 tagaaaaaga aagtctcttg aaattgacat tcaaaagcac cccaaaagga aaaaagacaa
     8821 tcaagttata agtggtctca cgtaataccg tcgtattttc aaaagaaaaa gtagaaaaag
     8881 ggacgagatt gacattgctg taagaataag tgagaaattg tggaagatta aatgatagct
     8941 tgaataaaat caaccataat agaaagaata aataaatccc taataaaaat tttgatacat
     9001 accgcataga catctcctcc atttaaaaag tataccatcg aaaaccgctg aaaaatcata
     9061 aaaaagtatg aagtttaagg accaacgtag ttatcgtacg aaaattagaa gctttttttt
     9121 gttgggaatc cggtttcttt aacaaagcta agatagcgag ttgcttggaa aagttgctat
     9181 cttttttctg tcaaaacgac tcggaatgtt cgttttttgt tctaatttct ccaagttgtt
     9241 tactttttga tactaagttt atgaaaaatc aagaaatagt tgttatacta ggtaatgctg
     9301 acaaacggga ttggcatttc atcttggttc agcttttttt ccaattacta ggaaggaggg
     9361 atagattgtt caagacttat tttcgtttca ataaaataca agataatcct tttcgtatca
     9421 tgaatctgat gctacttatc tttattgtat tatggttagg ttacataaat catgattttt
     9481 tgatttcaag tttttcatca ttagggatct ttactttttt ctattatcaa aacataccga
     9541 tgaaatattt gctgaaacgt atgtttctga tcggttgcgg actgttggtt gctttcgttt
     9601 tggggatctt atcgacttat gttttatggc ttgaaccatt tgccgtagct ttggttgctt
     9661 tttccagcag attcgtgttg cgattattcc atatttctaa accaggtggc ttgttttttg
     9721 cgatgctttc tgccatggga acgagcatgc agctgccaat cgctcaatta ccgatcgtct
     9781 cactttattt ttttatgggt gtcgtttttg ccctgattgc ggcagtgatt acaaaattac
     9841 tggattcacg gcctgagcag acaattgaaa aagcaacttt aaaagaaaga tttcacgaag
     9901 agcctttagt gatcattgat tctgttttct acagcgctgc cttatttctt tccgtttatg
     9961 tcagtcacgg cttgaatctg cataatcctt attggttgac cttgtcatgt gcatcgattt
    10021 tgctagcaga aaacttggat gcgatgaagc atcgacaagt ccaatatctg attggatcga
    10081 tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc gtttacgcag ttgcagacca
    10141 tttttctgat cacttttcta tatggaatcg cacaatttct agttgctaga aattatgcag
    10201 ttgctaatat ttttcttaat ccgatggcgt tgatgctatc tacacttatt cgtggagcat
    10261 acttgatttc tttgatcgaa tatcgttttt tagggatcgt cataggaagt ttcatcggtt
    10321 taggtgttgc gtgggtaatg acggttggtt tgcaacatta tttaacggtt gttcgtcaaa
    10381 aattagaata gaaggacaaa tatatgtgcc aaaagaggtt atgacaagat ttttgtcaca
    10441 acctctaatt caaggtgttc aaaagccaga ttctcagcat taagtcaaat tcacacaaaa
    10501 atgtggaaag gaactttcaa aaaaatttta tatttattct atcaaaaaat tttgtttatg
    10561 atggtaactg aataaacttt cctgagatgg tcaatgcttt ccttgaaagt cttacagata
    10621 ttctttcttc atagaaagtt taagttttat agttagtgta caataaaagt atgtttatac
    10681 catatggtgt ccggataatc agtcatttaa acaatgaggt tcaaagatat gaaaagggga
    10741 aatttgatga agaaaataag caatgaaatc caaattctgg cagaaacttt aactcttccc
    10801 aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca tccttgatga ggtgtttgag
    10861 caatatagtc atgaactaat ggatcctaat aaagcaaagg cgactgcaaa gaaaatagac
    10921 aaagtctatg aagaggaacc ttttgcagca ttagctattt atctatatgc agcttctcaa
    10981 agttggaaaa aaatttatca accattgaag attccaagaa ctatctattt ggcaacaatg
    11041 aatgctttta ctagatttat tcatgaacat ttccaagtaa caggaagcta tcgctttgac
    11101 cgtggttttt ggatttggca ttacactagt ggcttgatct ttcggattgg tgaattggag
    11161 tacgaaatga catattttcc gaaagggtac acggatctaa gattagaagg aaagcagtgc
    11221 ctttctcttc atattccttc cgatgcagat ttatctcaga ataagataag agatagttac
    11281 gaaagtgcca ttcgtttttt tgaactttat tttcctgatt accattacca agtgatgtac
    11341 acagatactt ggttactatc acccaatctt actaaatggt tgaaaaaaga gtcaaaaatc
    11401 tgtctttttg cagccgatta tcgattatta tcagttgatg aacaggacga tagtggagtc
    11461 ccttggatct ttggacgagt agatgcccaa atacatgatt atccagaaag tacgagttta
    11521 cagcgacaag caaaagagca attgcttgca ggcgagcaca ttggctctgg cttaggtatt
    11581 cttacttcat aagtttagta cttcgaaata gtttaaaaga gcgtgagaca aaagtaaaga
    11641 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
    11701 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct
    11761 tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt ctatcgaaca cacagtacgt
    11821 tcaataggta aactagctat aacaatagat attaaagtta acggacaaga gggccttttt
    11881 taggtagcta aaacgtaata atgaaaattt atttccagag ataggagtca atcagctagt
    11941 ctgatcgact gttttaacaa gttgactttc attttaactt ttgataccat ttaaaacaca
    12001 aatcgtaatt gtttcgattt gtgtttttta gcatgactac aatcaaagaa gaaagtgggt
    12061 cgtccatatg gaaaaaacag atatctcaag tgcataccgc cgcttgaaaa gcccaaatat
    12121 caaaacacga aaacgtgcgc tgaagatcat caaagatgtg aaacggaata gtgggaaaag
    12181 gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa cgtttttcgg ctattttcaa
    12241 taatcggttt ttgtgtaagg tttccgaaag aactgctgtt gattttccat ttttaacata
    12301 tgataaattg gtagaatctt tcttttcaaa ttgataccat tccctaattc cagttatata
    12361 gttaactaca gaaccacaaa gcacgtacgt ttataaaagt attatctgta gttatttttc
    12421 aattcccttt caacctctta ttttgaccag tagtattgat cactttacct tttaacacct
    12481 ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg taactacata atcaaaggtt
    12541 ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc aatacggttg caaataacca
    12601 ttatcgttta ataagcagtt acgaaagctc ccaatatctc taacgttcag ctcactccat
    12661 ttcttttctt caaaaaaatc agtgattcgt atctctttct atttgtcgaa aaagttaact
    12721 ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa agcagttact attgctagaa
    12781 taacatcaag gaataataag ttttggtttt cagataatag tcgataagtt gtgtattgga
    12841 attttagata cagtaataaa gtatttttca tgaaattttg tgagaatcac tattacaagt
    12901 atctttaata aaattaatgg ttaaggagga acttaaagta gaatgtaaat gaatagttgt
    12961 agtcagtcga ttgtgatact agactcaaag ttgcaggaat aagtttttca atttgtgatt
    13021 ggaagataaa aaatgaaaaa gaagggcttt tttttatact ttgttgattg tagtgtttat
    13081 tttccctctg tttagccaag taacattttc tatgactcaa ccagtttcaa gtgaggaaca
    13141 tgataacctt tttcgtgcta cagaagagtt taagaaatat caggaaaata ttgttccgga
    13201 gactccatta gctagtgagc caattactga tattgatgga aaccaaatag gatggtatat
    13261 acagtacgaa gttatggtgg taaataatga ctcaatagta aaaaatacag taggcttaga
    13321 gctaacaccg attgaaaagg tgagttcatt gttaacgtat atttatactg atgaaagtga
    13381 cgagactgag atcattttaa cggattactc aaaaattgct actgataaaa agtatatatt
    13441 gtggatgtag acactagtga aatactttat gatatagaaa ttgatgaaaa tttactagat
    13501 gtagcacaag aaatcgatca tcaaaagcag gaagctattc gatatgctgg tagaaaaggg
    13561 aaaagaaaat atttattacg tgaatgtagc aaaagttaga gaagatgaaa atgagtggaa
    13621 agaattcaag agtcgctact caattaactc gactccgaca tttacagttt acagagaagg
    13681 atcgatagaa aaaactgttt tttggactaa agaaagtgga atgtctttag ctgaagtaga
    13741 agagtttttg gattatgtta gtatgcaaca gtaagggagt tcttattatg aaaaattttg
    13801 acacatatta tgttattgtt ttttcgatat ctttgctgtt aatattacta actttaatgc
    13861 agtataaaga gtatccagag tattttattt taacttttgt atatttagta agtagctaca
    13921 cacttctata taaattcaga agttcttcta gagtttctaa agtttgtttc ttaattagct
    13981 tattgattct ataatatttt tgatttatat aggaaattaa aaatattata gaatcaacac
    14041 tcagagaacg ggttggtttt taatagattt aataagtact gataatgaaa tttctcctgg
    14101 ttgggaaaac gaagttggtt accctgtaga agaatttttt caagattact ttgcggagta
    14161 tgccgatagg ataatgagag attacgtaac agataaaact caagaagtca tcaatcaatc
    14221 ccagtcatta aatgaattat ctgataaggc gaaagaagaa gcagttgcca ttatttcttc
    14281 agggattagt gactatattg atatgagtgg tgaaatcaat tatggcgata aagacaaaat
    14341 tgtacgcatg ataaaagctt ttatcaaaac ttttgatgtg aaggcagatc ttaagtttaa
    14401 tgatacttac ctagtaggaa aattaattaa tatccttggg acagagaaag atacggaaac
    14461 gtttatttcc tctttgtcta tgttgtttac agataagcat ttttcaactc agcttggtgg
    14521 agaggaacta gtggaaatct tttaaccatt acgcccagca ttaataaaag tatatcaaaa
    14581 aagtactcct gatgattttt aagattggtt tgagaaataa tgtgaaaaca ctatttttaa
    14641 tgttaattat agatagagat gaacgcgtca tatggttaaa agaatgaaaa agtcaaagat
    14701 acactgctat atttcactaa ggatatagca gtgtatcttt catgtcgttt cactggcagt
    14761 tttttataaa aggaggtgtg catttcagtt gcttttcttt ggtagttagt tgaatcgttt
    14821 aagaattaac ggatgtggca gacaggtgct ctgtccaaaa accagtaaaa tctttgatgg
    14881 ttccatcttt atttagctgt gcctgttcaa atactgtttt gtttttaccg aatggatcaa
    14941 ttgcaataat tgttccgatc cccgcacgtt tggcagcaag taatcctgag taagcatctt
    15001 cgataacaag acaatcagcc ggagctaagc tcagtttgtc tgccgcttta agaaaaatat
    15061 ccggttgcgg tttcccaggg aaagagccgt cgtcatagac aatcttgtcc cagtcaaacc
    15121 agcgggctaa atcgaaaata tcaaaataaa aagcaacatt ctctttgaca gtagccgtgg
    15181 caatcgttaa tggaatctgt tggtcagtca gctggtcaag agtcgcttca agcccttttg
    15241 tcaactgaag ctcgttttca ttttgcaagc aaagcatacg ataatagctt tctttttctt
    15301 ctgacaatac ggcgatctca gaggcggtca aatgctctga aatgaaatgt ttcaaaatct
    15361 ccgtattcgt ccgaccatga atatttttta aaatatcctc gtctgataaa ccatcttttg
    15421 tatgatcatg tatcatatgc agccaagcag cttcatgcag atgagagtcc aaaaacattg
    15481 ttccgttgaa atcaaatata atccctttca tagttacgct cctatagtgg tattttcggt
    15541 tgaatcagca atttgcagat cgacatattg tgcatagtgc tcttgaacct cccgcgagat
    15601 ctggcgatcc gtaatgagca gatcgaaatc atgcaaatta taaaacacat aaaaatcatc
    15661 tcgattgaat tttttattat caacgagtag atatttctta cgggaattat ttagagcgat
    15721 ctgctgtgct tctccttctt cggtactgta agtagagatc gcttcgttgt gaacgccatt
    15781 tgaactgata aatgcttttg taaactttag tcttcttaaa ttgtcgttgg tgatcggtcc
    15841 gacaaatgcg ccggtatttt tccggaaatc accgccaatc agtagaattt ctggtccatc
    15901 tgcttgagca agaatatcaa aaactggata attatttgta atgatccgaa ttttcttgtt
    15961 cagtaaatgc gtcgccaata gttcgattgt tgtacctggt cctaagaaaa tcgtttctcc
    16021 ctcgtgaatc aaggaggctg ctgtggcagc aatttctttt ttttcttgaa tctgcagagt
    16081 ggctttttca gtgtgagaaa gttcttgatc taaagtgtag gaaagacttt gagcccctcc
    16141 atggacacgc agcagcttgc ccgatttttc taattcatct aaatctctgc gaacggtcat
    16201 atcagaaaca tctaattgat tgatgatttc attaacggta aggattcctt ttttattgac
    16261 catttctacg atagtaagca atctttcttt tttcaacatt gtcttcacct ctaatttaag
    16321 gataatctat catataaacg gacaacagtc aatctaattc gaacaaaagt aaacatttag
    16381 ggtggttttt atgtttgtta caatagattt tattgctgtt ttctggtttt tatgattatt
    16441 tgtgtttgtt tttgttgaca aatgttttgg atgcgtttac aatgcaatca gttagaaaga
    16501 gcgaaaactg attatagatt tgaggagaga tgtacatgct taaattacca gaagatttta
    16561 tttttggcgg agccacagca gcctatcagg ttgaaggagc cactaaggaa ggtggcaaag
    16621 gcgctgttgc ttgggatgac tttttagagg aacaagggcg attcagtccc gatccggcaa
    16681 gtgattttta tcatcaatat gcaaaggaca ttgaattgtg tgaacgtttt ggtgtaaatg
    16741 gtctaagatt atccattgct tggagccgca tttttcctga tggagctggg aaaccaaatc
    16801 cagaaggaat cgctttttat catcgtgtgt tcgaagaatg taagaaaaga aacgtaacgc
    16861 cgtttgtgac gttgcatcat tttgataccc ctaaacgttt gtttgaccat ggagattttt
    16921 tgaatcgaga aacaatagaa gcatttgttt cctatgccat tttttgtttt catgaattca
    16981 aagaagtcaa ggtgtggagt acctttaatg aaatctatcc agttgcaacc aatcaatatt
    17041 tgttaggtgt tttcccacca ggaatcaaat atgattttac aaagatcgtc gcctgtcttc
    17101 ataatatgat ggttgctcat gcccgtgtag tgaactattt caaagaaaac gaacttccgg
    17161 gtgaaattgg tgtagttcac tcattagaga cgaaatatgc ggcaacagac gcgccggaag
    17221 ataagcatgc tgccttttta gacgatgcat tgtcgatccg tttcttatta gatgctacct
    17281 atttaggcta ttattcaact gaaaccttga ctgctcttga tgagatatgt gaagcaaatc
    17341 aggccagcta tcattttcct gaagaagatt tcgttgaatt gaaaaaagca tccacgcgta
    17401 atgactactt agggatcaat cactaccaat gccattttgt caaagcttac gatggagaaa
    17461 atgcaatcca tcataacggt acgggtgaaa aagggacctc tgtttataaa gtgaagggaa
    17521 tcggagaacg tatttataaa gaaggaattc ctcgaactga ttgggactgg ctgatttatc
    17581 cagaaggtct atatgacttg ttgctgcgaa ttaaaagcga ttacccacat tacaacaaaa
    17641 tctatatcac tgagaacggg atggggtata aagatcagtt tgaagatggc attattatgg
    17701 atcagccgag aatcgactat ttgagagtct atttggaatc cttaagcaag gcgataacag
    17761 ccggggtaaa tgtgaagggg tattttttgt ggtcgcttat ggatcttttc tcatggacaa
    17821 atgggtataa caaaaggtat ggcttgtttt atgttgattt tgaaacccag aagcgttacc
    17881 caaaagaatc agcttactgg tacaagttgg tcagtgaaac caaaacgatt atttaataag
    17941 tgataatgga ggaagaaaag atgaataaag aggaattaca aatgcttggt tttgagatcg
    18001 ttgcatattc aggcgacgct cgcagtacat tattgaaatt attgaaagaa gtaagacaag
    18061 gaaactttga acatgttgat tcggcgttga aagaagccga cgaaaattta acccttgcac
    18121 ataattcgca aacaaaaatt ttagcggaag aagcatctgg gaaagagatg gagatgggat
    18181 tcatttttat ccatgggcag gatcatttaa tgaccacttt attgctgaga gatctgattc
    18241 aagactttat tgtactgtac agacagaatc aaggatagga gaagaacgct atggatggaa
    18301 tcattaaaca aatcgaaaaa gggaaaccat tttttgaaaa aatctcacgt aatagttatt
    18361 taggagccgt tcgtgacgga tttttaacag cgatgccagc tattttattt tcaagcattt
    18421 tcattttgat tgctgcggtt cctgagattt ttggttacca atggcctgaa tctgtctcca
    18481 catggctatg gaaagtctat aattattcga tgggtgttgt agggttactc gtttcagcaa
    18541 ccactgctcg ctgcttggct gattcgatga atcgtcgctt accgaaaaac aaagtaatca
    18601 acaccacctc tgtgatgctg gcctctgtgg taagtttcct gttgttaagc gtctctccga
    18661 ttgaaggtgg attttcaacg gaatatatgg ggacaaaggg actactggct tcctttgtcg
    18721 ctgcattttt aacagttaat ttatataagt tttgtgtgct gaaagatatt accattaaaa
    18781 tgcctaaaga agttcctgga acgatttcgc aaaccttccg agatattttt cctttctctt
    18841 tttcagtatt tgcggccgtt ttaattgatt taggcattcg acaaatcttc caaatctcat
    18901 ttgcagaagc aatcattacg gtgctgcagc cgctatttac agcagctgac gggtacttag
    18961 gaatcgctat tatctgggga gcgatggcgc tgttttggtt tgtcggtgtt catggaccgt
    19021 cgatcgtgga gccggcaatt gcggcaatta tctatgccaa tgtggaaacc aatctgcaat
    19081 tgttcaattc cggtgaacaa gcttctaaag tactgacggt gggtttaggg aattttgtag
    19141 gaactatggg aggaactggg gcaacattgg ttgttccttt tatcttccta ctctttgcta
    19201 agtctaagca gttgaaggca gttgggaaag cttcctttat tccagtaagt ttcgctgtca
    19261 acgaaccatt gctatttgcg gcacccatga ttttgaaccc ttattttttt attccatttt
    19321 tgttaacacc tgttgtaaac gtttgcctgt tcaagttttt tgtcgatgta ttgggcatga
    19381 acagctttat gtacgttttg ccttgggcaa ctccagcacc gatcggtttg gttctaggaa
    19441 cgggcatggg tatcttagct tttgttctag cagcggttct aatcgtcgtt gacttcctga
    19501 tctatctacc attttgcaaa gcatacgatg ctgtcttagt agagaaagaa gcacaaaaag
    19561 cagaggtgat cgcaaccgag gaaggtgctt ctgccgatgt acgtgaggaa gtacctgtgg
    19621 cagcgagtca agttatcagc gatgcaaata aagaaatcaa tgtgttggta ctttgtgctg
    19681 gcgcagggac aagtgcgatg ttagctaatg ctttaaaaaa aggtgcatct gaatcgaaag
    19741 caccgatcag tgcaatggcg ggtgcttatg gttctcatta cgaaatgctg caggattttg
    19801 atatggtggt cttagcaccg caagtcaatt cttactatga tgatatcaaa gaagacaccg
    19861 atcgcttagg aatcaagctg gttgcgacaa aaggagcaga atatatcgga ttaacgagag
    19921 atcctgaaaa agctgttcag tttattctga accaatttca ataaaggagg agccataatg
    19981 aaaatgggaa tttggttgcg taaagctgtt ttatttgcag ctgtgttagg ggctttgggg
    20041 gctgtttcaa aaactgttga agcatctgcc agcgaattta ttcgcggagc agatatttcg
    20101 atcctgtcag atatggaaaa atctggtgca aaatattatg aagacgatat ttcaaaagat
    20161 gccttagaga ttttaaaaaa taatggtgtc aactatgttc gactgcgttt gtggcaggat
    20221 ccttataata gtaatggaga aagttatgga gcagggacaa atgatctgga aacgacgatt
    20281 gaccttgcaa aacgagcaaa aaatctggga cttaaggtgc tgctggattt tcactacagt
    20341 gatttttggg ttgatcctgg gaaacagaat cttcctaaag catggcaagg tctgaccttt
    20401 gaagaaatga atacggcatt gtacgactat acaaagaacg ttctgtcgga aatgaagcag
    20461 ctggacgttt atcctgatat ggttcagatc ggcaatgaac tgaatagcgg aatgctctgg
    20521 ccttatggca agtcttgggg agaaggcggg ggagaatttg atcgattggc tgcttttttg
    20581 aagtctggta tccaagctgt tcgagacact caacctaaaa caactccagt aatgctccac
    20641 ttagctgatg gtggcgatac aggagcattt acttggtggt ttgatgaaat cacgagtcga
    20701 ggtgtgtcat ttgatttaat tggagtgtcg tattatccgt attggcacgg ttcaatgaat
    20761 gaacttcaaa ccaatatgaa tgcgatcagc caacggtatg gtaaagaagt gatcgtagtt
    20821 gaaacagctt atgggcatac aacagcgaat gccgatacta tgccaaatgc ttttggtgaa
    20881 gccgaagcag ctgcgggggg atatgaacca acaccgacag gacaagcaga gtatttgctg
    20941 gatcttgccg atcggattca ggcagtaccg aataatcgcg gggcgggctt cttctattgg
    21001 gaacctcttt ggtacaacgg aaatgtgagt tgggcaacac aagcaggtat gaattatctc
    21061 agtgttcaat ccactatggg aaacgaatgg gacaaccaag cgatgtttga ttttgctgga
    21121 aacgccttgc caagcttgcg tgcgtttaaa caagcagggg cgcaaataaa tctagttaaa
    21181 aatgcaagtt tcgaaagcga tggaccaacg accagtcctt ccaattggca aacctggttt
    21241 caacaaggaa ccgctaaggg tacgatcaaa acggaagcgg gcactttttt tggggactac
    21301 aaactaactt tttggcagga tcaagcatac gaggcatcag cttatcaaaa tgttagtggc
    21361 ataagcaaag gaacttacac attgagtgcc tatgtaatgt ctgggggaga gtttgatact
    21421 aatcagttat atatcaaaaa ttatggtgga gcggaactaa atactgcggt tgttaaaaca
    21481 agtgaatgga caaaaattac aatcaaagac attccaatta caacaggtgt ttgtgaaatt
    21541 ggggtttata gcaaggcttc aggaaatgct tggacgaacg ttgattttgt ttcattggtg
    21601 aaacaataga ctgcggatcg tttcgattca agatcggagg ttcccttcag ccacatcgtt
    21661 agcgtagaag gcaggaatcg aacctgcacc tcgcctttgc caaaaataac cagaaacgat
    21721 ttccagtcat ttttaccaaa acgagtaaag caccagactt catctacact ttgtattatg
    21781 ctaaagagag ttcaacgatt tcgtaagaag caatgaaaaa attgcaaaaa gttgtgcaga
    21841 taacataaag tttgtttaag aaagggtaac cttagaattc cgtcgtgaat atgatttgag
    21901 cacttagatg gatagaaaaa agttctcgga aaacagttcg tttttcgaga acttcttttt
    21961 atgataagaa tgaaacaaat taacttatat tatagtctct tttggtaggg aaccatctta
    22021 tttttaatga aaatcaagca ttttagctat tgaagaatgt tgatttatag ggattccccc
    22081 tccatattat gttggctctt tattcccact ccactgttgc tggtggttta gaagtgatgt
    22141 catacacgat acggttgacg tgggctactt cgtttacgat acgtacagag attttttgca
    22201 agacttccca tgggatacgg gcaaagtcag cggtcattcc gtcgattgaa gttacagcac
    22261 ggataccgac tgtgtagtcg taagtacgtc cgtcacccat aacaccgaca gagcggatcc
    22321 ctggaagaac agtgaaatat tgccagatat cacggtcaag tccagcagct gcgatttctt
    22381 cacgcaagat agcatcagat tcgcgtacga tttctagctt gtcttcagtg atttcgccaa
    22441 gaacacgaat accaagacca ggaccaggga atggctgacg ccaaacgatt gaatcaggca
    22501 tgcctaactg agtccctaat tcacggactt cgtctttgaa taatgtgttt aatggttcga
    22561 tcaattcaaa ttgcatatct tctggcagtc cacctacatt atggtgagat ttgatcgtct
    22621 gtgctgtttc tgtaccagat tcgataacgt cagtatacaa cgttccttgt gctaggaaag
    22681 agacgccttc ttcaccagca agttttgttg cttcgtcatc aaaaacatat acgaattcat
    22741 tcccaatgat tttacgtttt tgttctgggt cagatacacc tttcaacttg tctaagaagc
    22801 gatctcttgc attgactttg atgatgttca agccgaattt tccgcctaga ctttccatta
    22861 cttgttccgc ttcgcctttg cgtagcagac cgtggtcaac aaagatagat gtcaattggt
    22921 caccgatcgc tttttgtaaa agaaccccaa cgacactaga atcaacgcca cctgaaagac
    22981 caagcaagac ttttttgtca ccaacttgtt cacggatttt ttctacttgc atatcgataa
    23041 agttatccat tgtccagtta ccttcacagt gacaaacatc aaaggcaaag tggcgcagca
    23101 attcgattcc gtattcggaa tgacgtactt ctgcatggaa ttggatacca taaaaattac
    23161 gtgttgtgtc ttggatagaa gcaatcggac aatctttgct tgttccgact gtttcaaagc
    23221 cttcaggaac tttagttacc aagtcaccat ggctcatcca tactgtttgt tgttttggtg
    23281 tttctttaaa caagacagct tgatcgcttg ttacttctaa caaggctttc ccatattcgc
    23341 ggtttttagc aggctctacg gaaccgccaa gtttgtacgt gatcaattgc ataccatagc
    23401 aaatccctaa tacaggaata cctaaattga agatctcagg atcgatccca aatgcattct
    23461 catcatatac actgtttggt ccgccagaaa gaatgattcc ctttggattc atcgctttta
    23521 cttcttctgc agtaatgcgg tggctcaata attcggaaaa gactccaaga tcacgaatac
    23581 gtcttgtgat caattggtta tactgactac cgtagtctaa tacgataatc ttttcaacgt
    23641 tcgtcatatc ggcaacgttt gtcaaattta ttacccctat ccctttaaat tttatctcaa
    23701 aatagtcaaa ctattttaaa atgctaatat tttcgcatga aaatcgaatc gatgatatga
    23761 ttttcagttt tatggagaat tatatcggct cttcctcgtg taggcaagat atattcctgt
    23821 aaatttttca aattcacatt tttccataca tcttttgcca ttttaaaggc ttcttcacga
    23881 tctccgatag caaaacgata ataataattc tctggatttt ggaaagccgt atccagtaac
    23941 gcaccaaaac gatccaaata ccatttttcg atcagcacgg gatcggcatc gacatagaca
    24001 gaaaaatcaa aaaagtcgct gacgtagatt tgctgattcg caggtaattg aagcgtattg
    24061 atcccctcaa cgatcaagat atcaggctgc tggatcaatt cgtacttgtc tggaatgata
    24121 tcatacacat catgtgagta aacaggtact tttatctctt cctgaccaga ttttacacgg
    24181 ttcaagaaat cgatcagcat ctccatatca taactttcag gaaatccttt acggtccatg
    24241 attccacgct cttttaatat acgatttgga taaagaaatc cgtcagtcgt gatcagctga
    24301 acgtttcttc gtttgaaggt acgagacaaa atcgtctgca gcagtctggc tgttgtactt
    24361 tttccaacgg ctacacttcc ggcgatcccg ataataaaag gcggtacttc tacgtattta
    24421 tgaagaaata aacctttact caagaacaaa gattcaaatt ctttcatata aagataaatc
    24481 agatgagcaa gaggtacata gatatctcga acatcctgca tggaaatgcg gtcattcaaa
    24541 cttttgatat tatctagttc atcttgtgtc aaaggtgctt gcccattacg ataaaattct
    24601 cgccattctt ctcttgccac acggtagtag ttcattggat cattcatatt agcctcctgc
    24661 gttctcccat caatggaact attttaacac aaaataaacg caaaccccac cacttaagag
    24721 agattgccga tctttttgta taatctttat aatagaaaga aacggagtct tattcttgac
    24781 ttgcgcacgg atacataaaa ggatagactg ttgtcatgaa agaaatgaga aggatggaag
    24841 acatgacaaa tcattactat tctgaacgcc cagatacggc gcacgatttt gaacaatggg
    24901 cttttgagtt aaaaggcaga acctttcaat ttgtaacaga ttctggtgta ttttcacgtg
    24961 aaacagtgga ttatggttca cgtgtcctga ttgatgcttt cgattgggaa gagttacctg
    25021 atgaaggaaa gatcttagat gtgggatgcg ggtatggacc gattgggtta gctttggcat
    25081 ttgcgacaca gcggtttgtt gaaatggtcg acattaatga acgagcggta agtctagcgc
    25141 aaggaaacgc aaagcgtaac caaatcgaac atgttgatat ccatcaatcg aatatctatg
    25201 aagcagttca tgaagaaacc tatgcagcta tcgtaagcaa tccgccgatc cgtgctggaa
    25261 aaaaagttgt acatgaaatt ttgacaggcg catatcctcg gttgaagaag ggcggtacac
    25321 tgacaattgt gatccagaag aaacaaggag cgccgagtgc gcaaaagaaa atggaagaaa
    25381 catttggtaa tgcagaaatc gtcacaaaag ataaaggata ttatatcatc aaaagtgtga
    25441 aagaataact agctgaaaaa tagtggagcg acgaaagagt ggaactgggc gtgcatgtac
    25501 gggtacgtat cagttctttt tttgttgaga agtttttaaa aacaaagaac tattttttct
    25561 tcaaaaagaa atagtaggaa cagagtcgtt gttttttacg ataaaataaa taagatacag
    25621 aacttgtata gaaggctgcc aagtcggtag ataaaggagg tttttatcaa aaaacatact
    25681 ttcctttttt ctctattctt ttcttggggg ttttggtaca ctgataggga atttagatag
    25741 aagggagcca ataggatgaa gctgaccatc agagatatcg cagaaatggc aggcgtatca
    25801 gtaacaactg tctcacagat tttgaataac aaaggaagca gatttagtga taagacaaga
    25861 aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc ccgattattt cgcttcaaat
    25921 gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc ctgatgtcac tgatttcttc
    25981 ttctccaaac tgatcgaagg tgtagagagc tatttgaatc ctttgggcta tgtgatcatg
    26041 ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt atttacaaga gctttcccac
    26101 cgctcagtgg acggaatctt gctggccaca ccaaatattt tacctgagaa atatgcatta
    26161 gacagcggtt tttatcaaaa aatgccaatc attttaatcg atcgcggatt gaatcgtcgg
    26221 gataacggac ggcttatcgt aaaggaatat gagggcgcct atcaagctgt ttcgctgttt
    26281 attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa ccactggata ctatcaatta
    26341 gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc atggcctgcc ttttaatggg
    26401 aaatttgttg aacatggaga gttgactgtc caaggtggat atactgcatc aaaaaaatta
    26461 ctcaaacata aagatatcac ggctattttt tgcgggaatg atgccatggc gatcggatgc
    26521 tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag atatctcagt cataggattc
    26581 gatggattaa agctgtcaga atacatgatt ccgcaattga cgacagtgca gcagccaagt
    26641 tttgatattg gattttatgc tgcacgtttc ctgatcgata cgatcgaatt tcctcaaaga
    26701 aaagttccga acaaagtgtt cgaaacaaaa ttaattattc gtgagagcgt aaaagtattg
    26761 acaaagggct aactcggcgg tatattgctt atgtaagcat ttacaaaggc tagttagtgc
    26821 tttttttatt tttagtaaac cagtttacta aagtggttta cctttcctaa aaataatgta
    26881 taataccgaa tgaagacaag gaggatcagg caatgcgaat ggtggatctg attgaaaaaa
    26941 agagggatgg aaacgagctg tcgaaagagg aaatcgagta catcgtcacg aactatacga
    27001 atggaaagat tccagattat caagtcagtg cattattgat ggcaattttt tatcaagaca
    27061 tgacaaatga agaaatcacg aacttaacat tggcgattgc taattctggt gatgtcatcg
    27121 atttgagttc tcttgaagga ataaaggtag ataagcattc gacaggtggc gttggtgaca
    27181 cgaccacatt gattttagcc cccctagtag ctagtgtcgg agtgacagtg gctaaaatgt
    27241 ctggacgcgg tctaggttac acaggtggta cactagacaa gctagaagca attcccggat
    27301 ttcagatcga gctttcggat gaagaatttg tacgcattgt taatgaaagt aaagtagcgg
    27361 taattggtca atcaggaaac ttggcaccgg cggacaaaaa attgtatgcc ttgcgtgacg
    27421 tcacagcgac cgttgattct cttccgctga ttgccagttc gattatgagt aaaaaaatcg
    27481 cggcaggagc agatgcaatc gttttggatg tgaccactgg agacggtgct ttcatgaaga
    27541 acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag cattgggaaa ctggctaacc
    27601 gagaaacagt ggcggttatc tcagatatgt cagaacctct tggcgaagct atcggaaata
    27661 gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa tggtccagaa gatctagtag
    27721 aaatgtgcta cgccttagga agccaaatgg ttgttcttgc tggcaaagct aagacaaccg
    27781 atgaagcaag aacgttgctt caagaagctc tggaatcagg taaagcgtta gcaaaattca
    27841 aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt ggaacatcca gaacgaattt
    27901 tgactgcacg ctacacaatg gagcttcctg ctaaacaaag cggagttgtg tcaaaaatcg
    27961 tggcaaatga attaggaatt gctgcaatga tgctaggtgc tgggcgtaaa accaaagaag
    28021 aagatattga tcacgctgtt ggattgaaac tgcataaaaa aattggtgac actgtgacaa
    28081 aaggtgagtc actattgacg atctatagta acgacgaaga aatcacttct gtcattgaat
    28141 tattatataa aaatattgaa attggtgaat cggcaatgaa gccaacgttg attcatgaca
    28201 tcatcacaga ataaaggaaa aaagcggact ttaccgtttt aaagaaaagg ggaaaagaaa
    28261 tggaactaaa ccgaatgatc gaccatacta ttttgaaagc agacgcatct aaagaagctg
    28321 tgatgcaaat tatcgaagaa gcaaaaaaat accattttta ttctgtctgc atcaatccag
    28381 catgggttgc gctagcgaaa gaacaattac aaggtgagcc agtagccgta tgtacagtaa
    28441 tcgggtttcc tctaggagca aacacacccg agacaaaagc atttgaaaca acagatgcca
    28501 tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat cggtgctttg aaatcaaagg
    28561 atgatcaaca agtccaaaaa gatatcgaag cagttgttga agcagcaaaa gatcgtgcgc
    28621 ttgtcaaagt tattattgaa acatcgttat tagatagaga agaaatcatc cgagcttgcg
    28681 aaatagcaaa agctgcaggc gctgattttg tcaaaacatc aactggtttc tcaacaggcg
    28741 gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt aggaccagaa atgggtgtca
    28801 aagcttccgg cggtatccat aacgaagaag aagcaatggc aatgatcgaa gctggtgcta
    28861 caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg ttcaactggt gaaggctatt
    28921 aattttttca aaggagaagc tcatggcaga actcaaacaa gaatggatca acacagctat
    28981 tgaagcgtta gataaagcct atgttcctta ttcccatttt ccggtaggcg cttgtctcgt
    29041 aacggaatca ggcaagattt atcaaggcat caacattgag aatgcatcat ttggattaac
    29101 gaattgtgcc gagcgcacag cttttttcaa agcagtatct gaaggagaaa aaacatttac
    29161 gcatctagtc gtagcggggc acacccccga tcctatctct ccttgtggtg catgtcgaca
    29221 agtaatggca gaattctgtt cccctgatat gccggttacg ctagtaggag acaatggtgt
    29281 gacgaaggca acgaccgttc gtgcactgct gccttatgca tttactgaaa aagatctata
    29341 actcatgttc attcagtcag ctgactgttg aaaaataaaa caattatttt aggaggcttt
    29401 acagaaatga aaaaagcaaa attatttggt ttaggtgctg tcgcactagc agcagggtta
    29461 tttttaggtg catgcggcaa caacggctct actgattcat ccggtggcaa aagctcatca
    29521 gacacaacaa ctgcagcatt gatcacagac acaggtggtg tagacgaccg ttcattcaac
    29581 cagtcagctt gggaaggact agaaaaatgg gggaaagatc aaggactttc acgaggaaat
    29641 gatggcttcc aatatttcca atcttcaaat gaatcagatt acatcccaaa tattgaccaa
    29701 gcattgaatg ctggattcaa aacgattttt ggtattggtt ataaactaaa accagcgatc
    29761 caagaacaag caacaaacaa tacaggtacg aactttgtta ttatcgatga tgtcatcgat
    29821 ggattagata acgttgtatc tgcaactttc aaagacaacg aagcttctta tcttgcaggt
    29881 gttgcagcag cttatacgac tgaaacaaac gtagtcggct ttatcggcgg tgtaaaaggt
    29941 gaagtaatcg atcgttttga tgctggattc aaagccggtg ttgatgcagg tgcgaaagaa
    30001 ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg acttctctgc tccagataaa
    30061 ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag atatcatctt ccatgcttca
    30121 ggcggtacag gtaacggtgt attccaagaa gcaaaatcat tgaacgaatc aggcgacaaa
    30181 aaagtttggg ttatcggtgt tgaccgcgac caatcagacg aaggcgaata cacattaaac
    30241 ggcgaaaaga aaaacttcac attgacatct acactgaaag cagttggaac agtagtagaa
    30301 gatttggcac aaaaatcagc ggacggtaaa ttccctggcg gcgaacacac tgtttatgga
    30361 ttgaaagaag acggtgttgg tctaacagaa ggacaacttt ctgacgaagc gaaaaaagca
    30421 gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa aagttccaga aactcctgaa
    30481 gaaaactaaa accatttcta tagccttgtt agacacatgt ttgacaaggc tatggtttga
    30541 ttagaacaag aattgttcat ttatccaagc tgccttgtga cagtttggat aagggaacaa
    30601 ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac taagcgtgaa tcttagtcaa
    30661 tcgttgccct agatagaaat cgttaggatg tgaaaaaaag gtggttcaag aaaatattgt
    30721 catagaaatg cagaatatta ccaaagaatt tggaacattc aaggcaaatg acaacatcaa
    30781 tttacaagta aaggcaggag aaattcatgc gttgcttgga gaaaacggtg ctggcaaatc
    30841 tacattgatg aacgtgcttt ccggattatt agagccgaca tcagggaaaa ttttgatgcg
    30901 tgggaaagaa gtaaagatca caagtccgac caaagccaac caattaggga ttgggatggt
    30961 ccatcagcac tttatgcttg ttgatgcctt tactgtaaca gaaaacatcg tgttgggaag
    31021 cgagcctagt cgcgcaggga tgcttgacca taaaaaagca cgaaaagaga tccaaaaagt
    31081 ttctgaacag tatggattgt cagtcaaccc agatgcttac gttcgtgata tttcagttgg
    31141 aatggaacaa cgggtagaaa ttttaaaaac actttaccga ggagcagatg tactgatttt
    31201 tgatgagccg acagctgtat tgacccctca agaaattgat gaattaatcg tgatcatgaa
    31261 ggaattagtc aaagaaggca agtcgatcat tttgattacg cataagttag atgaaatcaa
    31321 agcagtagct gaccgctgta cagttatccg ccgtggaaaa ggaatcggta cagtcaacgt
    31381 taaagacgta acctcacaac aattagccga tatgatggtt ggaagaacgg tttcattcaa
    31441 aacgatgaaa aaagaagcga agcctcaaga agtcgttttg tctattgaaa atctagtggt
    31501 gaaagaaaat cgtggattag aagccgtgaa aaacttgaac ttagaggttc gtgctggcga
    31561 agtacttggt atcgctggaa tcgatggaaa cgggcagtcg gagttgatcc aagctttgac
    31621 tggtttgcga aaggcagaaa gcggacatat caagctaaaa ggggaagaca tcactaataa
    31681 aaaaccccga aaaatcactg aacatggtgt aggacacgtg ccagaagacc gtcataaata
    31741 cgggttagtc ctagatatga cattatctga aaatatcgca ctacaaacct atcatcaaaa
    31801 accttacagt aaaaacggca tgctgaatta ttcagtaatg aatgaacatg ccagagaatt
    31861 gatcgaagaa tatgatgttc gaacaacgaa tgaacttgtt cccgcaaaag ctttatcagg
    31921 cggaaatcag caaaaagcaa ttatcgctcg tgaagtcgac cgagatcctg atctgttgat
    31981 tgttgcaaat ccaactcgtg gactggatgt aggtgcgatc gaatttattc ataaacgtct
    32041 gatcgaacaa agggacaaat acaaagcagt gttactgatc agtttcgaat tagaagaaat
    32101 tttaaatgtt tcggatcgta ttgctgttat ccatgaagga gaaattgtcg gtatcgttga
    32161 tccgaaagaa acatctgaaa atgaattagg tctattgatg gcgggctata ccttagaaga
    32221 agcacgaaaa gagttagaaa agggggctgg cgcacatgaa tgatcgcagc gaaaaattcc
    32281 gtaatttatt agtgcctgta ctttcagttg ttttaggatt gatcttaggc gcgatattga
    32341 tggctgcttt tggttttcag cccatccaag gttattcttc tatgctagat gcagcccttg
    32401 gttcgcaaag agggataggt gaaacgttaa ggcaagcaac gccgttgatt tttacagcat
    32461 taggtttttc tgtagctaac tcagctgggt ttttcaatat tgggctttct ggacaagcac
    32521 tttgcgggtg gatcgtcagt gtctgggtgg cacttgcgtt tccagatctg ccaaaagttg
    32581 ttttgcttcc gttatgcgtg atcttaggtg ccttagctgg tgcgatggca gcggctgttc
    32641 caggtttact tcgagcattt tttggaacaa gtgaagtgat cgtaacaatc atgatgaact
    32701 atatcttatt atacgtaagc acatttgctt tacatgaagt gatgcctgaa agctatcgct
    32761 caagtttgga ctcttccaat atgatcagtc aaaatgcctc actgcggtta ccatggttaa
    32821 cacaagcttt tggcggctct cgcgtaaact caggattatt tttagcaatc atcgcgctta
    32881 ttttcgtttg gctgctgatg aagaaaacga ctttaggatt tgaaatccgt tctgttggat
    32941 tgaatccttt tgcttctgag tatgcaggaa tgagtagtaa acgtacgatc gttttgtcga
    33001 tggtcatttc tggcgctttg gcagggcttg gcggtgtggt tgaaggatta ggaacgtatc
    33061 aaaacttctt tgttcaaacg acttcgctat ctattggatt tgacggaatg gctgtttcct
    33121 tgcttggttc tggatcagca atcggtattc ttttatcagc gatcttgttc agtattttga
    33181 agatcggtgg acttggcatg cagacaggag caggtgttcc gtttgagatc gtcaatgtat
    33241 cgattgcttt gattatcttc tttgtcggga tcaacttcct gattcgtttc gtcatggcta
    33301 aagtcttcca agggaaaaaa caagaagaga tcgttgcgac aatagaatcg aaaccaacga
    33361 atcaaaacca tgaagggggc gaactataat gtcgactatc gaattaattg ccttgatcct
    33421 tacttctaca ctggtttatt caacaccgct aattctaacg tcactaggcg gaactttttc
    33481 tgaacgaagc gggatcgtaa acgtagggct agagggaatc atggtcatgg gtgcttttag
    33541 ttccatcgtt ttcaacttga cgatgagtcc atcacttggt tctatgacac catggatcgc
    33601 tattttagtg ggtggcgtcg taggtatcct attttctctg attcacgcag tagcaacagt
    33661 cagtttgcgt gctgaccata ttatcagcgg gacagtaatt aatctgatgg cacctgcttt
    33721 aggtgttttc ttgatcaaag catggtatgg taaaggtcaa actgataaca tcacgcagaa
    33781 tctggggtat ttttctttcc caggattagc agatattccg gtcattggac caattttctt
    33841 taaaaataca ttcttacctg cttattgtgc tattttgatt gctgtcctag cttgggtgat
    33901 catcttcaag acgaaattcg gcttgcgcct ccgttcagtt ggtgaaaatc ctcaagctgc
    33961 cgacacatta ggtatcaatg tatatgctat gcgttattca ggcgtactga tttctggctt
    34021 gttaggcggt atgggcggtg ctgtttttgc tcagtcgatt tccggtaact tttcagctgg
    34081 tacgatcgtt ggacaagggt tcatctccat ggctgctatg atctttggta agtggaatcc
    34141 tttaggggca atgggtgcct cattgttctt tggttttgct caaagcttga gtatcattgg
    34201 cgcacaatta ccagttattt cttcgattcc gccagtattg ctgcaaattg ctccatattt
    34261 gctgacaatc attgtgctgg ttgttttctt agggaaagct tctggtccaa aagcaaacgg
    34321 gaagaactat atcaaatcaa aataatcttt tagcgaataa aaagggtggg ttacgggaag
    34381 tgtttttcgt ctcgcccttt ttgttttaaa ggcaataaaa ataaaaagaa agaaggaata
    34441 atcatgttta aacgcgtaca tctgatcgtc atggattcag taggaattgg agaagcacca
    34501 gatgccgata agttcggtga tgttggttct gatacattag gacacattgc gaaagaagca
    34561 ggattgaacg ttccacattt agagcagctt ggtttaggaa cgatccgtcc tcttgaaggt
    34621 gtggaatcag tgaaagatca tcaaggctat gcgacgaaat tagaagaagt ttctgtaggg
    34681 aaagatacga tgacaggcca ctgggaaatc atgggattga atatcaaaac tccatttcgt
    34741 gtgtttccta atggattccc tgatgatctg atcaaacaaa tcgaagaatt ttctggccgt
    34801 aaagtcgtat gtaacaaacc ttatagcgga acagctgtta ttgacgatta cggtgaacac
    34861 caaatgaaaa caggcgattt aattgtatat acttctgccg atcctgtcct tcagatcgcg
    34921 gcacatgaag aaatcattcc tttagaagaa ttatatcgga tttgccaatt cgttcgtgat
    34981 ataacaaaag atgatccgta tatgattggc cgaatcattg cacgtccgta cgtgggtgaa
    35041 ccaggagact tcacacgaac aagcaatcgc catgattatg cgttgaatcc attcggacac
    35101 acagtccttg attctttgaa agaagcagga aaggacgtga ttgctgttgg aaagatcaac
    35161 gatattttca acggacaagg aatcactgaa tctgtccgca cgaaaagcaa tatggatggc
    35221 gttgaccagc tgctgaaagt tatgaaaaac gatttttcag gaatcagctt taccaattta
    35281 gtagattttg atgcattata cggtcatcgt cgggatgtag tcggctatgc gcatgcgatt
    35341 gaagaatttg acttacgaat tccagaattg cttgaagcga tggaagaaga cgacttgcta
    35401 atgattacag ccgatcacgg aaacgatccg acgtttactg gtaccgatca tacgcgagaa
    35461 tatgtaccgt tattggttta cagtaaaaaa atgaaagaac aaggggcatt gcctcaagga
    35521 tattattcag atatttcagc aacgattgct gagaacttta gtgtttcttc gactgaaaat
    35581 ggcgaaagct tcttgaaatt attaaaataa tctttttaaa taggagaaag caaatgacca
    35641 agttaagcga gatgttacaa gaaacgaccc agtttatcct tgataaagga gtaaaagaaa
    35701 ttgattttgg tttgattcta ggttcaggat taggtgaatt agcagatgaa atcgaagatg
    35761 ctgtaatgat ttcttatgat gagatcccgt tctttcctgt ttcgacagta gttgggcatg
    35821 caggaaagct agtttatggg tctctggcag gcaagaaagt gttagccatg caagggagat
    35881 tccactacta tgaaggtcat tccatgcaaa cagtgaccta tccagtacgt gtgatggccg
    35941 gactaggggc ccattcagtc attgtgacta atgcatgtgg tggtgtcaac gaatcatttg
    36001 taccaggaga tttgatgctg attaccgatc atatcaattt tactggtgaa aatcctttga
    36061 tcggtccgaa tgaagatgca atgggcccta gatttccaga tatgagtcaa gcttatacgc
    36121 ctgactatca agcaacagca aaagcagcag cagaaaaact aggtttggat cttaaagaag
    36181 gcgtatatat gggctattct ggaccaactt atgaaacgcc tgcggagatc cgaatgtctc
    36241 ggacaatagg agccgatgca gtagggatgt cgactgttcc agaagtcatc gtagcagctc
    36301 acagcggatt aaaagtgtta ggaatctctt gtattaccaa cttagcagca ggtatgcaag
    36361 cgaatctgag ccatgaagaa gttgtagaaa caactcagag agtcaaacaa tcttttaaag
    36421 cattggttaa agaaacattg acattattgt aataggagga acaataaaat gagtgtgcat
    36481 attgaagcga aagaaggcga gatcgccgat aagattttac ttccgggaga tcctttgcga
    36541 gctaaatata ttgcggagac ttttttggaa gatccagttt gttacaatca ggtacgcggt
    36601 atgttaggct ttaccggcaa atataaagga gaacgtattt ctgttcaagg aacaggaatg
    36661 ggaatgccat cagccaccat ctatgcccat gaattgatcc agtcgtatgg tgtaaagaaa
    36721 ttgattcgag taggtacatg cggtgcattg tcaaaagacg tccatgttcg tgatttagta
    36781 ctagcacaag gtgcagcgac cagttcttcg atgatcgaaa aaaatttcca agcctttcat
    36841 tttcctccaa tcagtgattt caatctatta ctgaaagcat atgagatagc taaagaaaag
    36901 ggctatacgg ttcatgtagg aaatgttctt tcggaagatt ctttctacaa agatgatttg
    36961 acagagacgt tccaattagc tgaacttggc gttttaggag tggaaatgga agcagcggct
    37021 ctttattact taggcgcgaa ataccatgtg cagacattaa gtttgatgac cgttagcgac
    37081 catttgatca ccggtgaaga aacaacagct gcagaacgtc aatctacgtt caatgaaatg
    37141 atcgaagtag gattggaaac agcgatagca gaataagaag gaaatgaaaa agctgccctg
    37201 cattaagtaa taagaacatc caagcaaaaa tagctcctca tgtttttgac ttggctggac
    37261 ttattattgc aaatgtaagc ttttgaacac cgtttaccaa gaggttgtga a
//
LOCUS       DACEDM010000022        37293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEDM010000022 DACEDM010000000
VERSION     DACEDM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.022"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            84..830
                     /gene="truA"
                     /locus_tag="IWO50_001002"
     CDS             84..830
                     /gene="truA"
                     /locus_tag="IWO50_001002"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0358139.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(1285..3954)
                     /locus_tag="IWO50_001003"
     CDS             complement(1285..3954)
                     /locus_tag="IWO50_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0358140.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(4192..4380)
                     /locus_tag="IWO50_001004"
     CDS             complement(4192..4380)
                     /locus_tag="IWO50_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0358141.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            4650..5012
                     /locus_tag="IWO50_001005"
     CDS             4650..5012
                     /locus_tag="IWO50_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0358142.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5064..6746
                     /locus_tag="IWO50_001006"
     CDS             5064..6746
                     /locus_tag="IWO50_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0358143.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            6863..8899
                     /gene="recG"
                     /locus_tag="IWO50_001007"
     CDS             6863..8899
                     /gene="recG"
                     /locus_tag="IWO50_001007"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0358144.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            8948..9949
                     /gene="plsX"
                     /locus_tag="IWO50_001008"
     CDS             8948..9949
                     /gene="plsX"
                     /locus_tag="IWO50_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0358145.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            10071..10313
                     /gene="acpP"
                     /locus_tag="IWO50_001009"
     CDS             10071..10313
                     /gene="acpP"
                     /locus_tag="IWO50_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0358146.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            10581..11585
                     /locus_tag="IWO50_001010"
     CDS             10581..11585
                     /locus_tag="IWO50_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358147.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            11586..12530
                     /locus_tag="IWO50_001011"
     CDS             11586..12530
                     /locus_tag="IWO50_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358148.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            12530..13492
                     /locus_tag="IWO50_001012"
     CDS             12530..13492
                     /locus_tag="IWO50_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358149.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            13519..14433
                     /locus_tag="IWO50_001013"
     CDS             13519..14433
                     /locus_tag="IWO50_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358150.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            14459..16240
                     /locus_tag="IWO50_001014"
     CDS             14459..16240
                     /locus_tag="IWO50_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0358151.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            16588..17274
                     /locus_tag="IWO50_001015"
     CDS             16588..17274
                     /locus_tag="IWO50_001015"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0358152.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            17294..20875
                     /gene="smc"
                     /locus_tag="IWO50_001016"
     CDS             17294..20875
                     /gene="smc"
                     /locus_tag="IWO50_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0358153.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            20884..21693
                     /locus_tag="IWO50_001017"
     CDS             20884..21693
                     /locus_tag="IWO50_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0358154.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            21704..22702
                     /gene="ftsY"
                     /locus_tag="IWO50_001018"
     CDS             21704..22702
                     /gene="ftsY"
                     /locus_tag="IWO50_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0358155.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            22889..25156
                     /gene="gshAB"
                     /locus_tag="IWO50_001019"
     CDS             22889..25156
                     /gene="gshAB"
                     /locus_tag="IWO50_001019"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0358156.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(25245..25697)
                     /locus_tag="IWO50_001020"
     CDS             complement(25245..25697)
                     /locus_tag="IWO50_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0358157.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            25847..26797
                     /locus_tag="IWO50_001021"
     CDS             25847..26797
                     /locus_tag="IWO50_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0358158.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            26790..27749
                     /locus_tag="IWO50_001022"
     CDS             26790..27749
                     /locus_tag="IWO50_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0358159.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            27746..28501
                     /locus_tag="IWO50_001023"
     CDS             27746..28501
                     /locus_tag="IWO50_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0358160.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            28524..29477
                     /locus_tag="IWO50_001024"
     CDS             28524..29477
                     /locus_tag="IWO50_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0358161.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(30000..30296)
                     /locus_tag="IWO50_001025"
     CDS             complement(30000..30296)
                     /locus_tag="IWO50_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0358162.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            30651..31007
                     /locus_tag="IWO50_001026"
     CDS             30651..31007
                     /locus_tag="IWO50_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0358163.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            31017..31916
                     /locus_tag="IWO50_001027"
     CDS             31017..31916
                     /locus_tag="IWO50_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0358164.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            32433..33164
                     /locus_tag="IWO50_001028"
     CDS             32433..33164
                     /locus_tag="IWO50_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0358165.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            33309..35336
                     /locus_tag="IWO50_001029"
     CDS             33309..35336
                     /locus_tag="IWO50_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0358166.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            35444..36541
                     /locus_tag="IWO50_001030"
     CDS             35444..36541
                     /locus_tag="IWO50_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0358167.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            36848..>37293
                     /locus_tag="IWO50_001031"
     CDS             36848..>37293
                     /locus_tag="IWO50_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0358168.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        12484 a         6508 c         7920 g        10381 t
ORIGIN      
        1 tttatctgaa aagaaagaac gaaaaaattt tatagaaaaa tcattattcc agataacctt
       61 ttaggaacaa aggagagaaa aacatggtac gttacaaagc aatcattgcg tacgacggaa
      121 caaattttaa tggattccaa aaacagccaa atgggcgtac tgttcaagaa gaagtagaaa
      181 aaacattaca aaaaatggca aatggaaaag aaatcacggt ctttggttcg ggaaggacag
      241 atgccggagt acatgcgatc gggcaagtaa tccattttga ttatccagag gagcgtccgt
      301 tagaacgaat gcgtttcgcg ctagataccc agtcaccaga ggatattgct gtaagacaag
      361 tagaaattgt ttcagaagac ttccacgcaa gatatttggt caaagaaaag acgtatcaat
      421 ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg ccattatgca agttattttc
      481 cttacccact tgatttggaa aaaattcagc gggcgcttcc tgatttatta gggactcacg
      541 atttcacctc tttttgtgca tcaggaagct cgatagagga taaaatacgt acgatctacg
      601 aggctaagat ggaggtaaat gaaacaaaag acgaattgtt gtttacattt aggggaaatg
      661 gcttcttgta taagatgata cggatcttag taggaacgct tttgaaaatt ggcaatggcc
      721 gattgcctga agatagtatc ccagatatta ttgcgaaaaa agatcgtaat gcagcaggac
      781 ctacagcaca ccctgaagga ctttatttgt atgaagtgaa atatgaataa gaggttatga
      841 tagaagtggt cagcttcaag aaataagaag gtatttacga aaattacttc tcaaaatttt
      901 gcaaaatttc ggcttatttt cgaagaagct acttctggaa caccgtttaa ggaagaggtc
      961 gtgaaatcag cgttttgacc tgagtattag agaaacaaac gggaaaatag ctcctcatat
     1021 tttgccgttt gttgagctaa taccgaaggt cagctgattg aacaccgttt aagaaagagg
     1081 tcgtgagcct gtcatttaac tctgagtcac aaagagcaat ttcaaaaaac agcttctcat
     1141 ctttgatgaa tatcgtgact tgtggacgag gcgttgataa gtgaatcatc tttttgttac
     1201 ctattttttc tgtaaccaaa aaacgatgga acgttgtttt gattgccctt ggtgcggaca
     1261 gtctgaacag gttccatcgt ttctttattt tctacgtctt cttctgtgtg tgtttcgtgg
     1321 ttgttcttct attgctggat caatcgtgcc ataaaagggg cgattgcggt caaggtacca
     1381 gacataaaga ataaacaatg caatacatag gacaaaaagg attgcaccta tcgtaaatcc
     1441 ttcaggcaag taactgaaag tgacagtatg tttacctgct ggaactttta tcatcaagaa
     1501 tgcgtctttg aaattgctga ccttgactgg tttcccatca attttagctt tccatccttt
     1561 gtcataagga atagttgtca ccaacatgga atcttcagcg gtatttacag tcccagaagc
     1621 ttttctgcca gtggcttcca tttcgacacc tttttctttg atctgattca ttgcggcttg
     1681 ataggctaca acatctagac caacgacttt aggctccatg aaacttattt ctttcgtccc
     1741 ataaaaactt gcagtaaatg tcactgttgt ctcttgagga taatagccaa gatcatagta
     1801 ttgaccagaa atgttgattt gcgtttttcg cgatgtacca ttgacagtga tcgtcgcact
     1861 agaactttct aattgcgcaa aattggttgg gaacaaactt aagtaagctt gtgtatttgc
     1921 aggcacatgg acttcccaag tgagctcttt tggctggtta tctgctgttt ctgagtaagt
     1981 gacgccagtc gttgtctttg tgatcgtcgt attcaactga ttcttcaaag taggtgtata
     2041 gaatgtaaaa tactgttcat ttagaccaga caatgtattg atcaagtttg tctgactggt
     2101 caaattatct gtcactggct gctcaacatc attaacggac aacggtgcag taaatgctag
     2161 tggcaatgca ttgacattct gatacagtgt atagtcgccg ctttgaccag cttggcggaa
     2221 gccatattta ctgaacgtgc tttcgttttt agaaatgtta tatttgattc ctgtaaaccc
     2281 atccatcagc aaagtattat tgttataacg gatattcaaa tttgtccctc ttgaacggaa
     2341 acctaattta tccaggtaag aggaagaatt acgatttctt atagaagaaa acaaactgat
     2401 accactgtaa ccatagttga tcgaatcgtt tgcagatact ggatccagat tttccaagcg
     2461 gtaaaacgag tcggaatctt tttttgtttc atttacaaga gctttcaagt caggataagg
     2521 atcagtatat aaactgcggg aagcataatt ccaatcatca agaatgccat ggatcatact
     2581 gttcgtattg accagtgctt cagcagacat gatcaataat aacaatatac tcagatgatt
     2641 gaatgaaagt tttttatgct gataaaatcc cacagcaaaa gcataaatcc ctaagaaaac
     2701 cactgtcaat acaaaggaag tcatcgtcac ataggtgtag cttccttcag gtttgatgcc
     2761 ccatgccacc gcaaagagag cagctacgac caaaatgatt cctaacaaca gcccacgatc
     2821 gtcttgttct atcttttccc atccttttcc tgcaagcagg agcactaaga aagagaacag
     2881 ataggcataa cggaataaaa acatattcgg tgcatgcata ccatgccaga acaaattcaa
     2941 tggaacaaga taaaaacttg cgataagtat ggcgaaaaga ctgccgtaca aaaatttatt
     3001 tttcaacggt actttccgac tggcaaaata aaacagacag aggactaatg gtagcagacc
     3061 tgcgtaaacg aacggaatcg atccgtattt tgtcgtgtca taaacaccaa tcatattttt
     3121 catgattaga tccagataag aagtcgcttc tgttttcaac gtagtgatct gactcagtgc
     3181 ctcgccattt gaccgaagat ccagcactgc tggcaataca atgatcattg aagcgccgcc
     3241 tgccagtaaa gaagtgatac cataaggaac aatcgttttt ttatattctg accagtttgt
     3301 caatagacgg gcgataaaat ataagaaaga aaatacgcca atcataaaac ccatataaaa
     3361 actggataaa aacaagagta aataactgat aaataaaagt ttcggccgtt taagatccat
     3421 aacacgatga atccccaaaa tcactaatgg aagataaaaa aatgcatcca gccacatgat
     3481 gatctccgaa tgtgcagtga taaatgagat caatgcgtaa ctgacgctaa gtgcaacatg
     3541 cccccagcga ggaatcttga acgtacgtgc aagaaaccaa aaagccaatc cggcacagcc
     3601 gaccttaagc aaagtcagaa catacagtgc atctggcatc atctggtttg gaaaaaagaa
     3661 caccagtggc gtgaagatcc cgcctagata gtaagaaatt aaagataaat aattcaatcc
     3721 tagagaagca ttccatgtat aaaaaatact ttgctttcca agaagaacat ttcggaagct
     3781 ggcatgaaaa ttagaaaact gtgaaaaggc atcacttgcc aaaatacttc tgctgcttcc
     3841 aggatagatt ccgatactta gataaacaag tgctaaaatc aaaaacggaa tcaggaaact
     3901 agctgctgcg tagcgtccgt tttctctaaa gaatgacttt aaattttttt tcatatttat
     3961 tttccaccaa atttctatga agttgtattc agtttatcat atccttgttt ttctctgctt
     4021 aaaaagagtc tagttttcac aaaaaaataa aatttagaat aaataacttc ataatttgtt
     4081 cgtataacaa aaataaacgc aaaaaaaggg taggaatcaa aagtcccacc cttaaaaatt
     4141 tttattaaat aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg
     4201 ttcaactttt cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc
     4261 gaccataacg cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc
     4321 gtgtgaacgg ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat
     4381 ggtaatttcc tcctttgcct tgatttggag caatagaata attcagctca taccagacta
     4441 acatatcata tagaatggga atttgcaagt acattttcat agatacaatt gacaattgag
     4501 aagaataatt gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt
     4561 tgggtaactg ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg
     4621 agttttttac ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa
     4681 ccattgaaat taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt
     4741 tcggtgttgt aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc
     4801 gtaaagaaaa ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag
     4861 atgtttatac gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag
     4921 aaaaagtgaa atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct
     4981 ttgtacaagg tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt
     5041 cgatctgaag gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg
     5101 aaatggtcca ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa
     5161 atgtattccc tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg
     5221 gagctaaggc agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt
     5281 caaaaggatt gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc
     5341 gtggattttc taagcaaatt ccagatgttg tctcattaaa tgcttctgat cttgctgcag
     5401 ccttcactca tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga
     5461 ttttgacagt agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg
     5521 atgtcgttga agtaatgaca gctgttgtaa aaggagcaaa gcgtgcttta gctaaaactc
     5581 cagacttgct tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc
     5641 tgtttgttta tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat
     5701 atgaaccttc tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc
     5761 aaggtcaaat ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa
     5821 tcggagaagg acctacagtc gatagtacat tcgattacga agaattccgt aactatttag
     5881 atggtatagg agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc
     5941 atacagaaaa cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa
     6001 tcaaagtcga caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga
     6061 acgagtttgc gaaaaaagaa ataccacgta tcccgtatgg tatcgtagcg atcgctgctg
     6121 gtaaaggcgt ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtggac
     6181 aaacaatgaa tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata
     6241 aagtgattgt cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag
     6301 tagcagatat cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta
     6361 tgctttcatt caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt
     6421 tgtctagcgt tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg
     6481 tagagatcaa agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc
     6541 cagatatcac agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga
     6601 tcgtaacgat cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga
     6661 aactaacaga aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct
     6721 atccttatct attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt
     6781 cattcataaa aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg
     6841 ttaaaggagg gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg
     6901 agtcggagaa aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt
     6961 actgacttat tatccttttc gctacgaaga tattcaggaa agaaatctaa acgagatcca
     7021 ggatcaagaa aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt
     7081 tggttataaa aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt
     7141 ttcttttttt aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt
     7201 atacggtaaa tgggatgcaa agcgtaaagc cttgaacggg atgaagattt taggttctca
     7261 aacaatggat gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt
     7321 agttgatcta atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc
     7381 aaacaacctt gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca
     7441 ttttccaaaa gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt
     7501 ctttttattt caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg
     7561 tttggagatc ctttatgatg tccaaaaatt gaaacaattt actcaaaagc taccttttga
     7621 actgacaaat gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca
     7681 gcatatgcag cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat
     7741 tgcgttatac gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat
     7801 cttggcacaa caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat
     7861 cgcattgatt acggggtcaa cgaaagcaaa agaacgacgt gagctccttg aaaaactaga
     7921 gcaaggggaa attaacgtcg tgatcggcac acatgcattg atccaagatg gagtcgaatt
     7981 tcagaattta ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa
     8041 agtacttcgt gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc
     8101 tagaacatta gctattacag catatggtga gatggatgta tctatcattg atgaacttcc
     8161 agctggacgt atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact
     8221 ggattggacg aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat
     8281 tgaagaatca gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga
     8341 actatttgca ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa
     8401 agaagtaatc atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt
     8461 aattgaagta ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg
     8521 atttggattg gcacagctgc accaacttcg tggacgtgtt ggacgcggaa gtgatgcttc
     8581 ctactgtatc ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat
     8641 gacagagacc accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg
     8701 tgaagtattt ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac
     8761 ggatggaaat gttttggagg tagccagaga agaggctgca gccctttggc aaataaagga
     8821 ttggcagatg cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga
     8881 taatcgttat ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt
     8941 ggaaacaatg aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt
     9001 agaaggcgtg gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa
     9061 agaagcagaa atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga
     9121 cgaaaaaatc aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc
     9181 aatggtcctt gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg
     9241 gaatacaggc gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga
     9301 acgccctggc ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat
     9361 gctggatctt ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt
     9421 aggttctttt tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa
     9481 caacggaaca gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca
     9541 gaatgaaaca agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt
     9601 ggctgatgta gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg
     9661 aacagcaatg aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg
     9721 aaaaatgggc gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta
     9781 ttccaaacat ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg
     9841 agcaacaggc cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac
     9901 agatgtagtt ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat
     9961 ctgaagcgat ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt
    10021 agccatggtg taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg
    10081 aagaagtatt taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata
    10141 aagtgacgga tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg
    10201 aattcgtttt agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac
    10261 aaattgaaac agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa
    10321 taaaagcaaa gaaggataaa aagctgcatg cagcttttta tccttctttt tttaatcttt
    10381 ttgacacgta aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa
    10441 gtaaagcggt tttctatata attattaata attcattaca caaagataag aataaccttt
    10501 gttataaaac gatttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg
    10561 aagcagagag gggtattcga ttgtccgata accaattatt agaagttcag aacctacata
    10621 caggattccg actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag
    10681 acaaaaacga gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta
    10741 ctacgattat gggattactt gatcctaata acacaaaaat tacaggtgag attttatata
    10801 acgatctgaa tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata
    10861 tcggaatgat tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc
    10921 agatcaaaga aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg
    10981 cactagaatt gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc
    11041 cccatgaact atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca
    11101 aacctccaat cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac
    11161 agattcttga tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca
    11221 ctcatgactt aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac
    11281 agttcgttga agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac
    11341 gctctttact gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg
    11401 aaggtgtcgt tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta
    11461 ttccatggat accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga
    11521 accacttggt acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg
    11581 tgtagatgac agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg
    11641 gattcttcaa tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg
    11701 aaaaaggaaa gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa
    11761 aagccgttgt gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg
    11821 taacgaagcg aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc
    11881 aagattcaat gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca
    11941 tccgaaattt tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag
    12001 atatcgttgg gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac
    12061 aacgtcaacg tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg
    12121 atgaacctgt atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac
    12181 gaatccagca agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa
    12241 aacacatgtg cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa
    12301 gagaagacat ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc
    12361 cgcaaatcga tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag
    12421 agtatatcca aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta
    12481 cgatcacaac tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac
    12541 gattctgcga cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt
    12601 cttattagcc caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga
    12661 tccggcagtg atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca
    12721 gtacttccgt tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa
    12781 acgctctgtt gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt
    12841 gacagttgtc cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca
    12901 aaattcaatc ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt
    12961 atttatcttc ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac
    13021 aagtggatca caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt
    13081 gaaatatatt ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca
    13141 atatctgcga agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc
    13201 caaaggggta ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc
    13261 gatggcttct caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga
    13321 aaagattttt ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga
    13381 ctataccgtg atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt
    13441 actttcagat atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc
    13501 agaaaggaga atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct
    13561 cctatgggga tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca
    13621 ttaggtttgc tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa
    13681 gacaaagtga tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca
    13741 caaggacaaa agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg
    13801 atcataggtg ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc
    13861 atcggggttg gattagggat catctctggc tactacggag ggatcattga caacatcttg
    13921 atgcggatcg ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta
    13981 tcgattattt ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg
    14041 gtagctaaag cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc
    14101 agtgcctcaa aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg
    14161 aatctaagtt cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc
    14221 gagacaactt tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca
    14281 ttgatcggtt atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc
    14341 gcatcaatcc ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag
    14401 cgttctgcag atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat
    14461 gaagaaaacg atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg
    14521 gggcggaggt acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga
    14581 attaactttt ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga
    14641 agctgcggtc gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga
    14701 ttcatatgat gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga
    14761 tttcagaatc acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc
    14821 gacaatcaaa gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga
    14881 tgtgattttc ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga
    14941 cactttccgt aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc
    15001 cggaatcaaa aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag
    15061 catgcttcaa tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg
    15121 tgtagcaatc aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg
    15181 cccttattac atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca
    15241 ctactggaat ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag
    15301 tgcaactgaa gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac
    15361 ttacgatacc tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta
    15421 tacttatcta ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa
    15481 tccagattca aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa
    15541 taatgcagta ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc
    15601 tccagtattc cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa
    15661 agcgaaaaaa ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga
    15721 agacaaagat ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc
    15781 gcaaccattg gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata
    15841 cacaacaggt cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc
    15901 tgaaattgat gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact
    15961 atggggacca aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt
    16021 gatcaacgat atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca
    16081 aaaatggcaa caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga
    16141 agtattgcca gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc
    16201 atgggcaaat gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa
    16261 agggcgatgt cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt
    16321 caaggcgctg atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat
    16381 taagtcaaat ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc
    16441 gaaactaaac gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt
    16501 accttttgac cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa
    16561 aaaatatcca taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa
    16621 aactataaca tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc
    16681 tatgtgaatg agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga
    16741 gatgccgttt tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca
    16801 gaaggaaaat tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt
    16861 gccaaagaat gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga
    16921 ggacgtacgc gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat
    16981 cttgaccagg gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc
    17041 aaagcaggtg ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa
    17101 aaatcaggtg atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa
    17161 cgagtatttt ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa
    17221 tcgaaaaaat tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa
    17281 gtgagggaaa gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga
    17341 caagacagtc attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg
    17401 aaaaagcaat attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct
    17461 gcgaggcgga aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa
    17521 tattgcagaa gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa
    17581 tgagatcagt gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa
    17641 acaaagctgc agattaaaag atatccaaga attgttctta gattctggat taggaaaaga
    17701 atcgttctca atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga
    17761 ccgtcgtggg atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa
    17821 agcagaacag aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca
    17881 cgaattggaa gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt
    17941 acggctaaaa gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac
    18001 agcaaaaaaa gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg
    18061 caaactatct gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa
    18121 tgcgcaagcc gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa
    18181 actgaagcag acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa
    18241 aagctcgcaa gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga
    18301 aaagcttcaa gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc
    18361 tgaagcaaat ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga
    18421 attattggca gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg
    18481 aaacgaactg aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac
    18541 gttagccaag caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga
    18601 attgacacaa aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga
    18661 aatgatccaa caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat
    18721 gtatcaatta atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat
    18781 ccaagaaaac tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca
    18841 gctttcgggt attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt
    18901 agctatcgaa gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga
    18961 tgctcggcaa gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc
    19021 actgacgaca atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt
    19081 agaagggttt atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt
    19141 ggttcaaaac ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat
    19201 cgcgcaaacg atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc
    19261 cggaggatcc atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca
    19321 aaatcaagaa ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc
    19381 acaagaaaaa aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga
    19441 agttttgcgt acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct
    19501 acaaaacatc ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga
    19561 gacacgtgaa ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga
    19621 caaacaaaca gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag
    19681 tcaagaaagc gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga
    19741 acaagcagat cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg
    19801 tgcccgagtc caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc
    19861 gacattgaca gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat
    19921 taacgaatta tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca
    19981 gcgagataga aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg
    20041 tgagcaaaaa gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga
    20101 aatgatttta gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa
    20161 agcttcccaa gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc
    20221 cttaaaagaa cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga
    20281 acaagtcagt gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa
    20341 aaaccaattg tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga
    20401 agtatttgaa gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg
    20461 taaagctgaa ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga
    20521 agtacaacct cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc
    20581 acttacagcc atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat
    20641 cttagatgag gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt
    20701 gagcgatttc caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt
    20761 cgcagcagat gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc
    20821 tgtacggatg gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg
    20881 aaaatgatca aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg
    20941 atttcagatg agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt
    21001 ctatgtacag gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta
    21061 gatgaaggtg actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt
    21121 gaaatcatgg aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct
    21181 cagcaattgg atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt
    21241 gccccaagga agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg
    21301 gatataagcg atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa
    21361 gaagaattag atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc
    21421 atcaaaacaa gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt
    21481 atttctttat tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat
    21541 gaggaaaatg atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct
    21601 gtgcctttcg tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta
    21661 gccaaagtga tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa
    21721 aatcaagcgt gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta
    21781 cgacaagggt ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc
    21841 caattttcga acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc
    21901 agatgtaggt tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact
    21961 cagaaatgcg aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct
    22021 ctatgaagcg gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac
    22081 agttattttg tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc
    22141 ccatcagttc aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc
    22201 tggagcaatc gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg
    22261 caatgcagga ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga
    22321 gcaggcagat gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat
    22381 gaacgaatta gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga
    22441 aacacttctt gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt
    22501 caaagaaaca acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg
    22561 cggtatcgta cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg
    22621 tgaaggaatc gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa
    22681 aggattacta aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca
    22741 gctccttgtt tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata
    22801 cttatttgta agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat
    22861 tagattcata tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt
    22921 caatgcgcgc ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt
    22981 tgatcttgca ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta
    23041 tcacccctat atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac
    23101 cgattctatt cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc
    23161 tataccgaaa gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga
    23221 cgaagagatc attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt
    23281 agcaaaagaa tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt
    23341 tggcgatgag ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga
    23401 attcaaaaca gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat
    23461 cacttattta ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca
    23521 tccgcaagaa cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa
    23581 tgtgaaagtt tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga
    23641 agaagggaaa ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa
    23701 gaaagtcgca gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt
    23761 gaatccttat gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct
    23821 gatgtacatg ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac
    23881 gactcgaaat aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga
    23941 aggtagagaa attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc
    24001 tctttattta gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc
    24061 gaaactctat caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca
    24121 tcaatattat aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga
    24181 actttctact cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt
    24241 agatgagtcg gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa
    24301 tgcaaacatg acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt
    24361 aacgaaaaaa gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc
    24421 catggaagaa gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc
    24481 taaatcaacc aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga
    24541 ttatcaagca ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt
    24601 tatgccggga acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt
    24661 gcgagtccct gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga
    24721 aaaaaatagt gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt
    24781 aggtgaatta gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca
    24841 agccaatcaa attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat
    24901 cgatatgact gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc
    24961 actaggagct aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac
    25021 aactgataaa aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca
    25081 tgtttatcct tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata
    25141 tcctgaagta ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga
    25201 tcaaaagctt tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc
    25261 cagaagcttt ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg
    25321 tatttttttt cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat
    25381 ccgaattcct caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt
    25441 ttcgttgcta gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt
    25501 atggaagcat ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg
    25561 atcatttcag caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta
    25621 tgctgttctt cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt
    25681 acatcatctt gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata
    25741 gcatattccc aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca
    25801 cttaaaataa gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg
    25861 actagtagca ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt
    25921 acaattgtca gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg
    25981 tttgccacga acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat
    26041 catgcaacat ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag
    26101 tgcgcgttta ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg
    26161 atccttgaca gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg
    26221 attgttcctc cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat
    26281 cattggttca gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc
    26341 atggcttcaa gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt
    26401 tgttccattg ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg
    26461 cgaatcgttt gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg
    26521 cttagtatca ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg
    26581 agtgatcatt ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg
    26641 cgtaactgct ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt
    26701 gatttttcct tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt
    26761 cctttatctt ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg
    26821 gtatgcttgt tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa
    26881 actggcattt tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc
    26941 tagctgtcag cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc
    27001 cttcagtatt gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta
    27061 tcggaggagt gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc
    27121 tgatggtcgg gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc
    27181 tttttttatt actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct
    27241 ttttacaagt catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa
    27301 gctttggcaa tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct
    27361 ggtacctgtt atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag
    27421 cagtaaacct cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc
    27481 ttttgacagg gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg
    27541 caaatatcag ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct
    27601 ttctggtagc tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct
    27661 ggaatacaac agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat
    27721 tgattcgcga aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca
    27781 tggaaaatca gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt
    27841 tatcggtcca aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc
    27901 aaaagataca ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga
    27961 attagccaaa aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt
    28021 gaaagaatta gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga
    28081 cgaggaaatc gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc
    28141 gattgacact ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca
    28201 agacacggat tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt
    28261 tcaaatcatg cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt
    28321 tcttcatgat atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg
    28381 acagttattt acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt
    28441 atacgaaatg gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta
    28501 aaactaacat taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat
    28561 catttggttt agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa
    28621 gcagcacaca aactgaaact acattgacga taacagatag taatggagac caaatcgagg
    28681 tgccaaataa tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg
    28741 ctcttggtgt tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt
    28801 catcctataa aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta
    28861 atcagcttca gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc
    28921 taagtcaaat tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt
    28981 tccagcaaaa tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc
    29041 aattagagga attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca
    29101 aaaaagcact agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta
    29161 gattcggttt catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat
    29221 ccactcacgg tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat
    29281 ttgtagtaga cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca
    29341 atgaactggt agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag
    29401 atgtctggta tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga
    29461 atcaagcatt taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa
    29521 tgtttcttac attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc
    29581 gtaaaatcgt attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa
    29641 cgtgaattca ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag
    29701 tgtgatccat tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc
    29761 gaaacttaga taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac
    29821 gatgttccat ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta
    29881 ttttctgtcc agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa
    29941 caatgttcaa tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt
    30001 taggcatgtg tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg
    30061 gcgggaagct tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt
    30121 ttatactcgc ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa
    30181 atatccaatg ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa
    30241 ccctcgatat tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg
    30301 attcctccaa agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc
    30361 cgtgaacaaa aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta
    30421 ttttttgtta tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt
    30481 tttccgttca agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa
    30541 tcataataaa cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt
    30601 agttgcatct gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag
    30661 aagtgacaaa agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt
    30721 tattagatat cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg
    30781 atacggcatt tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt
    30841 ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca
    30901 tggatgatcc atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac
    30961 caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg
    31021 gaaaattggc gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc
    31081 tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag
    31141 atacggctaa taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat
    31201 tatctacgac atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga
    31261 tggaccattt tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga
    31321 ccgttctttt agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg
    31381 aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa
    31441 cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg
    31501 aagcaaaaaa acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca
    31561 tgtatcaatc tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat
    31621 cattcggaga attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc
    31681 atcgtcgctt tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt
    31741 attattatga atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct
    31801 atgatcctat gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc
    31861 aaacacaaca tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca
    31921 gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag
    31981 atccatttat cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg
    32041 atcggatgat ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga
    32101 taatcacaaa aaaaaatata atatacattt tttatttaat tataattaat attgattgaa
    32161 gtctaggcag tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag
    32221 gctatgacag ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc
    32281 aaatcgggct gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt
    32341 tcttttattt cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga
    32401 tatgtttagt aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag
    32461 cagcaatgat gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat
    32521 acccaaagga atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa
    32581 ctcctcctgt tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc
    32641 caagccctgg aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga
    32701 ctcaaaaaat ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg
    32761 ggtctagaaa accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga
    32821 aattaagtgt ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag
    32881 cacagctgaa atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc
    32941 aaacggtgaa ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg
    33001 ctaaatcagg aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa
    33061 atcaagatgc ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt
    33121 ccactaaatt agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact
    33181 gttaaaaata aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag
    33241 gctaggctaa atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa
    33301 aagatatatt gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata
    33361 tctttccgat catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag
    33421 acgaagaaag caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag
    33481 aaagcagtga ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg
    33541 agggttcgga caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg
    33601 atcccaatat tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca
    33661 tattggaaaa tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta
    33721 tttggtcaaa taagaagatt agtttactct cggattttac atttaaatct tatttatact
    33781 taggaaatca acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta
    33841 gaatggctag taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt
    33901 ctactcgttc tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata
    33961 aaaatagtgg ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg
    34021 gacatgtcgc ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg
    34081 gcgtgacggt agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat
    34141 gggacgcgga gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga
    34201 ttatacctga tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt
    34261 caacaggtac tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca
    34321 catcaacagc tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag
    34381 gaaacgataa gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata
    34441 agaaaatgca gactagtatc gacctcccac aagaattaac tattcccgag catatcttga
    34501 tcaatggaaa tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa
    34561 atctgacaga ttattttatt gctggagata cgttaataat cgaattggag acaactttgc
    34621 aggaagtaat tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt
    34681 tgattgagca atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc
    34741 atgtttatta taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg
    34801 ggaaaatagg agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact
    34861 cggattcagc gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt
    34921 acagaaaagg caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata
    34981 aaatatccag tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag
    35041 atgaacgggc ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg
    35101 gagattttga gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag
    35161 ggacatccgc tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc
    35221 ttttagattc tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa
    35281 tcggaacctt tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg
    35341 aagagaagaa tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt
    35401 gtgtcccaca gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat
    35461 tttcttgaga ccaaaacaac aattatttat aacactccta gttattttag gggcactttt
    35521 taacaatata tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga
    35581 gaatcaaatc gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt
    35641 agaagtaaaa gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt
    35701 gaatagcgca accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa
    35761 agacatgagc ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct
    35821 aaaacctaaa agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga
    35881 tggtgtattg gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga
    35941 aactaaggat caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat
    36001 gcaacagaca ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca
    36061 aatcaatgca agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa
    36121 tcaagtagtt attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga
    36181 ggaaaaagaa ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa
    36241 tggtcaaccg ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa
    36301 tgaaaatgga aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg
    36361 ggtatttgaa aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga
    36421 tgttacgatt aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt
    36481 gtttatttta ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta
    36541 aggttaagat aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa
    36601 caatataaaa tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa
    36661 taacaggttt gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga
    36721 caggacgatg tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa
    36781 tcagttttat ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg
    36841 atgactaatg aattatgagt tttttttaag caaagaagat cacagaagtt attcattact
    36901 taaatattta gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt
    36961 gtcgctatcg acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa
    37021 gttcgatcta gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat
    37081 taatggaaaa tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat
    37141 gaaaatggta ttggctttct ttcaggaaaa atatgtctca gtagaaaatt ttgctaaaca
    37201 gcatcacgtg agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa
    37261 aaaatatcaa attttttttg aaaagagaaa gtt
//
LOCUS       DACEDM010000023        36706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEDM010000023 DACEDM010000000
VERSION     DACEDM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.023"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            647..964
                     /locus_tag="IWO50_001032"
     CDS             647..964
                     /locus_tag="IWO50_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0358169.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IWO50_001033"
     CDS             complement(1055..1420)
                     /locus_tag="IWO50_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358170.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IWO50_001034"
     CDS             1628..2110
                     /locus_tag="IWO50_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358171.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IWO50_001035"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IWO50_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0358172.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3484..4758)
                     /locus_tag="IWO50_001036"
     CDS             complement(3484..4758)
                     /locus_tag="IWO50_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0358173.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5031..7502
                     /locus_tag="IWO50_001037"
     CDS             5031..7502
                     /locus_tag="IWO50_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0358174.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7605..9341)
                     /locus_tag="IWO50_001038"
     CDS             complement(7605..9341)
                     /locus_tag="IWO50_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0358175.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9338..10042)
                     /locus_tag="IWO50_001039"
     CDS             complement(9338..10042)
                     /locus_tag="IWO50_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0358176.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10173..10676
                     /locus_tag="IWO50_001040"
     CDS             10173..10676
                     /locus_tag="IWO50_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0358177.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10636..10974
                     /locus_tag="IWO50_001041"
     CDS             10636..10974
                     /locus_tag="IWO50_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0358178.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10995..12413
                     /gene="ffh"
                     /locus_tag="IWO50_001042"
     CDS             10995..12413
                     /gene="ffh"
                     /locus_tag="IWO50_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0358179.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12525..13103
                     /locus_tag="IWO50_001043"
     CDS             12525..13103
                     /locus_tag="IWO50_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0358180.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13107..13628
                     /locus_tag="IWO50_001044"
     CDS             13107..13628
                     /locus_tag="IWO50_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0358181.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13707..14261)
                     /locus_tag="IWO50_001045"
     CDS             complement(13707..14261)
                     /locus_tag="IWO50_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0358182.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14514..14789
                     /gene="rpsP"
                     /locus_tag="IWO50_001046"
     CDS             14514..14789
                     /gene="rpsP"
                     /locus_tag="IWO50_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0358183.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14801..15052
                     /locus_tag="IWO50_001047"
     CDS             14801..15052
                     /locus_tag="IWO50_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0358184.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15177..15968
                     /locus_tag="IWO50_001048"
     CDS             15177..15968
                     /locus_tag="IWO50_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358185.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16138..16659
                     /gene="rimM"
                     /locus_tag="IWO50_001049"
     CDS             16138..16659
                     /gene="rimM"
                     /locus_tag="IWO50_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0358186.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16649..17410
                     /gene="trmD"
                     /locus_tag="IWO50_001050"
     CDS             16649..17410
                     /gene="trmD"
                     /locus_tag="IWO50_001050"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0358187.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17546..17893
                     /gene="rplS"
                     /locus_tag="IWO50_001051"
     CDS             17546..17893
                     /gene="rplS"
                     /locus_tag="IWO50_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0358188.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            18151..18444
                     /locus_tag="IWO50_001052"
     CDS             18151..18444
                     /locus_tag="IWO50_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0358189.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            19287..21614
                     /locus_tag="IWO50_001053"
     CDS             19287..21614
                     /locus_tag="IWO50_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0358190.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            21740..22069
                     /locus_tag="IWO50_001054"
     CDS             21740..22069
                     /locus_tag="IWO50_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0358191.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            22109..23113
                     /locus_tag="IWO50_001055"
     CDS             22109..23113
                     /locus_tag="IWO50_001055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0358192.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            23110..24539
                     /locus_tag="IWO50_001056"
                     /pseudo
     CDS             23110..24539
                     /locus_tag="IWO50_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            24745..25917
                     /locus_tag="IWO50_001057"
     CDS             24745..25917
                     /locus_tag="IWO50_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0358193.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(26079..26534)
                     /locus_tag="IWO50_001058"
     CDS             complement(26079..26534)
                     /locus_tag="IWO50_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358194.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            27124..27546
                     /locus_tag="IWO50_001059"
     CDS             27124..27546
                     /locus_tag="IWO50_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0358195.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(27712..28905)
                     /locus_tag="IWO50_001060"
     CDS             complement(27712..28905)
                     /locus_tag="IWO50_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0358196.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            29129..29506
                     /locus_tag="IWO50_001061"
     CDS             29129..29506
                     /locus_tag="IWO50_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0358197.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            29494..29832
                     /locus_tag="IWO50_001062"
     CDS             29494..29832
                     /locus_tag="IWO50_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0358198.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            29966..30409
                     /locus_tag="IWO50_001063"
     CDS             29966..30409
                     /locus_tag="IWO50_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0358199.1"
                     /translation="MLDPVDTGKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            30671..31486
                     /locus_tag="IWO50_001064"
     CDS             30671..31486
                     /locus_tag="IWO50_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0358200.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            31496..32158
                     /locus_tag="IWO50_001065"
     CDS             31496..32158
                     /locus_tag="IWO50_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0358201.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            32363..34027
                     /gene="alsS"
                     /locus_tag="IWO50_001066"
     CDS             32363..34027
                     /gene="alsS"
                     /locus_tag="IWO50_001066"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0358202.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            34040..34750
                     /gene="budA"
                     /locus_tag="IWO50_001067"
     CDS             34040..34750
                     /gene="budA"
                     /locus_tag="IWO50_001067"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0358203.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            34880..35377
                     /locus_tag="IWO50_001068"
     CDS             34880..35377
                     /locus_tag="IWO50_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0358204.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            35562..35822
                     /locus_tag="IWO50_001069"
     CDS             35562..35822
                     /locus_tag="IWO50_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358205.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(36184..36423)
                     /locus_tag="IWO50_001070"
     CDS             complement(36184..36423)
                     /locus_tag="IWO50_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358206.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT        12339 a         6418 c         7307 g        10642 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt
     3481 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
     3541 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
     3601 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa
     3661 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
     3721 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
     3781 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
     3841 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
     3901 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
     3961 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
     4021 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
     4081 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
     4141 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
     4201 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga
     4261 tttgaactca tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt
     4321 cgttgcttca cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat
     4381 tagtttacgt gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt
     4441 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg
     4501 gcagagaaaa cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat
     4561 ctgagtgcgt gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca
     4621 ataggaagaa gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc
     4681 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
     4741 tctagtatga ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat
     4801 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
     4861 ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg
     4921 aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga
     4981 taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata
     5041 aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc
     5101 gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa
     5161 aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg
     5221 aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag
     5281 tagaatccta taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca
     5341 tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa
     5401 aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag
     5461 atgacattgt tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag
     5521 ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa
     5581 taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag
     5641 acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag
     5701 aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca
     5761 tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga
     5821 tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt
     5881 acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc
     5941 ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata
     6001 aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac
     6061 aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga
     6121 tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc
     6181 aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg
     6241 cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat
     6301 atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt
     6361 acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc
     6421 tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc
     6481 aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc
     6541 aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat
     6601 atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa
     6661 tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     6721 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     6781 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     6841 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     6901 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     6961 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     7021 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     7081 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     7141 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     7201 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     7261 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     7321 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     7381 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     7441 agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt
     7501 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     7561 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     7621 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg
     7681 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt
     7741 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     7801 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     7861 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     7921 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     7981 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     8041 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     8101 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     8161 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     8221 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     8281 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     8341 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     8401 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     8461 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     8521 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     8581 tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc
     8641 atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc
     8701 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     8761 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     8821 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg
     8881 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     8941 gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     9001 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     9061 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     9121 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     9181 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     9241 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     9301 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     9361 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     9421 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     9481 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     9541 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     9601 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     9661 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     9721 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     9781 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     9841 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     9901 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     9961 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
    10021 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
    10081 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
    10141 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
    10201 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
    10261 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
    10321 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
    10381 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
    10441 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
    10501 gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg
    10561 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
    10621 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
    10681 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
    10741 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
    10801 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat
    10861 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
    10921 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
    10981 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
    11041 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
    11101 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
    11161 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
    11221 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
    11281 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
    11341 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
    11401 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
    11461 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
    11521 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
    11581 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
    11641 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
    11701 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
    11761 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
    11821 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
    11881 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa
    11941 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
    12001 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
    12061 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
    12121 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
    12181 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
    12241 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
    12301 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
    12361 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    12421 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    12481 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    12541 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    12601 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    12661 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa
    12721 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    12781 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    12841 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    12901 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    12961 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    13021 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    13081 gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt
    13141 tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag
    13201 gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa
    13261 aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga
    13321 atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag
    13381 atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg
    13441 gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc
    13501 agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag
    13561 cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac
    13621 gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt
    13681 tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac
    13741 gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca
    13801 attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa
    13861 aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat
    13921 gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct
    13981 acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa
    14041 acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt
    14101 tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt
    14161 tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct
    14221 tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa
    14281 actatctata ataatcttac aaaagataag taactggagc aaataaaaac acttactgac
    14341 aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta
    14401 ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa
    14461 aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag
    14521 ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag
    14581 ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt
    14641 tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag
    14701 gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac
    14761 accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt
    14821 agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt
    14881 agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat
    14941 cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat
    15001 caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt
    15061 gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt
    15121 atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga
    15181 gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc
    15241 aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa
    15301 aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga
    15361 aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg
    15421 taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta
    15481 ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa
    15541 cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag
    15601 ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca
    15661 cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc
    15721 gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa
    15781 tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg
    15841 ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg
    15901 cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga
    15961 ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa
    16021 tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag
    16081 aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg
    16141 actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt
    16201 gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta
    16261 ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat
    16321 tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat
    16381 ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac
    16441 catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa
    16501 atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata
    16561 ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt
    16621 gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag
    16681 aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt
    16741 agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga
    16801 ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat
    16861 ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc
    16921 tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt
    16981 catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt
    17041 ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc
    17101 aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta
    17161 ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa
    17221 agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga
    17281 atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc
    17341 tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga
    17401 agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta
    17461 tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa
    17521 tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca
    17581 attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt
    17641 tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg
    17701 tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg
    17761 tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt
    17821 acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga
    17881 aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc aagggttctt
    17941 ttttcttagc atgtaatttg aacaccctga tccagttttt tctttaatta ttggtagggg
    18001 agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga gcactatgat
    18061 aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta acataaatgg
    18121 agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga gcaagaaaag
    18181 atgaaagttt tagacacatt catgaatgat ggaaaagtaa aacaagtacc aagttcaaca
    18241 aaaaagaaat acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg agtgacttat
    18301 acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga ttatgtcgaa
    18361 caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg tcgggcatat
    18421 cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa aagttcaagt
    18481 atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta tctataatac
    18541 aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg agcgattcgc
    18601 agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa aatcaattgg
    18661 atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac caaaacgtac
    18721 ctcctaaaat tagattttgg acagatgccc tttaaaaaga taacaagatt tcgttcttga
    18781 aacaatatat atgcttttat tccaatgagg ttgtgaaaac agtattttgt cctgagcgtt
    18841 agaaaattga aaatggggaa tagcaactcg tactttacca ttttttaagt gaatacaaga
    18901 tcagctaatt gaacaccgtt tattcgtgat gagggacaca acactgcagt cgacacattt
    18961 ctcttttatg ttcaaagata acaaatattt aaaacaaaga aaaatagtag ataatcccgt
    19021 cctaactctt ttattcttcc ctcatagctt ccaaatttaa gcaatacttc aattaatcaa
    19081 gttttatctt tttttttcaa taatcgtaaa aaaagagtat tttttattta aaaataaaat
    19141 ctttgtatta aaggacaaga ctaaaataac cttagaataa aaagagaaaa aaagccccaa
    19201 actttctata catcaataaa ataataaaaa ttaaatttag aataacgtgt aaaggattat
    19261 ttgatgattt tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc
    19321 aacgtatatg tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact
    19381 tacatcccat tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta
    19441 tttatgtaaa cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa
    19501 atagtccacc ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg
    19561 agacaacctc aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag
    19621 cagcaaaagc agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta
    19681 ctgttgcggc cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat
    19741 atgccaaaga tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt
    19801 tacaaggcgt aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt
    19861 ttattggctc cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg
    19921 tggctaccgc tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt
    19981 ttacaccctc aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg
    20041 gtatacagtt agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg
    20101 ttgatggtaa gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca
    20161 atgcgaatcg gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa
    20221 cgtacgggac tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag
    20281 cagtaacggg ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg
    20341 tcgatacggt gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt
    20401 ttggacagat gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag
    20461 acctgacgac taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgacag
    20521 aaacaaagaa ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa
    20581 tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg
    20641 caacagtatc aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga
    20701 tcgttaaata tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca
    20761 gtattgatca aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg
    20821 aagtaactgc gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac
    20881 tagctaaact agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct
    20941 atacgattaa gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa
    21001 aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc
    21061 cagtacaagt ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata
    21121 tggttaatgc tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag
    21181 cagcaaatcc caagttgtca tttactgata tagccaacac atcaggaact tataccatct
    21241 ataaagctaa cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc
    21301 actcgatttc ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt
    21361 ataccctaac agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag
    21421 gcgaaggaag tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga
    21481 tcaatccgac tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt
    21541 taggactgat ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag
    21601 gtgaacagag atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt
    21661 tatcctactg gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac
    21721 atttactttg cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga
    21781 cggaataaat atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc
    21841 tatatatgat gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga
    21901 cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac
    21961 aaaaacagta gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca
    22021 aggtcgtgac gctgtttacc gatttgcgga aataaagact tcagattaag taaaagaaaa
    22081 atcagcacca tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac
    22141 catccacttg tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac
    22201 agtagagaat aagcagacag attttgcaga tggggataaa gttccttatc tcatcaccgt
    22261 aacaattcct gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc
    22321 tcagttatgg ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac
    22381 atttcacaca caaaaaacaa aacatggttt tgtcttatca gattctggaa aagatcttag
    22441 ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga
    22501 aaaaacgaca tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca
    22561 tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa
    22621 actagcagat gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg
    22681 gataaatcat tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga
    22741 tggaacattt tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc
    22801 gcctaaagga tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta
    22861 tgatccgctt catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa
    22921 taatggaaaa acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg
    22981 aagctttcca aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttgttgct
    23041 agtcctttca acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag
    23101 ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg
    23161 ctgcttcctt tcgttctcgg cctaggaact gcagaggttt ctaggccgta tcacctacac
    23221 cagaaaatgt tacggttaat ttgcataaat tgaaattcac ttcagcacca gaaaatcaaa
    23281 taaacaacgg aacggaattg acatttccga atagcgaacc gttgaacggc gttgaattca
    23341 acgtatatga catcactgct acgtactatc caagtaaaga cacagcagtt ccagcagacg
    23401 ctacaccctt cgcaagtgta acgacttctg gtgaaggatt agctaacctg actcttccag
    23461 gtaaatctga tgggaaagat gctgtatacg tctttgtaga aacaccaaag cccggtgtcg
    23521 aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa cccgaattca tcagaccctg
    23581 acaaaccttt agacacgatt cacctatatg ctaaaaatgt catcaaagaa agcaatatca
    23641 aaatccagaa agctgggaac ttttcagggg tagataagtt agcaggggcg caattccaga
    23701 tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga aaacactggt gtcgctctct
    23761 atgatcctgc tgagtcagcg ttgacattca caactgatac tgaaggatcc atcagtgtca
    23821 atggattaat agacggcaat tatactttag ttgaaacaaa agcaccagat ggttatcaat
    23881 taaccaatcc acaaaaagaa actactttta tagtaaaaaa cggaaaattg caacaaacaa
    23941 ccggtatcat ccatggcgca gatgaagcca atcttgggaa tccaaacggt gtagtcaatc
    24001 gggaaaatac aactacggat aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt
    24061 tccacttcat caaacaagat gtgaacacgc aagaaaaatt agccgatgca gaattttcta
    24121 ttctaagagc aaaagatcca actgttaaat taatgaaaaa aatagcaaat cttgcctcag
    24181 gagaataccc atatgcttgg tcagatgaag tcacaggttc tgactgggaa gaagtgaaat
    24241 tgatttctga ttcagaaggg aaattttcaa tcgaagggct gaaacaaggg gcatactgcc
    24301 ttcaagaaac aaaagcacca gaaggctacg cattacctaa gaatgaagct gcgtacactg
    24361 aatttacagt agatgatgat ccaacaactt ctgatgaaac aacgatcgat aaccaaccta
    24421 aaggaatctt gccttcaact ggtggtatgg ggatcatcgt attcatcttg gtaggtactg
    24481 ctttagttgg aggagcagtg atctacttca aaaaacgcca tcaagaaaca gaagcttaaa
    24541 ctattggaaa caaaaatcat acgataaata aacgatagtg attcaaatgg ggagaaaggt
    24601 tgtgacaagt ggtcggtctc ttggtattcg ctcaacaaac ggcaaaatag gaggaactat
    24661 tttgccgttt gttgagcgaa tactcaggtc aaggcgctga tttcacaacc tcttcatata
    24721 catattagaa aataggtgga catgatgaat aaaagcagaa taagaatact ggacatcttc
    24781 atggcaatca ttttagtcgt tgggatcggg atcttttctt atccttttgt agaagattca
    24841 ctgaatgatt ttcttgccca gcaaatgatc atccattatc aaaagcaagc atcgaataag
    24901 aattcagagg aaataaaaaa acagcaggaa aaaatgacaa agaaaaacca acagttggca
    24961 gagcaaaacg tatcaccagg gataggcagt ttcaaccaag cagttgatgc aaaagcatta
    25021 aaagatttac ctagtaatgc cttttttatg gaacatatgc ttggagtcat cgaaatccca
    25081 aaaatcaatg tcagcttacc tatattcgat cagacaacgg aaatattttt gcaaaaagga
    25141 acgtcattat tggaagaaag ttcttatccg actggcggga aaaacaccca tgccgttctt
    25201 tctgggcatc gtggactccc agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa
    25261 ggagaccagt ttttcatcca gatcaatgga aagacattgg cgtatcaagt agaaaaaatc
    25321 caagtggtct tacctgatga agtagattct ttgggtatcc aaaaaggcag agatctcgtg
    25381 actttgctta cctgtactcc ttatatggtc aatacacatc gactgcttgt gacggggcat
    25441 cgtattcctt atcaagcaaa agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa
    25501 aaatggaaat tttttatttg gtttatcggt atactacttg gatcaatcgg cttagtttgg
    25561 ctactcatcg cttatttaga ttctttggcc atcgctaagc gaaactatcc tttatctttt
    25621 tatgtaaaga acacaaacgg acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc
    25681 aacggcaaac actatattac tagagaaaaa gttccatttg tgaaagcttc tgatgaatac
    25741 ggactagtga gattttctga tttaaaaggc aggaactatc gcttgcaaca tgaggagctc
    25801 ctgcttaaaa tccatgtaaa acataaacat tcgaaacagt tttcaatgaa attgaaaaaa
    25861 ggaagatata aactgcgaaa agaaaaagag gtgtactatt tgattgagaa agaatgaatg
    25921 aagatgtcca aaaaagcaga gggaacagac taaatgaaaa aagagagaag ctgacgaaat
    25981 ggacgtgtaa actgctattt ttggtttact atctggaacg ccagcttcta tttttttatg
    26041 tttcagaaga attttattat tatagtataa cttttgaatt agtttccata acatttggag
    26101 caaggggtta aaccacgagc ttgagcttcg cttaaagtgg caggggtata cgtaccattt
    26161 ccacatttat gtgtatggta ttttgaaccg gtcggtgtga catagacagt ttgttcatta
    26221 ttttgctcag gagctgcggc ttgtgcctgc tgagaggcct gttcagctgc tgcttgctgt
    26281 cgcgcctgtt ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt
    26341 cgtgcaattt cttcttgctt tttccgttct tcttctgctt cttttgcttt gattgtggaa
    26401 tcaattgcag caagacggct tgtcaactct tgtttgtttt caggtagcgc ttggatagct
    26461 gctgaagcag ttgcatagtt ttcttttgta ggatttgctt cagcttgtga caaagcagat
    26521 tccgcttctg tcactttttt ttctagtgca gctttttccg ctttttgttt ctcttcttct
    26581 tttttctttt caatttgttc ttgttctttc gatgaatctg ccgtactttc ggagatcgtg
    26641 gtactgctgc tggaagcctg agctgaactt gtgctttgag tagtagacgt agtagcacca
    26701 cacccactca acaaacttcc tgcgaccaaa aacaatgcaa ctaatttttt cactaaattt
    26761 cctccttaag taaaaaacaa ccgttttcaa taaatgtcct tttcaattgt ttacttactt
    26821 gttataataa ttgtgttatg aatagtgatt gaggaaactt aatctaatcc cgttttgata
    26881 cacgggattt tttgctatag ttctgttatt aagtatgcaa taataaaata tgtacgttat
    26941 tttattatta gattattgac atttatataa taaacgatta aatgattcat atcaatagaa
    27001 tacttaggaa aaaaagagaa ataataacag gtttatgcac tattgacggt gtgacaatat
    27061 gatatttgta tccgtttttt ttcttgtatg tagtatagta aagtagatac aggaggagaa
    27121 aatatgcaga cttttttaac aaaagtttct caaaagaaaa ttaaaatgat tcatttactg
    27181 cttgaaacag aacgttggtg ttcaatagaa gaactacaaa acgaattgaa ggtatcaagt
    27241 aagtctatct tgaattatct aacagattta gaagaattgt tccaaaaata tcctgataag
    27301 gtcattttga agaatgagaa taatcggcgc ttttctatgg aaaagcagga gaacttccca
    27361 atctacatta tttatcttca tttctacaga aaatcatata attatcattt gattgaattc
    27421 atgtatcaac atcctgagaa aaatttggaa gattatgcag acgctcaata tacgagtgta
    27481 tccacagttt ttcgttatgc taagttatta gtaaagtact ttgaaatgac ggacgtcaaa
    27541 gaatagtagc cgccagatag aaggaaatta gcagttgaaa aagctgccct gcatcaagta
    27601 atacgaccag ccaaacggaa acagtgtctc atgtttttga tttggctggt cttattaccg
    27661 cagatgcaag cttttgccac acctctttct tgtcatttac agactcaact atcaatgctg
    27721 caagctttcc cttaatgctg taacaccagt aagagttgtc aagaccagca gactgactag
    27781 gtaaataccg ctcaaaaaca agattacggc agttaaacta taatgatcca tcaagaaacc
    27841 gatgacgata gaagaaaaac caccaacagc tcgtccaagg ttcataatga aattattcgc
    27901 cgtcgcgcgg atttctgttg gatacaaact actaatcact gcgccatatc caccatacat
    27961 gccattgata aagtagccga ctatcacagc ggctgctacc aaactccact ggctatgggc
    28021 taacacaagt agatagacag ataaggatga acaaattaaa aacaaagaaa aggctagttt
    28081 aggccccaat ttatccatga ttatgccaaa agtcatcatc cccaaagaca tgcctaaaat
    28141 agtactgacc atccacaatg aagacccaga aaccgaaagc cctaatcgat tttgcataat
    28201 tgaaggcagc caattcatta acccaaaata gccggcaatt tggacagtta ccatgatact
    28261 aaggccacat gtttgccaag caagatgttt tgattcaaac agtctgctaa gtttggtttt
    28321 cttttcggca gattgtacag atttgaattc ctttggttca gtcaagccgc gacgaatgaa
    28381 caagaccatc aacaccggca gtacacctac gacatacaac atcttccaac caaacgttgg
    28441 aatgatcaca gcagctaaaa tcgcagccaa agccgcaccg acctgcgcac caataccagc
    28501 aaccgaagaa gctcgtccaa tttgtttttt tgaaaaactt tcggaaatca aggacataca
    28561 cgccccgtat tctcctcccg ctcctacacc tgcgataaag cgaaagagat aaaccaagta
    28621 gatgtttgaa gcaaaataca tcaataaaga agcaatcgaa aacagaagga ccgtttgaga
    28681 aaatactttc acacgtccat atttatcagc tatgatgcca aaaaaaatac ctccaatgag
    28741 catccccaaa ttagtgatcg ttgaaatgaa acccgcttgc gtaccactaa tatgaaaaga
    28801 agtaatcatt gaagataatg aaaaagctaa aaacataata tccatattct ctaaagcaat
    28861 tcccgccgat gtcgataata ataactttcg ctgatacgtt gtcattttta tacccctttt
    28921 cctaaaattt ccctaaattg ttaaaagtat atccaaaaaa gaataaaaaa acaagacttt
    28981 tttatttgaa ataatacaca aacaaccgat atagataatc cagcttagtt ttaatagaaa
    29041 tgagtattga cttggagtac acttaaagtt gtagaataaa taaaatacat aagtaaagtg
    29101 gcaagaaatt aagagaaaga gtgatcagat gaatatcaaa caagtcagtg aagaaaaggg
    29161 catttcggcc gatacattgc gctattatga acgaatcggg ttgattccac cagttaatcg
    29221 gacaaaaggc ggaatccgtg attatacaga agaagaccta aaatgggtcg actttacgat
    29281 ctgtatgcga aatgccggat tatcgatcga gtctttgatt gaatatattc gcctatctag
    29341 cgaaggagaa gcaactgtat ttgagcggcg taagcttttg attgaagaaa gtgaactttt
    29401 gacgaaaaaa attgctgaaa tgcaagaatg tttggaacgt ttacaaggaa aaatcaagaa
    29461 atatgatcgt gtgttatcta gaaataaaat ggaatgtatc aagtaaaaaa atcaaggagc
    29521 atcgctatgg agttacgctt actaaaatat ttttggacaa ttgcagaaga aggaacaatc
    29581 tctcaagccg cagaagttct acatcttaca caaccaacct taagccgaca actgaaggag
    29641 ttagaagagg aactaggaac agaactgttt gtacgcgaaa accgcaaaat gatcttgaca
    29701 gaagcaggtt attttctaaa aaagccgagc agaagaaatt ttagatttaa cgaatcaaac
    29761 cactcaagaa ttcgagaaca gaaaaaaaca attattcagc ggccgattta cgatcggctg
    29821 tgtggaagct gataattcga atacattggc actattacta gaaaagttga tccgtgatta
    29881 tcccagcgtt caatttcata tccacagcgg aacaagtgat gatatcacgg agtgactaga
    29941 aaaaagcctg ttagatttag cgattttgct agatccagta gatacaggga aatacgaaac
    30001 gattttgtta ccaagaaagg aaaaatgggg attgctcatt ttagaaaatt ctgttttagc
    30061 aaaaaaagac gtgataacaa aagaagactt tgcgaatgta cctcttttga tctcaaagcg
    30121 tccagaaata cagcgaatgt ttgctgactg gttaggcata aaaaaggagc agttgctgat
    30181 cacaggaacg tacaatttga ttttcaatgt atttgcgcta attgagaatc aggtaggtgc
    30241 tgctttagtc atagaaggtg ctgtaagtca tctttcaaag cgagtgacat tcgttccgct
    30301 tgacccaatg attgaaacga actgcatgct agtctggaaa aagaatcgga ttttatcacc
    30361 tgtagtcaat gaattgattc accgtttcaa agaagctttt gaacagtaat aagtatctct
    30421 aatcgaagaa atttcaatac aaaagcgaga cgtatgacag tcttgctttt gtattttttc
    30481 atttttaaat caaaaaaaat aaagagattt acataacttg ttaaataagc gcttacaaac
    30541 ataagaagaa cggaaatgaa tcagctataa taaccgttgt tctggattgt aatcattaca
    30601 aagaaatgaa gattataact ttggaggtgt tcgaaatgaa agaaaaaaag caaaaattcg
    30661 ttcaaaaaac atgcccttta ttcaatggtt gggtatgatt ctgctgattg tattagcttt
    30721 attgacgatc attccttttt tatggatggt ttctgcttca ttcaaagcaa ataatgaagt
    30781 gttcacgatt ccgattcaat ggatccctaa aacttggcat ttagagaatt atctggttat
    30841 ttggtctcgt attcctttag tgacattttt taagaataca gcttttttaa gcgttgtgat
    30901 cacgttgatt cagttgttta cttctagttt tgctgcttac gggttttcta aactaaattt
    30961 taaagggcgc gatattttat ttttagcata tatcggcacg attgctgtcc cttggcagtc
    31021 ttatatgatc ccgcagttca ttatgatgag aaaagtgggt ttaacatata cgttgtggtc
    31081 gttggtgcta ttacaagcgt tcaatgcttt tggtgtgttc ttgttgaagc aatactacag
    31141 tagtatccca gattctttat gtgagagtgc gcggatcgat ggcttatcag agtggggaat
    31201 ctatcgcaag atcattttac ctcttacaaa accggcgatt gccagtttga cgatcatcac
    31261 attcgttaat acatggaacg attatatggg gccattcatt tatttgtctt ctacggaaaa
    31321 taaaacaatc cagttagggt tgaaaatgtt tattgggcta tttgatgcag agtacgcatt
    31381 aatcatggct gcctcagttg tttctgttct tcctgttgct tttatatttt tagcgatgca
    31441 aaaatatttt gtagaaggga tcgcttcatc aggaatgaag gaataggagg tgattatgaa
    31501 agagcagcgt tttgacaaaa atgtttatat ggaattcttt tattggttgt atcaatcatt
    31561 ggcaagtagc ctttgttttt ggtgtgtcaa tatcttgtat atagcagctg cgttcctttt
    31621 gtcgatagat ggaaggaatg tcttttcctt tattttttct ttacttttta ttggtccagg
    31681 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
    31741 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
    31801 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
    31861 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
    31921 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
    31981 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
    32041 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
    32101 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
    32161 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
    32221 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
    32281 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
    32341 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
    32401 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
    32461 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
    32521 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
    32581 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
    32641 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
    32701 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
    32761 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
    32821 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
    32881 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
    32941 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
    33001 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
    33061 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
    33121 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
    33181 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
    33241 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
    33301 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
    33361 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
    33421 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
    33481 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
    33541 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
    33601 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
    33661 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
    33721 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
    33781 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
    33841 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
    33901 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
    33961 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
    34021 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
    34081 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
    34141 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
    34201 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
    34261 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
    34321 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
    34381 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
    34441 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
    34501 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
    34561 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
    34621 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
    34681 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
    34741 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
    34801 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
    34861 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
    34921 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
    34981 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
    35041 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
    35101 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
    35161 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
    35221 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
    35281 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
    35341 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
    35401 aagagaaatc aagaataaaa agagccggaa agacagtggg catttctgtt tttcggtttt
    35461 tttatttttt tataaaatca caagtgttat tgattttcat aatcatattt taatggtatt
    35521 atcattttaa ataatggaaa aacattaagg atggatgaaa tatgaataac gaaactatca
    35581 ttttaggaaa aaaatatatt tgccaaccta ttggcttgaa acaaaaagtg gttggagaag
    35641 taatcagtaa attagaaaat tgtgttgtga tctgcgtaga cgaatatcat gacgtcgatc
    35701 gcaatgaaat attagaaaaa tgcggaaaag tagtggcaaa atatactcac gtttatgagt
    35761 tggaaacaac agaatatcta tataactata gcaacaaata tgaacatact ttagtttcat
    35821 aaagatttaa ataaagtgcc ttcaaaagcg agaagaccaa gcttttgaag gcactttatt
    35881 tagttggcag tgattgttca tgatttttga tttattcaaa aggttcgcct agagaatctg
    35941 tctctgtaac gaggcctggt ggagaaactc gataaaaacc tatactgcca gaactgtcct
    36001 attttcggat agacaaacta gtagttttaa aaagatcatc cgatccaatc atttcaaaaa
    36061 atgcaaaact gatatcagga tcatttagct caggcttgtt atcgtaaagc atttgtaaag
    36121 gttgcattta gttaggagta aacgcgaaac tatcatcatt atttgctgaa gaagatgaag
    36181 aagctaattc agagtttgtt tgaaataaag gagacgtgct gacagtttct ttaccctgtt
    36241 ttttgcagag gtagaaactt ttatatcgga agtttctacc ggagaatttt tctcatttgt
    36301 agaaattgtt gaatcgatac tattcgaaat ttgcgtatct gaaggtatgt tctttgatgc
    36361 agaagtccca caagcagtaa gtaacaataa atttgataaa ctgataaaca gaattctttt
    36421 cattatgtac acactcctta tagtatcgtt tagcatacac ctatgctcag tattaggcaa
    36481 aatcaatgca aaactatcaa gtcttttata gtatctatta gtcttagtaa tacatatatg
    36541 gagagggtga tatcccagtt caaaccagat acattggtta ccatctgtat taacgattat
    36601 gcctctaaac gcttttttta tgaaaaaatt tataagtgaa aacgtaggta tgatattatc
    36661 tagaggtaat ttttttgggt atttaaatag ttgctaggag gatagc
//
LOCUS       DACEDM010000024        35882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEDM010000024 DACEDM010000000
VERSION     DACEDM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.024"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            31..1152
                     /locus_tag="IWO50_001071"
     CDS             31..1152
                     /locus_tag="IWO50_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAQ0358207.1"
                     /translation="MIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IWO50_001072"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IWO50_001072"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0358208.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IWO50_001073"
     CDS             2728..3003
                     /locus_tag="IWO50_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0358209.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IWO50_001074"
     CDS             3009..3776
                     /locus_tag="IWO50_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0358210.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IWO50_001075"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IWO50_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0358211.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IWO50_001076"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IWO50_001076"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0358212.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IWO50_001077"
     CDS             7920..8222
                     /locus_tag="IWO50_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0358213.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IWO50_001078"
     CDS             8225..9388
                     /locus_tag="IWO50_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0358214.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IWO50_001079"
     CDS             9414..12842
                     /locus_tag="IWO50_001079"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0358215.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IWO50_001080"
     CDS             12863..13987
                     /locus_tag="IWO50_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358216.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IWO50_001081"
     CDS             13977..14414
                     /locus_tag="IWO50_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0358217.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IWO50_001082"
     CDS             14398..14736
                     /locus_tag="IWO50_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0358218.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IWO50_001083"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IWO50_001083"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0358219.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IWO50_001084"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IWO50_001084"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0358220.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IWO50_001085"
     CDS             15893..16936
                     /locus_tag="IWO50_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0358221.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IWO50_001086"
     CDS             17095..17823
                     /locus_tag="IWO50_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0358222.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IWO50_001087"
     CDS             17939..18184
                     /locus_tag="IWO50_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0358223.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IWO50_001088"
     CDS             18181..18399
                     /locus_tag="IWO50_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0358224.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IWO50_001089"
     CDS             18535..19074
                     /locus_tag="IWO50_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0358225.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IWO50_001090"
     CDS             19067..21340
                     /locus_tag="IWO50_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0358226.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IWO50_001091"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IWO50_001091"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0358227.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IWO50_001092"
     CDS             complement(22513..22767)
                     /locus_tag="IWO50_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0358228.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IWO50_001093"
     CDS             complement(23167..23436)
                     /locus_tag="IWO50_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358229.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IWO50_001094"
     CDS             complement(23906..24361)
                     /locus_tag="IWO50_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0358230.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IWO50_001095"
     CDS             complement(24499..26427)
                     /locus_tag="IWO50_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0358231.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IWO50_001096"
     CDS             complement(26624..27457)
                     /locus_tag="IWO50_001096"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0358232.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IWO50_001097"
     CDS             complement(27649..28353)
                     /locus_tag="IWO50_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0358233.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IWO50_001098"
     CDS             complement(28376..29002)
                     /locus_tag="IWO50_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0358234.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IWO50_001099"
     CDS             29328..31703
                     /locus_tag="IWO50_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0358235.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IWO50_001100"
     CDS             complement(31727..32218)
                     /locus_tag="IWO50_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0358236.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IWO50_001101"
     CDS             32405..33325
                     /locus_tag="IWO50_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0358237.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IWO50_001102"
     CDS             33479..33823
                     /locus_tag="IWO50_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0358238.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IWO50_001103"
     CDS             complement(34013..34513)
                     /locus_tag="IWO50_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0358239.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IWO50_001104"
     tRNA            35041..35124
                     /locus_tag="IWO50_001104"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="IWO50_001105"
     CDS             complement(35448..>35882)
                     /locus_tag="IWO50_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0358240.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11869 a         6260 c         7404 g        10349 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcatc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DACEDM010000025        35795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEDM010000025 DACEDM010000000
VERSION     DACEDM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.025"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            214..1272
                     /locus_tag="IWO50_001106"
     CDS             214..1272
                     /locus_tag="IWO50_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0358241.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1291..2310
                     /locus_tag="IWO50_001107"
     CDS             1291..2310
                     /locus_tag="IWO50_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0358242.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2412..3128)
                     /locus_tag="IWO50_001108"
     CDS             complement(2412..3128)
                     /locus_tag="IWO50_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0358243.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(3097..3882)
                     /locus_tag="IWO50_001109"
     CDS             complement(3097..3882)
                     /locus_tag="IWO50_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0358244.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3873..4622)
                     /locus_tag="IWO50_001110"
     CDS             complement(3873..4622)
                     /locus_tag="IWO50_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0358245.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4920..5444
                     /locus_tag="IWO50_001111"
     CDS             4920..5444
                     /locus_tag="IWO50_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358246.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5460..6959
                     /locus_tag="IWO50_001112"
     CDS             5460..6959
                     /locus_tag="IWO50_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0358247.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7047..7649)
                     /locus_tag="IWO50_001113"
     CDS             complement(7047..7649)
                     /locus_tag="IWO50_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0358248.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7753..8709
                     /locus_tag="IWO50_001114"
     CDS             7753..8709
                     /locus_tag="IWO50_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0358249.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(9014..9970)
                     /locus_tag="IWO50_001115"
     CDS             complement(9014..9970)
                     /locus_tag="IWO50_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0358250.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10144..10704
                     /locus_tag="IWO50_001116"
     CDS             10144..10704
                     /locus_tag="IWO50_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358251.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10697..11521
                     /locus_tag="IWO50_001117"
     CDS             10697..11521
                     /locus_tag="IWO50_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0358252.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11531..12148
                     /locus_tag="IWO50_001118"
     CDS             11531..12148
                     /locus_tag="IWO50_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0358253.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12166..12849
                     /locus_tag="IWO50_001119"
     CDS             12166..12849
                     /locus_tag="IWO50_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0358254.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13532..14767
                     /locus_tag="IWO50_001120"
                     /pseudo
     CDS             13532..14767
                     /locus_tag="IWO50_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            14775..15662
                     /locus_tag="IWO50_001121"
     CDS             14775..15662
                     /locus_tag="IWO50_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0358255.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15679..16515
                     /locus_tag="IWO50_001122"
     CDS             15679..16515
                     /locus_tag="IWO50_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0358256.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16518..17141
                     /locus_tag="IWO50_001123"
     CDS             16518..17141
                     /locus_tag="IWO50_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358257.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17138..18430
                     /locus_tag="IWO50_001124"
     CDS             17138..18430
                     /locus_tag="IWO50_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0358258.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18789..18886
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19002..19583
                     /locus_tag="IWO50_001125"
     CDS             19002..19583
                     /locus_tag="IWO50_001125"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0358259.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19586..20893
                     /locus_tag="IWO50_001126"
     CDS             19586..20893
                     /locus_tag="IWO50_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0358260.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21186..21674
                     /gene="purE"
                     /locus_tag="IWO50_001127"
     CDS             21186..21674
                     /gene="purE"
                     /locus_tag="IWO50_001127"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0358261.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21658..22791
                     /gene="purK"
                     /locus_tag="IWO50_001128"
     CDS             21658..22791
                     /gene="purK"
                     /locus_tag="IWO50_001128"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0358262.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22804..24099
                     /gene="purB"
                     /locus_tag="IWO50_001129"
     CDS             22804..24099
                     /gene="purB"
                     /locus_tag="IWO50_001129"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0358263.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24279..24656)
                     /locus_tag="IWO50_001130"
     CDS             complement(24279..24656)
                     /locus_tag="IWO50_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358264.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24912..25640
                     /locus_tag="IWO50_001131"
     CDS             24912..25640
                     /locus_tag="IWO50_001131"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0358265.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25640..25894
                     /gene="purS"
                     /locus_tag="IWO50_001132"
     CDS             25640..25894
                     /gene="purS"
                     /locus_tag="IWO50_001132"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0358266.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25896..26567
                     /gene="purQ"
                     /locus_tag="IWO50_001133"
     CDS             25896..26567
                     /gene="purQ"
                     /locus_tag="IWO50_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0358267.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26568..28790
                     /gene="purL"
                     /locus_tag="IWO50_001134"
     CDS             26568..28790
                     /gene="purL"
                     /locus_tag="IWO50_001134"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0358268.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28775..30214
                     /locus_tag="IWO50_001135"
     CDS             28775..30214
                     /locus_tag="IWO50_001135"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0358269.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30246..31289
                     /locus_tag="IWO50_001136"
     CDS             30246..31289
                     /locus_tag="IWO50_001136"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0358270.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            31286..31864
                     /gene="purN"
                     /locus_tag="IWO50_001137"
     CDS             31286..31864
                     /gene="purN"
                     /locus_tag="IWO50_001137"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0358271.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31861..33396
                     /gene="purH"
                     /locus_tag="IWO50_001138"
     CDS             31861..33396
                     /gene="purH"
                     /locus_tag="IWO50_001138"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0358272.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33412..34686
                     /gene="purD"
                     /locus_tag="IWO50_001139"
     CDS             33412..34686
                     /gene="purD"
                     /locus_tag="IWO50_001139"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0358273.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34808..35722
                     /locus_tag="IWO50_001140"
     CDS             34808..35722
                     /locus_tag="IWO50_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0358274.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT        11421 a         6171 c         7915 g        10288 t
ORIGIN      
        1 ttaagttaac cttaaaacta aacttcttaa gtttggtttt aaggtttttt tgtgttctag
       61 taaaaatcac tttacataag tttcaaaagt ttctttaatt tcctattgtt caataagtaa
      121 caatgtaaag ataaaattaa aaatgttaat acgtattagc aaaataaaag ctttttttat
      181 tgtgaataaa tgaacataat taggaggaga aagatgaact tatttgacat ttttcgatcg
      241 actttgacag atatgtcgat tgtcagtgca atcacttcaa cagtttttat tatattatta
      301 gggtttgttt gtagaaaaaa ggggattttt tctgaatctg ttggaaaaat tctatcgaaa
      361 gtcgtcttaa cagtggcttt gccagcactg gcatttcgag ctttcatgca agatatcaat
      421 acagaattac tgatgcaagg aatcaatgtc ttgatttggg gattagttat ttatattatt
      481 cttattctag tttcaaaacc ttttttctta agctataaag gggataagca aacaacgttg
      541 cgtgtcctga cgatctttgg ttctacgacc tttttcggaa cgcctatcgt cagtgcgatt
      601 tatggtcctg tcggcgtgat gtattcctca attttcaata ttggctaccg aatctttttg
      661 tattcttatg gatacataaa aatgagtggc ttgaagatgg aagctaaaaa tatcaaagcg
      721 atgttcgtca atccaatcat tatcgctact tttgccggcc ttttcatttg gctgttccaa
      781 gggtatctgc cacaagtgtc agttacaggt gcagaaggag agataagcca agtagcgttt
      841 ttgcgaattg accagactgc accgtggcta tttcagccga tggattattt agctggttta
      901 agttcaccat tggcgtggct agcaatcggc gcaacattag gtgaagtaag cttcaaaaaa
      961 gctgcatctg aaaaaacttc atggtattac agtctagtca aagtggtatt tgtacctgca
     1021 ttgaatatcg tgattctagc agttttgacg gcaacgaata ttctaccagt caattttgaa
     1081 gcactggcaa caatcgttat catgatggct acaccagctg caacagtggc agcagcctat
     1141 gctatcagtt ttgataggga ggcactttta gcctccaatg catccttact gtcaactatt
     1201 tttgctgtgg tactgacacc tgtatggatc gttgtattac aaatgattca tggcataggt
     1261 ttattttcat aagaaatgga ggaagaaagt atgagaatcg catgttacgg tgttcgtccg
     1321 aacgagatag atttttttga aaaattgaat atttaccatt atgatctcag tctttatgaa
     1381 gaactgttga cacatgacaa tatcgtgtca gcaaaggacc atgatgctgt attgttgagg
     1441 ggaaattgta tagcagatga gaccaatctt gcaaaaatgc aggaatatgg gattcgttat
     1501 gtttttacac ggactgtggg agtcaatcat atcgatctac aagcggcagc tgattttggt
     1561 atgacagtag cccgtgtacc atcgtattcg ccaaatgcga tagcagagtt atctttgact
     1621 tttgccatga tgcttttgag aaatactgcg tatacgacga tccgaacatc tttcaaagat
     1681 tttcgagtgg atgagcaaat gtttagtcgt gaaatcagaa attgcacagt agggatcata
     1741 ggaacaggca ggatagggtt gacggaagct gttttgttta agggactagg agccaaagtg
     1801 atcggctacg atattcatca gacagaagct gctaaaaaag tattgacgtt ccactcgctt
     1861 gaagagttat tagccaaaag cgatattgtc agtattcatg tacctcatat cccaggtgaa
     1921 aacgatcaaa tgatcaatga aggattttta gctcaaatga aaaaaggtag catcttgatt
     1981 aatacagccc gtggagagct tcaagataat gaagctattt taagagcttt gaaaagcaat
     2041 catttagctg gttttgcaac cgatgttttt gcaaatgaaa cagaggtttt tttccgatcc
     2101 ttcaagccat gggagacgat tccagaccct gctatccagc aattagtaga aatgtaccct
     2161 agagttcttg taaccccgca tgtaggttca aatacagatg aggcattatc aaatatgatt
     2221 tcgacaagtt tcgagaactt tcgtgaaata atagaaactg gaaaaacgaa aaatgaggta
     2281 tctcttccta aagcccgtca attaagataa gaaaacatgg atattcgtca ataaaatgtt
     2341 taaaaatagt ccaagtaaac agttgttact tctgtatact tggactattt tgtatgggat
     2401 aattcgatga ctcaacagta atctgtgagc tttttccgcc cattctttgt caattcataa
     2461 caggttagcg gctggccgat ttccaaatat ttgattcttt cgttcaatat atcttgttct
     2521 actaaaaaat tcaaatattt cttgacggat atacgtgaca gtgagctttt ttgagccagt
     2581 tcttttgttg aaaaaatagt atcttctttt agaataaatg cacttatttt ttttaaagtc
     2641 agttttgaca gacctttagg caaatcgatg aatgatggta tttcgtgaat gggagtggtg
     2701 tggttgaata cttgatcgat caatttctga tcggcttttt cagtcgtttg gattgtctga
     2761 gagaaagcaa gatacttttc gatcgccagc tgaaaacgtt gcaaagtgaa cggtttgatc
     2821 aaataatcta aggcaccgta gcggatcaca ttctttatgg tttcgatatc atctgcagca
     2881 gtgatcaaaa tcactgggat atcatttcct tgctgtctaa gccaagataa aaaatggata
     2941 ccactttcgc caggcatgta tacatcaagc agcagcagat ctgcgttttt ttgtctgaaa
     3001 tatttttttc cttcttctac atttctgaaa ctttcgatta cgttcatatt agggatgctt
     3061 tccacaaaag tacgattcaa ttctgcaacc attggatcat cttcgattat caggacattt
     3121 gtcatcattt tgatcttcct tttttggtaa ttctagatag acgattgtac cgccatttgt
     3181 ttgtttggaa aattctaaaa atccaccgtg gcgtttgacg attgaatgga tgagattcaa
     3241 tccaaaccca cgattgattc ccttcgtcga aaaacctagc gtaaatattt tttctgagat
     3301 ttcttctgat atcccgacac cgccatcgca gacggtcacg atgaatactt cagcttctgt
     3361 gtcatagttt aaatgaatcc ggacctgtcc gtttctttca tgtttttgta gcacggcttc
     3421 ttttgcatta tcaagtaagt tccctaacac ttgcaaaagt gaatggatgg agtcatccat
     3481 ctccatttta gggataaaag aatcgctttc taacagcatt tcaacagatt gttctttggc
     3541 ttcgtttatt ttgcctaata aaaaaccggc aattgcaggt actttgatca gacttgagac
     3601 ttggccaatc tcttttttat aattttgatt tagctgctgg ataaaatggg tgacttcttc
     3661 atacttcttc aaatcaatca aaccagaaat gacatgaagt ttattcatga attcatgggt
     3721 ttgagctctc atggagttta tatattgttt cgtcccccca agctcctcca ttaatttcat
     3781 tacttctgat cgatttcgta aagttgcgac tgctcctaaa aaacggtcat tgctaaaaag
     3841 aggtgtaacg ctagaaatag tttctatccc atttaaatac atggattgag tccgaacggt
     3901 cgtttgatgc tcaatcgtct ctttaaacaa aacgtcatat agattatcac tgattttttt
     3961 cctacttcga aatccaatag ctgctctgtt tggtagaaaa tgttttttgc aggtgtattg
     4021 atcaagagga tagttttatc agaagagatg gctaagattc cttcatctac ttcattttcg
     4081 ataatcgttt tttcacttaa ctgttctgct atttcttgtg gttcaagtcc aagcagtact
     4141 tttttcattt tttgggcaag aaaaacggcg cctatgatgc ctacgctaag tccaataatc
     4201 aatgaaaaaa caatcatttt ttgtccatct ttgacttgat tgtcaatcgt gttgtttgtt
     4261 aagccaacac aaactgcacc aataatctct tgatcataat aaatagggac gaaatagcgg
     4321 aagccgaacc ctaacattcc ttctttttca gagaaaaatt cttgtcctcc aatcactttt
     4381 ttcacatctt catgacttga gaaaggaaga ccgatgtaac ttgggtctgc gtgagaatat
     4441 cgaatgcctt cagggtccat gacaacgata aaatctaaat tcgatgcaat catgatttca
     4501 tttgttattc cttgaatcgc atgtgtcggg tcaccagtct gcagcttttt ttggatgagt
     4561 ttatcattgg caacgatttt agcaatgcta gagattttat ctgacacact agatcggttc
     4621 atcgaaagaa taaaattcct gatcaatatg gcagaaacaa tcaaagaaac gaatgtggag
     4681 aaacaaacga caaggatgat cgtgttctgg agctttaatg gttttttctt aaaaaaagga
     4741 aacataggct acctcgaaag aatttattgt gaaaatttta acatgaaaac aaaaatttaa
     4801 caaattgtca acgataaaca caaccttcct ataaaaaaca ccactgagtc ggtaaaagaa
     4861 gctatacaga attttcaaac attggtatga tagacgtaga aatggaagga ggcgtgtata
     4921 tgataggggg aatcgcactt gagacgattt attggtatac attgctgatt tgtgcaggat
     4981 tggcagtcct attgattatt tttggagatg tattcgattt tgatggcccg cttgatccga
     5041 tgcttattat cccttggtta actttcacaa gcttatttgg ttttctaggt gagcgtttaa
     5101 cagatcaatc atctttattt ttatttattg ctagtggagg catatcgata gtgttggtat
     5161 ttttgcttaa tttctatata ttgatgccga tgaaacatgc cgaatcaacg atgtcgtctt
     5221 ctgaaaaaac tctggaagga caagtagctg ttgtgatcac accgatccct gtacgaggta
     5281 tgggagaaat tcagttgaaa agtgttaccg gttccattag tcgtccagct tgtttttaca
     5341 gcccccaaga acagacagct gagcggggaa cgaaagtatt gatcattgag atcaaggaaa
     5401 gaatctgtta tgtcatgcct tatgaaagca tgataaaagc ttaaaggagc gtgtgttaaa
     5461 tggaaatacc agttgttttt atcgcgatcg caatcgcagc tttgatttta ctgatgttgc
     5521 tgatcgtctt tgtagcgaag taccagacag caaaaccgga tgaagcgttg atcatcagtg
     5581 gaagttattt aggaagcaaa aatgtgcaca ttgatgaaag caataacaaa atcaaaattg
     5641 tgcgtggagg tggcgcattc gtcttgccag tttttcaacg ttcgaatcgg atcagtctat
     5701 tatcgagtaa attggacgtc tccacccctg aagtgtatac ggaacaaggc gtaccggtga
     5761 tgtgtgatgg tacttcgatc atcaaaatcg gttcatctgt agaagagatt gctacagcag
     5821 cagaacagtt tttaggcaag acaagagaag aactggaaaa tgaagcaaga gaagtattag
     5881 aaggacactt gcgctcgatt cttggttcta tgactgtaga agaaatctat caaaatcgcg
     5941 ataagttcag tcaaagcgta caagaagtcg cttctgtaga ccttgcaaaa atggggttga
     6001 tcatcgtttc tttcactatc aaagaggtca gagacaaaaa cggttatctt gactctttag
     6061 ggaaaccaag aatcgcccaa gtcaaaagag atgcagatat agcagaagcg gaagctctaa
     6121 aagaaacgcg tatcaaaaaa gctcaagcag aaaaagaatc acaagcagct gaattacagc
     6181 gacaaacaga gatcgcagaa tcattaaaag aaaaagaact gaaattagca acctacaaac
     6241 aagaacaaga tgttgctaaa gcaaaagcag accaagccta caatttagaa agtgcccgag
     6301 cacaacaaca agtaatcgaa caggaaatgc agattaaaat cgtagaacgc caaaaacaaa
     6361 tcgaactgga agaaaaagaa atcacacgtc gtgaaaagca atatgattcg gaagtgaaga
     6421 aaaaagcaga tgccgatcgg tacgctaaag aacaagaagc acaagctcaa aaagtcaaag
     6481 aagtgactga agccgaagca gaacgattcc gtgtggaagc actggcagaa gcggaagcca
     6541 ataagacacg tttagctgga caagcagaag ccgaagcagc actagcaaaa ggaaaagcag
     6601 aagccgaagc aaaacaaaag atcgccaatg ccttcaagga atatggcgaa gcagctgtct
     6661 tgagtatggt tatcgatatg ctaccgcaat tgatgcgcga agcagcacag ccgctaggaa
     6721 atatcgaaaa aatctctgtt gtcgatacag gaagcagttc aggagaaacc agtggagcga
     6781 accgagtgac aaattatgcc acaaatttgc tttcttctac tcaagaaaca ctaaaagcga
     6841 caacaggact tgacttgaag gaaatcgtag agaatttgtc gacacgtggg actactagcc
     6901 aagtaaatat gtatgaagcg ccaaaagagg cagaacaaaa ctcgatgaag gaagaataaa
     6961 gtagtacact tcaccgttca atcaaaaatg gaggaagact ttttaaagtt cttctctcca
     7021 tttttttatt ttattgcaaa agaaaattag tcctcgctat ttttcttttg tttttggatc
     7081 ttagcttcta tctcttgtaa atttgttctt agcaatcgtt ctttcttttg gagttcgacc
     7141 aaatcttctg atagttcttc cttcttttct gtttcaggag gacgaagaaa gaaattgata
     7201 ccgattgcaa tgaatgtccc tataaaagta tctagtacac gttggatagc atacaaagcg
     7261 gattcgcctt gagggatgct caacgcaatc agcaacaacg tagctattgc ggaaatgata
     7321 ccggaattat tgttaatacc atctgacacg acgatcacaa taataactag aataggaagc
     7381 aagatcaatt ctaccaaaaa atcattgtga aaatagcttt tgacaaaaaa ataaatgata
     7441 gctaatgtac cacctaatgt attgccgatg atcctagact ttccaaaaga aacggttgta
     7501 gtcaagtcct gacgcaaaga aaaaacagca gataatgcag caatcatcgg gattcctcgt
     7561 tgagtcaaat gaaaaaacaa gatacataaa aaaacagcta aggcactttt gatcgtacgc
     7621 atacctagtc gaaaacggcc aaattgcata gagattctcc tttttggtct ttttactatc
     7681 atacctaaac ttttgtctga tagctagttt ttcattattc aagtataatt aaaataaaaa
     7741 agggggaagt aaatgcgtat aggaatgcga acagtcaaaa cagtgatcag tgtcttcttt
     7801 tcgatggtca cagcgggtag cttatcgctg ttgtattggc cagctgcagg gatcattgca
     7861 ttattaagtg taggaaatac caagcgctct acattgcgta cgggcatgaa tcgaatcgca
     7921 gcctttatat tagctacggc aacagcactg ttcagttttt ttgctgttgg gtatactatt
     7981 tttggcttca gtcttttttt attcctgttc atcccgcttg cctctcgctt caaattaacc
     8041 gaagggatag tagtaaattc ggttttggtc actcattatt tagtggagga aaacatgtct
     8101 tggcagctga tcggcaatga agcgggatta atggcgattg gattagtatt tgctttgctt
     8161 gcaaatgttt acatgccaga tcgtacgaag cagctgaagg aaaaccaaat acagatcgaa
     8221 aaagaatttc gaaatatttt gagacaaatg gcagaatttc ttttatcaga aaataaagaa
     8281 gatgtgcaga tcaaatgcga acatttactg acattcattc gcgaaagcca agaagatgcc
     8341 agagaacatc aagaaaacta ctggctcaga cagccgctat actacgaaac ttacttctct
     8401 atgagaagag cacaggccaa tgtgattaaa gacatgctgg aaaatctaga gagaatccag
     8461 caaccagctt attatggaaa acatatctat ggtttattga tttatactgc agaaacattt
     8521 tcagagtcaa atgatggcag acaaatttta acacgtattg aagaagttta tgtattgtat
     8581 cggcaaatgc ctctgccgac tagtcgccct gaattcgagg atcgagcaga attgttccag
     8641 tttttgcagt cttttaaaag ttttattgaa atcaaggcag aattctccca acaaaaaatc
     8701 caaaaataga ggttgtgaat tcagctaata ttcaggtcaa aaagctgatt tcacaacctc
     8761 ttctttaaac ggtgttctcc tgtcaactcc tcggtcacaa gtcacaatat tcaacaaaca
     8821 tgagaagctg ttttttgaaa ttgctccttg tgactcggag ctgaacggta ggctcacaac
     8881 ctctttctta aacggtgttc aaaagctttc ctctgcggta ataagtccag ccaaacgaaa
     8941 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     9001 caacctttta aaattacata tttttccata gaacttgcat ttgatccaag atttcgatga
     9061 catcttttgt caagaagaga gagcgattgg gcaagtcacc ttcaatcatg tgtaccatat
     9121 tctttatttc gtaattcatt gcttcagatg tattcccgct ttcgatgaat tcctttgtac
     9181 catctgtaaa taagatctcc gcacgatcag cacgcgggta atcagcaatc gtcacataac
     9241 cgttctcaaa ggcaacgata ccgacttttg gcatttttgc ctgaaaagtc aagctgacag
     9301 cagcaagttc attttctttg ttacgcaaga tagtgacgga ttgttcgtcc acgcctgttt
     9361 caaaaggaac cattgtagag gcaactactt caggttgaga gctcaaaaag aaacgagcga
     9421 aagatacagc atacgtaccg atatccaaca gagctcctcc agctaattct ggattgaaga
     9481 accggttttt agggtctggt tctttgtaac tgccgaaagg tgcttggatc atttttaaag
     9541 cccccagttt tcctgtatcc attattgagc gtaactgctg ataaagtggc atgttgaaga
     9601 ttgtcatcgc ttctgctaag atcaaattct tttcttctgc taagcgcata gcgtcagcca
     9661 gttctttttt attcatcgtg atggcttttt cacatagaac gtgtttgcct gcttccaatg
     9721 ctgccaagat atggtcgata tgctgtctat ttggtacagc aatgtacacg atgtcgattt
     9781 cagcatcgtc taacatttct tgatagcttc cgtaagcttt tggaatattg taacggtgag
     9841 cgaagttttc cgctttttct gaagttctgg aagcaactgc tgttagttcg ctcgtctctt
     9901 gattgaagct ttcggcaaac tcatgtgcga tggtaccaag accaacgatt ccccattgat
     9961 attttttcat ctgaaaacgc tccttcatag ttggattgag tttcttgtct gtctattatt
    10021 ataacaaaaa tcacgaagaa taaagaggag aggacttgaa acattaaaaa aaatgaaagc
    10081 agcgatttaa gaggtgcttt ttgatcaaaa cttaagtaga atagttaaaa ggaggggata
    10141 aacgtgcatc aagtatatat tgatcttatt atgagtgtat tattagaaca gtttgaaact
    10201 gaaaaaaatt ttttagatga atacttgctg atcagtccag aacaatggca aaaatggaaa
    10261 gatggcgaga aaaatctgcc atctgaagcg atgcagaaag taaaaaatct tttcactgat
    10321 tatgaatgga tgctaacgca aaagattttg cgtcaaacga ttatttttcc cgaaaaacga
    10381 aacgttgccg ttgcagagta caagcaaatc aaaacacgca tcgcgcaaaa atggcttagc
    10441 agcaaagcag caaaagtcga gctgatccct ttgaaaaata atcagcaggc tggtagatac
    10501 cttgacttga aagtaagtat tcaatatgat gaatggggct atgatgatat tttgaatttt
    10561 cgtctgccag cagatgttca aaaacagatc gaaggagaaa aaatcgaact tctcgattgg
    10621 gtgaatgaaa atctggaaga aacttacgtt catgaggcga atggggaacg tgaagaacaa
    10681 cgaaaggatt tttcaagtga ataaattttt aggatttgga gcagttgtga ttatgatcgc
    10741 agcaatgggc tatgttattt ttagcgatga tgattcgcat gttcaagccg gcaatcaagc
    10801 agtcgaacga acaagcacca aaacaactgc tagtaaagaa aagaacagtc aaacaaccag
    10861 cagcaaggta aaggacaaag aaacaaaaga tcgtacgtcc aaacaattgc cagatgttaa
    10921 aacagacgat tggaagctag ttttggttgg tccagatcac aaattagaaa aagaaattga
    10981 tgagtctact caattggcat cattaagtaa cggatacttg atcgacaaac gcattgctcc
    11041 agaatatgaa gcatttgcca aggctgctga agcagctgga tttccattag tgatggtctc
    11101 agcttaccgg tctgtatcat cccaacaaca agttttcgaa caaaatgtcc aagatgtgat
    11161 gagtcgtcaa ggtgtatcag aagaggaggc gacaaagatt actaaagaaa cgattacgga
    11221 gccaggatac agcgaacacc acacaggtct ggcagtcgat gtcgtcgatc aaaactggta
    11281 caacagttat ccttctactg tattggatgc aagttacgga gatcagcctg gagctaaatg
    11341 gatcgcagat aatgctgcgc agtatggatt tatcgttcgt tatccaaaag gacacgaaga
    11401 tataactaag atcacatatg aaccttggca cttacgttat gtcggtaaag aaaacgctga
    11461 gtatatcacc aaacaccaat tgacgctaga agaatatctg aatgaattaa atgaaaagta
    11521 ggaattgatc atggcgaaaa aaaagtggaa aaagcaaaag cgcaagaata tccgttggcc
    11581 agcagtggtt gccggtgtag cagtgatact cttagccttt gttttttcct tgaagaatct
    11641 gtcttctcct tttactgata atcagcaaga aaatgaagcg caatcccatc agcaatttat
    11701 tgaacggctt gttccgcatg cccaagaact acaagatggt tatggagtgc ttcctagtat
    11761 cattttagga caagctatat tagaatcaaa ttggggaaaa agtaccttgg ctagcaaata
    11821 caataatctt ttcgggatca aggcttatgg ggatcagaag aaagtaagcc tggaaacaaa
    11881 ggaatttgtt aacgaagaat ggataactat ccaaggagac ttcaaagtgt atgattcatg
    11941 ggaacagtcc atggatgacc atacacaact gttcgtccaa ggagtagatt ggaatcctgc
    12001 gctgtatgaa aaagtgatta cggccaccaa ctatcaggaa gccgcacaag cattgcaaga
    12061 tgcaggttat gcgacagacc ccggttatgc gcaaaaaatc atccaggtca tcgagacgta
    12121 tcaattgaat cagtatgacc ggagatagca aaggagaagt ggctgatggc agaacaattt
    12181 acgccagaaa tcatgacgga attgagaaaa gatatcgtaa gagtaccgga aatcatttta
    12241 gaagcaagcg ggatcaagat atttggaaaa ttgatccgtt cgatcatctt tactacggat
    12301 attgcgatca tacgtaatac aaatgcgaat gcagtaattg ctgtgtatcc ttttacacct
    12361 catccagcga ttactaaaag cattattgag gcagctgaca ttccagtttt ctccggtgta
    12421 ggtggtggtt tgacacaagg tgctcgttct tcctacatga gcatgttcgc agaagcgcaa
    12481 ggttcgttag gggtcgtttt gaacggtcct acgcctttag agacagtaaa agaagtttgt
    12541 cgagtagttg atattccagt tatcagtacg gtaacgtcga aatatacaga aatagatgaa
    12601 aagttagcag ctggtatttc gattatcaat atcagtgcag gtaaagaaac tgcagaagca
    12661 gtacgccatt ttcgtaaaaa atatcctgaa cttcctatca ttgcgacagg tggtccaacg
    12721 gaagaaagta ttgctgagac cattcaagca ggagcaaatg ccatcacata tacgccacca
    12781 agtagcggag agctttttag tcgtaaaatg gaaaaatatc gggaaactga gaaaaagaat
    12841 catgaataac aggtgagaat caaaagacga ggaagagcag atagtggctc ttcctcgtct
    12901 tttgtactcc agtactattt gttcagaatt caaaaaaaag agaaattttt ttgttttttt
    12961 acctgtaatg tacataagaa gagttaaaac cttttgttat tattagcatt ctaacaaaaa
    13021 aatgtcttga tttattgaag cgctttctat agaatgaaaa acgaataaaa actaaaaaag
    13081 gcacccgaca atttagtagc agctaaattg tcggggcttg aaaagtcagc gggaactgac
    13141 caatcaagtt gccatagtca atgcttgtgc atcgacgtgt atattgtacc taatttcata
    13201 cagaattact agttattttt ctgtttttta gggaagagcg cacacgaaga agcatgctta
    13261 tttagctatg tcaaggatat tgggcagttg tctgatatcc ttttttcttt gcgtaattat
    13321 cctcccatca ttgaagcaag aagtgttttt agcgatgaaa gaagaatcag cactatctaa
    13381 tttttcttga aagtattgag ttgttcatcc taggcagaac gataaggcaa gtcttggttt
    13441 ttattcccct tgaaaaagat cgtttaataa atatgttctg tataaaaata cagtatattg
    13501 aaaaataaca atggaagtag aggtgcagag aatgaagaaa agaatgagaa tcatcttagc
    13561 tttaagtgtt atgttattct tcttgacggc ttgtgggcaa gaagatgaca aaaaaatcac
    13621 gctgaacatg gcttggtggg gttcgcaagt ccgacacgat gcaacagtaa aagtaatcga
    13681 gctttatgaa gaaaaaaatc cccatgtgac gatcgattat gaattttatg attatgaagg
    13741 atattacaca aaattaggta cactagcagc atcagatgat atctgggaca ttttccaact
    13801 gaacaatcaa tttcctcaat acatcaatca gattgaacca ttagatgaat atgtggagca
    13861 agggataatt gatatttcta atgcagatga atcctattta gcaacgacga cttacgacgg
    13921 gcaattagtt ggactgtcta acggggtgaa ttcgtttgcg attgcttaca ataaaaatat
    13981 atttgaacaa ttaaatatcc ctgaaccagc ttcaaattgg acatgggatg aatttgaaca
    14041 aatcagctat caaatcactg aagaattagg aatctatgct atttcaagat tcgaagattt
    14101 tatagctggc tgtattattc aaataccaca agttgagaaa ggattgaatt tttataatcg
    14161 ggaagatcta tctaacagct tgggatttga aaatccagac tatctaacag attttttcca
    14221 gctgcgtaaa aatctggtag acaaaggggc ccatccagaa ccaggtggtg gagctgcaag
    14281 tacgacggat ctgtcttttc aggcggttag ggatagagaa gcggcaatgg tgtttctatc
    14341 aagtaaccag ttgacagaaa tattgtcagg ggctcctgaa gatatggaga ttcagttgat
    14401 taatccacct cgcagaaaag cagatggaga atctggtatc cctttacgtt cttcgcaaat
    14461 gctgagtatg gcaaaaagct cagcccataa agaagaagct gctaagttta ttgatttctt
    14521 tcaaaacagt gaagaagcca atgaaatttt aaaaggagaa cgtggtgtac caatcatgtc
    14581 gaatattcga gaacagctcg ctcgtgcaga tgatgcaact ttgcaagcga catttgatta
    14641 tttagaaatg atttccgata tggacaacgg cgaagtgaat gtctttgata gtgctttgaa
    14701 tccggaaatt caagaccaat acaatctttt agttgaaaaa gtcatttatg aagaagtaac
    14761 agcagaagga aggcatgaaa aaagaaaaac taaaacggtt cttggatcaa gacaatgtgg
    14821 tcggttacgt atttgcttct ccattcatcc taggatttct attgataaca gtgataccga
    14881 tgatcttgtc actttattat tcgttcaccg attatcgttt gggaaatcca gtctcatgga
    14941 tcggcttgga taattatata aagatgctga gtgatgcccg ttttttgaac tccatccgag
    15001 tcacttttct ctatgtattt gcatctgtac ctgtgaaact gatctttggt ctattgattg
    15061 cttatctttt aacgaggaat atcaaaggaa cgactttctt tcgttctttg tattatgtgc
    15121 ctagtttgat cggtggcagt atttctatcg ctctagtttg gcgggagttg ttttctaaaa
    15181 acgggttagt gaatattttc ctggaacgat taggcgcacc tgcgctttcc tggtttagcg
    15241 atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc ttggcaattc ggttcggcaa
    15301 tgatcatctt tgctgcaggt atcaaagaga tcccaggctc gtattatgaa gcggcagaga
    15361 tcgatggagc gaacaaatgg cagacgtttt acaagatcac gttacctctt ctttcgccga
    15421 tcattttgta taacttggtt atgcagacaa tcgcggcgtt tatgactttt acgcaggcat
    15481 ttatcattac ccaaggtggt ccgaatgatg ccacgaactt ttatgctctt tatgtgtata
    15541 accaagcttt ttcttttaat tctatggggt atgctagtgc tatttcgtgg atcatgctgt
    15601 tgattatggg agctgtcacc tttctgattt tcaaatcatc aaaaaaatgg gtcgttgtgt
    15661 gaatgagagg aggaagaaat gaataataca gtaacgaaat ggtttcaata cggttttatt
    15721 ttgctattag gtctagtcat gatttatccg gtcttatgga tgatcgcagg ttcttttaaa
    15781 acggatcaag agattatgag tggttcgctg aatttgattc cagagacttt tcaatgggaa
    15841 aattacgcaa aaggatgggc ggggtttgct ggaatttcat tttttacttt ctttaggaat
    15901 tcgtttgtca tctctgcagt ttcgacgata gggacagttc tgagctcgac ttgtattgct
    15961 tacgcattat cgagaatcga tttcaaagga aaaagattct ggttcgtcgt catgttagta
    16021 acgatgatga ttcctgcaca agtgattttg attccgcagt ttattattta caatcggctg
    16081 aatttggttg ggacgtatgt gccgctgatt ttaccacatt tttttggaca agcatttttc
    16141 atttaccaga tcatgcaatt catggtcagc atccccaaag agttagatga atcagcgatt
    16201 attgacggat gcagcaagta tagtgtcttt accaaaatta tttttccttt gctgaaacct
    16261 tcgatcatca cgacgatcat catccagttt tattggaaat gggacgactt catgggacca
    16321 ttgatttatt taaacaagcc gagatcttac acggtatcga ttgccatcaa attatttgca
    16381 gacgctggat ctagtacgac ttacgcgagt atgtttgcga tgtctacgtt gtcgctgcta
    16441 ccggtttttc tgattttctt attcttcaat aaatatcttg tccaggggat cagtacaagc
    16501 ggtctgaagg gctgaatatg aaaaagccaa tagaagagat gtttctttac aagctcaact
    16561 actatgtctg tcggattttt ttaaccagtc ttatcagtaa tgctcttttc gctttagttc
    16621 taggatgttt gttcacttta ggattagtgc tgtctgctcg tgttgtctat ctgatttatt
    16681 ttttgatgat gtatccttct tattatctat ctgtccatta cttggcagaa aaaaaggtat
    16741 gttggaggaa agggaaaatc aaacggatgt gtttcatcat tacgttgttt cagctgtttg
    16801 tcattatttt tgcagttgat tggtatttat tagtgggaaa gattggagct tggtatctga
    16861 ttccttttct attgtgtctg ttgctttttg tatcccatag tttttttagt acgctaattg
    16921 atatctgcaa agaagataaa gcagatagtc ttgtttattt gatcaaaagg aatatctggc
    16981 tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg ccttttgtat ggactgttct
    17041 ggtgtataca gcctgatttt ttactcactg cattactgta tccagggctg ccatttgtca
    17101 cagtattttg cttaaaagaa aaggagaatg actttaaatg aagaaaaaat atgtacaagt
    17161 cggacttggt gggagagcgc gtttttttta tgaggcaatt gcctctacgt atcatcaaac
    17221 ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg atggattatg caaatcggat
    17281 acttgtgaac aagtatggac agaaaaaagt aaagacatgt atgccatcac aatttgagca
    17341 actactggat gacgtacggc cggctgaagt cattattacg tcagtagatc gaacgcatca
    17401 tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt atttgcgaaa aacctctgac
    17461 tatcgatgag gaaaaagctc aagaaataat aacggtccaa aaacagacgg gaagaagcct
    17521 gcgagtcact tttaattatc gttatgcacc gtatcacacg aagataaagg aactgataga
    17581 aagtgatgtg attggtgatg tgttttctat ccacttcgaa tggctgttaa atacgcaaca
    17641 cggggcagat tatttcagaa gatggcatcg aaataagcac aatagcggcg ggcttcttgt
    17701 tcataaatct acccatcatt ttgatctagt gaatttttgg ttgaacagcc gtccccaaaa
    17761 ggtatttgca ttcggtggat taaactttta tgggatgcat aatgcggaga aacgcggagt
    17821 agaaatattt tacagtcgat gctatggaag cgaactagct aaagctgatc catttgctat
    17881 agacctgtca gccaatgagg aattgcgaga aatgtattta gaagcagaaa aagacagtgg
    17941 atatattcgt gaccgcagtg tattttccga tgatatctct attgaagata cactaggcgt
    18001 aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg ctaaacgcgt atatgccgtg
    18061 ggaaggattc aatgtttctt tcaatggcag caaaggacgg atcgaaatga aagtgatcga
    18121 aaagtcttac atcaatgcag gtggagataa aaaggatgaa ggagcagccg ttcttcagtc
    18181 catccatgtc tatccgatgt tcggtgagcc atatgaggta atcgtcgaac aagaagcggg
    18241 cggacatggc ggaggagatc cgcgtttatt agatgatcta tttggtgaag aaaaagaaga
    18301 agatccgtgg aatcgggcag caactcacgt agatggtatt ttgtcgattt tgacaggaat
    18361 cgcagcaaat cattctattg cttcaggaaa agcagtagtg attgacgagc ttgtcagctt
    18421 aaaagaataa gaatagattt acggaaatta cttcccgtag tttataaaat cttgccttat
    18481 tgccgatagg ctgtcttttg aacactattt attcggaaaa aggggctatg acatgctttt
    18541 tgtcatgcct tctttttatg tgggctgctt ttttagatag gagaaaataa aaataactat
    18601 ttaaacccga acaaaaagga ataatgcatt aaaaatcatt agattttaca aaaaaataag
    18661 taaaccacgt tgtcagtttc agaaagtagt gttagactgt tttcaatatt tgaaacaaca
    18721 ttttttattc aaatatggca aactgccttc acgtgcaaaa cagttgcaag aaaatgaatt
    18781 tacactatta ttagttttac atacctactt atatatcgtc ataatatgga tgacagtttc
    18841 tagccagtac cgtaaatgct ggactataag taaaagattg gctatttcac ccttcttttc
    18901 ttatgataaa gtggagggtg tttttcttta acagaaacat agtaggtttt tgcacaaaag
    18961 cggttatttc ggtattaata aatgataaag gagcaaataa agtgaaagaa ttagttgaac
    19021 gtattcaaaa agacggacgt atattaggcg agggcgtatt aaaagtcgac agcttcgtca
    19081 ctcaccaagt agaccctgta ttgatggaac aaatgggaaa acggtttgca gaagtattta
    19141 aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc tgggatcgca ccagctttat
    19201 ttgctgcaag agaattagat gttccaatga tcttcgctag aaaagcaaaa agtttgacga
    19261 tggatgaaga attattgaca gcttctgttt attcttttac gaaacaagtg acaagtacga
    19321 tttctatttc tcgtaaattt ttatcttcag aagacaaagt attgattatc gacgatttct
    19381 tggcaaacgg acaagcggca aaaggattga tcgaattatg ccagcaagct ggagcgcaag
    19441 tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga tggacgtcag ttacttgaag
    19501 aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc cttgacgaat ggacaagttg
    19561 agtttcttga ggaggacgca taatcgtgga aaaagaaaca caaaatggaa aagctgcggt
    19621 tttagggctg cagcatctgc tagcgatgta tgcaggagca gtagcggtgc ctcttttgat
    19681 cggtacagga ctaggcttta atcaagaaca gatgacttat ttgatttcaa ttgatatctt
    19741 tatgtgtgga ttagcgacat tattgcaatt gacagttaac cgatttttcg gaattggctt
    19801 gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca ctgatcttga tcggaaccaa
    19861 tgaaggagta ggagctatct acggatctat tatcgcttct gggatctttg ttgtgttagt
    19921 ttctggtgtc ttttctaaaa ttaaaaaact attccctcct atagtaacag gaacggttat
    19981 caccgttatt ggtttgactt tgattcctgt agcaatcgag aaaatgggtg gaggtaatgc
    20041 aacagcagct gattttggtg acaaaacaaa tctgctactt gcatttgtga cgattctcct
    20101 aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt tcaatcgcgg tattgattgg
    20161 tttagtcgga ggaagcatct tagctgcttt catgggaatg attgatctga gcgcgattgg
    20221 agatgcgccg ttgttccatg ttccacaacc cttttatttt ggtaaaccaa ctttcgacgt
    20281 atggtcgatt ttactaatga tcatcatttc catggtcagc atggtagaat caacaggtgt
    20341 atattttgca cttggtgaca ttactgggaa aaatgttgga gaagaagaat tgaaaaaggg
    20401 atatcgtgca gaaggacttg ccgttatctt aggcggtatt ttcaatacat tcccttatac
    20461 tggcttttct caaaatgtcg gattggttca actttcggga atcaaaacaa gaaaacccat
    20521 ctatttctct gcattcttct tgattgtttt aggattattc cctaaaatcg gtgcgttagc
    20581 acaaatcatc cctgagccgg tacttggtgg aggaatgtta gtcatgttcg ggatggtggc
    20641 agtccaagga atccgtatgc tgttggaagt tgatttcagc aatgataaaa atctattgat
    20701 tgttgctgtt tctgttggat ttgggctggg tttcaatgtt acgccgacat tattcagtca
    20761 aatgccgcaa acattgcaaa tgttcactgg aaatggcatc gtaatgagta gcattacggc
    20821 tatcatattg aatttgatct ttaatggatt gaaaaaagat gaagaaatat tggaagaagc
    20881 cttcaagaag taatagagca aaagaaaggt tgtgaaatca gcgttttgac ctgattgtta
    20941 gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt tgtttgagct aataccgagg
    21001 gtcagctgat tgaacaccgt ttatccataa aaaggaatgt gacctaactt ttgtcacatt
    21061 cctttttttc atctttaata aacacgaaca ataaaatgtt tttctttgtt aacattcgaa
    21121 atttctttga catcaaaaaa agtgattgtt aaaatatgaa tgtagaaaaa ctgggaggaa
    21181 aaacaatgac agcaaaaatc tctgtgatta tggggagtac ttcagattgg gaaacaatga
    21241 aagaagcatg tcaaatattg gatgaattcg gtgtgtctta tgaaaaaaag gtcgtttctg
    21301 ctcatcgaac cccagatttg atgtttgaat atgcacaaaa cgctcgaaaa aaaggaatca
    21361 aaatcattat tgcaggagca gggggagccg cgcatctgcc gggtatggtc gcatcaaaaa
    21421 caactttgcc ggttatcggt gtaccggtcc aatcacgaac attaaatggt ttggattcgc
    21481 ttctttcgat tgtccaaatg cctggaggag tacctgtagc tactacagca atcggaaaag
    21541 cgggagcaat taatgctgga ctacttgcga tacagatgct ttccatgtat gatttagaat
    21601 tggaggctaa attggctgaa agacgttcaa tgttggctaa aactgttata gaaagtagtg
    21661 atcaacttgg ttaaaccttt gttgcctggt tcaacgatcg ggatcgtcgg tggcggtcaa
    21721 ttaggtcgta tgatggcgat tagtgcaaaa gaaatgggct ttaaagtagg tgtgctggat
    21781 cctgtgagcg attgtccagc cgcacaagtt gcagattggc acattgaaag tgtctatgat
    21841 gacacctttg cgctgaaaga gcttgccaga agaacagatg tcattaccta tgaattcgaa
    21901 aacgtcagtg tagaggcgtt aaatgcgatt ttacccatgt catttattcc tcaagggaca
    21961 gacttgcttg cgattacgca agaccgcctg ttagaaaaat cttttttgga aacgaataac
    22021 atcgtgattg ctccttatgc aacgatcgtc agtccgacgg atatccaaga tgcgattgac
    22081 gggatcggct atccttgtgt tttgaaaacc actcgaggcg gctatgatgg caaggggcag
    22141 tatgttttga aaagtcgggc ggatctagct cctgccatgg accttttaag agaaggaacc
    22201 tgtgttttag aagcatggat cccgtttgaa aaagagattt cgatcatggt ggcaggaaat
    22261 ggtcaaggag atttcacgac ttttccggtc gtggaaaaca tccaccacaa caatattctt
    22321 catgaaacga ttgctccagc cgcgattgat caggatgtga tcgaggaggc tgagcgtatt
    22381 gcccgtgtga ttgcagaagc agtgagcctt tcaggtgttt taggggtaga aatgttttta
    22441 acaaaaacag gcgggctata tgtgaatgaa ttagctcctc gtcctcacaa ttctggacat
    22501 tattctattg aagcttgttc tatgagtcag ttcgatgccc atattcgagg tatttgtggt
    22561 tggccattag gagaaaaagt acgtttgata agcaatgcag taatggtcaa tgttttggga
    22621 gaacaactgc tggaaagcta tcgcttgatt gagaataagc cagactggca attccatttc
    22681 tatggaaaaa aacaagcaaa aaaaggtcgc aagatgggac atatcactat cttgacagaa
    22741 gacatccagc aaacgctgga agaaatcaaa caaacgaata tttgggatta gaggagaaaa
    22801 aacatgttag aacgttatac acgccctgaa atggggaata tctggacgga agagaacaaa
    22861 taccgtgcgt ggctcgaagt cgagatttta gcagatgaag cttgggcaga acttggtgaa
    22921 atcccgcaag aagatgtgaa aaaaattcgt gagcatgcat catttgatat cgatcggatc
    22981 ttggaaatcg aaaaattaac acgccatgat gtcgttgcgt ttacacgagc ggtttctgaa
    23041 acattgggag aagaaagaaa atgggtccat tatggtctaa caagtacgga tgtcgtagat
    23101 actgcttatg gctatctcat caagcaagca aatgatattt tacgggaaga cttgaaacgt
    23161 tttacggaaa tcattggcga aaaagcaaaa gaacacaaat atacagtgat gatgggtcgg
    23221 acacacggtg tccatgctga accaactact tttggattga aacttgccac atggtattca
    23281 gaaatgaaac ggaacatcga acgatttgaa catgcggcaa aaggagtaga agcaggaaaa
    23341 atctcaggag cagtgggaac gttcgcaaat atcccgccat ttattgaaga gtatgtatgt
    23401 aaacaattgg ggatccgtcc acaagaaatc tctactcaag ttctccctcg ggatctacat
    23461 gctgaatatt tctcagcgat ggcgcttatt gctacaagta ttgaacgttt tgcaacagag
    23521 atacgcggat tgcaaaaatc cgaaacacgt gaagtagaag aattttttgc gaaaggtcaa
    23581 aaaggttctt ctgctatgcc gcacaaaagg aatccgatcg gttcagaaaa tatggctggt
    23641 ctagccagag tggtgcgcgg acacatggtc actgcctatg aaaatgtcac attatggcat
    23701 gaaagagata tttctcattc ttcagcagaa cgaatcatct tgcctgatac cacgatttta
    23761 ttgaattaca tgttaaatcg ttttggcaat atcgttaaaa atctaacggt ctttcctgaa
    23821 aacatgaagc ggaatatgtc tgcaacattt ggtttgattt atagccagcg cgttatgctg
    23881 aaattgatcg ataagggaat gacaagagaa gaagcttatg atcttgttca gccaaaaact
    23941 gcgtacgctt gggacaacca agtgcagttc cgcccgctgt tagaagaaga tccaaccatc
    24001 acatcggtat tatctaaaga agaaatagac gatgcctttg attatcatca tcatttgaaa
    24061 aacgttgacg tgatattcaa acgagtaggt ttggaataaa gaagagtgag ttgcaaaatt
    24121 caattaaaat agaatagcaa aaataaccaa aaaagaagca gatccgttag cggttctgct
    24181 tcttttagtt tattatattt tcttatcaat aataggaacc tgattttaaa agcggctacc
    24241 tctttaccgt gcctctttac atacaaaaaa tagaggaatt aagcgtaaat ggcttggcta
    24301 ttataactaa aatgctggtt atggagatgt tgcagcaagt tttcgagaaa agcgtattcc
    24361 atctcttgtc cattgaataa gatctggata cgataatgat ttttgaaacg cttctgttca
    24421 tgaatctgac tgattgaaag ttcgcctttg tgttctgtct gataaagaaa agtcctttta
    24481 cggcattcag cacagcgctt ggttgctcgg cggatttttc ggaatgtata aaaactagta
    24541 tctttattgc ataaataaac gtctttgatc gacagcaaat cattatatag acaggaacgt
    24601 tcttcagctg ttaattctga taaaaaaagc tgaccttgtt ttattgcatc ttccatcggc
    24661 aagtgcctcc tcgaacccat tatccaaatt tttagttaat tctaatatac catcattaag
    24721 tataaatgta aacgcttgtt gatggtataa gattttcgtt tctgataaaa cacgaacatt
    24781 aaaagaatct aatgccttaa atgtacatat ttttgttgat ttataaaaag atgattgcta
    24841 aaatttaaag tgaaaaaacg aacaaaaaac gatgtatttg cttttaatgt aaataaaaag
    24901 gagagacgta catggagaag aaagaacttt tgtatgaagg aaaagccaaa aaaatatatc
    24961 ttacttctga tgagaacttg atttggatag aatatttaga tcaagcaact gcgttgaatg
    25021 gcgcgaggaa agaccagata agtggaaaag gagaattgaa taatcaaatc acaagtgcaa
    25081 ttttccgtta tctgactcaa agaggaataa aaaatcattt tattgaacaa ttgtctaaaa
    25141 ccgaacaatt agcaaaaaaa gtacaaatta tccctctgga agtggttatc cgaaatattg
    25201 cagcaggaag tttctcaaaa cgtctaggtg ttcccgaagg taaagaattc atcaaaccaa
    25261 tcattgaatt ctactataag gatgaccagt tggatgatcc atttatcaat gaagagcata
    25321 tctatttctt acaacttgca aaaccggaag aaatcagtga gctgaaagcc caagcgttgg
    25381 ctattaatga aattttgacc gaattatttg ctaaggtcgg tttggtttta gtagatttca
    25441 agctagaatt tggccggtcg aatggacaaa ttttgcttgc ggatgaagta acaccggata
    25501 cttgccgatt atgggatgaa cgtacgaatg aacatatgga caaagacgtt taccgcagag
    25561 atctaggtga tttgatccca gtctatcaag aagtatataa ccgtctatcg gaactaactg
    25621 aagaaaagga gcgagtatga tgtatttcgt aaaagtatat gtaacctata aagattcagt
    25681 gttagatcct caaggggagg cagtaaaaaa agcagtccat cgtatgggat atgaaacaat
    25741 cgaagatatc cgaataggaa aatactttga gatacaactt gctgatgcag aagaaccaat
    25801 cgatacagtg attgaaacga tttgtgacaa gctgcttgca aatgtaaata tggaaacata
    25861 ccgatatgaa atcaaaaaaa tggaggaagt ataacatgaa atttgcggtc atcgtttttc
    25921 caggttcaaa ttgtgacgca gatatgttat gggcagttcg cgagatcatg ggcgctgatg
    25981 ccgaatttgt acggcatgat gagtcctctt tagaaggatt tgatggggtt ttactgccgg
    26041 gaggattctc ctatggtgac tatttgagat gcggtgcgat tgctcggttt tctacaatca
    26101 tggaagaagt gattcgtttt gctgaagaag ggaaaccagt attcggtaca tgcaatggat
    26161 tccagatatt gacagaagca ggacttttgc caggtgtact gagacgaaat gagtctttgc
    26221 attttatttg caaaactgta ccattgaaag tcaaccccaa gacggccttt acttcagagt
    26281 atgaagccga cgaaataatc tatcttccag ttgctcatgg ggaaggaaat tatttctgtg
    26341 acgaagaaac acttcaagaa ctaaaaaaaa ataatcagat tgcctttact tataatgaca
    26401 acatcaacgg cagcgtggaa aacattgcag ggattacgaa caaagcaggg aatgtgttag
    26461 gaatgatgcc tcatccagaa cgtgcagtag aagacttgct aggatcagaa gatgggaaac
    26521 ggttctttgc ttccatttta aaaaacttcg gaaaggtgac cgtatagatg aaaacattga
    26581 aagaaccgac agctgaagag atcaagaaaa aaagaatcta tgccgaatgg ggattgactg
    26641 atgaagaata tcgaatgatc gaagaagata tcttaggaag aatgcctaat tatacggaaa
    26701 ctggactgtt ttctgttatg tggagcgaac attgttccta taaaaattct aaaccggttc
    26761 tacgcaaatt tcctactagt ggacctcatg tgttgcaagg ccctggagaa ggcgcaggca
    26821 tcgtcgacat cggtgatgga ctagcagttg tcttcaaagc agaaagtcac aatcatcctt
    26881 cagccgtgga gccttatgaa ggagctgcga caggagttgg cggaatcatc agagatattt
    26941 tcagcatggg ggctcgtcca attgcgttgc tagattcatt gaggttcggc gaattagata
    27001 atccaagaac gaaatattta ctagaagaag tagttgcagg tatcggcgga tacggcaatt
    27061 gtatcggcat accgactgta ggcggcgaaa ttgcatttga tccttgttat gaaggaaacc
    27121 cattagtcaa tgcaatgtgc gtaggtttga ttgaacacaa acatatccag aaagggcaag
    27181 ccgctggcgt cggtaattcg atcatgtatg taggtgcaaa aacaggtcgc gacggtatcc
    27241 acggagcaac ttttgcctca gaagaattta atcaagaaga agaacagcaa cgttctgctg
    27301 tacaagtagg cgatcctttt atggaaaagc tgctgcttga agcgtgtttg gaattaatct
    27361 tgcatcattc ggattctcta ataggtatac aggatatggg agcagcagga cttgtttcat
    27421 ctagtgcgga aatggcttca aaagctggct caggacttat attgacatta gatgaagtcc
    27481 cacaaagaga aactgggatg acaccttatg aaatgatgtt atcggaatca caagaacgga
    27541 tgttgatttg tgttaaatca ggagaggaag aaaaaatcca agaactattt cagaagtatg
    27601 atctagatgc agtcactatc ggtaaagtaa cagacgacgg gcaatatcgg ctatatcatc
    27661 aaggagaaga agtagcaaat ctgccagtcg atgcattagc tgaagatgca ccagtttatc
    27721 ataaagaaat gaaagaacca gctcgaatcg tagaatttca gcagttagct ccttatcagc
    27781 cagttatcga agaacctgga caagtattgc ttgatctgct gcagcaaccg acgattgctt
    27841 caaaacgttc aatctacgaa acttatgatt cacaagtaca aaccaataca gttgtccgtc
    27901 caggaagcga tgctgctgtg atgagggtta gagggacgaa taaagcgtta gctatgacca
    27961 cagactgcaa cgcgcgctat ctgtatttgg atccaaaaat cggaggacaa atagcagtgg
    28021 cagaagctgc tagaaatatt attgctagcg gtggtaagcc cttggcgatc accgactgcc
    28081 tgaactatgg ttctcctgat aaaccagaag ttttttggga attatcgact tcagctgacg
    28141 gaattgctgc tgcgtgtgaa acgttaggaa ctcctgttat ttcaggaaat gtctctttat
    28201 ataatgaaac ggatggtcaa gcaatctatc caacaccaat gattggtatg gtcggattga
    28261 ttgaggatca taaacatatc actactcagg aattcaaaaa atcaggggat ctaatctata
    28321 tattaggtaa gacgtttgca gattttgatg gcagtgaatt acaaaagatg cagcttggaa
    28381 gaatcgaagg tgtgatccga aactttgatc tatccattga aaagagaaac caagagttag
    28441 tattgacagc catccaaaac ggattgattg aaagtgctca tgactgttca gaaggtggct
    28501 tggcaattgc actggcagaa tcagctttca aacatcagct agggatatct gtacagtttg
    28561 aattgtcctc tgctcaatta tttgcagaaa ctcaatcaag atttgtgttg acagtcgcac
    28621 cggaaaataa gacacgattc gaagaaatga tgggagatgc tgctgtttta gcaggtaagg
    28681 tgacggatga agcgataatc gaaatttcag caactgacgg tcagatcaaa atagaaactg
    28741 cagtagcaag aaagtgctgg gaggatgcaa tcgtatgtct tatgaagtga agagtttaaa
    28801 tgaagaatgc gggatcttcg gtgtttgggg tcatccagac gccgctagtg tggcatactt
    28861 cgggctccat agtcttcagc atcgaggaca agaaggtgca ggtatcgtgt ccaatgatcg
    28921 aggaaagctt aaggggcatc gagatttggg gctgctttct gaggtattca aagatcaaag
    28981 aaaattgcag cagttaacag gagaagctgc aatcggacat gtacgttatg caacagcagg
    29041 caatggaagt gtggacaata ttcagccttt tctattcaaa tttcatgatc agtcgatcgg
    29101 cttagcgcat aatggtaact tgatcaatgc tcgttccctt agaaaagagt tagaggaaaa
    29161 tggggcgatc ttccattcta actccgatac ggagatattg atgcatttga tccgacgaag
    29221 tgaagaaaca gattttttaa ataaattaaa aagtgctttg aatgaagtca aaggtggctt
    29281 tgcctattta ttaatgacag aaacggcaat gattgccgct ttggacccaa atggttttcg
    29341 tccattagca atcggacaaa tgaaaaacgg agcatacgtg attgcctcgg aaacttgtgc
    29401 attagaagtt attggggcac gttttataag agatgtccag cctggtgaga tagtgatcat
    29461 caatgacgaa ggaatccaga ttgattgctt tacgaaggac acgcagcttt ctatttgttc
    29521 gatggaatat atctattttg ccagaccaga ttcaaacatc gcagggatca atgtgcatac
    29581 tgcacggaaa aatatgggac ggaatttagc gaaagaatcg ccagtaaaag cagatatggt
    29641 cgttggcgta cctaattctt ctttgtcagc cgcaagcggt tatgcggaag ctagtggaat
    29701 cccttatgaa atcggattag taaaaaatca atacattgcc cgaacattta tccagccaac
    29761 gcctgagcta agagagcaag gtgttcgaat gaaattatct gctgtacgag gagtagtgga
    29821 aggtaaaaag gtgattatgg tcgatgattc aattgttcgg ggtacgacaa gcagacggat
    29881 cgtccatctt ttgaaagaag caggggcaaa agaagtccat gtgaggattg cttctcctcc
    29941 tttgaaatat ccatgttttt acgggattga tattcaaacg cgtaaagaac tcattgcggc
    30001 aaatcacacg attgatgaaa tcaaagaatg tatcggtgca gattctttag gttttttaag
    30061 tgaagagggt ctgatcgaat cgatcggtct ggaaaaagat gctccgtatt caggattgtg
    30121 tatggcttat ttcaacggag attatccgac gccgttatat gactatgaag aaaaatatca
    30181 ggaatcacta aaagaaaaag tatcgttctt ttaaaagcag accaagaagg gagtatatga
    30241 attcaatggc aaatgcatac gcaaaagctg gcgtagatgt cgaagctgga tatgaagtag
    30301 tagaacggat caagaagcat gtcaaaaaaa cggaacgttt aggtatgatg ggcgctatag
    30361 gaggattcgg cggttgtttt gatttatcgg tctttcaatt aaaagagcca gtccttgtct
    30421 cggggaccga tggcgtgggg acgaaattag cagtggcaat cgaagcagac aaacacgata
    30481 caatcgggat cgactgcgta gcgatgtgtg tcaatgatat cttagcacaa ggcgcagagc
    30541 cgttatattt cttagactac ttagcaacgg ggaaaaatga tccgttacgt ttggaaaatg
    30601 tagtaaaagg tgtagcagat ggatgtgttc aaggaaatat ggcgctgatt ggcggagaaa
    30661 cagctgaaat gcctggtatg tactcaggta atgattatga tctagccgga tttgctgtag
    30721 ggatagctga aaaatctcaa ttattgacag gagaaacgat caacccagga gatgtgttgc
    30781 ttggaatagc atctagcggg attcattcaa atgggtactc tcttgttcgc aaaattttct
    30841 ttgagcagca gcagttttct ttatcatcaa aactgccaga attgaaaaaa acacttggcg
    30901 aagaattgct tgagccaacg aaaatctatg tttcttcctt gcttccgctg atcaaagaaa
    30961 agaaaataaa aggagctgca catatcactg gtggagggtt tgtagaaaat atcccgcgaa
    31021 tgttaccaga atctatggct gcacaaatca aacttggctc atggccgatt cttccaatat
    31081 tcggctgttt acaaaaatat ggtgaaattc ctgagatgga gatgtatgaa atcttcaata
    31141 tgggcatcgg tatggtcctg gcagtcgaag aatcgaaagt aaaagaaata gtacacgaaa
    31201 ttgagcaagc tggcgaaaaa gtttatgaaa tcgggaaagt gatcgccaaa gaaaaacatc
    31261 gtgtggtgtt tataggagaa gaacgatgag aatcgctgtt tttgcttctg gaaacggaag
    31321 caactttcaa gcgctagccg attatctatc gaaaaaagga ttggaatctt cgattgactg
    31381 gttgttttgt gatcagcctg aagcatatgt tttgaaacgt gcaactgcgt tgagtgtacc
    31441 ggcagattgt ttttcaccta aagaatttga ttcaaaaaaa gagtatgaag aagcaatttt
    31501 gcataaattg aaagaaaaaa agatcgattt gatcgtatta gctggatata tgcgaatcat
    31561 tggaccggta ttgctgaaaa attatgataa aagaatcatc aatatccatc cgtctctttt
    31621 acctgccttc cctggtttgc atggcatacg tgatgccttt gaagctggag taaaagaaac
    31681 gggtgtcaca attcattata tcgaccaagg tgtagatact ggaccaatca tccggcagga
    31741 aaaagtgaga attgaacagg aggacacgtt cgattcttta gaagaaaaaa tccatagagt
    31801 agagcatcga atctatccag aagtgattag tgaaattatt gaaaatggag gctatctgaa
    31861 atgacaagag cattgatcag tgtatcagat aaaactggga ttgtagcatt cgcccaaggt
    31921 ttgattgaac aaggagtaga aatcatttca acaggaggaa caaagcatgc gttggataaa
    31981 gctgggatca aaacgctttc gatcgaagaa gtgacagggt ttcctgaaat gatggatggc
    32041 cgtgtcaaaa cacttcatcc taaaatccat ggtggtcttt taggaagacg cgatctccct
    32101 agccatatgg aggcaatgga aacggaaaat atccaaccaa tcgactttgt ttgtgtcaat
    32161 ttatatcctt tcaaagaaac gattatgaaa ccagatgtcg aaatagcaga agctattgaa
    32221 aacattgata tcggtggtcc tagtatgctc cgtagtgcag caaaaaacca tgaatttgtc
    32281 acctctgttg ttgatccatc tgactatgaa gaagtacttt cagaattacg tcagtatggg
    32341 catacttcac tttctttcag acaaaaacta gcggcaaaag tctttcgtca tactgcggca
    32401 tatgatgcac tgatcgctca atacttaacg gattgggtgg aagaaaaaga accggaaaaa
    32461 ttgacactta cgtatgaacg aaaacaaacg cttcgttacg gtgaaaatag ccatcaagcc
    32521 gctgcctttt atcagtccgc tcttctggaa aaatattcga tcgcagcagc aaaacaactc
    32581 catgggaaag aactttctta taataatatc cgagatgcag atgctgcgct acggatcatg
    32641 aaggaatatg acgagccaac ggtagtcgcc ttgaaacaca tgaatccttg tggtattggg
    32701 acagcatcta cgattttaga agcgtgggaa gcagcgtatg aagcagatcc tgtttcaatc
    32761 tttggtggga tcatcgtttt gaatcgagaa gtagatctgc caacagcaga acagatgcat
    32821 aagctgtttc tagaaatcat cattgcccct tcttatacac aagaagcttt ggaattactg
    32881 caaaaaaaga aaaacatccg attactgaca gtagactttg cgcaggaaga aaatcaaaat
    32941 cgagaagaaa cggtctcagt cttaggtgga cttcttgtac aagatcaaga tctggcaata
    33001 gaaaatgaag aagagtggaa agtcgtgaca aaacgtaagc cgactccagc agaagcaaaa
    33061 gcattagcgt ttgcttggag agctgtcaaa catgtgaaat ctaatgcgat cgtactggca
    33121 aacgaacgtc agacagtagg aatcggagcc gggcagatga atcgagtcgg ttcagtaaaa
    33181 attgcgattg aacaagcgca agagaaaatg gaaggtgcag ttttagctag cgatgcttat
    33241 tttccaatgg atgattcggt tgaatatgca gccaaacatg gtataaaagc aatcattcag
    33301 ccaggaggaa gcatcaaaga ccaagattct attgatatgg cgaataaata cgatctagca
    33361 atggtcttta ctgatgttcg gcattttaga cattagagag gagtcggata aatgaacatt
    33421 ttggtgatcg gtgcaggtgg tcgtgaacat gcaataagta aaaaactttt agccagtccg
    33481 aaggtggata cggtgtattg tgcgccagga aatccgggga tgacaaaaga tggtatcaat
    33541 tgtgtcgcta tcagtgagcg agatcattca gcgttaatcc attttgttaa gaaagaggct
    33601 attgattgga gtatcgttgg tcctgaagtt cctttgttaa atgggatcgt cgatgatttt
    33661 atggcggaag gattgaaaat cttcggtccg acaaaagcag cagctctgat cgaaggttcg
    33721 aaaagttttg ctaaacaatt aatggatact tatcaaatcc cgacagctaa atccagaagt
    33781 tttgaatgtt acgaaaaagc agcagcgtat atcaaagaaa atggtgcgcc aatcgtagtg
    33841 aaagcggacg gcttagcagc tgggaaaggt gtagtcgtgg ctaccacagt gaaggaagcg
    33901 ctggatgcgg cagaacaaat gttgaaccag catcgatttg gggatagtag caaaaaaatc
    33961 gtcgtagaag agtttttggc aggagaagaa ttctcccttt tggcgtttgt ccgtaatacc
    34021 aaggtgtatc cgatggtcat ttcgcaagat cataagcggg catttgataa cgataaaggg
    34081 ccaaatacag gcggaatggg tgcttattca ccagtaccgc aaatttcaaa aaagatgatt
    34141 gaaaaagctg tggaagaagt tttaaaacca gcggctttgg gaatgaaaga actaggacgc
    34201 tctttcacgg gtgttttata tgcgggtttg attgctacga aggaaggacc aaaagtgatc
    34261 gaattcaatg cgcgtttcgg tgatcctgaa acgcaagtcg ttctttctcg gttgaagagt
    34321 gactttgcgc aagtaatcga tgatttatta gagaatcgta cagtagagtt aaagtggcag
    34381 caagaaggat acaatgtagg cgttgtggtt gcagctgccg gttaccccga agaatatgag
    34441 acaggaatgg ttctaccaga ttttctggaa gaagagcttt ctgtttacta tgctggagtt
    34501 tcctcaaata acggcaaact agttggagca ggaggacgtc tttatttagt agaagcatac
    34561 ggtgagacga tcaaagaagc acagaaaaaa atttacaccg cgttaatgga taaaacgaca
    34621 gatggaacat tttatcgtac agatattgga gaaaaagctg ccaatagcga actgatcgtg
    34681 aagtaaatca gctgcttcca tttataaaaa atacaacaaa cataatcgga agcgccgatt
    34741 ttagtgaagc cttttcataa agagtgtggt acacttttgt tgtgtatata aataaggagg
    34801 agttcagatg aaaaaaattc aattaggtac ttctgatcag aaagcagctg cagtgatctt
    34861 aggctgtatg cgtatcaaca atgcggaaga tccagtaaaa gtgattgaga cagcttatga
    34921 aaacggcatt gatttttttg accatgcaga tatttatgga aacggtgaat gtgaagaaat
    34981 ctttgcagat gctctagcaa aaacatcaat caaacgagaa gatcttttta ttcaaacaaa
    35041 atgtggaatc gttccaggtg tgatgtttga tttttctaaa aaacatatca ttgaatctgt
    35101 agaaaaaagt ttgacccgtc tgaaaatgga ttatgtggat gctttacttc ttcatcgtcc
    35161 agatacattg gtagaaccag aggaagtagc tgaagcgttt gatgaattgg aacggtcagg
    35221 aaaagttcgt tattttggcg tcagcaatca aaaaccaatg caaatcgaat tgctgaaaaa
    35281 atatgttcgt caaccattag tagctaatca gctgcaattt ggcttgaaac atactggaat
    35341 gatcgatcaa gggattcatg tcaatatgac agatgaagca agtatcgatc atgacggtag
    35401 tgtacttgat tattcccgat tgcatgatat gacgatccaa gcatggtcac cataccagta
    35461 tggatttttt gaaggtgtat ttatcgggaa cgaaaaattc cctgaattaa accaaacatt
    35521 agaagaacta gcagctaaat atcaaacgac accaactggc ttggcttctg cttggatttt
    35581 acgccatcca gccaatatgc aagtgatcgc cggtacgatg aacgtagggc gaatcaagga
    35641 aatcgctcaa gcttcagaaa ttaatatgtc tcgggaagat tggtacgaat tgtaccgtgc
    35701 tgctggaaac attttacctt aaagattgtc ataagcaggt caaaagggct ataaattaaa
    35761 aagacagaat aacttgaaag ctgttttcag cttcg
//
LOCUS       DACEDM010000026        35709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEDM010000026 DACEDM010000000
VERSION     DACEDM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.026"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..1282)
                     /locus_tag="IWO50_001141"
     CDS             complement(5..1282)
                     /locus_tag="IWO50_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358275.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="IWO50_001142"
     CDS             complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="IWO50_001142"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0358276.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2204..3148)
                     /locus_tag="IWO50_001143"
     CDS             complement(2204..3148)
                     /locus_tag="IWO50_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0358277.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3150..3836)
                     /locus_tag="IWO50_001144"
     CDS             complement(3150..3836)
                     /locus_tag="IWO50_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0358278.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4310..4630
                     /locus_tag="IWO50_001145"
     CDS             4310..4630
                     /locus_tag="IWO50_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0358279.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4784..5689
                     /locus_tag="IWO50_001146"
     CDS             4784..5689
                     /locus_tag="IWO50_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0358280.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            5686..6459
                     /locus_tag="IWO50_001147"
     CDS             5686..6459
                     /locus_tag="IWO50_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0358281.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(6640..8064)
                     /locus_tag="IWO50_001148"
     CDS             complement(6640..8064)
                     /locus_tag="IWO50_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0358282.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            8275..8721
                     /gene="nrdI"
                     /locus_tag="IWO50_001149"
     CDS             8275..8721
                     /gene="nrdI"
                     /locus_tag="IWO50_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0358283.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(8786..10768)
                     /locus_tag="IWO50_001150"
     CDS             complement(8786..10768)
                     /locus_tag="IWO50_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0358284.1"
                     /translation="MAKNELTLEELAKHIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="IWO50_001151"
     CDS             complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="IWO50_001151"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0358285.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(11681..12742)
                     /locus_tag="IWO50_001152"
     CDS             complement(11681..12742)
                     /locus_tag="IWO50_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0358286.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(12953..13480)
                     /locus_tag="IWO50_001153"
     CDS             complement(12953..13480)
                     /locus_tag="IWO50_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0358287.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(13547..15049)
                     /locus_tag="IWO50_001154"
     CDS             complement(13547..15049)
                     /locus_tag="IWO50_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0358288.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(15307..16428)
                     /locus_tag="IWO50_001155"
     CDS             complement(15307..16428)
                     /locus_tag="IWO50_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0358289.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            16554..17495
                     /locus_tag="IWO50_001156"
     CDS             16554..17495
                     /locus_tag="IWO50_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0358290.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(17645..18541)
                     /locus_tag="IWO50_001157"
     CDS             complement(17645..18541)
                     /locus_tag="IWO50_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0358291.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            18810..20150
                     /locus_tag="IWO50_001158"
     CDS             18810..20150
                     /locus_tag="IWO50_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0358292.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20368..20811
                     /locus_tag="IWO50_001159"
     CDS             20368..20811
                     /locus_tag="IWO50_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0358293.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="IWO50_001160"
     CDS             complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="IWO50_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0358294.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(23136..24359)
                     /locus_tag="IWO50_001161"
     CDS             complement(23136..24359)
                     /locus_tag="IWO50_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0358295.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(24390..25229)
                     /locus_tag="IWO50_001162"
     CDS             complement(24390..25229)
                     /locus_tag="IWO50_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0358296.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(25246..25926)
                     /locus_tag="IWO50_001163"
     CDS             complement(25246..25926)
                     /locus_tag="IWO50_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358297.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(25928..27001)
                     /locus_tag="IWO50_001164"
     CDS             complement(25928..27001)
                     /locus_tag="IWO50_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0358298.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMKLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(27648..28318)
                     /locus_tag="IWO50_001165"
                     /pseudo
     CDS             complement(27648..28318)
                     /locus_tag="IWO50_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            complement(28405..31083)
                     /gene="mgtA"
                     /locus_tag="IWO50_001166"
     CDS             complement(28405..31083)
                     /gene="mgtA"
                     /locus_tag="IWO50_001166"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0358299.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            31742..32179
                     /gene="ndk"
                     /locus_tag="IWO50_001167"
     CDS             31742..32179
                     /gene="ndk"
                     /locus_tag="IWO50_001167"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0358300.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(32201..33067)
                     /locus_tag="IWO50_001168"
     CDS             complement(32201..33067)
                     /locus_tag="IWO50_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0358301.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(33227..35320)
                     /locus_tag="IWO50_001169"
     CDS             complement(33227..35320)
                     /locus_tag="IWO50_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0358302.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        10340 a         7497 c         6297 g        11575 t
ORIGIN      
        1 ttttttatag taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt
       61 ctaacttctg ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc
      121 ctttccattt gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt
      181 gccatttttc ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt
      241 ctttacgcaa tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc
      301 cttctgtaaa accatagcga caatattgct tccaaataaa tagatagatt tttggttgga
      361 tgaatcgttg gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac
      421 gttttgtctt tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca
      481 cttcttcata tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga
      541 attcttctac atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct
      601 gtcgcttttt tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg
      661 cttgataatc cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag
      721 ctaattttat atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac
      781 agtatttttg ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt
      841 cgtctaaggc cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag
      901 agacatgcag gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg
      961 gcgatttaac gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct
     1021 ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg
     1081 cagctaaata atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga
     1141 ctagttcccg ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt
     1201 attgtttaga aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa
     1261 agacggattg ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta
     1321 ctaacataac gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat
     1381 ttccggaaat atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag
     1441 caatgtcttc taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg
     1501 agaatggcac gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa
     1561 gcaaacgata catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg
     1621 gtagaccatg atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag
     1681 cggctggacc gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga
     1741 ttcttgcttc tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg
     1801 cttgtccgac acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt
     1861 gactgctttt ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt
     1921 catctggcat tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat
     1981 caaaacctgt tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg
     2041 ttatctttta tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat
     2101 aacataacag atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta
     2161 gaagaggttg cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca
     2221 tataagcaat ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc
     2281 cgtatttgtc tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg
     2341 gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat
     2401 tcgctccatg actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg
     2461 caaatgcatt ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc
     2521 cgatgttcac atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa
     2581 ctccagcgct tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg
     2641 gccctactgt gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc
     2701 gagaaaagaa catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag
     2761 cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa
     2821 taaaaatttt cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc
     2881 ggctttctcc tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc
     2941 caccgttcac gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc
     3001 agaaaaaggc tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag
     3061 tgtaaatcaa tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata
     3121 aaatatcagg atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc
     3181 gctaatttct tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc
     3241 actggaactt ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt
     3301 gttgattgga ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag
     3361 cagacatttt ccatagctga agccatacct tttagttctt cttcatgctt aggatggact
     3421 tctaaccctt ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat
     3481 ccagcaactc caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa
     3541 atatctacaa tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat
     3601 tctttcagac catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc
     3661 atcccgcgat tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga
     3721 ccgccagtca aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta
     3781 aatgcaaacc cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat
     3841 tttcctccat aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag
     3901 ttgttttctc acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa
     3961 aattatctta cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga
     4021 aataaggtct agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa
     4081 attgcaaagc attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt
     4141 tactaaaaag tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata
     4201 tacaatctgc caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat
     4261 gattttcctc tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat
     4321 ggaattaatc atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga
     4381 tggtttttct aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta
     4441 ctttttgtca aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg
     4501 gattggcgca gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct
     4561 ttccacttgg ttctttgtgg ggatgctact catcagtatc attggcatca agattactag
     4621 cggccactag acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat
     4681 tcaatttttt tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat
     4741 caggtgtatg cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt
     4801 ccaagaatta tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt
     4861 atcccctggc aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat
     4921 gaaagctatt ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga
     4981 gatttctccc tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg
     5041 tatctatcca ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt
     5101 atctgctatc gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc
     5161 gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca
     5221 tccgtctatc gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga
     5281 tgtgcgaaaa atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca
     5341 cttactcagc gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat
     5401 catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc
     5461 tacaacaaat gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga
     5521 tcctgataaa atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct
     5581 attgtctgca caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc
     5641 ttctctattg gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta
     5701 aaacaagaat tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta
     5761 gttgttttgc tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca
     5821 gttgaaatga ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca
     5881 gctgcaagca cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat
     5941 cgaaagtatt ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct
     6001 gtttatttat accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct
     6061 tcgatttcct tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact
     6121 tatactttgg gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct
     6181 tgctttatca atagttccgg cgcatccatc tcagcaggaa tcgtctttta ttttgcttcg
     6241 tcaatcatat ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat
     6301 ccgatcagta tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg
     6361 actcatcttt ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta
     6421 gcattaggat atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct
     6481 gtgaaagaga cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga
     6541 aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac
     6601 gacaggctca caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt
     6661 ggcaaataaa accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc
     6721 taagtggata tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat
     6781 gcctggcaag aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc
     6841 ccatacttga aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg
     6901 ataagaaagt ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt
     6961 accaagtgtg taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac
     7021 attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt
     7081 tccccatgct ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa
     7141 gatcgtccat gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac
     7201 tgctccccat ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat
     7261 cgtttgcagc tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat
     7321 aataatcaag ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc
     7381 tcgtgaacct acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat
     7441 gcaactcaat acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc
     7501 gctagaaaag ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa
     7561 taaaaataat ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc
     7621 gactccccga ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga
     7681 tgccagattt tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa
     7741 tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa
     7801 accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg
     7861 acgtttttta ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag
     7921 tacccaactg aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa
     7981 tacgccacct ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc
     8041 ttttttctgt tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa
     8101 aagtataaca aacctttttt atttttactt aattaataaa aactttaata gaaatataat
     8161 tctaccggct tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca
     8221 ccaaaaaact taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac
     8281 attttatata tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat
     8341 gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat
     8401 gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta
     8461 gacggcggaa atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa
     8521 tatctggaac atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa
     8581 aacttcaata atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct
     8641 ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt
     8701 ttagcagaaa acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc
     8761 tttcgtttct attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt
     8821 ttgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga
     8881 cgatcatgga tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa
     8941 gcgtatcagg tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta
     9001 agtcaactgt gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc
     9061 caatagcgtg tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat
     9121 gtccgttttc tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat
     9181 cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac
     9241 tttcagctga agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag
     9301 tcgctaattc ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga
     9361 agccaccagc ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga
     9421 tcaatgggat caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca
     9481 ttccgatcac tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta
     9541 gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag
     9601 ctcctttgat catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg
     9661 ctgcacccac ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag
     9721 tttcatcaat catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca
     9781 tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc
     9841 agttgatccc accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa
     9901 tttcaatcag accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat
     9961 caggtactac tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga
    10021 cacctaggat cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg
    10081 gtgcttccgg attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg
    10141 gtgttgcacc aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca
    10201 tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg
    10261 ttccggctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga
    10321 caaatgcagg aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt
    10381 tagatggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac
    10441 ggtcttttcc agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc
    10501 ctaatttcac gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc
    10561 cgatgatgac ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta
    10621 attcagagac atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt
    10681 ggacaatgct ttctacattg cccatgccgc ctgccccttt ataaatgtgt tttgccagtt
    10741 cttccaaagt cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca
    10801 aatccgtttg cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc
    10861 atgacgatag ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg
    10921 taatcagctt ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt
    10981 gttggtttga catcgaccat caagttattg aatactttac ctataccaat catactgata
    11041 gtagaaatca tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct
    11101 gtcaaaaatt ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg
    11161 gcttgtttat tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat
    11221 ccaccgatca catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt
    11281 gtcaaattca gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg
    11341 acagctacag tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct
    11401 acaccgaatg tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg
    11461 gctcccatat aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact
    11521 ggttcgatac ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc
    11581 attagttgaa cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg
    11641 cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta
    11701 taaattgata gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa
    11761 ctttagctac tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct
    11821 cacccatata gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag
    11881 ctgtatttct tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga
    11941 catctcttgc atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt
    12001 catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct
    12061 cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta
    12121 atagatctag agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc
    12181 cttgatatag tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac
    12241 cttgaatttt gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg
    12301 gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca
    12361 attcatcgat atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg
    12421 accagtcggc aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa
    12481 ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg
    12541 aaatatcgac catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc
    12601 gcttttgata tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa
    12661 agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca
    12721 taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg
    12781 actagagtat atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc
    12841 atttctaaag gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc
    12901 taagcaaaag cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat
    12961 aagaaaccaa ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat
    13021 gaaaataagc attgatcact cttatcaagg cattatggca aactaacaga tagacatttt
    13081 cttcgtcttc gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg
    13141 actccccatt tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt
    13201 tgcttggcag tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca
    13261 gcggcaagct gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg
    13321 aatggatgat tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg
    13381 cctgcatctg tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg
    13441 cgttccaagt tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg
    13501 caaagttccc cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa
    13561 gagaaataat ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca
    13621 tggcaggttt cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat
    13681 aattcaatgg ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt
    13741 ccactttttt ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt
    13801 ccatcccaag ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg
    13861 caaattccgc caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca
    13921 gatgttttgg ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat
    13981 ggataatgga tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct
    14041 cgattgtaaa atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta
    14101 cgcttaaaag tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt
    14161 cgatatcttt taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg
    14221 ctttggatcg tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc
    14281 tagattcagt atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag
    14341 accattcggc tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt
    14401 cattttgagc catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat
    14461 tcccttgata aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat
    14521 cgataaatcc ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag
    14581 ccgctacttg ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga
    14641 aatgagtgtc atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata
    14701 gtgtgctgaa ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt
    14761 cgatttccca tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt
    14821 tatcttttgt ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat
    14881 aaggtagttt gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga
    14941 taagtcctag tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc
    15001 cttggctgtc gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc
    15061 tcgtaaaaaa agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata
    15121 cacacaaaaa gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct
    15181 tccttgtctc cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa
    15241 tgcacggatc cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt
    15301 tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt
    15361 tttttctaat gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat
    15421 acttaagaca attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca
    15481 aatagcgatt attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt
    15541 tccgtggatc gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc
    15601 gatctttttt tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc
    15661 tcccaacaaa aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc
    15721 taaatccacc tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa
    15781 gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg
    15841 ttgacctgta tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag
    15901 aacgtttctt cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta
    15961 cccttctaaa gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct
    16021 tcctcctaaa gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc
    16081 tcctaaggca taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc
    16141 taaccccaat gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa
    16201 taaatagctg atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt
    16261 tcccctgagc gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac
    16321 attgattttt cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa
    16381 tgaagaagca cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca
    16441 ctttcttctt aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct
    16501 tccatttttc tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa
    16561 tcgcaatagc tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg
    16621 gaaatgaggt cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg
    16681 ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag
    16741 aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc
    16801 aattagatga tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt
    16861 gtttactaaa tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata
    16921 tcttatttgg gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt
    16981 taggtgatgg agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa
    17041 aagttgtaga agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt
    17101 attccatttg gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg
    17161 attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg
    17221 tatctgaatt tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt
    17281 accaacatat tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt
    17341 accaagacct gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt
    17401 ggcgaaaagg acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag
    17461 tccatgcaaa agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg
    17521 aatcagcagc tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc
    17581 tcatgatgcc ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg
    17641 ttttttattt gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat
    17701 cgttagtgat aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata
    17761 ggattggctt aaatggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa
    17821 gcaaaaaaat attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca
    17881 ctagtttctt ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct
    17941 cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag
    18001 taagatatcc aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat
    18061 ctctttttat aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg
    18121 caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa
    18181 atgaactatt atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct
    18241 catcgattgc cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt
    18301 gattccaaaa aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct
    18361 ccatatcaaa tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag
    18421 ctgatttaat gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga
    18481 tggaaggcaa aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca
    18541 tcttttccgc ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa
    18601 agccagtaaa acgtttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg
    18661 aaataaatca ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct
    18721 gaaaatttgt tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg
    18781 attgtgaatt taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta
    18841 gctccagaca tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag
    18901 gaagtggcga agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg
    18961 ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca
    19021 cgcatcacgg attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta
    19081 ctgattgggt ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg
    19141 cttccttttt gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg
    19201 ctgttttaac tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt
    19261 ggctggcttt tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg
    19321 tcggtcagca aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg
    19381 gtgggtttac tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt
    19441 cttacggcgg ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg
    19501 cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt
    19561 tgtttttgct tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt
    19621 ttgtgatggt atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga
    19681 ttgtccttgc tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt
    19741 tcttccgttt gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc
    19801 aagtaccgca acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt
    19861 cttattttgt cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat
    19921 tattgatctg gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat
    19981 tatgggataa aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta
    20041 tcctgttcac gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt
    20101 atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga
    20161 aaattttatt ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa
    20221 gtcttgttgc aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt
    20281 caaaaaccct ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa
    20341 ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc
    20401 ggtgtttcaa cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat
    20461 atcttcttca gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc
    20521 atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta
    20581 gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc
    20641 aacgacacag cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt
    20701 aaacgagctg atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca
    20761 caagaagaag agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc
    20821 gtccaaataa aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct
    20881 acttctggaa cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga
    20941 acagccaaac aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg
    21001 caagcttttg aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta
    21061 gagagacaaa tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg
    21121 tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt
    21181 aagaaaaatt ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga
    21241 aactggcttt tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat
    21301 ccctttttat gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc
    21361 acaaattttt ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa
    21421 agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct
    21481 acgaaacttc cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct
    21541 agcgttctac ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca
    21601 ttaccaggca gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt
    21661 gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc
    21721 atcacggttg agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata
    21781 atcaatgaca cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt
    21841 tgttggtttc cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag
    21901 gaagtttgtg ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc
    21961 gttaaaattg ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt
    22021 gaccagattg aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa
    22081 agacttgcgg ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat
    22141 gccgggacag ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata
    22201 gctgctgcta gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct
    22261 gaaacaatag ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa
    22321 aatgcaatcc caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt
    22381 gcacacacaa caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg
    22441 accattagag ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa
    22501 ataaaaataa agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc
    22561 caatctgtcg ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag
    22621 atcgtgatat taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata
    22681 accattaata caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc
    22741 tctccttttt tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc
    22801 aattcatccg cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa
    22861 tgactccgcg gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt
    22921 caaatgtatg gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac
    22981 cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt
    23041 accttccata agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt
    23101 atttttataa ctttcacaca caagtttatt ttttactaga aagtagcgcc ctcttgtttt
    23161 tttcttgcta ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc
    23221 aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct
    23281 ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag
    23341 attgctaccg taggctggat tgttggtatg acttggctga catttcctac atcagaagaa
    23401 ccgagacttg ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca
    23461 tgctggaaga ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg
    23521 aacaatccaa actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc
    23581 actttttcat atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca
    23641 gcagcgtaat ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata
    23701 ttgacatctt ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat
    23761 gcttccaaag catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa
    23821 aatttgatgt cgactggatc attcgctaaa gattctgtcg ttttcccata acgaaaagct
    23881 ggatggacgc ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct
    23941 ttagctgatc cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca
    24001 ccagtttcat caatgaattg cttcactatg ctggcggcta atacggagta agtaccaaac
    24061 aaattatgcc cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca
    24121 attgtgggac cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct
    24181 acttcttttt ctacccaaaa cctttctttt tctaattgtt taatcaacgc tgctgaggaa
    24241 taaaactcat agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat
    24301 tctggtaaac gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata
    24361 aaaaattcct cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc
    24421 acgcttgatc cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg
    24481 tctactattt tttgatacgt tttattgttt tcatcttctt ttcttgcaac gatgatattc
    24541 acataaggac gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta
    24601 aatccagcat ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca
    24661 cgtgctgttt gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata
    24721 tcagaaacag tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc
    24781 aataacagaa gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg
    24841 tctttgatat cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca
    24901 gtatttccga tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat
    24961 tgatgttgga aagaattcaa atcaatcgat ccatctgcta aagccacatt tggttgattg
    25021 taatctgtaa atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg
    25081 ttgtcccata catctgtatc ttctccaata atccctagtt ttacgatagt ttcttttttg
    25141 tcagttgtat ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta
    25201 atgactacaa aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt
    25261 tttaatcagt tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt
    25321 agccacaatc gttacatctg tttggaatcg attataccct ctcgatatag caagattccc
    25381 taatccgcct gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt
    25441 caacgccgag acccgaatga taccaggcaa accttctaac aaatacacac gtaaaatgat
    25501 accgattgga ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag
    25561 cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa
    25621 agctgctttc gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga
    25681 aagcaagata atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa
    25741 tagcagacgg ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat
    25801 gattccaatc atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt
    25861 cgcttcgaca aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc
    25921 agtcatttta tcttcctcct tcgatcactt tgatacgact atctaaatcg tttttttgta
    25981 aaatatttat ttttacacca gatttgatca aataatccaa tgcttttttc cgctgtttgg
    26041 tttctccagc taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat aactctacat
    26101 tgccatataa aatattcgct gttacttgat atttgctgta cagatgagcg attagaggtt
    26161 cgttcgtctg atttccaata taacttaatt ccaccagcca tttattggcg atctgatcgg
    26221 caaaagcaga atgggcaatg atcgtttcta atgcttggtc aagatgagta gctgttcgaa
    26281 taaaatcttt ggtcaattct tcttcaggat tgctgaagat ctggatgctg ctaccttttt
    26341 caacgattct tccatcttcc ataaccgcta ccttgttgca gatttctttg acaacttgca
    26401 tctcatgagt gatcaggacg attgttaaac ctaactggcg attcaatttt tttaacaatg
    26461 caaggatctg aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca cataataata
    26521 ccttaggttc attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca
    26581 gttgtttagg ataagcagat gctttttctt ccaaaccgac aagttccagt agttcattca
    26641 ctttttgacg acgttcctgt ttcgattttc cagaatactt taaagaaaaa tcaacgttgt
    26701 caaaaatagt tcggctttcc attagattga aatgctgaaa gatcataccg atggcctttc
    26761 tttcttttcg caattctttg ggagagagct gactcagatt cgtctgattg acaatgactt
    26821 ctcctgctgt tggtttctgc agcaatttca tcacacgaac taacgtactt ttccctgcac
    26881 ctgagtaacc gacgattcca taaacatcgc ctttttcaat cgttaaatct acagaatcta
    26941 ctgcctgtat ctctttttct ttttgtttga aacatacaga aacatctcgc aattcaatca
    27001 ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg
    27061 aggacgcttt tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct
    27121 tcaagtacga tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg
    27181 ctcgggatga attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta
    27241 ccgaagctct ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc
    27301 aaaaaaaaag tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta
    27361 ccaccttttt tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg
    27421 ttcgttttaa cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag
    27481 gccattttca gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt
    27541 ccaaacttac tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga
    27601 ggaaaactga aaagtcaagt acgaaattca aattattttt tctatattta tttttctgtt
    27661 tttttattta acatttcaat aacagttcga agatcttttc cttccgcaag aagtttcaat
    27721 ttttcctttt cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg
    27781 cctaatcctg ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct
    27841 gaactagctt ctaatcctgt tcgttccaaa gctgtcagaa aggaatcggt gaaggcttgt
    27901 tctccgacac ttcgtctccg aatgtaaacg tgtccaagta atcagctaat ttaaagtgat
    27961 ccaagatatc tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac
    28021 gttcttttaa gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg
    28081 tcagctgttg ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc
    28141 tacctgctgc tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt
    28201 caacgatttc ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac
    28261 gatacgtttt ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct
    28321 atacattaat cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaagaaca
    28381 gaagcaaaga accagccttc tcgtttataa gagttcttgg aatcgacgga tatagatttt
    28441 cttcacagct gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta
    28501 attgccagga agtggcatca agcctaattc agcaccaaac cttgtaaaag gaaggacaga
    28561 accgacaata attcctaatg tagtgataac aaacatggca aatgttgcgt tgctttggat
    28621 aaatggtact ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc
    28681 gacaaaccat cctgcatgga ataatgcgat gaaaatcact cgctgtgaac tatccaatgt
    28741 atggaaactt ccgcccacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt
    28801 aatatcaaat atcgaacttg ttggaccaaa gtaaaccatg aattccccaa tactagatgc
    28861 attccatttt tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat
    28921 gcaggaaaca tcatagatca gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa
    28981 tggcaggaac gtacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt
    29041 agccttgata tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc
    29101 caaaatcgtc aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac
    29161 agctgtatct acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc
    29221 atctcctaaa aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt
    29281 ttgctgagga ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg
    29341 atcactcatt tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg
    29401 acggcacact gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc
    29461 atgttctttc agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagc
    29521 aagataacca atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct
    29581 tggattggtc ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac
    29641 ttttgcaagt acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc
    29701 aataaattta gaaatgttca gcatttcttc aatcgcgcct tttgtgatca tttgtgtttt
    29761 accagctgga tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc
    29821 atccactttt tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat
    29881 agccacatct aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg
    29941 caaaacacga tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc
    30001 ttgtgtcaat gtccccgttt tatccgtaca taacacatcg attgcaccaa agttctggat
    30061 ggcattcaaa tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa
    30121 attcgtcgta acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc
    30181 aaaaagaaaa gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg
    30241 agccatcaat gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa
    30301 gcttgattga attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt
    30361 tccaacagaa acaatgattc cttccgcact tccactgatg acattacttc ccataaagac
    30421 aagattttcg taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc
    30481 tactggatag ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa
    30541 taaacgcata tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac
    30601 gatttcttcc ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt
    30661 cacaagtgat ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat
    30721 tgtcccgctg acgataacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc
    30781 aggagctgct aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa
    30841 tgtaaatggc gtgatgtatg ctttcaatat ttctacaatg aatggtgttt ttttgccata
    30901 tgaaatcgca ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc
    30961 atttgctgat gcatggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc
    31021 atagattttt tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga
    31081 cagctgttct cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga
    31141 aaggggctga gaacagatag ataatagccc tggcctttcc tctagatcct tgtttttcga
    31201 atgactgtca tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca
    31261 atcatttttc gaatagttat ttttccaatt agaccacctc ctttttattt ttcaccgatg
    31321 gggatttgcg gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca
    31381 tattaaaaaa agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc
    31441 agatacctgc ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca
    31501 ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca
    31561 aatcttattc ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt
    31621 ttccatgttt ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt
    31681 tatttgtctc attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt
    31741 tatggaaaga acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag
    31801 catcatccaa cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat
    31861 gactcctgaa ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga
    31921 actgatcgat tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt
    31981 gatcgatatc gtccgtaaga tggttggagc aacaaaagct gccgatgctg ttccaggtac
    32041 gatccgagga gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg
    32101 tgatgctgcg gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc
    32161 tgaaaaagcg gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa
    32221 tggccagcag ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca
    32281 ctgtgaagaa ttcttggtag tctttcgtct tttcttcccc taaagcagca gaaatcaatc
    32341 tttgataata atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgcta
    32401 agctccgttt catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat
    32461 aaaacgaagt caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg
    32521 ctaatcgtcg cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga
    32581 atatccaagg aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca
    32641 tcaagtattc ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata
    32701 cttctgtttt ttcgaccatg tgaagattca aaaccaaata atacagatct tctgttttct
    32761 taaaatgcct acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc
    32821 gatctagcat tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc
    32881 attcatattc tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg
    32941 acattttttt cactgaattt gtctcaatcc cgactagaga agtcttaaaa cagattcctt
    33001 ccgctaattg ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat
    33061 catacataat agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt
    33121 aaaaaggaaa aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct
    33181 tcaagcatta gaatgttgtc taactactga aggttggttt gattgtttat tgattatttt
    33241 tttgctgttc aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattaccat
    33301 tttttgaaaa gacacttgtc gaatgttcgc ctttttgctt ttcgattgct tcattacgtt
    33361 ccgcttcatt tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat
    33421 aaaaccgcaa gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt
    33481 tttcctgatc tttggagatt tcctcatcga ttcctgctgc tttcgtatcg atgatttctc
    33541 ctgtctgatt attgtaagct tttcctccta ggacggtgta tttgggtgtc acaaagttcc
    33601 catttcgaaa agcaactact tgatcatgct gagaagataa taagtctgta ccaaaatgaa
    33661 gataatcttt atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc
    33721 caccgtattc atgattgact gtccctttag tataaccagg catatggatc atcaatggca
    33781 ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcgc
    33841 ttgctaaatc tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga
    33901 ttgaattttg gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat
    33961 ccagataatg tgccgttcga acataattat ctactgtgct attgccagta ttcaaagatg
    34021 gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt
    34081 tcgtgtaaaa cggctgttgt agatgctcaa gatattgggc agattctctg aataaatctt
    34141 tatctaatat cccataacct aacgtttcgt cggattcatc aaaataagat cgatcaaaga
    34201 aattgtcata acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc
    34261 catggaaaac agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa
    34321 agacattgtc tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag
    34381 tttccaacat attttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt
    34441 caaagctgat cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc
    34501 cgtctacctt catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat
    34561 ttttcccttt agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat
    34621 aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc
    34681 ctgttttgat tccgtcataa atagtatacg catcgattcc tagatatttg acaatatatg
    34741 aacggtcaaa tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc
    34801 ctgaaaaaat aagccctgaa agacaggtaa cagcgatagc catcggtttg ccgacagggt
    34861 tgcttttgat aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga
    34921 tatccagcca gtagatgaca tcatggggct tcatcaaaga aaaggagctt cctgacaatc
    34981 cttgcgacac tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc
    35041 gataaaaaat aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa
    35101 tagctaacgc tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag
    35161 tcgcaattgg gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa
    35221 aatctgcata gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga
    35281 ttgctaaacg attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt
    35341 cacatttttt cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta
    35401 agattttttt attctttcta aagattattc cgtccacttt tcacagcata aaaccgctgc
    35461 gatgtaagcg attaaactaa tgaagagata aaagtcttgt ggtgaccgct tcactccacg
    35521 gtattcaaaa gccagtttct cggtattcgt acaaaaaaag aggctgtgac aaaattcttg
    35581 tcgcagctcc ttatttcttg atgcaggaca gctttttcac aacttcttcc ttaaacggtg
    35641 ttctcctgcc aactcctcgg ccacaagtca caatatttat aaaacatgag aagctgtttt
    35701 ttgaaattg
//
LOCUS       DACEDM010000027        34662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEDM010000027 DACEDM010000000
VERSION     DACEDM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.027"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(313..2205)
                     /locus_tag="IWO50_001170"
     CDS             complement(313..2205)
                     /locus_tag="IWO50_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358303.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="IWO50_001171"
     CDS             complement(2202..3929)
                     /locus_tag="IWO50_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358304.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="IWO50_001172"
     CDS             complement(3945..4595)
                     /locus_tag="IWO50_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0358305.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="IWO50_001173"
     CDS             4799..5095
                     /locus_tag="IWO50_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0358306.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="IWO50_001174"
     CDS             5287..5907
                     /locus_tag="IWO50_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0358307.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="IWO50_001175"
     CDS             complement(5999..7165)
                     /locus_tag="IWO50_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0358308.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="IWO50_001176"
     CDS             complement(7177..7530)
                     /locus_tag="IWO50_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0358309.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="IWO50_001177"
     CDS             7658..8680
                     /locus_tag="IWO50_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0358310.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9119..9553)
                     /locus_tag="IWO50_001178"
     CDS             complement(9119..9553)
                     /locus_tag="IWO50_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358311.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="IWO50_001179"
     CDS             complement(9663..11522)
                     /locus_tag="IWO50_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0358312.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12800)
                     /locus_tag="IWO50_001180"
     CDS             complement(11640..12800)
                     /locus_tag="IWO50_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0358313.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13107..14531)
                     /gene="araA"
                     /locus_tag="IWO50_001181"
     CDS             complement(13107..14531)
                     /gene="araA"
                     /locus_tag="IWO50_001181"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0358314.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14547..15245)
                     /locus_tag="IWO50_001182"
     CDS             complement(14547..15245)
                     /locus_tag="IWO50_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0358315.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15202..16863)
                     /locus_tag="IWO50_001183"
     CDS             complement(15202..16863)
                     /locus_tag="IWO50_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0358316.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17081..18481
                     /locus_tag="IWO50_001184"
     CDS             17081..18481
                     /locus_tag="IWO50_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0358317.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18733..19812)
                     /locus_tag="IWO50_001185"
     CDS             complement(18733..19812)
                     /locus_tag="IWO50_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0358318.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19898..20857)
                     /locus_tag="IWO50_001186"
     CDS             complement(19898..20857)
                     /locus_tag="IWO50_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0358319.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20869..22392)
                     /locus_tag="IWO50_001187"
     CDS             complement(20869..22392)
                     /locus_tag="IWO50_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0358320.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22402..22965)
                     /locus_tag="IWO50_001188"
     CDS             complement(22402..22965)
                     /locus_tag="IWO50_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0358321.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22971..23834)
                     /locus_tag="IWO50_001189"
     CDS             complement(22971..23834)
                     /locus_tag="IWO50_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0358322.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23848..24744)
                     /locus_tag="IWO50_001190"
     CDS             complement(23848..24744)
                     /locus_tag="IWO50_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0358323.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24919..26190)
                     /locus_tag="IWO50_001191"
     CDS             complement(24919..26190)
                     /locus_tag="IWO50_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0358324.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26180..27697)
                     /locus_tag="IWO50_001192"
     CDS             complement(26180..27697)
                     /locus_tag="IWO50_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0358325.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27895..28785
                     /locus_tag="IWO50_001193"
     CDS             27895..28785
                     /locus_tag="IWO50_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0358326.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28858..29682)
                     /gene="nadE"
                     /locus_tag="IWO50_001194"
     CDS             complement(28858..29682)
                     /gene="nadE"
                     /locus_tag="IWO50_001194"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0358327.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29694..31166)
                     /locus_tag="IWO50_001195"
     CDS             complement(29694..31166)
                     /locus_tag="IWO50_001195"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0358328.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31803..32165)
                     /locus_tag="IWO50_001196"
     CDS             complement(31803..32165)
                     /locus_tag="IWO50_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0358329.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32388..33140
                     /locus_tag="IWO50_001197"
     CDS             32388..33140
                     /locus_tag="IWO50_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0358330.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33280..34203)
                     /locus_tag="IWO50_001198"
     CDS             complement(33280..34203)
                     /locus_tag="IWO50_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0358331.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34218..34490)
                     /locus_tag="IWO50_001199"
     CDS             complement(34218..34490)
                     /locus_tag="IWO50_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358332.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10230 a         7213 c         5979 g        11240 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actatgatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc ctagatgagg caacataagt atacggtcat tttctaagaa ctcttcttct
    12061 ggaaaatcag tcaagtattt agccagatgt tgtttttcca atgcttgcat aacagcatat
    12121 ttatcaacaa tttcacttct tcctacattg ataagtacag catctttttt cattttttct
    12181 atattcgcct gatcgatcaa tccttttgta tcttctgtca acggcagcat gactacgatg
    12241 taatccgagg tagaaagaac tgtttctaaa tctgcttgcc gaacataatc caactgggca
    12301 tcgcggatag aataacccaa gacatccatc cctaagctat agcaacttag cgctactttt
    12361 gtgcctatcg ctccaagacc aagaagaccg atggttttgc cttgcaattc tcgtcccaca
    12421 taagcacttc ttttgttttc tgcctgttct aaaatattag gacctgttag tgtttgtacc
    12481 atacgacttg cttcgattat cggacgggaa gataagagca aacagcacaa aacaagctct
    12541 tttactgcat tagcatttac acctggtgta ttcatgacga tcgtcccgtt ttcagaagct
    12601 ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg aaattgctag caaatctggt
    12661 gtaagccatt catctttcac tttcgtacta cgaataacta ttccttcggc tggcaaatca
    12721 tctattattt tgaaatattc cccttgtaac ttttctatct cttgttctgg aatagcatca
    12781 atcaaatgaa cgtgatacat aaaaccccct ccttactaac aagtatctcg aaatttctgc
    12841 aattagacaa ctgtttacca tttgacttcc tagaagcaaa aagagcagtt agagcagagt
    12901 aggctgagtg ataaaaataa ggctatgaca agttactgcc ataagcctta ttttcgagag
    12961 caaaatattg agggtgagat atcttgataa atggtgttcc gttagctgac attgataata
    13021 agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa tctcttctta tttcttaaaa
    13081 ctaagtggct ttttcacaac ctctttttat ctaataacca ctgtttccaa ctcaactaat
    13141 tttgcccacg tttcaatttg atctgttgtt agatttagcg agacaacggt atgatgaccg
    13201 ccacctgcct gaatccatga atggacacct tcatggaaat tcggtttagt tttccataac
    13261 acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt caaaggcttc tacttcattg
    13321 atcagcagtt tataatgcgt accgaaatct gccatcgata cgactacccc ttctcctgct
    13381 ttcccatcaa atacaagacg cgccggatct tctctatctc ccattgataa aggagaaaca
    13441 acgattcttg gtttcgtcgc agcaagcgtc ggatctactt ccatcatatg ggattccaag
    13501 attgcttctt ggccactagc tagctcgtaa gtataatctt ccatgaatcc tgtatctttc
    13561 cctcgagcca tgattttcag caaccgatca atcgctgcag ttttccagtc gccttcccca
    13621 gcaaaaccgt atccttctgc catcaaacgt tgtacagcta gtccaggtag ttgtttcatt
    13681 ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc ctccttcttc taagaaacgg
    13741 cgaataccga tttcctgctg agcttgtact tttacatgtt cctcccatgt cttttcttca
    13801 taatcaccat agtcgaaggt atacaattcc ttgtactctt caaataattg atcgacttct
    13861 tcgtctgata cgcgatcgat tacttgtaca agatctccga taccaaaata atcaacagtc
    13921 caaccaaatt gaatctgtgc ttccactttg tctccttctg tgacacccac gtttctcatg
    13981 ttgtcaccaa aacgagccac ttttattcca aaactttcat tataggctac tgcaacatcc
    14041 atccaatcag caatctcttt ttggatctca ggtcgtttcc agtagccaac gacgatttta
    14101 ttttgtttat ttaatcgagc attgatgaac ccatattcgc ggtcaccatg agcggattga
    14161 tttaaattca tgaaatccat atcgatcgtt ttccaaggaa tgctttcgtt atattgcgta
    14221 gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc ggatccacat ttttgctggt
    14281 gagaaagtat gcatccaagt gatcacacct gctacttctt ctcgataatt gacctctttc
    14341 atgatttttg tgatcgtatc agcagtaact gctaattctt gaaggcggat tggataaggc
    14401 aattggccat tttcattcag ttcatcgacc atttcttgtg catgtttctt tacttctcgc
    14461 agtgcttctt ccccatataa atgctgtgat cctacaacaa accaaaattc tttttctcca
    14521 atattcaaca taattagcca ctccttttat ttgttttgtc cgtaatacgc attttttccg
    14581 tgttttcgta aataatgttt atctaaaatc cgttgaggca gctcttctga aaatgaattg
    14641 agctgtctgg taaataaatt catcttgcat acttcatcta gtacgactgc gttcattacc
    14701 gcactttgcg catctttccc ccaagtaaat ggaccgtgtc cgtgtaaaag tacccctgga
    14761 attgctaagg gatcaatttt gcgttctttg aatgtttcta tgataacttt tcctgtttca
    14821 tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc gagcacaagg taccgatcca
    14881 taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat ccaatcctgc ttgtgcccag
    14941 atcgtcgccc atgtcgagtg agtatggacg attcctccga tttgaggaaa tgaacgatat
    15001 aaaacagcat gagtaggcgt atccgaagag ggattgagtt taccttcaat gatatgattg
    15061 tccaagtcca caacgaccat gtcgtctgct gtcatctctt cataactgac tccgctaggt
    15121 ttgataacga aatatcgtgt ttcaggatca aaagcgctca catttcccca tgtatatttg
    15181 acgagtccgt gtttaggcaa gtcaagattt gcttggtata cttcttcttt cagttgttcc
    15241 agcattttct atcactcctt tttcttgcaa ttgatgaatc gctgtttttt caatgtcgat
    15301 cccctcacgg taacgtctca taaatatctg atagcctgct atttcttctg ctgaaggtgt
    15361 gatggtgatt ccttggtctt gtccaaacac gtcttctgcc aagaagtttt ctaaagactt
    15421 cccatgtcga tccatcatat aggctgctaa taacgcgatt ccccacgctc caccttctcc
    15481 ggctgtgtcc atcactgtaa ctggtgcctc catagcagat gctagaatcc gctgtccaac
    15541 ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga tcaatttgga cattctcaga
    15601 tttcaatata tccattccga ttctcattgc accaaaagca ctggctaaat ggatccgcat
    15661 gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca aataatggcc ttccttcatt
    15721 tactcctgta atgttttcac cggaaaaata accataagaa agtaatcccc cgcaatccgt
    15781 atcgccttct agcgctttgt tgaataatac cgtgaataat ttatctctgc ttatctccat
    15841 acccaaagaa ttggtaaatt cttcaaaaat actgacccaa gcattgatat ccgaagagca
    15901 gttattcgta tgaaccatgc ctactagttc tcctgaaggt gtagtgacca tgtcgatttc
    15961 cggatggaca ttttctagtt ctttttctaa tacgatcata gcgaaggcgg aagtaccagc
    16021 cgaaatgttc cctgtccctt tggctacact gttcgtagcg accataccgg ttcctgcatc
    16081 cccttctggc ggacaaaccg ggattcctgg tctcaaattt cccgtagggt cgatcagata
    16141 agcccctgat tcgcttaact tgccagcttc ttctcctgct actagaactt taggcaaaat
    16201 gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac tgaccaaaaa tatttttcat
    16261 ttcttgatgg taggttttcg ttgaaggatc gataggaaac atgcctgatg catctcctac
    16321 gcctagtact ttctcaccag taagctgcca atggacataa cctgcaagcg ttgtcaaaaa
    16381 attaatttct ttcacatgtt cttctttatt caaaatagat tgatacaaat gagcgatact
    16441 ccatctctgc ggaatattgt attgaaattt ttccgttaat attcgttctg cttcttcagt
    16501 gattgtattt cgccaagtac gaaatggtac tagtaactgt tccttgctat caaatgctag
    16561 ataaccgtgc atcattgcac tgaacccaat cgacccgatt gtcgtaagat tctcgccata
    16621 gatttcgaat acttctgctg ccatttttcg gtaactgacg cgcagtcctt tccaaatatc
    16681 ttctaatgaa taggtccaga taccattttc tagtttgttt tcccactcat aactaccagc
    16741 ggcaatagga gtaaaagatg aatcaatcaa tacagctttg atcctagttg atccaaactc
    16801 aatccctaaa gatgtacgac catctatgac atctaacata ctttgcgcat ttacttcctt
    16861 cataatgatt taactccctc cttctcgtca aacctgatca aaccgtcttt ttagtagcgc
    16921 ttacaatgat tattgtatac ttttgtacgt acatgtcaag cagttattta caagaataca
    16981 ataaaaaaca cttgttttgt acgtacataa gttataaaat gaaattgctt acaaaatctg
    17041 atcaaccgcc ttctcgaaat tctagaagga gagaatactt atgaaaaaag aaaaaaaagt
    17101 ttcctcatcg tttatttatt tttttggcgc atttggcggc attctatttg gatatgatat
    17161 tggtgttatg accggcgcct tacctttttt acagcatgat tggggtttgg caggtaaagc
    17221 ttctttgata ggatggatca cgtcttcggt catgttaggt gcgatccttg gcggctcttt
    17281 atctggtctt ctttctgata agcttggacg acgaaaaatg attcttcttt ctgcacttat
    17341 ttttatggct ggttctgtcc tttctgcatc tgcccctcac aatggttctt attttctgat
    17401 tgcagcaagg attcttctag gtttagctgt tggcgctgca tctgccctag ttcctgctta
    17461 tatgtctgaa atggctcctg ctagactaag gggaagattg tcagggatca atcaagtgat
    17521 gattgcttct ggcatgctcc tttcctatgt tgcagattat ttgctaaaag ggttgcctga
    17581 aacgatggct tggcgcgtga tgttagggct cgctgctgtc cccgcgctga ttttattttt
    17641 cggtgtttta gctctaccag agtcaccgcg ttttcttatg caatctggcc gcttggaaga
    17701 ggcaaaaaga gtattgaact atattcggac accaaaagaa gctgaacagg aattcgaaca
    17761 aatccagctg aatgtcaaac aagaaaagac aaccggaact tcttggcata ctttgttttt
    17821 agaaaaatat cgttccttag tcattgccgg tattggtgta gcagtattcc agcaatttca
    17881 aggagctaat gctatttttt actatattcc gctgatcgta gaaaaagcga ctgggcatgc
    17941 agcgagcgat gcattgatgt ggccgatcat ccaaggaatc attttagttg ctggttcttt
    18001 gctctttttg gttattgcag acaagttcaa tcggcgaact ttattgaaaa taggcggtag
    18061 cgtgatggga ctttctttca tcctaccagc tgttttagga actgtactgg atgctcatac
    18121 aaattcgttg ttgattctct tgtttttatg tatttatgtc gcattttatt cttgcacatg
    18181 ggctccactg acatgggtaa tcgtaggcga aatattccca ctagctgtcc gcgggcgagc
    18241 atctggccta gcctcttctt ttaattggat tggttctttc ctagttggct tgctttttcc
    18301 tgtgatgact gcttctatgt ctcaagaaat cgttttcggc atttttggga tcatctgctt
    18361 tttaggggtt ttatttattc aagagatcgt accagaaact cgtgggaaat ctttagagga
    18421 aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt atttctatcc atcattctta
    18481 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaaccgct tagtttccag
    18541 tcacaaggaa caatttcaaa aaacagcttc tcatgtttga tgaatattgt gacttgtgac
    18601 cagttggtag gagaacaccg tttagtggag aggtggtgaa agaaacgttc agccttgagc
    18661 gatgacaaaa gctctcacaa acattgcgac gagtaaccgt aggagcaata agaggcggta
    18721 aaaacccgtc tcttattttt cgttttcttt cactgtttcc cgttcaacta gtacaggttc
    18781 atagagaata tttggctgtg cctgtccttt ttcaatcgtt gtgacgatcc accgtgcggc
    18841 atcttgtccc aacttttctt ttggatgcgt caatgttgtc aattttttat tccctaaatg
    18901 gcttaaagaa gaatcgtctg ttcctacgat cgataggtca tctggaatat gatagccatt
    18961 ttctgtcaac ttatctagca ttaaactagc tatttgatcg ttatagcaaa tgatgccatc
    19021 taaagcttgt tcttctttca accgttgtac tgttttttca gcgattgttt ctcgtgtttc
    19081 cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca ttatactgtt cacacgcgct
    19141 gataaaacct ttcatcctgt attttccttg aagatcgtcg atttttgtga tgaacaacag
    19201 acgctgatgt ccttttttta tcaaatattc tgctgccata aaaccgactt tcacatcatc
    19261 taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca ccatcggaat
    19321 gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat tacttttcgt
    19381 aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac actctttctc
    19441 caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt ctcgtaattc
    19501 attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg tggtgatgac
    19561 accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt catctacata
    19621 tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag cgattgcttg
    19681 ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg agggaatggt
    19741 tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg caatatcttg
    19801 atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat aacgagtctg
    19861 caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac ggaacaggtt
    19921 caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat
    19981 taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga tagatcaaaa
    20041 tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca aattgcttat
    20101 ttttttcaga agaaacgaat actggctccg ataatttatg ccatgattct tttaataaaa
    20161 gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct gttgcactac
    20221 cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct tcatttactg
    20281 aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc ttccccttta
    20341 acgtccaagg attatccatt tcggatagat aaagattaga attcccagta atttcaggat
    20401 cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt ccgtctaaac
    20461 tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta ttcattggat
    20521 ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca cgtatagttt
    20581 tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg acttcaggtg
    20641 cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc aggcttgcag
    20701 catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg tagcctggaa
    20761 ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga tctgcccgtt
    20821 gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc acattttctt
    20881 tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt catgtttttc
    20941 tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg tatttttgtc
    21001 caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat tgaaatcatg
    21061 ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag tcagctgatt
    21121 gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt cacaatcata
    21181 aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg ataagtgttg
    21241 catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca gtattaaagg
    21301 tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt gtcgtaaaat
    21361 agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac accaatcgat
    21421 gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta cattccattc
    21481 atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat aatcacaagt
    21541 agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt
    21601 atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact tatcaatata
    21661 gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga tacgatccca
    21721 ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt caattgaatc
    21781 atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt cataagcact
    21841 cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata atttaacgcc
    21901 aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc cttttggaaa
    21961 attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta aattcaccgt
    22021 gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga gtccaaaaga
    22081 atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt ctttcctacc
    22141 tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc gaatgacacc
    22201 taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac catcagctgt
    22261 ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct caacaaatga
    22321 gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt ttactttcac
    22381 ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa tagatacaag
    22441 tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata tgagcagtac
    22501 aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa atactttcca
    22561 tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa tcacatatat
    22621 ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa ttgaaagtac
    22681 gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg ttaacagact
    22741 aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat aaatgatatt
    22801 tcctgatccc tcacctttcc attgcgccat tacataaaaa acagcacatg tagcaggcac
    22861 gatagtaata acaggcaaac acacgattac ccagagaata cctaataaga tgtaatcaga
    22921 aaccacaatc aactgtccat aaagtttagt ctcaactatt ttcatcttcc tcactctttc
    22981 attccagaca tagctgttcc ttgaacaaaa tatttctgtg caaaaatata aaccactagt
    23041 aatggcagca ttgaaataac cgttccagcc attacaggtc cataaaaaga aatgttctgc
    23101 cctgtaaata aagctaaccc tgctggtaaa gttctcattt ttgaactact tgtcaatatc
    23161 aatggataaa gcaagtcatt ccaactattc atcagcgtga aaatccctaa tgataaaaga
    23221 gtaggtttgc tcaatggcaa catgattttc aaaaaaatat taaactcact taatccatca
    23281 atgcgtgctg cctcttccaa agaatctggt aaagtaataa aaaaggaacg catcatgaag
    23341 ataccaaatg cagttgtcat acgaggaatg atcaaacctg cataagtatc aagcatgcca
    23401 agcaaattga cttcaataaa tagaggaatc atgaagactt ggaatgggat catcattgtc
    23461 agcaaaacga aatagaacaa aactgatttt cctttgaaac gcatacgagc aaatgcatac
    23521 cccgcaagag aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa aatcactgta
    23581 tttttgatgt aatctaataa agggatcgtt gtccaaacct taatatagtt atctagggta
    23641 tagctatctg caaatattct aggcggaaat gagataatgt ctttttcgtg tttaaaagaa
    23701 gaaataatca gccacaaaaa aggaaacgca ataaccaaca atataactag catactaatc
    23761 gttcgaatga tttttttacc actaactgct tttcgactat tcacagtaac tgtaatcgag
    23821 ctttctggtg ccatacaggt ttccctccta tttcatttgt ttttctttcc gtgacatgta
    23881 cacattagtg gagatagcta tagttgcaac aatgataaac aaaaccgaag acagtgctga
    23941 tgcatagccc aattcataag gtgctgtaaa tcctcgactg taaatatatt gaacagctgt
    24001 ttctgtctta aattgtgggc caccagcggt tgcaacatat acttggtcaa acacttgcat
    24061 cgaaccaatc agattcgtta aaatacaaaa acctaaggag ggaacgatct gaggtagcgt
    24121 aatgttaaaa aactgctgtc gatttgttgc accatccatt tgagcagatt catacaaaga
    24181 gggagagatt ccctgaatag cagtcaagag aatcgtcata gtaacaccaa aattcttcca
    24241 aacagtcatg atggctaccg tcggcatagc aagggttgga tcgcgaaaaa attgagggat
    24301 cggcaagccg acttgtgaaa acaagtaagg aatcgcccct acattcggat caagcaacat
    24361 agaaaaaata atcgcaattg acgtcaatga acaaataact ggaatgtaaa atgtcgaacg
    24421 acaaaaacga ttgaatctgg tatttttaga taatgctact gctgctaata aaccaacgat
    24481 aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg aattaactgc
    24541 tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc ttgatgtaaa
    24601 gaaaatattc aaatctgtaa aactaatagc aaatgttcct aaaagcggta tgacagtaaa
    24661 taataataaa actaaaaaag atggagttaa aaacaaatac gcagcacgat caggacgatt
    24721 ccaatatttg gtgaatccgt tcatatagtc cacctcatct ttcattttga taaaatatac
    24781 aatcttggtt taaacgaatc tgaataagat atccgaacaa tcgctgtgat ttttgcaata
    24841 acacctatct gcaaatacta ggtcgatcat tcagatatct gtatcttgcc aacagtctat
    24901 cctgtataaa gttctgtact atttttgcag taattgattg atggcatcat ctgcatcgtt
    24961 catgagtgtc tcaggatctt ctcctgctaa taatttttca atcagtggat tgatgatatc
    25021 gttattgatc gcagagattt gggtaaaacc tggtaaatat ggctctgcat agtccatttg
    25081 tttcgataat tcagatacaa taggatcatt ttttacttct tcatcttttg ctactgaatc
    25141 caagtaggca ggaaaaccat tttcgatact ccatttttta ccaatttcag ttgtattcca
    25201 atatttcaaa aactcataga cagcttcttt tttattatta tctgtacttg ccggaatggc
    25261 atatcctacg ccatctaaaa tagctgtttt tttgtctgaa tcactccctt gtggtaaggt
    25321 agtcacatta taatcaatct cgtttgcttt caatccgtta tttaaccatg gcccattgat
    25381 ttcaatggct agttgaccag cattcattaa attgtccatt tcggcgccag aaatcgattc
    25441 tggaccagtc ttgtctacat ggatcatttt ttggatcgct tttaacgttt taaggttctc
    25501 aggagacgca aattcagact tagtataatc atcgttcact aatttaccgc catttgctaa
    25561 catccagtta tataacggtg cagttccatc cttattgaag acaaaacctg atacattttt
    25621 actactatca gttaattttg gtgccatttc tgtcaattct tcccaagtag taggtggttg
    25681 ttcagtatct aatccagctt gtttaaacaa atcattattc caatacatat acatgccttg
    25741 tacctgcatg gggacaaaat actgtttacc ttctacttca cctaattcaa tagccgtttc
    25801 tttaaaatca gatttatcta ctccatcata ttcccagaaa tcgtctaatg gttgtaccgc
    25861 tccgttttct acatactgtg caaaatctgg ataatttaat gcaataaagt caggtgcttt
    25921 tttagaagaa atcgctgtag gaagtttttc attcaaattg gcccaagtca tgatatccat
    25981 ttcaattttg atgtttttat catttgtttt attaaattca ttaacgattt cttgcaatat
    26041 ttcaccatca gatgcagtaa acccattcca aaaagtcaaa gtcacttctt tatcagaatc
    26101 agattctgct gaacttccgc caccgcaagc tcctaaaata cctgttgcag ctaccaacaa
    26161 agagccaatt aaccattttt tacctttcat tttttgttcc tccttcatct gataaacgac
    26221 atctgaccac attccaagat agcggtttca ataaacaatt gattttccca tcttcagctt
    26281 ttacatttgt tgaatgactt ggttttactt gttcactttt aggaagatta gttgcttttg
    26341 tatcaaatcc actcatttca gaaaaatcaa tcactccctc taatgataat tcagacaagt
    26401 gaacagttag attcatttct tcttctccac ggttaactgc aaatatgaca agttctttgt
    26461 catttattac ggctatactt tctaaatacg gcacttcttc aaaatcttcc gtcgcatagt
    26521 ttgctgaacg aatttgtggg cttaacacct ctccttgtcc atacacagca gcttgcataa
    26581 atggataata gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg attggtgcaa
    26641 taacatttac caattgagca agacaagcaa tctttactct atctgcatgt ttcaataaag
    26701 tgattaatag acagcctaat agtaatgcat cttcaaaatt atagttatct tctagtaaag
    26761 gaggagcaat ctgccaaggc gctgcttgct catcttgttc cgctgaatga taccaaatat
    26821 tccattcatc aaaggacagg ttgatctgct tcttactatg tttcttcgcc ttgactgtat
    26881 cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc taaggaacgt gcaagataat
    26941 gagaaagatc atttttttgg ttgccataat attgatgcag agacaaataa tctatttgat
    27001 cgtacgcttg atcaagaact gttaactccc aatcaccaaa tgtaggcatt tgccgattcg
    27061 aacttccgca taaaaccacc tcaattgaat catctacgag tttcatcgcc ttagccgttt
    27121 cctctgctaa ccgtccatat tcttccgctg ttttatgtcc aatctgccaa ggaccgtcca
    27181 tttcattccc taaacaccat gtcttaattc caaaagggct ctcttgacca ttcgaacgcc
    27241 ttaaatcact ccaataagtt cctttaggaa aattacaata ttcgactaaa ttacgagctg
    27301 catctactcc acgagtaccc agattgacag ccatattgat ttcggccccc acatgctgac
    27361 accagttata aaattcatga atcccaactt cattcgtttc aattgtccgc caagctaaat
    27421 ccaaccttct aggtctattt gctatgggcc caatactgtc ttcccaacga aatcctgaca
    27481 caaaatttcc accaggatat ctaatcatgg gaatgttcaa ttcttttacc aagtgaatga
    27541 cgtcttggcg aaatccactt ttattagctg atggatgatc tggctgataa atcccttcat
    27601 atactgctcg tcccatatgt tcaataaatg aactgtacaa ccgcggatca actttgccta
    27661 cacgcttgac actactaatc aacattgtgg cttccataac tgacctcctt taatttatac
    27721 atgaataata cacctcgttg aaagcgttgt caataatata aatgttaaac ttacttattt
    27781 tttatcgatt ttaattaacc aaaatattaa taatgattaa aacaaataat ttaatataac
    27841 ttttattaac aaatatgtta atatataaga aaaacctttt aagaggtgat atatatgcaa
    27901 ctacaaataa acgaagaatc cttaccggta tatgaagcat tagctagtaa aacaaggatc
    27961 aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc aaaagaactt
    28021 ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc aaacatcatc
    28081 aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa aatcgatatc
    28141 agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat acctattggt
    28201 cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca tgattttatc
    28261 gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc ccgcattctc
    28321 tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac agaggataca
    28381 ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc aaatgacaat
    28441 tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg gacgagccct
    28501 ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga taatcttaat
    28561 caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat gaatggagaa
    28621 cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg gcatttgcga
    28681 atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg taaaggattc
    28741 ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt ttcttttcta
    28801 ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc tttattgtta
    28861 tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg tcttgttcca
    28921 ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta gataatcatc
    28981 aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt tcccatcttc
    29041 aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg cttgaagcaa
    29101 ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc caccatcacc
    29161 aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta atacggctcc
    29221 tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt tgatatttcc
    29281 tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt gtgcatcaac
    29341 agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa gagcagcttt
    29401 tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa attgatagtt
    29461 ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac ggcctgctaa
    29521 agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg gatatttttt
    29581 caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat caattgttgg
    29641 tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc ctcctatgct
    29701 tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat tttatggttc
    29761 cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg tttatactct
    29821 tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag tgcaggtaat
    29881 tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc tgtaaagtct
    29941 cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc ttcttcttct
    30001 cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc aaaatttcga
    30061 gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt gcttgataac
    30121 ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt gaatactgca
    30181 ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca tacatcaatt
    30241 ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt cgaagcatag
    30301 atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt agatatatag
    30361 gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc taattctttc
    30421 gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc gactaagaac
    30481 acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt tccataagat
    30541 tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt cccagcacgt
    30601 acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca gactgctgca
    30661 tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc gatgacagat
    30721 ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt caacagtgct
    30781 gtttctacta attggcagtg cgctaaaggc ccttccactt ggattaatgg ttcatttgca
    30841 aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa attttgtaaa
    30901 tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat atcggaatca
    30961 ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa aaccgcgtat
    31021 ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa atcagctcga
    31081 cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg cagcgtcaaa
    31141 ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt tttcatttcc
    31201 tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt ctgaaaaagc
    31261 acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac tatcctttct
    31321 tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat ccgagacacg
    31381 aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt cctactacaa
    31441 tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct acaacataat
    31501 caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct tatccccaaa
    31561 gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta tgacaagata
    31621 taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa gatacaaagt
    31681 aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc agtctcgcct
    31741 tgttttttat tgtatacatt agatatggag caggttacca taaagatgat tattagtcgt
    31801 tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt atttggacgt
    31861 ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac tttgacatct
    31921 tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct tgtgattttt
    31981 ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa tagcagttgt
    32041 gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa aaagaattcc
    32101 actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt ttcgattagc
    32161 ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt tatgttacca
    32221 tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa ggatttacct
    32281 tttatcactt aatagtataa cataatttcg tgagaaaaaa atacaatttt ttcttgacca
    32341 tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg gagtacacaa
    32401 taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt tatgatgaga
    32461 ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat ggcaaaaaag
    32521 aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag ttggatgata
    32581 tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc aagcatcagc
    32641 aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc caacagacac
    32701 ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac
    32761 aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa aaatacggaa
    32821 aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag
    32881 aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag
    32941 aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggttc tcttacagct
    33001 ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac
    33061 ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa
    33121 ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac
    33181 ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc
    33241 cattctttca caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata
    33301 aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca
    33361 agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc
    33421 gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac
    33481 catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg
    33541 ttttcctttc tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa
    33601 aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc
    33661 caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat
    33721 tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa
    33781 aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt
    33841 aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc
    33901 tttatgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat
    33961 gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat
    34021 gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc
    34081 tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat
    34141 tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc
    34201 cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga
    34261 ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc
    34321 caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc
    34381 ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca
    34441 taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct
    34501 ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc
    34561 caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac
    34621 attataatga tactgtaaat atatctttat tattagtgaa tg
//
LOCUS       DACEDM010000028        33818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEDM010000028 DACEDM010000000
VERSION     DACEDM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.028"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IWO50_001200"
     CDS             299..1960
                     /locus_tag="IWO50_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0358333.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IWO50_001201"
     CDS             complement(2030..2377)
                     /locus_tag="IWO50_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0358334.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IWO50_001202"
     CDS             2461..3402
                     /locus_tag="IWO50_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358335.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IWO50_001203"
     CDS             3403..4446
                     /locus_tag="IWO50_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358336.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IWO50_001204"
     CDS             4452..5522
                     /locus_tag="IWO50_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358337.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IWO50_001205"
     CDS             5512..6465
                     /locus_tag="IWO50_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0358338.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IWO50_001206"
     CDS             6477..7487
                     /locus_tag="IWO50_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0358339.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IWO50_001207"
     CDS             complement(7772..8593)
                     /locus_tag="IWO50_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0358340.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IWO50_001208"
     CDS             complement(8690..8968)
                     /locus_tag="IWO50_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358341.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IWO50_001209"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IWO50_001209"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0358342.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IWO50_001210"
     CDS             complement(11724..12311)
                     /locus_tag="IWO50_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358343.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IWO50_001211"
     CDS             12486..13808
                     /locus_tag="IWO50_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0358344.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IWO50_001212"
     CDS             13963..14739
                     /locus_tag="IWO50_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0358345.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IWO50_001213"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IWO50_001213"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0358346.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IWO50_001214"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IWO50_001214"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0358347.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IWO50_001215"
     CDS             16629..17258
                     /locus_tag="IWO50_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358348.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWO50_001216"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWO50_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0358349.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IWO50_001217"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IWO50_001217"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0358350.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IWO50_001218"
     CDS             19445..19981
                     /locus_tag="IWO50_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0358351.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IWO50_001219"
     CDS             complement(20021..21784)
                     /locus_tag="IWO50_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358352.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IWO50_001220"
     CDS             complement(21787..23502)
                     /locus_tag="IWO50_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358353.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IWO50_001221"
     CDS             complement(24281..24505)
                     /locus_tag="IWO50_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358354.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24705..27473
                     /locus_tag="IWO50_001222"
     CDS             24705..27473
                     /locus_tag="IWO50_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0358355.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27555..27974
                     /locus_tag="IWO50_001223"
     CDS             27555..27974
                     /locus_tag="IWO50_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0358356.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27986..28456
                     /locus_tag="IWO50_001224"
     CDS             27986..28456
                     /locus_tag="IWO50_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0358357.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28473..29252
                     /locus_tag="IWO50_001225"
     CDS             28473..29252
                     /locus_tag="IWO50_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0358358.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29239..30060
                     /locus_tag="IWO50_001226"
     CDS             29239..30060
                     /locus_tag="IWO50_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0358359.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30077..31126
                     /locus_tag="IWO50_001227"
     CDS             30077..31126
                     /locus_tag="IWO50_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0358360.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31139..32143
                     /locus_tag="IWO50_001228"
     CDS             31139..32143
                     /locus_tag="IWO50_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0358361.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32776..33795
                     /locus_tag="IWO50_001229"
     CDS             32776..33795
                     /locus_tag="IWO50_001229"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0358362.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11041 a         5992 c         6909 g         9876 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga ggttttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gcttcatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt ttagtatgtg taagtgaaaa
    27541 gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa tttgcttcag
    27601 ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact gtattcgatt
    27661 gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt atgaggtaca
    27721 aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac aacgaattct
    27781 tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg ccactattga
    27841 ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc cagcaggtgc
    27901 taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag aaaatagaag
    27961 aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc atcggttatt
    28021 acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta ttttgattgc
    28081 taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag ccaagccaca
    28141 aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga aaagcggggt
    28201 gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat ataagctggc
    28261 agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa aacctggtac
    28321 tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct tgaaacgatt
    28381 aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa aagtggatgt
    28441 aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc ttattaggaa
    28501 ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt tctcgtccga
    28561 ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg atcattatgg
    28621 gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg attccacctg
    28681 atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga gccggtacag
    28741 aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg aaaaatattt
    28801 acttagggct tttgattcca attatgaacc ataaagcaga caattatgca gaagaaggaa
    28861 attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc ttgatgcttg
    28921 gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat ctattaaata
    28981 tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt ccagctcttg
    29041 gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat tttttcttag
    29101 gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt tttggtgcga
    29161 ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt caaacaactg
    29221 caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat aacgaaaaaa
    29281 gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa ttatgaacgt
    29341 cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt atatcagtcg
    29401 aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac gaccccacat
    29461 atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa ggatgatcct
    29521 gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc tcttgcaggg
    29581 ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt gggtacctct
    29641 ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt caatattccg
    29701 cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac gggttttttg
    29761 aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc tattattgga
    29821 ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc tttaaaaatc
    29881 ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt tccaaatatc
    29941 ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt gaaaccatta
    30001 acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg gattttttaa
    30061 aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa aggaagtact
    30121 aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa aaacagtgaa
    30181 tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga agccagcatt
    30241 ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt tcaattatgg
    30301 aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga aaagtgcttc
    30361 tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg ctatcacaaa
    30421 tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag gtgccggggt
    30481 agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt tactactctt
    30541 agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac aagactacat
    30601 gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa cggaagaatt
    30661 ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca ttggatatgg
    30721 tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa tccgcgttcc
    30781 gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg cagatgcttc
    30841 gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc aaaagttgca
    30901 aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa aagcaagaaa
    30961 cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat tagcgcttgt
    31021 cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg acttgaacaa
    31081 aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg tggaaatcat
    31141 gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag ctttacgtcc
    31201 gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg tctattatct
    31261 aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga atgggaaaag
    31321 caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa acaaaagatt
    31381 aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag aagtagtgaa
    31441 agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg ataaagcgaa
    31501 cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg gaacagaaca
    31561 aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat tcgaagctta
    31621 ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg aagcagagaa
    31681 aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata gtatgcatta
    31741 ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt gttactggga
    31801 agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc acgggacgtt
    31861 agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg aacagagagt
    31921 gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata cattgataga
    31981 cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg ttttgtcttt
    32041 cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt tgaactgcca
    32101 tccattagag atacgccgct actatcgtca gtttgattat taggatagac tggataaaaa
    32161 gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta aaatattggc
    32221 atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta taatctgttt
    32281 aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt agttgttgca
    32341 tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag cgttttgtat
    32401 ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac aatctgtccg
    32461 ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt taaatcaata
    32521 ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca ttgtcaaaac
    32581 tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt agactattgt
    32641 aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac acttaaaaac
    32701 aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat caattattta
    32761 tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc ttggggcact
    32821 gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg aaatgtacct
    32881 gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc tgatttgacg
    32941 atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa cgcagatgct
    33001 attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact tattggtaaa
    33061 atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga tacgcataaa
    33121 cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc aattgttgta
    33181 ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac aattacagct
    33241 gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa tgattatttc
    33301 aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact gaaaaatatc
    33361 attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc aaaagcagca
    33421 atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg tgccaatcct
    33481 ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac aagtgttcat
    33541 tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga agaagttcta
    33601 gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt agagctagct
    33661 caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt gtataatgga
    33721 gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat ggagaatgaa
    33781 ttttctcttc gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DACEDM010000029        32445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEDM010000029 DACEDM010000000
VERSION     DACEDM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.029"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(137..1864)
                     /gene="ptsP"
                     /locus_tag="IWO50_001230"
     CDS             complement(137..1864)
                     /gene="ptsP"
                     /locus_tag="IWO50_001230"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0358363.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(1864..2130)
                     /locus_tag="IWO50_001231"
     CDS             complement(1864..2130)
                     /locus_tag="IWO50_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0358364.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            2609..4843
                     /locus_tag="IWO50_001232"
     CDS             2609..4843
                     /locus_tag="IWO50_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0358365.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            4991..5311
                     /locus_tag="IWO50_001233"
     CDS             4991..5311
                     /locus_tag="IWO50_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0358366.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5516..5739)
                     /locus_tag="IWO50_001234"
                     /pseudo
     CDS             complement(5516..5739)
                     /locus_tag="IWO50_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5937..7517)
                     /locus_tag="IWO50_001235"
     CDS             complement(5937..7517)
                     /locus_tag="IWO50_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0358367.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(7918..9294)
                     /locus_tag="IWO50_001236"
     CDS             complement(7918..9294)
                     /locus_tag="IWO50_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0358368.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(9422..10540)
                     /locus_tag="IWO50_001237"
     CDS             complement(9422..10540)
                     /locus_tag="IWO50_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0358369.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            10682..11116
                     /locus_tag="IWO50_001238"
     CDS             10682..11116
                     /locus_tag="IWO50_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0358370.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(11194..11727)
                     /locus_tag="IWO50_001239"
     CDS             complement(11194..11727)
                     /locus_tag="IWO50_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0358371.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(11821..12999)
                     /gene="mutY"
                     /locus_tag="IWO50_001240"
     CDS             complement(11821..12999)
                     /gene="mutY"
                     /locus_tag="IWO50_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0358372.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(12992..13789)
                     /gene="recX"
                     /locus_tag="IWO50_001241"
     CDS             complement(12992..13789)
                     /gene="recX"
                     /locus_tag="IWO50_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0358373.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            13896..15278
                     /gene="rlmD"
                     /locus_tag="IWO50_001242"
     CDS             13896..15278
                     /gene="rlmD"
                     /locus_tag="IWO50_001242"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0358374.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(15452..16030)
                     /locus_tag="IWO50_001243"
     CDS             complement(15452..16030)
                     /locus_tag="IWO50_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0358375.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            16352..16822
                     /locus_tag="IWO50_001244"
     CDS             16352..16822
                     /locus_tag="IWO50_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0358376.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(16930..18156)
                     /locus_tag="IWO50_001245"
     CDS             complement(16930..18156)
                     /locus_tag="IWO50_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0358377.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(18910..19110)
                     /locus_tag="IWO50_001246"
     CDS             complement(18910..19110)
                     /locus_tag="IWO50_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0358378.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(19244..19852)
                     /locus_tag="IWO50_001247"
     CDS             complement(19244..19852)
                     /locus_tag="IWO50_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0358379.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(19937..20407)
                     /locus_tag="IWO50_001248"
     CDS             complement(19937..20407)
                     /locus_tag="IWO50_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0358380.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(20455..21369)
                     /locus_tag="IWO50_001249"
     CDS             complement(20455..21369)
                     /locus_tag="IWO50_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0358381.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            21515..22318
                     /locus_tag="IWO50_001250"
     CDS             21515..22318
                     /locus_tag="IWO50_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0358382.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            22496..23443
                     /locus_tag="IWO50_001251"
     CDS             22496..23443
                     /locus_tag="IWO50_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0358383.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(23511..23804)
                     /locus_tag="IWO50_001252"
     CDS             complement(23511..23804)
                     /locus_tag="IWO50_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0358384.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(23832..24497)
                     /locus_tag="IWO50_001253"
     CDS             complement(23832..24497)
                     /locus_tag="IWO50_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0358385.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(24637..25269)
                     /locus_tag="IWO50_001254"
     CDS             complement(24637..25269)
                     /locus_tag="IWO50_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0358386.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(25276..26343)
                     /locus_tag="IWO50_001255"
     CDS             complement(25276..26343)
                     /locus_tag="IWO50_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0358387.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(26340..27071)
                     /locus_tag="IWO50_001256"
     CDS             complement(26340..27071)
                     /locus_tag="IWO50_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0358388.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(27240..27719)
                     /gene="greA"
                     /locus_tag="IWO50_001257"
     CDS             complement(27240..27719)
                     /gene="greA"
                     /locus_tag="IWO50_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0358389.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(27855..29030)
                     /gene="mltG"
                     /locus_tag="IWO50_001258"
     CDS             complement(27855..29030)
                     /gene="mltG"
                     /locus_tag="IWO50_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0358390.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            29234..30403
                     /locus_tag="IWO50_001259"
     CDS             29234..30403
                     /locus_tag="IWO50_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0358391.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(30670..32292)
                     /locus_tag="IWO50_001260"
     CDS             complement(30670..32292)
                     /locus_tag="IWO50_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358392.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         9974 a         6414 c         5293 g        10764 t
ORIGIN      
        1 ataacatagc ctacaaactg tcactcaaac ttaagcccta tttttgtcta ttcttactga
       61 caaaaacgtt ttcttaaaaa gaacaaaaaa actggcgtag ctcttttaca agccacacca
      121 gcttttgata cgttgtttat tttacatatt catgaacaag ttcaacgact tcttccatca
      181 tatcgcattc tttcaatgca cgatcagcaa gttcagccat tttcgctgtg tccaagcgtt
      241 tcattaaact acgcgtttta agaatagatg tcgcactcat tgagaactca tctagaccca
      301 tacctactag caatggaaca gctgtttgat ctccagccat ttctccacac atacctgccc
      361 attttccttc agcgtgtgct gcatcaatta catttttgat caaacgtaaa attgatgggt
      421 tatatggttg gtacaagtaa gaaacgcgtt cgttcatacg gtctgctgcc attgtgtatt
      481 gaatcaaatc gttcgtacct acagagaaga aatctacttc ttttgcaaac ttgtctgcta
      541 agactgctgc tgctggaatc tcgatcatga tgccgacttg gatcgtatca gatacttcct
      601 tgccttcact tatcaatttt tgtttttctt cttcaaagat cgcttttgct gcgcggaatt
      661 ctttgagtgt agctaccata gggaacatga tacgtaaatt accatgaaca gatgcacgaa
      721 gcaatgcacg catttgtgta cggaacattt cgtcgccttg ttcagataaa ctgatacgca
      781 atgcacgata tcctaagaat ggattcattt catgaggtaa ttgcagatat ggtagttctt
      841 tatctccacc gatgtccatt gtacgaacaa ctacaggttt tccttccata ccttctaata
      901 cagctttgta agcttcatat tggtcatctt ctgttgggaa gtctggagaa tccatataca
      961 ggaattcagt acggtacaga ccaactgctt ctccaccatt attatggaca ccaactagat
     1021 cttttggtgt tccgatgttt gcagctaatt caaaatgctt gccgtcagct gtgacagttt
     1081 ctgcattttt taatttttcc cattctgctt tttgtgctgc gtaatctgca cctgcttttt
     1141 caaattcagc tttttgttca tctgtaggat cgatgattac atcgccttcg atcccgttga
     1201 cagccaaaat atcgccttct ttgactttag cagtaatttc ttttgtccca acgattgctg
     1261 gaatttctaa agaacgtgcc atgatagcag aatgtgaagt acgtccacca atgtctgtta
     1321 caaacgcttt tacaaaattg cgatctaatt gtgctgtatc acttggagtc aagtcatgtg
     1381 caacaacgac cacttcttcg ttgatcattg atggatttgg taaagtaacg cctaataagt
     1441 gcgctaaaat acgtttagca acgtcacgaa tatctgcagc acgctcttgc atataagcat
     1501 tatcttccat tgcttcgaac ataccgatat acatatccgt tacttctttt agcgctgatt
     1561 cagcatttac gctgttgtct ttaatatttt gcttgatttg acctaccatt tctggatctg
     1621 aaagaaccat caaatgggca tcaaatactt gcgcttctgc ttcacctaag ctttgcgctg
     1681 ctttttcacg aatttgctgc aattcttcag tagatttagc taatgcagca tccaaacgag
     1741 cttcttcagc cgcagtatct tctactgttg ccttactgaa tgttaaatcc ggttgaacta
     1801 gcaggtaagc ttttgcaacg gctacaccgt cactagcggc aatccctttt agcatttcaa
     1861 ccattattca gataagcctt ctttcttcat tgtttcaacg attgctgcca ttgcatcagc
     1921 ttcgtcagca ccttctgcag taatagtaac atcagaacct tgacctacac ctaaagacat
     1981 aacgcccatg attgatttca agtttactga tttaccttta tactctaagt ttacatcaga
     2041 gttaaatttg cttgctgttt gtactaatag tgtagctgga cgagcgtgga tcccagtttc
     2101 tgctactacg tgaaattctt tcttttccat attaatcggt ctcctttaag tgagtgttgt
     2161 ttttatgtga gggttttctt ttttagtaat tcagagaatc ctaacaaatg ccaccctttc
     2221 atctgtaaag attaacattt tttttcgtta gatacaacag ttttcattca atttcagata
     2281 aaagtattca aattattccg gattcttttt catccttttc ttcacatttt ataacaaacg
     2341 tgtttatttc ctttttcttg tcgctttctc ttctcctgac gtttatcatt ctttcctttg
     2401 accctctttg ttttcatatg aaacgaatta cttgcttccc tttttatttt tagtataata
     2461 agagtcaaag gtcaggaatg gtcaatagag atttcacctt ttctacctca taaatctctt
     2521 aaaaaactag gggttattct tttacctcat gaaaaacgag atgtaaaata tggatgtacc
     2581 catttttgtg aaaggacgtg aatgattcat gctttgtcaa aactgcggca agaatgaggc
     2641 aacgattcac ttgtatgcca ctgtcaatgg tcagcggaca caactcgatt actgtcagag
     2701 ctgttatcaa aaaatcaaaa atcaacaaaa tggaggtctg actaatatgg cccaaaatga
     2761 tccatttgga tttggcagtt tggacgatct tttccgttcc atgtctcgcc aaatgcaaca
     2821 gcaaggaaat tatgagcaaa caccaccaac acaatttggc ggaaacaaca acttcaatgg
     2881 tggtcagcca cctcaaggca atacagaagg tttattggga gaatacggaa tcaatatcac
     2941 agaacaagca cgacaaggtg atatcgatcc tgtgatcgga cgcgatgacg aaatcaaacg
     3001 agtcattgaa attttaaatc gccgaaccaa gaacaatcct gtcttgattg gagaaccagg
     3061 tgttggtaaa acagcagttg ttgaaggttt agcacaaaaa atcgtagatg gtgatgtacc
     3121 gcaaaaatta atggacaaag aagtcattcg tttagatgtc gtatctttag tccaaggtac
     3181 tggtatacga ggacaatttg aagaacgtat gcaaaaattg attgaagaga tccgtcaagc
     3241 agaaaatgtg atcctattca tagatgaagt tcacgaaatc gtcggtgctg gttctgctgg
     3301 tgatggaaat atggatgctg gtaatatttt aaaaccagct ttagctcgag gagaattgca
     3361 aatggttggt gctactacct taaatgagta ccgcatcatc gaaaaagacg ctgctttaga
     3421 acgtcgtatg caacctgtac gggtagatga accaactgtc gaagaaacga tcagtatttt
     3481 gaaaggtttg caaaaacgtt atgaagacta tcatcatgta aaatatactg atgaggccat
     3541 cgaagcagcc gcaactttat ccaatcgtta cgtccaagat cgcttcttgc cggataaagc
     3601 catcgatttg ctagatgaaa caggttctaa gaaaaacttg acgatccaaa tcgttgatcc
     3661 taaaacaatc gagaaaaaac tgcaagaagc agaagaacaa aaagttttag cttctcgaga
     3721 agaagatttc gaaaaagcag cttattatcg cgatcagatc aataaacttc aaaaaatgaa
     3781 agaacgtcag ctcactgaag aggaaacacc tgtgattacc gaaaaagata tggaaaaaat
     3841 cgttgaacaa cggacaggta ttccagtggg tgaattaaaa gaaaaagaac agacacaact
     3901 gaaaaactta gcagatgact tgaaagctca tgtcatcgga caagataacg ctgtagatcg
     3961 agtagcgaaa gcgattcgcc gaaaccgtgt tggattaaac aaacagaatc gtccaatcgg
     4021 ctccttctta tttgttggac caactggtgt aggtaaaaca gagcttgcga aacaacttgc
     4081 ctacgaacta ttcggttcac aagattcaat gattcgtttc gatatgtctg aatatatgga
     4141 aaaacatagt gtctctaaat tgattggttc tcctccaggt tatgtcggat acgaagaagc
     4201 tggtcaatta acagaaaaag tacggagaaa tccttacagt cttgtcttgt tagatgaagt
     4261 agaaaaagct catccagatg ttttgcatat gttcttgcaa atcttagatg atggtcgatt
     4321 gacagatgca caaggaagaa cggtcagctt taaagatacg atcatcatta tgacaagtaa
     4381 tgccggaact ggtaaagtag aagcaaacgt tggttttggt gcggcacgcg aaggtgttac
     4441 tcgttctgta ttgaaccaat taaacaacta ctttacacca gaatttctga accgttttga
     4501 cgggatcatc gaattcagtg cattatcaaa agaaaacttg atgacaatcg tcacactaat
     4561 gctagacgat gtcaaccaaa tgctggcagc acagcaactt cacatcgaag ttcctacgaa
     4621 tgtcaaagaa aaattagttg acttaggcta tgatccatct atgggtgctc gtcctcttcg
     4681 acgcacgatc caagaacaaa tcgaagatgg tatcgctgaa ttttatcttg atcacccttc
     4741 tatccatgag ttgaaagcaa aactggataa agatggaaaa atcatcgtta cttctaaacc
     4801 agaacgtttg gctaaagaat cagccgaaga aacggctgaa taaatccagt tttatagcgt
     4861 gagacggtat ttctttgaga aatgccgtct cattcttttt tcagaaaagt caaaatactg
     4921 ttcattttat aaaaaagaaa ttataataag agaaacaagc aattaaaaat acataaaaga
     4981 ggtgagcgcc atgaaattgg taaatgtaac aaacagccat tcccgtcttg tattaaacca
     5041 gcttgaaaac actgatgccc atctagtcaa ggtctacaca gccggaaata ccactattgt
     5101 ctacactgaa gcccctgaac ataatgaaat tctgctgatc aatgacaaac gtaaaatcca
     5161 gccgaaagaa atcgaagatg ctaaaaatta tttcttagat aaaatcaaag acgccgttta
     5221 ccacgaggat gaaatcaaag taatcgaatt ggcaggcctt gttgaaatct cgattccaaa
     5281 acatgaatta ggcgctacat tagccgtgta aacaaatttt gaacataata attaaagagc
     5341 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcgaaag
     5401 cagaagcaac tccttcggaa ataagctgaa attcacgaaa attgcttttc aaatttttgt
     5461 gaattccagc ttatttcttg gagttaaacg cttctgtctc agcctctttt tctttctagc
     5521 tttttgattt ttctcttttc ttttgtactt ttctcttttc attttcagtt ctacgcgtgt
     5581 ctttattttt aactttttcc tctttatgga tgattgatcg tactttttgt gcaatttgct
     5641 cgtttttgtc ccagtctttt acatctaata atcgacataa tcgtaacatc tcttcttttt
     5701 ctgttttttt ctttcacgtc tttgccattc tgtttccatg tgtcacactc tcctttcttt
     5761 aatcatatgt acgcaaaata atttttatat ttttttgatt agacatttct atgattttaa
     5821 tatagtaaaa agagccgtca cggaaaatcc gtcacggctc taaacaaaat atgagaggct
     5881 gtttttgttt ggctattctt attacttgat gcagggcagc ttgtgtcaag acttctttac
     5941 atcaaacttt ttaattttac attgggatat ttatccgcaa accagcgttc tgcaaattgg
     6001 ttctcgaata agaacaacgg ttggtcgaaa cggtcacgag ccaagatatt gcgacttgaa
     6061 ctcatcttct catccaaatc ttctggatcg atccagcgag cgattttgtg tcccattgga
     6121 gacatgatta cttcggcgtt atactcattt aacatacgat attggaatac ttcgaattgt
     6181 agctgaccaa cagctccaat gatatattct tccgttagat atgttttata aagctgaatc
     6241 gctccttctt gaactaattg atagattcct ttgtggaagg atttttgttt catgacattt
     6301 tttgcagtca ctttcataaa cagctctgga gtaaatgaag ggagttcttc ataagcaact
     6361 tttagttttc cttcgaacaa tgtatcccca atctgataat tgcctgtatc atatacaccg
     6421 atgatatcgc ctgctactgc tttttcgacg ttttctctcg aatctgccat aaattgtgtg
     6481 acattgctta atttcagttt tttatttgta cgttccaacc agacatccat tcctcgttcg
     6541 aatgttccag aacagattcg gacaaaagcg attcggtcac gatgggctgg attcatattt
     6601 gcttggatct tgaatacgaa acctgaaaat tcttcctcat aaggacttac catctgatca
     6661 tcttgtgttt tgtgtccata aggagctggt gccaattcaa cgaaagtctc taaaaaagtc
     6721 tgtacaccga agttagtcaa tgcagaacca aaaaatactg gtgtctgatc gccacgagca
     6781 attttttctt gattaaatgc atctccagcc tctttgatta gttctacttc ttctaagact
     6841 tgttcatata aggaattttc agttagagga tgattttcgg gaagcagtcc atcttcatcc
     6901 aattctacta atcgttcatc ctgataattt tccggacgat aaaactctac acgattatgg
     6961 tagatatcat aaagaccttc tagcccttta cccataccga ttggccaatt catcggataa
     7021 gattcgatat ctaatagctc ttctaattct tctaacaatt ccaatggttc acgaccatca
     7081 cgatctagtt tgttgataaa agtaaatatc gggatgccac gtttttttac aacttggaat
     7141 aatttctttg tctgtgcttc gatccctttt gcactatcga ttaccatgac cgcactatcc
     7201 acggccatca aagtacgata agtatcttca gaaaaatctt catgtcctgg tgtatccaag
     7261 atatttactc gtttcccttg ataatcaaac tgcataacag aactagtaac agaaatccca
     7321 cgttgtttct cgatttccat ccagtctgat ttggcaaaat tacctgtttt tttgcctttt
     7381 acggtcccgg cttgacggat agccccacca aacaatagta attgttctgt aatcgttgtt
     7441 ttcccagcat ccggatggga aatgatcgca aaagttctgc gattgtccac ttgttgcttt
     7501 aattttggat tattcataac ctaactccat ttcttccatt caattattta tattttctct
     7561 aggcacagat tgtccgatct cttaaagaaa gacaatttca tatagggctg tgacaaaagc
     7621 cgtgtagcag tctctttttc cgagtaaacg gcgttctaga gacagcctat cagcaataag
     7681 cccacccaag ccaaaaatac gaaaagctgt ttttgcttgg ctgctcttat tacttaatgc
     7741 aggatagctt tttcacaacc tttgtcaact actacaactg aatcagtata gcatggattc
     7801 agcgaatgaa aaagccacca aaccataaga gtcaaatatt tttttgtcag ctgactaaaa
     7861 gaaaagacgt gttgcttgtt tattcaagca acacgtcttt cataaccaag tttccaatta
     7921 tcttcttggc tcgtcatctt cgaattcttt tctgaagtat cttcgttctt cttcaggttc
     7981 cgcttctgtt tcttccggat catgaaaaat gacacggatt ttaaggattc gagaaccttc
     8041 catttcttca gaaaccagcg taatattatc gacatcaaac gataactttt cgccttcatc
     8101 cggaatcatg cctaatgctg taatcaaata accagccatc gtatctacat cactcatatg
     8161 cagatcactg tcaaaagctt cattgaactc atcaatcagc atacgtcctt ggatgatata
     8221 ttcgtgttca tcgatttttt cataaagatt ctcaacttca tctgattcat catcgatttc
     8281 tccgacgatt tcttctaaaa ggtcttctag tgttgctaac ccaacgacgc cgccatattc
     8341 atctaataaa atagccatct gattctgcgt ctttttcatt tcatataaca gatcatcaat
     8401 aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt ttcttgatat ctaaattatc
     8461 aaagccgaat ttatgggcag cttttagtaa attctttgta tgaaggattc cgactacttt
     8521 atctttgtct tcgttataaa cagggattct tgaatagttt tcggataaaa cttcattcac
     8581 attttcttcc acagtgtcgt tgatatcaat cataaatgca tccgttcgtg ggaccatgac
     8641 ttctcgggca actttcgtat caagagaaaa aaccccttgg agcatttcta attcttcatt
     8701 attcaatacg ccttcgtttt caagcatata acgcatttca tctctggtca tttttgaatc
     8761 ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa cttgtggaag cagaaagcag
     8821 ccagacgaag ggcttagcta ctacaccaat caagcgtatc agccctgaag tgaactttgc
     8881 gacttcttct gatttattta aagcaatacg tttagggtac aattctccaa aaacaatcga
     8941 tacataggtc aaaatagcca gcacaatgat atttgcaata ttttttgccg ctgtgccgcc
     9001 acctaatacc ggagctaacc gttccgataa agtattagct agtgaagcac cagacaaaat
     9061 gttgaccaaa gtgattccta cttggattgt cgataggaaa ttatttggat tctgcaatat
     9121 ttttaaaagc ttttgagatt tcacatctcc ttcatcagct ttttgctcga ttcgattctt
     9181 attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa gcattgatca atgtcaaaac
     9241 gatcaataat aaaatctgcg ctaacagcga ctgactctca gggtcagcat tcataatcgc
     9301 attctctcct tataaaatta atttttagtt tacgatagaa aaaaacgtgg cactatagcc
     9361 acgttaaaat ggtatcacaa agcaattttc tttgcaataa aaacaagtgt actaaaacta
     9421 cctacgaggt cgtagcaatt ttcttcttgt tttcattttg cttgtcttct ttgacgattt
     9481 tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa aggaacacct aagaagatac
     9541 caagcaatcc ggcaatattt ccagctacta gaagaacgat aatgatcgtt aatggatgga
     9601 ttttcagtga tttcccaatt acattaggat aaatgatatt cccatccaac tgttggacga
     9661 ttagcacaac cacacaacaa agtaatgctt ttaccggatc attgaagact gttactagaa
     9721 atgcaggtgc tagtcctaga taaggtccta aatatggaat taggtttgtc aatcccgcaa
     9781 tgaccccaaa taagaaagca taatccacac caattaccat atatcctaaa aatgtgaagg
     9841 taccaacgaa taaacattcg attgcttgtc cgctaatgta attagccaat gttttattca
     9901 gttgccccaa taactccaca atttggtttc ttcttttttc tgggaaaaac tgttggatgt
     9961 ttggcactag tttttctccg tctttcaaca tatagaacaa gatgaatggt gctgtaatga
    10021 ttactacggt tgccgaagcg atcgttccaa cgatcgaacc taaactattg gatactccac
    10081 taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa ttgctcaaaa tatttattga
    10141 tatctacttc cttgaacaaa ggcaattcgg ctacctcttt taaccagctt tctacctgtt
    10201 tgatgaaatc aggcatatta gatgccaatg aggaaatttg ctgtactaga cttgggatga
    10261 cactaagtaa aatccaaata attgctgcaa ctaaaaggag cagcacaatt gcgacagccc
    10321 atattctttt gatttttgct ttcatcaaca gcttgacaat aggattcaat aaatagtaaa
    10381 gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa aaatgtcccg atcggttcga
    10441 aaataaagtt tatttctgat gaaatcatga tcaatgcagc tatgatcaat aattcaagcg
    10501 accaaaacat tagtttagac tgtctaagtt tttcaaacaa tagatttcct cctttctttc
    10561 ttactagtat atattatatt cgttaaaaat tcttttattc tttttttatt tttgaaaact
    10621 tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag gtggctagac
    10681 catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg ctgtacggat
    10741 caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa tagacaaaga
    10801 tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc aaggaactgt
    10861 acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga ttctttctga
    10921 atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact ttgctaagaa
    10981 tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt tttacgaaaa
    11041 actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc atatttatat
    11101 gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa
    11161 aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt gcttgtaacg
    11221 gttataccaa atatcaatat agccttccga gaacgggcca tctccattat tgatccagtc
    11281 aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt aattcatcat
    11341 tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt caggaaatac
    11401 cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca aaaaaggtga
    11461 agctagatta caataatagg aaaccccctt ttctctgatc attgctatta cattgaacca
    11521 gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga
    11581 ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg tttttaatac
    11641 catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac
    11701 aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat tttgtgcttt
    11761 gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa tgagctttgc
    11821 ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt tcatttggac
    11881 cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag ttttgttctc
    11941 ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa catgccattt
    12001 cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa caaagagttc
    12061 ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa acagatcgat
    12121 ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg tcacttccat
    12181 cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt caaaataata
    12241 cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg gctttgcttt
    12301 ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg
    12361 gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac ctagatccat
    12421 cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca tcgcttcatc
    12481 aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca atctgctgac
    12541 tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga tacttgcaat
    12601 cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag gcgtttgcgg
    12661 catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt tccgggctct
    12721 tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag catttgctag
    12781 ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca ctgtgtctac
    12841 tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac gattatagcg
    12901 ccaaggcagg tttctttttt cttgttcata ccattgaata aactgatctt gaaattcttt
    12961 tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc ttttccgtaa
    13021 tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc tttactttta
    13081 gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct ccttgtttga
    13141 ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct atcgcttgtt
    13201 ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt ctcaataatt
    13261 ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga tgtgcatttt
    13321 gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca ggtttcaaac
    13381 ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag cgcatattcg
    13441 tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct tttaagcggg
    13501 ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat gttcggattt
    13561 ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa ccgaaatcag
    13621 ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt ccttttaata
    13681 atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca gataaatcta
    13741 cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata aacatcctcc
    13801 tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat tatatgttag
    13861 aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt gtcaaaactg
    13921 gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga atcggttatt
    13981 tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta ctagtgaaaa
    14041 tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa aaaacaagca
    14101 aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt caattacaac
    14161 acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa gcattggaaa
    14221 aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg atggatgagc
    14281 cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag actcaaaaaa
    14341 ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca gactgcttgg
    14401 ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg aataaatttg
    14461 atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc atggttagaa
    14521 tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa aaattcccgc
    14581 aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc
    14641 aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc cacttatggg
    14701 gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct cctcgtgcct
    14761 tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc aaagcattag
    14821 atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg atcggcttaa
    14881 gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa gcaatcgctg
    14941 atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa gtcgggacag
    15001 cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt atcatcgtag
    15061 atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa taccctccaa
    15121 aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg gttaatttaa
    15181 gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag actgcacgtt
    15241 gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt
    15301 ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg ttttatgaat
    15361 actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa aagggttgtg
    15421 acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct atcacttgat
    15481 aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta tatccttttc
    15541 gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt gctttgtcgt
    15601 ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga ttccaagttg
    15661 gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa ttccaagtaa
    15721 tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc tgttcatcta
    15781 aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat gtatttttgg
    15841 tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt aagtttcctg
    15901 ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca tgcgcaaaac
    15961 ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat aacgtactaa
    16021 tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct ttattgtatt
    16081 aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa aaaagtaaga
    16141 tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag aatcaaatca
    16201 tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt aataacaaca
    16261 attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga gccttcatgg
    16321 taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata ttcgtttttg
    16381 ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta gcggaaatcc
    16441 cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct ggatatttgg
    16501 gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt tacttaatca
    16561 caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt tcatttttaa
    16621 ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag caatcgatat
    16681 tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc atcatcgcct
    16741 gacaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc tattgaatta
    16801 ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta acacatagaa
    16861 aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta ttaaccaaac
    16921 tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat aaccataacc
    16981 attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg gtgtatagta
    17041 ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact gatcatatgt
    17101 gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat caccgtttgt
    17161 cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat ttttcaaatt
    17221 aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt ggatcgaaag
    17281 aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc cttcgccttg
    17341 caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta catcaagcat
    17401 gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa tttttctata
    17461 attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta cttcccgctg
    17521 acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag aataggctaa
    17581 acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat ccaaagtaaa
    17641 gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt ccatgccatc
    17701 caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa ttgaatccat
    17761 ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg ttccataacc
    17821 tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt tttgtttcgg
    17881 attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc ctaatccacc
    17941 ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc ttactctctc
    18001 agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg tttggctcgc
    18061 ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa cgaatacccc
    18121 taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt tcacattttc
    18181 taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc ataagaaaac
    18241 tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa attcttaact
    18301 atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc aatcgtttta
    18361 tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc aaataatcgt
    18421 gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc taccgttcag
    18481 attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt aaacccatct
    18541 tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa ctagtgattg
    18601 cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac gattttcttt
    18661 taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac aaatggtctt
    18721 ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa gaaaatgttc
    18781 cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt agccaaaaaa
    18841 tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt gtctttattc
    18901 aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac cttcaacaat
    18961 cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt gaattgctga
    19021 gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc ctttttcatt
    19081 gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa agttcttgca
    19141 agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt tcgctctttt
    19201 attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt gctgcttcga
    19261 aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata taatctggtc
    19321 ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct agaattggtg
    19381 tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct aattttccat
    19441 tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct tttttgaatt
    19501 cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt atttctcctg
    19561 taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg ttagcatgtc
    19621 cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc atatcagata
    19681 ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct gaatttaaat
    19741 tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct tcgtcaatat
    19801 aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc ataaaaaaat
    19861 tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag agaaggaaat
    19921 tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct tcaaaaacct
    19981 cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata acaataattt
    20041 gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac aataccattg
    20101 cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg cattcaataa
    20161 gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc gttacaaaag
    20221 caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt aaatcaaaat
    20281 attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca ccaaaaaaga
    20341 gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac tgtcgatact
    20401 gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta tatttcaata
    20461 actttcatct ttatttaaaa aaattaattt tttttgatcc tttgcctttg tctctgcttg
    20521 gatcgtcaca tgtgtaatac catattttaa ttttaataaa gaaattatac gaggatatat
    20581 cttttcaatc tcgcttagta gctgatcttc aaaatcaata tgtccgtcga aaataattat
    20641 cttttcgcta tactgccaaa tatggacatg atgaacatcc tttaccccat cgattgcttc
    20701 aatttcttgt tccatttttt catagtcgat agcaggtgca ctttgtatca aaatagcggc
    20761 agcttttttg atcaccatta tcgcttctat caatatatat atcgaaatga tcagtgtaat
    20821 aactggatcg atccagtaga tatcgaaaaa acggatcaaa attgctccga taatcacact
    20881 taccgaagac agtgtatcac ttaacaaatg caaataacta gattttatat tcaaactttc
    20941 atctgcttct tgtcgtaata ataatactga gaacaaattc gcgagcaatc caataactgc
    21001 tactgttagc atcaaatgac tatttatctt ttctggtgaa gtgaaccgct tgaatgcttc
    21061 aacggttaaa aacaaggata atatgatcaa tatgaatgag ttgatgaaag cactcaatat
    21121 ttctaaacgc tggtagccat acgtacgttt tttatttgct tcttttcggg ccattttttg
    21181 tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg ttatggaaag catctgaaac
    21241 aagagacaaa ctccctgaca taatccctcc aataaattct gataaagtga tcaccgcatt
    21301 caaaaccact actccaaata gtttttttga tgatctctgt ggctgtgtag gcactattcc
    21361 ttcctccatt ttatttatat tggatagtct ttagacaaga ataacatttt tttgatattt
    21421 caccaatcat tcagctgttt ccttaaagca cttgtatttc acttgatact tcgctagaat
    21481 tgcaagtgat gacactacta gatgaggagt acttatgaca acaaaacaat taattatcag
    21541 ttcctttata cgattcaaag acctaaagga ggcgagaaat gtcccatttt ggaaagccat
    21601 tgtctatctt ttagttttga gtataattat ggctttgcca atctcttttc aagtttttca
    21661 agtcttaaac aacattcaat tggatgggca aaagattgct actcgcattc ctgattttac
    21721 aatccaaaac gggcagcttc aaaccgaaga aaaagaagga ttcatttacc aaacaaactc
    21781 cattatcttc acatttgatc cagagggaaa acgaacagaa caggacatct ctactgattt
    21841 gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa cttatcatag ctcttcctaa
    21901 tacaggcacg acttctgctt tgttgaacaa taaccaatta gaacttccct acaccaatga
    21961 atctttaaaa aacttaacag gtaagcagct ccgttctttt ttaagtgaag catctatacc
    22021 actttggata aaagtcctga catttatatt ttctatttat cctagttttc tgaatctgct
    22081 gatcactttg ctatttacca acattgctgc ttttctctat gcacgctttc gactaacaaa
    22141 agctactttt cttgattgtt taaaaacatt gatctattca atttcattgc ctactataat
    22201 tgcaacaatc ttgatggtct ttcttccttc ttttgacact agcgccttca ttgcttttgc
    22261 aggaatcttt atttttgctc aagctgtcaa aggatgggca aaaatatcga tcaattaaga
    22321 aataaagaca actcgttatt tctacttcaa tagacccttt tagaggttgt gacatttttt
    22381 tgtcacagcc ccttttttat ttgaaaaata tttctttctc aaaaaacaag caaggcgctt
    22441 caaagttctt cttgtatgcg ctacacttag agtaaagaaa ggcgggaaat taactatgtc
    22501 agaaagaatc atttatgcag atcgtccatt tcgtgatgaa tttatcgaag aaatccatac
    22561 tatagcgcct ggttatatct ttaaaacaga attacaacca gaggatctac aaaatgtaga
    22621 gatcagtttg ggctggaact cagactacca agaaaaacta ttagcctctt ctaccttgaa
    22681 atgggtccaa tctatctcag ctggtgttga tcagctaccc cttgagactt tttcaaagaa
    22741 acatatttta ctttcaaatg gtagtggcat ccacagtgaa tcgattacgg agcatgtctt
    22801 aggaatcatc cttgggtatt ctcgtggtct ttttcaagca caaaaggcac agagtgacat
    22861 gaaatggtta ggttcatcgg tccattacca atctatccaa gggaaaaaaa tactaatcat
    22921 tggaactggc catattggaa aaatgattgc aaaaaaatgc aaagcattag acatgaactg
    22981 ctatggaatc aatacatccg gtcatccagc agaaggaatg gaaaagactt tttctttaaa
    23041 tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc aatatcttac ctctaacgga
    23101 ggaaacaact gggctctttg atcagcatat gtttgaacac tttgatccaa aatctgtatt
    23161 catcaatgtt ggccgtggtg cttctgttaa gacacaagat ctggtgcagg ctttaaataa
    23221 ccagcaactt tcctttgcag cattagacgt ttttgaggaa gagcctcttc cacaagatca
    23281 tcctctttgg aaaatggaca atgtactgat tacttcccac attgcaggtt tgacacctga
    23341 ttttcaaaag aaattaatgg ctattttttt aaccaatctg aaaagctact ttgccaccca
    23401 tgagcttcaa accaatcaag taaaattgtc tgcaggatat taatagctga aaacaatatt
    23461 ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc ctctcttttt ttattgtttt
    23521 ttaaagtgat aggttggctc ctccaggttg ggatcataat caacaagtaa atcatatcct
    23581 ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg tcccatccat ttccactttc
    23641 tttacaggat gttctggctg ttcaactgac attccaatcg aaaaaccatc atgaacctct
    23701 gttttgccgt agacttttcc aaaaaaacgt attccgtaag tttctggcag atcaatttct
    23761 gagtcaaacc acttttgtgc ttttggcgtt accgttaatt tcatcccaac aacctacttt
    23821 ctcttttctt tctattgatt catcttatcg tctagaatat ccacatcgcc tgtctctcca
    23881 ataaccagta gattatcatt ttcacgaaca tattcatctg ctgcaggaga agcgataacc
    23941 gtttgatcta ctcttcgtat ggcgactact gttaagtcaa aacgttgcct gaaatttaat
    24001 tcagctaatg ttttgttgaa aaatttagga ttggagactc ggatttctgc taaagaatat
    24061 ttatctgaca actctaggta atctaaaata ttctttgaca ctaaattatg cgcaatcctt
    24121 attcccatgt ctcgctcggg atggacgacg cgatcagctc cgatcttttc aagtactctg
    24181 gcatgatact cattttgggc ttttgctaaa atatttggta cgcccatttc ctttaccatc
    24241 agagtaacta agatactggc ttgaatatct tctccaatag cgacaattac atgatcgaaa
    24301 tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg catttgccac aacagcatga
    24361 gtggctatat tcatatattc attgacccga tcttcattac tatcaatagc cagtacttct
    24421 tgaccagctt cgatcagtgt tcggcagata cttccgccaa atcgtccaag acctacaatt
    24481 gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc ttttctcagt ataacaaagc
    24541 ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag ccaagaaaaa taatagttat
    24601 tttctcagct tttgattatc ctctttatca tcgtttttat ttcaccaaac catgtttgaa
    24661 tgcatagata gcggcttgtg tacggtcttc cacttctagc ttagccaata tattggaaac
    24721 atgtgttttt actgttttca gtgtaataaa caattcgtcc gctatttcct gattactttt
    24781 tccttcagaa atcagcatta aaatttcatt ttcccgattc gttagttctt catgtaatac
    24841 aggctcattt cgccgactca ttttttccat catttttgtt gtcacttctg gttctaatac
    24901 tcgctcgcca cgttgcgttg cccttatagc atcagcaatt tcatgtgcgg tagaggtctt
    24961 caacaaatac cctgctgcac ctgcttcaat ggcaggataa actttttcgt catcaataaa
    25021 actggttacg ataatgattt tagcttctgg ccaatctttt aagattgctt ttgttgattc
    25081 gataccatcc atttcttcca tgactaagtc cattaaaatg acgtccggtc gaagtgtcat
    25141 cgccttctca tagccttgtc gtccattttc agcttctcca attacttcaa tgtcttcttg
    25201 aatagataaa taagaagaga cgcctaagcg caccatttca tggtcatcta ctaataaaac
    25261 ttttatcatc cttatctatg cctccttcat caaagggacc ttgatttcta cacttgtacc
    25321 ttgaccttta aaactaatga tttttactgt tccaccaata ccagctactc gttctttgat
    25381 attgttcaat ccataacttc ccgttttcgt ttcattcata ttaaaccctg ttccgtcgtc
    25441 aataatacgt aacaaaagat tattgtctat tttgtgcaaa tatacctcta attcattggc
    25501 tttagcatgt cttaatgtat ttgataacaa ttcttgaacg attcggaaca agtgatcctc
    25561 aatggaacta gatagtttta catcttctac atcccatttc agcgaaatct ggattttatt
    25621 ttgcaattcc ttcaataact gctcgatacc ttgctttaaa cttttctctt ctaaattgac
    25681 tggacgtaga tgaagaagca gcgcacgcat ctcagactgg gaagcgttga tgatctctgc
    25741 taccattttc aactgcttgc gaaacatttc tggtgtctcg cttttttctg cttgctccgt
    25801 caatgccgac atcatcatca ttgctgcaaa caattgttgc gaaactgaat catgcagctc
    25861 acgggctaat cgatgtcttt ctaattctaa aatttcttct ttggtttgtc catctacata
    25921 ttggggacgg ctcgttacaa tttgtaattc actagatact tcaatcattt tttcttttat
    25981 cttagtgatt tccttatcaa tatctttgat gtataaatcg tccgatgcac ttgggatcgg
    26041 aagaagcaac aatttggatt cataattgcc agaagaaagt gcacgcagct tttcttcgat
    26101 tttcccgtat tttgtctttt gaataagtga taataataga aagacaatta atcctacacc
    26161 taaggaaatg gctaacaaat ggaagataag cggaacatat aataaacgtg cacgcagtaa
    26221 ttctccccac caatgacttt gattgctggc ataaaaataa gtgaaaagtg aaaatagaat
    26281 aataaggaaa gccgcaatcc ccgaataaac agctagcatc gctctggata tttttcccat
    26341 catattcgta tcacctcgac atctccaatc aaggtattag taataatctt caatctacga
    26401 gggttttcat cataatcact gctgtagatt ttgatttgtt catttttcaa agaaaattgc
    26461 tcttcttcaa acaagacatt tcctactaat gtcgcatgct ctaatcggat ggctacgccc
    26521 aaaggtacta agatccgtgt tctgcctatc ccttttctta caatcacaat attatcatct
    26581 tttggcaaaa gcgtattgcc caaatctatg atagtatcac cagaaatgac ggcgatgttg
    26641 atatcgtccc attcatagac gtcgtttcca atacgttggt taccgaataa ttgctgtctt
    26701 ttccgctcat tattatgtgt ttttaattgc tctgtttgga tcatcatgat ctgtttcttt
    26761 cgccaaaaag catttttggt taaatctatc cctgaaatct ctacaccttt cagaccaata
    26821 aataaaacgg caaatacagc catcatccat aaagcaggac tgttgactaa gctaaaaaaa
    26881 atgaccaaac ttcctaatat caattgaaaa ttttgaaatt tagtacgagg atgtttgcgc
    26941 atagctagat atatattgaa aataccgaat atcactaaaa gcaaaagtcc agtattgttt
    27001 acgatttgcc aaacagcaaa taacagcagt agtgcttcaa ctaccacaaa aaaacgccaa
    27061 gaactattca tcgctctttc tccctcctga cacattctca tttcatcata cctatttttt
    27121 taactgttgg ctataggact ttagatgtaa gatcgtttgt tgaaatctaa aatacacaaa
    27181 gagaaaaaga tctagagtgt atcatcagta agatgaactc tctagatctt tcagtcagac
    27241 tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggagt agcaatagtt
    27301 acttcatcat ttttacgttt gccaatcaat gcttgcgcaa taggagaatc attggaaatt
    27361 ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg tgtactcttc ttcttctcca
    27421 tctggtagtt cgatgaatgt aactgtttta ccaattgata cctcatctgc atctacaccg
    27481 tcgttatcga tgatttgagc aaaacgaatc atattttcaa ttgttgtgat tcttccttca
    27541 acaaatgctt gttcatcttt cgctgattca tattcagagt tttcggaaag atctccgaaa
    27601 ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt tgactgtttt tagttcttct
    27661 aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt ctctaccatg
    27721 tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag gaatatagtc
    27781 caaaaaagat taccacgctt ttcttcaaat gtaaatgttt ttctaaaaat gatcaatgtc
    27841 tagagcctgt ttttctaaga attatttaca tatttttcca ctaattccat atgttcttca
    27901 taagttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata ataatagtca
    27961 ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc aaaaggtcca
    28021 ggtccatagc cggtattggt atacaaatta tatggtgaat ctacagctgt atcttcatac
    28081 gtcactaatt ctttgtgttc acctaatgcg tataaaacag agatatctga ctgaagtggc
    28141 atatttgctt tgattcggtt aaagaagact tgtgcaatgt ttttacgatc attttctttt
    28201 acgccttctt tttcaactag agaagctaga gttaaaactt cttgtacagt caaatttttc
    28261 tgcttgatag ctgaataata gttttgcatg accgtattcg tcttgttcac catttcaatc
    28321 actagatcct taagcgaagt actcgtgtaa taatcatatg tggctgggaa taagtaacct
    28381 tccaaacggt attttgtgtt ctcagcttta gatgcacttt ccaacagttc tgggaatttt
    28441 tggtgcaact cgttaaagaa agtctcatca ttcactaaat ctaaaaattc tttcttgtct
    28501 ttaccagtta ccttcgctac ccgttcagca atctgatcga tatcgtaccc ttctggaatg
    28561 gcaattttcc catctgcaac ttttgttggc tcagaagtac cgccttcttg caactgtttg
    28621 ccaatctcgt caagagtcat attaggcgct aactgataat agccagcttg aaagccagtt
    28681 aaatttttaa attttgtata ataattgaaa acaatcccgc ttttaataat attgtccttc
    28741 tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctcgac ttggactaat
    28801 tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata cacggtaaat
    28861 ccaagaaatc cgccaataat caataatgta agagcaatca ctaaaatgat cttcctcaca
    28921 atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc tgtagctgcc
    28981 ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa aacaactcct
    29041 ccttactatc aagttcgtaa ctttattcat tatacatgat atttgaattg gcgtgaatga
    29101 ttttatttaa atattctatc aaaaatgtaa gtagtaaatc cattactata agattaaaat
    29161 aaatgttatc aagatacaag ctattttagc tttattcttt ctgtaaaaaa gaaaggagac
    29221 taaataaact attatgaaaa aaggaaaaca tgctaaaaaa agaaaatatt catttttaat
    29281 gatcgttgtc gtgttgttta gccttagtgc gttctctaca ggaactattc ttggatggca
    29341 attttacaat aatgaacact ctcagatagt tgcccatgct caatcagctc aaaaaaagtt
    29401 gatactgact accaatgaac aacagctcgt tgaaaaaatc gatcaactat taagaaaaaa
    29461 tgattatatt ggctcaattt acgtaaagaa aaataatcgc atcatcatgg aaaaaggcta
    29521 tggctatgcg aataaactca cgcaacaacc aaatgatcct tctctttatt atcagattgg
    29581 ttcggttcaa aaagcattga ctactctttt gattttaaaa caagtcgagc gtgggcacct
    29641 ttctttagat actaagctgg ctcaattcta tccacaaatt gctggcagcc agcaaattac
    29701 tatccgtgac ttgctgtata tgcgctcagg cttgcaccga actgcttctc caaccatacc
    29761 gatgactgat gaagaagtcg ttcaatttgc gattcagcac ttaaaattta cagattatca
    29821 gacctctcat tatgattcat tgaactttac catattgact ggaattttga tcaaattaac
    29881 aaatcaaccg tatgaaaaat tattgaccga tgatatcatc gaacctttag gattacagca
    29941 tacaaaattt tatgaccaag tcaaaaattc atccaatcat gcactatcct atcaaatgtc
    30001 taaagaggat gattacttta aaccactgac agaatctgaa acagagatcc ataacgaatt
    30061 agggactggt aatatcagta tgtccgtcta tgatttaaat acgctgttta cacaagtttt
    30121 atctggaaaa ctagtttcca aagacttact cttctcatta tggcgtgaaa atgcgaatgg
    30181 acatccatat agtggtgggg tatatagcgg agataattat atcctgtcgc aaggaaatat
    30241 cgactctttt gatacatctg ctatatttag caaagattca caggatgcta tcatcatgga
    30301 aagcaatata aaagctgaca aaactatcaa actgcctact gctgatctaa gaaaccaaat
    30361 ctatgacctg atggaaggaa ctactaagtt ggcaaatagc tgatatgatg aaaagatagc
    30421 aaatactact tctttgaata gcatttgctt aaaaaaagta ctcgactaaa cgtaggaaag
    30481 agtgtgggac aaatctaaaa atcaattttt gctacagact ttaattccaa ataaacagaa
    30541 cagaaagagg tgttcttctt tctgttctgt ccaaattatt ctaagatttc tctttaccaa
    30601 cattatttga caaagggcct attaaatcta aaatagtatc tttagattgc taaaatatgt
    30661 gctagcaaat cattcatggc ttgttcttat ttttaatttt atgcaataaa aaagaagaac
    30721 aattagcgtg attatagaaa taaataatga gattttagaa aaaaatgtgt atgaatacct
    30781 gattgttact ttatttattc cactttcact ttttactgta acaaaaggac ctccttgttt
    30841 tagtactgta gcattttttg aattaacagt aaccgttaat cctttatacc ggaatacagg
    30901 taaaactatt gttttgcctt ttgaagattt aaacgtaaat gtaatttcat ctttatttgg
    30961 agaaaagcta ctataaataa cttgaccatc ttctgtaact tgatggtttt tcaaaagcaa
    31021 ttgatctttt ttatagccta taggtacata atcaaaattc tcaaattctt caagtttatt
    31081 ttccatttgt tcattagaat aagttccttg aaaattcatt tctttcacca tgttaactac
    31141 tgatgaataa tttaaaattg aaatagctaa tattaataat agtataaaat ttcttctcct
    31201 tgtataagaa aatttttcca actgtggaga aataaataga gatactgaaa aagatatgaa
    31261 caatgttgaa aatatcataa aacgccaagg agcttgaata atatttaaag gagtattttg
    31321 taaggcaaac catggaaata tttttgtaca aagtagaata gatattatac ccattatata
    31381 agtgatttta gataagattc ctaatttttt aaagttaaat gttagtataa ttaaacaaat
    31441 aagcaaagtt aagccaatac caaatgatag taattctgaa tctatacttc caatcaaaaa
    31501 ctctttaccc agataaattg ctcttttttg taatatatcc acataaggtc tattcagatc
    31561 ttgatacaat gtctgctcaa tcataggacc tataaacgct agtgaaagaa gtaaagtaac
    31621 tatagcagct tttattaatt taataattaa gaagatttct attttctttt tccgataaaa
    31681 ttgaataatt aaagcaatta taatcattac catagtcata gctacagaaa gaacatgagt
    31741 gtaaattaat aaagtcatgc taatcgccaa cataacccaa gattcttttt ttgagtcgta
    31801 aagctttata aacccgagta aaataagagg aagtattgac attgcgagta attcccccat
    31861 tgcccctcga agaaatattt ctacactcct aaaagaagat gttgtatata taatagcgaa
    31921 aaaggagcta gtaacatgtt ttttggtaat ttcatacatt acataatgac atataaatag
    31981 agtaatgagc gtaacaatat aaagaaaaag gttatagcct attgctaaat tagtaaactt
    32041 gatcaataaa aataaaggat atagagttaa ccatgggtaa aaattattta cttgcatgcc
    32101 atatgatcta aacgtttcaa aattaatagg ggagctaaaa atagattcta aacttaacgc
    32161 acgattaatg tgaaatgccg tatcataaaa gccaaatatt tccatgtttt taaatgctgg
    32221 aaataaatat aataaactaa caaatgcata aactataaat atttttacag aaaaaatttt
    32281 tttcaaattc atataccacc tcatgttttt ttatattaca tatagtatca tttatttttt
    32341 taaaacacta gattagtcac tatataaaat gtgattaatt ttattattaa taaaatattt
    32401 tttatattac aaaagtagtg tttcaaagtt agtagagaaa atgta
//
LOCUS       DACEDM010000030        31702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEDM010000030 DACEDM010000000
VERSION     DACEDM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.030"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(718..3867)
                     /locus_tag="IWO50_001261"
     CDS             complement(718..3867)
                     /locus_tag="IWO50_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0358393.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="IWO50_001262"
     CDS             complement(3880..5079)
                     /locus_tag="IWO50_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0358394.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="IWO50_001263"
     CDS             complement(5069..6664)
                     /locus_tag="IWO50_001263"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0358395.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(7248..7955)
                     /locus_tag="IWO50_001264"
     CDS             complement(7248..7955)
                     /locus_tag="IWO50_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358396.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(8020..8856)
                     /locus_tag="IWO50_001265"
     CDS             complement(8020..8856)
                     /locus_tag="IWO50_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0358397.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8874..10283)
                     /locus_tag="IWO50_001266"
     CDS             complement(8874..10283)
                     /locus_tag="IWO50_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0358398.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10297..12120)
                     /locus_tag="IWO50_001267"
     CDS             complement(10297..12120)
                     /locus_tag="IWO50_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0358399.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12963..13289)
                     /locus_tag="IWO50_001268"
     CDS             complement(12963..13289)
                     /locus_tag="IWO50_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358400.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13335..13697)
                     /locus_tag="IWO50_001269"
     CDS             complement(13335..13697)
                     /locus_tag="IWO50_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0358401.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13734..13949)
                     /locus_tag="IWO50_001270"
     CDS             complement(13734..13949)
                     /locus_tag="IWO50_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0358402.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            14076..14411
                     /locus_tag="IWO50_001271"
     CDS             14076..14411
                     /locus_tag="IWO50_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0358403.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14487..16484)
                     /locus_tag="IWO50_001272"
     CDS             complement(14487..16484)
                     /locus_tag="IWO50_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358404.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEIRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16582..16857)
                     /locus_tag="IWO50_001273"
     CDS             complement(16582..16857)
                     /locus_tag="IWO50_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0358405.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            17089..17823
                     /locus_tag="IWO50_001274"
     CDS             17089..17823
                     /locus_tag="IWO50_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0358406.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17862..19004
                     /locus_tag="IWO50_001275"
     CDS             17862..19004
                     /locus_tag="IWO50_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0358407.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(19099..20490)
                     /gene="sufB"
                     /locus_tag="IWO50_001276"
     CDS             complement(19099..20490)
                     /gene="sufB"
                     /locus_tag="IWO50_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0358408.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(20495..20965)
                     /locus_tag="IWO50_001277"
     CDS             complement(20495..20965)
                     /locus_tag="IWO50_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0358409.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(20952..22187)
                     /locus_tag="IWO50_001278"
     CDS             complement(20952..22187)
                     /locus_tag="IWO50_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0358410.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(22184..23470)
                     /gene="sufD"
                     /locus_tag="IWO50_001279"
     CDS             complement(22184..23470)
                     /gene="sufD"
                     /locus_tag="IWO50_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0358411.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(23483..24256)
                     /gene="sufC"
                     /locus_tag="IWO50_001280"
     CDS             complement(23483..24256)
                     /gene="sufC"
                     /locus_tag="IWO50_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0358412.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(24453..25301)
                     /locus_tag="IWO50_001281"
     CDS             complement(24453..25301)
                     /locus_tag="IWO50_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0358413.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(25325..26011)
                     /locus_tag="IWO50_001282"
     CDS             complement(25325..26011)
                     /locus_tag="IWO50_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358414.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(26004..27038)
                     /locus_tag="IWO50_001283"
     CDS             complement(26004..27038)
                     /locus_tag="IWO50_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0358415.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(27433..27771)
                     /locus_tag="IWO50_001284"
     CDS             complement(27433..27771)
                     /locus_tag="IWO50_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0358416.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(27773..28129)
                     /locus_tag="IWO50_001285"
     CDS             complement(27773..28129)
                     /locus_tag="IWO50_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0358417.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(28135..29322)
                     /locus_tag="IWO50_001286"
     CDS             complement(28135..29322)
                     /locus_tag="IWO50_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0358418.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(29513..30358)
                     /locus_tag="IWO50_001287"
     CDS             complement(29513..30358)
                     /locus_tag="IWO50_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0358419.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            30592..31557
                     /locus_tag="IWO50_001288"
     CDS             30592..31557
                     /locus_tag="IWO50_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0358420.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         9184 a         6632 c         5334 g        10552 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
     6721 accccctttt ataaaaagca aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     6781 cgaaaggagt ctaacgttgt ttgatataac ttagactgat cgttaactta ttttttatat
     6841 ttctacatag ttatctttag aaccatttca gatacatatg aaacaattac atatgatttt
     6901 ttcataaaaa aacagtactg tctagtagta taaaatatac aaatttctta aaaatttttt
     6961 cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt aatgaaacta
     7021 tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac
     7081 ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt
     7141 agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg
     7201 ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatcttttta cccattttca
     7261 agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact gatacctgtt
     7321 agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt
     7381 atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc tgtgtatgtt
     7441 tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc tgtctcatta
     7501 ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc tgtctcatta
     7561 ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt agtgtgcata
     7621 gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc aatagagact
     7681 tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag cattttgttg
     7741 tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg gaatacagct
     7801 ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc tccaagtagc
     7861 tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt agaggtcgaa
     7921 acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt agcacaacca
     7981 acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc ttgaaatatg
     8041 cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt cttgaacgta
     8101 ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca ctgaagccag
     8161 caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa taaaaaactg
     8221 taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt tttcgggaaa
     8281 cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca tggtttcttc
     8341 catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc cagcctcctc
     8401 ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc agccgatctc
     8461 aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa aaacaatgcc
     8521 tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa atagactttt
     8581 attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga cgcaaataat
     8641 tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag catcagaaag
     8701 tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc ctttctttag
     8761 ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat ctaagcaaag
     8821 aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc tctctaagct
     8881 aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt tattcgtttt
     8941 aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg cttattgaag
     9001 ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa aacatctaca
     9061 ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc aattcgatcc
     9121 tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc attttcagta
     9181 attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg tagtccagta
     9241 gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc tgcccggtag
     9301 acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac atctgatccg
     9361 tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat aaaatcgggt
     9421 ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa tccacgatca
     9481 ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc taagaaagac
     9541 cagcctctca atgtttccca attatgggct gcaattgcat cttctggact ggaggtagct
     9601 tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt caactgatga
     9661 tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg aaagagttct
     9721 ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg aaggatcatc
     9781 gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc agtaagcaat
     9841 acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc gccctgctcc
     9901 tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat tccctgattc
     9961 aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt tttattattt
    10021 tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct
    10081 ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta
    10141 gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc atcctcctga
    10201 taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt ctctggaaat
    10261 gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca tcaatggttc
    10321 gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac tgttggtcac
    10381 taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat cggcgttgat
    10441 taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa agggttcccc
    10501 ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt ctttagaacg
    10561 tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa caggtgaata
    10621 aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct tagaaaagac
    10681 agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt caacaatgac
    10741 tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc caattagata
    10801 agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt gaacaatatt
    10861 cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa caaaggcata
    10921 tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa tcatggaagc
    10981 aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag taattcccat
    11041 taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt tagttttcgc
    11101 ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc ctggcccatt
    11161 gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca ttgcccaagc
    11221 tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa ttaaccaagc
    11281 tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca acgttccaaa
    11341 cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca tcggcttagt
    11401 aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat aggacatgat
    11461 ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta ataccggaat
    11521 tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa
    11581 aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg ctgccccata
    11641 ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca aaatgatgta
    11701 agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa tggcttttac
    11761 catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa tgacactaat
    11821 caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa ttgaagttcc
    11881 cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca ccacttggta
    11941 ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg ctttcgtatc
    12001 ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg ttaagctgac
    12061 cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc tgtaatccat
    12121 tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg
    12181 aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata acgttatttt
    12241 gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt aattttttat
    12301 cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta gggtatatct
    12361 gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta aaaccattcc
    12421 tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc
    12481 acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag taaaggatgg
    12541 aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt tggtaaaaat
    12601 cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcaata gaatgatttg
    12661 ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc taatctacat
    12721 gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat ccataataat
    12781 tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa attcccgaca
    12841 atcattgtaa tatttttttg tcacctgtat tagtatttaa tttataggaa actataaaaa
    12901 ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat aatacattta
    12961 aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg gtctttcttt
    13021 ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc tttagcccgg
    13081 tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg atttaagttg
    13141 tactgttctt gtaaaatctg tgttaccaat ggttttgatg acttctctaa tccagatgta
    13201 cctgtcattt ttgaaaaaat aatacctata atagccaaga caacaatacc tataatgaca
    13261 aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa aataatttgt
    13321 tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat tgctccagct
    13381 acccctgcta tcatcaaaat cgtacccatt ttctcatctt tttctcgata taagtagccc
    13441 ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgatac taccatagaa
    13501 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
    13561 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
    13621 gtttcttgta gtgctccaca aaactggcaa tacttacttt catctgttat ttctttgcca
    13681 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagttaactg
    13741 tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa tatctcagaa
    13801 agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
    13861 tgctgtgtaa cattcatttt ttttgctaac tccgtttgag aaagtccttt ttcttttctg
    13921 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
    13981 aatatgatgt atttcaactc ataaaatata aaaaacttca aataccatta caaatacaac
    14041 aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa tatgcaaagg
    14101 tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct gaaaaaatct
    14161 tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct aacctcgata
    14221 gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg
    14281 tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact gaagatgcag
    14341 ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat
    14401 tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat
    14461 aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt tctcttcctt
    14521 actctccgtc catcatctct tgttacactt gtaaattgat acagaggaac tatcccagct
    14581 cctgataata tcaattcttt tattgtttca tctatccctg cccctttcgc taaatatatt
    14641 aaaaaactac tacttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca
    14701 agagtgaatc cttgtttaac aaagtagcct ctgtttttta ttaaagcatt tttcacgctg
    14761 ttatccccaa cattacgtag gcgttcacaa acagtgttaa taagttcttt ctctgtcatt
    14821 agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat
    14881 tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt
    14941 tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaattttt
    15001 tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg
    15061 tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt ctcttccttt
    15121 aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc
    15181 aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta
    15241 ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt
    15301 tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgtatt
    15361 tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca
    15421 aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc
    15481 tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact
    15541 ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca
    15601 gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag
    15661 tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca
    15721 ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct
    15781 ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc
    15841 cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta
    15901 atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa
    15961 aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat
    16021 tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc
    16081 ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta
    16141 tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc atctttactc
    16201 aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact
    16261 ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat
    16321 gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct
    16381 aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt
    16441 tcttgatcta cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta
    16501 taaaattctg ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat
    16561 gttttagtct tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga
    16621 ttttttaaat ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata
    16681 ttttctaatc gttcctactg atacacctag atattcactt gcctgctgat agtccatata
    16741 ctctagagtg actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc
    16801 tttcacttgt tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt
    16861 tccccctcct ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc
    16921 tcaaaataaa agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca
    16981 tcgacataca cataatacct cgataaaaaa ggggtgtcaa cagctttgta aaatttattt
    17041 acaacacccc ttttttgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt
    17101 ccaccaataa attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga
    17161 aagagtaaaa aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt
    17221 gacaaaaaaa ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta
    17281 tcccgaattg ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa
    17341 gaatatattt tgggaattat tctttataga gatagcattg tactagatgg tataacattt
    17401 ccagataaaa acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta
    17461 gatacatggc tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac
    17521 aagacttgta ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa
    17581 gaattattca tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc
    17641 agtatagaag aactactaga aagaatggta gatgaatggc tacctattca attgaaagac
    17701 atgtcttatc ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa
    17761 acgatttcat caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc
    17821 tagtggtaag tactccttgt aaactaagag gagatgattt aatggcaaaa atatcaaagt
    17881 acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac
    17941 gaactggtga aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa
    18001 aagattatga cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata
    18061 tgacatttga agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt
    18121 caacaatcgc aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc
    18181 ttaaactatc gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta
    18241 aatatgaaag ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg
    18301 caatgcaata tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt
    18361 atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta
    18421 aagattttgg taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga
    18481 gaaagagtga agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac
    18541 atgtcaataa aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa
    18601 caagagcttc tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact
    18661 ggaaattaca gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc
    18721 aacatgtttt tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt
    18781 taagatatat tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata
    18841 cacatgctag tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag
    18901 ggcatgaaaa tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta
    18961 agaaaacagg tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat
    19021 ctggtcattt ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt
    19081 ctagctccta taacaggttt acccaacact gccttccatc tcataactga tcaatcgatt
    19141 gagttcgact gcatattcca ttggaagttc tttggtgaat ggttcaacaa agcccatgac
    19201 gatcatttca gttgcttcag attcggtcag gcctcggctc atcagatagt agagttgttc
    19261 ttctgagatt tttgagactt tggcttcgtg ttctaaagag acttggctgt tgtggatctc
    19321 attgaatggg atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt cacattcgat
    19381 atgagagatc gaacccgcac tgtttttggc aaaggttact tgtcctcgat agtttacttc
    19441 tccgccgtct tttgaaattg atttagagac gatagagcta gatgtatttg gtgcattgtg
    19501 gatcattttt gcacccgtat cttggatttg gtttgctcct gcaaaggcga tggagagcat
    19561 ggtgcctcgt gcaccttttc catctaagta aacacttggg tatttcatcg ttgtttttgc
    19621 acctaggttt ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg ctcgtttggt
    19681 tactaagttg tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc
    19741 ttttcttgta aagatttcta cgatggcggc atgcaggctg ttacttgaat aagtaggtgc
    19801 cgtacaacct tcgacatagt ggatgcttgc tccttcgtca acaatgatca gcgtacgttc
    19861 gaattgcccc gtgttttccg cattgatccg gaagtacgtt tgtaatggaa catcgacttt
    19921 gacgcctttt ggcacgtaaa taaaggtccc acctgaccat acggctgaat tcagcgctgc
    19981 taatttgttg tcggttggcg gtacaagttt tgaaaagtac tctttgaata attcagggta
    20041 ttctttcaaa gcagagtcag tgtcagtaaa gacgattcct aacttttcga attcatcttt
    20101 catattatgg tacacgactt ctgattcata ttgtgcggaa gcaccggcta gataagctcg
    20161 ttcagcttct ggtataccaa tcttttcaaa tgtatctttg attttttctg gtacgtcttc
    20221 ccagtcacga gcgggtttgt cacttggttt ttggtaatat ttgatggcat cgaaatcgat
    20281 gtctgaaagg tcggggcccc atgtttgcat tggcattttg ttaaatgttt ctagagattt
    20341 cagacggaat tcaagcatcc attctggttc gcctttgatt cttgacatct cacggatcac
    20401 ttcttctgtc aagccttctc ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa
    20461 tttatattct tctaattctg gtacgcccat gttctcactc ctcttccttg tggtaatgat
    20521 ggaaagctgc ggtaccatcg ttttccattg cttttcctag tgctttccag gcaagtgtcg
    20581 cacatttgat ccgagcggga aatttagcta caccaccaag catagcagca tcgcctaatt
    20641 ggtcgggatc tttgacttct tttccttgga caagctgtag aaagtctaca gttaaatgtt
    20701 ctgcttcttc tattgatttt ccgatgacgg cgtctgtcat catcgaggca cttgccgtac
    20761 tgatggagca gccactgccg tcaaaggcga tatttttgat gattccgtct tttacatcga
    20821 cttgcaattc gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt
    20881 cagtcaagct tccacgatga tgcggatggg aagaatgatc caagatcact tgacggtata
    20941 aattgtctaa tttagatagt cccatgttgg aaaaactcct ttgttgccat gattgcttca
    21001 actaaacggt cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct
    21061 gatacttcaa ggtacttcaa tagcggttgc gcacaatgat gacctgctct gacggctacc
    21121 ccttccatgt ctaaagctgt cgcgacatca tggggatgta acccatccaa attgaatgca
    21181 acgactcctg tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc atcaattgct
    21241 tgtaattttg gcaacacata agcggctagt tcttcttcat atcgatggat attgtccata
    21301 ccgagttttt gcaaataatc gattgcatgg cctaatgcga tagcgccggc gatatttggt
    21361 gtccctgctt cgaatttcca aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa
    21421 tcgatcattt cgccaccaaa ttcaacaggt tccatttctt ctaaccattt tcgtttacca
    21481 tataaaacac caatacctgt tggtccgcac attttgtgtc cgctgaaagc aaagaaatca
    21541 cagtctagtg cttggacatc aatggccatg tgcggggcag attgagcacc atctactacg
    21601 atgaccccgc cgttttcatg gacaagttca gctaattctt gaacaggatt gaagacacct
    21661 aagacattgg atacatgagc aatggagacg attttggttt tgtccgtaat ctttttcgct
    21721 gcatcctcca tatccaggaa gccttcttct gttagttcga tatatttcaa gacagcacct
    21781 gttcgttttg ccaactgctg ccacgggata acattagaat gatgttccat gtaagagagc
    21841 actatctcgt ctcctgcttt gatatagcgg tcgccgaagg ttctggctac ccaattcaag
    21901 cttgtcgtcg taccacgagt aaacagcact tctgctgttt cttttgcatg gatgaacgcc
    21961 cgtacttttt ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa cgtatggaca
    22021 ccgcgatgaa cattggcgtt atcgtgttcg taatagtacg ctaatgcctg gagtacttgt
    22081 tttggctttt gggtcgtcgc tgcattatcc agataaacta aaggttcatc attgacgtct
    22141 tgaaataaga taggaaaatc attgcgtagc tttttagcat cgatcatcca ttcaacttcc
    22201 cttcgattac ttcgactaat tctttccgaa cttgttctac agggattgct gttaagacag
    22261 aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc tttatgcaat cctctactca
    22321 tcagatagta catttcttct ggatcaactc gtcctacact ggcagcatgt ccagcagtta
    22381 cttcgttttc atcgatcaaa agaataggat tcgcatcacc acgtgcttta tcagaaagca
    22441 tcaagacacg actttcttgt tgggcatctg caccttttgc ccctttcaag atgtggccga
    22501 taccattaaa ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa atatgcccga
    22561 tcgtatgagg ggctttattg gttactcgtg tatcgattcc ttggatttgt ctgcccgagc
    22621 tgattgccac gattttgact tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt
    22681 cagcgatcac atgaccgtca ttcatgacac caattgccca gtcgacagaa gcatctcgta
    22741 aaatgtgccc acggcgattc atataagaag taatattttc tcctaattga tctacggctg
    22801 aatatttgat ttttgaacca gctttagcga tgacttccac gatgatatta cctgatgatt
    22861 ttgctacttg ttctttggtt gtctggaaac gttccagata agaaaattca ctatgttcat
    22921 ctgccacgat caacacatgc ttgaagaagt gctcatcgga tgcgccatct tgaatgaata
    22981 aggactcgag tggttcatcg atcacgacat ttttaggcac atacaggaaa ataccgctgt
    23041 tcataaaagc tacatggagt gcagtcaatt ggtcttcgtt catgtttact gcttttttca
    23101 tataatattc ttggactagt tctggatatt cgatcattgc agtgaacaag tcggtaaaaa
    23161 tgactccttt ttctgctaat tccacaggca gttgttcaaa aatcgtccat gaaccttgtt
    23221 ggacaagcac aggattgtct ttcattgcat caaatgccgg aatcgtgcct gtttcactga
    23281 tggtttgtgt ttctttcaca tcaaataacg accagcgatg gaatttcacc cgatcgatat
    23341 gcggcaatgg cagttcatct gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat
    23401 caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg agccagtttt
    23461 tttctttcat tcttgctccc ccttacgctt cttcttcttt atagtcgatt cctaattctt
    23521 tactgatacc agcgtagcct tctgcttcca gacgtttagc taggtcagca ttccctgtca
    23581 tgactactcg gccttccatc atgatgtgaa cgacatcagg tgtgatatag ttcaataatc
    23641 gttgatagtg ggtgatgatc aatgcaccga aattgtctcc gcgcatttca tttactcctt
    23701 tagcaacgac tttcaaagca tcgatatcca gacctgaatc gatttcgtct aaaattgcaa
    23761 atgtaggttc taacatcaac aattgcagga tctcattccg tttcttttct ccgccagaaa
    23821 aaccttcgtt cagataacgt tcagccattt cttctggcat atttagtaaa gccatttttt
    23881 catctaactt tttcaaaaat gccataacag aaattttgtc gttttcatca cgtttcgcat
    23941 tgatcgctgc acgcatgaat tccgcattcg tgatacctgg tatctcactt ggatattgca
    24001 tagctaaaaa taagccaagt cgcgcacgtt catctacttc caagtctaaa acatttttcc
    24061 cgtcaaacaa aatttcgcct tctgttactt catagtttgg attgcccatg atagcggcag
    24121 ataaggtaga ttttcctgtg ccgttaggtc ccatgatggc atggatttct ccggttttca
    24181 ttgttaggtt tacgccttta aggatttctt tttcctcgat cgacacatgt agatttttta
    24241 tttctaagac agacatgcat tataccccca aattctcttt cgttctcact cattttagac
    24301 taattgagaa tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt tatttattat
    24361 taaacaattt tgatttctaa attagaatag gtataaaaaa gatgagaaat aagccattac
    24421 agacttattt ctcatctttt ttgttccata ccttattttg catcagcctt tactggttta
    24481 acagagccgc cccatttgtc tgtgatccat tcttggactt ctttgcggtg aagcacttca
    24541 atcagttttt tgattttgtc actgtcttca tcacctttac gagtcgcaat aatattcacg
    24601 taaggagagt tgtccttttc aagcaatacg gcatcttcta atggattcaa ccctgcgccg
    24661 taagcgaagt ttgcgttgat tgcaactagg tcaccttcat cattttggta cgtagaagct
    24721 aaaagtgatg ggtcgatcgt atggttgaac ttcaagttct tagggttttc ttcaatatca
    24781 tcgaatgttg ctgtttccag atctactcca tctttgactt tgactaagcc agcatcttgt
    24841 agaattgtga tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat tgttgcacca
    24901 tcttttaatt cggaaacatc tttgactttt ttagagtaca agcccattgg ttcgatgtgg
    24961 acagcaccgg cattgacaaa gtcatatcct ttttctttga tttctttgtt caaataagga
    25021 atatgctgga agtaattggc atcaatgtcg ccttcttcca aagattggtt cggtaataca
    25081 tagtcatcga atttgacgat ttcaagatcg attccttcat ctttcaataa tggtttcaca
    25141 tgttccaaaa tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct
    25201 gctttatttg ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc
    25261 agcaaaagcg ctgctgcaaa tccaaaaatt ttctttttca tatcctacat ccccctatat
    25321 cctattatct tttgtctgtt ttcttagtta agaaatcacc gattccttga acgataaata
    25381 cgattagtaa aataattaaa gtcgcaacga atgtcactgt tagattacca cgctggtagc
    25441 cttcctgcca agcaagacct ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa
    25501 atccgatcat cgagatcgtt gtaactgtta atccagaaat caatgcggga atactttcag
    25561 gtatcaagac cttccagatg atttcccagt aacttgctcc cattgcatct gctgcttcca
    25621 gtacaccagg gctgacttca cgaaaagcaa tctctactag acgggcataa aatggtgctg
    25681 cggataaaac aagagctgga atggctgctt gcgcacctag catagtgcct acgattgctt
    25741 tagtaaatgg aatgatcaaa accatcagga tcataaatgg cgttgaccgg aaaatattac
    25801 tgatgatcga tacgattcca taaatgattc gagcatacgg tttgtttttg cgtccaatag
    25861 agtaaaggat caatcccaaa actaatccga tgatcgtcac taagaccatt gagattaatg
    25921 tcatatatag tgtatcaagg atagcctgat tcatcgtttc cgggttgatt tgtttaaaat
    25981 ctagatatgt ttctgaaaag cttttatcca cgatggatca cctcaattcc tactccttgt
    26041 tctttgatga acacttctgc ttgatctaat tctttggttt caccagtcat caagacagtc
    26101 aatgaaccaa aggaaccttc tttcacttgt ttgatctttc catacacaac gttgacatcg
    26161 atgttatagc gtcgaatcgc ttgggaaatg actggctcgt tggcatttct ttcgctaaag
    26221 gtcaaagttg ccaaacggcc gcttggattt tctttgatga gttcagctaa tagttctgtc
    26281 gaatcagctt ctggttcgat gtcttgttgt acaaagcgtt tggttacttc ttttttcggt
    26341 tgtctgaaga cagtcaatac gtctccttct tctacgactt tccccaactc catcacagcg
    26401 actttgttgc agattttgcg gatcacattc atttcatgtg tgatcaagac gatggtcaaa
    26461 ttcaatttct tattgatcgc taacaataag tctaatactt cttctgttgt ttgcgggtcc
    26521 aatgcgctag tagcctcatc acaaagcagg atctcaggat catttgccaa tgctcgtgcg
    26581 ataccgacgc gttgtttctg tccgccggaa agttgtgaag gatatgcttg tccgcgtcct
    26641 tctaacccga ctaaacgcaa cagttcttct gcccgttctt tacgtttgct ctttggtacg
    26701 ccagctaatt ccagcggcag ctggatattt tcaagtacag ttcttgacca taacaggttg
    26761 aaatgctgga agatcatacc gattttcttt cggaatgtac gtaattcttt gttttttaat
    26821 tttgtaatat cttgatcgtt tacgatcact tcgccgctcg tgggaagctc aagtccattc
    26881 aggagacgga ctaatgtact ttttcctgcg ccagaatagc cgacgattcc ataaatatcc
    26941 cctttttcaa cgtttagtga aacgtcattg acggcttgga tgtttccgtt tttcccattg
    27001 aatgtctttt tgacattcgt tagttggatt aatgacatgc cattcacatc tttctcttat
    27061 gatttttcaa acaaaaaagc cttcgccctc ttgccttaag caaaaggacg aaagcttgaa
    27121 cttccgtgtt accaccttga tttaccgtta cttcgcaata acagcctctt ccagtacttg
    27181 gacttagctt ctttccgtat actgtcgcgc tgtaacgggc actcccgtag taagtttgct
    27241 tacgctcgct tactctgctc taagaccatc ttccatctca ttttcgttac ccattttcag
    27301 caaacagggc tctcttcaaa cgtccgtgac atgtactctt cttttcaaag ctttctcgtt
    27361 tcatatagga ttgcttatga ttatagcaac tggaagaatc agtgtcaact tttttcaagt
    27421 gattttctga ttttataatt cgtcaaattc ttttggatca acatctttga aacttaagat
    27481 ccacgcattc atttcatctg aatcattcaa gatattgata tcttcaccga ttttatcatt
    27541 aacagaagaa actgtacccg ttagcgggct ggcaaattca ttgaccgctt tttccgcttc
    27601 aacttcgatc agaggttcat ctttttttag cgattgtcct gcttttggca gattggcaaa
    27661 cgtgatgtct ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt attcttttcc
    27721 attaaataag atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca ttttatatca
    27781 gttctccttc ataaattttt tcattgaatc caattgcaac aagctggtcc tctcgtaaga
    27841 tcagtggtcg cttgatcagc ataccatctg tcgataagac ttggctcgct tttgaagccg
    27901 taaactcgcc tacttgatct ttcagtccaa gttcccgata tttcatcccg cttgtgttga
    27961 acaggcgacg gattggtact tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg
    28021 gtacttcttt gatcatatca acggtttgat aactaacgcc atgctggtct aaccacgctt
    28081 tggctttttt gcatgttgaa catttcgggt accagtaaaa ggtgtacaat tttcttacct
    28141 ccttgaagat cttgcaggtg cttcattttg gtatctcatc gataatatca acccgatacc
    28201 gatcatgttc cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg ggatccctgt
    28261 taaaggaagt aatccgatat ttgctccaat attctcaaag acatggaaca gcagcatcat
    28321 gatcaaaccg cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac acacccgaat
    28381 catacgatag attaaaataa aatacaagaa aatgacgaat gcaccaccga taaagccgaa
    28441 gttttcaccg ataacggtaa aaatcatatc ggactcacga acaggcacat agatattgct
    28501 tacgttgaat cctttaccaa acaagccacc ggtaccaatt gccaaaagtc cttgggccgg
    28561 ctgataactc attcctgacg tatcatggaa agggtccagc catgaatcaa tacgtgcaaa
    28621 ctgataagat ttgaatccaa ctttataaag caagtctctt ccgccttcag tcgtaaccaa
    28681 gaagatcgtt cctgctccaa cgactactgc caagattact acaggtgcga tgatgcgcca
    28741 agagattcca gacatcagga aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc
    28801 aaagtcatct tgggccatga tcaatgctaa aaccggtatt gtcacgatca agagcttccc
    28861 aatcagccaa aagtccgatt tcatcgtccg ttcttttact tgcgtattat gtttagttac
    28921 gatcagtgcc atcatcaaga tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa
    28981 cgcaccgaat cgaaaccagt ttttagaccc tgttgaagca gctaaattct gatcatagaa
    29041 tttgagcaag agtcccatta ccactaaccc caaaccataa aggtaaggag tcagtttcca
    29101 gatccatttg gaattcaaat gcatgatgac aacgattgct gccgctccaa ctaaatacca
    29161 gacaacttgc atccctacgc ctttcaagac tgctggattg ttcggatcat ggctaagtgc
    29221 tacatataat gagagcagac caatcaaaca taacagaaag actggtaaaa tcacgccata
    29281 atcaatacga ttatcgttcg aagcttttaa ttttttttcc atgtttcatc ccttttctta
    29341 aaaaccatac tgctgattat tatagcggtc cagttagata atggaaagtc tttccataga
    29401 catttgggaa aaattaactt tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc
    29461 ctactgctta tctttagtgt atcatagaac tttttttgat acatgcaatt atctaccagc
    29521 tgagattctc ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg atctcttcgg
    29581 caatctctag cagctgatca gaaaccagat tggttcgttg gatgaaataa aaatatcgat
    29641 ggaaattcaa cggagtcatt ggcacatttt ctggaacaga gcgcttagac aaaatcgagc
    29701 gaccgattcc ttccttcagc aacgcaacaa ttacatcgtt acttttcacg atcatcttct
    29761 tatcttggat attcatctct tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg
    29821 cttctcttac aagccacaat ttgctttttg ggtccccagc gatgactagc tggtcttcca
    29881 tcaaagagta tctttgtacg ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg
    29941 attcatgttt cgctacttgt tctaatgatt catgagaatt cgtgatttcg atctcaaaat
    30001 gcactttagg aaaccttttg atcagatgtt tcatcaactg aggcaaaaga tagacagcaa
    30061 aggtgtggga agcaacgatc cgacatactt cttcttcatt ttgttcattg agcaattttt
    30121 gctgagtttc ttcccattct tctaacaaaa ccagtgtccg ttcatataat aagtcggctt
    30181 gcttagttgg aagcatctct tttcttccgt tacgttgaaa aagatcgata ttcaattccc
    30241 gctccaactg tttgatttga ttcgagactg ctggttgaga taaaaacagt acttctgctg
    30301 ctctggaaaa atttcttgtt tcatatactg ctcgaaaagt cgttaataac ttaaacatgg
    30361 caccacttcc ataacttttt atgataatta ctataaaaac tatttgattt aaatatacaa
    30421 aaaaccatct ataatagaaa tgtacaacaa aacagttgct gatccaacta gtaacttaga
    30481 tgaagacaat cagctgtctg cttgctcgta atcatacatt cctattcatt ttacaatgta
    30541 ttcgttcatc ttggtactaa aatctattaa ataaaaagga gaatattata tatgaagaac
    30601 tttttgaaaa ttattccgat cccgatctgc gggttgatcc ttgggttagc ttcccttgga
    30661 aatctgttta aagactatca ccatgttgct ttaggcaatc taactggcgg tatctctatg
    30721 attcttatga tattcatttt tgttaaattg tttatcttat tcgaacacac gaaacaaacg
    30781 ttgcatgatc caatcatcgc ctccgtttca ccaactttta cgatgtcact catggttatt
    30841 tgtacttatt ttgtttcata tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc
    30901 gctgttatct tccaagtgat ccttgtcttt tatttcaatt atcaccatgt tatcaaagct
    30961 gatctttcaa ttgaagcaat ttatccaagc tggtttatca tttacgttgg atttggggtc
    31021 atcactgtaa ctgccgggaa cttcttccca cttattggaa aaattttctt ttggatttca
    31081 ttgatctgct atgcgttctt attgccgatc atcatcaaac gtgtcttttt cgtaagaaat
    31141 atggctcatc cgacacgtcc gctgattacg atcgttgcag cacctggttc tctttgccta
    31201 acaggttatc ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc attgttcgct
    31261 ttatctcaag tattgtattt cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt
    31321 ttttatccaa gctatgcagc ctttacattc ccgcttgtta tctctgcaac agctatattt
    31381 actactgttc attacttcaa cagtttaggt atctatagta gcgtattgga tttcttaaaa
    31441 gttgcggaat gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt
    31501 ttcttaatta aagaacatag caaacatgga aaatttgttt ctaaaaaaat cgcttaataa
    31561 tcgattcaat aggttgtgaa aaagctgttc tgcatcaagt aataagaacg gccaaacaaa
    31621 aacagctgtt catgtttttg tactggttgg gcttattacc gcagatgcaa gcttttgaac
    31681 accgtttacc aagaggtcgt aa
//
LOCUS       DACEDM010000031        31574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEDM010000031 DACEDM010000000
VERSION     DACEDM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.031"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(110..577)
                     /locus_tag="IWO50_001289"
     CDS             complement(110..577)
                     /locus_tag="IWO50_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0358421.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(697..2235)
                     /locus_tag="IWO50_001290"
     CDS             complement(697..2235)
                     /locus_tag="IWO50_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0358422.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2315..3001
                     /locus_tag="IWO50_001291"
     CDS             2315..3001
                     /locus_tag="IWO50_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0358423.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3202..3729)
                     /gene="msrA"
                     /locus_tag="IWO50_001292"
     CDS             complement(3202..3729)
                     /gene="msrA"
                     /locus_tag="IWO50_001292"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0358424.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3813..4490)
                     /gene="pgmB"
                     /locus_tag="IWO50_001293"
     CDS             complement(3813..4490)
                     /gene="pgmB"
                     /locus_tag="IWO50_001293"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0358425.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4487..5617)
                     /locus_tag="IWO50_001294"
     CDS             complement(4487..5617)
                     /locus_tag="IWO50_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0358426.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5617..7800)
                     /locus_tag="IWO50_001295"
     CDS             complement(5617..7800)
                     /locus_tag="IWO50_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0358427.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7797..8822)
                     /locus_tag="IWO50_001296"
     CDS             complement(7797..8822)
                     /locus_tag="IWO50_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0358428.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8867..9694)
                     /locus_tag="IWO50_001297"
     CDS             complement(8867..9694)
                     /locus_tag="IWO50_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0358429.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9691..10758)
                     /locus_tag="IWO50_001298"
     CDS             complement(9691..10758)
                     /locus_tag="IWO50_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0358430.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10784..11620)
                     /locus_tag="IWO50_001299"
     CDS             complement(10784..11620)
                     /locus_tag="IWO50_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0358431.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11617..12486)
                     /locus_tag="IWO50_001300"
     CDS             complement(11617..12486)
                     /locus_tag="IWO50_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0358432.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12556..13839)
                     /locus_tag="IWO50_001301"
     CDS             complement(12556..13839)
                     /locus_tag="IWO50_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0358433.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13853..15538)
                     /locus_tag="IWO50_001302"
     CDS             complement(13853..15538)
                     /locus_tag="IWO50_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0358434.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15813..16838
                     /locus_tag="IWO50_001303"
     CDS             15813..16838
                     /locus_tag="IWO50_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0358435.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17433..21086)
                     /gene="rpoC"
                     /locus_tag="IWO50_001304"
     CDS             complement(17433..21086)
                     /gene="rpoC"
                     /locus_tag="IWO50_001304"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0358436.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21165..24782)
                     /gene="rpoB"
                     /locus_tag="IWO50_001305"
     CDS             complement(21165..24782)
                     /gene="rpoB"
                     /locus_tag="IWO50_001305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0358437.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25510..26478
                     /locus_tag="IWO50_001306"
     CDS             25510..26478
                     /locus_tag="IWO50_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0358438.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26496..27401
                     /locus_tag="IWO50_001307"
     CDS             26496..27401
                     /locus_tag="IWO50_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358439.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27398..28204
                     /locus_tag="IWO50_001308"
     CDS             27398..28204
                     /locus_tag="IWO50_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0358440.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28361..29329
                     /locus_tag="IWO50_001309"
     CDS             28361..29329
                     /locus_tag="IWO50_001309"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0358441.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29646..30170)
                     /locus_tag="IWO50_001310"
     CDS             complement(29646..30170)
                     /locus_tag="IWO50_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0358442.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30457..>31574
                     /gene="scm"
                     /locus_tag="IWO50_001311"
     CDS             30457..>31574
                     /gene="scm"
                     /locus_tag="IWO50_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0358443.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDT"
BASE COUNT         8866 a         6819 c         5945 g         9944 t
ORIGIN      
        1 ctcaaaaaac ggcaaaatat gaggaactat tttgacgttt gtttctctaa tactcaggtc
       61 aaaacgctga tttcacaacc tctttttcgg tcaagttcaa caacagtttt tacgcgtcga
      121 tctctggcgc tttgcccaaa tgagcttgga tcatccagat acgtttttct gtggctgttt
      181 tgaattcgat aaagatatct tgtgttggat cgtcgccttc ttctccagaa acttcgattc
      241 ctttttggta aagatctgct agataacgat agcctgcaac aagtgtttcc aaacgttctg
      301 gaatagtacg atcccagcgg ccaatttcat cagggatttt tgtgttatct gcaaattcac
      361 gcaatgtaga ataaggtgaa ccgtctaatg tgatcaagcg ttcggatact tcatctaact
      421 gagcgttcaa atcatccatg aactcatcca tcattgggtg caatgtaagg aaacctggtc
      481 ctctcatgta ccaatgtgtt tggtgaacaa caacagacat ttggcttaga tctgcaacta
      541 attgatttaa tacttctttg gtttgttgga atttcatctt tcttgcctcc taacattttc
      601 ttacacttaa aataatagta gactttcaat gaaaagtaca acaaaatcat ttacctgaga
      661 acgattttca gttcgctttt ttatccgtaa agccaatcat ttttcagaat cagctaattc
      721 ttctcgcagt tgagcaaatt gctgataagt ctgtgctaaa tgcttttgtg tcgaaacaaa
      781 taatggatag attttctgat attttttatg atcagtttct tgtactccaa cttgctcttt
      841 caattctatc aattcctgtg cttcactgat actttggatc tttccgatgc ttctccagcc
      901 taaaatcaca gctcctaatg cggatgcttc tgtgacagta gggaaaacaa gcgggtaacc
      961 cagtacatca gccatcatct gacgaaaaat cggagattga gcaaacccgc ctgtagcacg
     1021 gatctctgtg agctgatttt cctggcaaac tccttttaaa atccgataaa ggttgagaca
     1081 gatcccttct agaatcgcct tcatcatttc tttttctgta tgttgccgct gtaaaccgat
     1141 aaaactgcca aacgcatctg cgtcccaaag cggtgctcgc tcaccaagta aatagggatg
     1201 aaaaatcaga ccattggcac ccgctggcac aaaagcgata gccgaaagca gtgcatcata
     1261 tggattttct cctttttctt tagcagattg aacgacttcc tgtaaaaatg tatgccctgc
     1321 ccaatcaaag atgccagcac cattgctagt cgctcctccc actacccaat gtttctgatc
     1381 cagaacataa caaaacgtct ccatttctgg atggaactga ggctgagtag taataaaacg
     1441 aagtgctccg ctcgttccta cggtcaaagc ggcgatcgta tcaccaaaag cacctaaccc
     1501 tagattagaa agcggcccgt ctgcccctcc caaaacaaat gatatatcat ttggcaaacc
     1561 tagttctgct gctgtttttg atggtagacc tgttacttga taggtggaag gaaagacttg
     1621 cggcaattgc tctattcgaa tgttcaagta gtcaagcact tcttctgccc attggaattc
     1681 atggatatca aaatatccag ttcctgaagc tgttccgtag tcgctgatca gttcgccaaa
     1741 caatctataa aagatatagt ctttgatacc gacaaaataa gcagtttttt tgaataattc
     1801 aggttggctg ttttgcagcc acttgatttt gttcaatggt gtcattggat gaatcggcgt
     1861 cccagtctgc cgataaaatt ctttgccaag cacggtatat ttcagttttt ctgcttcgtc
     1921 cgctgctcgg ttatctgccc aagtgatcat tcgagataac ggtgttttct cttcatccaa
     1981 caatatcagg ccatgcatcg cagaggagaa ggagacaaat tgaatgattg tgtctgaagg
     2041 caactgttta attacttctt ttataacggt gcagacacct tgaaagatca attcctgatc
     2101 ttgttctgcc atcccttgtg tttcttggat caaagggtag cgaaaataag cggatgctgc
     2161 ttcttgggct tcatctgtga aagcaactgc tttggtttgt gtggttccga tatctaatcc
     2221 gattgaaacg ttcatttttt cacctcaaat tttatgtatg tacaaaaatt tagtttaagc
     2281 ttgcgtatct tggttttgaa aagggggaga tggcgtgtta ctttatttta tcattggttg
     2341 ctgtatcgga tcatttttat gtttaaccgc ccagcggatt ccgttaggtc actctattat
     2401 ttatcctaga tctcattgtg tcaaatgctg tcgttcgctt tcttggtatg aattgattcc
     2461 gattctctcg atcatcgtcc aacgatttcg ctgccgctat tgcaggtgtc ggttgccctt
     2521 ctactatttg ttggcagaag ccttatgtgg tggtcttttt gcctggttct ttactttttc
     2581 ttccgcaaga aatttttcga cgttcatatg gatgctttcg gctttgctat tttcgttgat
     2641 ggatttattt tacttcatgg tccattctca cacgctattc tgttcttggg caattttatg
     2701 gattttttgg ttgcaggccg gcgtcttcca atggcaaagt gtgctgttaa cgtttatcgt
     2761 aggcggtgtg tgccttcgct atggtcaatc ctatctcgga gctggcgata ctctgttgat
     2821 gctttcttgg agtggtggct tgtctctaga agagttgctt cagatcttgt ttcttgctag
     2881 tctgtttgga atatccgttt tttctgttta ctttatctgt cataaaaaaa agttggaaaa
     2941 gctgcctttc attccttttt tgagcagtgc gttgctgatt gtccttcatc ttaggtattg
     3001 aaaaaaggtt gtgacaaacg acttgtcaca acctctgata tatagtgttc tcttaccaaa
     3061 tccttgtgac ttggagctag gcgacaggtt cacaacctct ccactaaatg gtgttcaatc
     3121 agctgaccct cagtattagc tcaaaatgct ggttttacaa cctctgatat acggtgttcc
     3181 ataagtagct ttttcggctt cttatttctt gacgccccaa tgtcgacgaa ggaaacctgc
     3241 acgtccagaa ccttctcgat aaagattgta atggagcttg tttttcttgt aataatcctg
     3301 atggtaatct tctgcaggat aaaattcgct tcttggttcg atttttgtca cgattggctt
     3361 atcgaatcgc ccgctgtctt gcaatgcttg ttttgaacgc tctgcaatat ttcgttcttc
     3421 ttcatcatta tagtagatca ctggacgata agaatcaccg cgatcagcga actgtcctaa
     3481 tgcgtcagtc ggatcggttt gctgccaata gatttccacc aactgatcat aagaaataac
     3541 ttctggatca tattcgatct ctactgcttc tgtatgccct gttgtgcctg ttgttacttg
     3601 ttcataagtt ggatttggta catgcccgcc agtataaccg gatgtcacag aaatgatccc
     3661 tggctgctct tcaaatggtt tgaccataca ccaaaagcat ccgcctgcga aaactgcttt
     3721 ttttgtcata taaaatcccc cttttttctc atcatacctt gatttccaaa aggaggataa
     3781 cgttttgctt gtgacagaaa aaacggaaac tattatatac gtgatacggc ttctgataaa
     3841 tgccaaaacg aacgtacttg atcaaaaacg gttaaatcag gaatcacata ttcagcagaa
     3901 ggaagattta tgttttcacc tattcccaat acatgcatgt taccagccaa agctgcttga
     3961 caaccagctt ctgaatcttc aatgaccaag catgcatttg gcggtacatt caattgctgt
     4021 gctcctttga gaaaaacttc tggatcaggt tttgcttttg atacttgcgt gccgtcaata
     4081 aggacatcaa aataacttgt caaattcgtt ttttctaaaa tgaggcgtgc atttttgctt
     4141 gctgatccca atccgatctt gatgtttgtt tttttcaagt actcaagaac agctattgca
     4201 ccaggcaaaa cggatgtttc atccattttt tgaatcgctt gaagatatag attgtttttt
     4261 tgctcagcaa aggctgattt ctctttttct gagaatttct gttccatttt tccaaatgac
     4321 agcaatcgct ctagtgactc ggttctggat attcctttca gttgctcgtt ttctttctca
     4381 gtgaatggaa tggacagctg gttagctaaa accagccacg cttcgtaatg ataacgagct
     4441 gtgtctacaa gaacgccatc taaatcgaat agtgctgctt tgattttcat cgattacctc
     4501 cttgaaaatg aacgacttga agtaaagatg cgatgttttt agctgtgcga tgaagattat
     4561 cagtggcttg ctgtaaagct tcttgtaaag gcacagctgc aggaaggatc ggaaaaacgg
     4621 ccgaaatgcc aacatctgct gctttttcgc ttcctttacc aacacttccg caaatagcta
     4681 tcacaggaac gccctctggt actcttctag caatcccaac tggcgctttt ccttttagac
     4741 tttgtccatc tagacgtcct tcgcctacga ttacgaggtc tgcttgctgt acccgtttat
     4801 cgaactccag taaatccaat acgtagtcga tgccagagac tattttccct gaagcaaatg
     4861 ctaacagtcc tgcacccatt ccaccaccag cactactgcc cgatgcctcg attacttgag
     4921 gggcaaatct catataaaaa tcatgcatgg cttggtcagc cactgctaat tcattttctt
     4981 tcaagccttt ttgtggtccg aaaacaaaag aagcgccttc ttttccgcat aaaagattag
     5041 taacatcagt aatgacagtg attttggctt taatcagctc aataggcacg ttcgttttat
     5101 caactgtagc aattttacct agattttctc ccagtgcttc tactttttga ttatcttgat
     5161 cgtaaaaagc ataacctaga ccttctgcca tgccgatgcc gccatcgttg gtactacttc
     5221 cgccaacgcc gatgatgatg tgtcgaacac cttgttgcaa caattgtcgt atcagttctc
     5281 ctactcctcg cgtggataac ctaagtggct gttgttggtt tagtgggaca tctttcaatc
     5341 cgacgatttc tgccatttca aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt
     5401 tgtttttgcc aaatgcatta gtaacgactg ctgtttgccg cttcaacctt aaactgtctg
     5461 ctaatgatga aaccgttcct tctcctccat cgcctatagg tagcagatca tattcagcct
     5521 gaacaaaaac ttcttgaaaa cctgattgaa tggcttgtgc tgcctctaag gctgtcagac
     5581 tttctttgaa agaatctggc gcaatcagaa tacgcattat tttcccctcc gttccaagat
     5641 cagttctttt tccagctggt attggtctcc attgacaaaa ataggtaatg tttctttcgt
     5701 ctcttttttt attactattt gtttttttgt caaaacaaat agcaacctgt gatttttcca
     5761 aataaaagga aaagaaattt tttcccatgc tgaaggaagt cttggagaaa tagaaagagt
     5821 tccattcgtt actgtaatac ctgaaaaacc aaatataact gctagccata gtgctcctaa
     5881 tgaagcagca tgaattccgt catctgatga atgcggatct ggtcctaaat caatcaggca
     5941 cgcttcttgg aataaatgat acgcccattc catttcttgg atacggcaag cctctatggc
     6001 gtgaatcgct ttactcaacg atgagtcgtg gattgttttg ttttcatagt aatgcaaatt
     6061 cttagaaacg attggttgag aaaaatactc aggtaataag tataacagca tgacaagatc
     6121 tgcttgcttg ctgacttgga gttcatttac ttcgctacgg gcataatcaa gtaaaataga
     6181 ctggctgcca ggacgttctt tgtaagtggt caaatcaata actggcttac tgagatacgt
     6241 gtcatcttgc ggaatcagct gttctttgtt aggcactggc aaatataact tatctaaaaa
     6301 attttgacat tctttcaaaa aaagcggatc gccagcttga tatttaacag ctaaggccac
     6361 attataatgc gccagatagt tcgtatatgc attattgtcg atatattctg tgtattcatc
     6421 tggaccaatg actctgtgta tctctaaatg atcatcgact tttacagctc tgctcagcca
     6481 aaaagctgct gtttcatgaa tgagcgtttt tccctttgtt tctaaaaacg attgatcctc
     6541 tgttgcttgg acatactctt gaacggcata agcaaggtct gcactgatat gatgttctgc
     6601 ggcagctgaa gcaacttctt gtcttgttcc ggttttgata ttcaatgcag caaatttggg
     6661 tgtctcttcc tgaccagtgt gtgcgctttc ccatgggaaa agtgcacctt gatagtgata
     6721 atctcttgct ttttgccgtg cgccttctaa tcgatcataa cggtacagca gcaaattctt
     6781 tgctacttca ggaaactgat ataaaaagaa aggcaggata aaaatttccg tatcccagaa
     6841 aacatgccct ttataacctt cgccagttag tcccttcgcc cctacactaa cacttggatc
     6901 attgcgggga gtcataatct gtaaatggta agcagcgaag tccatcgcta actgatcgaa
     6961 agaagaatcg ccttggatcg ttatccgatg ctgatgccaa aaatctgtcc aataagacat
     7021 cgactgctct aaaatttctg tataactaac gcttttctgg gcttgggcaa tacttttctt
     7081 aaacgggtct gtttgctgaa gagaggtaga gagcacgatg atcttctcca gcgtaaagga
     7141 cgtatttttt ctgatctttt ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa
     7201 tacacctgct ttattgaatt tagctgcaat cccaatatca attttacttt ctgttgtctg
     7261 gtaggatgca ctgatcaaat cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg
     7321 tgtaccaaaa ttggtttgct gtccatcgat acccgttttc acatctactt ggcagtcttc
     7381 ggtcaacggc ttgatgacca ctttcagggc tgcctgatgg agattatttt gatcagcaac
     7441 acgacaagta atgacttcca ctttttttcc ttgagcagtt tcaatgagcg catagcgttt
     7501 aagctctccc atttctaaat ctagtgcccg ctcgtatgca agaactttca tcgtctcaag
     7561 agaaaaatgt tcaccgtcga tttttatttc ccatctgatg acatctggga gattcactaa
     7621 ctcggctgat tctgctccta aaggtcggtt atatacacca gctgcaaaca ttccccttac
     7681 ttgattccga tagtcttcct cctggcttcc tctgatacca aaaaatccat ttccaatggt
     7741 catctgactc gcatacttat tgatatactc taaatcaaac tgtttttctt ctactttcat
     7801 aaggtaataa cttctcctgt ttctgccgaa cgatataaat tttccaccag attctggatg
     7861 acatatcctt gccagccatc tgcaatcatc tgagtctggt caccgagcgc ccgattcaca
     7921 aaatgagcca tacttagttc atgtgggtca ttaactggcg tggtcttttg taaggaaacc
     7981 agctttccat ctacatcttc gaatagttcg cctggataca gattagcacc tgcctgatcg
     8041 ccacaaaaag ccacattcag tttgtcttct ctgatattca aagcaaacga agtattcagt
     8101 tggatgatct tgccgttttc taattccagt gtgccaaaaa ttgcatcttc tactgtgaat
     8161 ttgcttggat cccatttccc gaatttccct gaattttttt tcgtaccaat tttttggaag
     8221 ctgtacgccc gaacgctttt tactttagga aatccagtaa tatacatcgc tgtatcaagc
     8281 atgtgaatgc cataatcgat caaaggcccg cctccttgaa gttctttgtc gataaacgtt
     8341 ccccagcctg gaacaccaga ccgacgaagc gcattcgctt ctacgtaata aatatccccg
     8401 agaacatcaa gatgattctt gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca
     8461 taagccaaaa tctgcccgtt tttatcggct gtctctgcca tctttcttgc ttgatcagct
     8521 gtcatagctg gtggtttctc gcacatcact gaagcattag cttcgagggc agccaataca
     8581 tgttcgaagt gaaagcgatt gggtgtacag acactcacga tatcgggaga aatctcattc
     8641 agcatttgtg taactgatgt atacgcatga gaaaatcctg tacgtcggca aaagtcttct
     8701 gctcgctggc tgactggatc aaccacagcg gccaattcta agacatctga tcttttttga
     8761 taataagcag catgtacttt ttctgctact tgtccagcgc ctatgattgc tactttcttc
     8821 atttatttct ccctcctttg cttatttact tattgttatt ctttctctat tctttctcta
     8881 aatcatttaa atgacgcgct gtttccagac agtttttcaa gtattgcaat gattcctgat
     8941 aaattttcac ttcattttct cctcgcaaac gacattcata taccatataa ccttcatact
     9001 cattttgaag tagcgtttga aaatggcgag taaaatcaat tgcgccagat cctggttggt
     9061 agcgatgatt atctgctaaa tggatatgac ccacatactc tttttgcgta gtcaatgctt
     9121 ttgaaatatc atcttcttca atattcatat gataaaaatc agcgatgatc tgcacatgtt
     9181 tcaattgatt agagtcaatg taatgacgcg catccgctaa caaattgatc atatggtctt
     9241 ggtatcggtt caaaggctcc aaataaataa ccgtacctgt ttcttcggct actttatcta
     9301 aataacgaag tgattcgctg actgctttat agtctccttc tttgcttcgg ggtgaaacca
     9361 tcggcggtaa tcgataagtg aacattcccc aagctgctgg gataacgatt cctttgcctc
     9421 caacctcgct tagtgcacgc aaaatctcag caatttgacg tacaccatta agacgccgtt
     9481 cttcgataaa gtcaccgatc cacccatcgt agccgttaca agctgtcact actgggatcc
     9541 ctgtttcttt gattgcctgt ttgactgtgt cgatattttc tattaacaac ttaccgtcga
     9601 tttcgtattc ttcaaatcca cagtctttga taaatctaaa tttttctaaa atgtctcttg
     9661 ggaaaaatgc ttgatcttgc gtaccgaatt tcattgttct cctcctcgga cttgaacgcc
     9721 cattttgata ctttcttctg ggtgctggtc tacataacgc atgtaagctt ctgcgctttc
     9781 tgcaaatggc acaactggat caatgatttc ttcacaatcc aagtaaccgt tcatcaataa
     9841 ttcccaacat gtgtcctcga ttcgtttacg gttccatctt gggtaatctg ggtttggttc
     9901 actagatgct cgagcaaaga cgatttttgc attattgaaa tgtgcttcgc gaccaagatt
     9961 aaacccagct ggaaaaggtt tagcaaaggc aacataagaa atgatcccgc catagccgat
    10021 ccctcttaaa gaagcttgta atgcggcagc actgccactc gtttcaataa ctgaatctac
    10081 tccacgttta tctgtcaatt cctttagacg aaaaccgaca tctgctttca taggatctag
    10141 cgtgtaatcg gcaccgtatt tttctgctaa ttctctacgc tgcgctatgg gatcgatccc
    10201 aacgatggta gtagcacctg cttttttagc taattgaatc gcaatttgcc cgatagcgcc
    10261 taaaccgaag attgctacag catcacctgc tcgaacatgt gcttcccgga taccacctaa
    10321 agcgaattga gcaggatcgt aacatactgc atttttccat tgatccaacc gatcaagttt
    10381 acgtagttta taattatcaa caccgtttgt aatcagcgtt tcacgaatgg gtccataagt
    10441 gcagactaac tctccttttt gatactctgt caccaaactg cccgtttctt cgatcgtacc
    10501 gacaatcata tttcctaatt gaaattcgcc aaataccaca ccgcgttggc ttccttcttc
    10561 tcttggcaag aataggcgcc attcttctga aaattcttca tcgataaatg gacttaatcc
    10621 tctaaagtcg acaatttctg ttcctggttt tggagtagcg aaaattactc ggatacgtac
    10681 ttcgttcttt tcgatctcac ggtcttcgta atctttgaat ccggctattc ttggttctaa
    10741 tgcaactaat tttttcatct tttctttcct ctttcttttc tttttatcct tttacgccgc
    10801 cttcgactaa atcaccttta ataaatcgtt cagataacgc atacataatg atgactggta
    10861 aagcggtcac caatgaagcg gccatcattc gtccccaaat gtagtctggt gtactgaata
    10921 atgtgttcaa accgattggt aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa
    10981 ataaataatc attccacgaa accataaagc aataaacgaa gacagaaatg attccagaaa
    11041 tagccaatgg tataatgatc cgaataatga tctggatccg gctaagacca tcgatcattg
    11101 ctgcttcttc aatatcagtc ggaatcgtgt caaaataact ttttagcatg aatatcgcag
    11161 taggaagtgt ttgaacgacc atcgtgataa tcagtgctgt attggtatca tacaaaccta
    11221 gaccggaaat gattttaaat aatggaacaa cgatcaaaat accggaaaac atataaacgg
    11281 tgtaaaagct agcattgatt ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg
    11341 ccccgctgat cccaatcact actgcagtta cggaggctat caaggagatc actaaactat
    11401 ttttgaaata catgatgaat ggaaaaatct cagcattgaa aatatcctga taatgttcaa
    11461 aagtccattc tttcggaaaa atagtttgtg tcgttgcaat tgcttcttgt gaatccttga
    11521 atgatgtcat cagcatgatc aaaaatggaa acaaattgac agctaatagc aacaaaatcg
    11581 tcgcgtaaaa taagattttc ctgctctttt tatttttcat ttcaagtcca ccttctttcg
    11641 tgagagcaag acgaccgctg caataatgat gaacaataga atcgatatag ctgctgcttt
    11701 tccaagatca ttgaacgcaa aggctgtctg atacaaataa acgcctaaga tattgacttt
    11761 attcgtcagt agatagacat ctgtaaacat atagaacatc cagatagatc gtaaaacaat
    11821 cacagttgct agtactggtt tgattgccgg cagtgtcacg atcttgaact tctgccaagc
    11881 atttgcaccg tccatttcag ctgcttcata aagcgttttg tcgactgttt gcaaaatagc
    11941 caaaaatgaa ataaacgcat acggatagta tctccagata gcaaacaaaa cgaccataaa
    12001 gaagctagca actggctggt cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc
    12061 tactaacaga tcattgacga ttccataact attattgaac atatatttcc aagcaaaaat
    12121 caaagaaata gatggcgtaa cataacttaa taaaatcagt gaacgcaccg ttttcctaaa
    12181 tctgaacttg cggttaagca aaatagctac ggctaatcct aaagctgtac tgcctgctac
    12241 aactaagaat gtgtaagcaa ctgttaaacc taaagaacga taaaattcac tatcacttaa
    12301 aatttcaaga taattgctta atccgataaa aacggcgtct gagttaggat tcagcggcat
    12361 atcgaaaaaa ctgatctgaa tattggaaag cattggaatc agcactaaaa cagctaataa
    12421 aagcatgctt ggtgctaaaa gtcccatggc taattttaag tcagaatgtt tccttggacg
    12481 attcattgaa acctccccct gataatctgc caatagctat ctactttttt cgaacaccat
    12541 tggcagaact tcttgttatt tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc
    12601 ttcgtttccg acagttactt ggttcactgc ttttccgatc acacctgagc ttgtgatatc
    12661 tcccattttc gtgaaattct tttccccgac tagtccaaag acttgtactt catcaaaagc
    12721 actagcaatc tcagatggca gctcgccaaa agacttgatg acttcatttt ctttgtaacc
    12781 atccagttcg actacttgtt tattcactgg ttgagcacca ccaggagaca tcaagaccca
    12841 cttttccatg tttttcggtt gagataagaa agctgtgaat gcttcagcag cctctttttg
    12901 tttctcgtct agaccagaag aaatcgtcaa tccagatact gtgccataga cagcttgatc
    12961 tttttgtgtt gggatagcaa aaccgatatt ttctgagttc cccgcttcat aaacagaagg
    13021 caaaatgtaa gttgaataga ttgccattgg tgctgttcca ttcatgaaag catctttgat
    13081 ttctgtcaca tcattagagc ctggcatcgt gtattgggat aagttttgat aaaacgacaa
    13141 tgcttctctc atcttttccg tatcgatcgt tacctcgcct tcatcatcca aaacatttgc
    13201 tccatttgac aaagcaaatt gagagaacgc ttgctcactc attgttcctt cagcagtagg
    13261 catagcaatt ccatattgtt tattctcttt atctgtgaaa tgttgagcaa tttttaaaat
    13321 atcatcccaa ttttctggtt ctgaaaaacc agcttctgat aatttttctt tgtcatacca
    13381 aataccttga acccagccgc taattggtgc agcaatatag ctttttccat cttctgaacg
    13441 aacaaggttt ttggctccgt cataataatt atcttctcca gcttcctcga taacgttttg
    13501 gactgccgtc tgatcaatca gttcatcttt atccatcact ttagcaaaat cctgactgac
    13561 ttccattact tctggtaatt taccggaacg agctaacgta acgatttttg tattgaacgc
    13621 tgattcttct accggcactt gcttgatttt gatgtctgga ttttcttttt caaaatcagc
    13681 aaccagttca ttgatcacac ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat
    13741 cgttgtctgt tgtgattcgt tagagctttg actttcacca caagcagtaa gaagaaatcc
    13801 agtagctagc agacaggtag taacaataat ttttttcatt tttttccacc tcttatttta
    13861 ttattttcag ccacatatat tcatacggct ggaatatcaa ctttcgttct tgactgcaat
    13921 ggataatttg tccagaaaac aggtctaggt aatctccttt tggtaaaaga taacctgctg
    13981 attgattgga aaaattatgg ataactaaga ctttctcttc cttcgcttcg cgaataaaac
    14041 tgataaaaga atctgctgag tccagtaagt aaatcaaact attcggatgg aaggcttttt
    14101 ctttttttcg aatagatagc atctgggtca attcaccata tactgctgtt cttaatggat
    14161 ctgtctgcaa ctctttctca atatctgcta acaaaaattt ttgccgattg atttttcgat
    14221 tctctcctgt tttttctacg cctttgtaat catttcgact tcccagcaca ctttgaatat
    14281 aaattgccgg cacgcctggt aacgttacca aaaatgaatg agcagctaaa aagcgtttgc
    14341 ttctccattc cgtcgtatct gttggagaac ttaacgcatc tagatacgta gcgtttatct
    14401 catatggact ggtcgttcca tctgggtttt tcttgtagtt tactcgggta ccctctttct
    14461 tcagctgttc cactaattca aggatcgttt tttccggcag aatacctcgt accggattca
    14521 atccgatacc gtcatgggag gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat
    14581 cgatggtttt taaccattct ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg
    14641 tcaaaggtgg tagcgaaaac tgatagacca tctgtgcttc attctcgcca ttcccaaaat
    14701 aactgatatt atctttgtgt ggcacatttg tctcagtgat caataccgtt cctggagctg
    14761 cttcatcagc gattgcacgg aataattgga tgattttatg cgtttctggc aggtgtatac
    14821 aagaagttcc tagttctttc cataaaaatc ccacagcgtc caagcgaata aatgaagcgc
    14881 ctttttctaa gtaaaacaaa agaatatcga tcattttcaa taaaacttct ggatttttaa
    14941 aattcaagtc tacttgatct tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt
    15001 tgtcaatgaa cgttgtaagt aacgggctgg tccttggtct agtcacgctg cttgtatctg
    15061 tatccagtgg caaatcaata aaaaaatctt tgtacgtatc attcaagttt aaatattcct
    15121 gaaaccaagt actttcggca gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt
    15181 cgcttctcat ttcttctata tcttcccatg tgcccaaacg gtcgttgatt gcctgataat
    15241 ttacaacgga gaaaccatca tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc
    15301 taaatgtatc tgccaaatac ttttgataaa atttcttaaa tgtacgcaaa gttggttgct
    15361 gctcttcaaa aaattgatcc ccataagtaa tcaagaaaat atcttcttcg tcccaatgca
    15421 gtttgcctgc ctggcctttc ttctgctgat aaacactgac tttttgttca atcatagagg
    15481 cggttttctc agacaaatct gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg
    15541 gattcccctt tcttttttgg gagcgttccc aacgatttga tcatattatg gaaaacgctt
    15601 actgtcaata gcattttttt aaataatttt gatatacaca aaaatacaaa taagaaagct
    15661 tatgtatcaa taattaatag cttcttaaaa aaatatggtc tctcatgatc atgcaagcta
    15721 ttcctaaatt ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg gaattattct
    15781 aaaacgctcc caaaaaggag gtgcatataa aattgaaacc tactatttat gacattgcga
    15841 aaaaagctaa tgtctcaaaa tcaactgtct ccagagtatt aaataaccag ccaaatatct
    15901 ctgataaagc aaaagcaagt gtacttcaag caatcaaaga attgaattat gaaccgagca
    15961 aaattgcaag aggactgtca ggaaatggtt ttgacgcgat tctagcatta tctcatcgta
    16021 cttctcatac gacctatggc aatccttttt tttcagatgt tattcaatcc atttctgccg
    16081 tttctgaaca aaataacttc gacttgattc tacaaacggc aaaaacacca gaagaagagc
    16141 tagaaaaatg cgcgaaaaag atccaagaaa aaatgatccg aggaattatt attttaagct
    16201 ctacgatgga tgaaacactt ctttttgaat tggatcaata caatattcct attgtggtaa
    16261 tcggaaaagt cggaaacgac tacacgaaca tctactcagt ggacacggat aatttccagg
    16321 atagttacga tttggtggaa acactgatca agaatggaca ccgtcatatc ggctgtctcc
    16381 atgctccgac agaagtttat gtatccaatg atcgtctcaa tggttaccgt caatgcttat
    16441 ttgatcacca cctagatatc cgtaatgaat ggattataga cggcggcttt cgcttagaag
    16501 atagcatggc agcaatgaat gatttaatga agctacgaga aagaccaact gcgatttttt
    16561 ctaccgatgc attaaaatca ttgagtattt acaaagtggc agaacaatct ggaaagcaga
    16621 tacctgatga tctttcgatc gcaggattca gtgatccctt attgtcctcc ctattttcgc
    16681 cacagttgac tcggatagac attccgacaa atcagttagg aatcaaagcc acagaacttt
    16741 tattccaact aatccaccac caataccctg aagaaaaaag aatcattctt tcaaccgaga
    16801 ttactctcac agattcgatc caacaacaag aaatgtagaa cagaattata aaagaacaga
    16861 aacactgagg ataagtcagt tactctgttc ttttttcaat cactcatccc tcgttacccc
    16921 ctcccggtat tttagcgaat ttttcttatt ttccatctgt tttagatagc tgtggataac
    16981 tataatgttt gtgttaaact attttttcca cagggaagaa aaaactattt ttaaccacgt
    17041 tgtgtataag tttttatagt tcgttttttt aaacacattt acccttattt caatccatgt
    17101 tatttgggga tacttatctc taaaacataa atagaataac agacatgtct actggttttg
    17161 ttttattcga ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct
    17221 tcccatcaat ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc
    17281 tcttggaaga tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct
    17341 ttcccacttt tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac
    17401 aataatttgt cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct
    17461 tctgcagcca tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg
    17521 acttcttttg gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta
    17581 cctataataa cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca
    17641 tccgtcaaga cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct
    17701 aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag
    17761 actagcgtat tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag
    17821 atatctgtat cacctggatc cattacacga actttacgca gcatttgacg aaccattact
    17881 tcgatatgct tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga
    17941 agcaagtagt tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt
    18001 gatccttctg tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt
    18061 gctgtataag gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg
    18121 gtacgtgttg caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact
    18181 gcttgccctt tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg
    18241 atatcgtctc cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt
    18301 tcaccgattg attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca
    18361 gttgcaaggt tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag
    18421 acagaacgga ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg
    18481 gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta
    18541 cgggtgtaac gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg
    18601 atggcttgga tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct
    18661 tgggccacgt caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta
    18721 tcggtcatcc ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct
    18781 tcacggaagt tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa
    18841 ccacgcattc cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac
    18901 atcatgaaga ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct
    18961 tttgcagcat tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca
    19021 cgacggaatt gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt
    19081 ttttcattca gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt
    19141 ttgtacccta agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt
    19201 ttgaagactt ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct
    19261 tgctctttga tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt
    19321 tggacggtca agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc
    19381 tcgttgaaga tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta
    19441 aatggttttt cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat
    19501 ccgttgcgcc atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg
    19561 cgtccttctt cttccattgt caagtagtag ttacctagaa ccatatcttg ggaaggagta
    19621 acaactggtt taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt
    19681 gcttctgctt gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa
    19741 tcggcattat aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa
    19801 actggttcga atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact
    19861 gggtgttcct tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt
    19921 ttacgtttcg catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg
    19981 aatggtttga ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag
    20041 aaaggtccaa caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt
    20101 tgacggaaac gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta
    20161 cctggtccag taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct
    20221 tgcagcatac gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt
    20281 ttcaaacggt tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca
    20341 aaacgtccac cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg
    20401 tccataacca tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa
    20461 cggcggattg cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg
    20521 gctgcttctg catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata
    20581 gcagcttgga attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac
    20641 aattgttttt tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa
    20701 atgatttctt ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc
    20761 cctttaaagt accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca
    20821 cggcgaactt tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa
    20881 cggatacgtt tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc
    20941 tcacagaaca acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt
    21001 tttacttcac cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata
    21061 ctttcgaatt tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc
    21121 gcaagacggc tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct
    21181 tctgcggttt caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct
    21241 ttcttttcta attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg
    21301 atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc
    21361 aagacacgca tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc
    21421 ggtacacctg gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt
    21481 ccgactacgt catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa
    21541 gcttccagtg cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct
    21601 cccaatggtt gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca
    21661 tcgaccatgt gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat
    21721 ggttcacctg tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca
    21781 cgaactgttt cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg
    21841 atacctaact gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata
    21901 cgtgaaggca cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat
    21961 ggcatatctt cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc
    22021 attttatccc cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt
    22081 acgcctggtg acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata
    22141 ccgccgccgc catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca
    22201 aagatcgcat gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact
    22261 ttccctacaa gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg
    22321 tctaaatctt ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt
    22381 cctaattttg tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg
    22441 tcatctttta ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa
    22501 gtcatgaaac caactaaaac gttttggcct agagccattt cgccttgttc catagatggt
    22561 ccgtctgcca gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg
    22621 ttgtagcttg ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca
    22681 ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt
    22741 ttacataaca aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc
    22801 catggtgctt gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag
    22861 gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact
    22921 tgtttaggtg atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt
    22981 tgagcacgag ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt
    23041 gcttgggcta cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta
    23101 acacgacctg tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt
    23161 actttcgcat aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg
    23221 atcggacaca tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg
    23281 tcacgagtca atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca
    23341 agagggtttg tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata
    23401 cttgccacaa ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg
    23461 atcgacattc tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt
    23521 tgtaaaagtt caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca
    23581 ttgccgatac cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga
    23641 cgaaccgttt ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca
    23701 gggtctcttg gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg
    23761 tcttctgatg gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt
    23821 tccatgactt ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga
    23881 tcaactaatg tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg
    23941 tttactttgt aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca
    24001 ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa
    24061 acgtctttca aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg
    24121 actgtattgc gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca
    24181 aatcctaacg cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca
    24241 taagagatgt cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca
    24301 gtagaaccga atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt
    24361 gaacggacta actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt
    24421 tctgtcatta atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg
    24481 cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga
    24541 gcttctgcta ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga
    24601 ttgccattga agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct
    24661 aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca
    24721 ctgattcttg cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc
    24781 aagctcttca cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa
    24841 tatttcaaat attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa
    24901 aaccaaaaat agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt
    24961 atattaaagc cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg
    25021 caacttcata tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc
    25081 tttttcgcaa cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt
    25141 aattgacaga cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc
    25201 tatgcgaacc aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta
    25261 ctttagatag gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta
    25321 tgatggctaa agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct
    25381 gagcagtgaa aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga
    25441 cttttcaatc catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag
    25501 gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta
    25561 ggaggttgcg cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc
    25621 gtattgcaac ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga
    25681 ttagcagaag aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa
    25741 aacaatcaag acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg
    25801 ctacttggaa tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg
    25861 atgactgtca cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa
    25921 cctggcggca atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta
    25981 ctgttaaata tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca
    26041 aactctaaga tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc
    26101 aaagtactga cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa
    26161 gatgtagatg gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc
    26221 ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa
    26281 gacggcggtc tagcaacagt aggaatcgac tacgaaagct tgggtaaaca aacagggatc
    26341 atggcagcaa aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat
    26401 gatttggaac tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc
    26461 atcaaagaag ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct
    26521 tatcatctgc atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt
    26581 tccgtatctt ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga
    26641 tcgcggcagt aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt
    26701 tagggggtgc ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg
    26761 cccttcttgc agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg
    26821 gtgcgccgaa catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg
    26881 gcataacaaa agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat
    26941 gtttacttgt tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata
    27001 atctggcaat gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata
    27061 tgatttctaa tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg
    27121 ctgatttaaa ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag
    27181 aagttttgtt acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg
    27241 tcatttatcg ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga
    27301 aacttgcttc tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat
    27361 tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga
    27421 aaatctccat catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat
    27481 taatctttcc ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa
    27541 atctactttg ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt
    27601 gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt
    27661 attccaagat cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct
    27721 tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac
    27781 atttttcaaa gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga
    27841 aatcggtttg ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca
    27901 gcgtccagaa ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa
    27961 aacggtgatg gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt
    28021 cactcatgac atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg
    28081 aaggatcgtc gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt
    28141 agcgttgttc aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc
    28201 ataatcagaa agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc
    28261 ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc
    28321 cttcttcctg atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt
    28381 acttcaatta ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact
    28441 ggaagtttcc cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg
    28501 ttttgagcac gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga
    28561 aatccagcaa gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc
    28621 tgaatctacg atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt
    28681 atttgttgct gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga
    28741 acctggacga ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt
    28801 gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg
    28861 tcgttctgct tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg
    28921 gattttatct gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg
    28981 gatcgggctt aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc
    29041 catctctgtc tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat
    29101 ctggcaagaa ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga
    29161 aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg
    29221 tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat
    29281 gagtttgtct tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt
    29341 cacaaaagga cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc
    29401 tccttattct atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc
    29461 tataaagcat ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt
    29521 ttacacgatc aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg
    29581 gtaacgataa aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta
    29641 gtgccttaat ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata
    29701 gcagcatcat caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc
    29761 tcgtcaatta cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa
    29821 tcagcagctc ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt
    29881 gcttgttcca gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg
    29941 ctttcccagt cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa
    30001 tctaattctc gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta
    30061 tcttccactg gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac
    30121 cgatcgtatg cccgatccac actctcgcat agcatatcta aataatccat ttattttcct
    30181 cctttttatt tatcctactt actttttcct aattctagga tacctactta tatgttacta
    30241 cacaccctta ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga
    30301 aaaagatagt gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata
    30361 ttctttaaat aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat
    30421 aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta
    30481 gtcctagttg ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca
    30541 tacattccta aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta
    30601 ggaattgcta gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca
    30661 gacggaaata tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat
    30721 gaaggtttat tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct
    30781 tcaggcatac ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat
    30841 gtagcacttc aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca
    30901 gatgattttt ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct
    30961 aaattgaatg tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct
    31021 gcagattttc cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg
    31081 tttttattgg taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt
    31141 aatccaaatg atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta
    31201 cagtcaccaa tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg
    31261 aatatctctt ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa
    31321 ggaacaatcc ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt
    31381 ataggaacaa atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt
    31441 atcagcgaca atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca
    31501 agtgatagct cagattcatc aacttcaagc gacagtacag atactacctc taccacaagt
    31561 gatagcacgg atac
//
LOCUS       DACEDM010000032        31510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEDM010000032 DACEDM010000000
VERSION     DACEDM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.032"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            48..950
                     /locus_tag="IWO50_001312"
     CDS             48..950
                     /locus_tag="IWO50_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0358444.1"
                     /translation="MNQSVAKTAHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKS
                     NCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSD
                     LPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVIT
                     LVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNV
                     NDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRK
                     SLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1272..2051
                     /locus_tag="IWO50_001313"
     CDS             1272..2051
                     /locus_tag="IWO50_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358445.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            2097..3041
                     /locus_tag="IWO50_001314"
     CDS             2097..3041
                     /locus_tag="IWO50_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0358446.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            3057..3836
                     /locus_tag="IWO50_001315"
     CDS             3057..3836
                     /locus_tag="IWO50_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0358447.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            3848..4546
                     /locus_tag="IWO50_001316"
     CDS             3848..4546
                     /locus_tag="IWO50_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0358448.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            4574..5338
                     /locus_tag="IWO50_001317"
     CDS             4574..5338
                     /locus_tag="IWO50_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0358449.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            5383..6072
                     /locus_tag="IWO50_001318"
     CDS             5383..6072
                     /locus_tag="IWO50_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0358450.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            7299..7979
                     /locus_tag="IWO50_001319"
     CDS             7299..7979
                     /locus_tag="IWO50_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0358451.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            8008..9321
                     /locus_tag="IWO50_001320"
     CDS             8008..9321
                     /locus_tag="IWO50_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0358452.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            10127..10894
                     /locus_tag="IWO50_001321"
     CDS             10127..10894
                     /locus_tag="IWO50_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ0358453.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            10921..11922
                     /locus_tag="IWO50_001322"
     CDS             10921..11922
                     /locus_tag="IWO50_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ0358454.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            11945..13231
                     /locus_tag="IWO50_001323"
     CDS             11945..13231
                     /locus_tag="IWO50_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAQ0358455.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            13265..13918
                     /locus_tag="IWO50_001324"
     CDS             13265..13918
                     /locus_tag="IWO50_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0358456.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            13951..15156
                     /locus_tag="IWO50_001325"
     CDS             13951..15156
                     /locus_tag="IWO50_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0358457.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            15286..16611
                     /locus_tag="IWO50_001326"
     CDS             15286..16611
                     /locus_tag="IWO50_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358458.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            16628..17758
                     /locus_tag="IWO50_001327"
     CDS             16628..17758
                     /locus_tag="IWO50_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358459.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            17784..18869
                     /locus_tag="IWO50_001328"
     CDS             17784..18869
                     /locus_tag="IWO50_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAQ0358460.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            18892..19707
                     /locus_tag="IWO50_001329"
     CDS             18892..19707
                     /locus_tag="IWO50_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358461.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            19691..21409
                     /locus_tag="IWO50_001330"
     CDS             19691..21409
                     /locus_tag="IWO50_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAQ0358462.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            21435..22271
                     /locus_tag="IWO50_001331"
     CDS             21435..22271
                     /locus_tag="IWO50_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0358463.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            22276..23709
                     /locus_tag="IWO50_001332"
     CDS             22276..23709
                     /locus_tag="IWO50_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0358464.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKEDMYNIKNIIFLSVLMIVLTIGMTILYFSTILR
                     YSIIIGLVIVAFLKRTQIMKILKNIKN"
     gene            23726..25387
                     /locus_tag="IWO50_001333"
     CDS             23726..25387
                     /locus_tag="IWO50_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0358465.1"
                     /translation="MERRHGEMRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYL
                     YDQYKDVCSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCD
                     EPVIINFADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDD
                     GKKHKLFSGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNW
                     FDIGHADKYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVE
                     YVRPRIFEYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKR
                     YSVSGDNIQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALL
                     SVSVPRELFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELA
                     KLFHSVDGKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDL
                     KKIELIEALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
     gene            25475..25873
                     /locus_tag="IWO50_001334"
     CDS             25475..25873
                     /locus_tag="IWO50_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0358466.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            25898..26614
                     /locus_tag="IWO50_001335"
     CDS             25898..26614
                     /locus_tag="IWO50_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358467.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            27846..28425
                     /locus_tag="IWO50_001336"
                     /pseudo
     CDS             27846..28425
                     /locus_tag="IWO50_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            28485..30137
                     /locus_tag="IWO50_001337"
     CDS             28485..30137
                     /locus_tag="IWO50_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0358468.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            30481..30846
                     /locus_tag="IWO50_001338"
     CDS             30481..30846
                     /locus_tag="IWO50_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAQ0358469.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            30934..31377
                     /locus_tag="IWO50_001339"
     CDS             30934..31377
                     /locus_tag="IWO50_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358470.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
BASE COUNT        10280 a         4607 c         6743 g         9880 t
ORIGIN      
        1 cccatcttac aacggatgaa cttgtaataa tagagtctta tttcaaaatg aatcaatctg
       61 ttgctaaaac tgcccattgc ttgaatcgtt caagacaaac gatccataaa gtatatctgt
      121 tcttcaagca aggaaaatca gccttagaat attatcaaca gtataagaaa aacaaatcaa
      181 actgtggtag acgtccgctt gttttacccg aggaacaatc agaatatatt caaagaaagg
      241 ttgttcaagg atggacaccc gatgtgattg ttggtcgtgc agcgtttcct attagttgtt
      301 ctgctcgtac catttatcgt atgttcaaaa aggggctatt tgattcttct gacttaccga
      361 tgaaaggcaa gcgtaaaccg aatggacatc aagaaagacg tggaaaacaa actttccgcc
      421 gctctattca tgaacgtgaa aaggattata gccaattctc aaatgagttt ggtcaccttg
      481 aaggtgacac tatcgtaggt ctgaaacata aaagtgctgt aattacctta gttgaacgat
      541 tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca agcgattgat attgaaaaaa
      601 aattaaatca atggtttgaa tctgtaccga aaaacctatt caaatccatc acttttgatt
      661 gtggaaagga attttcaaat tggaaacaga tcagtaatgt caatgatatt gccatttatt
      721 tcgctgatcc aggaacgccg tctcaaagag gcctaaacga gaattctaac ggattgttac
      781 gtagagatgg tttattgaaa tctatggatt tcaattcagt agatgaattt tttattcaat
      841 ctgtcgcatc taaacgaaat aatattccta gaaaatcact ggattatcga acacctttgg
      901 aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga caattaagaa
      961 ctattattaa ttcaatgtta atttttttgt tattttattt tttgtgtaac ttttaaaaac
     1021 acctatgaaa attaaggaaa taaaaatgaa atcgattaat tgttatctta ttaatgtttt
     1081 atttactttt aaatcataaa ttgccctagt caagaaaaca gtttgtggta tgattatata
     1141 gggaaaactt gtttacaaat atttctgtga atatattagt ttaattatgt tttttaaaat
     1201 aaaaagctgt catttttatg taagtgaatt atatcttaat ttatctatca tcaatactta
     1261 ggagtgaatc tatggaaaag ccattcgtca agtcattgac tgctgtggcg gtaggtgcag
     1321 gtgctattgc gatctgtttg tttggttacc atatcaataa tcagcggcaa catcagcaac
     1381 ggatcaatta tgcagaatct gccatcacca atcaaaaaga caccttgact tctttatcaa
     1441 aagaagtaga caagctgtat agtacaaaag aaaaaatttt tctaaaccct gaaataacag
     1501 aagaaacaat cacaagtttg tcaaataagc tatcctatgt caaactttca gcagatgatt
     1561 acgacatcaa agaatcagaa ttgcctaaag aagctgttgc aatccaagat gagaagaaag
     1621 caatcgtgac acagttagat agtgcagaat caaaactgaa gatccagaca gctgtcaacg
     1681 atttgtttac taaaaatgta tcaaactggc agcaagctgt agacgatgtc atcatcaaag
     1741 aaaaggtgac aagtgcagat gtcgctcgtg tacgtgaaaa tatgagcttt tttaaagata
     1801 gtccttggaa aacggttgtt atgcaatatt taggattcgc tgatactcag atcgcgcaag
     1861 tcacgcaact agatcagtta ttcgatacga tgttgaagga tggtcaagtg actgccaccg
     1921 ctacttacga tcaatatttg acggcgctca gtcaaataga gcagatccgg aatgaaaaaa
     1981 taagcgcagc atacgcaaca aaagcacaaa ctgttgctca gcaaatgggc tatagcaata
     2041 taagttattg acagttaaaa cgttgattca agcaaggtta gcaaacggag gaaactatgg
     2101 caagacgaag aagaaaatca agagggaaaa aaatatttct gattatttta gcagttctta
     2161 tcgtattagt attagcaatt gtaggagtag gagcaaaatt atatctggat ctctcacatt
     2221 ctattgagaa aacttacgaa tcagtggaac gaacccaagg agaacgaacg aaaccagttg
     2281 atctggacaa acaagaatct ttctctgttc tattgatggg gattgatact ggagatttag
     2341 gtcgggtcga tcaaggaaga tcagatacga tgatggttgc catagtcagt cctgaagaca
     2401 aacagacaac gatcgtcagc attcctcgtg atacgtatgt cgacatcgta ggtcgtgaca
     2461 caaaagacaa gatcaaccac gcttacgcat tcggcggagc tgccatggcg atggataccg
     2521 tacaaaaata tgtagacatc ccaatcgatc actatgtctc tatcaatatg aaagggctaa
     2581 aggaacttgt tgatgcagtt ggtggtatcg aagtcaataa tgacctcact ttctcacaag
     2641 atggctatga ctttacaatc ggtaaaatca cattgaacgg ggatcaggca ttatcttatt
     2701 ctcgtatgcg ttatgaagat ccaaatggtg actatggtag acaagagcgt caaagaaaga
     2761 tcgtagaagg tgtagcaaaa aaagtattga gtttaaacgg tgtcagcaac tatcaatccg
     2821 ttttgaatgc tctagagtcg aatatgaaaa cagacatgag ctttgatgaa atgaaaaaaa
     2881 tcgctttaga ttatcgggag gcatttggta cgatcaagca agatcagctg caaggagaag
     2941 gctttatgca agataatatc tcctatcaaa aagtcagtga tcaagaactt gatcgtgtaa
     3001 aaaaagaatt aaaagatcaa atgaatctag agaataaata ggaaagtgga gaaaaaatgg
     3061 aagagacaat caacttggaa gaaatttttg aaacattacg caaacacttg gtaacaatca
     3121 tcatctcaat gtttgctggc ttggccatct caggaatcgt aacatttttc atcttgacac
     3181 caaaatacag ttcacaagca caattgatcg tgacactgcc acaatcagac acagcaaatg
     3241 tcaatgatgt caatacgaac ctgcaaatga tcaatacgta taaagatatg atcgtcagtg
     3301 atttagtact aaatgaagtc aaagatcgtt tagaaacaga agacaatgtg aaaatgactg
     3361 ctggacaaat caaagatgcg atctccgtca atcagtcaca aaattcacaa atgttctcga
     3421 tccaggcaat aagcacgaat gcagtgactg ctcaacagat cgctaacaca acttcacaag
     3481 ttttccaaga gaacgcaaaa gacgtgatga atgtcgataa aatctcgatt atttcaggtg
     3541 cagttgccaa tacgacacct gtttcgccaa ataataaatt gaatctagtg attggcttag
     3601 tattaggaat gatggtaggc gtaggtctgg cattcttatt cgaattactt gatcggacag
     3661 tgaaagatga aaaatatgtg acggatgcat taggattccc aatcttagga acagttccag
     3721 aaatgtcagc gaaagaattg aacgctggta tccgtaaaaa aggaacagct cctgcacgac
     3781 caaaagctac aaacgataaa acagccccat cacgcagaag ccgctcaaga gtctaggagg
     3841 aagaaaaatg gcacgaacac agaaacaaaa agcagcacaa actcatgcag tcagtttgat
     3901 cacattaaca aacccatctt caccaatcgc cgaacaatat cgtacgatcc ggacgaatat
     3961 ccagtttgcc tccgcagctg gtcagcaaat caaaacgatt gtcgtgactt catcaggtcc
     4021 tggagaagga aaatccacca ctgcagcaaa cattgcagtc gtttttgcta aatccggtca
     4081 acgagttcta ctagtcgatg cagatttacg taaacccgtc gtctataaga cattccagct
     4141 gaacaatgcc aggggattaa gtacagcact tagttcatca ggaagtgtag cagatgtaat
     4201 ccaacgaacg ccagtcgaga acttgtcgat tttgcctagt ggacctaagc cacccaaccc
     4261 atcagaattg ttaagttcgc caagaatgga tcagatccta gacgaagcaa gacagttatt
     4321 tgacgtggtg atattcgata tgccaccagt agtagccgta acagatgcac agatcatgtc
     4381 ttcaaaaacg gatggcactt tattggtcgt acgtgaaaat acatcacgta aagaatcatt
     4441 gaataaagca aaagaactgc tagagatggt acaagcacga gttttaggtg ttgtatacaa
     4501 cggagcagaa catagcaaag atgctggtta ttactacgga aattaaggaa taagaagaga
     4561 ggagacaaga gaaatgatcg atctacattg ccatatccta ccaggaatcg atgacggggc
     4621 agaaaactta gaagcaagta ttgcaatggc tgaaaaagcg attcagcaag gcatcacgca
     4681 tattttatgc acaccacacc acaataatgg aaaatacagc aatgaaaaat cacaagtcat
     4741 ttctctagtc gcttcgttac aagccgaatt agagaaaaga cagttgcctt tgacgttatt
     4801 ggaaggacaa gaagtacgta ttaccggaac gctcatcgaa gacatccacc gagatgaaat
     4861 tttatttaca gatttagatg atacatatct tctgatcgaa tttccgacac tggaagtgcc
     4921 gttatatgca gaatggcttt tttttgagtt gttagaacta ggaaagacac cagtgatcgt
     4981 ccatccagaa cgcaacgcgc attttcgaga agacccaaat catttgcttc cgttcctaga
     5041 tatgggatgt ttggcccaaa ttactgcgcc aagttatgtt ggtgtatttg gcaaagacat
     5101 tcagaaaaca gccaaaacaa tggtcaaaca taatctcgtg cagatggctg cttctgatgc
     5161 ccatggggtc tctaagcgta ccttttactt aaaagagtgc tatgagcaga ttgctaagga
     5221 ctttggtaag gaaaaagtag tacagatgaa acaagtcgca aaggatctga tcaacgggga
     5281 tgaaatcagt tatccagtct atgaagaggt aaaaaagaag aagtttggtt tattttagtt
     5341 ctttattcgt agataaaaag gaaatgagga gagagggaaa gtatggaaag tgaagattta
     5401 ttatctcgta ttagtgaagt acatcctaat caagtttggt tggataaaga aaaagttgaa
     5461 aaacgatata tctatttatt ttttaaaaga atgattgata ttgtaggaag cattattggt
     5521 ttgattttag cttctccttt gatgttctat gtagcttatc gaatcagaaa agaagagcca
     5581 ggaagtccga tatttttttg ccatacacgt gttggtaaaa atggaaaaac attcaaaatg
     5641 tataaattcc gttcgatgtg tttagatgct gaagaaaaat tggaagaatt acttgatcaa
     5701 aatgaaatag aaggtgcaat gttcaagatg aaagaagatc cccgtgtcac taaaataggt
     5761 aaaattatcc gtgccaaaag cattgatgaa ttgccacagc tatggaatgt tttaaaaggc
     5821 gatatgagtc taattggtcc acggccacct ttggagaggg aagtagctga gtacacgaga
     5881 tatgatcttc aacgcttgta tgtaaaacct ggttgttcag gtttgtggca agttagtgat
     5941 agaaataatg ttagtttcca taaaatggtt gagttggatt tagagtatat tagtcatcga
     6001 agtttaatgt acgacttgag gttgatttta aaaacgtttt atgttattgt caagcccaat
     6061 ggagcatatt agttagggat agaatgatgg attgttaata aaaaagatta cacatgttca
     6121 tattatcgtt tcaaaagtaa taactgtaat tatggagggt ttgaatcact tgtagaaaat
     6181 ctaaaaaaag aaaggtgaat ggggcaattc agtatcatta ttcttatata aaagaaaata
     6241 ttcgaaattt tgggatgata ccactcttaa atataatgat ctaaatgctt taatattggt
     6301 atatccgata taggaactga cgagctattt agtatgatac aaaagtatat tgatagccat
     6361 taataaattt tccaaaaaat aatgacagca caccaatttt tataatttag cttgttgaat
     6421 cggtatgttc atgggacggt tcaaaaaaag aatcgaaaaa ataaacggta aaattttagt
     6481 gaatcctatg taataaaatt tggatagatg gaaaaacagc acataaggag gctgagtttg
     6541 ttgtcatttc tgctcctaca aactatggta gtgaatgtgt tattgaatta gtgatgtctg
     6601 ttacttcaga gacattgggg ataattaact tcactatacc tgtagggtat acaaaaaatg
     6661 gaagaaaaaa tattatcaat agattttatt tagttcagaa tttttacgtg gaagtaaatt
     6721 attgatgata atctttatcc aattagaatt attatcgtag gatgtgatga tgatagccgt
     6781 gaagttgttc aagtatttac caatgtctac aacgcagaaa attagtttgg gtttttcgga
     6841 ttggaaaatt tcaatgtata aaatcataaa tttgctgtaa atagacagtt tggggtagta
     6901 atagcccaat aaattgttcg ataaactcat ctttggggtg agaatagctc aatcattgaa
     6961 tttcaaacct gcctttgggg tgggattaga ggtcaatatg tacctacata atactttttg
     7021 gaaaatgatg gtgttgagaa tgtaagtaca tttcaatgtg aaattctaat atagccccaa
     7081 acgaggtcaa taaaaatgac ctaaactcgt atttctcccc taaacgagga ggagatagat
     7141 atgaaaattg aatgtgccgg cgttggagtt ggctgctttt agtaggaatg aacatggttc
     7201 gatgagaact gtttcagata gagtggtcga tgatcacagg cctttaagtc gtttacagat
     7261 gatgagactt gtaaaaatag agggacgaat ggaaaaagat gattggtata gaagaaaaga
     7321 aaggtgttca gaaagacgta acaattgata gggaagagaa tcttagggga catagattct
     7381 attggattgg acgcagagca caagatatct tcttctcggc tctagctttg attgcattga
     7441 gccctgtgat gctaattaca gcgctcgcca ttgtgattga cgatcccggt ggcagcccga
     7501 tttttgtgca ggatagagtt ggaagaaatg gaaagctgtt caagttttat aaattccgat
     7561 ctatgtgtgt agatgctgaa gcaaaattag aagccttgtt ggcacaaaac gagatggatg
     7621 gtccggtctt caagattaaa gatgacccaa gaatcacgag agttggtaaa tttattcgaa
     7681 agacaagtat cgatgagtta cctcagctct ggaacattct taaaggtgat atgagtatta
     7741 ttggacccag acctgcttta ccgagagagg tggaacaata cggtgactac gaaaggcagc
     7801 ggttgttcgt acagcctggt cttagttgct attggcagat tgctccacat cgtaatgaac
     7861 tgtcctttga agaatgggtg gaacttgatg tgaagtacat caatgaaaga agctttatca
     7921 cggactggaa gattatcttc ttgactttac aggctgtatt atttggatat ggtgaatgat
     7981 tatatatagt attaggagtt gaaatatgtg aatattattt tattatcggg tggtagcggt
     8041 aggcggttat ggccgctgtc caacgatatt cgtagcaaac aattcatcaa actgtttaag
     8101 gatgaaaatg gcgaatatga atctatgatt caaagagttt atcgccagat tacaactgtt
     8161 gacccagatg caaaggtcac tattgcaaca agtaagtctc aggctagtgc aatccataat
     8221 cagttgggag ataaggcttc gatttgtgta gaaccttgtc gtagagatac tttccctgca
     8281 attttgttag ctgcagcata tcttcacgat gaacttggcg taggtgatga tgaaagtgtt
     8341 gcggtttgtc ctgttgatcc ttatgtagat aattcctatt acgaaggcgt tgaagccctt
     8401 gagaagttgg tgcagcagaa tacggctaat ttgactttga tgggtattgc acctacatat
     8461 cctagtgaaa agtacggtta tatcatccct gaaagttcta acgaagtaag tactgttaag
     8521 gagtttaagg aaaaacctga caaagcaacg gcagagaaat atttagctca gaatgctgtt
     8581 tggaatgccg gtgtatttgc gttcaaactt ggttacctgt tggaaaaagc tcacgcaatg
     8641 gtggattttg aagattatcg tgatttgtat tccaagtatg agactttaac caagatcagc
     8701 tttgactatg ctgtggtcga aaaagaaacg agcattcaag tgatgcgtta taacggggat
     8761 tggaaagatg tcggaacttg gaacatgatg gctgaagtta tggctgataa tattaagggg
     8821 aaagctgtaa tggatgatac ctgtgttaat actcagattg tgaatgagtt gaatattcct
     8881 attcttgcta tgggctgcaa agatatggta attgcagcaa gtggcgatgg aattctgatt
     8941 tctgataaag agcgcagtgg ctatatgaag ccgtatgttg agaaaatcag tacggaagta
     9001 atgtttgcag aaaaatcctg gggtacatat accgttatgg acgttcagga aggctccatg
     9061 actgttaaag tttctatgca ggccggacag aagttgagct accactacca caattctcgc
     9121 gatgaagttt ggactgttgt agcaggacag ggtaaagcga ttgtagatgg tatgaagcag
     9181 attcttcgtg cgggtgatgt ggtgactatt gccgcaggat gtaaacatac aatcgaagcc
     9241 gtgacaaatc tggatattat cgaagttcag attggtgaaa ctattagcgt cgctgacaag
     9301 tataagagtt ctttgaacta aaagttcgcg aggtagttat tatggagata ctaaaatttc
     9361 aacccgtggg taaagattat ctttggggtg gagaacggtt taagacggaa tttggaaaaa
     9421 attgacttaa caccattaag cttatacgtg ggaatgctta gttcatctag acgataacta
     9481 cgtacggaaa tatcaaatat gaaggacaga atggtaaaac cgagttgtgg tatgtattgg
     9541 aagctacacc ggggggtgat ttatgctttt gagtacgatg taacagaaga aatgttacga
     9601 aatgctgtga tggacagatc acttgggaaa catttatata aagaagaagt acacaaggga
     9661 gatctttttt atgtcagttc atggtattgg cgctggagta ttagtggctg aaatacagga
     9721 aagctcaaat gttacctatc gagtttacga ttatgatcga gtagataaga atgataataa
     9781 gtgagaattg tactttgaaa aagcgattca ggtaatgaat atggaagcca ctttgaaatt
     9841 ggagaaaaga aataagctca ttcattttta tcctggtggc tctcgtgaaa tactgtgtag
     9901 atgtaaatac tgtgagcccg aaagaatcca agtaaagacg gggttttcgt tctcggtaaa
     9961 agaggaatct tttcaagttc ttttatgtat atcgggggac ggacaagttg agtcggcaaa
    10021 tggtgttatg aaactcatta gatttgtaaa aaagatattg tatgtttttg ccaacgaata
    10081 ttggaagatg ttaagtgttg agcgatgcgg acttactgaa aattagatgg ggatagaggg
    10141 aggatatcta atgtcacgaa tgagatttat gaacacagaa attgacaatc ttacaatggg
    10201 tgaagtattg gtagaggttg aaaaaatgat tcaagatagg agatgcggat atgttgtaac
    10261 tccgaatgtg gatcacattg ttcagttgga aacaggaaaa gagttgcaag atgtttataa
    10321 aaacgcatct ttaattttga cggatggtaa accattgatt tggatttcaa agttttataa
    10381 gacaccaata aaagaaaaaa tatcgggatc cgatatattt tctttactgt gcaagatggc
    10441 tgcagaaaag ggatacacaa tgtatttttt aggtgcggca gagggcgttg cggctaaagc
    10501 ggcagataac ctgatgaaaa gatttccggg attaaatgtt gtaggaacat actctcctcc
    10561 ttatgggttc gaaaaagacg aagatgaaat gtttaaaatt gattcccaaa ttaaacgagc
    10621 aaatccagat atgcttattg tgggtttggg ttgccctaaa caggaaaaat ttatgtttta
    10681 taactgtgat agattgggag tacctgtgtc ttttggtcta ggagctagtt tggactttga
    10741 ggcagggaat gttaaaagag caccaaaatg gatgtcagat tatggatttg agtggctgtt
    10801 tagaattact caggacccta aaagaatggc aaaacgatat ttggttgatg atttaaaaat
    10861 ctttaaatta gcactaaaat atcggaaaaa ataaactata ttcaatgagg acaatgaagt
    10921 atgattatta caaaaacacc ttttcgcatg tctttttttg gcggcggaac ggatatggaa
    10981 gattattttc gagaaaatgg cggtgcagtg ctttccacta cttttgataa gtactgttat
    11041 gtgaatgtgc gtcatttgcc tagatttttt gattacagca cagagttatc atattctcgt
    11101 actgagagag taacaaatat tgaagatatc gagcatcctg caattcgtaa tgcgatgaaa
    11161 atgctagata tgcatgaaat tcgccttaca tatgaagcag atcttcctgc acgttctggt
    11221 ctgggtacat ctagttcttt tgccgtaggt atgttgaatg ctttctatgc tttgaagggc
    11281 aagtacgctg acaagaagaa actcgttgat aaagcaattt atcttgaaag agtcctttgt
    11341 aatgaagcag gcggatggca agatcagatt gcagcagctt atggtggctt taaccgaatc
    11401 aatttcaact cagatggcta tgaagtattg cctattatta ttacgcctga gcgtaaaaag
    11461 cagcttaaca acaacttgat gatgttcttt actggattca ccagattttc ctctgatgtt
    11521 cagaaggcaa ataatgtcag cggcacagag gacaaaagag taagactgaa aaaaatgtat
    11581 gagcttgtgg atgaagcaga agcggttctg actgataaga acagagatct agatgatttc
    11641 ggcagactgc ttgatctcac ttggagattg aaaaaggcta ccggtggtgc gattagcact
    11701 ggaagtatcg atgaacttta tgaaaagggc atggctgctg gtgcattggg cggtaaactt
    11761 ctcggagcag gtggcggcgg tttcttggtt ttttatgttc agcctgaaaa acaaaatgct
    11821 gtgcgttcgg caatgagtaa tttgatgtat attccgtttg aatttgaaaa cggtggaaca
    11881 caggttattc attacacacc ggaaatgtat attccaaaat aaagaaatca gcgaggataa
    11941 gaatatgaaa gttgttatta tggcaggcgg taagggaact cgcatctcat cggttgcaag
    12001 cgatattcca aagcccatga ttaaaattga aaacaaaccg gtattggagc acgaaatcga
    12061 atgtctgaga gaccagggat tcacagatat tattcttacc gttagccatc ttggcgatat
    12121 cattatgaac tattttggcg atggttctgg aatttcacca gttactggtc agccctttga
    12181 cgttaaaatt gaatactttt tggagaaaga gccattagga aatgccggtg cattgtttcg
    12241 tttaaaggat aatttgaccg aagacttcct cctgctgaat gcagatgcga tgtttgacgt
    12301 tgatttcaac cgatttgtag catatcaccg tgaaaagggt ggcttggtga cattgtttac
    12361 tcaccctaac agtcatccat atgacagcgg tctaatcatt gcagataagg atggctctgt
    12421 agacagatgg cttgtaaagg aagattcccg tccaacttat taccgtaaca gagtaaatgc
    12481 gggactccat gtgattagtc ctgctgttct tgatgtcgag ataaacacac ctaagattga
    12541 cttggaccgt cagttattaa agccgttggc aggaaccgga aaaatgttct gctatgatag
    12601 tccagaatac gtaaaagaca tgggtacccc tgatcgttat tatgctgtct gtgaagattt
    12661 taaggctgga agagtgaagg gcaaaaatct tcagaataag cagaaagctg ttttccttga
    12721 tcgtgatgga acgattaatc aatatgttgg ctttctgcgt catattgatg agttcgaact
    12781 aatcaagggt actgaagatg cagtgaaaaa gatcaatgcc gctggatatc tggctattgt
    12841 aataacgaat cagccagtaa ttgcaagagg tgaagtttct tttgaagaat tggaagagat
    12901 ccataacaaa atggagacac ttcttggata taagggcgct tatttggatg cagtttatta
    12961 ttgcccgcat catccacata agggatatga ggaagagcgc ccagagctaa aattcgattg
    13021 tgaatgcaga aagccaaagc ccggaatgtt aattcaggca gctacggact tcaacataga
    13081 tttgagtcag tcttggatga ttggcgatgg cgagaatgat attaaagcgg gccaagccgc
    13141 cggctgtaag actgcattgg ttggcgagag tgagtatgga caggatatga ccatagcaac
    13201 cctgagtgaa tttgcagata agatctgcta agggatgttt tatcacgaaa ggagagtata
    13261 aacaatgata gacaatagat tacagaaaca cattgatttg ctgattaaac gctatcccac
    13321 tcttgaagta atcaagcagg atattgttga cggatacctg attatggagg aatgctattt
    13381 aaatgatggc aagctgctga tagcaggaaa tggcggatct gctgctgatt ccgagcatat
    13441 tgcgggtgag ctgatgaaaa gctttaagac gcctcgccct gttagtaggg aatttgcaga
    13501 taaattgatt gcggttgatc ctgtaaaggg ttctgaatta gctaagaatc ttgaatatgg
    13561 tttgatggcg attcctctcg ttgctcatga agcgctttcc actgcgtata ttaatgatgt
    13621 tgatggccta ggtgttttcg cacagcagtt gtttggattt ggaagacctg gcgatgtatt
    13681 ccttggtatt tcgacatccg gcaatagcaa aaatgtgata aatgcaactg ttgttgctag
    13741 agctcttggt atgaaagtaa ttggcttaac tggagccaaa ggtggagaac ttgcaagggt
    13801 agcagatgtt tgtgttaaag ctcctgaaac agaaacatat atgattcagg agtaccattt
    13861 gccaatttac cactgttggt gtctaatgtt ggaagataaa ttttttggga atagataaaa
    13921 tatctaagcc catattgaaa ggataaaatt atgaatgtac tatttataac aactcaattt
    13981 ccatatcctc tagataatgg cggaaaaatt ggtgcgtaca acggtatcag tgttttatct
    14041 agagactacg atgtgacggt attgtctttc accgaagaac cgcagtatgt tgaagaaggt
    14101 gtcagttttt ttcgagagaa atttaatagt gtaaaatttg aaaatcctgt gatacatgat
    14161 gtacacataa gaaataatcc aatgaaattg gtagaagctt tagcaatagg gtattgtcta
    14221 cgccaacctt atatagtttc aaagttttat gattcaaaaa tgctgcagtt aatagatgaa
    14281 aaattttcta acaatgcata ttgggatatc atttttattg actatctgaa tatgcaaagt
    14341 tatggtcaat acatacggga gaagtataaa acacaatttg gtaagtatat cttaaaagac
    14401 cacaacatag aatatgattt agtcctacag gaagctgaat caagtcgagg tttaaaaaag
    14461 aagttgcttc agttagagta taagcgtact cgaaagtatg aagaggccgc agtaaatagg
    14521 gccgattatt gctttagtgt gtgtgatgca aatacggaag tactgaaaaa gattaatgac
    14581 aacagttata cgatgcttcc aacttatgaa atgcttacag gggtgaaaaa aaatatagag
    14641 aagaacacga tcctatattt gggtaacctt tcttggaaat ccaatatgca aggattagtt
    14701 tggtttttag agtcaattta tccaaatatt caacaaagaa ttccggatgt aaaaatcaca
    14761 attgtcggta gcgggccagt tggaacagat tttagtaaat attcaggcgt ggattatcgt
    14821 gggtatgtaa aagatatttc acacatttat gacgatcaag cggtgctaat tgtacctttg
    14881 ttagaaggta gtggtataag aataaaaata ttagaagcgt tcaataatga aattgcagtt
    14941 gtatctacat tagttgggtg tgatacgatt ggcgcaaaag atggaagtga gttatttata
    15001 gcagataacg aaaaagattt tgcaacggct gtagttagac tgcttgaaga catgaatttg
    15061 aatgaaaaag taagaaagaa tgcaaaatta ttcttaaaag aaaaatactc cttaacggct
    15121 agacaagatg agtttagtgc gataattaac agctaatata tatataagct tatctgatga
    15181 tgatttatag gaggaaatat atggagatgg ctgagagaaa ggaaaagaaa cttggaaata
    15241 agaatatcat attattttag aaaactcctg ttattttatg tttgtgtgat tgttgcggct
    15301 atgagttacc accttacagg caattccaat gtagcgtcat taattgctgt agtaggagta
    15361 gtttatgcta ttgtaaaggc gtgtaataac gagtatctgg ctatatgggt tatgtttgta
    15421 ttaggttctg atatatgggg gatagtgcca tatattaaca ttggtagtgg tgtgttgcat
    15481 tggtcagatt ttaatgttat agtagcgata tttctctgct tgagattcat caaagttaaa
    15541 gctaggagta gatacgggat tgtctatagt gtaatgctta tatttatttt gattgcatgt
    15601 gtgcaatcgc attcaatcta tggacaagat gtacatacaa cgctcttaac agctagagaa
    15661 ttgctagttg taatatcctt ttggccgata gtaaaaatag tatattcaga aagaataact
    15721 aaaaatgaat tcattgattt agtgtgtaaa ttcacaagat ggacaatgtg tatatattgg
    15781 tttgaatgga ttcttgttaa tatgggattc aatataacct ttttacagac aggtgcacgc
    15841 tggggtacta gaatctatat agatccgata tttatcattt tatatttctt tataaatcta
    15901 tatgcggtta tatataaaag agagggctat aatagtacaa ataaaagtat atggaattgc
    15961 atttttagtt tactgacatt tgttataatc tcgcaaggca gaagtggtat attatatgta
    16021 gtaataacct cggttattat gcttttaatt tcgcttaaac catccaagat tattagattt
    16081 ggtatattag gaattgcctt aattgcatgt ctagtgtctg tgacccaaat tagagaggtc
    16141 gttatatctt cgtttgtaga gtctcagagc agtgacacag gtaatatcgc atatagaatt
    16201 gctgaacaag attattttga aaataaatta gaaggtcatg aactatttgg agttggtata
    16261 ccaaacaatc atgatgccag agctgtagaa tattcaggca aaacaataga ttttgagtct
    16321 aaaaaatata atccctatta tcttgaggac ttaggcgctt ttgaaattcg ttatagattt
    16381 ggtataattg catatgcgtt atatatagtt gctttagcta taagtattat agatgccgga
    16441 tttcgaagag acaaatcggt cgccgcattt gcgggtatgg cattcatggt gtttatggcg
    16501 cttaatttta atttgctagc gtatattaca gcaagaccgt tcggatttat gatttcttta
    16561 attttgatag aaatatcggc attcaatgat gaattcatat atattgaata ggtaagggag
    16621 agcacagatg aatttagatc gattaaagaa aatagcaaat atgatttcac caatcataaa
    16681 agtgataaat gcgccgttag cgtattttag tgggaaatca gattatatgg agaagggaat
    16741 attgtggcct actgtaacaa cctactccaa taacagtaga aaagtgattc aatatacaac
    16801 tttacctaaa aacgtagaca acatggctca aatgcaatgg gctggggaaa gttttgagga
    16861 catatgtata gttcttcaag gtccagtatt agaagacggt gattttacac taaataccgt
    16921 taaaatgtat aataaatact atcctaaagt aaaacttgta gtgtctactt ggataggatt
    16981 atctgaagag acagaatgca agttgcgtga agctggtgca gatgttgtat tatgcaagaa
    17041 acctgaaaaa acaggtgcgt acaatattaa tctacaactt acttctgcat acgaaggcgt
    17101 aaaaatggcg caaaaactgg ggtgtagtta tgcgctgaaa acgcgaactg atcaaagact
    17161 atatgcaaat gatgtactaa agtatttcca aacattattg aatatttttc catcttcgga
    17221 ctgtaaagta gtgaatcagc gattaatatt tacgacttat ggaaaatcat ttagatatct
    17281 tccgtttaat ttatgtgatt ttttggtata cggagaaact agtgaattat taaaattata
    17341 tggtattcaa agagataacc gagcatcaga ctttgggaaa attatttata ctgaggaaaa
    17401 agagtttaat aaaaagatat ttacactact agaaggaaaa caaggtgtat ctccatatga
    17461 tgtttttcca gattttgatg cactatataa aaaatatatg tttgcagaat actatattgt
    17521 gtataattat ttcctggaaa atattgcaag taatgaatct tatggtgact taatggatgc
    17581 ctattatagt tatttaaaga aatatgcagt tgttgctgat tcagaaaaat tgttactata
    17641 ttggccgaaa tatgttcaat ataatgttaa gttcgaaaat gaacttgatt tctatgcgaa
    17701 gttggattca aaaaaatgga tggatattta tgctaattat acacctttta gtcagtgatt
    17761 atatggaaag gatgaggtga ataatgaaaa tcggaatagt gactattgtt aatggggcaa
    17821 gttttggtaa cagattacaa aattatgcga ctcaaaaagt gttggaaaat aacggctata
    17881 cagttgagac aattaataat tgttttgaca aagaaagtac cctatccatt gttatcaaaa
    17941 acgctattta tacattattt aagtttatac cgcatttcaa aaagtcaaaa agtatctgga
    18001 aaagaatgta tacattttat caatgggaca agcagtatat agattattct catcgtaaaa
    18061 ttgatgcatc taaaaaaaac agctttgtga gtataaagaa tgattatgac tatttcttgg
    18121 ttggtagcga tcaaatttgg aatccaaaat atgcagccaa taagggattt gaacttttat
    18181 catttgttcc aaatgaaaaa agagtttcat tatcaaccag tttgggagtt ccatctatac
    18241 caagtgattt agaaaaatta tatgtgacag agtggaataa gtttaaatca atatcggtac
    18301 gtgaaaatca agcggcaaaa ataattgagg acttgacagg cacaactgcg gctgttcaca
    18361 ttgacccaac tttaatgttg gagcgttcgg aatgggaagc ctttcaaaaa aaaccagaca
    18421 atattgatga aaattacatc ttgtcgtttt tcttaactaa tccgcctaag gatactgttg
    18481 atgtattaga taaggtcgct aaagatttga aaataggtgt tctcacctta gatgcgtctt
    18541 gggaatattt tcaagatatt actccgaatc attttgttta tttgatttca catgcaagaa
    18601 tgatttatac agattcattc cactgcacag ttttttcatt gctatttgga aaaccggttg
    18661 tggtatttga ccgttttgat agtgaaaaaa tgaattctag gttaagtaca ttgttaaata
    18721 aagtaaataa agcacatttt tgggtaggaa aaaaagctga tgaagcaatg attcgtgcaa
    18781 tagcatctga acctgaatat gacatagata agctgatggt atcagagagg gaatgtgtaa
    18841 aagcttatct taaagagtat ttacagtaaa attttgaaat gggatgaaaa tatggagcat
    18901 ttaagtgatt gttttggtaa aatgaagatt gttacgactt catgcgcaag agaatatttt
    18961 aaggatgatt tggataaatc agagtattca tgttttgttg attctcgagc agctatgttt
    19021 ttcgcaatgg gacaagcttt agctaaaaga aggaatgtag ttgtaatgct tgctgatgaa
    19081 ttgacatcgt gttatacggc actaacagaa tgtgcatttc aacaagttga tttaattgtg
    19141 tttgcttttc agacagtcag acatgaaatt gatttagcat ctttgaagaa ttgtggttgc
    19201 aatctaatta actgtagcgc ccaagacgat attgatagag ttgtgtccaa cactaagggg
    19261 ttaaatcaga caacagtaat taaaatcaat atgaattgtg agtcggataa agatcatata
    19321 tgtgagtata tagacctgcc agacatacct gtttttttcc acggtagctt atactcacct
    19381 gcaagtaaaa agaaatatga gcagtatgga caggtatcga agtatttagg ctatctcgtg
    19441 gggggaaaag atgcaatact cataatttct cacaatgagt ttttaaacga tatcagtgta
    19501 tggattaatt attattcttt tggagaaatt caacccaaaa ttattatatt tgacagtccg
    19561 aatgactatc aatataaatg gctgtgtgac ttagaatgca gtgtagctca ttcaaccaac
    19621 cgatttttgt ctgaggattt agccttattg caaaataacg gaatttccat tgtgttaaga
    19681 ggtgataatt atgatatcgt cagttaaaaa tgttcaaatt ttagtatctt tattaaaaca
    19741 gcataatatt aaagatatag ttatctcgcc aggtagtaga gattttcaat tggtgcatag
    19801 tgtagaaaca gacagttttt ttagaaccta tacggttgta gatgagcgca gtgctgcctt
    19861 ttttgctaat ggactttcta tggcgattga taagcccgtg tgtgtatgtt gtacttcttc
    19921 aactgcctct tcaaattata tggcggcagc taaagatgct tttgctaatg gcatccaact
    19981 tgtacttctt actgctgata gagattaccg ccgcttatat cagatggaag atcagatgat
    20041 tgatcaggta gatatgtatg gggcttatgt gcgttctaac gttgatattc ctattgttaa
    20101 ggattctgat gatgaatggt atgctaccag aaaaataaat gaagcaatat tggagttaaa
    20161 tcatggcgtt aagggtcccg tgcagattaa ttatcaatta atggatttaa gtgcgccgtt
    20221 tgttgagacc atgccagagt atcgtaaaat tacaagatat caagaggatg atttactgtc
    20281 atcagtagag atttttaaac agaaactatt atcaaaaaag agaataatgg ttttatgtgg
    20341 acagcaggaa gaaaataatt taagtgcctt attggaagag ttctttatta aatataatac
    20401 aattataatc aaggataact attctaatat acacggtgaa cactttatta agactgttct
    20461 agtaacagaa agaatgagtg taaatgcatt tattgattat tgcccagatt tggtaataac
    20521 tattggtaaa catgtatggt cttttgtgaa atatactctt agagataaaa agcttaaatt
    20581 tgagcattgg cggatatctg aggatggtaa gattgttgat ccgttcaaca agctaacatc
    20641 tgtctttcag tgctcagcgg aattttttat ggagactgtc actgctggtg tgaatagcaa
    20701 taataatctt gagtattata atctatggaa gacaagatta tcaaaagttt gctatccaga
    20761 gttagtctat agcaattttt atgcaatttc agaactagta aaaaaaattc cagatagtgc
    20821 attagttcat tctagtattt taaatgcggc tagattgtat gactttgtat ctgatgataa
    20881 agcattactc agttttgcta atttaggggc tgatggtatt gatggttgtt taccaacatt
    20941 tttaggtcag gcagaagcag ataaaactcg acctgcgtat ttaatttcag gagacttgag
    21001 tttcttttat gctatgaata gtttatgtgg tgacattcca gataatgcac atattttcct
    21061 tattaacaat cattctggaa gtgaattcca tacaaatttt ggtaagtttt acaacggatc
    21121 attaagtgtt gataagcata tagctgcagg acatatttca aacgcaaagg attgggcatt
    21181 agaaaataat ataaaataca ttactgcgga ttctaaggaa agtctcaaat cggggttgga
    21241 tgtatttatt aatacatttg acagtcctgt tttatttgag gtctttacag atccggatac
    21301 ggatacgtct gttctggaat ctttttatag tataaataag gtaatcacac caaaaatgta
    21361 ttttagaaag gcgttagata ttataaaaaa gcatttacac attaagtaga tgtttattgc
    21421 gaggtgaaac aaatatggca ataaaatctt atattaaacg gttattgctt tatatccgtc
    21481 atggagatcc caaaataaat ttagatgtga gttatttatc tgaaggtgca atgcttgctg
    21541 gtaagactgt gataattacg ggtggtgcaa aaggacttgg ttttgcaatt gcaaaatcat
    21601 ttatagctca aggcgcaagc gttattatta caggaagaga catggattcg ttgaataatg
    21661 ctaaatgtca gcttggagat atgtgttcta tcagggcgtt tgatataact gatgctaaaa
    21721 tgtatcgaac gttttttgaa gatgcatacg agacctttgg acgtattgat atactgctta
    21781 ataatgctgg gatctcgtta cacgaagatg gatttatgag tgttactgag aaaacttggg
    21841 ataagcaaat ggatacaaat ttgaaaggtg tttatttctt tacacaggaa tttattaagt
    21901 ttgttaaaga aaagaatcaa atgcaagcaa atattattat gatgtcctca gaaagaggct
    21961 tgtgtggtga tagtattccg tatggattat caaaggctgc aattaactct ttagttgtag
    22021 gcttgtctaa gaaatatatt tctgagggaa ttagggttaa tggtattgct cctggagtaa
    22081 ctgcaaccgc tatgactggt atttctgaaa agggtaattt gtataatcct gcaacgatcg
    22141 gttatagata ttttctccca gaagaagttg ctcaagttgc tttgtttttg gcaagtagtc
    22201 tttcaaactg tatcagtggt gaaatcatag cctgtgacca ggggcaatat ttaagtcgaa
    22261 attgggggta ataacttggg aatattatta aaaaaatata atagccttag tgagcctgca
    22321 aaagcatcta tttggtttac actttgtaat gtgttgcaaa aaggaattct atttttgact
    22381 acacctattt ttacaagaat gctttcaact gcagaatatg gtaagtatac gatttttata
    22441 tcgtggtatt ctattatagc aattttcaca acgttgaatt tatctgcagg tgtctataat
    22501 aaaggactaa ttaaatacga gaataaatat gcattaacct cgtcattttt aggattatcg
    22561 tcatttattt ccctttgtgc attgatgcta taccttccat ttagaacggt tataaatggg
    22621 ctactggatt tatcgacgga gtttgtcata ttgattctta ttgaatcaat atttgagccg
    22681 gcttttttgc tttggtcagt taaggaacga tttgattaca agtatcaaaa actagtactc
    22741 atcacagttc ttatggctgt tttaaatcca ggtctcggta ttatattaat cttatcattt
    22801 ggggagtatg ctatttggag agtacgagct tatgtcgtgg taaatgttgt agtggggatg
    22861 tttattggca taggaatact ggctaagggt aagaaattat ttgataaaga gtactggaca
    22921 tatgcgctca gatttaattt gccattaata ccacattatt tatcaatgac attgctgaat
    22981 caggttgatc gccttatgat aaatagtatg gttggagcta gcgaggctgc gatttatagt
    23041 atcgcttaca ctatatctag tatgatgaat atcataacaa cagctataaa cggttctttt
    23101 attcctttta catacaaagc aattaaagat ggcgtgtatg aaaaaattaa atccgttgct
    23161 aatcaactat tagtgttgat ttttgtcttg tgttcgttta ctatgctgtt cgggccagaa
    23221 ttgatttggg ttgtcggagg agaaaaatat atggatgctg tttggattat tcctccagtt
    23281 gcggcatcag tatttttcat gtttttatat ccgctttttg gcaatattga attttactat
    23341 gaaaagacaa actacgtaat gtttgcatct tgtgtgggct ctatcgcaaa aattatttca
    23401 aactatgtta gtattccccg ttttggatat atttcggctg gttacacgac attgatttgc
    23461 tatatgttgt tttcctttgc ccactacatg ttttataaaa aaattcttag agaaaatggt
    23521 ataaaagaag atatgtataa tattaaaaat attatatttc tttctgtctt aatgattgtg
    23581 ttaactattg gtatgacaat attatacttt tctacaattc taagatatag tattattatt
    23641 ggattagtta ttgtggcttt tcttaaaagg acgcaaataa tgaaaatcct taaaaatata
    23701 aaaaattaac aagcaaatct tgggaatgga acggaggcac ggtgaaatga gattattgat
    23761 tccatctgct aagattgtac cggaagaact tcaccatctt ggaaaacttc ctgcaattat
    23821 ctatccgatt aatcaaaaaa ttgtatttga ttatttatat gatcaatata aagatgtatg
    23881 ttctgctatc gatattgcct gttacgaaaa gatggataag gtagcaagac gattagataa
    23941 gtatattaaa tctaaaacag taaatattat tcagcttaaa gagttagggg atcttggaag
    24001 aactatatat gattctctaa taggatgtga cgagcctgtt attattaatt ttgctgatac
    24061 cattatcaat gataatatat attccttgga atgtgacagc tttttctatg ctgaagacta
    24121 ttatagtaat acttggacat tctttgaaga aaaagacgga gatattattt ctgttttaga
    24181 taagaacgag ttaaaagaag atgatggaaa gaagcacaaa ctgttttctg gtgtatttca
    24241 aattatggat gcccagtatt ttagagagtg tttgcgtaaa gctttgatgt caaatgtagt
    24301 aaatgtgaat agtttttatc aagctttaca agagtatagc aaacgatatg aatttctctc
    24361 tattaaaaca aataattggt ttgacattgg acatgcagat aaatattata attccaaatt
    24421 ggaagttaag gcaagagaat ttaaccacat ttcaattgat aaagatcgta gcatcctgag
    24481 aaaaattagt gaagatgttg aaaaatttat cggagaaatt aagtggtatc ttaagttgcc
    24541 agcacaggta gaatatgtga gacctagaat attcgaatat tctacttcgt atattaatcc
    24601 atatgtaagt atggaatact attcgtatca tactgttcac gagttatttc tctatagtga
    24661 cttaacaaaa aaacaatgga tagatatctt taatagaata cgatttgtat gctcagactt
    24721 taagagatac tctgtttcag gggataatat tcaaaagtca ttgaaagata tgtatctcga
    24781 taaaacattt cagagattca ataagctcag aaaggacccc cggttcacag aattcttttc
    24841 ttctgatatt caaataaatg gagtcagata taaatctttg gaccagatag aagctttatt
    24901 gagcgtaagt gttccaagag aattgtttga cattacgcaa tttaatatta ttcatggtga
    24961 cttatgcttt gccaatatta tggtagataa cacattttcg ttcattaaag tcatagatcc
    25021 gcgaggtaag tttggagatt ttgatattta tggtgattat agatatgaac tggcaaaatt
    25081 gtttcatagt gttgatggaa aatatgactt tataattaag gacttgttta caataaaata
    25141 tgatccaaag aaggcaatca tagactatat tgttcaagat cgtaaacgag attatgattt
    25201 atatgaagta ttttactctg tcttcaaaga tgaaataggt tctgatttga aaaaaattga
    25261 acttattgag gctttgctgt ttttaagtat gattcctttg catggagaaa gcttaaacca
    25321 tcagatggct atgttagcaa ctggtttgga aattttagga cgagttgtcc cggatattta
    25381 ttgttaatgc tttgagctat tgtttttcta tttgatcagt gtaaagccgg gcatataaat
    25441 tgcctggcta aaataaagga gatgtcttag ttatatgttt aaagattacg catttgtttt
    25501 tgatattgat ggaactttgt gccccatcaa aaagaaagat gagaaatatg aagatttagt
    25561 tccgtatgct aatatggtgg agaagttgcg atactataag gagaacggag caaagattac
    25621 actgttcaca tctcgcaata tgaattctta tcatggtaat ttaggtgtga tcaatgcaaa
    25681 tactgcaaaa atcttgttgg cttggctaga taagtgggag attccgtatg atgaaattct
    25741 ttatggaaag ccttggccag gccataaagg gttttatgtt gatgatcgca cagttcgtcc
    25801 ggatgaattt ttatcgtgta ccctggatca gctcgatgag atttgcaaaa aatccaaatt
    25861 gaaatctgag taaaggagac tttataaagt gaataagatg acggttatta ttacaatggc
    25921 tggtcttggc agtcgctttc gtaaagttgg ctacactgtg cccaaattta tgattgaagc
    25981 aaaaggtaaa accttatttg attggtcctt agacagcttg cttgattata attcacatgt
    26041 agaaaagtat gtttttgttg tgcgtaagga agatgatgct actgttttta tcaaagaaca
    26101 ttgcagtctg tatggaattg aaaatgtcga ggtagttgaa attgactata tgactgatgg
    26161 acaagccact acatgtatgc ttgcaattcc ttattgcgca cctgataatg caattatggt
    26221 ttataacata gatacatatg ttgagccagg tgagatgaaa tacgttgata tttctggaga
    26281 aggacatatt ccgtgtttcc atgcggacgg tgatcattgg agttttgcca agttgaacga
    26341 atatggtgat gtagtagaag taagagaaaa agttagaatt tctaacaatt gtactttagg
    26401 tgcatattat ttttcttctg caaaattata tgcatcactc tacgaagaat attatgcaga
    26461 tgaaaggcat ctggaaaaga atgagaaata tattgcgccg ctatataatt atatgataga
    26521 gaagggcatg aaggtaacca ttagtatagt agataaatca aaagttcacg tactcggtac
    26581 tccagaagag ctcgatgtgt tcttaaatga atagggaaaa tataatcata gaaaatgtta
    26641 attataaagc tgcgtgtgtg ggggagtact attcgataga tattctaaaa atactaatgg
    26701 ctattttagt tgttgttcgt catttaggtc aaaactattt agctccagat taagcattta
    26761 gtattggtat tgttgcagga ctgagtacgg ttgccgttcc tacttttttc atcatcagtt
    26821 cctttttgtt gtgtagtggt gaagttacat ccgctaaatt atctaaacaa atctacagag
    26881 tacttaaaat ttacattatt tggtctatat tatatattcc attagtatta aagaattggg
    26941 tacagtcaga ttttagctgg aaaacagatg tattattgta tatcagagat tttctgttta
    27001 ttggtagtta ctatcacctg tggttcttgc ctgcactggc ttttgctcta tggttagtac
    27061 gcaaaagcca ggtttctaaa ataaagtttc tcagtggttt gttgctatgc gtagtaggtt
    27121 tagcatttga atcttattgg tttatattat ctgggaatga ttccatcgag tatattagat
    27181 attgttataa aagcgtgttt gtaacaactc gtaatggatt atttataggt gtaccatgcg
    27241 tcattattgg gcaacactta gccttccaca aaaacactat acttaataag gtctttaaat
    27301 accgtaattt tatcttatgt gctgttttag ctataggatg cttattagtt ggtgaaagtt
    27361 tcatcataaa tcaaatagct ttgcgagaag tcaataattt gttaatattt tccattccat
    27421 tagcactggt agttgtatca gtatcactga gcctaaagat agtacattta taaaaataac
    27481 agtacatctg cgaagaatta gtacgttatt attttgttta catccgtata taatagctgc
    27541 tttaaatttt attttcccaa aattggggat tacaaattca ttaatagaaa tgacttgtgg
    27601 aatgataata attttaattt ctacagtttt tgtgtatgcg tgtagtgtga aatttacctt
    27661 gctgaaaaaa ttatattaga ttagtaactg gtacatgtaa atttgttgaa attatagcag
    27721 catagagata ggaattctgt tgagttttat tttcgctggc cgaggttatt catgttagag
    27781 tgggtacctc cttttacgaa accttcttga tggtactcat ttagcgcatg atgaaaggag
    27841 agtttatgaa gaaaatcata tcttctattt tggttaatgt gttatctgca ttgtatcaaa
    27901 tatttgggtt tcgattttaa ttgcagtttt ggctatgttt ttatacttgt ttgcgatggc
    27961 tgaggagaaa tcaggattag gtttgaaaaa atcttttctt gtctggattc ggtatttcag
    28021 aaaatcaagc tcatttagaa aaatgttttt gcttgtgttt tttacaaata tgattctttt
    28081 tagaacactg atggatagat atgtttggct aaatcctttg tctaatgtgt ttggtagttg
    28141 gtggatttac tctgtgaatg aaacgacagg ggagattaga ttaactacag aatgttttga
    28201 aaatgttatt ctgtttattc catttataat tctagtaatg tggtataagg cttcagaaaa
    28261 agattttgga aaaatttatc agagattata aaatttgttt ttttatgttc gtgtcttata
    28321 gagtttttgc agttattttt acacatagag acattccaat tgtcagattt attttataat
    28381 actttgggtg gcggcattag tggggttata tattatttga gctgatatat tcatcctaac
    28441 cttaaaaata aatgagcaga attttaaaag attggagaga ttctatggat cataatgcaa
    28501 attattatca ttggcttaca aatgccaatg cagaaattgt agaggaactg cggtcatatt
    28561 ccgagaagga cattgaggac tccttctata aaaatctgtc ctttggtacc ggtggtctgc
    28621 gtggcaccat tggtgcaggc actaatcgta tgaacgtaca caccgtaggt aaggcaagcc
    28681 agggactgtc agactatctg aataagacct atgaaacccc ttctgtcgtc gtgggctatg
    28741 attcccgaat caagtccgat gtatttgcaa aggttgccgc ggacatcttt gctgcaaacg
    28801 gtattcaggt gcatctgtgg ccggagctgc tgcctgtgcc taccgtatcc tttgctatcc
    28861 gttatctgaa ggcttctgcc ggtgttatga tcaccgcatc ccataatcct tccaagtata
    28921 acggatataa ggtctatggc gcaggcggct gccagatcac caccgaggct gccgctgaga
    28981 ttctggctga aattgaaaag ctggatattt ttgcagatgt gaaaatgggc ggcgacgctt
    29041 ccagaatcgc ttctatccag ccagaggttc tgactgcttt cattgaagaa gtaaagggcc
    29101 agtcggttct gtttggcgaa gaagtggaca agaatgttgc tatcgtgtat tctcctttga
    29161 atggtaccgg tctggtgcct gttacccgta ctctggcaga aatgggctac agcaatgtga
    29221 ccgttgtcga agagcagcgt ctgccagacg gtaatttccc gacctgtcct tatccgaatc
    29281 cggaaattaa tgaagctatg tctctgggca tggagtatgc caagaagtat aatgctgatc
    29341 tgctgctggc taccgaccct gactgcgacc gtgtgggtat cgccgtgaag aatgcagaag
    29401 gggattatgt cctgctgtcc ggtaacgaaa ctggtctgtt gcttctggac tacatatgct
    29461 ctcagagaat taagcacggc aagatgccca ctgaccctgt gatggtcaag accatcgtga
    29521 ctatggatct gggcgagcgg atcgcttcca actatggcgt gcgtactgtc gatgtgttga
    29581 ccggcttcaa gttcatcggc gaacagattg gtatgctgga aaaggcaggc aaggtggaca
    29641 gctatatttt cggctttgag gaatcctacg gctatctatc cggcagctat gtccgtgaca
    29701 aggatggtgt caacgccgct tatctgatct gcgagatgtt ctcttactac aagactcagg
    29761 gtatcagtct gtttgataag ctgaacgaga tttatgctaa gtatggctac tgtttgaaca
    29821 ccctgcattc ctttgagttt gagggtgcgg caggttttac caagatgcag aaaattatgg
    29881 ttgaattcca taaaggtctt gatatgattg gaggtaagaa ggtcattgaa accttggatt
    29941 acagcacggg actgaatggc ctgccgaagt ctgatgttct caaatatctg ctggagggca
    30001 actgctctgt agtggttcgg ccttccggca ctgagccgaa gctgaagacc tatatcagcg
    30061 ttagcgcaga aagcaaggaa gctgccgaag taatggaagc tgttatggct gctgaactgg
    30121 ctgaaatgtt taagtaatac tatgaaaaag atctgagtgt caatgtccga agtagcaaca
    30181 tctatgttta ataaaaattt cctgctttac ggttctgttt acataattga ggtaaagggg
    30241 ccgactgtgt ggatcgaaaa ggcaaagaag tgttaacaga gacacagcag cctgatgaag
    30301 caccgatcaa agcagtaagg gagaaataag agatattaga gttttaataa tgtattcagc
    30361 aatagagatg aaaggagtag aatatgaata aaactattga aataataaaa tgagggggaa
    30421 agaaataaac gtgatattat tagttttgag aaggcgtttt caatattcgc tattgtattg
    30481 atgtactatt ttcagcaatc gtatttgcca gcaatactga acaaagcaat atctattggt
    30541 gatacaggag tgcatatatt cttcttttgc tcaggttttg ggctatatct gtcatattta
    30601 aaaaagccta ttacattggg tgaattttta cagaaacgat ttatcaagat ttatattccg
    30661 tatattatcg taattacggt atcagtgtta ctaccttata tgacacaaga tttaaatgct
    30721 gcaaaagtat atttgtctca tgttttgtta tttaaaattt ttctaccagt atatgaggag
    30781 tcactgggtc cctttggttt atttcgacaa ttattcagtt ctatttctta ttcataccaa
    30841 ttgtaaaact atagagaaaa ataggaatga aatgattgct tgttgcaagc tgagttatta
    30901 gtatctgggc tattatgatg tttcatacag gatatggaag catacgagtt cttgggagct
    30961 gctttgcgca atatatttgg gaatttactt tcggcatgtt tggttgcaga gtatttagtg
    31021 aaaagaaaaa tagtaagatt taataatata ttcgtgatta tggcgtccat tgttggaatt
    31081 ggttttgctg gtatatcgaa aattattggt ggactgatgg agtctattaa tgatattttt
    31141 gcattcttcg gttacggtgc aattgcaatt ttaatgtatc aagttattcc atttagaaag
    31201 ataatgccta ggatttcaag atactcatat gatatatatc tactccatat tcttgtattt
    31261 agtacgattc atcaaattat ccctaacttg gttttggcgg catttgtttc cattcctata
    31321 acgttggtgt tgtcatatta ttatagtatt atgttatata gacttaaatt aaaatgatag
    31381 cataattgtc gcctctaccg ggcggttgcc atcctgatta acagcctcca cgcggcggat
    31441 ggtgggcact taggtagttc gtaacctagt ggtagatggt tggtaaaaag gagataaaac
    31501 taaatgatta
//
LOCUS       DACEDM010000033        31308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEDM010000033 DACEDM010000000
VERSION     DACEDM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.033"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IWO50_001340"
     CDS             complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IWO50_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0358471.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(419..2725)
                     /gene="infB"
                     /locus_tag="IWO50_001341"
     CDS             complement(419..2725)
                     /gene="infB"
                     /locus_tag="IWO50_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0358472.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2738..3049)
                     /locus_tag="IWO50_001342"
     CDS             complement(2738..3049)
                     /locus_tag="IWO50_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0358473.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3046..3339)
                     /locus_tag="IWO50_001343"
     CDS             complement(3046..3339)
                     /locus_tag="IWO50_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0358474.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IWO50_001344"
     CDS             complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IWO50_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0358475.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IWO50_001345"
     CDS             complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IWO50_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0358476.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5177..9529)
                     /locus_tag="IWO50_001346"
     CDS             complement(5177..9529)
                     /locus_tag="IWO50_001346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0358477.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9741..11450)
                     /locus_tag="IWO50_001347"
     CDS             complement(9741..11450)
                     /locus_tag="IWO50_001347"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0358478.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IWO50_001348"
     CDS             complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IWO50_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0358479.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12947..13747)
                     /locus_tag="IWO50_001349"
     CDS             complement(12947..13747)
                     /locus_tag="IWO50_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0358480.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13744..14556)
                     /locus_tag="IWO50_001350"
     CDS             complement(13744..14556)
                     /locus_tag="IWO50_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0358481.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IWO50_001351"
     CDS             complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IWO50_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0358482.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15311..16033)
                     /locus_tag="IWO50_001352"
     CDS             complement(15311..16033)
                     /locus_tag="IWO50_001352"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0358483.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16169..17050)
                     /locus_tag="IWO50_001353"
     CDS             complement(16169..17050)
                     /locus_tag="IWO50_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0358484.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IWO50_001354"
     CDS             complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IWO50_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0358485.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18290..18769
                     /locus_tag="IWO50_001355"
     CDS             18290..18769
                     /locus_tag="IWO50_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0358486.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18986..20077
                     /gene="phnW"
                     /locus_tag="IWO50_001356"
     CDS             18986..20077
                     /gene="phnW"
                     /locus_tag="IWO50_001356"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0358487.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20070..20855
                     /locus_tag="IWO50_001357"
     CDS             20070..20855
                     /locus_tag="IWO50_001357"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0358488.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(21201..22892)
                     /locus_tag="IWO50_001358"
     CDS             complement(21201..22892)
                     /locus_tag="IWO50_001358"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0358489.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IWO50_001359"
     CDS             complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IWO50_001359"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0358490.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IWO50_001360"
     CDS             complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IWO50_001360"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0358491.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IWO50_001361"
     CDS             complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IWO50_001361"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0358492.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            27176..27877
                     /locus_tag="IWO50_001362"
     CDS             27176..27877
                     /locus_tag="IWO50_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0358493.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(28449..29465)
                     /gene="tsaD"
                     /locus_tag="IWO50_001363"
     CDS             complement(28449..29465)
                     /gene="tsaD"
                     /locus_tag="IWO50_001363"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0358494.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(29462..29926)
                     /gene="rimI"
                     /locus_tag="IWO50_001364"
     CDS             complement(29462..29926)
                     /gene="rimI"
                     /locus_tag="IWO50_001364"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0358495.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(29933..30475)
                     /gene="rimI"
                     /locus_tag="IWO50_001365"
     CDS             complement(29933..30475)
                     /gene="rimI"
                     /locus_tag="IWO50_001365"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0358496.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(30459..31283)
                     /gene="tsaB"
                     /locus_tag="IWO50_001366"
     CDS             complement(30459..31283)
                     /gene="tsaB"
                     /locus_tag="IWO50_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0358497.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT         8491 a         6715 c         5523 g        10579 t
ORIGIN      
        1 aatcccgcaa cccctgattt cacaggattt gcgggatttt ttgattagtc tttgttcaag
       61 tcgcgaagca attcatcgat atggttccca tagacaactg attcatcaag ttcaaagaac
      121 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
      181 gctttattca atccatcttg tgcttttttc ttatctgatg ctaagtcaga taaaatgctg
      241 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
      301 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
      361 tgaccgactc ggcgatcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
      421 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
      481 tgaatttttc aatcatcgca ccacattcat aacccatttt gacttctttc acgtcatctt
      541 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
      601 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
      661 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
      721 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
      781 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
      841 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
      901 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
      961 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
     1021 cttctttcaa ttcgccttct ttcaggcttt caaataagtt gtccaaagtc acacggcttg
     1081 tggcagcacg gttttccaat aatgcacgtt ttgcgcgttc ttcacccgct gcacgtgctg
     1141 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
     1201 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
     1261 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaacg tgaagtgttc
     1321 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
     1381 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
     1441 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaaa
     1501 tcgtttctcc accccatgct tcaggaatta attcatactc acttaattct tgcataacat
     1561 gctgaggatt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
     1621 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
     1681 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
     1741 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
     1801 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgcaatgtat
     1861 ccaataaggt tgttttcccg tggtctacgt gtcccataat cgttactact ggcggacgtg
     1921 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
     1981 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
     2041 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
     2101 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
     2161 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
     2221 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
     2281 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
     2341 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
     2401 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
     2461 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
     2521 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
     2581 cttgttgcaa tttcttctca tcactgctag taatcgcacc catatggttt ttcacatcga
     2641 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
     2701 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
     2761 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
     2821 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
     2881 cttttatctt ttttcttgta ttctcacttg catcggttgc cacaaacacc aattttgctt
     2941 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
     3001 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
     3061 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
     3121 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
     3181 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
     3241 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
     3301 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
     3361 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
     3421 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
     3481 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
     3541 agtcaattta gcagctaaac gtgcattttg tccgcgttta ccgattgcta gcgataattg
     3601 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
     3661 gacttgtgca ggattcaatg catttgcgat gaatacagct ggatcttcat tccattcgac
     3721 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
     3781 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
     3841 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
     3901 tacttcttgt tcaaataagc gacgcaatag gtctggatgg ctgcggctaa caaatacttg
     3961 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
     4021 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
     4081 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
     4141 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
     4201 ttgtttagcc gtttgcgcag cgatccgtcc aaaatctttt ggtgttactt caaaacggat
     4261 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
     4321 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
     4381 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
     4441 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
     4501 taaagcgtct aacgcattta acatttcttt actcattttt ttctgattcc tctccgtgat
     4561 taaaattgga tagctaagcg agcttttgca atattttttc ggtcaaaagt caattctttt
     4621 tctcgtgttt ttatgcgtat tttcaatgta agctgttcag catcaaatga ttgaagaaat
     4681 ccttcatatt gtttttcgcc atctactggt tggtataagg agacatggac ataatcacca
     4741 agtgcctttt catagtcgct ttcttttttc aatggccgct ctgctcccgg ggaggatacc
     4801 tccaggaaat aagcttgagg aatcggatct ggatctgctg catcaagttt ttcactcagt
     4861 ttctcactga caaaggcgca ttcttcgata tcgataccgc cttctttgtc aataaatact
     4921 cgtaagaacc agctttttcc ttcttttaca aattcgactt cgacaagttc aaaattttgt
     4981 tcatctaaaa tcggtgtcac catttctgtg actgtttcaa cgacgctact caaaagtttc
     5041 gcctccttaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
     5101 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
     5161 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
     5221 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
     5281 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgtttcg
     5341 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
     5401 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
     5461 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
     5521 ctaatccctt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
     5581 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
     5641 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
     5701 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
     5761 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
     5821 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
     5881 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
     5941 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
     6001 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctaa catccctcga acaaaacgag
     6061 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
     6121 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
     6181 gtgggtcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
     6241 gaatatctaa tttcaataca ttatcgtgaa tcgagtggaa atcaaaatga gtcgttttcc
     6301 attctgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
     6361 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
     6421 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
     6481 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
     6541 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
     6601 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
     6661 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
     6721 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
     6781 gacaataata atgaggggct aatggattaa cttcagtgat tcctgtcatc gtcgcaacaa
     6841 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
     6901 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
     6961 ctttttctaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
     7021 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
     7081 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
     7141 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
     7201 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
     7261 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
     7321 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
     7381 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
     7441 taacttcaat ataatcatag aattttgctc ggtttcttgc ctcttctacc cctttttgca
     7501 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
     7561 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
     7621 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
     7681 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
     7741 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
     7801 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctaacacca aactttttag
     7861 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
     7921 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
     7981 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
     8041 acatccgcaa tacttcttct tccgactttg accctcgaac catttcatcc gtgataccag
     8101 tcagatctac cgttgttcgt gacaatggat gtcctggatc gataaattca tcaaagcttt
     8161 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
     8221 cagacaatcc tgtcgtttcc acgtcaaata cgacgtatgt ggcttcactc aatgcttcat
     8281 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
     8341 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
     8401 catggtctgt aatggcgata gctcggtgtc cccattttcc tgcttgtgcc acaagatcag
     8461 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
     8521 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
     8581 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
     8641 agctaccgac actgattgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
     8701 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
     8761 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
     8821 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
     8881 tgggtccttc aagagctggc gattgctctt ttctttcctt tttcttttgt tcgtggacaa
     8941 ctagactttc agctgcttgc ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa
     9001 aaagtttcaa tacacgttcc gcttgctgct catccacttc tggttcgata cgaaattttg
     9061 ggaatccgta actgacgaac aactcttcta ctaatggcaa gtattgttgc ttcaaataag
     9121 aaatagctcc tgtactgtcg attggcaaga tgactttacg atcttttatc acaggtacct
     9181 gcgttttcaa tacttgctgt gctagtggtg tatcgcattg ctgattttcc aaagcttggg
     9241 cccagtaatc ttgcaagagc tgttcatcga atgtctggtc atctgctaag atattcagtt
     9301 tgacttgtgc aatatcttta aaagcgatag tcagatgctg agtcaatgac cgatatagca
     9361 taataggcaa aattttggaa aaatgtagcg taaattccca taaacgcgat ttacgatgga
     9421 ccactacact atccatttca ccagcagtaa gcagtggatg atttttttct gcttcctcta
     9481 gttggatttg ttccaacagt ttttcaaata aatctcgttt ttctgacaaa gatatacgcc
     9541 ctttctgatg agaaacaatg caattgatcg catcttatcc tcttcttttt cagtaccttt
     9601 tagtatactc gttttttctg agttttacat taaaaaattg gaactgcgat tcaaatcgca
     9661 gttccaattt taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag ctatttttat
     9721 tttctgtgtt ttaacgctta ttattcgcct tggttcaaga ggattggtaa agtattcgcc
     9781 aattcttctt tacgtacttc aaccatttcg ccagttttct tgattttcac ttctacgatt
     9841 ccatccactg cttttttacc gacagtgatt ctgattggac agccaatcaa atcagaatcc
     9901 gcgaatttca ctcctgcgcg ttcatttctg tcatcaacca gtacttcata gccagctttt
     9961 gtcatcgctt cttctagttc attcgtcagg cttgtttggt attcgtcttt tagattcatt
    10021 tggacgatat gcagatcaaa tggcgcaatt ccttttggcc aattgattcc ttgttcatct
    10081 gaatgttgtt ctacgattgc tgataataaa cgacttacac cgataccata acagcccata
    10141 atcacaaatt tttctcgtcc attttcgtct agtacagttg ctcccataga ttcactgtaa
    10201 cgagtgccga ttttgaatat atgtccgatt tcgattcctc gagtaaaggc aagaacaccg
    10261 ttgttgtctg gtgaaggatc tccttcttgt acaaaacgaa gatcttcata agaaagcaca
    10321 gtgaaatcgc gttttggatt cacattttta tagtggaatc ctgtttcgtt cgctccagta
    10381 atggcattcg ctaaatcttg gacataacgg tctgcatata cttttacgtc atctgaaaca
    10441 ccgactggtc cgacagaacc aaactcagca cctagatatt tttgtgcttc ttcttctgtt
    10501 gcttcttcta aaaagtcagc agataaaaag tttttcagtt ttacgtcatt gacttcatgg
    10561 tctccacgta ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac agacttgatg
    10621 attttttgag gttcaacaga aaagaaatct gctacttcgt caattgattt tacgttaggt
    10681 gtagctactt tctccaattc aagctgtgtt tcatgcgatt tttttgatac atagtagctt
    10741 gtagccatct ccaagtttgc agcataatca ctttcagtcg aataacagat cgtatcctcc
    10801 ccgatttctg agatcgccat gaattccttt gagtctttcc cgcccattgc gccgccgtct
    10861 ccgatgatcg agcgaaattc taagccacaa cgcttgaata cttcggtata ggctttttcg
    10921 taatctttat atgtctcgtc caaactttct tccgttgcat ggaaagaata tgcatctttc
    10981 atgataaact ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc acgatattta
    11041 ggttggattt gataaagatt taaaggtaat ttcttatatg aattgatttc attacgcatc
    11101 aaatcagtaa aagtttcttc gtgagttggt ccaaggatca tttttcgatc attgcggtct
    11161 ttgaaacgat acaagtttgg tccatatgtt tcgtaacgcc cggattcttc ccaaagttct
    11221 gctggcaaga tggcaggcat cagcatttct accgctccga ttttttcaaa ttcttcccgc
    11281 ataatgcgtt tcagtttttc caatacacga tttgctaatg gcagatatga ataaataccc
    11341 gctgatactt ggcggatata tccagctctt agcaagattt gatggcttaa tacttctgca
    11401 tcatttggaa cttcgcgtaa tgttggaatc aacatttttg attgtctcat tctatatact
    11461 cctttatttc ctcaaaatag ttatcgtttg ttattatacc ctatttgtca actttttcta
    11521 gaaaaagaat cgttgaatat cgttccatgt aactagcacc atcaacaaca ttaaaaatcc
    11581 aaagccagct aatgtcaata ttccttcttt ttcttgactt aatggtttcc cgcgaatccc
    11641 ttcaaaaata ttcaagacta atttcccgcc atctaaggca ggaatcggaa gcagattgac
    11701 gattccgaga ttcattgaaa gaagcgccat caaactaata acagttgtta caccttgatt
    11761 tgctgcttct gaagacaatt ggtacatcat gacaggtccg cctaatttat ctaaactgaa
    11821 acctgtaaat agtgatccta aggctttgaa aatttccaat gaaccgctga aagcttgacg
    11881 cgtccctcca atgatcttat ccataaaacc tgtattcatt ggggctttta taccaagttg
    11941 gccaactttt tcgccatttg actctacaga tttcggtgtc acgtcgacag attgcacctg
    12001 accttctcgt tcgatcttaa agtctaatgt tttacctgga tttttcgtaa tgactgttgt
    12061 cagatcattc catgaatgga tttcttttcc gtccacactg actacctcat cattttcttt
    12121 taagcctgct tctgccgcag ctccattagg catgatctct cctacgcgat tagtgttcgt
    12181 cacttgaaca ccaccttgca taaatgccag aatgatgaat aacacgattg ccaaaataaa
    12241 gttattcatg ggtccagcaa agttggtcag catacgctgc cacaatttag ccgattggaa
    12301 ctgtacgtct tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc
    12361 gtgatcaaca gggtaggtta ctacttctgt ttcgtctcca ttcacataac cagtgatagt
    12421 cagctcatct tctaaatcat aacggctcaa ttccatcggg atagcatttg tcaattggat
    12481 tttcttactt aaatttatct tctctacgat gccgtcagaa tttagtaaaa gagaaagagg
    12541 cattcctggt gccatttctg tttcatcatc cccattgcct gccattcgaa cataaccgcc
    12601 aataggtaat aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc cataaatttt
    12661 cggacccata ccgatagcaa attcgcggac gaggattcct gatcgtttcg caaagaagaa
    12721 atgaccaaac tcatgaacaa tcactaatat accaaaaacg atgataaatg tcagaatcgt
    12781 tttcatagct tttttctcct ttacttgttt tttgtgtttt ttataaaaca acgataaaac
    12841 actataattt taccatagta taccaaagac accaatagta tcaatataat gaaaaataaa
    12901 aacctgcgtc ttttatgaaa ttcacaaaga aacgcaggta tacttactaa ataatgccaa
    12961 ataaatgcat gataggaaat acaaacagca aactgtcgaa acggtctaag attccaccat
    13021 ggcctggtaa gatatttccc gagtctttca catcataatg acgtttgatt gcagattcga
    13081 ccaaatcacc aaattgtccg actgctgaaa aaataatcgt catcagaagc atgacaaaca
    13141 taccatgatt gaatagttct tttgctgggt atagcaataa ataaagaaat gcgactacaa
    13201 cagcactacc gataccaccg agcgcacctt caatcgtttt gttgggcgaa atatcaggac
    13261 ttaacttatg ctgaccataa cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa
    13321 caataaatag accaaatatc aaaacagcca agctttctgt tcgtgcattg acaaaattct
    13381 ggaaacctat tcccacatac aagctgacaa gcacaggaaa acctgcttca tcaatcgtat
    13441 acgtattttt agaaataact gatgttccta aaagaatcat aactataaga taaaataaca
    13501 taaatgtact tacattctct ggaagaaaga aaaaccatct ttcttttggc aaaacaagta
    13561 acactgcacc aacggcggat aaaactcctt caaaacttaa gagtgtcaat cctttcatcc
    13621 ggaacagctc gtaaactcct actaccgcta agattgctgc tgtaagctca accgctatgc
    13681 ctccgatcca tataatcgga ataaacaaga ctagcgccac agctgctgtg atcacacgtt
    13741 gtttcatgac tgatcccctt tctcatcttc tgtttttttt acaccgccga atcgacggtc
    13801 tcgattttga taagaggcga ttgcttcttc aagatgcgca ccatcaaaat caggccaaag
    13861 cgcttttgta aaatacagct cactatatgc gatctgccat aacagaaaat tactaattcg
    13921 ctcttctcca cttgtacgga tcatcagctc aggatcttgt aactctttag gcaagaaacc
    13981 tgtcattaaa tgcttagcaa tcaattcatc atcgatttct tctgcgtgta tttctccttt
    14041 tgctacttct tcggcaattt cttggacagc agtcacgatc tctgctctac ttccataatt
    14101 caaagcaaaa ttcaatacca ttccagtatt gttttttgtt tgctcaattg cacgtcttac
    14161 agcatcttgc gtatgttctg gcaatacgtt ttcataaccc atgacatgaa ctttgacatt
    14221 ttctttaatc aattctggta cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa
    14281 acttacttca tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac
    14341 tttcactcca agccttgatg ctttttttgt gactttcttt actgtttcca tgccttcttt
    14401 atggccagca actcgtggca aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat
    14461 gatggcaatg tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc ttttttcttg
    14521 aatatatttg tttttttgcg gaaaaaaacg taacattctt tttcctccta tacttaaaag
    14581 attcccacta tcttaagtat tatagcaata tctaactgtt tttcctatga aatcattgtt
    14641 tttttattta aaaactgaat aattttttct ttttttaatt tttaaataaa agaaatataa
    14701 ataaaaacag aggaagcgga atgatccgcc cctctgtttt tatgatttta ttaaacttcc
    14761 aataattctt tttctttatc agcagcgatc ttatctactt ctttaatgct gttatctgtc
    14821 aatgtttgta cgtctttttc taatccgcgc aaatcatctt cggtaatatc accatttttt
    14881 tgttgttttt tatattcatc gatcgcatca cgacggatgt tacgaatcgc gatttttgca
    14941 ttttctgctt cttttttcac atctttagct aattcttttc tgcgttcttc tgtcagttgc
    15001 gggataacta agcgaatgac atttccatca ttggttggac tgattccgat atcacttgcc
    15061 ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca
    15121 cgtgcttctg gaatttggat tgatgccatt tggttcaatg gtgttggcgc accgtagtag
    15181 ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt
    15241 tcacgttgaa gattttgtgc tgcttttttc attttttctt ttgcttctgt cataattgca
    15301 tctgccattt ttatttcccc cttacagttg ttccgatatt ttcgcctaag attgcacggc
    15361 gaatatttcc gtgttcattt aaattgaaga caagcagtgg aatatcatta tccatgctta
    15421 gtgaacttgc agttgaatcc atgacttgta atcctttggc aataacttcc agatgagtca
    15481 gttcttcaaa ttttactgca tttgcatcta ctttcggatc agcagaataa acaccgtcta
    15541 cattgttttt agccatcagg ataacatctg cgccgatttc tgccgctcgt aatgctgctg
    15601 ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc aaagataacg atccgtcctt
    15661 tttctaaatg acgttctgct ttacggcgaa tatatggctc tgcgatttga cgcatttcaa
    15721 tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt aaagctaagg
    15781 cattcataac tgtagctaac atacccatgt aatcagcttg agcacgttcc atgcccatat
    15841 gtgctccgat ttgtccgcgc cagatatttc cgccacccac aacgattgcc atttcaacgc
    15901 ccagctcgtg aacttctttg atttcttgga tgatttcttt gattactggt ggtttgattc
    15961 caaatcctgc atccccagct aaagcttctc cactcaattt taagactaca cgctgatatt
    16021 tcggtgtgac catgttcatt cctccaatat tttctattgc cattttacca tacttagatg
    16081 atttcgacac tctaagactg gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc
    16141 gtgcgttccc gtctgttatt caacagaatt attttttcac ttggctcata acttcttcta
    16201 caaagttatc ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg aatgttttaa
    16261 cagttgcgcc ttttgatgca acgtattttt caacagtcat atcaggatct ttaacgaatg
    16321 gttggtccac caaagcgatt tctgctttga atttcttcaa gcgtccttct accatttttt
    16381 caacgatatt cgctggtttg ccttcgttca atgcttgttc tgtaagaaca gttttttcat
    16441 gttctaattc agcttctgga atttgtgatt cgtttacata acgaggattg atagctgcta
    16501 cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt tccatctaga acagtcaata
    16561 cggcaatacg tccgcccata tgcaagtatc caccaaatgc agcattgtcg tctttttcta
    16621 caacttcaaa gcgacggaag ctgatttttt ctccgataac ttgagttgct tcgatcaagt
    16681 cagattcaat tgtaccttta tctgttttga ttttcattgc agcttccatg tctgctggtt
    16741 tgttttcagc aacaagttct gcaatttctt ttactaaatc ttggaacatt tcgtttttag
    16801 aaacaaagtc tgtttctgag tttacttcaa cgattgctgc agtgttacct ttcacagcaa
    16861 ctgaagccaa tccttcagct gcaatgcggt catttttctt cgcagccttt gccattcctt
    16921 tttcacgtaa aagatcaact gctttttcca tgtcgccttc tacttcaact aatgcttttt
    16981 tcgcatccat cattcctacg ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa
    17041 cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg
    17101 agagtgaggc ttgtcgccca gcctttgaaa cagctccatc attcaaaatt attctgctga
    17161 agaattgtcg ccttcaacaa catctacgat ttcttcgatt gatgttgctg tgttttcttc
    17221 tgtgaaatct tcttcaacta cttgatcttc accttggttt ccttcgatga aagcatcagc
    17281 catttttgaa gtaatcaatt taacggcacg aattgcatcg tcgtttgaag ggattactac
    17341 gtcgatttca tctggatcgc agtttgtatc aaccatagct acgattggga tatttaattt
    17401 gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca acgatgtaca ttacatctgg
    17461 gattcttggc atatcagcga taccgcctaa gaatttttca agacgttcac gttctttgtt
    17521 caaaccagca acttcttttt taggtagaac ttcaaaagtt ccatcttctt ccattgcatt
    17581 gatttgtttc aaacggctga tacgtttttg gatagtatcc cagtttgtca atgttccgcc
    17641 taaccaacga tggttaacga agtattgtcc tgcacgagtt gcttcttctt tgattgcttc
    17701 ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg ccttcttcag caacgctctt
    17761 catgtaatca taagctgcat ctactaattt aactgttttt tgcaagtcaa tgatgtagat
    17821 tccgtttctt tctgtgaaga tatatttctt catttttgga ttccagcgac gagtttggtg
    17881 accaaagtgt acgccggctt ctagtaattg tttcatagaa attactgcca tgtttgtttc
    17941 ctccgatttg gttttatttt tcctcttctt atcttctacc ttgccggcaa actttttcaa
    18001 gcacctaccg acaatccaat aagaatgtgg atttgctgat gcataaagca ccctcagcta
    18061 ttatacagac aaaagatccg tatcgcaaga gcaaatatac ttttctgctc aacattcaat
    18121 agtttacctg aaagataaaa agaattcaag aaaaacagta gatttctctt tgtttcttta
    18181 ttatcctttt tccataaaat aattttaggt ataaaagacg aatattattg ggcaatcctt
    18241 cttttttttg ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc
    18301 tcaaagacat ttgctgatta aacaagtcat tgaagaattc ccgatccgta cgcaagaaga
    18361 actattaaac aaacttgggc attatggcgt taccgccacg caggcaacga tttctcgaga
    18421 tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt
    18481 gtttcgcgaa aaaggaactc ctgagactag gactgaggaa gaggatcggc tggttcaaat
    18541 gattgaagac attgtattga aagtcgaacg agtccacttt ttgacgatcg tcaatacctt
    18601 gccagacaat gctcatttat ttgcatctgt tttagatgag atcaaacctc cctctattgt
    18661 cagtacaatt gctggattcg atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa
    18721 acgagtagaa gaatttcttc accatccaac tgaagtatca cttttttaat cagagcacgt
    18781 attcctaata tggaatgcgt gtttttttat tttcaatatt ttccttatta aataaacctt
    18841 tacactcttt acaaaaatac atcatttttt attcaatttt ttcaacacct tgattgaatc
    18901 acaataatca tcatttatat tggatgttaa caagagcagc ttcttgctct ttgtgtattt
    18961 caaaaaagaa atgaaaggaa atcatatgta taaattatta actcctggac cattgacgac
    19021 ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt acttgggatc aagaatacaa
    19081 agaaattaca caatggataa gaaaaaaatt attggatctt gctcaagtat cggaaggaat
    19141 atacacaacg gtcttaatgc aaggtagcgg ctcctttggg gtagaatcgg tattaagttc
    19201 tgtgatcagg ccagaagata ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat
    19261 gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat cggaacatca
    19321 aatcctccaa gctcatgtga ttacacagct gattgaagag aatccctcta tctccgcact
    19381 agcaatggtc catagcgaga ctacttccgg tattttgaat aatttggaag agattgctgc
    19441 tgtcacacaa gaaaaagatc ttttgtttat agtggacgcg atgtcaagct ttggtgggat
    19501 accgattcgt gtagccgaac tagggattga ttttttgatt tccagctcaa acaaatgtat
    19561 tcaaggtgtt ccaggctttt cttttgtact tgctaataaa gcggaactag aaaagcgaaa
    19621 aaatgtgagt cgaagtttgt cacttgatct atatgaccag taccgcgtga tggaaaacga
    19681 gaatggcaaa tggcgtttca cttcccctac ccatacggtt ttggcttttc aacaagcgct
    19741 gaaggaacta gaggcagaag gaggaatcga aactcgatat tcacgttatc tcgccaataa
    19801 tcatgtacta cgacaaaaaa tgacagagat ggggttccag cctttcattc aagaatatca
    19861 ggggccattc atcacctctt ttctttaccc taaggactgg cgatttgatt ttaatcattt
    19921 ttatcagtat ttaaagcaaa acggatttgt gatctatccc gggaaaatca gcgatcatga
    19981 atgttttcgt atcggaaata ttggcgagat ctatccggaa gatattttgg aactgacagc
    20041 aattatcaaa gactataagg agaacgttaa tgattaaaac tgtaattttt gattgggcag
    20101 gaacaactgt ggactttggc tgtatggctc ctgtccacgc ttttagaaat gcttttctag
    20161 aaaaaggaat ccaactgact gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg
    20221 atcatatcca acagcttctt tccctccctt ctgtcaaaaa acaatggata gctatttatg
    20281 gacaactacc tcaggaaaaa gaggtggagg aattgtatca acaatttgaa cattatctgt
    20341 ttaaagattt ggtcaagcat tctaccttga aacccgacac attggaaacg atcgaacggt
    20401 tgagaaacgc tgggatcaat attggttcca cgactggatt tactgctcct atgatgacaa
    20461 tcgtagcaga aaccgcaaaa aaagaagggt attcaccaga cttcatggca acttctgaag
    20521 acgtcgaagg gtatggtcga ccgtttcctt atatgatcca taaaaacatg cagtacttca
    20581 aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac ggtctcagat atccaggaag
    20641 ggaaaaatgc cggagtgttg acagtaggtg taattgaagg tagttcgcta atggggattt
    20701 cttatgatga gtaccaaaaa ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg
    20761 ctcttagatt ccaacaagct ggcgctgata tttgtgtaaa caatttaaaa gagttaacag
    20821 aatacatttt gtacgctgac aagaatccag aatgactttt atacaaaatc agaggtcgtg
    20881 aaaaagctgt actgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcatatttt
    20941 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa
    21001 aaagggagct gtgacacgac ttttgtcaca gtccccttat ttacaaacaa acagtgttta
    21061 agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa
    21121 acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct
    21181 gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg
    21241 tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca
    21301 tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt
    21361 gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg
    21421 tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct
    21481 gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat
    21541 tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat
    21601 gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta
    21661 gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat
    21721 tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat
    21781 tttttaccac cttgcgtgat caaaccaaat ggaatatggc gcatatcgtc agaccagtca
    21841 aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct
    21901 actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag
    21961 tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca
    22021 taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct
    22081 aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg
    22141 aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac
    22201 cattgccata attgaatagc ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga
    22261 gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac atataaacga
    22321 agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg
    22381 acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga
    22441 taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt
    22501 cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga
    22561 ttcccgatat tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt
    22621 ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt
    22681 tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt
    22741 cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt
    22801 ggattttcta gtaattgttc gacttgttcc agtgttaaat catctttgac agcgtcatag
    22861 acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt gctcccttga
    22921 ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag
    22981 atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta
    23041 gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg
    23101 gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa
    23161 atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt
    23221 ttctgataaa aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat
    23281 aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc
    23341 ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg
    23401 ttttgcttca acaaatgcaa tagctgcctc tactttcgga agcatgcttc ctggagcaaa
    23461 ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg
    23521 atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc
    23581 cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg
    23641 tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt
    23701 gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt
    23761 aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga
    23821 gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact
    23881 tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac
    23941 atctttatcg atcccttttt ccttcagtac ttcgcccatg gcattttgta accagtaacc
    24001 gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga
    24061 atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat
    24121 gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc
    24181 ttcttgttgg gcttttgcac ttgcatccgt agacaagatt gcattacccc ctaaagcgac
    24241 aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga
    24301 taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga
    24361 tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat
    24421 cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg
    24481 gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc
    24541 gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt
    24601 gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg
    24661 ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc
    24721 tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc
    24781 aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat
    24841 acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg
    24901 accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt
    24961 taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg
    25021 aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat
    25081 tccatcaaac atacgaccta gtactttagc tgtatcttct gtggattctt ttttacctaa
    25141 ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt
    25201 aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc
    25261 tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat
    25321 caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa
    25381 tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg
    25441 aaatcccgac ggcagctgac agtaagaccg ccgtcaggaa acaaattact tcaagtcttc
    25501 acggacaagt ggttggctca tgcagcgtgg accaccacgg cctcgtgaca attcacttga
    25561 attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg
    25621 gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg
    25681 ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa
    25741 tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc
    25801 accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg
    25861 gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg
    25921 gttgtttgga attttgattg ctaacacttt ttcaaatcct gaattttttg cgaataggtg
    25981 gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac
    26041 tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa
    26101 ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa
    26161 catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat
    26221 tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata
    26281 gtcatcgtct gctgacagat cgtatagact gctagaacgt acttcaatat cttttgtacg
    26341 aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg
    26401 aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa
    26461 ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt
    26521 ctcgacgcct tcattttgca atgtctttgc aaagttgtca tgttcttctt gagcaatcgg
    26581 aagatatgga atatcatcga acaacagacg atccataata tctggtgtca aattttccac
    26641 ttcttgtcca ggccttttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg
    26701 aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc
    26761 cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct cacattaata
    26821 ttcgataatt aaataattaa acgtttttct atttttaagc gtaatttatt cattttttat
    26881 tctgtataca ctttcaaaaa aatatttatt cattgaaaaa aattttttga ataaattaaa
    26941 aaaataaatt atattagcgt tgttagcgtt tactatatgt attattgttg ttttttatac
    27001 acaaatcatt cgcttcaaaa caaattattc aataccattg tatatttttt acataataac
    27061 aaattatatt tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa aactttacaa
    27121 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
    27181 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
    27241 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
    27301 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
    27361 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
    27421 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
    27481 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
    27541 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
    27601 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
    27661 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
    27721 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
    27781 agtaatcaaa caactacgtg attccggact gttagattat gacaaaaagc gttttacttt
    27841 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
    27901 cctgatattt atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca
    27961 gttttagatc actgggcaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt
    28021 cactgtttgc aaaaaatact gatagattaa ctagaggaag cgtcttgtcc tcatccgacc
    28081 tagattattc gatattcctc gacaataaac gattaatcag tgagggcaat cagaggaacg
    28141 gataatgatg aataagctgt aaaaaatagg gctttgacaa aagcgtgtca cagcccttat
    28201 ttcgattaaa cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca
    28261 tgaggagctg ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa
    28321 ctcttgatta aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa
    28381 aacatgagaa gccgtttttc ctgggctgtt cttattactt gatgcagggc agatttttca
    28441 caacctcttt attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa
    28501 tttctttttt gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa
    28561 agtcgaagag ggggaatcac tagttctacc tcagggagtt tagttgccaa agcatcctgc
    28621 aatccttcac gcaacccttg attggccgct acacctccag ctacaatcag ttgcttgact
    28681 ggataatttt gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga
    28741 aagctagctg ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc
    28801 aaattgataa atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc
    28861 atcgctcgtg gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt
    28921 ccgcttgggt aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg
    28981 tcatctcttg tctccccaat gatttcatat gatccatctt cttgcatata gactaattct
    29041 gtatgtcctc cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg
    29101 gctgcataga tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa
    29161 gcaaaagcct tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa
    29221 gtgacagcga ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc
    29281 aagcataagg tgatttgttc tacgtgatgg cggctagcta cttcaggaac tacgccacca
    29341 aaacgtttat gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt
    29401 ctaacgaccg ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca
    29461 ttcatttcat cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat
    29521 tgcgataata tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc
    29581 ttgctgcttc attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt
    29641 ccgtaatcag taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa
    29701 cagcaatatt tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga
    29761 tctggttatc ttcaactaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa
    29821 actgcatttt cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc
    29881 attctgccaa gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat
    29941 tttctaaaag ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt
    30001 tgatcgttgt tgcttgaaaa cctaacttcc gataaagtct ttgtgcatct tgattactca
    30061 tacgcacttc taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt
    30121 caataagcag cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga
    30181 tatggcaatc taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa
    30241 agatacctag atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg
    30301 tccatggcaa ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt
    30361 ctgtagtcaa ttctcttagt acgtaacgac gatcgatttg ttcgattgtt ttttctgtat
    30421 aaatcatttt atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt
    30481 tcgtctccag gtgtgtgcgt ctccaaccat ttttcttctg cttctacccg ttttaaatag
    30541 cgaggaagaa aagcttgttt ctctgcaact ggttcagcta aagcacctaa ttgagccaca
    30601 gttacaccac ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt
    30661 tcctttattt catagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc
    30721 agcttttgta gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacaatt
    30781 tctaatctat tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca
    30841 aacatgggca caataatttt atccacaccg atgcaattag ccgcgatggt tttcagacta
    30901 gatactccta ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct
    30961 atacgaagcc ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt
    31021 ggacttattt gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg
    31081 ttgcgtttga tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc
    31141 gccaaagttt ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct
    31201 atgacattct cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag
    31261 gcaaaactag tacaatgata catgattatc tgctggcggg caaatttt
//
LOCUS       DACEDM010000034        30259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEDM010000034 DACEDM010000000
VERSION     DACEDM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.034"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            136..1455
                     /locus_tag="IWO50_001367"
     CDS             136..1455
                     /locus_tag="IWO50_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ0358498.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1896..3536
                     /locus_tag="IWO50_001368"
     CDS             1896..3536
                     /locus_tag="IWO50_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0358499.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3690..5012
                     /locus_tag="IWO50_001369"
     CDS             3690..5012
                     /locus_tag="IWO50_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0358500.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5074..5505)
                     /locus_tag="IWO50_001370"
     CDS             complement(5074..5505)
                     /locus_tag="IWO50_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0358501.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5569..6405)
                     /locus_tag="IWO50_001371"
     CDS             complement(5569..6405)
                     /locus_tag="IWO50_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0358502.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(6595..6897)
                     /locus_tag="IWO50_001372"
     CDS             complement(6595..6897)
                     /locus_tag="IWO50_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0358503.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(6923..7354)
                     /gene="ruvX"
                     /locus_tag="IWO50_001373"
     CDS             complement(6923..7354)
                     /gene="ruvX"
                     /locus_tag="IWO50_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0358504.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(7351..7617)
                     /locus_tag="IWO50_001374"
     CDS             complement(7351..7617)
                     /locus_tag="IWO50_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0358505.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7871..8596)
                     /locus_tag="IWO50_001375"
     CDS             complement(7871..8596)
                     /locus_tag="IWO50_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0358506.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8962..9032)
                     /locus_tag="IWO50_001376"
     tRNA            complement(8962..9032)
                     /locus_tag="IWO50_001376"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8998..9000),aa:Gly,seq:ccc)
     gene            9149..9901
                     /locus_tag="IWO50_001377"
     CDS             9149..9901
                     /locus_tag="IWO50_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0358507.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9917..10396
                     /locus_tag="IWO50_001378"
     CDS             9917..10396
                     /locus_tag="IWO50_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0358508.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10647..12227)
                     /locus_tag="IWO50_001379"
     CDS             complement(10647..12227)
                     /locus_tag="IWO50_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0358509.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(12220..12963)
                     /locus_tag="IWO50_001380"
     CDS             complement(12220..12963)
                     /locus_tag="IWO50_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358510.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(13139..13915)
                     /locus_tag="IWO50_001381"
     CDS             complement(13139..13915)
                     /locus_tag="IWO50_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0358511.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(14163..14999)
                     /locus_tag="IWO50_001382"
     CDS             complement(14163..14999)
                     /locus_tag="IWO50_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0358512.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(15010..15939)
                     /locus_tag="IWO50_001383"
     CDS             complement(15010..15939)
                     /locus_tag="IWO50_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0358513.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            16131..16484
                     /locus_tag="IWO50_001384"
     CDS             16131..16484
                     /locus_tag="IWO50_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0358514.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(16558..17214)
                     /locus_tag="IWO50_001385"
     CDS             complement(16558..17214)
                     /locus_tag="IWO50_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0358515.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            17440..19374
                     /locus_tag="IWO50_001386"
     CDS             17440..19374
                     /locus_tag="IWO50_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0358516.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(19460..20683)
                     /locus_tag="IWO50_001387"
     CDS             complement(19460..20683)
                     /locus_tag="IWO50_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0358517.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(20677..22575)
                     /locus_tag="IWO50_001388"
     CDS             complement(20677..22575)
                     /locus_tag="IWO50_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0358518.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(22569..23687)
                     /locus_tag="IWO50_001389"
     CDS             complement(22569..23687)
                     /locus_tag="IWO50_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0358519.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(23770..24603)
                     /locus_tag="IWO50_001390"
     CDS             complement(23770..24603)
                     /locus_tag="IWO50_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0358520.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(24596..25513)
                     /locus_tag="IWO50_001391"
     CDS             complement(24596..25513)
                     /locus_tag="IWO50_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0358521.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(25740..26864)
                     /locus_tag="IWO50_001392"
     CDS             complement(25740..26864)
                     /locus_tag="IWO50_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0358522.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            27047..27955
                     /locus_tag="IWO50_001393"
     CDS             27047..27955
                     /locus_tag="IWO50_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0358523.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            28096..29709
                     /locus_tag="IWO50_001394"
     CDS             28096..29709
                     /locus_tag="IWO50_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0358524.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9425 a         5948 c         5173 g         9713 t
ORIGIN      
        1 aattaaaacc atgtgaaact gcctcacggc agcttacaat tactttttca tcttcacccg
       61 ttaagtgatg aaaaaagaac ccctttctgc tatggttaaa gtgactaaac caaccaaaag
      121 aaaggagttc ttttcatgac tagcttacac aaaaaccaag taaaattcaa ttcaaatatc
      181 actatttctc atacaggtgg tcaattatcg agtgattcgg gtctcgtcct agtgaaagag
      241 ctgatgaaca tctttggttt ttctgaactg gctaagcaac acattcacat tgaagacgaa
      301 cgagcgtatt ttactcatga caacttatcc atacttgagc agttcattat gcaattaatt
      361 gctggttact cagctgattc tgcagctaat ctcctgagac aagatcctgt gtttaaagct
      421 gttttagata ggaaagaact cgcttctcaa tcttcacttt cccgattttt agatcgacta
      481 tctgaggaga atatccatga acttcaagct ttgaaccaag aacttatcga taaagcacgc
      541 ctcatccgta atgatacgga attaatcatt gatttagatt cgacccattc agatacattt
      601 ggtcaccaag aacaaacgaa ttataataca cactaccaaa cctatggtta ccatccattg
      661 gtagcttttg acggattgac tggtgacttc ttaaaagctg aactacgttc aggaaatcaa
      721 tatacatcaa aaggcgtaaa ggagtttctc acacctttat tagagcacta taatcactct
      781 ctaccaaaca ctgacatctt ggttcgtgga gacagcgggt tcgctacacc tggtgtgtat
      841 gattcgtgtg aatcaaaaaa gagtcattat gttattcgac tgaagaataa tcgtagacta
      901 ggtcaaatag ctgagaaatc agtactttat ggcgataatc aaaagtggga agaacgagaa
      961 gttcagtact tctcgaccac ttatcaagca caatcctggt cacaaagtcg tcgcgtgtgt
     1021 atacgctcaa cacgtgaagc gggcgaacta ctctttcgac atgaatttat cgtgacgaat
     1081 ctatcagaaa atgtttctcc tgataccatc ttttctatct atgccaaacg tggcacaatg
     1141 gagaacttca ttaaagaagc gaaagctggc ttttactttg acaaaacaga cagtcctcgc
     1201 tttttggaga atcatgtccg aatgatgctc agcttgatag cttacaactt agtcaacttc
     1261 ttaagaacga ttggctttga agaagtccaa aagggaatga ccattcattc tattcgattg
     1321 aagtttctga aagttgctgg gaaattagtc caaacgggta gacgagtcta tctcaaatta
     1381 tctagttatc atgtgtatca gaatgaattc tacagggtct ttgctcgcct gaggcgagcc
     1441 agtcaatgga tctaatccaa caatctaacg atttttttac tgggaagtta tccagggaga
     1501 agtatgctca aaatgcctta cacccaaaat ttattcctca atatttttga acagtgaatg
     1561 tttgaagtaa ctttttagtc aaaatcagtt gatagggata ttagtgatac atatttcaat
     1621 aaaatgtgcc aaagaattaa agctatgaat tattcaggat tctatttatc tatctaacat
     1681 atcctttatt cttctacttt tctttcttta gcatataaga gatacgcata gtaatcaagc
     1741 aaggattgtt gatataaaaa gaacaaatac cgctattttg atttcttttg actctattac
     1801 tcatataaat aaaatttatt atacctgctc tttttgacta tatcctcatc tgactcctat
     1861 acttcgtttg tattaaaact taggaggaat cattcatgct tacagggcta aacttattaa
     1921 ataatccttt tctaaataaa ggaacagctt ttacaaaaga agaacgggca aaatacggaa
     1981 tcactggtat gcttccaagt actgtccaaa cattggaaca acaatctgtc caagcttacg
     2041 gtcaatactt aagcaaacag accgatttgg aaaaacgtat cttcttaatg aatctattca
     2101 atacgaaccg gacattgttt tataaattga tgggcgaaca tttagttgaa ttcatgcctg
     2161 tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt ttcttgaaac
     2221 cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa tatcaaagca agtttgaaaa
     2281 acgcagcaga tggacgcgac attcgtttga tcgttgtgac agatgcagaa ggaatcttag
     2341 gtatgggtga ctggggtgtg aacggtgttg acatcgctat cggtaaattg atggtctaca
     2401 ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt atcaatcgac gctggtacta
     2461 ataacgaaga attattgaat aatccattat acttagggaa tcgtcatgct cgtgtagaag
     2521 gcgaaactta ctacgaattc attgatcaat tcgttcaatc agctactgaa ttattcccag
     2581 aattgttatt acattgggaa gattttggac gaggaaatgc tgcaacgatc ttagaaaaat
     2641 acgaagataa aatcactact ttcaatgatg atatccaagg cacaggaatc gttgtactag
     2701 ctggtgtatt aggtggattg aatatatcag gtgagcaatt gaaagatcaa acgatcttga
     2761 catttggtgc tggtactgct ggtgtaggta tcgcaaacat ccttttagat gaaatgatcc
     2821 gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta tcaagtagat aaacagggtt
     2881 tattgtttga agatacaaaa ggattgactc ctggtcaaat cccattcgct cgtaaacgct
     2941 cagaattcac taatgctgat gaattgacaa acttagaagc agttgtaaaa gcaatccatc
     3001 caacgatcat gatcggtact tctacccaac ctggagcatt cactgaagaa atcgtgaaag
     3061 aaatggcggc tcatacccca cgtccaatca tcatgccttt gtctaatcca acaaaattgg
     3121 cagaagcaaa agcaaaagat ttgattgaat ggacggacgg aaaagcttta gttggaacag
     3181 gtatccctgc tgctgatgtg gaatataacg gtgtaactta ccaaatcggt caagcaaata
     3241 atgcgttgat gtacccaggt ttagggcttg gtttgattgc ttcaacagct acacgagtaa
     3301 atgctgaaat catttctcaa gctagtcgag cattaggcgg aatcgttgat gtaacaaaac
     3361 caggagcagc tatcttgcct ccagtagcaa aaatcactga attctctcaa acgattgctg
     3421 aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg tgaagaaatc acggatatca
     3481 aagaagctgt tgaatctgct aaatgggttc ctgaatataa atctttagaa gattaagatt
     3541 gttatagtaa aagaacatac agaaactttg tatgacgtct aaaaagaaaa aagcgactgc
     3601 ggcaaaacag cagccagcca actactgctc ttttgccgct tctctttggc ttttttcata
     3661 cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa aacaacaacc gcaactgcag
     3721 ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat taccggcgta agtttgcccc
     3781 tttatttgat catggtcctt gtcctgatca ttgcaatggc tttaggaaaa ttaccgacga
     3841 atatgattgg cccaatcgct gttttggtca ttttcggtaa tttatttcat tttatcggaa
     3901 caaaaatccc tatagtcaaa agttatttag gtggtggctc cgtttttgcg atctttgcct
     3961 ctgccgcgat tgctacattt gggatcttac ctgagtatgt agtaaaatcc acagaaaact
     4021 ttgtcagcaa tatgggcttt ttagactttt atattgctgc cttgattact ggtagcatcc
     4081 ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg ttttattccc gttgcactta
     4141 tttcaatggt cgtctccttt tttgccgtag gactcgtagg tatgcttatc ggaaatggtt
     4201 ttgcaaattc cgttttatat atctcattgc ccgcaatggc tggtggtgtt ggagccggag
     4261 ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac ggacgcttct tctatcattt
     4321 cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc tatcattggt gcagcgttag
     4381 ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa tggtaaatta atggaaaaag
     4441 gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc cattccacaa ttaggtgtcg
     4501 gtttgatggt atcattgact ttcttcatct taggacaaat ctgtaactat tttgttccta
     4561 aagtccatgc ttacgcattc atgatcatta tcgttgttat ctgtaaaatc gcaaacctat
     4621 taccagaata ttacgaagat gcagctatca tgttcaacaa cttgattgtc aaaaacttga
     4681 cagcagctgt tcttgccggt atcggtattg cgttattgaa tctaaatgta ttggcttctg
     4741 ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt tatcgcgatt tcagtagttt
     4801 caggatttgt cggtcgtttg tttggtttat atccaatcga atcaactatt actgctggct
     4861 tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt cttatctgca gctaatcgga
     4921 tggaacttat tgcttttgct caaatgggca atcggcttgg tggtgccatc gttttgatca
     4981 tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa aatacctgat catcaagtat
     5041 cggcttgatg accaggtatt ttttgataaa aatttacagg attttcaaaa atcttttttg
     5101 cattttatcg gaatgataac cgatgcgttc ataaaatcgg tgtgcttcta ctctgtattc
     5161 tgcagaatta agtcttattg ctgatattcc tcgtcttttc gcttcttcta cgccagtcat
     5221 caaggccttt ccgattcctc ttttttcagc atgtttagac acagcaagtg ccataatatt
     5281 gaacatcgga tcgctgaaaa tcgattcata ggactccgct tgtacatagc cagctgcttt
     5341 tttagaagta ggatcttcat agaccaagaa aaaatttttt tctggctcct tcaacagttt
     5401 cttcatttgt ctcaccgtaa cggttaatgg tacatcatat cctaattgtt ccgcattgat
     5461 ttctcgtagt gcttctgcat ctcctaaacg catttcccgc aacatccaaa tcaccagcca
     5521 atttttattt ttttaaatga atttttttat cacagtatac tccttttttc acttaaaaaa
     5581 agacttttca gtgattttga cttgcttgaa tccagatcct tctaatgttt gcaagatgac
     5641 gaaagcattg taaagattga tcaaaagaac cacgatgaca atcgcttgaa aaataaaggt
     5701 cacttttttt ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc caataacagc
     5761 agcggggata acataaaaca acatagataa atcaaagcgc ataaagctac ttgttaaagc
     5821 aatcgttacc aattttgcca gttgagaaaa gaaaatcgta gatagagaat atagtgttgc
     5881 ttcttttatt ggcaatgcaa acatcagcat caacaaagaa acattgactg gtccaccacc
     5941 aatacctaaa aaactagcta aaaagcccag aaccccccca catataaggt accaagtcca
     6001 tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt
     6061 caataccgtc aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa ataactgtaa
     6121 aaaaacttcg aatacaatgt tgccgactac tcctccaaca acagctccac tagataccca
     6181 accgacaatc tgccaattca gtgatttccc gcttgatact tgtcgcacag tcgaaacaat
     6241 ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc
     6301 gataagatca agaatcggtt tgatcaacac gcctccaccc atcccggaaa tagcgccaac
     6361 tgtgttcgcc agaacgatca ccacaaaata aatcagacca atcatcttgt atttcctttc
     6421 tcttgtttat atcttttagc gattgttact cttttctttt attatacagt aaaagaaaca
     6481 aacgaggctt cgaaagcttt tctacttttt aaggaaattt agtgctaaac gaaaaaaccg
     6541 ggctctactt ttcagagagt ccagcttttc ttacacctta tcgattcttt tgttctattc
     6601 ttcttccgcc atgaatgtat tgaatacttc ttcgatcata tcccattctg cgtctgtttc
     6661 gatttgttct aactcgcctt ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa
     6721 ttctacatct tcatcttctg aaacaccagc tggatataaa agaacatagt tacgtccaaa
     6781 ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat gtttcatttc cttggtcatc
     6841 caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa tggttatgtt ctgtcataag
     6901 attacctctt ttctttttat tattaaaata attgcttacc tgagccgtct aaataatttt
     6961 gtaaaatcat cacagctgct aatttatcga tgactttttt tcgtttcgct cttgaagtat
     7021 ttgcctgctc tactagcata cgttctgcct gaacagtggt tagtcgttca tcttgataat
     7081 caaccggcaa ggagaatagt tctttgatct tctctccata tgacatggaa gcttctgccc
     7141 ttggtccgat ggaattgttc atattcttag gtaatccaac tacaaaacgg tctacttcgt
     7201 actgcgccac taattccttc aaccgatcaa aaccaaactc ttcttctgcc tcattgattc
     7261 gaatgatttc tactccttga gctgtccagc caaagggatc gctcacagca acgcctactg
     7321 tcttagaacc gacatccaag cccattactc tcataaattc actccatgat ttgagaggta
     7381 gtctttgaca attacttcca tgatttcatc gcgttcgtga cggcgaatca aatttcgtgc
     7441 atcacgataa cgaggaatgt aggccgggtc tccagaaagc aagtagccga caatctgatt
     7501 gattggattg tagccttttt cttccaaagc ccgataaaca atcgctaatg tttcgctcac
     7561 ttcttttttg cgactatcgt caaaaccaaa acgtacggtt tcatctgtaa aacccatgat
     7621 tctacacctc tttcccgcaa ggtaattcca gcttaccttc tacaccctta ttttactcga
     7681 agaaagggaa aactacaaac aaatattcca aacttctgaa taaattaaga aacacgattt
     7741 gtctgatttt tccttttttc tgtggctttt tcaaaagccc tctttccata aagtatacgc
     7801 tatatttctt tgaaagtatg tttttacaca attttttcat aaagttcata aaaaattcga
     7861 attgctgcta ctaatttttc gttttcgttg cttgccctgt ctcataattg atggtgtagc
     7921 catcttgaac attgtagata aagacattga attcaatttt tttgtcttca atgctttctg
     7981 cttccaactg cacacctctt gctaccagtt catttccctc gaaatgagga gtgacacgat
     8041 agcgtacatg atgatttgtt tccttgatat atgccgcaat atcattttcg tgggcaagca
     8101 tgtacggagc attcaacgaa cgtgtccctg tcattaagtt tttaatgtta ttattttgac
     8161 cagtcaactg gaaaccaatc aaatggctgc ggttgtataa ccattgtccg tcacttaatt
     8221 tcttttgttt ccaacctgtc ggatcaatat acaaaggttc tctcttttca gtaggaaaca
     8281 agtcttttcc caacatcgca ttggcttgtc ctactctgtt caatttgtct atatttgaat
     8341 agctttccca tgtccctttg gaaagactaa gatcctcttt tgtaaactcc ggctgattcc
     8401 cattgatttc tatttcttga tgttttccat catattctgg cacatccgct acttgagaag
     8461 cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa gatatctgaa tctttttgtg
     8521 tggtggaaat accgagtgcc cctgcaatca cagcagcgac gatccccacg atcgccttgg
     8581 ttgttttttt gctcatttct ttactccttt actaacagtt ccctaaatct taacatatgt
     8641 tcccctataa ataaatactg gaaagaaata gaaacagtaa aaaaataaaa ccagttttta
     8701 gacggcttaa gaatacaaaa aaagagacgg attcatcgtc tctactcaat cacaaaacac
     8761 tatttacaag gtatttatta tcattcaaaa aatttttctt aatactcaaa actaaacggc
     8821 ttctgtacga cctcttggta aacagtgttc aaaagcttgc atctgcggta ataagtccaa
     8881 ccaagtcaaa aacataagaa gcttttttgc ttgactattc ttattacttg atgcagggca
     8941 gctttttcac agcctctttt aagcgggtga cgggaatcga acccgcgaca tcagcttggg
     9001 aagctgggat tttaccacta aactacaccc gcgatacaaa aacagtatac gcctctttca
     9061 ctccttcctc aagttttttt attctttacg cttccgatat gttttgtagt aaaataaaac
     9121 taatcaatca attattggag aagatgctat gacgggaaaa gaattagatc tgttgctgga
     9181 cacttgttta ctcgctggga aaatcatgat ggaaagcaat gcggagatgt atcgggtaga
     9241 agatacgatg agtcgaattg ctttagcttc tggaaatttc cgtttggtca gctatgtcac
     9301 tcaaaccggt ctctttattg gtcttgatcg tacctcgacc atccgaatgg aacaaattac
     9361 caaccgttcg attaatttag aaaaagtagt aaaagtaaat gatctttcac gtaaatatgt
     9421 tgcttcagag ctaacattag aagaacttta ctgcgaactg caagcaatcg accgagaccg
     9481 ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat
     9541 ggtgttattt ggcggaaaga tgaccgattt acctgtgact ttagtcattg gaggattggg
     9601 atacgcagct tatctatata gtttgaaatt ttttagaatc aaatttttat cggaattttt
     9661 atcttcttta ttgattggca tagccgctat ctacagcgta cgttttggct taggaagcaa
     9721 tcaggatcta atcattatcg gatgtgtgat gcctttagtt cctggagttc aaattacgaa
     9781 cgctatccgt gatttgctag ctggacatta tgtctcagga gtttctcgcg gaacagaagc
     9841 gatgatgact gctacgatga tcggttttgc gattgctttt attttccagc ttttttatta
     9901 ggaaaggaac acaagtatgg ggacgatatt tattcagttt tcttttagtt ttttagcgac
     9961 tgcggcattt gccgttatta caaatgtacc taggcgttca ttgatttgtt gtggtctttc
    10021 tggtgctttt ggctggatga tctactggat ctgtgttcaa ataggcggat ctgctgcttt
    10081 tggttcattg cttggttctt taggagtagc attcatcagc gatcttttct ctaaacgttt
    10141 gaaaatgccc gtaacgattt tcaatattcc cgggatggtt cctctagtcc ctggtggttt
    10201 agcttatcaa gcggttcgaa atctcgttac tgaatcctat cgagaagcgg ctcattatgc
    10261 ggtccaagca attatgattg ctggagcaat cgctttaggt cttgtattat cagaagtctt
    10321 taaccataac atccgtaatt tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa
    10381 acggcttttt aaataaacga agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct
    10441 tgagcgatga caaaatatct cacaaacatt gcgacaagta accgcaggag caataagaag
    10501 taattatgag tattaaaaaa agttttgtaa aaatagcttc tcacattttt acataaaatt
    10561 gacttaatac tgagaaactg acttttgaac accctctatc tgtaaaaaag gggctgcgac
    10621 atgatttttg tcacggcccc tcttctttat tcaaattttt tccaaaacgt tcgttgataa
    10681 taaacgatcc atagagcgga aagtattaga actaaaccga tcacaggaat attcactaac
    10741 caaaatggat aaaacaaagc caacattccg tataagacga taagaataat actgatgatc
    10801 aatgaaccca tcatagttgc gactaaaccc aaacttcctg aacctctgtt aaataattgg
    10861 ttaatattgg tccagttcag cagcagcaac cgataatctc tagcgaaata acgtagtgac
    10921 aataagtagc agccgaataa agaaccaaat agcaagctag ggataaaaaa caaaggcagc
    10981 tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta aaaaactctg caatatccat
    11041 ccgaatctga acttttcttt taaatattgg ttcatagaga ttggcaaaga acggataaac
    11101 agaaaatttt cttgatccaa agaaataaga ttgctgataa aagaagtctg attaactgtt
    11161 aagaaagcca atgcgatccc taccaggaaa acaacaccga ttaacttatt atctaaatca
    11221 gttaatttta tttcgcctcc tagggcaaaa gcaagaataa aaatgattgg agttaacaag
    11281 gaagaagaaa gaacttgcat aatcaaatta ggttctttta ctaattgagc attgtaacta
    11341 aataaaagct gatgcacatt ttggttcttt ttatgggttc tttttatttt gaactcactg
    11401 tttgaatcag tcagttgctc tgtaagcttt ggtactatca gctgttttac agcaaggaaa
    11461 aatcccagag ccgtgatagc tagtattccc caacccaata aaccttgttg agaaagtggc
    11521 tgactggcag catcatagaa tggcaataat atcgaaatcg gatgccgatc tgttaactca
    11581 ttttcaatag tccccgtttg atgattcatc cacatgattc cagcaaatgc gatcagcatc
    11641 gagctgccaa gcaagatact agaaactgtt tttctatgtt ttttaaacac tgctgttcga
    11701 gccaaaccaa aaacaatcaa actagccaga caaaaaagaa taatcaaaaa tagagaaaac
    11761 aaaaggattg agataatgat agtaagcaaa atccctagat cagctcgcca tccggttaaa
    11821 ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc
    11881 tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca aaaatacttg caggtcattt
    11941 ccttcaaaaa agatattata aatgactgat atactttgag ttaaacctaa cataccaaaa
    12001 agtgccacgt aataagtaaa gaaacctggc aatttaacaa agttcatagc aaacatagca
    12061 aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat tttgagacaa aagatagata
    12121 gttaatcgct ttcccttttt tcctttctct cttgctttat tcgtgagctg tggattaaca
    12181 tataaaagat taactcgaat taattctaat aattgtcgct tatacatacg gtccatctcc
    12241 tgtcgcttgc cttccagcca ttttcaaata aatcttttct agcgtttcat caggtgagcc
    12301 attcaataat tcactaactg ccccctgata aatcaattca cctttcttca aaatagccag
    12361 tttatcgcat aactgttgtg cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc
    12421 ttttgcggca tgagccttca tcatttcttt caaatcaaat gctgcttgag gatcaagtcc
    12481 ttgcaaaggt tcatccaata cccaaatatc tggatctggc agcaacgtgc cgatcaaaat
    12541 cgttttttga cgcattccat gtgaaaaact tgcgattgtt tcattttgat gactatacat
    12601 atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt ttttgtgtgt ctaattcata
    12661 ggcagctgaa atcaagtccc aatattctcc agccgtcaat tgtaaaaaaa tatctgggga
    12721 atcaggcaca taaccgattt tttgtttgat actcaatcga ttttccgtca atttcattcc
    12781 atctactaaa atcgttcccg atgatggttc aataatacta actaaacttt taatcgtagt
    12841 tgattttccg gcaccattat gtcctaaaaa tccaaaaatt tccccttgtc ttatcgagag
    12901 agacaagtct tttaatgctt gttttttccc gtaactttta cttacattga tgaattcaat
    12961 catttctgta ctccttttgt ttgttattac tttttattgt acccttcgtt attggtggag
    13021 taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac gactaggctg ctacttgggt
    13081 tgttctcttc tacaggaaaa cactcttcta acaggtaaga tatgagtctt tgttttcttt
    13141 aaaatgattc attttctatc ttttcttgca taaaattctt tagtttatca atacttctct
    13201 ttcccatctc tagttgttct ccatttgcaa atgttactat ttcgtttact ctgctaatag
    13261 atgtaatgtt ttcagtatta atgatataag cttttaaatc tttacataaa tactcatatt
    13321 cgagtgattt tttatatttt ttaattgtgc cgtttacgag aatctcttca tctttcaata
    13381 ctaggcctat tttattttta taacctggca tgctcattag ataaaggatt tcatcgtaat
    13441 ggatatagtg atcgctatta ccataacgga cagttgtcca cctttttttc gtagcatgtt
    13501 gaacaggata gttttctttt tctttcaata agtaactttg gatgcttttt ttattttttt
    13561 cataagtgaa ttcttttatc aaataaccac taggcgtaat ctttcgatta ataacagtta
    13621 ttgctttatt cggatgagac gttagataaa caattttaca cttttcattc ttttctctaa
    13681 gcatttcgcc tagttcgata ccgctataat aggcgttcaa atcgatgtct aggaaaaaaa
    13741 tatctgagga atagatttca ttatttttaa tgaattcaac aaattgtata acgttcaatc
    13801 cctgaacgat ctcaaatttc caaggactta ataaaattct ggattcttcc aaaatttcta
    13861 ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa tatacgcgtt accataaaaa
    13921 gtacctccac tcgatatttt actatcaaaa tcatttcata caacttttca acatacatgt
    13981 taggaataaa ttgtaattct cttcacaatc ttttccaaca cctttgaaac cgatatcaaa
    14041 tactttcagg atgctgcttc taaaataaat atcaaacaaa tgaatacatc atgcaaaaaa
    14101 agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc tatgtttatt tattttgttc
    14161 ttttaaaaag aacgatacac atactgattt ttcggagcag agactttctc agcgtttgta
    14221 acaactacag atgggatttc acgcttgaat ggttgataat agtctgattg gatggttcct
    14281 tctattttat accattcatc atttttaacc gttactcctt ccggcatatg cgtcaacaaa
    14341 ccaaagactc ctgaatctgc cacacaatgg ataatgccga atcggaacaa gaagatatct
    14401 gtattgctat cgttcggtgt ttggtaaaca aatccttcat agctgatttt tttgccaatg
    14461 aattcgctag ggtaattata aattagctcc ataacttcta aatagttttc atctgtgatc
    14521 ttgatagttt gcttatcccc atacttttct ttcaactcat tcatttggtc taagtagtct
    14581 gatttgttga aataaatact ggtatccggc tttaagtatt gtgtctgcat ctccggatcc
    14641 cctactgatt ctttacttac tgggaaatta aatcctttgg cttctacgat cgtagtgtcg
    14701 agactaactg ttggaaacaa cgtccccacg attactggca aagctagcag tacataagcg
    14761 attccacgtt gataaggttt tgacaaccca tggtcatgat cgtgctcgtg tgccttttct
    14821 tctgcttttc cgtttttgac ccacaaatac agctgaacta aagccaaaag agcagataaa
    14881 atcattgaaa gaatagctaa gtagcgataa tggacattga tatattgatc gagtttgcca
    14941 gaaacttgca agtacatcat caattcgaaa tacccagata aaatcaaaaa ccgcagcata
    15001 ttttccctcc taaataaata atgtgaatcc aataacgcta agggcaatca gtccgatcag
    15061 cgataaaata aagcgtcctg taaaatatcg tttcatcatc agtagatttt tgatatcgac
    15121 cattgggcca aagaccaaaa aggcagctac aggtgctgtg ccgaacagac ttaacaaaga
    15181 agatcctata aacgcatctg cttctgagca aagagacatc gtaaaggcca ataacagcat
    15241 gatgataatg gctaaaatct ttgttgagcc gagcgttagc ataattctag ttggtaaata
    15301 agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa taacgacctg tatcaaaaaa
    15361 ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg atcagctgat tttttggttc
    15421 attcttctca ctttgttctt cttcatggac atgatgatgg tgattttctg ctactttaga
    15481 cgtgattttt tctttcaata cagcgtcttt ttgaaaatag gcaagccaga caccaatcac
    15541 aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc atctgccaag aattgccaaa
    15601 tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc ggagcagtca gcataaaagc
    15661 aaacgctgtg tgaactggta cgcctttttt aacaaattga tgaacgatcg gtacgatgcc
    15721 gcattcacat gaaggaaaga aaaatcctaa gatcgaacca gtaaaaatag agaagatacg
    15781 attttcagga agccatctct tcacacgttc tggggtcaaa aatacttgta aggcgcctga
    15841 gataagacag cctaataata cgaaaggtaa tgcttcaata acgatcgata agaaaatcgt
    15901 actcatctgc aaaaccgaat ttggaataaa agaaaacatg agtttcctcc tttttatttc
    15961 tagttcattc gctttgcaaa tatacgcttt tattcaaaaa aaacaagttg ctctgtaaat
    16021 ttttacacaa ctcttatttt ttaccaaagc acttcaattg actttttctg ttattcccat
    16081 taaattaagg tctcatacat atcacttcta tcataaaagg gagaataaaa atgaaacaaa
    16141 ccactaactt gactgaagtt caagacatac tgcaacagtc atccgttgcc gtcatttatc
    16201 tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc acgactagag gaacttctca
    16261 cacattatga tattccggca cttcatttgg atgcatttga aacaccagaa gtagctagta
    16321 aatttgaagt gctgactgcg ccagttgtcc tgatttttca tcaaggaaaa gaagtctttc
    16381 ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct actagatgaa ctgacagcag
    16441 aagatgattc cctttcttat gaagaaatct tccgaacaaa ataatcgttg tccaaacaga
    16501 aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt tcaatcgctt tttattttta
    16561 ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc aagaaataga tcaaggtttg
    16621 caattcattc gtcaaatcaa cattttggat caatacatct gacggagcat tcaatcgagc
    16681 agcggtaaag ttcaagattc ctttgattcc tgcttccgtt aactgattga ctacttcttg
    16741 tgctctttca gcgggtatcg ttaaaattgc tacttcgatt tgttggatct tgatttgttc
    16801 tgacatatct tccattggat aaacggggat accgtctacg atgcgcccaa cgatttcctc
    16861 attcacatca aaagcacaac tgatacgtat gctattactt tgatggaatt tgtattttag
    16921 taaagcactt cccaagttcc ctacaccgat cagcgcaaca tttgtcaatt gatcgtcgtt
    16981 taatgtcttt gcaaaaaagt gcataagatc ttctacatca taaccatacc cacgttttcc
    17041 taattcgcca aaataagaaa aatctcgacg gatcgttgca ctatctactt gaacggcttc
    17101 acttaattca gttgaagaca cttttttctt tccagcatta tttaaaatcc ttaaatagcg
    17161 ataatacaaa ggcagtcttt tagctgtcgc ctttggaata atatgatctt tcacgaaatg
    17221 acctccacgt attgtctaat tacatctttg atttctccat catagcagta tttataggaa
    17281 aaaatcaatt ccattgctca gatattgtga aattttattc tttattcatt tatttatcat
    17341 ttctgcaaat ctcgcagtat aatggaattt atggtagact actaattgaa aaatagtttt
    17401 ttatttgagg agaattcaca aagtagaaaa gaggattcca tgatattatt acaagccaat
    17461 cagattgctc gccattttgg tgcagaagtc ttatttgaaa acatacattt agaaatcagt
    17521 acaggcgctc gtgtcgcttt agttggcaga aatggcgctg gaaaatcgac tttattaaag
    17581 atcattgctg gaattgaagc acctgatgaa ggaaagatcg ctaaaaataa aacagcgact
    17641 ctcggatacc tcgctcagga cacaggactt gattccaatg aaacagtttg gaacgagatg
    17701 ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga tgcgcgagct agagattttc
    17761 attagcgaag gtacgccaga cacacctgct tatagttctt atttaaaaga atacgaccgg
    17821 ctgcaacacg atttttcaga tcgaaacggc tatggttatg aaaatgaaat tcgttccgta
    17881 ttgcatgggt ttaaattcga ttcttcattt tatgatcaga aaatcgatac tttatcaggc
    17941 ggacagcgca cacgtttagc tttagctcgc atgcttttgc aacaccctga cattttgatt
    18001 ctagacgaac caacgaacca tttagatatc gagacacttt cctggcttga gaattatctg
    18061 caaagctata ctggtgcatt attgattgtt tcccatgacc gttatttttt agataaagta
    18121 gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc attacaaagg taactattcg
    18181 aaatatctag atttgaaagc agaacagtta gcaagtgaat ggaaggctta tgaaaagcag
    18241 caaacagaaa tccataaatt agaagacttc gttgctcgga accttgttcg cgcctctacc
    18301 acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa tggaccggct ggatcgtcct
    18361 caaggggatg aaaaacaagc aaactttcta ttcagtatag aaaagccttc tggaaatgta
    18421 gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg tcaccttatc tgaaccaatc
    18481 aatctagata tccgtcgtca agaagcgatt gctctagtag gtccgaatgg tatcgggaaa
    18541 tcgacgttgc tgaaatcaat cattcaccag atccctttta tccgtggaaa agaaacgcta
    18601 gggacaaatg tcaccatcgg ctattacgat caaggtcaag ctgatctgca tacaaacaag
    18661 acgatcctag cagaattgtg ggatgagcat cctaccactc cagaaaaaga gatccgaagt
    18721 gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa aaccaatccc tctgctaagt
    18781 ggcggtgaaa aagcacgggt tgctttagcc aagttagcga tgaacaaaga aaattttttg
    18841 attttggatg aaccaacaaa ccatttagat attgacaaca aagaagtatt agaaaatgca
    18901 ttgatcgatt atgacggcac actgcttttt gtttcccatg accgatattt catcaatcgg
    18961 atcgcgacaa aagtcgtcga actctctgaa aatggcagca aagtctatct aggagactat
    19021 gattattatt tagagaaaaa gcaggaagaa gaagaaatcg ccgaattgtt ggcacaagat
    19081 gagccagaat ctgctcctgc tcccacgaac aaatctgatt attaccaaag caaagaacag
    19141 caaaagctta tccgtagcct tcaacgaaaa attactcagg ttgaagaaga aatggcgcgg
    19201 atcgatgaat ggatcgatca gttggaacaa gaaatggtcg cgccagaaaa cctggaagat
    19261 cacgtaaaac tcaatgagct gaaccaagat ttagaagctg ctagacaaga tcaagaaaca
    19321 aaattgacgg aatgggaaga actaagcttg gaattagaag aatatgaaaa ttaacgaaaa
    19381 aaccaaaaca gaaaccattc atgctgtttt ggtttttctt tttttatttt atcaaacaaa
    19441 aattccatat ttaccttttt caccaataag aagaccaatt actagtcatt ctcactaaag
    19501 cttctaaata atagtaatca ccccaaatac aaggtgtatt taccccatta tttttgggct
    19561 tattataaac agactgtgtt aaaaagccat cttcttcttc cgttgtagca tatctttctt
    19621 tcaattgtcg cataattttc caaacagcag actggtataa gttcttttct gttttgtcta
    19681 atagacctga ttctattaat tctaataatc cacaaaccat gatcaccata ctagatgtat
    19741 ctttttcttc tctgctagaa tcatcaaaga tcaaatccca ataacaaatg gtatcttctg
    19801 gcaaccgatt cataaaataa ttggctaata ttttgcttaa atcaaaatat ttttgttctt
    19861 tcaaataacg ataacttaat gcaaaaccgt aaatccccca tgcttggcct ctagcccaac
    19921 aggaattatc actataacct tgatgcgtac ttccatatct tggttttcca tctttgacat
    19981 ccatataaaa tgtatgataa gttgaaccat cttctcttac taaataatct gctgcatttt
    20041 gaatatgagc ctgagcagct tttttatatt tttgatttcc agtttcttca ctcgcccaat
    20101 acaataatgg aagattcatg caacaatcca tgatcattct accttgttgt gaaggatcac
    20161 taggatctcc ccaagcttgt ataactcctg ccttctccca aaaacgctga agtaatgttt
    20221 ctgcagcatc aattgaagct tccttcattt ttcgatttcc cgttatttta taatctgcta
    20281 cggttgataa agaatacaaa aaaccaagat cgtgatgatt agtgataatt tgacagtcaa
    20341 tcctgtggat aaaatctata tctgcttttt cagctgtttg ttgaaataaa ggatcacccg
    20401 tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc ttctgtccaa ccaatattgt
    20461 ctcttaatgg ataacgtcct tttataggac actcgcttgg gaattgatac tgactgttac
    20521 tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg ctcgatctca ttattccatt
    20581 ctttttccat taattcttta taacattcat ttatccattt ttctggatgt attatctctt
    20641 caaacatcac tttatcaagc atttccccac cagcctttag cataatttta gtcttttttt
    20701 attgttttta ttttggatag ttagcccggt tgtcccatca aaatcaattt tgggacgatt
    20761 gttccatact tctaaactat aatcccgatc ttgatgagca accactcctg ttccgagcca
    20821 atgggttcct tttccaaatg taccctctaa tccaggaata aaagtgtttt cccatgtcat
    20881 caagtttgta ttgggcaaac ccataatagc tgtggatttt cgtttaatag tatcgtcact
    20941 taaacagata atacctgaaa agccagcttc actcacccta tagttttcct tgtcttcttc
    21001 tatgattatt ccttctttct caggagaaca ttcaatagca aaccctgtct catatagaac
    21061 atattctgta tctaattcta ctttgtgtac gcgaatctgc cagccttcaa aaggaataag
    21121 ccaagttctg atttttgcct gtaaagtagg ttcccatgta gaggcaagta cctctttctt
    21181 acatacaatg tctcgtacat tatttcgaac gatgaactgt ccataggttt tgatgctctc
    21241 attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga atccctagca ttgaatccat
    21301 tgccatttct gctttcaatt gattcgctcg cggtatcgaa aaaccaaatt gactagaata
    21361 agcaaatttt aaatattttt cattggtcaa tgtatggaaa tttggcccta attgtcccgc
    21421 attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc aaattggctt ctgaaagaag
    21481 tttagttcct tcacgaatag gcataggcat ttctttagcc gtccaatact catggtcttc
    21541 aggtagcgcc aacaataaaa aaatcttatt cagccataat ggagataacg tactattata
    21601 gggttcagtc aatgtcaatt gagggtaggt ataaccaata ttcaggatac ctttttcatc
    21661 aaatatttct ttttgcatcc accatcttat attccgattg actaacccct tgatttcacc
    21721 taatttaaaa ggcaaaagat cggcccacaa catagctgac caaaagcttg aaactgcaaa
    21781 acgataaatc atgcttcgac caaaaggcac atttgcacca tcttccgcaa aaaaatgaat
    21841 atagtctcta gaaaaatcaa tagctcggtt cttatatatc tggctaatgg gcttttctgg
    21901 ttctagtacg ctataaatca aaccataata atgaaatgca aaaggattat aataatcttg
    21961 gcgacctctt gaactgtctc gataccagcc attttgaaga taacaatttt ctatattatc
    22021 aaatgcttct ttcagtgctg attctttaac tggataacca aatttttgga taattgttcc
    22081 gacaattatc ttaaaaaatt gccaattacc atctgcacat tcatattcaa aaatttgcga
    22141 gaaccaatga ataatctggc ttttttctcc tgtatctaat tttttccata ataattgttc
    22201 atgatgatat aacgctaata acattgcggg catttctacc atacgctggt ctctttcatg
    22261 tatttctccc caataatcgg gatgggaagg atctgttcca gcttttattc ctgatagaat
    22321 taatttcaac catcgttcat ctaattggtc aattgcgggc cccgctcccc ataaaacccg
    22381 agagaatcct tccattcctg caattttatt tccatatcct accccttgat gtatataatc
    22441 aatttgtgcg ccacttgctc gataaaacgg ctggagtggt tgatttatct ttttaaataa
    22501 gtcaaagtaa tcctgtctgg tccttaatag tggtactgga tgtttcattg tattttctcc
    22561 tctttttact accaaatagc taattccgac tgttctaatc gaagtaagcc ttcaataaaa
    22621 aaataatcag aatagatcag tggcacatta taagtcagtt ctctatggta tgcttctgct
    22681 ccgcctccaa tgatcccatc ttcatccata ttccaatttg catagtgctt ttctgtcttt
    22741 tggagtatat tgatagcagc ttcatgatac atttttgatc gtacagagga aacatatcct
    22801 gctaattcca gaagaccgca tgcagcgcat actccagcac tggtatcaat attcgaatct
    22861 tttacagcaa aatcaatgag aggaatataa ccagtttttt cgattttctg aataaaattt
    22921 actgctatct tttctgctat agctagatag tacggatttt ttgtgtaacg ataacttaac
    22981 gcgaatccat ataaagccca tgcatttcct ctgctccaac aagaattctc attgtatcct
    23041 tgcccgccaa gtaaccgaat atatttccct gattctgcat caaattctgc gatatgcccg
    23101 gttgatccat ctttcctcac tagatactta gctgttgtat ctgcatgctg ccgagcaatt
    23161 ttagagaacc tcggatcacc agtttccctc gaagcccaat acagtaatgg taaattcatc
    23221 atactatcaa taatcatcca accaggttta tggtcattcc atgctactaa atagttccct
    23281 gaaatattat agcgtccagc aagtaccgta gctgcatgta accctagttg tcgactatgt
    23341 ttgttttgag actgttgata atgtgcaata gccgatggta agaataaaaa acctacatca
    23401 tgatgaacgt ctgaaaaatg gtacagggca gattctaata attgttcttg ttgtattgct
    23461 gcctctttga acttttctcg ttttgtcttg tgaaagagct gccataaaat accactccaa
    23521 aaaccattgg tccaccagtc gattcctctg tcctttatta cgtctgtata ttttccgttg
    23581 taagaaatat atggaatttt attccccatt ctgtctaatt ccgcatctat cttttcagaa
    23641 gctacattca aaatttttct tgaccattcc ttcgtctgtt cattcatgga tatcccgcca
    23701 ttcttttaat ttttatgaat gtggagttgc tcctagaacg aactagatgg ttaacattcg
    23761 ttattttttt tacttatttg cagctccaat gtcattctcc ttaaatttag actgcgccca
    23821 cacataaatc aagaccattg ggatcactga agttgctaaa attagcataa ttgcgccata
    23881 attaattggc tcacctgtac tagaaaccgt catatttttg ataattaagg gaaccgtata
    23941 tttatcttta tctgaaagca tgactaatgg aaatagatat tgattccaac ttcccataaa
    24001 gataagtaaa ccactagtaa taatcgtagg tgataaaatc ggtacagcga ttttcgtaaa
    24061 aatcacccac tcattggcac catcgattct ggcagcctcc atcagtgctg taggaaattg
    24121 actgctatat tgtcgcatca taaacactga agttgtactg gcaatcgaag ggatgatcac
    24181 acctgcataa gtattcgata atcctaaacg actcataatg taaaataaag gaatcaatgt
    24241 ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg aacaacagtt tattccccat
    24301 aaaacgatac tttgctaatg cataacccgc cattgtagaa ctggctgtag ctgaaactgt
    24361 actgattaca gcaataacaa tcgaattcca aataatcaag ggataattat atctcgccat
    24421 gatggcttgc cagtttttta atagcatatc cccaaacttg aaattgattc cgccacttaa
    24481 aatttcttga ttactttgcg ttgaagctac aatcatatat aaatatggca tcaaaaatat
    24541 taaggcacca aacacaataa tgacgtacgt aaaaattctg ctttgtatct tttgtttagt
    24601 catttttatc acctccgaat ttaaattgaa tagccgataa tataacagat acaaacacca
    24661 tcgtccaggc aatagcagag gcttttccaa aatcaaccat ttcaaaactt gttttatata
    24721 aattgactcc tacgaccaaa gctgaatttt ctggtccatt ggaagtccca aagaatatat
    24781 ttgggatatt gaaattaccc aagccattga tagtggctaa aactgtggcc attaaaatga
    24841 ttggctttag catggggatc gttaataaaa agaattgccg aaatttcgat gctccatcta
    24901 tttcagcact ttcgtatatc tctttagaaa tcccttgaag accagctaaa aataaaatag
    24961 tgttatatcc tacactaagc caaatcatcg tgatgatcat tgatattcgt atccaaaaag
    25021 gatttgttaa ccactgtaca ccgttaattc ctagcgcatt caaaaatcca ttgaataacc
    25081 cagtcggatt gaaaaaagtc atgaatacgg aagctacagc caccgttgat gtaatggttg
    25141 gaatataata gacagttcta aaaaatcctt ttccttttaa ctgtgctgaa ttcaataaaa
    25201 cagcaaagta aatagctaat agcatcgtta ttggaattga tcctgccatg ataatcaaaa
    25261 cattcgtgta agatttaata aacaatggat ctcggaagat agctagataa ttgtcaaatc
    25321 caacaaattc aaatgagtag ccaattttca taaaactaaa agcaaacgag ataatgatgg
    25381 gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt gaccaatcca taagcacttt
    25441 ttttatgaga cgtatctgtt gcttttttct tgctgttctt tattgtcttg cttggtaacg
    25501 aagattgcat catcgttaag tcctcctaat attttttaat caccagttaa ttttacgccg
    25561 tacttatgag caaaattttc tgcttctttt agatatgaat tttcaaaatc acctgtttgc
    25621 caatagttat atgcagcggt agtcaaataa gacgtagcat ctgaataagt ggctggtaaa
    25681 acagtatttg atgtattttc attgatttta ttcaatagtt catagtattt ttgtcctcct
    25741 tagtagtcaa actttttctc agctgcttct gtttgatagg ccaatgagtt agatgtagcc
    25801 acgcccagct ccagtgcgtt ggctaagcat tcttcatctg tcagcataaa tgtaagaaat
    25861 tgttgtgcca cttgtggatt tttggcatct ttagggacat aaaaacttga tcctcctgat
    25921 actgcacctt ttcctggatt ttcttttgaa aaaggaatca gtggagccat gcgccatttg
    25981 tcagatgcat caggaaaatt caaagacata ttcgtagcat accagccgcc agcaacaaac
    26041 attgcagatt cttgaaatgc tttctcagca tcttgtgcac cgtagaaatg tacaatatct
    26101 gagtcgatca tttctttgat taactttgca gcatgtttcg cttctttagt attcagacgg
    26161 atatttcctt cttcatcaag caaagattga ttctgctggg ctagcatgtt gataaacaat
    26221 tcttggtccc cagattctgg caaggcaatt ccaaaactac cagttttttc ttttaatgct
    26281 tttgccacct ggatatattc ttcccaagaa ctgatttctt caaaatctac acctatttca
    26341 gcaaattttt ttggttgata ataacaaagt gctggggtaa aatcactagc ccaaggaata
    26401 atcgtttcat tactcgtttg ttgacgcaaa atattcaatt tttgatcata aaactgatcg
    26461 ccatatttgt cataaaatcc atattcatca gcactataaa aagcatcttg aaatttttcc
    26521 agcacacctg ctgctgaagt gtctgccatg gagataatat ccggcatatc ttcgtgtgtt
    26581 accaattttg gggtgatcat ttgtactgtt gctgttcccg aagctacatt cacaattttt
    26641 aagtcaatat ttgctccagt tttgcggtta tagatttttg cagcattttc aacatagttt
    26701 gtcagtttgc cgtttgcgac atatacttct aatgtagtgg ctttttttat cattggatct
    26761 gcgatgtttg ctgttacttc ttcgttagtt tttccacatg cacttaagct gaataatagg
    26821 gtaatgttta gcaaagctat caccaatttg tatctttttt tcatgtctat cccttctttc
    26881 ttttcaagtt cttatataaa acgctttcac ttatgtatat taacggaatt agtaacacaa
    26941 atatttatat aaaaaaatat tttacttgtc gttttggctt ttcatttttg ttataatagt
    27001 cattaattat aaatagaaag ctttttatat aaaaagagaa aggaaagtgg ttcttttgtt
    27061 aacaaacaac caatcctcca atcttgtcaa aaaaacacta acctattcca tagataccca
    27121 tcttcattta tctgctgctc cctattttcg actactaata aatgaaagcg ctaatttata
    27181 tgctgaattt gcacataaat ctatagaact agataatcct tcccttttat tcattgataa
    27241 acagcaagaa gtccaaatct tttctactca aaaggaaaca caaactttta tcattatcca
    27301 gttttatttt gatgagcaat tattagcaca tattacggga caatcttttt ctatagatca
    27361 agaaataaca tctatcatta cttttctgaa aaatgaacag ttcaaaattg attatctaaa
    27421 acatagtaag ccagctattt cctatgatca ggaaatcaat tcttttttca tactatgcca
    27481 acagatgagc attcatttgc tttccttatt agttacttat tgtctgcaaa aaattcaatt
    27541 gtcttcctta ccaaggcatt atcaacagga acttgcttta ttgacaatac cttcaaatga
    27601 gaaagtcatt cctaaaaaaa agcatattct ttattcagat gaattagttg atccaatcat
    27661 acgatattta gaagaacatc tttcagaaaa ttttttgctt gaacaattag cccaaatttt
    27721 ttttatcagt ccagccagct taaaaaaaca ttttagaaaa gtgaatggaa aaagtgttat
    27781 tagctatttt aaagaactaa aaatggaaaa agcaaaacgt ctcgtgctag aaaatgaatt
    27841 aacctataca gaaattgcag atatattagg atttcattcg atccatcatt tttcttcagc
    27901 ttttaaaaaa tatacagggt tatccccttc aaagtattat ctatccttaa aatagaaaaa
    27961 aatttttagg ttttatccac aaattatttc tctatatttt ctgtctattc aaaataaaca
    28021 ataattttcc ggcacaatta tttgctgttt tttaccataa catagtacga tcaacgtaga
    28081 aaaggagatg tttttatgaa agttgttgtc attggcggtg tagctggcgg tccttcattt
    28141 gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca ttatctatga acgcggagaa
    28201 aatatttctt atgcaagttg tgctttgcct tattatttag gaggtgtgat cactgaccgt
    28261 gactcgctga tcgaacgtac accagaaata ttgaaaacaa aaaacaacat cgatgtcttt
    28321 actaaacacg aagtaacagc aatcgatcct tctactaagc gattaacagt taaagaccta
    28381 tccacaaatg aagaaataaa aacagattac gataagttga tcatctcttc tggtgctaga
    28441 ccagattatc cggatattcc cggagttttt gaagcagaaa acggctttgt cctccgtagt
    28501 gtgacggatg cggatcgaat caaatcgttc cttgaagaaa aaaatccaca acatgtcgtc
    28561 attcttggtg caggtgttat gggtctggaa ttagctgaga atttcaagca tcgcggctta
    28621 gacgtgactt taatcgatca attgccacaa gtcgctttcc cttatgatcc agaaattgct
    28681 aatttagttt atgacaaatt gctgaaagaa ggattagccg ttcatttaga aacaagagtt
    28741 actgagatcc gtgataaagg tcgagaaata gtattatcag atggctccgt cctttctgct
    28801 gatatgctaa tttttgctgt tggtgtttct ccgaagaatg aagtggtgaa agcaaccggc
    28861 atacaattat cagatacagg tcagatcatt gttgatgacc agttacaaac caatctgccg
    28921 gacatctatg cgattggcga tattatcgaa acgactagtg tagtgactgg tcagccgatc
    28981 caaagtatgc tttccagtgc ggccaatcgt caaggacata tgctggcaga tattttaaat
    29041 ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg tcgcaaaaat ctttgatcat
    29101 acagcaagtt atgcgggaat gacagaacat gaattaaaag catcaggcat aacaaattat
    29161 aaaactgttt ttatcactcc ctttgaccat gcctatttct atccagaagc tacaagatta
    29221 aatctaaagc tgatttttga tgcagatagc ggtcgtattt taggtggaca agcatttgga
    29281 gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag caatcaccgg aaacttgaca
    29341 gtctttgatt tgcctgattt ggagttgcct tactctccac cgtattctac tacccgtgac
    29401 ccgttgaata tagctggtta tgtcgcaatc aatcaaatga cgaatatcgt agaaacgatc
    29461 aaagcaagtg atatacccga aaacgatttg aaagaagcgt tctttttaga tatacgtgaa
    29521 cctaataaag caccttctgg cagtatctcg gcaacaaaaa atattccaat gaatgagtta
    29581 cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt acattacttt cagaagagga
    29641 ttgaatactt atacttctgc ccgaatcttg gcaggtttgg gtatcaaagc gattttgatt
    29701 gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta ggagttattt ttacttgagg
    29761 agtaaaaatg aaaaagtata tagggttgtt ggttggtatg tatatagaat agaagataaa
    29821 tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt tatagtttaa gaaagtttaa
    29881 acattgcgca atttaattat tttagctgtt tcttttcata aaaaaacatg ttgtgactct
    29941 gagacaaatc atagtccata gtgatgtctc tagagtaact acgcctaata ttacgaggca
    30001 tcatattaat ctatcagcac tagtacctaa actagcatcc ctttcgaata ttttttctta
    30061 tataataaag tttgctttca ctcctctaat ctcaccaaat cgactggctt gcgttgtgtg
    30121 atactaggaa ctgattggtt tagaacgact tttctattgt aatcatattt ttcgccatcc
    30181 tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg tgtattaaat aatttaattt
    30241 ttttgttcct atttattta
//
LOCUS       DACEDM010000035        30103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEDM010000035 DACEDM010000000
VERSION     DACEDM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.035"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(193..1644)
                     /gene="cls"
                     /locus_tag="IWO50_001395"
     CDS             complement(193..1644)
                     /gene="cls"
                     /locus_tag="IWO50_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0358525.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1985..4651
                     /locus_tag="IWO50_001396"
     CDS             1985..4651
                     /locus_tag="IWO50_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0358526.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5015..5767
                     /locus_tag="IWO50_001397"
     CDS             5015..5767
                     /locus_tag="IWO50_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0358527.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5789..6943)
                     /locus_tag="IWO50_001398"
     CDS             complement(5789..6943)
                     /locus_tag="IWO50_001398"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0358528.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7128..9569
                     /locus_tag="IWO50_001399"
     CDS             7128..9569
                     /locus_tag="IWO50_001399"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0358529.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9899..10603)
                     /locus_tag="IWO50_001400"
     CDS             complement(9899..10603)
                     /locus_tag="IWO50_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358530.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10790..11035)
                     /locus_tag="IWO50_001401"
     CDS             complement(10790..11035)
                     /locus_tag="IWO50_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358531.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11052..11903)
                     /locus_tag="IWO50_001402"
     CDS             complement(11052..11903)
                     /locus_tag="IWO50_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0358532.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12069..13073
                     /locus_tag="IWO50_001403"
     CDS             12069..13073
                     /locus_tag="IWO50_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0358533.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13314..13670)
                     /locus_tag="IWO50_001404"
     CDS             complement(13314..13670)
                     /locus_tag="IWO50_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0358534.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14038..15225
                     /gene="tuf"
                     /locus_tag="IWO50_001405"
     CDS             14038..15225
                     /gene="tuf"
                     /locus_tag="IWO50_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0358535.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15413..18004)
                     /gene="mprF"
                     /locus_tag="IWO50_001406"
     CDS             complement(15413..18004)
                     /gene="mprF"
                     /locus_tag="IWO50_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0358536.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18030..18749)
                     /locus_tag="IWO50_001407"
     CDS             complement(18030..18749)
                     /locus_tag="IWO50_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0358537.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            19069..19590
                     /locus_tag="IWO50_001408"
     CDS             19069..19590
                     /locus_tag="IWO50_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0358538.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            19649..20509
                     /locus_tag="IWO50_001409"
     CDS             19649..20509
                     /locus_tag="IWO50_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0358539.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20616..23009
                     /locus_tag="IWO50_001410"
     CDS             20616..23009
                     /locus_tag="IWO50_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0358540.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23191..25032
                     /locus_tag="IWO50_001411"
     CDS             23191..25032
                     /locus_tag="IWO50_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0358541.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25056..26165
                     /gene="rpoD"
                     /locus_tag="IWO50_001412"
     CDS             25056..26165
                     /gene="rpoD"
                     /locus_tag="IWO50_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0358542.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            26385..28484
                     /locus_tag="IWO50_001413"
     CDS             26385..28484
                     /locus_tag="IWO50_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0358543.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            28680..29543
                     /locus_tag="IWO50_001414"
     CDS             28680..29543
                     /locus_tag="IWO50_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0358544.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            29648..>30103
                     /locus_tag="IWO50_001415"
     CDS             29648..>30103
                     /locus_tag="IWO50_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0358545.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT        10006 a         5313 c         6259 g         8525 t
ORIGIN      
        1 gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag cttctcatgt
       61 ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tgttcggaag
      121 aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt tttctctact
      181 tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct tgaataatag
      241 ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg tctcatcttc
      301 tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag cacttgtttc
      361 aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc agatttcatc
      421 atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga tttgtacatt
      481 ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag gatgatccgg
      541 tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt ttaaagcagc
      601 aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt ttttagcaga
      661 agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg gtccgcttga
      721 aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt ttttaaagaa
      781 ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc agtctgttaa
      841 gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg tatcgcgcca
      901 atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac caccggtata
      961 actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca atcggaaacg
     1021 aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt tgattagacg
     1081 attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata ttcgaacttc
     1141 cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga tattatctgt
     1201 cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat cttgaagcag
     1261 tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc ttgtcagcgg
     1321 catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat cgcccatgtc
     1381 aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta attctttcaa
     1441 gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga aaaataaata
     1501 taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc aaatggctgc
     1561 tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta atagataaag
     1621 ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata acattagtat
     1681 agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct ttcatgaaaa
     1741 tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag gatattagta
     1801 cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat atcaacagta
     1861 agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac aaatgcgtat
     1921 ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa atgggggatg
     1981 aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga tcgactcgtt
     2041 gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga aggaaaaatt
     2101 agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt ggaaattgtt
     2161 cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac tgattcagat
     2221 ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt tgaattctat
     2281 aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga aaaacctttc
     2341 agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg caaacgaact
     2401 tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg atggacgttt
     2461 actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt cgacttctgc
     2521 agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta caaatatggt
     2581 attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt caactgggaa
     2641 taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc aagtcactcc
     2701 gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt tggcattgtc
     2761 tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta caggagatat
     2821 gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag ctgctattcc
     2881 agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata aaatggcaaa
     2941 acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga caagtatcat
     3001 ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg actacttttt
     3061 aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag ctgacgaacg
     3121 tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag acggtactaa
     3181 attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa accagcctta
     3241 tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact cagatcgaaa
     3301 attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta aaggcggacc
     3361 agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg ttccgatgac
     3421 ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac gcgcattacg
     3481 tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg aagatgaaga
     3541 cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg aagtgactca
     3601 ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg gtgaccacaa
     3661 aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa acctggcttt
     3721 gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt tagaaaaagt
     3781 cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg cttggcaaaa
     3841 taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag cattgaaaca
     3901 agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg cagcagcgat
     3961 ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag gacgtaatgt
     4021 gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag gtgcgatcat
     4081 tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat tacaactgtt
     4141 gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag aaaaagctga
     4201 acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta cggacaaaac
     4261 attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct ttgctcaatg
     4321 gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa cattgatttg
     4381 gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta tccaagcagg
     4441 gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc tttactgctt
     4501 aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg gattgacaga
     4561 agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa tcactaaatt
     4621 gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt gagcctgccg
     4681 tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt gttgaatatt
     4741 gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt tgtaaaatca
     4801 gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg aaagagggac
     4861 tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa agccaatcat
     4921 tttattattc atcaaaagag atagaataga taaaagagga gaatgattta ttccgaaaag
     4981 tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat gggatgaatt
     5041 tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg aagatgtaaa
     5101 aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg ttgctggagg
     5161 atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga tcgatttttc
     5221 ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc caaatgtttc
     5281 ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg aaatggtttt
     5341 tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga tgaaaaagtc
     5401 aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt tttatcctat
     5461 cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg taatggatca
     5521 atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt ttacttattt
     5581 tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt acgcaaaaga
     5641 tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa tccttagtac
     5701 gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa cctttcacga
     5761 ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata tcattttcga
     5821 tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg tgttcaggaa
     5881 gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga gaatctagca
     5941 tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga taacctgtaa
     6001 aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct cctggctgta
     6061 gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca gtgtaaagat
     6121 taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga cgttcctgat
     6181 tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc ttagtatacg
     6241 gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat tctttatgtc
     6301 gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga ccatcaacaa
     6361 caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc agaaatacgc
     6421 tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca acagcaccga
     6481 caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca cttgctatga
     6541 ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc aaagctgcgg
     6601 tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg attttcagta
     6661 aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct gttccgacga
     6721 tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca gcatttgctc
     6781 ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga ccaatcccta
     6841 tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata tctaaatata
     6901 aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca cctctttact
     6961 tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa tataaaaaag
     7021 agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa caagagctat
     7081 ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg aaagaagtcg
     7141 ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta agtccattta
     7201 ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca aaactgaaaa
     7261 aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat ggtcaaaatg
     7321 ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca atgacgatca
     7381 atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg atccaattag
     7441 gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca cctatgctga
     7501 aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg gtcaacgacg
     7561 gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat gttgcaacac
     7621 aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag ttgaaagcag
     7681 cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag atttctgatg
     7741 aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa aaactgggca
     7801 cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct tccccgttga
     7861 atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat aataatctgc
     7921 cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt atcatgggaa
     7981 tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat ttatcaacaa
     8041 tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt agccaagaac
     8101 tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta ggccatccaa
     8161 ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt cgtgaacaga
     8221 agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca gtcttgttag
     8281 aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat cagcttacgc
     8341 catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa actgcgttga
     8401 tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa aaccaagtga
     8461 gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc aagaaaaaat
     8521 ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat ggtgcaaaaa
     8581 tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa atcgttctat
     8641 caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa gaagagctga
     8701 ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt gttcaagata
     8761 tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat tttttagtgg
     8821 atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt gtggcaaaca
     8881 agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt aattttgcta
     8941 cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg agaaacaaag
     9001 aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg aagcttgacc
     9061 cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct gtagtggcag
     9121 ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg gcaagaaatg
     9181 gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt ggaaaactaa
     9241 cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca aaagcaaaag
     9301 catctttagc tatgttagat attgattcag ccaaggaact agcacaagta attgctgcag
     9361 tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga atccaaaaag
     9421 gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt ggtgaggaaa
     9481 tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag acagctatac
     9541 aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga ataaagcaag
     9601 atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa aatatgttta
     9661 ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa ctccaagata
     9721 tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt gttggacagg
     9781 tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct gtagaaatta
     9841 ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa tagaattttt
     9901 atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa gtcaatttga
     9961 ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta gtcatagaaa
    10021 tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact tcatgttcat
    10081 tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca gcaaaactta
    10141 aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt ggtttttccg
    10201 ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca gcttgcgcag
    10261 ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca ataagaagat
    10321 tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt aatacagtat
    10381 tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc ccagctaatc
    10441 cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc aaactataac
    10501 gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca tccaatgaag
    10561 gacggaattg atttaataat ttttgtttct gttttttctt catatgttct ctccttcgct
    10621 catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg caaaaaaact
    10681 tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt gaaagaaaaa
    10741 taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc tattttttcg
    10801 gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg tgccagggtt
    10861 tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga tcatgtgtcc
    10921 agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca aaatcggaat
    10981 gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga tccataaaat
    11041 cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac tacaccatct
    11101 aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa ttcagtattt
    11161 tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg ttgcagcgac
    11221 caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg tttcatttca
    11281 ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat catatcatgt
    11341 ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa aacttgattg
    11401 accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg taagaaattg
    11461 ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc tcgccatgta
    11521 tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca gtggatcaaa
    11581 aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga agacatcaca
    11641 agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc ttctctagga
    11701 attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc ttgggcagta
    11761 tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc gacatcccca
    11821 tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt tgatagacga
    11881 taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca actataagtg
    11941 tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt tttgtttaaa
    12001 agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag acagaaggga
    12061 atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac agagaaactg
    12121 gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt cgtgattttc
    12181 ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa ctagcagaag
    12241 aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa gcaagtgaag
    12301 caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct agtgcagatg
    12361 tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg aaagtcaatt
    12421 atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac aaccgcttga
    12481 ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca ttcgctgtga
    12541 atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct ggcaaaaaag
    12601 agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta gaagaaaatg
    12661 taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca gaagaatttg
    12721 atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa ggtcggttgg
    12781 atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa atggctttta
    12841 aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa gaagtacaag
    12901 aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg atcgaactga
    12961 tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat aatccggatg
    13021 gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta taaaaatgac
    13081 tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca agagaaggat
    13141 cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac acttttgtct
    13201 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat ttcttggagt
    13261 taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat ttactaatta
    13321 tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc ccagcttaat
    13381 acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa tggatgttct
    13441 ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc cgcttgcttg
    13501 taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat ttcgccattc
    13561 gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc tgcaaaactt
    13621 ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat gtacttcacc
    13681 tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt ggcaacagct
    13741 gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta aggactatct
    13801 tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt tcgcgtataa
    13861 gacagacgag tgctcttagt tgactttgac caatattgac gtataataag cttagttcga
    13921 atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa gactataata
    13981 aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt gtaaattatg
    14041 gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat tggacacgtt
    14101 gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa aaaaggccta
    14161 gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga acgtgggatt
    14221 acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc gcatattgat
    14281 gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca aatggatggt
    14341 gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga acacatttta
    14401 ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt tgatttagtc
    14461 gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt gagcgaatat
    14521 ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc attgcaaggc
    14581 gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga atatatccca
    14641 acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt cttctcaatt
    14701 acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg tgtcggtgat
    14761 gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac aggtgtagaa
    14821 atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt gttgttacgg
    14881 gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg ttccattact
    14941 ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg tggacgtcat
    15001 actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga tgttacagga
    15061 acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt gacgattgat
    15121 gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat tcgtgaaggc
    15181 ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac gcgactcgaa
    15241 tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat ttctgtttgg
    15301 accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac tttcgtttta
    15361 cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt ttttaatact
    15421 ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca actggcgcat
    15481 tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta gaatatagag
    15541 tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat ccatggaaag
    15601 aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt aaaaaactct
    15661 ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat tggatatgtt
    15721 cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca gaaggtgctt
    15781 ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg taagtaggca
    15841 tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca ataggcgctc
    15901 tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt cgtgaaccca
    15961 accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat ggtggttgta
    16021 agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga gtgcccttca
    16081 tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc cccatcttaa
    16141 tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt tcgtagaaaa
    16201 ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct tctggaaaat
    16261 cttctttctt accagaagga tcacccatta caatacattt attattgaag caagcgaatt
    16321 ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta tcttttaaga
    16381 agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg gccttttctt
    16441 cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca aatagaacga
    16501 tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt tcagaaggaa
    16561 ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atccggcaga ttgtataccc
    16621 caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc gtacgccact
    16681 cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga atgatcagaa
    16741 gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa acataaagta
    16801 aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt gctgcgatcc
    16861 ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg ataataaaat
    16921 gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct tgcgggatag
    16981 tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg attttatgcg
    17041 caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa gtggaaccga
    17101 tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag aacaggcgat
    17161 agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac aacccgataa
    17221 tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt cctatcactg
    17281 aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc attaaataac
    17341 caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact agcaatagct
    17401 gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa gtagtaaata
    17461 aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat tgcttcaaaa
    17521 aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa atagaaagtc
    17581 cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg ctctctttct
    17641 tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa ccggcaatat
    17701 tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc tgcttttgtt
    17761 caagcatccg attcaaaata atatcataac caaccatagg taacacagag ataagaccaa
    17821 tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct cctaattgct
    17881 ggatagatag agttttaccg atggacatca attcaccgac aataatgaca atgacagaaa
    17941 tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg gtatttttta
    18001 acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg aagaacttct
    18061 cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt cacaagcaca
    18121 aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt gattacatct
    18181 ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt ttctgtaagc
    18241 ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact tgggcgaaat
    18301 aaagagagta accataccca tgcataatga atattactga tgactctggc agaaaaacga
    18361 acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt tttagggaag
    18421 ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa atctgcctga
    18481 tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg aaaattcaac
    18541 atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata cacggtatag
    18601 tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc attgccatgc
    18661 agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct cccatctggc
    18721 atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag tataacgatt
    18781 atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg taagatgtca
    18841 aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg aagaaatagc
    18901 caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg tgatgaaatg
    18961 aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct gacggttatt
    19021 ggtgcggtgt tgtttttatg aggaagaact tatcgttcgg taaaacgagt ggaaagctat
    19081 caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga gaatttgatc
    19141 aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca gagctcggaa
    19201 agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga tcaaggtgtc
    19261 aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt atggacagct
    19321 gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca tggcggagaa
    19381 aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact tttaggaaat
    19441 gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa tggcggctat
    19501 ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa tgaaaaaata
    19561 atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat catgaataaa
    19621 attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg aaaatcaaga
    19681 agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt ttatcggttc
    19741 aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg gcatgtatga
    19801 gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg ttttgttttt
    19861 tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt tagtcagttt
    19921 tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta tcgctggaac
    19981 tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg atgtgatcat
    20041 catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc aattatttga
    20101 cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga ttggtttggg
    20161 tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat tgtataaagc
    20221 gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg gaattggtgg
    20281 gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa aggcatatag
    20341 caagctattc catttcattt taggtgtagt ttttgcttca acgattatga tcattcctac
    20401 cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt ttatagccgg
    20461 tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa gatagattga
    20521 taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt tgtaaaatca
    20581 ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg gaacacgcta
    20641 gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag aaaatgaatg
    20701 gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat gactaccagg
    20761 aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg ttattcaatt
    20821 ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc aacatggcgt
    20881 atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata catgcaagaa
    20941 atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc cgggacggta
    21001 aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa atcggagcag
    21061 gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca aatgaagctc
    21121 ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg ctttctggaa
    21181 gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca gataattatg
    21241 ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa cttgtagatt
    21301 cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg atcatactat
    21361 ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt gcatctgcag
    21421 cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt gcattaacga
    21481 caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat tcaattgaaa
    21541 cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact gaagggaaaa
    21601 tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc ccagaaatta
    21661 tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct atgagagatt
    21721 attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct tctgatcgca
    21781 aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg cctgaacgtt
    21841 tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat ggctatcgcg
    21901 tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca gcatttgttg
    21961 agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa gaaacactag
    22021 cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat ccttttactg
    22081 tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc gatctttctc
    22141 aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt tttggacgag
    22201 tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc agaacggttg
    22261 caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat tgcagcattg
    22321 ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg ttggactctg
    22381 attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac aatgtgacga
    22441 aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg attatatgtg
    22501 ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt gatttagcga
    22561 ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa atcacttacc
    22621 gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta gtagtgaata
    22681 tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa actactactt
    22741 tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct tgttatccct
    22801 tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat gtagctgcaa
    22861 tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta ggatacttct
    22921 ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct gatattcaaa
    22981 atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt gttatacttg
    23041 aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta aaatcgcact
    23101 gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct gttaaaccca
    23161 aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca agaggttatc
    23221 gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt ccagttgaaa
    23281 aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac accgtctttt
    23341 tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg cggaaacgtg
    23401 tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt aaaagtggca
    23461 gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc tgtccaaaat
    23521 cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga aatctatcat
    23581 cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca aagtcgtggg
    23641 ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga gcgttctttt
    23701 cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg ctctggattg
    23761 tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt gatgtttcca
    23821 cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc acctgaaaaa
    23881 gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact gttcaataaa
    23941 agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa cggagaaatt
    24001 ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat caaaactgga
    24061 ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga aaagctgacg
    24121 aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac caatcgaggg
    24181 attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt tccagaaaag
    24241 cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga attagtggct
    24301 catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg gaaaaattta
    24361 gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact tagccgagtt
    24421 gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt tcagatcggc
    24481 agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca aaatcgtcaa
    24541 gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg gaaaaagaga
    24601 cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt taaagaagtt
    24661 tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc atatgcagaa
    24721 gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct tgcagagttt
    24781 ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga atatttaccg
    24841 gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat cagcaaatct
    24901 ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag acgaactgga
    24961 aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa acagttaaaa
    25021 caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca gataaaaaaa
    25081 cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga caagttgtat
    25141 atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat gaaatggaca
    25201 aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat ggtgatccat
    25261 caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca gaagatcttt
    25321 ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa gaaattggcc
    25381 gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc gaagaaggcg
    25441 accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta agtatcgcta
    25501 aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga aacatgggac
    25561 tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca acctatgcta
    25621 catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt acgatccgta
    25681 ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt caattgctgc
    25741 aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat cttccaacag
    25801 aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa acgccaatcg
    25861 gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca acaagtccag
    25921 cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta gatacattga
    25981 ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc cggacgagaa
    26041 cattggaaga agtagggaaa gtatttggtg ttactcggga acgtattcgt cagatcgagg
    26101 ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa gatttcttag
    26161 aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt tattttaaaa
    26221 gaaaaaaagc ttgagactac gcagtctcaa gctttttttt ctataggaag gaaattcatt
    26281 cattattttt aagtaaatcc taaaaataag ctattggcgt gacgtataga atatgataga
    26341 ttaaatggta gcgtaaacct agtaaaggag ccgatcgcta agtgatgaaa aaatgtccaa
    26401 attgcggatc gaaaaaaatg gagcagaata ctatctgtcc agactgcggt catcaaataa
    26461 ctgcagaaga tcagcaaata aaagaatcaa ttgaagaaac aaatacagat gcagctatgc
    26521 aggataaatc aactgaagat gcagtctttc ctgatacaga attaaacgat ccaatcgaat
    26581 ggtcggaatt gaaagattta cctctagaat ctgtaatgga attattcgaa aaagcagaac
    26641 cagaagacga agaaccacct gctcacgatc aagtaactaa tatagagaaa aatgaacaga
    26701 agaaagcaac tgttcatgag acttcggaag aatcaggaaa gaatcaagaa gaaacgcttg
    26761 aatcaaaaca aaaaagaaga gtagctgaac tgaaagaaat cgtcgataat gaggaagaaa
    26821 attcgatact atccgcttat atcaaagcac atcgtgaaga tacaaaagaa gagcacgcga
    26881 aagagctgct caaaatgatc agtgaaaaaa ttgcacagga aaacaaagta gagccagaaa
    26941 aggaacaaga aactgaaaaa gactctgata gagaaaacgc cacaggtaaa gaaacagtaa
    27001 aaataaaaga cgtaaaaacg gatatcacag aaaaaactga agtatttact gaaaaacaat
    27061 tagaaaagga aaaaacgaat aaactgccgg aagcagagaa aaaagaaata ctcgaaaaca
    27121 cagacaaaaa gtcaacagag cccgatgaaa taaatagtga atccgatgct gaaaatcaat
    27181 ctgtaagcaa agagggtact gatcttgaag caaaaataga aaaagaagag aaccagccta
    27241 agctgtttga agcagaagcg aaatcaataa caatcgtgcc agatactgaa aaaacacatc
    27301 aagaatcaaa cttagaaaaa acagatccag ataaagcgca aaagaaaaaa tcaaaaaaag
    27361 taccttatct cgttttagca gcagtacttc tactcggtgg aggaagctgg gcttattatg
    27421 accatcatca aaaagtagaa gccgaagtag cagcagaagc aaaaagacag caacaaatgg
    27481 aagaacttca tgagaaactt gctgcctttt atacagatga tcaccatcaa tttattcgtg
    27541 cagatatgat tcatcaagat ttatcaaaac taaaaaatga tttatccagt cttaagaatg
    27601 aaaaaggata ttcagagtta gaaacaactt accaagatat ccaatctaag atccgagata
    27661 tccaaaaagt gaatgagtgg ttcatagcac ctgccatcgt cgatgatcat ctagcagatg
    27721 acatcaagtt gaaagcagat aaagagatcc aagcaatcga aacggataac acagattttg
    27781 gaaaattgat tcaacaagca caaacagaag ctaaaaatca atatgctgaa ttgcaaacag
    27841 ctaaagaaaa aacagctata gtctttaaag acggaaaagt agtggattca gcaacacgcg
    27901 accaatacaa gacggcaaaa gaagcagtag aaaaagtgaa aaatacagat ctatctgctc
    27961 agttgaatga acaattgaaa caagtagata agatgctcac tgataaagag aaaaaagaag
    28021 aagaggaaaa gaaaaaagca gaagaagcga aaaaagcagc tgaagaacaa gcaaaacaag
    28081 ccgctcaagc agcagctgca gctcaagccg ctcaacaagc tgcggtcgca caagcagcac
    28141 agcaagaagc ggcatcctcg accccttcct cttcaaactc agcgaatcag ccaatcaaat
    28201 ctgccagagc aagtgatatt gccgatgcat ctaatccagc atggacgtgg gcaccaggtg
    28261 tccaaagcaa agtgattgct acatgtatcc aacgtggata tatcgttgaa ggaggttaca
    28321 gattagaaaa agccagaatc gaaaatgggg aaggctatta taatctatat gccacttcaa
    28381 caaaatctgc attgatgaaa ggcattggag aaagtgcttt gccattttac atcgtaacca
    28441 tcaactgtaa aactggatgg ttcggaggaa atgggagcaa ttaattttag gcaaatgaaa
    28501 gaggctgtga gcctgccgtt taacaccgtt tattcgaaaa aggggagtat gacacaactg
    28561 ttgtcatact ccttttttca cctttccaaa ccaaggctca gctattcaag tagataacat
    28621 tatgttatga tagcaaagaa atgtattaag aaaacctttc actatagagg gagaaaaaaa
    28681 tgaacgattt attagcaact atatttacag gtattattat tgatgaaaat gaaaaaaact
    28741 actttgtcca aaaaaacgga caaacctttg gcttaagcaa atcagaaggt gaacacaaaa
    28801 tcggtgaagc agttgaagga ttcggttacc aaaatcaaaa acatgaaaat cggatcacta
    28861 cacaaattcc taaaagccgt atcggtcatt acgcttttgg aatcgttact gctacacgaa
    28921 aagatttagg tgcgtttgtc gatatcggtc ttccagataa agactttgtc gtatctcttg
    28981 atgaattgcc gacaatgaca gagctatggc ctaaaaaagg ggatcgcttg atgatttccc
    29041 ttagagttga tgcaaaagac cgtatttggg gcagtttagc agatgaaaaa atattcaaat
    29101 ctttaagcaa atatggaaca gaagaaatga aaaacaaaga tgtgatagga acagcatacc
    29161 gcttgaaatt aacaggtacg tacgttttaa cggaagattt ctatcttggg tttatccatc
    29221 cttctgaacg atataatgaa cctcgtctag gcgagcagat aaaaggtcga gtgattggtg
    29281 tccgaccaga cggtgtactg aatctttcat tgaagccaag agcatatgaa gctatatcag
    29341 atgatgcaca aatgatttta accattttag aacgttctgc agatcagcaa atcaacttta
    29401 cagataaatc ggatccagaa gaaatcatga aagcttttgg tatcagcaaa ggagcattta
    29461 agcgcgcatt aggtaatctt ttgaaacaag gattgattac acaagaaaac ggtgtgacaa
    29521 aactagtcaa aaaatctaat tgagaatccc ttgcatgttt ttcttaacta gcttataata
    29581 attttaatta acatagtgtt atatcttttt ttatttgtaa ttattataaa ttgttggggt
    29641 tgagtaaatg gtcatgaatg ctatttcggc gatgaaaaaa acaaaacagc agctacatga
    29701 agcggggttt aagctgacac cgcaaagaga agctactctg cttgtgcttt tggaaaatga
    29761 gaaagagcat ttatctgcag aagaaatctt ctttttagtg aaaaaaaaga gtccggaaat
    29821 tggacttgct actgtttatc gaacccttga aatcttgaca gaattgaaaa ttgtcgataa
    29881 agtaagtttt aatgatggat tagcccgtta tgatttgaga aaagaaggcg ccaaacattt
    29941 ccatcatcat ttactatgct tgaactgcgg cacgatcgag gaagtagaag aggatctttt
    30001 acttgaagtg gagcaagtag tagaagaacg ttatcatttt ttagtaaaag atcatcggct
    30061 aacgttccat ggaatctgtc aggaatgcta tgaaaaagaa aaa
//
LOCUS       DACEDM010000036        29961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEDM010000036 DACEDM010000000
VERSION     DACEDM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.036"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IWO50_001416"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IWO50_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0358546.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2945..3379)
                     /locus_tag="IWO50_001417"
     CDS             complement(2945..3379)
                     /locus_tag="IWO50_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358547.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3396..4601)
                     /locus_tag="IWO50_001418"
     CDS             complement(3396..4601)
                     /locus_tag="IWO50_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0358548.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5295..5681
                     /locus_tag="IWO50_001419"
     CDS             5295..5681
                     /locus_tag="IWO50_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0358549.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5775..5966
                     /locus_tag="IWO50_001420"
     CDS             5775..5966
                     /locus_tag="IWO50_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0358550.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6480..6812
                     /locus_tag="IWO50_001421"
     CDS             6480..6812
                     /locus_tag="IWO50_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0358551.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7108..7263
                     /locus_tag="IWO50_001422"
     CDS             7108..7263
                     /locus_tag="IWO50_001422"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0358552.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7539..8879
                     /locus_tag="IWO50_001423"
                     /pseudo
     CDS             7539..8879
                     /locus_tag="IWO50_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9120..10736)
                     /locus_tag="IWO50_001424"
     CDS             complement(9120..10736)
                     /locus_tag="IWO50_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0358553.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10854..11072
                     /locus_tag="IWO50_001425"
     CDS             10854..11072
                     /locus_tag="IWO50_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0358554.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11267..11728
                     /locus_tag="IWO50_001426"
     CDS             11267..11728
                     /locus_tag="IWO50_001426"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0358555.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11921..12160)
                     /locus_tag="IWO50_001427"
     CDS             complement(11921..12160)
                     /locus_tag="IWO50_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358556.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12184..13707)
                     /locus_tag="IWO50_001428"
     CDS             complement(12184..13707)
                     /locus_tag="IWO50_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0358557.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13725..13847)
                     /locus_tag="IWO50_001429"
     CDS             complement(13725..13847)
                     /locus_tag="IWO50_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0358558.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13877..14860)
                     /locus_tag="IWO50_001430"
     CDS             complement(13877..14860)
                     /locus_tag="IWO50_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0358559.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14884..15033)
                     /gene="rpmG"
                     /locus_tag="IWO50_001431"
     CDS             complement(14884..15033)
                     /gene="rpmG"
                     /locus_tag="IWO50_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0358560.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15054..15431)
                     /locus_tag="IWO50_001432"
     CDS             complement(15054..15431)
                     /locus_tag="IWO50_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358561.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15463..15642)
                     /gene="rpmF"
                     /locus_tag="IWO50_001433"
     CDS             complement(15463..15642)
                     /gene="rpmF"
                     /locus_tag="IWO50_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0358562.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15657..15926)
                     /gene="rpsN"
                     /locus_tag="IWO50_001434"
     CDS             complement(15657..15926)
                     /gene="rpsN"
                     /locus_tag="IWO50_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0358563.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16449..18221)
                     /locus_tag="IWO50_001435"
     CDS             complement(16449..18221)
                     /locus_tag="IWO50_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358564.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18175..19929)
                     /locus_tag="IWO50_001436"
     CDS             complement(18175..19929)
                     /locus_tag="IWO50_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358565.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20039..20608)
                     /locus_tag="IWO50_001437"
     CDS             complement(20039..20608)
                     /locus_tag="IWO50_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0358566.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20626..22050)
                     /locus_tag="IWO50_001438"
     CDS             complement(20626..22050)
                     /locus_tag="IWO50_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0358567.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(22052..22720)
                     /locus_tag="IWO50_001439"
     CDS             complement(22052..22720)
                     /locus_tag="IWO50_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0358568.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22850..23434)
                     /locus_tag="IWO50_001440"
     CDS             complement(22850..23434)
                     /locus_tag="IWO50_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0358569.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23765..23998)
                     /locus_tag="IWO50_001441"
     CDS             complement(23765..23998)
                     /locus_tag="IWO50_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0358570.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24013..24963)
                     /locus_tag="IWO50_001442"
     CDS             complement(24013..24963)
                     /locus_tag="IWO50_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0358571.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24960..25802)
                     /locus_tag="IWO50_001443"
     CDS             complement(24960..25802)
                     /locus_tag="IWO50_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0358572.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25804..26523)
                     /locus_tag="IWO50_001444"
     CDS             complement(25804..26523)
                     /locus_tag="IWO50_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358573.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27215..27373
                     /locus_tag="IWO50_001445"
     CDS             27215..27373
                     /locus_tag="IWO50_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0358574.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27445..28671
                     /locus_tag="IWO50_001446"
     CDS             27445..28671
                     /locus_tag="IWO50_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0358575.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28759..29151)
                     /gene="rpsI"
                     /locus_tag="IWO50_001447"
     CDS             complement(28759..29151)
                     /gene="rpsI"
                     /locus_tag="IWO50_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0358576.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29167..29610)
                     /gene="rplM"
                     /locus_tag="IWO50_001448"
     CDS             complement(29167..29610)
                     /gene="rplM"
                     /locus_tag="IWO50_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0358577.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         9807 a         5531 c         4357 g        10266 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta ctctacatga
     2941 ataattaatt gctttcactc aaatacttat aatctattaa cttgtagttg tttaactttt
     3001 ctgaaaaagt tagacccacg taataatctt cccagctttc tctttcaatt aactgatcca
     3061 atttttgata atccataaat tcatcatgta tcaaaaagaa taaataactt gatattttag
     3121 catctagctt aatcagatta aataatgttt gtttaaaatt gctaccatta atatgactga
     3181 tagattctct agcttcttca cataaacttt ttactttatt aattactact ttgtcatatt
     3241 catttaacac actattatta ccattactta gacgctcaga ttctacgaaa caactcaaat
     3301 cagaaatttc ccaaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3361 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattgtcc
     3421 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3481 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3541 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3601 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3661 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3721 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3781 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3841 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3901 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3961 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     4021 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4081 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagattttct
     4141 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4201 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4261 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4321 aaaactttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4381 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4441 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4501 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4561 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4621 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4681 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4741 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4801 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4861 aggaattttt cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat
     4921 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4981 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     5041 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5101 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5161 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5221 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5281 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5341 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5401 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5461 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5521 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttgata
     5581 aaactgataa agaaattgga aaggttctgg ggattacaag acagggtgcg acaaatttaa
     5641 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5701 tttgtatgct tctttgctcg ttctcctcct tcccaaagaa atagattata agtccatggg
     5761 aagaaggtag atttatgaat tctttaaaag aagatttcat tttagcaaag gctggaaatg
     5821 aagaagctgt tgaggcaatt ctcaaaagat ttagctcttt gattcataag caatcctgga
     5881 gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata tatctagcta
     5941 tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat aagtaaaaag
     6001 atagtttcaa atttcgaaca ataaatttga aactatcttc tttataatta taaagtatcg
     6061 attttacagc gttctataat gattcataga atctaaaacg aatgatttta attatcaacg
     6121 ttttttaatt cgtttttagc ttaaaaataa cttttgtcac agtctcactc ctaaaattac
     6181 tttatttacg tatgaagtgg gcaggaggtc gaatccagaa ctgggctatg gtacttcaac
     6241 gaggacttct cctctagaat tgaaaagcat gcgatttacc ttccgtaacg acggtagatt
     6301 tattcaacta tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa gagaattttg
     6361 gttgttaaaa actcttatct atcaacttcc acagtttagt tgacaaaccc tgatactaag
     6421 ttgggctatg atacactttt cttgactatc agttacgtta ccggaaggaa tgaactctat
     6481 tgagaaaaac atatgatcgt aaatttaaac taaagatttc acaggatatc cttgagaaaa
     6541 aagtaactac caagaaaatt gctgaagaat acaatatctc tcgtccaaca atttcccgat
     6601 gggtttcaga atatcgtcga tacgggaaaa atgcttttgc tggacaagga aaaagattac
     6661 ctgataaagc agatttctac attcttgaac aggaaaataa acgactaaaa gaggaagacg
     6721 agtgttctac gaacacaaag atcgatacgg atctccaggg atcactcaaa aactttacga
     6781 tgaaggaatc gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg
     6841 cacaagagga tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat
     6901 ggtaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt ttgggtgatg
     6961 gatattactt acattccatg taatgacggt cgcctctatt tgtccaccta tattgactta
     7021 gcaactcgta ttctacgtta ctatatggta gattcacaca tgaaaaaaga gattgtcgtc
     7081 cgtccattac aaatttatta aggagaattg cctacggtaa ttcattccga tagaggtagc
     7141 cagcatcggt cttttattta tcaagagctg ttgaacgacc accacatcac acattcagtg
     7201 agccaaactg ggactcctgt tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa
     7261 tgagaattaa tcgactccaa caaatataaa agtaaaatag agatgaaggt gttaatccaa
     7321 gattatttag aagactacta cccaaacaaa agaatccata caaaatttat gatgactcct
     7381 tgacaatttg aagcgaagaa aataagggat ctataatatc tagtgttggt gacgccttct
     7441 gttgccaata ctttttcttt tttctaataa acgcaaacag acaccttaaa atcctttaga
     7501 ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat gtcctattca gagtttacca
     7561 aagagttact agatattcta gacctaaatc ttatctttca tgaagatgcc tttcgcaaag
     7621 agcgaatcaa tgacgaaaca tgttttgttt ttgatggcac attgacctat caaccggagg
     7681 aatgctttca tcgccactat caaaatgaac aaacgatcat taaatagggc tggaaaaaag
     7741 tatccatctt attgaatgat gtcagtaact acaagaccat tctgcggatt aacaaacagc
     7801 gcttcaaatg taaacactgt ggaaagactt ttttagcaga agattcagtt agtgatcgtc
     7861 gctgctccat tgctcgcaga gtcaaacaag ccattcttga gctattaagc gaacctcttt
     7921 ctatgtcttt gatcgcacgt atgaagcaca tttctccgac cactgtcatt cgaatacttc
     7981 gcagccttcg gcctaaaacc atttctttga gccagctgtt accagaagtg gtgtgtttcg
     8041 atgaattcaa atcagtgaaa aatgtctcgg gagccatgag ctttgtcatg atggacggca
     8101 agacccatca actgattgat attgtggaaa atcggcaact caatccttta cgcgactatt
     8161 tcttgcgcta ccctataaaa gtccgtgaac aagtgcgtct agtggtttca gatttctata
     8221 caccctatcg cacattggtg aaagagtgtt ttccgaatgc ccgaatcgtc gccgatcgct
     8281 ttcatattag ccaacacatt ggtcgggctt ttaccaacca ccgaattcaa gtaatgaaga
     8341 cctttaaaaa aggggatcgt cggtcgaagc acttaaagaa atactggaaa ctacttcaaa
     8401 agaatgcgtg ggaattgaaa ggtcagcatc gctactggcg tccgtcattt cgagatcatc
     8461 tgacagaagc agagatagtc gatcggctat tatcttatga tgattcctta aagcgaggct
     8521 acgaagtcta ccaggatttt ctttccgtta tcagaagaca agatgttcct gaattcgtcg
     8581 cgctcctaaa agaagactac aaggagttgc cagagcacta ccaaccagtg ttcacgacct
     8641 tcaagaaata ccaaacagaa atcaaacgag cgttgcgcgt tccctattcc aacggcccta
     8701 tagaatgttt gaataaccat atcaaagtac taaaacgaat tgcctatggc tatagaaact
     8761 ttcaaaacta ccgagaacga atctttttat atcgaggaaa atattttaag aaaactaaga
     8821 atactaccca actgactaaa gccagaacaa caacacggct agacaagata gctgtctagc
     8881 cgtgtgagta tctcgattat tctattgaat ttcttcacca acactcattg acgaagagcc
     8941 aaaataagta atctaaccga ctgttaacta aaccattgca ggtcccgtta aagatacttg
     9001 tgcctgttgt caccgcttct ttttcttgtc cacaagcgct taacacactt aataaagccc
     9061 ctgctattat taataataat atctttttct tattaaaaac ctcctcatct atttccagat
     9121 tagtaaatgt agtaatctgt gctttctttt ttctcaatac taataactaa cttatggggt
     9181 aaacaaatac tcgtttgacc gggttttgag atccatccag ttctaaccgc gatttgatca
     9241 ggactattat cttctttaac tcgaattctt ccatctttaa tctcaacaat attatattgt
     9301 cccttcgcag gatgataatt cacgagttta tgattgatct tatccaaatc gaagcgatcc
     9361 atttccttgc catcaattcg aacgatggca tatttgatag catcttcagt tgtttcattc
     9421 tccttttcta tagagaatac aaataaggga ataaaagagg taataatcaa aatgccaata
     9481 attatgacat cgcctatctt gacaccagct tttcttaaaa atttcattaa cgatatctgg
     9541 ctaaacatat tagacttttt ccatggaaat ggtgaaattt ttcggaaatt atctgcatta
     9601 agagaatcag ttaaacaacc ctcaagttgt tccttaatac attgttgatc catcttcata
     9661 ccaataaaaa ctagtttatt aatgcgttca ccaaacaacg gatgccaggc ttccttcaat
     9721 tcgggatttt tttgtataat catttgacac tcaattttag gtaaactagc tacccaataa
     9781 tttaatgatt caatactttt atagctaccc gatgtttcaa agatcgtcga aaaatcacta
     9841 tgttgggcaa accaaagaaa cccttttgct cttgtaatat tgccaggaaa atttttatcc
     9901 gtaagatggg caaatttttg tgcgtcaagg agtaaccgat ttcgatagat aaaacttgag
     9961 ataccatatt cttctgtttc aggaatatga ttttgatagc caagctctaa ctctttgaac
    10021 cagcccactg aataggaagt ctcatctata ttaaaaagat ttgtatttag aattttcgat
    10081 aagtctactt cacctttaat tgtcctaatt attttggcat tggactgtaa cttagcaatt
    10141 agaccgttaa ttttgtaaat cgtcttggtt gacactaagt cactattatt taaaagtaat
    10201 atgttacaac actcgaattg atcaattaac aagccacgaa tatcctgttc ttcttcaact
    10261 acattacctt cagcttttct ttcctgaatc ttttcacccg tttcgaaatt atcccaaaaa
    10321 cgttttacat ccactactat aataatggca ccaatctcta atcgttgggt caaatcaatg
    10381 tctaaagggt ttttagctaa tataattgct tgaataattg gaattggttt gctgattcct
    10441 gaagcttcaa taacaatttg attcaagtca gaaaattgca ctaattgatc tagttcaaca
    10501 atcaaatctt cgcgtaatgt acaacaaatg catccattcg gcatcgaaac aagtttttct
    10561 ccagttcttt taaaaccttt cttgtcgact aataccccat caacatttac ttcccctatg
    10621 tcatttacaa tgacagcaat ttcctttccg tgatcaccgt acagaatatg atttagaagt
    10681 atcgtttttc ctgaaccaag atagccagtt aagattgtaa taggtatttt tttcataggg
    10741 aacctctttc tataccttga atttacactt taagtgcaac acataaaaat gagtcataat
    10801 cgatatccaa ctagtataag tttgtcgaga acactcctag aaggaaaata attatgagcc
    10861 agtatcgtca tcttactata ctcgaacgag aaagaatctt tctgcttcac gaagaaggat
    10921 tttctattag tataattggc aacgacattc agcatagccc gtttactatt ttacatgaac
    10981 tgagaagaaa caagaaaaaa actattcacc ttccaaagcg gaaaaaaatt atgcttatcg
    11041 aagaaaaaaa tgcggacgtt atcacattct aaatgatccg tgagtttggt caattgttcg
    11101 tcatttattt gtcgattttc agtggtcgcc agaggaaatc tcgaatcgtt tgaagcatga
    11161 acatatggac ttttcaatta gttttatcac gatctaacgt gcaatttgca atgatctttt
    11221 cgatacagaa cccctctcac acggcaataa aggcctgatc cgttcattgc accatcgagg
    11281 aaaaacaaga catacaaaaa atcatgtcga gtctcgtgga aagatttgcc ttactcattc
    11341 gatccatggc cgtccaaaat ccgcagatga tagaagtgat tttggtcatt ggaaagccga
    11401 tattgtagct ggcaaaactg gtggtacttg tttagtgact ttgacagaca gaaaatcatg
    11461 cttcctttta gcaggaaaga aggtccccag aaagaaagcc gcttatgtga gcgagaaaat
    11521 gattgaactt ttaagcagta ttccaaatga ggcggtcaaa acgatcactc cagatagagg
    11581 aaaagaattc tccaatcatt tcgcagttac agaagcattg aataatatgc ccttttattt
    11641 tccagatcct catgcccttg gcaacaatga acaaattaaa atacgaatag actcattcga
    11701 gaatacttgc caaaatcgaa agagatagct acggtagata actcaacgat aaatcagatg
    11761 attgaaaaac tgaataacag acctagaaag tgtttagatt ggagaacacc ttatgaagtc
    11821 ttctttgata aagtgttgcg cttgatttga caattcaaga cataataaaa agcagatcaa
    11881 atccaaataa gggaaaaatg atctactctg gtattatcaa ttaattggcc aagctattat
    11941 gccgttttgc taaacgtcta cttcttccag tattgacctt gataaaacaa caatcaggat
    12001 cccgttttaa tactctatga actgtgtcaa tactaacatg taaagttgca gcaatatcag
    12061 ctaatgtact cccatttaat ctcatttgct ttgcaaatcg ataacgccga taagcatcaa
    12121 tattcttgga atccatagac aataatactt ttcttaccat gtatttcctc caaaaattga
    12181 attttaatga gccatcattt cttgagcaat ttcatgttta gacaatgaag ctggatagaa
    12241 agtaggccaa ttatgcaatt cattatagag tttttcttga ctttctccac caaagaaaat
    12301 atggaaatgc tctgccttac ttggtgcaat tccatgatca ctaaattgaa tatatttaaa
    12361 acgaccagca cctgcatcat cagtttcaaa attaaaacga acaccacggt ttcctttttc
    12421 ataattcaaa attttgtatc caacatattt gtatgtgtat tgatgatgtt caccgttgac
    12481 tacaaaatca atggtgttgt ctttaatgtt gatagtatca atatctgtct tatagccagt
    12541 agtataataa tctttgtact cggcagctgt catgtcttta ttgatttttg ctttgtaatc
    12601 gaaaacttca tccaaaattc catcttttaa caacggataa acagattgcc attcgcctgt
    12661 ataatcactc aattcgcgat ccttcacatc ggcatcttta aagtacccat tagcaactgt
    12721 cttttcatcc ttcgcttctt tttctggaac tacctcttca ccagcaacca tagttgtttt
    12781 ttctaacgct tttagattgt cttccattac tgaaacataa tcttcaccat tatccatttg
    12841 ttcttttgta agactttcaa gaggattcag tacttccaat tttatcccag cttcgttggc
    12901 caaagttttt gcaattttat cattagcatt cttttcaaag taaatataat tgatcttatt
    12961 ttttttaaca tactctttta attcagctaa tcgaccagaa gatggctctt cttcaggact
    13021 tagtccagca ataggaactt gtttcaatcc ataatccaaa gctaaatatc caaaggctgc
    13081 gtgttgcgtc acaaaacttt tttgtttcgc ttctttaaga ctagtggtat agtcagcatc
    13141 caaccgtttc agtttagtca aatacttttc agcatttgtc tcaaatactt ttgctttttt
    13201 cggatacaat ttaactaact gatccttgat atttgaaact tccttaattg ccattttggg
    13261 tgaaacccac gtatgaggat ctagttcatg atggtgatct tcttctccgt gatcatgatc
    13321 ttcttcttca ctacctggca ataaaatcat gtcttttgtt ccttctacta cattaggccc
    13381 agctttcttc caactttctt tagcttttgg tacccatgat tccatgtttt cgttatgata
    13441 tacaaaaaca tcagcatcat gagcttttgc catatcctta gctgagggct catagtcatg
    13501 aggttctgat cccgctggaa ccattaaatc cacgttcgct tcatcgccga cgatgttttt
    13561 ggtgaaatca tacattggat agaatgttgt tacaatttct aattttccat ctttatcatt
    13621 cgatactttt gcatcattag tgctggagca agctcccaat aatcctagag ctgctatccc
    13681 aataagtcct acactgattt tcttcattct ttttccttcc ttaattatgc taatttcttc
    13741 attttatttc gtttatgttc tttgataatt tttaatgccc tttttctagt tttaatattc
    13801 gggcttttaa gtcgtcgata tgcactagat atatccgttt tctccattag aaattacccc
    13861 caatcactac ttatcattat ttgctttccg ccagtaattt atcaaaactc tgtttgatcc
    13921 tttcttcatc caacattttt ccgattagaa taatagtggt ttggtctttt tgtcgagtgg
    13981 tgtattgaaa attaacctgt tcgtttacct cttgcatggc aactataaaa tcctgctctt
    14041 gtaaagcaat caatccttta aaacgataca ggctcccttg gttggttaaa atcagccagt
    14101 caatccattg gagtaaaaaa tcttttttta tgtcttgatc tgaagttaag agaattgatt
    14161 taaactcatg tacttgatgg tgttgatgat agtatttgcc attatagtga ttttctgatg
    14221 aatcactttc aaaaactaag ggatgattaa attttttcaa attataaaaa tccttttttt
    14281 gaatcgtttc actatgtgtg aacattcgaa tatcggataa agggttaata cttttgattt
    14341 ctctctgtaa tttttttagt gcagataaat tcaagttttg ttttctagac acaaaaatgc
    14401 gatctgccat gacaagttgc ttaagggctt gtggttctct ttcaataagt tcaaaactgt
    14461 cactatcaat aacagtcaat aaactatcga tatatactct tcctcttagt tgaggcgctg
    14521 ttaaaagtgt ttgaatgatt ggttggggat ctgctatacc tgtagtttca atgatgattt
    14581 gtgaaattgg ttgttgatgt tcttctattg tttccagtaa agaaatcaat attgctaata
    14641 aatcactgcg aagactgcaa cacatacatc cattatttag ttgtaacacc cgttcctcgc
    14701 tatgaactaa taatttgtga tcaatcccaa cttcaccaaa ctcattttca attataataa
    14761 tttcttccct ggggtagggt gagacttgca aggcttgatt gattagcgtt gtttttccag
    14821 cccctaaaaa cccagaaacg attgtaactg gtatgcccat tttgtcacct ttttaccact
    14881 tagctatttc acttctttaa aaattgcccg tctgcgcaat tttggcgaat acttttttaa
    14941 ttctaaccgt tcagggttat tccgtttatt tttgctggtg agatacagac gttctccagt
    15001 ttcgacacat tccaaaataa tattttgacg cataaatctt cctccttcaa tacttatcga
    15061 tcttcaagat atgcttgaat ttgttgtgca tcttcaaaag ttaactcgcc agattccaat
    15121 tgtgaaacta aattttcttt catatcaaca ggtatctccc aataaccatc gatacattga
    15181 aataactcct cgtcatactc ttcaaaaatt ggatagattc ttaaaaaatc atcaaatgat
    15241 aagccttggt cctcatagta atctttgact aatccatttt caattgcctg ttgggtcggt
    15301 aaacccgttt cagtaataat tcctctctct accatcgaac gataaaaagt gcatttatct
    15361 agcgattgct gggtcgtttc aagataatac tttaatcctt cttcaactaa aggtgggtaa
    15421 ataatttcca ttttgattcc ctcctaaaag ttatatatta aactattttg aagctccttt
    15481 aattactttc tttccattat agtacccttt taaagataca tgatggctac gtctataatc
    15541 accattcgct tcgtcaaatg aaatttctgg ttttactaca gtttgatgag ttcttcttaa
    15601 ccgtttcttc gccttagatg tttttcttgc tggtactgcc atgatatttc ctccttttac
    15661 caacttgctt tttttactcc aggaatcaac ccttgatggg caagttctct aaacttaata
    15721 cgggacatac cgaactttcg catataccct cttggacgac catccgtctg atcacgaagc
    15781 ctcagacgat tcggatttga atctcttggc aatttagcga gagcggtgcg atctcctgcg
    15841 gcttttaatt cctgacgttt atctgcatac ttttcaatca tcgccatttg tttctttgct
    15901 ttagcaattt tcgacttttt tgccatatac ctttatactc cttttatttt gatttagaca
    15961 cctcaaatcg taatcgttac gttttgacac tgatacttta ccaacaagag ttgctcttgt
    16021 caacaataaa taacatgaaa taaaactcat atgaatagat aactatttga aaacgaattt
    16081 atcggtacat agagataatc aaatcattta aaagaatatg cagtttaggc tctgaaaaat
    16141 aagaattcac ttttattagt ccaatcaaac acaaataccg ttttaagtag cttttcctct
    16201 acttaaaacg gcaatatgaa agaaactata actttttatt caatctttta tgtttaactg
    16261 gtttatcgcc aattttttaa aactttgatt attccttata ttataagaat agacattaag
    16321 tgaactaatt caatcatact gattctttcg tgtattcaaa attagatgct gtttaataaa
    16381 attattcttt ttgaaaaaga attataacta tatcccaaaa attgtaggtt aatttaaagc
    16441 ctatttcatc atcgtgattg agcttctttt tcatagacga atttctcaga tagtttagtt
    16501 ttctttatga gatttttgta tatttctgac cttcgataca actcattatg agtaccttga
    16561 tcaactagtt ttccttgatc catcactaca attttattta ccttttcaat agacttcaag
    16621 cgatgcgata taattagtac tgtcttatct tgtattaaac gatttaaact ttcctgaata
    16681 cgtttttcat tttccgcatc taaactggca gtaatttcat ctaaaattaa aattggcgca
    16741 tttttcaaaa aagcccgtgc tatggatagg cgttgtcgct ctcctccaga aagtgaactt
    16801 ccgttttctc cgaccaatgt atgataaccg tcaggtagtt tttcaataaa gtcttcgcag
    16861 ttagctaaac gagctgcttt tttcacttcc tcatctgacg cttgactatt tccaatacgt
    16921 atattttcca aaatacttgt attaaacaaa gttacctctt gaaaaacaat agcgattttg
    16981 ctaaacaaag aagctggaga tacacgttta atatcatacc catcaattaa aatacagcct
    17041 tcatcataat catacagtct agacactaat ttcaagatac tagttttccc ggaaccacta
    17101 gcacctacta acgcagtcac ttctccttgt ttagccgtaa aagagatatg atctaaaatt
    17161 ggggtattgt tatcatatga gaaagagaca tctcgcaact caatgtcaaa agatttcaga
    17221 ggactatctg atccctcttg gatactggtt tccttcatag ctctaattcg ctgtatttta
    17281 ggagctaaat aaaatatttc aagcactgct tctttcatag aatcgaagga atcctttatt
    17341 tttattgcgg ctagtaagta accaacgacg tagagtatag tcacttcacc cgttaacagt
    17401 aagtgaacac ctactagaat gactactgct aaactaacat atgagaaaat agaggataag
    17461 gccatgactg aaaaagtact taattctacc tttaaatgaa tcctttcact ctcttccatt
    17521 tttttgaaaa gtctgtcttg aactttttta gatagattaa agctattaat ctcttgctgc
    17581 aattctatag tttcttgaaa ttcttccgag ttttctctca aagtatcgta atatttagta
    17641 ttggctttcg tttgggattt ctttgataac aagattaaga caaaactaaa taacgttggc
    17701 aaaataacag ctaatcccat tttgacatta ccaaccaaaa gcatcactga aataaaaggg
    17761 aaaaacagag ccataccgcc ggctttaggt attgcatgac tcatcgcatg ctcaatacct
    17821 tcaacgtcag acatgatagt ttgagataaa tctgataaat tatgttttga aaaataggat
    17881 aatggtaaat ttgacaaatc gtctgcaatt tgcactctta aatgcgcact ttctttataa
    17941 gtactattat ataagttttc atactcccta tctaacaacc aatacatgaa aagcaaagta
    18001 acaaacgata cggcaaaata aagccaacgg ggctttgtat tttctaaaac taattcgtct
    18061 atcagcatca ttataatgaa tgctggtacc atattaatgc agtatacaaa aaaactagaa
    18121 atagaagctt tagttaaagc ttctgcgccc tgatcactta aagcaaattt gtttttataa
    18181 aaacttttca ttgtcaagcc tccaatcatt tgcattctca taattagtga ccaattgttt
    18241 atacacttga tgtttagcca ttaattcatt atgatttcca cgttctacta ttttcccttc
    18301 tgacaagaca agaatttcat ctacagattt aatgctggat aatcgatgag ctatcataat
    18361 aactgttttt tctttcatca gatgttgaaa agccttttgt agttcatact cattatctgg
    18421 atcaatagat gcactggctt catccataat aacaatcttt gcatctttta aaattgctct
    18481 ggcaatagct atacgttgtt tttctcctcc tgacagataa actcctttac taccaattaa
    18541 ggtatgctct ctttcgggaa atttttctaa aatcgattcg caaccggcta atcttagcgc
    18601 gtcaaatact ttacttgctg gcgcttgagg atttgctata gaaacgttat cgtaaatact
    18661 ctgattgaat agtttcgtat cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt
    18721 tgaatagtga tttaaaggct taccaccaat tttaatctgt cccttatcca cattgtagaa
    18781 acctgaaatt agtttagcta tcgtactctt tccgcttcct gattttccta ctaatgcata
    18841 aattcttttt tgttctaaaa ctaagttgaa attttctaaa acacactctt ctttgtatga
    18901 aaagctgaca ttttcaaatg caatgtcaca atttttaatc tgtgtctcgt tcccatattt
    18961 tagttggtct tgcttcattt ctaaataaag ggagtctagt gtttcgactg catatcttgc
    19021 ttcaaacaag tgcatactta catacattat tctcatgaaa gaaacaaaaa taattccact
    19081 taaaaaaaac aacatcaaca agtcaacagc taatatcttg ggagtggtaa gagaagttat
    19141 aaaaaaggtt ataggaacta tcaaaatgca aactatgcca aaaaatagcc attgaaaaag
    19201 aacaaaaggt gttttcccac tcaaagaata cttatatgca aacttagcat aatcgttaat
    19261 tgctttattc aaggctttaa atgatgttac tttagcacca aatatcttta taacttgaat
    19321 tccgcggata tattctaccg tttcagagct taacacatct aacgattctt gatatttttg
    19381 aataaaattt gtattctctg tcattttctt taacaagaat acaccaatta ctaacatggc
    19441 gattagaacc aatccaattt taaagttaat gaaaaagcct aaggcaactg ataaaacagg
    19501 tgttactatg gcttgagcac tatctggaat caagtgagca attgccatat gtgttttagc
    19561 cgcgttgtca tcaattgtct ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa
    19621 acttgcttct gttaaaccgc taattccctt ttttctcaaa ttagtctcta agcgaaaccc
    19681 aaggatatga gaaaataagc cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc
    19741 aacagtttga accgcataac tcttagctaa atattcatct cctacaataa ttagagactg
    19801 aagaaatttg taaatactgt aatatccata gactgttaat acgacagaaa aatcagaaaa
    19861 taaagtggct aaccatccgt agtatttcat tttagggaca taactaaata attttttgta
    19921 tactttcatt ttttcctccc ccgcattcaa tttcttctct tattcgttta caactgttaa
    19981 tgctttccgt aatagcgtaa attagagatg tacaaacaga taaaaggtaa actacgttct
    20041 attttttgta tttttttaag acttttcttc caatcaatac accaatcata gataaacttg
    20101 ctacaactaa cactgaaatg attccccata tacctaccgc atagtggatc attgcatcca
    20161 ttgttgcagc agattgtcct ttatctatcc aatcttgacg aaaacgatct gtaaagaacc
    20221 atataggaat ccactgtccg gcagcactgc ctaattgcat tagagcccaa gacgcaactt
    20281 gaggaattgc tcgatcatat ccaaatacag atagaataat atcagctaag atcgccatac
    20341 taatcgaact gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa aataacaata
    20401 aaacaattgt gtaaatagta aaaattcctt ttctagggta gttatagcta atataaacaa
    20461 atatgattcc agataataat ccaaaaatcc cgggggatat gctgtgacca aacgccccac
    20521 caatactact aaccattatt gaaaaaaagt aaattactag ggaaataact gtagctaaac
    20581 caataaaaat ataatctttt gttttcatat atattctcct caattctatt tcgttatgtc
    20641 attaaaaatc tgttctatca aagttgaatc ttctactcta agttttatat ctttttttat
    20701 tccaccattt tttaaataaa taattctatt gcatgtttta taaagaaatt ccggatcatg
    20761 agtagctata actactgcac ccttttcttt taagtcttta ataaggtcag caatcctttc
    20821 catattaata taatccaacc cacttgtagg ttcatcaaat agaaataaat tagtttttga
    20881 caatgaagct atagaaacca ataatctttg tttttctcca ccggataaat caaacggtaa
    20941 ttgatctttc attttataaa gttccatttt ttttaaaatt ttttcttgct gatctttact
    21001 aactatttca ttccccaggg ctaactcgct cctcactgat tctgtgaaaa attggtaatc
    21061 catttcttgc attactaaaa acggcgatga caaatttttt ttattttcga ttatctttcc
    21121 tttggtaggg cgaataatct tcatgagtat tcttagtaag gttgttttcc ccacaccgtt
    21181 tttccctacc aaaccaataa catctccaac tttgacatca aaatttatgt cttttagaat
    21241 attttcataa gtaatttctt caacttttaa tacatctgca cttacctttt cgatataggg
    21301 aacttcaatc gaactattaa aaatgtcaaa tttccttaat gcgttattgt tttcagtttt
    21361 taacaactga atagttgtag tttttacagt tctattttta acaaatagaa cttgatcaat
    21421 taattctttc aaatagtaaa aacgatgttc agctataaca atagtaaatc ctttattttt
    21481 taaatcacaa agtaagtcag caagtttctt agtcgctttg aagtccaaat ttgaagacgg
    21541 ttcatccaat ataattaatt tagagtttag cgataaacta catccaatag aaattttctg
    21601 ccgttcacca cttgatagcg aaaaaatgtt tttatctaat atatttgaaa gatctaataa
    21661 ttcagctaat tcattgattc ttttatccat atgctctttt gtaaatccaa agttttccat
    21721 cgaaaatact aactcactcg ttgtatttgt tgtaaagaat tgtcctctag gatcttgact
    21781 tacgtagcca atattttttg atatactttc ttgtgttaag gattgtagcg agttattttc
    21841 aaataaaatt tccccaacca attttccgtc ataaagtctt ggaataagtc cattcattaa
    21901 ctttaatagt gtggatttcc cactcccaga agaaccagta atcaatgtaa gactgccttt
    21961 ttttattgaa aaattaaggt caacaagcgc ttgttcctta acatccttat atgaaaaaga
    22021 aacattgtta aattcaaaca attctttcac ctcaaatcaa aatacttaag aaaaaaatag
    22081 taacggaaaa caggatgata tagtctaata agctcatgtt tgttttatag taagaacttc
    22141 ttttcacagg agattctatc ccctttgtta ttgcattagc ggtcatttct tcagcaatca
    22201 tagttgcacg tataagaacg ggaactaaca gaaattccaa atattgcaga ggctgaaaaa
    22261 aagacaagcg aatgtttctc atttttaaag attctttaat cattcttatt tcagaacgat
    22321 aagtcggaaa gaatctgatc actactgtaa tacttaacat tatttttttc ggtaaatgta
    22381 ttttttctaa agaggataat aattcattgc tagtatagac actaaccaac aaccatgaga
    22441 tcattacgac cggcaacatt cgaaatacaa tatataagga tgtcgcaaaa aatatagtaa
    22501 aaaactctat cttgatagca tcaataattt tcattactaa aaacagaact gaataaatca
    22561 agatgaaata aatacatgtc tttattttac gaaacatcaa tactaaaact gtagcaataa
    22621 tgaaacaccc cacttgaaat atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa
    22681 tatttaaagt aatcaaggtt aacggattga tttttgacac tctttcacct ccaaaaaata
    22741 agatcgcact gttatcttaa gctgataaaa atgcaccgtt attcagataa cagtgcgatc
    22801 ttatcaaaaa gcataactga gaacaattct caatgcaagc tttttttaat taactcagta
    22861 attcaatcca cccaggaaca gaaaattgat acaatcttct ggttaatagc atagtctttt
    22921 cttttgaata gctatgcttc aatatatcat agaatgtagc gtagaatgag taaatgtaga
    22981 agtggatttc ttcttcatta aagactaaat tttgattgat cattccgata gtctttaact
    23041 tatctagaca acagactgtc tttttcgtca caaatgatgt aattttttca acaatatctt
    23101 gatatttggt tccagctgat tggaataaaa tcaaatcgaa tatacgttta ttctcaaaaa
    23161 tataattaat aaatggatct aattcattga agttctctct ccaacttttc tctatatcta
    23221 tattttctaa gtttaagagg tatttggtaa actcttcatt atacatatta ataagcgcat
    23281 taatatgcgg ctgaataatt tcttcaaata acgcttcctt agaagtaaaa tgtttataaa
    23341 aagcacccgt agttacgcct gcggtcaggc acagttgcct tatcttagtt tgctgaaatc
    23401 cttctttttc aaaatggctt agtccacttt ctattaactt ttggtgagtt ataatataat
    23461 ctctggtttc tatgggtact tcctcctaat aagatgaatt aactttatta tcttacacag
    23521 gacttgaagt ttcaatcgtt aacgtttatt actgaactca ttccttagag ctaaactaat
    23581 gagaaagagc agaataactg atacaaaaag caatcctaca agaattaata gactcatcta
    23641 aatcattcac cttttctaaa ataatccgag aaagatttaa aaaaattata tccagctatt
    23701 aggataagtc ctatcataat cactccaata gccattttct tatcctccct aaaaataaag
    23761 tcttttactt aaaacctttt ttgtaatcgt caataaattt tttcccttta tcagttaatt
    23821 gaaatcctat atctaaatag tcctcatatc taagtttagc cagcatctca cttacagatg
    23881 ctggactaac acttagaact tttgctaaat cattattttt taatttcttt tttctttttg
    23941 ataattcctg aattgctagt aaataaatta gtatagtagt cgttttaatt tgtaccatac
    24001 ctaccctcca aattatttcg aaaggccttc agcaatttta tccaggttcc atttcatcat
    24061 cgcatagtaa ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa aaatcgttga
    24121 atagatagga atattggtat cttttgatac tgttttcatt ggtctatcat ccacactgct
    24181 ttctacgaat aaggagggaa cttttgttgt gcgtaatttt tcaactaagt gttttatttg
    24241 atctggtgtt ccttcttctt cagtgttgat ttcccaaatg taagcagaag gcacattata
    24301 cgctttcgag aaatatttaa agcatccttc acttgtaaca atcatttttt tatcattcgg
    24361 aattgaagca aatttagatt tagcttcttt gtctagagaa tccaattttt caatatactt
    24421 atctagattt tctttataga attttttatt atcaggatct ttttccgcta actgtttttc
    24481 aatattttta gcgtaaataa taccgttttc taaatttaac caagcatgag gatcttcctt
    24541 ccctttctca ctctgaccct ctaagtaaat aacatctatg ccatcacttg ctgcaaaata
    24601 gtctttgttt tcctctttgt tcgcattttt aactaatttt gtaaaccaag catttcctcc
    24661 agtttccaag ttaacaccgt tataaaaaat caaatctgct tttgaagttt tttgaacatc
    24721 ttcaggcaaa ggttcatatt cgtggggatc tttcccaata ggtacgatac tgtgtaaatc
    24781 aattttatct tttgctatat tttcggtaat atctgctaaa atcgaattag taactactac
    24841 ttttaatttt tcgttcgaag ccactttatc tttgtcttta ctatttattg aacatgcccc
    24901 gaatataaaa agagcaatca aaatccccag acttcccagt actgctttcc attttctcat
    24961 cataacgatc tctccttctt tcttaaaaat ttttgttttg gtgagattag aaaactaatt
    25021 acaaataaga atgccgctgt taaaacaata cttgatcctg ctgctatgtt aaaactataa
    25081 ccaataaaca atccaagcaa tgatgaactt gctccgactg ttgcagaaat aaatatcata
    25141 gttttcaagc tatttgcata taaatatgct gtagctgctg gtgtaattaa cattgcaaca
    25201 attaatatag ttcccacact ttgcatagct gttactgcta ctaaagttaa tagaaccatc
    25261 aatagataat gataaaaact gacattcatc cctattgctt ttgccattaa tggatcaaaa
    25321 gaagtgatta ataattcttt aaaaaataga actactataa ccaacactaa aaccgaaacg
    25381 ccaattgtaa tccacttatc aatatcttgt acagctaaaa tatttccaaa taaaatatga
    25441 aataaatccg ttgagctatt tgcaactcct ataaggataa ctcctagtgc taaaaaagaa
    25501 ctgaaagtaa taccaattgc tgtatctcct tttattacac tattgctttt tataaaagta
    25561 ataatgatag aagctaatag accaaataca atcgcaccaa taaaaaaatt aatacctaaa
    25621 atataagaaa ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc cataagagac
    25681 attcctctta aaataataaa acaacctact gttccagaaa caatccctat aactacagat
    25741 gtaattagag cattctgtaa aaagtgaaag tcatacagac cattaataaa attttgaagc
    25801 atattattcc ccctctccaa taaatatagt atctccatag gcttttttta aattttcttc
    25861 attaaataca gattctacac ttccgtgagc aattagattt cgattcaaaa gaataatatt
    25921 atcaaaatac ttctttacct tgcttaaatc atgatgcaca attagcactg tttttccctg
    25981 atgtttcaat ttttttaacg tatccataat tattcgttca ctaactaagt ctatccctac
    26041 aaatggttca tctaaaaata tgaaatccgc attttgagca agacatcttg ctaaaagtac
    26101 acgttgaaat tgtcctccag ataattctcc tatttgatga ttagaatatt ttcccatatc
    26161 gaccttttcc aaagcctttg aaactctttg ccattctgca tttttaattc tttgaaaaac
    26221 tttcattccg gcatatgttc ccatagatac acattctttt acttttattg gaaaagtaaa
    26281 atcaatatca gctttttgct ctacatatgc aatcttcacc agttttttat tgatatcttc
    26341 attattaatg gagacctctc cctgatgagg gataatattt aacattgctt taagcaatgt
    26401 ggattttcca gcaccatttg gaccaataat acctgttaaa gcaccttctt gtatttttaa
    26461 agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca ttaagctttt tcacttctaa
    26521 cataactttc tccttctaaa tgataatgat tatcacgatc atatggtata caaaaattat
    26581 attttagtca accctaaatt ttatagttta gatttttttc tgttgctgaa gattagaatg
    26641 acaaaactat ttgaaactac gcgtatagta agtagctttc aaaatatata aaaaaggagc
    26701 gttctcaaat gagaacatgc tcctaaaact actttattgg cgtatggaaa aattatactt
    26761 gaagtggata gcgggttgaa taagttgata agcatcaaaa catttataaa aattattcaa
    26821 gactatgtct gttccatata caaatatgaa aatcagtaaa ctccatttaa cttttttcgt
    26881 agtgtgtctt tttatacatg tttatcgtta ttaatagtga ataggaaaaa aactattttt
    26941 gatcattgac aattaaatac aatttttgaa taacgatccc tgctattcga gctagaaact
    27001 caagtacgcc atgacctgat tagcgacaaa tacttagaga tttctttttg ttctgggaaa
    27061 acaaaatgcc atgacaatag cagcaaataa tgatacttcc gtaaggctcg ccgtaaactg
    27121 gggagaggtg aaattcctat gtgcttaccc aaagcaattc aaaaatttca taactactat
    27181 tttaggagat gattttatag aatccagagt ctacctgaat aaaaaacaaa cctgtgcatt
    27241 tctaggaatc gataataata ctctagataa gtggattcgt atatataatt tcccagtaat
    27301 aagagttggt ggtgtagttc gctttgacgt agaagatatt aaagacttta tgaatacatt
    27361 taaaataact tgaaattttc ttctcgccgc atatactaaa attagtgaaa tatactgcct
    27421 tacttttttt aaggagtgag gattttgcct acaaagcttt caaatgggaa atataaaact
    27481 aatctgagat atccaaaaag gtttaaagaa ataacaggaa ttgcatcaga aaagtatcaa
    27541 aaaacatttc caaataggca attggcgatt aaagccgaaa atgatatgaa gaagaaaata
    27601 gaaaaagttt tgagagagga gaatgcaaac tcattagaat taaaaggaaa aataacgttt
    27661 aaaaaatttt acgaatctaa atggcttcct agatatgaat taggtcaaac aattagatca
    27721 aacaggcctc ctagtgatat aaccattagt aatacaaaag atatatttcg gttgcatatt
    27781 ttacctatgt ttggtgaata cgcaatgaat tatctaaata tcaatactga gataatatca
    27841 gatgaattaa ccaaaaaatc gaaagaatat gcaaatataa aaatcataaa aggatatgtt
    27901 cgttccatgt tcgatattgc agaaatacta aattatatcg aatttaatcg aactactaaa
    27961 attattcaaa gtatcactgc acctaaaaaa aatgctttag aagaaaaaag aatacaagaa
    28021 ggtaagcaag cactatcatc aaaagaactt actaattgga tagaagcagt aaatgatgat
    28081 tttaataatc atttacttac ttttcatgat tacacacttt ttatgattac cctgtatctt
    28141 ggtgatagaa aaagtgagac ttacgctttg caatggaaat atattgattt tgaaaaacaa
    28201 acggtccgtc ttaaacatac tttagataaa tatcaaagaa aaaagtttac aaaaggtcga
    28261 aaagataccg tcattcaagt accagaagtt gtaatgacat tgctaagtga atggaaatcg
    28321 gtacaagctg atcaattgtt gaaattaaaa attaagcaaa cgttagatca gtatctcttt
    28381 acttatacta aaccatctgg agaagtaaat tgtcctgttc atgccgatta cctaaactat
    28441 cgaatcaatt caattaaaag aaggcatcct gacttagttc atttatctcc acacaaactc
    28501 cgtcatactt atgcaactat agctagacaa ggtggagcag atatgaatca aatttcaaat
    28561 gctttaacac actcagatat ttctacaaca aagatatatg tgaacacacc agatatagtt
    28621 gataaggctg tttttgaagc ttttcagaga ggattgaata agtgtgattg atatgtgttc
    28681 ttaaatgtgt tcttaaaaac aaaagaagca ctttccaaat aattgaaaag tgccagaaac
    28741 gttgatatat aaaggatatt aacgttttga gaattgtgaa gctttacgtg ctttcttaag
    28801 acctggtttt ttacgttcga ccatacgtgc gtcacgagta agtaagccag cacgtttaag
    28861 agctaaacgg aagtcaggat caacttctag caatgcacga gcgataccat gacggatagc
    28921 tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat atgcgccttt
    28981 tgtttcagta acagcgaaag gttgattgat tacttcacgc aagtcagcat gtggaatgta
    29041 ttcttcaaca tcttttttat ttacagtaat tttgccagtt cctggtacta gacgtacgcg
    29101 agcaactgcg tttttacgac ggcctgtgcc gatatattga acttgtgcca atgaaattcc
    29161 ctcctattag attaagtttg tgatgtctaa aacttctggt tgttgtgctg catgtggatg
    29221 ttctgctcca ccgtatacgt tcaattttgt aaattgtttg cggcctaaag tgtttttagg
    29281 aagcatacct tttacagaag tttcgatcaa acgacgagaa tttttagcac gcaattcgcc
    29341 agcagtgatt gatttcaatc ctcctgggta gttgctgtga cggtaataaa ttttgtcagt
    29401 cgctttttta cctgttaatt tcactttgtc tgcattgatg acgatgacga aatcgccagt
    29461 atccaaatga ggtgtgaaag ttggtttatt tttaccacgt agtacagatg caacaactgt
    29521 tgaaagacgt cccaatggaa catcagttgc gtctactaca taccatttac gttctacttc
    29581 gcctggtttg gccatatatg ttgtacgcac gttattttcc tccaatttaa gttctgatgt
    29641 ttgacataca cttcgagttt ccggggctct ttgtggggca aacaatacca tttaacatat
    29701 tacccgctaa tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac
    29761 tttacttctt ttctttcttt tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa
    29821 agaggctgtg acaaaagtct tgtcacaacc ccttattttg aataaacggt gttctcctgc
    29881 caactcctcg gtcacaagtc acaatatcca taaaacataa gaagctgttt tttaaaattg
    29941 tttcttgtga ctcggagctg a
//
LOCUS       DACEDM010000037        29630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEDM010000037 DACEDM010000000
VERSION     DACEDM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.037"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..919
                     /locus_tag="IWO50_001449"
     CDS             <1..919
                     /locus_tag="IWO50_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0358578.1"
                     /translation="FSFFRSYRGDTFYGDLIVPTIIAIILIVKWTKFTVIDRSLYLVV
                     FYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSDIWINTNTIGSSLMTLALLITGFASS
                     FGKWYMRLLSLPALILAGATIWVCQSKAALYALVIFIILEILPKKVFRNTHLSFWMYA
                     AVSVLALPISYFAAMSDKVHLFTGREEIWRKFYETLGEKTGQIFVGMKPFFFHRGNQI
                     LGNHNTYHALVNHYGLIGFVLSIILLLSFLWLLVRKRELTNGQLTFLWAFLAIMCQSF
                     MEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            1001..1672
                     /locus_tag="IWO50_001450"
     CDS             1001..1672
                     /locus_tag="IWO50_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0358579.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAKMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1725..3701
                     /locus_tag="IWO50_001451"
     CDS             1725..3701
                     /locus_tag="IWO50_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0358580.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            3714..4466
                     /locus_tag="IWO50_001452"
     CDS             3714..4466
                     /locus_tag="IWO50_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0358581.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4482..5243
                     /locus_tag="IWO50_001453"
     CDS             4482..5243
                     /locus_tag="IWO50_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358582.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5256..5516
                     /locus_tag="IWO50_001454"
     CDS             5256..5516
                     /locus_tag="IWO50_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358583.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5513..7603
                     /locus_tag="IWO50_001455"
     CDS             5513..7603
                     /locus_tag="IWO50_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0358584.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(7639..9696)
                     /locus_tag="IWO50_001456"
     CDS             complement(7639..9696)
                     /locus_tag="IWO50_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0358585.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(9841..10032)
                     /locus_tag="IWO50_001457"
     CDS             complement(9841..10032)
                     /locus_tag="IWO50_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358586.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            10220..10675
                     /locus_tag="IWO50_001458"
     CDS             10220..10675
                     /locus_tag="IWO50_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358587.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            10672..10914
                     /locus_tag="IWO50_001459"
     CDS             10672..10914
                     /locus_tag="IWO50_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358588.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            10932..11234
                     /locus_tag="IWO50_001460"
     CDS             10932..11234
                     /locus_tag="IWO50_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358589.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            11303..13334
                     /locus_tag="IWO50_001461"
                     /pseudo
     CDS             11303..13334
                     /locus_tag="IWO50_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13334..15946
                     /locus_tag="IWO50_001462"
     CDS             13334..15946
                     /locus_tag="IWO50_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0358590.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            15943..18321
                     /locus_tag="IWO50_001463"
     CDS             15943..18321
                     /locus_tag="IWO50_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358591.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            18382..18924
                     /locus_tag="IWO50_001464"
     CDS             18382..18924
                     /locus_tag="IWO50_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358592.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            19002..19334
                     /locus_tag="IWO50_001465"
     CDS             19002..19334
                     /locus_tag="IWO50_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358593.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19335..19928
                     /locus_tag="IWO50_001466"
     CDS             19335..19928
                     /locus_tag="IWO50_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358594.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19950..21974
                     /locus_tag="IWO50_001467"
     CDS             19950..21974
                     /locus_tag="IWO50_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0358595.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            21974..23173
                     /locus_tag="IWO50_001468"
     CDS             21974..23173
                     /locus_tag="IWO50_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0358596.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            23189..23833
                     /locus_tag="IWO50_001469"
     CDS             23189..23833
                     /locus_tag="IWO50_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358597.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            23844..24650
                     /locus_tag="IWO50_001470"
     CDS             23844..24650
                     /locus_tag="IWO50_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358598.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            24673..24902
                     /locus_tag="IWO50_001471"
                     /pseudo
     CDS             24673..24902
                     /locus_tag="IWO50_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25061..25996
                     /locus_tag="IWO50_001472"
     CDS             25061..25996
                     /locus_tag="IWO50_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ0358599.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            26040..26309
                     /locus_tag="IWO50_001473"
     CDS             26040..26309
                     /locus_tag="IWO50_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358600.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            26586..26864
                     /locus_tag="IWO50_001474"
     CDS             26586..26864
                     /locus_tag="IWO50_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358601.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
     gene            26903..27325
                     /locus_tag="IWO50_001475"
     CDS             26903..27325
                     /locus_tag="IWO50_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358602.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            27345..28937
                     /locus_tag="IWO50_001476"
     CDS             27345..28937
                     /locus_tag="IWO50_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358603.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QTQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            28967..29302
                     /locus_tag="IWO50_001477"
     CDS             28967..29302
                     /locus_tag="IWO50_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358604.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            29443..>29630
                     /locus_tag="IWO50_001478"
     CDS             29443..>29630
                     /locus_tag="IWO50_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358605.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        10630 a         4630 c         6048 g         8322 t
ORIGIN      
        1 cttttctttt tttagaagct accgaggaga tactttctat ggtgatctga tcgttccgac
       61 aattattgct attattttga ttgtaaaatg gacgaaattt actgtaattg atcgtagctt
      121 gtatcttgta gtattctatg tctttttact cgtaacggtt tatcgagtat ttactgagat
      181 accagtacca gaaggacaaa gtatttggaa tccatcgaac aaattatcag atatttggat
      241 caatacaaat acgataggta gttctttaat gacattagcc cttcttatca caggatttgc
      301 tagttcattt ggaaaatggt acatgcgatt gctatcatta ccagcgttga ttcttgctgg
      361 ggcaacgatt tgggtttgcc aatcgaaagc agcattatat gcgcttgtta tcttcattat
      421 cttagaaatt ttacctaaga aggtttttcg aaatacacac ttgtcctttt ggatgtatgc
      481 agcagtttcg gttctagctt taccgatttc ttattttgct gcaatgtcag acaaagttca
      541 cttatttaca ggacgagaag aaatttggcg caaattctat gaaacattag gcgaaaaaac
      601 aggacaaata tttgtaggga tgaaaccttt ctttttccat agaggaaatc agattctagg
      661 taatcataat acgtatcacg cactcgtcaa tcactatgga ttgattggtt ttgttctatc
      721 gattattctt ttgcttagtt tcttatggtt gctcgtaaga aaacgtgagc tgacaaatgg
      781 acaactaaca ttcttgtggg cttttttagc gatcatgtgt caatcgttca tggaagaaac
      841 attaacgaca atcagttggg tgccaattgt attcttaatg ttagcaatgg ctacacacca
      901 atataaaacc accagataaa tgaaatagaa tagtaataag agaaaacact ttttcgttgt
      961 gaaaaagtgt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
     1021 ttttttagga gtcgctttaa tcgtcgtttg cagtgtgttt ggctatgtta gctaccaaaa
     1081 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
     1141 tgcacacgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
     1201 gcaaatgttg tccatacaaa aagaagcaat caaaatgggc gtcaataaac aagtatttgc
     1261 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
     1321 cactttgagt cttggagcgg ctacttattt ttatgaagat gccaaaatgg gcaaaggaaa
     1381 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1441 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1501 aacgcgtaaa tttatcgtac ctgagtactt taaattaatc gatggcgttc ctgaagagaa
     1561 ctctttcttg tctttaccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1621 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaagaatt
     1681 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1741 atgtaaaaaa agctggaatg attgttggaa tgttaggaat cattgcaggc tgctttcagg
     1801 ggtatcgagc ttatgcagaa gatgtcacgc aaaaaacgcc accagaaagg gtcaatatca
     1861 cggttcataa attaatgtac gatcaaagta cgcaattgaa cgttgatatt gacggaatca
     1921 aaaatgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1981 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgactgaag
     2041 attctgatct aaccaacgcg aaagtggacg aaatcgttaa agatgtagaa gacaagggat
     2101 ctaacagtga gtacgttaag aatgcaacca ataaaattac ttcagctgta gatgaaaatg
     2161 gtgagatcac ttttgctgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     2221 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     2281 ttgcgccaat gacagacaat acaggatcag gatttttaaa agatattcat ttgtatccaa
     2341 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcacagctc
     2401 aaagcaaaca aacaccttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2461 aaggaacaaa attaggagag cttgtatcgg atgatcaagg aaagctgact gcaactgatt
     2521 tgacgttagg aaaatactac tttgtggaag ttccttcaga agttgtagtg ggatcagatc
     2581 aagaaccaac agcagaccaa tatttgttag gcgcagacgc tcgcaacgat gcgcacaata
     2641 agttaacctt tgaaattacg aatgacggcg tgacaagtga tttaaaagct tcctacgtga
     2701 actataaagc accagtgatc gataaaacag tgacaaatgg aacaggagaa gaacattctt
     2761 tccaaattgg tgacgcagtg aattatcaag gtacaattca tatccctaca gatatcgcag
     2821 gtggaacaga tgggattaca gtaaatggtg taaaaagtga aacttctcct tacagtgtat
     2881 ttaaatgggg cgacacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2941 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     3001 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     3061 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     3121 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     3181 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     3241 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     3301 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     3361 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3421 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3481 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3541 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3601 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg cttggggcag
     3661 gcgcaatttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3721 tcagacaccc taagaaaaag cgaatcatgg gtaaatggat cattgctttc tggttacttt
     3781 cagctgttgg tgtcttactt ttgatgcctg ctgaagcgag cgtggcgaaa taccaacaaa
     3841 atcagcagat tgccgcaatc gatcgaacag gaacggcagc agaaacggat tcttctttgg
     3901 atgttgctaa gattgagtta ggcgatcctg ttggtattct tacaattcca agtatttctt
     3961 taaaactacc aatttatgac ggaacttccg acaaaatttt agagaatggt gtagggatta
     4021 cggaaggaac tggcgatatt acaggaggaa acggtaaaaa tcctttaatc gcagggcaca
     4081 gtggacttta taaagataat cttttcgatg atttgccttc agttaaaaag ggagaaaagt
     4141 tttatatcaa agttgatggt gagcagcacg cttatcaaat cgatcggatt gaggaagtgc
     4201 aaaaagacga actacaaaga aactttgtca cttacttaga accaaatccc aatgaagatc
     4261 gagtaacgct aatgacatgt acgcctaagg ggatcaacac acaccgtttc ctagtgtatg
     4321 ggaagcgagt aacctttaca aaatcagaat taaaagatga agaaaataag aaacaaaaat
     4381 tatcttggaa atggctttta ggaagtactg tttttctttc agtcatgatc attggttcgc
     4441 tatttgttta taaaaagaaa aaataaagga gggtaaccaa catgagaaat agaaaatggc
     4501 tatttgtttg tgcagccgct cttttttctt tcactgccta cacacagttg acgtttgcag
     4561 acagacaaga aagcacggat agcacagaac aaacaatcca aacgtcagat agcagcacga
     4621 ttcaaagcag cgatgaaacc gtcgattcgt caacagttga ttcttcagaa gaacctaaaa
     4681 ttgctgaaga cgatcaaata gtcaaacaga ccattcacgt tcaaaaaatt atttcttccg
     4741 aacaattaga caaccagggg caaaagcttg aaaaacaaga aggagtaaat ggcgcgaagt
     4801 tttctgttta tgatgtatcg gacattttac aaaagatgga tgttaaggat ctcactactg
     4861 accaaattga atcccaatta aaagatcgag tgaaaaaact atccagtgat caactgaagc
     4921 taatttctaa tggagaaaca aagacaattg atcaacaaac aggtgtcttt gaattctcgg
     4981 tagaagtaca ggcaaatcag aaacaagctt attatattgt caatgaatca agtcctgaaa
     5041 acatttctaa tagcgaggat attttgctat taacacctgt ttctgataaa aatggtgaat
     5101 tcttaaagga tgtgtggatt tatcctaaat cagaagcttc gcaacctaaa gaagaagtaa
     5161 aaaaaatcgt atctactggc gtaaagaaaa acttctttga gaattgttgg gattttgtta
     5221 ctcacttgtt ttctagagat taaggaagaa ataaaatgaa actgatcaaa ttattattat
     5281 tcaatttttc ttgtttaacc gcagtctttt tgtactgcgg tttattattt tctaaactat
     5341 ctattttgcc aagctaccaa ctatacctaa tgaggatttt aaacatacca gctgcttcca
     5401 tgccgctaga tattatggaa aagctcatta ggaatccatt atttactacg atcaatttga
     5461 tcgttattgt cggaatttca ctagtcattt ataaaaaaga ggggatgaag ggatgaattt
     5521 taaacactcg tggacaagac ggttaaaata tgtttttccc ataatgatga tgttaggtac
     5581 attgtttggg ttaaaaacag ccaatgtctt tgctgaaaaa gttattgtcg atccgtcaaa
     5641 tccaatccag aacgtaaaga ataattttat tattccaaaa gatgttccta atgcaaaaac
     5701 aaatattact gtaaacggag cgtcaaaagt ttacaaatat catgttgatg ccaacaaagg
     5761 tggttttaca gacgactacg taggattagt ggagggaaac aaaaatatac gctatccttc
     5821 tttccaaaaa gaatacggtg aaacaaacct tgttttagaa ggcgtaacaa ccaacaccaa
     5881 agtcaatatt gactatggaa aaatcggtac gtataacggg aaaaaagtca atatcaagct
     5941 agttctatct aatattcact tatactcgga tacgttgccg tggaatctac tagataacaa
     6001 ctatacaaaa actcattttc gagacgatgg ctataaaaat acgaatggtg caatgagtaa
     6061 atctaaaaaa agaacagtac tttggatctc agacaatctt ttctcaggaa ttgtttacca
     6121 ttcaacacaa atgaatgtgc agctagtagc cacatatgag gacggtagcc ccgttcaatt
     6181 ttcaggagat acatttatca gttttaactc tttgaaccct gcaggaggaa aaagcacgga
     6241 tcttaaagga gaatatgccc actatgacaa aatgaatacg acagattggt atgtcagaaa
     6301 agatacggtt ctttcagaat ttaaatcttt ctacaacaat ctgaacgtgg taggtggcca
     6361 cccaggagga agctctaagc tgacacaagc ggataatgat ttcaataatt tgcatgataa
     6421 attaggagat ccgaagtttg gtcaaggtac tgtgtccttt aaaatatctg aagcaaatcc
     6481 gacgtttgtg attggttctt ctaatgttca aacctggttt actttatctt ctgcgacaat
     6541 ctttagtgtt gttcctgatc aacctgaaaa aacaggggtt gataaaaatg gaaataacgt
     6601 gaacgataaa atgttgcaag taggagacac gattcaatat cgaatcaaac agaaggtgaa
     6661 tcgattagga gtggatttac tagcggttta cgatcgattt gagttaatcg ataatttacc
     6721 aaaagaagta aattacgtcg atgcacacgt tgaaagtggt acaaataaaa aattcgatgt
     6781 atctggtgaa gtgacctatg acaaaacaaa acaccaggta aaatatgcag cgaaagctga
     6841 tacattaaaa aatcgaatga agtataacgg cgaaacttac gaattagtga tcaatgtaaa
     6901 agtcaatgaa ttggccaatc aaaatagtgt agccaaaaat cagggaacgt cgatcattaa
     6961 taaagtagaa aaggatacaa atatcattac cgtttatttc cctaaaattc cagtgaaaga
     7021 agtacaacaa aatggaaaag atgtaaacgg acgaaatgac ggtaagaagg gtacgccgac
     7081 agctccttta aacgcaggat ctgaagtaca atatctagtc acacaaaaat ggcacacaaa
     7141 aggcgtagac gctgcgtcag atcactacaa acaatttagt attcaagatc caattgaagc
     7201 tcgtttgaca tataaagaag gttctgcgca agtaatagat aagagtaaag gaaaagatat
     7261 tacttctgaa ggcacactaa cctatgatag caattcaaga acgctgaagt gggaagcttc
     7321 cgctgacttc ttaaataaca atcttttaga tggacgagaa attcagctga tatttacagc
     7381 taaaactcca ttacaatcag aaaaaaatat tgataaccaa gccgtagtag cagtagataa
     7441 tgtttctaat aaaacaaatg ttgtgacaat tggggttgat cctaacttgc cacaagttat
     7501 tgttcctaaa acaggttcta cacatttagt aacgatttta gcggtcagct tactattact
     7561 tgtgttagca acttttggct acgcagttct gaagttcaat taaacaaaaa aacaaggctg
     7621 aaaatcagcc ttgtttttct atttgaaaat gggattggca ctaataaata cgttgtctga
     7681 agaaagtgat ccgtctgcag aaacttcata agcatgggat aaaccaacga tactttgatt
     7741 gttgtaaagc aagtttgcgt tatgagcagc gctatctttc cacataacca tagcatttac
     7801 tgctcctgta tctgaaagga tctcgtcaac gtaaaggtag aaactattga tattttctcc
     7861 tttagcaccg tagcagccta agctttctaa gagatcttgg aaagtaccgc cgccaggtcg
     7921 gtaatgactg aagcttactt gtaattcttt tgcacgaata tctgcagctt gttgaacgat
     7981 atcattgaat tgcattgggg caacgccatt ttgttgacgg tagctattta accaagcaag
     8041 ttctttttga cgcacatagt tgtttaaatc acttagtgag atatttttgt atttgaaagt
     8101 gatcgttcga ttttggtcaa cattattgat tacacgttgt ccatttttat caacggtaac
     8161 agatgaatcg tcatttctac acaagaagta gttttgatcg gtcatatttt tccaagaagt
     8221 agagaaacta tctccctcgt aaaccgtttg tgtttcagta gataaaacag cgtttgtgtc
     8281 aacgtttata tggttcacag tgacgttgta ttgatgagta taggcaaaat tgattccttg
     8341 attacttgta acgttgttta aagtcacagt gttgctagag ctttctctag gatcaagaac
     8401 aaaattagtg aacataggtg cttcaattgt gatgctttgt ccataaacga cgggataact
     8461 cttactgtaa ttatagagtt cttggatatt tggatcagta tccgagactt tatttggacg
     8521 agctttcact tcaactagaa agacttcatc atagtaaaaa gtaaatgtgg tatttgtgtt
     8581 aatcgtttgt gtttgtgttt cattcccatt tacttgatac tgtggtgctt caaagttagt
     8641 caagtcggtt ctagcttttg cagtgtaact tgtaccagct tttactgaga catgttcttc
     8701 ttttagcagc gttccagttt gtcgattcat atgacggaca gttaccgcat attctgaagg
     8761 tttaggtttg tgttgtaacc ctttaaatgc agaatcaaga ctatttttag cattatttac
     8821 ttgggtttga gaagctttag aatcgtctag tactttttta gcattggata aagcagtctg
     8881 aaaattattc caagatcctt ctgtaaaatc tcctttagca gttgatttta cttggttgta
     8941 caaggcttgt aattccgttt tgttcacagt aggagctggt tttttcttta atcccttgta
     9001 agcagaatct aatgtgtttt tggcactatc cacttgtttt tgagtcgctt ttgagtcatt
     9061 taacactttt ttagcattgt ttaaagcggt ttggaatgcg ttccaggtat tgtcggtata
     9121 gtcacctttt ttagttccct taacgctcgt ataaagagct tctaaagctg atttattcac
     9181 tttgggttta gaggtgtcag caccatacca accaatacct tcgtatttcc agccatagtc
     9241 tttggttaac cattgtactt ctttccaact tgcagtgtag tgatgcgaac cagaggtaac
     9301 acctggaagg aaaaggcggt agatcggttt attatttttt tcggcagaac gccaggcagg
     9361 cccttcataa gtccaaccgt aatttttggt caaccatttc acttcatccc aactagctgt
     9421 gtaatggtga tcacgaaggc ctttattgta taaacgatag acaaggtggc caccgctagt
     9481 cggtgcttcc caagcggggc cttcataatt accccactta attttcacta ggtgatcacg
     9541 ttcttttgtg ctagaggtgt aaaaatgttc atggttattt ggattataaa ggcgatacat
     9601 gacgttagtt gaagcagcgt cagcagatcc agcaagagca actgtggcag caaatgttcc
     9661 aatagctata acagtttttg ggtttaataa tttcatgttt tcaactccct attaaatgat
     9721 taaatatttt gtttacattc ataatatacc acggtatacc gaggtagtca acggtatacc
     9781 gtggaaattt tgaaataaaa aagccgccct atgaagagtg taaggatggc tgtttatatg
     9841 ctatgaagaa atggattcgg cgtatttggt tagtttggca gcattatcaa atcgcatttt
     9901 ttcaatcgca ctttttttct ttattaaatc cgcaattgtt gattctgcga ctgaagaatg
     9961 tttgctaatt tcatatgctg tcttgccagt ttcaaatagt aaccattcga ttttctcttt
    10021 atcagcgtac attgcaatgc tccctcttga ttttttcttt attatagctc ttttttgtaa
    10081 taaatcacaa tatcaagagg aaaagtttct atagaaaggt gggaaattat gagtatccgt
    10141 tccccgtgta tcaaaatgag gtttgttgct actttcgtta tttaatatac taaaataaaa
    10201 caacgaaaga aggaatagaa tgaaaaagtt aataatggta tttacttcgt tgattgccat
    10261 gattgttatt ttaggtggat gtggtagtga aaaaaataag caagatagtc aagataatgg
    10321 cgtgttaaaa ccaggaacag tgtggaaaga agaagtaggc ggacttgttc ataatttaaa
    10381 aattatagat gatacaacat gggaatattc cgaaagtgtt tggcatcctg aacaagtaca
    10441 aataacagtt gagaagctaa aagactataa aggattagaa agatataagg taatcgattc
    10501 aggtggagta gatttattca taggtaaaac agccttaata gttccctata acaaagacgg
    10561 tttagaaacg tttaaattta ttccaacaaa cgaaaaagaa catcgagata aaagtgaaat
    10621 attaaaagaa tacagtacta aatctggata caagttacaa aagactagcg agtgattgct
    10681 agtcttttta tttatcctga aggaggaatc gcagtggcaa aagctaaaac gccatttgaa
    10741 aaagccgcgt ctaaaatctt agaacgtcgc ggtgtatctg aaacacagtg gaaacaagaa
    10801 gtccttgaaa aagcacaatt tgacctatta cggggaaaag acaaggactt agaaggatat
    10861 atctatcaac gtgaacgtga aaagcttgtt ttcgatgaaa ttattaaaca ctaaaaggag
    10921 cgaacaattt tatgtttgaa tcaattacaa tgaatttttt aacacaaccg aaagtagtag
    10981 ctgcggttga tatttttaga gcattacaag gtgcagcaac tagtttaaaa aacaatctga
    11041 tgatttttgc ggctgtagct gccgttgcct ccattgttct tggggggatt ttcttcttct
    11101 ttggccgaag aggagctgaa aacgggaaga tgcacgtagg ttatgtattg atgggggtag
    11161 gagttatatt agctgccgcc tctattgtgg tttcgttatt tggaatgttt ggacaaaccc
    11221 cacagggact ataaaaaata aatagaagga gtaaaaagta cgtggctact ttttactcct
    11281 tttttatgaa aggagaatac tcatgtactt tacgatttca ggcaatacca aaaaagataa
    11341 agagttaata aaaaaagcaa cgaagaaagt gggaaagcgt actgcctttt taccgaaaac
    11401 aattaaatat tctgtcattg aaacggaaga acagctacat gtgttaggac aagtgttaga
    11461 acaagagaat caatcttctg ttaaattgac tctcacgtta catgataaag aaggaaaaat
    11521 agaaaaggct ggcacagaat acgtttttgg cccactggat tatcaaattt cagaaggttt
    11581 ttatcctaca ttgatggatt tgatccgcct tgatgtgttt aaggaaacgg atgaaaccat
    11641 gagtgtggaa gagtcttatc gaaacgaaaa actgctacaa gttttagaag aagagttaga
    11701 gagcgaccaa agaaaagtga gtcaagtgag tgaagtcgaa gtaaatgatg aagtaccatg
    11761 ggaacgacca atctttccaa agaaggagtc aaaagaaaaa gagacagtct atgttgacga
    11821 acaagctgat attccgactc agaaatcgat atttgaggaa gagaacgatg taatgggtca
    11881 attggaggta gatccctttg aagaaacaat ggaggatgaa gagacggccg aagtagaagt
    11941 tgatctagtt gaagctaatg aagagccaac atggactgaa ggggattctc aggaatatat
    12001 tgaaccactt atcagtgaag aagtagagaa aaacgaaaca gagatacaaa aggcaactgt
    12061 atttgattat attttggatc aatatccgcc agagcatgaa tggatgaaag aaaaaaataa
    12121 attattttta aatgagcttt attcgaaata tcaattaccc caaacctatc aagcattttt
    12181 agagagtaaa gaagaattaa tctcacaggg taaaggcaca cttttagaac agttagagtc
    12241 tgttcaatca acaaattggc atgagcttgc acaagaagat ttattagcca cttttgaaga
    12301 acgtcaacaa gaaacagatc aagaaataaa tgagtatgcg acggaacagt catctaattg
    12361 ggagagaaca aaagaggaac taaataaaga agaacaacga gtgattgaag aagaagtaca
    12421 aaaaattaaa gagcgatacg aaaagaaacg cgacaatgcg tattccttgt gtatagagaa
    12481 aattaataac tttactaaaa ctaatcaaga agctttatcg aaagaaaaag aaaacttgtt
    12541 aaaagaacgt ataaaagaac gaaaagaaga gttctaccga ggtttacgta cagataagct
    12601 aaagattagc aaagaattaa atgaacaact gaataaactc tatcaaacta cgactgaagt
    12661 tcttatggaa agacacgggc agatccagca agaattacaa gcacaaattc caaaatggaa
    12721 aaaagagtat gaaattcaac aacaagaatt gaaagaacaa gaagaaaaac aacgacaaga
    12781 agaaaaagag cgcgaacaat tagaactaaa aaaacgtgag cagcatcttg cagaacaaaa
    12841 agtggcgata gaaaaacaac ggttagagca agaaaaggaa aaagaagcag ctgctcgata
    12901 cattgcgaca agcacagttg aattatctag cacaacctca accagtagct gctgttcaaa
    12961 cgccagtgaa caaccaagca aatttaactc cttcaccaca gcgttccttt aacggatgga
    13021 tggtgggatg tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta
    13081 atcatatcaa tcaatcttca caggcagatt ctgtagccac aacggcgtca atcaaggaag
    13141 aagcccagga acaagcaaga aaagagtatg aacaacaatt ggaagaatta ttgaatcgta
    13201 ccaatcagtc aacagcagag tcaagtatgg aaaatagcaa agagagtgat cctagtaagg
    13261 ctaaagaagg atcaagcgat tcaactgtac actcaaacac ttccgaaagt tcagaatcaa
    13321 atgaggtgaa ataatgcgta ttagagaaac aacgagacga aaagacaaac gattcaattt
    13381 agaaaagaat aggtatcttt ctcatttcta tcgctatact ccttcaggag aagagctacc
    13441 agaaaaacat gacaaacgta tttctcaaaa gattttttat agtggcttag cttatcttcc
    13501 tttaaactat gttgttacaa gaataccaga agcatttcat cgatttgaga ttattccagg
    13561 aagtttacaa ggagtgaata tttttcagtt acaagaaatg ccgcagttat tccattttga
    13621 ttcaactggt ttttggaatc ctttgacccc aacaacaagt ttaggcacac gtttagttgt
    13681 ttctacgatt tgtgttgttc cagctgtact tatgggattg aaaatggaca tgtggtttcg
    13741 tccgctagca gatgggcaaa aaggagataa ccgattaaaa acgttaaagg aaatccaaca
    13801 aatttatccg caaataccag aatcaaaggg aagttttcct ggttatggcg gaataccaat
    13861 tactcattat aaaaaatact ggtatattca gacggataca gtaaatactt gtattgttgg
    13921 tacttcccgc tcagggaaag gccagataga agttttagca accattgaca atttgtcgcg
    13981 tgcagaaaga caaagttcaa tggttgttaa tgatccaaaa acagaattat atgttgctag
    14041 taaagatgaa ctagaagcta gagggtatga tgtgtatgcc tttaatatca ccgatccact
    14101 gcaatcaatg agtgataatc cattggcact cattgtgaaa tactggaagc gaggagacat
    14161 agacaccgca acacagttaa ccaatacttt tacgaatacg atttattatg atgcaaatgc
    14221 ttcagacaat aaatacttta acgaaaatgc gcagaaagct gtcaatgcgc ttatttttgc
    14281 gttattggaa caagcggatc aaacagggga ttactccaaa ttaacaccaa ataatctcgt
    14341 tgaactactt tcagaaattg gcggttttaa ttattatcca gatcctgaga atgaattcaa
    14401 gcagctcaat gccttagatg agttcatgaa tcagttaccc cctggaaata tagcaaaaaa
    14461 acaatatggg gcaacaaaaa ttggatctga taaagcgaaa gggaatatct tgtctacggc
    14521 aattacagga ttgaatccat ttaccctaac gaaaattgcg aagatgactt ctcaatcgac
    14581 cattgattac aagtccattg gttttccgaa gtatttggat ctcaaattac atgaaagcct
    14641 attaaatcaa cgcattgtcg tggaatttta tcataaggga aagaaatttc acgaggaacg
    14701 agtcaaagtt ggctatcgtg gtttttgtga aatcaacttt gattgtcatt tagagcaggg
    14761 agatcatgtt tgcttaaaat acaatcatca aggaaaacaa tatgttgctt ggtatgagtt
    14821 ttctcaaaaa gtattgaaag acgaagaggg aaaaatcgtt tatcgaaaaa aacgaggaga
    14881 aactcatttg ccagaactag aaaaagaagc cgttctaaca ttagatgaga agaaaagtaa
    14941 cctattagta gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgtt
    15001 aacgcctgat tatgatccat ctaaccatat cattgttagc atctttctta gtcagttgta
    15061 taaagaatta tctacgcaat gtgttaagac taaaggagat aaatgtcacc gtaggattca
    15121 ttttatttta gatgaatttg gaaatatgcc accgatggac aacatggaag gaatcatgac
    15181 ggttacggca ggtcgtaata tgttatggga tctgtttatt caatcttatc agcaacttta
    15241 tgcgaagtat ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta
    15301 tattatgtcg acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac
    15361 ggttgaacaa gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa
    15421 tgttgattca gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac
    15481 gattgtactc gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc
    15541 tatttttaat catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa
    15601 tccatcgtgg gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa
    15661 ggctttacaa attgattatg attatttcct accagatgaa tatgctagaa atctctatca
    15721 tggactgcat gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca
    15781 atcttttgag aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcaac
    15841 ggatcgtctt caagaatatg ttgaaaagtt gatcaaaaat gaagaaattt ctgaaatgat
    15901 taggaaacgg ctgttattgg aagctgaaaa ggcggtgaaa gaatgatcat tggaggaatt
    15961 ttctctggca tagccgactg gtggtcaggt ttattcggag aaggggatgc agatcccaaa
    16021 accgtaacga aagtcttaca agattatggt aattatctgc agtatcaaga tgtattcagt
    16081 tggatttgta atactttttt atggggattg atcaaagggt tatataatct caatacttta
    16141 ttagaaaaaa caatttatca aagctttgat ttaaaagact tattgaacgc cgctggtgtt
    16201 aatgaactat ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta
    16261 atttatttag gcttgaaatt ttttatgagt aaacacccac cgaaagtcaa aaatatcttg
    16321 gttaatttat ttatggccat gatcttgatt tttggtggta gttctttaat cgatgaaggg
    16381 ttaaatatca gtaaaaactt ttatggagac gtcactgcag caaataaagc agataaaacg
    16441 ggttcacctg cttttcaaat tattcaaaat aatgtgattg atgtcagtac attactagcg
    16501 actgacccga ataaagtaga atccttacca acagataaaa gaaatgcttt aacagcgaaa
    16561 aatttcaaaa cagcgaatat caatgggatt attacacctg atcaagctaa gaaaatgtct
    16621 gaatcaagtg atattagtac agtacaaaaa gatcggttaa aggcattaca gtatcgacta
    16681 gagttagacg ataatgggaa agaaatccct gttcaaatta gtgatgaagg tttagcaaaa
    16741 tatgtgtata cgtcaggcta tcgtcgatac attagtagtc cagggattat tttggctggg
    16801 gaatcttctt tgtcggtagc ctatttattt attttgttta caatcgttac ttgcattatt
    16861 gagctagtat ttaaaaaatt ctatttagta attgccgcgt caacggactt tgagacagga
    16921 caacggatga aaagtgcgat agaagatatt tctcaatcct tcttgttatt ggcgtttacg
    16981 attttagaat tacgaatcta tacattaatg ctttcaggta tcggtgattt gcatgcggta
    17041 ggaaagatca atggattctt gtatgttgtt gctttaattg tgctaactat tgcgctattc
    17101 aaaggctctc aatcggttac gaagattttc ggtgttgata cgagtctgaa aaacggcagc
    17161 aattctcttc ttagcatgtt tgccttagga aatattgcga aaaatgtggg tagcggagct
    17221 aaaaaccttg caagtgctgg taagagtggt ttaagccatt taaatgatat tcgtaagaat
    17281 ggatttggcc atcgaaaaga tattacccat gataacgggg aagaacaatc agacgataaa
    17341 gctacaactg tacctaatag taagaagaca gtttccgaaa tgttcgggaa agcgaaaaat
    17401 ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt aaaaggaaca
    17461 gttgaagatc ttacagaaaa agccaaagac accttagaag agaaattaga cggcacgaga
    17521 gctaaagaat ttgctgaagc tgtgaagaat ccatccgaag cactagaaaa acaaaaagaa
    17581 aaagcttcag cagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca agtcaatgca
    17641 gcagtgaagg caaatacaac aagtgaaaaa gaaaatcaag aaggtgcagc gattccttca
    17701 gccaaagatc accaagatga gttacattct aacgaaaatt cgatcggaga aaaagattca
    17761 gaagaaggac aacatggtca gttaccaaaa accgatccag aacaatatcg ttataaatct
    17821 aacattaatt ctgaaaacga aaaacgcatt tcagatttac caaaacgaaa tcaagaagta
    17881 caaacggatg aggattccat cgatctgcca aatgataatg gcatcgaagt tggaactgaa
    17941 aaatcaacga aaattggttt gcctaagaca caaaatttac caatgaaaga tagtcgtttg
    18001 aatcaattca aagagaatga tcaaagcttt gttcatcaat cacctgcagg tactgttcaa
    18061 atgaaatcaa gtcgtttgcc tgaaaatgga aatctgacaa ctgtttctta caatttagct
    18121 gaaagtggtg aaacagagaa tgcgtccgtt caaaatattg tgacaaaaga agctgcacct
    18181 aagccaatca ataaagttca taatggatac agaccagcaa acgtagcatt tactgcaccg
    18241 acaaaaataa atgtgacaag agaaactaaa aacaggaaca atgaattgaa agaacaacta
    18301 ttgaaggata tcaaaccata aaaggcctat ttaagggcct ttttctttat aaaacaagtt
    18361 taaaatggaa aggagaaaat tgtggaggat attttaatag ctacgtttat aactgcactg
    18421 ggagcatttt taacgatatt tgtggtgact attaaaggat ttaaagatac ggatagttta
    18481 caaaaagatc atcagtctat tttagatggt caaaaaacac ataccgaaag cattattgta
    18541 cacctttcag aaggattcac ttatttgaaa gatgctatgc ccacattcac agtaaaatta
    18601 acggaaatat ttcattctgt tgagcaaata tcgtcagaat tgaaagagca aagacaacaa
    18661 aaagatcgtc aacttggctc acttaatttt aggcaagaaa cgatacaaga atcattggag
    18721 aatgtaaatc gattaaacga tgaaatgcaa cgagttcatt tggaaaatga agaattacgc
    18781 gcacaaaatt atgatttaat tcaaaagaaa agaaatctgg aacaaaaaat tgttgaatta
    18841 aaagaagaaa atcgtcaatt gaattatcaa ttacgacgag gaaaaaatcg ttctcgtgat
    18901 ttaggaaatg aattagagtt gtaatattta gaaaaacgct gtgagaaatc acagtgtttt
    18961 ttatttctaa aaatacaaaa tataaggggt atggttatat tatggataaa tacattcata
    19021 tgaaaaatcc tccaagattt agtggggaat ggaagttttt cgcctttttc tttttagatc
    19081 tattaacgct tggtggctgc tttttttggg cgaaaatagt caataatctt gtccacttac
    19141 cactcttttt tgcactcttg aattatggat tatggcttgc gatagcgtta gtgattgttt
    19201 ggcgacccta taataaccct ggtcaacgcc aaatttttgc gattatcgaa tctattttct
    19261 ttacggataa gaaccactat catgcgttag atcctaaccg aaattatcat ggtacgtata
    19321 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgataagaaa tccattttgc
    19381 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    19441 aagagggagt cttaattact agagaagatc attttcagcg ttattatcgt gtcgtttcca
    19501 ctgacgtgga gggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    19561 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    19621 cggaacaaat cgtgcaaaag cggcagttat tagataaaaa tcgtatggaa cagtcactag
    19681 gaagaaatct acagcgatta agaaaatatg aaaagaaatt agtagaggag atccaggaat
    19741 taaaacgggc agaacaagaa cgtcctgact taagcttctt tttcgttatt gaaggaaaga
    19801 caatcaagga tttacaaact agaaatcgtc agttaatgcg atctagtggc attttagggt
    19861 taaaaccact ctacaaaaaa gaattgatta agattcttta tcgaatgaac aacatgaatg
    19921 acgaataaaa aagatggagg aaatgattca tgctagatgt gataaaaaca gctttttcaa
    19981 cgcaaagtaa tcaaaaggtg attgatccat taaaacaact tggtaatcgt gaaatagaga
    20041 ttttaaaatc aaaaggttat gatttagatt tcttaaaaca agtgatgcca caaggtgggt
    20101 tgaattttga tgaagaatat gtgacctatg gaaatggtta ttcacgatgt gtgacagttt
    20161 atcattacac aaaaaatcct actctttttt ggttggccaa tctcatgaac aatcctcata
    20221 cagtggccac actagatgtt agcacagatg aaaagcaaaa agtgattgat caaatcaacc
    20281 gcgcattaga tgaattaaat gatcgatcag ataatgaacg aaaagcgact gaccgaaata
    20341 aaagtgcgga agaatacctg gaactagcgg aatatgcccg aaaattgact tctaatgggg
    20401 aaatttctaa acaagtcaaa gtgagagtac atgtttacgg aaatacgatc caagaagtgg
    20461 atgaacgggc gcaggaactg atcgaaaatc taaaagggat ggattatcgt gcggtgactt
    20521 atctgcataa aaccaaacga gattggcaat cgatgtttac catgttcgat gaacaagctg
    20581 aatggtttgg aaatatcaaa gggcaatcac ttccatcgtt aaacattggc ggaggattcc
    20641 cgttccacca ccaaacgcta aaagatccag gaggcacaca atatggggaa accatgacag
    20701 gcggcccatt tgtctggaat gccacgtata aaaatagtgt acgtttatct accaatacct
    20761 tgcttctagg aatgatggga gctggtaaat cgaccgttct taaaatgatc gcggaagccc
    20821 atttagctgc aggagacttt atttggggct ttgaaaaggg aaaagacttt attcctttct
    20881 taaaggaata taacggtatt atggtgcgtt tagacggatc tgacgggatg atcaatcctt
    20941 tagagatctt tgccacacgg acgtatgatg aagcatccag tttatatgac gatggttcag
    21001 tcaaagattt gaagatcaat gaagcagcgt cctatcaaac gcacttagac aaagtagtct
    21061 atcaggtaca gttagtgagt cctcagctaa aaggaacgat gaaagcagaa ttcaagacct
    21121 acttgaatcg tttttatgaa gagtatggaa cagtaccacg cggttttact tcctcaaatt
    21181 cgcgttcaaa tactgatacg caagtcacag ggaaagatcc agaagcttat ccgacgttca
    21241 aagaattttt agccttttta ggacaactgg aactaccagg ggcttcccaa gagaaaaaga
    21301 atcggaaaga agaaatggag tctatcgtgg aatccttatg tgagacttat ggcatgattt
    21361 ttgatggtca tagtacgatt cgacaccttg accaacaaca gttggtcttt tttgacattg
    21421 actctattac aggcctagat cgaggggtac aacaatgtca gatgtacatg gccatgacgt
    21481 tgatttggaa tcaggcattg attcaaggac gaagacaacg acgcttaatt aaagaaaaac
    21541 aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc cataatgcga
    21601 ttaatccaga tttcattgaa acggtaaatt atgtcacaaa cttccaacga gaaatgcgta
    21661 aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg ccagaaaata
    21721 tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct acgaagatct
    21781 ttatgcgtat ggatgaatct attttacgtc atatagaagg attagttggt aaatcaatga
    21841 cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg atcaattttg
    21901 gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta aaattgtttg
    21961 atggaggaaa ataatggaaa accaaaatga aagtttaatc aaacagtatg ttaagcgtcg
    22021 tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct gcaggtttga ttactatatt
    22081 gatcactgtg tttgtgaccc tttttctaat acttgcggca ggttctattg ataattctga
    22141 ttcaagtagt ggtggcgaag cttttacagg agaatattcg gaaggcttac ccatttataa
    22201 agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgctg taggtgcagc
    22261 agtgaagtat aaattattac caagtgtgat cctttcgcaa tacggttatg aatcagcgtt
    22321 tggtacgtcg gcgtccgcaa gaaatgatct caattatttt gggattacgt ggtttgatgg
    22381 gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg gctggtacat
    22441 gaagtttccc aactcaaaag ctgccttctc ctattacggc tttatggtag ccacacaatc
    22501 aaattttaat gcttgtgtcg gaaataaaag ccctggcgca agcttattga tcttaggacg
    22561 aggagggtat gccgccgcag gaattactga agacagtgcc tattataaaa attgtatgtc
    22621 tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac actggagaga
    22681 aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc caggaagtag
    22741 cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag gaggagaatt
    22801 ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc caggaagtga
    22861 gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta tttcaggttt
    22921 gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc aagaatttgc
    22981 aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg gtcaagtaat
    23041 tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt ggcgtcaagc
    23101 acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat ttttgaatag
    23161 cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa accttaaaaa tcgtcttaac
    23221 agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc ttaaagaaga
    23281 acaagagaca aatacctata aaattgaaga agtggctaaa aacgcgtctg ataagctcaa
    23341 aaaaaattct tctgaagcac aggcgaagat tgacaagtta aatcaaacca tcaatgagaa
    23401 aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag aggaagtaac
    23461 tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata aatcattaag
    23521 cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga tcgacaaatt
    23581 aagtcctgta gacgatgatc accaacatga tgattacgga agttactctt ttacattagg
    23641 tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt ttgtcgtatt
    23701 tgttgattat acaatcggca atcctcaatt caaagatgtc aaaccacaaa aaataaaagg
    23761 tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag actttagtta
    23821 tttcactcgt taggaggaag aaaatgagta agaaaaaagg agcaagacaa cgttttaaaa
    23881 aacgaaaaag ttatcttaga tggctgggga tctttatgat tctcggcttt ttgattcttg
    23941 ctagtccgat ttacacacat aaaaaggtag acataccaga tgaagtaagc acgaacgcat
    24001 ttttaaacac gaaggaactg acgatggttt caaaagaaaa aggagcgaac cagcaattag
    24061 tttttgaatt tgcgatcgac gatgaagatc agagcttatt aaaagaacta gctaatctta
    24121 attatcgtgt agaagtaaag acagctaaag gggattatca agcaataaaa gccaaagtga
    24181 tcaaggtgtc tgatgattat tttgtagtga agttagcaca tgtaccagaa gaatatattg
    24241 ctatgcgatt aactattatt cctgaaaaaa ttgatccaaa ggtggatatg caagaaccgc
    24301 aagatctgat tttttatatt cacgaagata aggtcaaaga tcacgtgaaa gacgaagatt
    24361 atgaacaaca tgcgatcaat tacaaagtga aaggatacaa gaaagaacaa aaagcagcac
    24421 aaaaacaaat tgaatccttc caagcaacca ttgatttgaa tgaagaatta gtgaagaaac
    24481 taaaagatca attgccttat caagtcgcag aagatcagga aaatactgaa aataagatta
    24541 atagttacca gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa gaagagagca
    24601 ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaatatttag aagaataggt
    24661 ggagtaaaag aaatgaagcg agcgaacttg gtcggcaaag atactttcgc tgagcagaaa
    24721 gtattgatta aagcctatct caaagttgta aaagctaaag aaatatgtca agaagtgaat
    24781 tatctaacac aacaatatct gagttgaaga atggtagaaa gagtttagat accttaaaat
    24841 tcagatcatt tgaagctcta tatatagctg ctaaaaaata ttttacagct gataataaat
    24901 aaaaaattaa ggtgggttct atgtgaaaaa aacgtatcaa aagtttttat ggattagcta
    24961 tatcgtccta tttatcgttg ggtttctcgt cagtgtgtcc gtgatcgtcg gaacgctttt
    25021 tcttttgttc tctgacgaag atctggtact taaggggtta atgagtacgt tatcgtgtgt
    25081 catgttactt tatttatgta tacagatcaa cgaaattatg ccccctcatt ggcaagaaaa
    25141 gaagaaagaa tttaaggaag aaatcgaaat aaaatccaat gactggattg aatacacatg
    25201 gcttcctctg aaaagaaata gcaaacgaaa ggaattttgg gcattgcaat tatggccaat
    25261 aggaaaactt tttatttata tggcttcact tatggttact ttaatcggtt atttggtggt
    25321 ggtctatggg gtggcctatt acttgttaag tgagccaaaa cagatcgcgg cgtcaaatat
    25381 tttcacggca gtaatgacat taatgtgtgt catgtgcttt tctattttta gttacttact
    25441 cgtccaattt atcaggcaaa atttaacaat gagtaaactt ttcaaaagtt tattggactt
    25501 acttatctta ggcagtatgt taacgactct aacagatcgg ttcgaacaat ctgaaaaaac
    25561 ggtagagggg ataaagaact tcttaacgac gattgatcaa tttataaatg gatatatgta
    25621 tttgtttgaa gtaggcttta ttctcacttt gtttggttgt gtatttataa aactcattaa
    25681 aagatcaatc gaaaaagaaa tggttattga tctaattccg acgctgagcg gctatgagta
    25741 ttacgttgat tttggaaagt atgaggagtt gatccatacg gatatcgttc acacgagtat
    25801 ccatgaagta accgttaagg gcatcagatc tactttaaaa gatggacagg agacgaaaac
    25861 aatcgaccga agaaaagagg tctacaattt ttataaagtt gaagcaagtc tacgatatgg
    25921 cattgggaaa ttgaggttag atgggagtaa tattcaacaa aacaaaccat atgttttagg
    25981 aaaagtggtg atttagaact tcctatgaaa gatgaataaa taaggagaat cttttgaaaa
    26041 tgattcttct ttacagaatt gtgcttatag ttggcacgga gttaaaaatc ttaaagaagg
    26101 agcagttatt caaagtgaaa gaacatcata atcagttact atgtattaaa acgggtacga
    26161 tgaacattca ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgtcga
    26221 ttcgagagca acataagcaa gaaaaattaa tggctactta tagccaattg attgatgagt
    26281 gggaacaatt caaggaatct aaaacataaa attccattta aacaccttat tttttaaaga
    26341 gcataaggaa acgtgtaccc actttcaaaa taaagtgggt agataggctg tgcctatcac
    26401 gtttctgaaa aaaataaatg accccaaaat tttcaattta tgtgtaggtt tatgggtagc
    26461 aaaaatatga atgtgggtag cacaaaagtt catttgtggg tagtaaaatt taaatatgtg
    26521 tagcaatgtg tgtagctaca cacattgcta cacacgttat aacatatagt caaaaggaga
    26581 tatttatgga agtaaaaata agagatattg atgaaaaaac gattcaaaga attgaccgaa
    26641 gatgtattga attaagtgaa cgtactggga ctaaatggag ccgaaatcga tacctaaaag
    26701 ttttgatcga aaatgatttt gatcacgctc ttctggatta taaaaaagat caatttgatc
    26761 ggttgttaga aaaatttgtg gatattcaaa cttataatac gaaaatttta gaagaatata
    26821 ttgcaacaaa taatcaatta atcgccttgt taattgaaca gtaaaaataa tattgtgaat
    26881 ttataaaagg aggaaatcaa ctttggcgaa agcaacaaga aaacatattt atctttatga
    26941 tatgcatatc gaagtacttg atgagataat ttctactcat gacgatatat ccaattatag
    27001 tgaagcagtt agacatttgt gtatgatgta tgatcccaaa actgaagaaa agataagtcc
    27061 cagtcaaatc aaactaaatg caattggaaa agaagtttct attttaactg aaatggtttc
    27121 ggagtttgca gatcttcatt taaaagatac aggaatatta attggtacaa attcaactgt
    27181 ttatcaagaa gcgaaagagc gtgtggaagg caagataaaa gcaaatcaaa cgaagaaata
    27241 ttctcctaag ataaagaaaa caataagcga atcagtgact acaaaaagtg aagtatttga
    27301 tccaacaagg tttttaaaac gataaaaatt agaaggagaa cgtgatgagt aaatcacctg
    27361 cagtcattct cacttgtcaa tttaccttgc ccaataaaaa atctttttca acatacattg
    27421 attatatgac aagagaaaaa gcgctggaag aaatggaacg gcgcacgctc gaagaagaac
    27481 aggaactgca acgaatcaag aatgctttag aggattttta tattcctgaa ggagaaactt
    27541 tttcggagag taagacggat aaagaattca gtgaaacgtc gaatgaagcg caatcgttga
    27601 tggaatatgg aatcaatttt gacaagttag aggaacaaga ttttaccaaa tatctttctt
    27661 atatgacgcg tcactatgcg ttagaacaaa aaaaggaact aacggaaagg gaaaaaatag
    27721 aatatcaacg tttgaatcaa gcaatggaaa aatatcaagc agaaccacac aaaaaaactt
    27781 caggaactct tcctggagtt ttttctatat cttcagacga agtaaaagga agtgatctga
    27841 aggagattaa gcagatcttc caaagagggc aacaaaaagg ctcaatcatt tatcaagatg
    27901 ttgtttctca tgataattcg tatcttgaaa aaataggatt gtacgatccc aaaacagata
    27961 ctttagatga agttggacta aaagctgctg gaaggaaaat gatggagacg ttgtttgaga
    28021 aagaaaaact gaatgatacg ggatattgga tggctaccat tcaccgtaac actaagcata
    28081 ttcatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccatac actgaggtcg
    28141 aaaatggtgt gagttatatc gtaccgaaag gaaagagaag acaagcaaca ttagacgcaa
    28201 tgaagaccaa ttatgtgcat gaattagaac gtttttctta tgaaaaggat cgaatgatag
    28261 gcatggagaa acgaaattta ttaacgcgga aaagtgattt gcgtaacagc ttaacaaaag
    28321 tggtaaaaga gccagtacgt tatgatcgac acgcaatgaa cttattaaat gaagtatata
    28381 aaaccttacc tgacaaacgt tcggaatgga attatgggga tgaaaatcga actaaacttt
    28441 caccatacac aagagagaaa ctcgacgagt taacccaata tgtgttaaca aaagaacctg
    28501 aataccaaga atatatacag atttcaaaag cattgaaaga agaagcaaaa agtctttatg
    28561 gggaaagcaa gagagaatca aaggatgcag agaaaaatgc tctctttgat ttaaaaaagc
    28621 gcacaggaaa tgccatccta acagagctga agagacagga tagtcaaata aaaccattaa
    28681 tagaaagtgt tgatcagacg caatctattt acaatgaacg atcctttcga acgcttaaac
    28741 aattgccaac gaatgaatac aaatatagtt acgaagaatt catgaagcgc aggaaacaat
    28801 tccaacaaag agttgtcacg cagcggagtt tgcgtcaatt aaatcgaacg attaatgccc
    28861 atgaagaaaa ataccacgca agtaaagcat acgaagagca acaacgaaga atccaaatgg
    28921 aacaagaaag acagtaaaga cagtaaagac agtaggagga atagcaatga aacaatattt
    28981 agatcaatgg aaagttatag aaggatcatt aagagaagaa cgaatcgaac aacttcccga
    29041 ttgtttagaa aaagagcatt tgtttcagat aagagaaatg ttgataaacg aacagtttga
    29101 tccaaatcaa tttttagtcg ttgagtaccc agctactggt gtgtactgtt gtaatcatgt
    29161 gaagggtgaa aaatatttta tcattcaaga atatgagggc aagttagcgc cttattatac
    29221 tacttgggaa atgaatgaag aggggatcaa taacttcccg tgtaaaagca ttgaagagtc
    29281 tatttcactc acagaatgtt agttgttaag tgaataaaag aagtaaaaga gtcctgaacg
    29341 tgattgtcca ggactctttt ttttcactga attgttttcc gccaattcat tgagtggtgc
    29401 gatttccgtc tacattatat caaaagaaag gaggaagaag aaatggctta ttcaacgatt
    29461 ggagttgttg ttgaaaaaag cagagacaat cttgtttttg tgacagaaat tcaaacaggc
    29521 agagcttttg tagtcacaga caaagcggca aaagcatatc aaagtggcga tattttaacg
    29581 ttaaatctga ctacgaagac gttcgtcgat gcggcagaag attatccatt
//
LOCUS       DACEDM010000038        29101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEDM010000038 DACEDM010000000
VERSION     DACEDM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.038"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1028)
                     /locus_tag="IWO50_001479"
     CDS             complement(<1..1028)
                     /locus_tag="IWO50_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0358606.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1110..1799)
                     /locus_tag="IWO50_001480"
     CDS             complement(1110..1799)
                     /locus_tag="IWO50_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0358607.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1905..2273)
                     /gene="rplL"
                     /locus_tag="IWO50_001481"
     CDS             complement(1905..2273)
                     /gene="rplL"
                     /locus_tag="IWO50_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0358608.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2326..2829)
                     /locus_tag="IWO50_001482"
     CDS             complement(2326..2829)
                     /locus_tag="IWO50_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0358609.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3239..3928)
                     /gene="rplA"
                     /locus_tag="IWO50_001483"
     CDS             complement(3239..3928)
                     /gene="rplA"
                     /locus_tag="IWO50_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0358610.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4032..4454)
                     /gene="rplK"
                     /locus_tag="IWO50_001484"
     CDS             complement(4032..4454)
                     /gene="rplK"
                     /locus_tag="IWO50_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0358611.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4797..5465
                     /gene="sdaAB"
                     /locus_tag="IWO50_001485"
     CDS             4797..5465
                     /gene="sdaAB"
                     /locus_tag="IWO50_001485"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0358612.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5478..6350
                     /gene="sdaAA"
                     /locus_tag="IWO50_001486"
     CDS             5478..6350
                     /gene="sdaAA"
                     /locus_tag="IWO50_001486"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0358613.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6441..7460)
                     /locus_tag="IWO50_001487"
     CDS             complement(6441..7460)
                     /locus_tag="IWO50_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0358614.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7633..8520)
                     /locus_tag="IWO50_001488"
     CDS             complement(7633..8520)
                     /locus_tag="IWO50_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0358615.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="IWO50_001489"
     CDS             complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="IWO50_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0358616.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9243..9413)
                     /gene="secE"
                     /locus_tag="IWO50_001490"
     CDS             complement(9243..9413)
                     /gene="secE"
                     /locus_tag="IWO50_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0358617.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="IWO50_001491"
     CDS             complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="IWO50_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0358618.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9751..10395
                     /locus_tag="IWO50_001492"
     CDS             9751..10395
                     /locus_tag="IWO50_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0358619.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10451..10954)
                     /locus_tag="IWO50_001493"
     CDS             complement(10451..10954)
                     /locus_tag="IWO50_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0358620.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10967..11013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11551..12516
                     /locus_tag="IWO50_001494"
     CDS             11551..12516
                     /locus_tag="IWO50_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0358621.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12615..13787)
                     /locus_tag="IWO50_001495"
     CDS             complement(12615..13787)
                     /locus_tag="IWO50_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358622.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13774..14919)
                     /locus_tag="IWO50_001496"
     CDS             complement(13774..14919)
                     /locus_tag="IWO50_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358623.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14933..15865)
                     /locus_tag="IWO50_001497"
     CDS             complement(14933..15865)
                     /locus_tag="IWO50_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358624.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15982..17061
                     /locus_tag="IWO50_001498"
     CDS             15982..17061
                     /locus_tag="IWO50_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0358625.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17058..17687
                     /locus_tag="IWO50_001499"
     CDS             17058..17687
                     /locus_tag="IWO50_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0358626.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17797..18024)
                     /locus_tag="IWO50_001500"
     CDS             complement(17797..18024)
                     /locus_tag="IWO50_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0358627.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18127..20715)
                     /locus_tag="IWO50_001501"
     CDS             complement(18127..20715)
                     /locus_tag="IWO50_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0358628.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20735..21394)
                     /locus_tag="IWO50_001502"
     CDS             complement(20735..21394)
                     /locus_tag="IWO50_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0358629.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="IWO50_001503"
     CDS             complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="IWO50_001503"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0358630.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22167..23033)
                     /locus_tag="IWO50_001504"
     CDS             complement(22167..23033)
                     /locus_tag="IWO50_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0358631.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23142..23783
                     /locus_tag="IWO50_001505"
     CDS             23142..23783
                     /locus_tag="IWO50_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0358632.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="IWO50_001506"
     CDS             complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="IWO50_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0358633.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25224..26123)
                     /locus_tag="IWO50_001507"
     CDS             complement(25224..26123)
                     /locus_tag="IWO50_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0358634.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26125..26922)
                     /locus_tag="IWO50_001508"
     CDS             complement(26125..26922)
                     /locus_tag="IWO50_001508"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0358635.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26900..27604)
                     /locus_tag="IWO50_001509"
     CDS             complement(26900..27604)
                     /locus_tag="IWO50_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0358636.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27735..28310
                     /locus_tag="IWO50_001510"
     CDS             27735..28310
                     /locus_tag="IWO50_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0358637.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28428..>29101
                     /locus_tag="IWO50_001511"
     CDS             28428..>29101
                     /locus_tag="IWO50_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0358638.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8560 a         6169 c         5232 g         9140 t
ORIGIN      
        1 acagtattat ttcggttgct tgctgcttca cctagccctt gtactcgtgt ttgattggcg
       61 acttgaccaa attgattccc gacgtctaat aaaataatag ctaaaagcaa aatggccaaa
      121 tggcttccgc caaccaataa agcaataaag cttattgttt gcataacaag tcccatatac
      181 acgatttttc tttcagaata agaatctgcc agtcgtccca cgataggtgc agcaactgct
      241 ccagacaaac caaaaatcgc taatatccct gcttcgaatg ttccccaatt gtaggcggga
      301 ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat
      361 ccactgactg ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg caaactttta
      421 agtgaatcaa ggtatgaaac agctgtacta ctttgctgga cagaacgcgg cattttccag
      481 taaagcagaa gtgtaagaat tccgactgca cataaagcaa ataaatagat catccgccaa
      541 gaaaaaagac tggcaacaat accagatacc gtccgagaaa gcaaaatccc cacgagtaaa
      601 ccgctaagca atgtacccat gaccttccct cttgcttttg gtcccgctag taccgcaccg
      661 taaggaatga tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca
      721 aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag agacggcaga taacgctacc
      781 attcgaatga tcaattttgg acgatccacc acgtccccta aaggaactaa aaacagtaac
      841 cctaacgcat agcctaattg ggtcaacatc gtcaatatcc cgactgcact tgtgctaaca
      901 gataaactag ctgctacttt tgttccaatc ggctggatgt aatacatatt ggcaacaaca
      961 acgccacaag tgattgataa tagtaaaata agcgaagttg ttaaaggtgt tttttcatta
     1021 ctcttcattg atttccccca tttcctatct tagtttacaa cttatggaaa agattggaaa
     1081 tagaaaaata caactattcg ccacacttat tatttgtgct tttgttttcc ttgttttttc
     1141 ttgcgatctt tataaatgaa gacaactaac cctactagca cagtaacact tgcaaccaac
     1201 gtgactgcag ttttgttata tccgtgaaac agagcttctc caccacacgc atacaacaca
     1261 gacgaaggga tgactccaat cacgatcgac aatatgattt gctgtagagg caatttcatc
     1321 gcatctgcag caaaattgac catagaagat ggaataaccg gtatcatata gcctaacatt
     1381 acacctattt ttggatgttt catttgtcgg attgcttgca cccaatgatt cgtttcggtt
     1441 ttcttatcta aaaattttaa gcgatgcata aggaaaattg cagtgatgtt accaagagca
     1501 ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa agctcaaacc agcaatgact
     1561 ccaactactg aagtaggaat acctggaaca gcacacatca ccgctgtcaa tacgatcaag
     1621 agtatcgatg caaaagtccc atgcgaacga atatgtgcga gtagctctgc cttactcgct
     1681 ctcggattta aaaataattg gatgtctgtc tgatattcta aatacaaccg atagcccact
     1741 accagtatca gaatgattcc caacgagatc aacatcaatc ggattatttt ctttttcata
     1801 tttcctcctg aaaaaaatct atattcatta aataatagtt taacataaaa acaaaaaagc
     1861 accgaaatcc aacataggat ctcagtgctt ttaaatagta aaaattattt aacagttaca
     1921 gatgcgccaa cttcttcaag tttagctttc aattcttcag cttcttcttt agatacgcct
     1981 tctttaactg gtgcaggagc gccatcaact acagctttag cttctttcaa gcctaaacca
     2041 gttgcttcac gaactgcttt gataacttta actttttggt ctccagctgc agttaattct
     2101 acagtgaatt ctgtttgttc ttcagcagca gcagcgccac cagcagcagc tacagctaca
     2161 ggagcagcag cagatacgcc aaattcttct tcaatagctt taactaggtc gttaagttct
     2221 aggatagttg cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc cattataatt
     2281 tcctccgttt aattgttctt ttttatttaa tgctttatgc ggcaattaag ctgcatctcc
     2341 atcttctttg tctgccactg ctttgacagc gtaagccacg ttgcggactg gcgcttgtag
     2401 tacagatagc aacatagata gaagtccttc gcgatttggc aattttgcca atgctgtgat
     2461 ttcttccaat gaagagactt taccttcgat gataccgcct ttgatttcta atgcttttgc
     2521 ttctttagca aattcgtcca tgattttcgc tggtgcgact acatcttcgt tactgaatgc
     2581 aacagctgta ggtccagtga atacttcgtc catgccttca aggccagctt ttttagctgc
     2641 acgtgaaaga atagaatttt tgataacttt catttctacg ttagcatcac gaagttgttt
     2701 acgtaaacgt gttacttcat caactgttaa tccgcggtag tcaacgacta ctactgatgc
     2761 agctgctgta aatttttcgc ttacttcatc aacgattgct gctttttttg cgataattgc
     2821 ttcactcatt tttgagtttc acctcctgaa ttttgatgga agagtttttt gcactgaaaa
     2881 ggcaagtcac ttttatagtt actttgctca accagtatta ttcatcacct attcacttcg
     2941 ttcatagtga ttctactcgc tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt
     3001 attgttcatc atctcttcac ttcgttcata gtgattcaca ataaaaaact ctatgccacc
     3061 gcagacatag agggactatt gaatatattc aataaacgtc ctcggtagga aattaaggct
     3121 tgatgccacc tactgtcttc ggtacaagtt aattattaac ctcaactagc ttagcatgct
     3181 aaactagttg agtcaagaat tatttttcag ttggatcaat caacattcta atatttaatt
     3241 agaaagaagc ttggtcaaca tggataccag gtccaaatgt tgttgtgatc gttacatttt
     3301 tcatgtattg acctttagct gctgatggtt tagcttttag caatacatca tggattgctg
     3361 caaagttttc aactaatttt tcatcgtcaa atgaaacttt tccgattggt acatggatat
     3421 ttccagcttt gtccacacgg taagttactt taccagcttt cacttcgttt actgcttttg
     3481 ttacgtccat tgtaactgta ccagttttag ggtttggcat taagcctttt ggtcctaata
     3541 ctcgtcctag acgacctaca gtagccatca tatcaggtgt tgcaacaact acatcaaagt
     3601 caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag
     3661 ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac
     3721 cagttccgtt tggtaataca acagcaccac ggatttgttg gtctgctttt ttagggtcta
     3781 cgttcaaacg gtatgcaact tcaactgttg catcaaattt tgcaatgttt gtttcttttg
     3841 ctaaagctac tgcttcggca acaggataaa ctttacttga atcaactttt tttaatgcat
     3901 cttgcatttt tttgctcttt ttagccattt tggttcctcc ttgattgtgg ttataacggt
     3961 tgtacctccc acgtgttcta tatctttcaa tatagagcca actgagaagc tgctgtctta
     4021 gggtgtatag attattctac agtgatcccc atgcttcgtg cagttccttc aaccatgcgc
     4081 attgctgctt caacgttagc tgcgtttagg tcttccattt ttaattcagc gatttctctt
     4141 acttgatcgc tagaaacttt cgcaacttta tttttgtttg gttcgcctga acctttttcg
     4201 attttagctg cttttttcaa tagaacagca gctggcggtg tttttgtaac gaatgtgaat
     4261 gaacggtctt cgtatacaga aataacaacc ggaatgatca aacctgcttg atcagctgta
     4321 cgagcgttga attcttttgt gaatcccatg atattgatac ccgcttgacc tagtgcagga
     4381 cctactggtg gagctggagt tgctttacct gcaggaattt gcaatttaac gattttttct
     4441 actttctttg ccacgagaca tacctcctta agtccgtgat gtggttaatt ggagatgcat
     4501 atttctcctc ccacgtttta tctgatctga aaaaatccag acaaataatg tgcaaaacaa
     4561 tgcacactgg tacagtatag caaatttatt tgttttttca agattttttt ctgctatttt
     4621 tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt ttcgttctca
     4681 tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc aaacaataac
     4741 acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg gagaaaatga
     4801 aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt ggtccaagta
     4861 gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata tttggcgaac
     4921 agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat cgggggcacg
     4981 gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat gaacgtttag
     5041 ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt cctaagagtg
     5101 aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt
     5161 cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg aatggattca
     5221 aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat gttccaggta
     5281 tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc actatgacag
     5341 tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat gatcctcaag
     5401 tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta aattatttcg
     5461 attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa gcaaaagctt
     5521 atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc cgttcccgta
     5581 gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg
     5641 gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg
     5701 attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct gtacgcaatg
     5761 ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg
     5821 gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt gatttgacac
     5881 atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca
     5941 acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct gcaagtgcaa
     6001 tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag
     6061 tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca ggcttagttg
     6121 aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc tctgctgata
     6181 tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct gcaatgtacc
     6241 aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc ttagctgtta
     6301 cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga taaagctcct
     6361 taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag aggactaatt
     6421 gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc atcgatcgtt
     6481 tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc aaaaacagat
     6541 gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc aatcgcaact
     6601 aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac accagagtcg
     6661 aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac ttcaaaggac
     6721 gcaggacctc catctaattg acgtccacca tgattggata cccagatacc atctgcccct
     6781 gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat aactggaaga
     6841 tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt tgcagctgca
     6901 taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc gctatacgca
     6961 gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc ttcacggtaa
     7021 ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc tactgctttg
     7081 ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa aaactgcggt
     7141 gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt gctgatggaa
     7201 aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt ttcatgtgcc
     7261 aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat tccgaaaaca
     7321 gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat tttctttgta
     7381 ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc gcgaatatag
     7441 ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc aatgtttaca
     7501 atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc ttctttcatc
     7561 ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat ttactctcag
     7621 atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta cttcttggtt
     7681 ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct ctatttcatt
     7741 tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta ctgttccatc
     7801 actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg ttataggaat
     7861 attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag gcccctttgc
     7921 taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat
     7981 tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg
     8041 tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta tgccgtagtt
     8101 ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact cgttattttt
     8161 attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca aatctcctgt
     8221 tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg cttgctgatt
     8281 tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt agccgtggaa
     8341 aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg ttttttcttt
     8401 atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg caaacaaact
     8461 gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg gttctgtcat
     8521 acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca tatgatcgac
     8581 atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt tctgtactgg
     8641 tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg tcaacttctg
     8701 ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact tcgccaactt
     8761 ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata tgattgactt
     8821 cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca
     8881 caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt tcaacaagta
     8941 cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg acttcagttt
     9001 ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata ctttgcgcac
     9061 gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc aagacatacc
     9121 agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata aaaaaacctc
     9181 agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt gtggctagcg
     9241 agttatttta aaatccagcc aaataagctt tggatggctg tatccattac gtaaaacatc
     9301 aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag ttgttttctg
     9361 cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt catgagacaa
     9421 cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta caatatttac
     9481 aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat tttgtgtagt
     9541 tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt tacttctgcc
     9601 cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt tacttgtcaa
     9661 tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa tttgaaatta
     9721 agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt atataagaaa
     9781 aaagatctga caaccgttac agagactagt acattagaag aagcattaaa aattttagaa
     9841 acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt ccgtggaaac
     9901 atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc
     9961 actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt cttcaaagta
    10021 ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa tcatttttat
    10081 ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag cgtagaacaa
    10141 ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc tgctattgca
    10201 aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat cgaaaaagac
    10261 gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa
    10321 acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga aaatttgcaa
    10381 aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt tcttttttcg
    10441 ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg
    10501 gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa
    10561 atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga
    10621 agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg
    10681 tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac
    10741 caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa
    10801 agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag
    10861 gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta
    10921 cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt
    10981 tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac
    11041 ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta
    11101 aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc
    11161 ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat
    11221 aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga
    11281 agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg
    11341 cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg
    11401 ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat
    11461 tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata
    11521 tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat
    11581 ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa
    11641 aataagcata ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt
    11701 gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc
    11761 gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat
    11821 actttctatc agatggaagt agaattccca gttgcttata ttccagcagg ttctggaaat
    11881 gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta
    11941 gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa
    12001 aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca
    12061 aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta
    12121 ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga
    12181 ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt
    12241 ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc
    12301 gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa
    12361 aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca
    12421 acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc
    12481 acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta
    12541 catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt
    12601 tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta
    12661 cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg
    12721 aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta
    12781 ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag
    12841 tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg
    12901 atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg
    12961 atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag
    13021 gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa
    13081 ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg
    13141 tttcgtttac tgatctcttt gcgattaaac gctagattga tgacgctgaa accgctaaaa
    13201 atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc
    13261 atccgctgac tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt
    13321 gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc
    13381 aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt
    13441 atcttgatcg tcgccatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt
    13501 aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca
    13561 tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc
    13621 gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca
    13681 atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc
    13741 gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct
    13801 ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt
    13861 ggtataaaaa ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa
    13921 gttgacgaga tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg
    13981 ttccgacccc atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct
    14041 atccttttgc accagattca tttttggtat caaattacca accaatgtac ctaacaaaat
    14101 cgcacacaac gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc
    14161 tacttgatag ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt
    14221 aaagcttgct gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa
    14281 gcgaatacca tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat
    14341 cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat
    14401 attacgacct gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg
    14461 cgcgagatca acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa
    14521 aaattgatcc atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag
    14581 cgaaacagat gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc
    14641 attcgcttct acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac
    14701 ttgttctaag actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat
    14761 tccagtatta atcgtttcaa gattgccacc ttgatcaatc tcgccaaagg ccgccataaa
    14821 catcaatcct aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag
    14881 cagtattttt gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc
    14941 tcaaatcctt gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta
    15001 tttttccgta actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat
    15061 ttagttcagt agttgttact tggagctgac cttttttata agcgattcca tcagaagcta
    15121 ttgttcgctg aatcgctgac aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac
    15181 gatccgccgt ccgtaccata tcttttagag attctttggt tcctttagca atcacctgtc
    15241 cttgatccat gatcacgatc tggtcgcaaa gaatttctac ttcttccatg taatgtgttg
    15301 tatagacaat ggttgctcct tgtcgattca gctccttgat actttctaaa attttattcc
    15361 gactttgggg gtctacagct actgtcggct catctaaaaa aattaaccgc ggcttgtggg
    15421 caatcccaca tgcgatgttc aaccgacgtt tcaagccacc actaagttct cctggacgaa
    15481 acgaaacata tttttctaat cccactaatt gaatcacctc ttcaatcaag gctttccttt
    15541 tctctttttc ggaaacatat aaaccgcaaa aataatcgat attttcttgc acacttagtt
    15601 cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat tgccttgaca gcataatttt
    15661 caggagtcat tgttgtcccg aacaatttaa tggttccttt gtcataattt aacaaagaaa
    15721 gcatacagtt gatggcagtc gatttcccac tgccgtttgg acctaacaga cctaagactt
    15781 ctccttcttg tacttgcaga tcaaaatgat tcaatgctac aaaatctcca tagcgtttga
    15841 ctaatttatc aatttcgata atcatcatga aaaactcctt ccaaatttct tgttgatctg
    15901 actataataa agtgcaagaa aagaaactag tgacgattgg ctaaaccaca catgacattt
    15961 gtcacaatga aggagtctgc tatgcataaa tctcggctac tcaacgaaac tagctttcta
    16021 ctggttatct cattgctttt gctggtaagt caaggattcg ttgctatgca agttatctat
    16081 ttattgatcg ccgttttatt cactggtgtc agttatttac tgcctacaaa gccaaattgg
    16141 attttccaag ttattctatt tcttttatgt gcctttttac ctgatcttgt cctgtttctg
    16201 ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc ttgattactt gtttttgtta
    16261 ggttttttgt tgatttcgac ggttggtaat catcccccca tcaaggtcgc ctttgtcgta
    16321 attttgctgt ttttttcctt ttacttacga aaaaaagatc aacgaacgac cgatcttgag
    16381 aaaaagtaca ttgtctccaa agatactcat tgggaaaaac aacaagagtt atcggaacaa
    16441 aataaagcat tggccctgtc acaagaaaca caattagcct tgcaaattgt ggaagaacgg
    16501 aaccgaattg cccgagacat ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta
    16561 caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc attagaaaat
    16621 ttaagtgcca ccgttcacac tgggatggat cggattcgtc aaagtgtcca tgacctccat
    16681 gagacatcga tctcttttca acactcctta gagtttctga tcgctgattt tccttcttct
    16741 gttacgattg aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta
    16801 atggtcatca aagaagcctt aaccaacagt atcaaacata gtcatgccaa aaccgttgtt
    16861 ttacattttc gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga tggcaccagc
    16921 ccacaaaaaa attctgctcc cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa
    16981 caaaagggac aagtccatat tttccaagaa aaccagcaat ttcagttaac gatcatttta
    17041 ccaagggagg gacaccaatg ataacagtag tcattattga tgatgatcca tttgtgacga
    17101 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    17161 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
    17221 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
    17281 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
    17341 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
    17401 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
    17461 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
    17521 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
    17581 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
    17641 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
    17701 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
    17761 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
    17821 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
    17881 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
    17941 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
    18001 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
    18061 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
    18121 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
    18181 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
    18241 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
    18301 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
    18361 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
    18421 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
    18481 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
    18541 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
    18601 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
    18661 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
    18721 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
    18781 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
    18841 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
    18901 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
    18961 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
    19021 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
    19081 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
    19141 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
    19201 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
    19261 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
    19321 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
    19381 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
    19441 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
    19501 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
    19561 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
    19621 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
    19681 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
    19741 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
    19801 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
    19861 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
    19921 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
    19981 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
    20041 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
    20101 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
    20161 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
    20221 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
    20281 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
    20341 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
    20401 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
    20461 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
    20521 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
    20581 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
    20641 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
    20701 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
    20761 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
    20821 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
    20881 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
    20941 cgaataaaag tgggccatct ttatgtcggg caaccgcatc tgctacaaca gtttcgatac
    21001 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
    21061 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
    21121 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
    21181 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
    21241 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
    21301 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
    21361 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
    21421 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    21481 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    21541 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    21601 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    21661 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    21721 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    21781 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    21841 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    21901 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    21961 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    22021 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    22081 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    22141 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    22201 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    22261 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    22321 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    22381 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    22441 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    22501 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    22561 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    22621 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    22681 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    22741 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    22801 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    22861 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    22921 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    22981 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    23041 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    23101 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    23161 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    23221 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    23281 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    23341 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    23401 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    23461 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    23521 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    23581 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    23641 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    23701 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    23761 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    23821 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    23881 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    23941 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    24001 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    24061 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    24121 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    24181 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    24241 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    24301 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    24361 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    24421 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    24481 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    24541 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    24601 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    24661 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    24721 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    24781 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    24841 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    24901 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    24961 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    25021 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    25081 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    25141 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    25201 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    25261 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    25321 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    25381 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    25441 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    25501 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    25561 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    25621 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    25681 aatccatgct ttgcaaaaag catcaagcca gatgtatccc gatctagtcg cgtcaccaca
    25741 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    25801 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    25861 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    25921 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    25981 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    26041 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    26101 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    26161 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    26221 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    26281 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    26341 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    26401 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    26461 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    26521 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    26581 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    26641 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    26701 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    26761 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    26821 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    26881 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    26941 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    27001 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    27061 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    27121 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    27181 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    27241 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    27301 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    27361 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    27421 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    27481 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    27541 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    27601 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    27661 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    27721 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    27781 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    27841 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    27901 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    27961 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    28021 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    28081 taatccaaac cggaaagctt acaacaaaac gagctgaatt ccctattgaa gaaggacttt
    28141 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
    28201 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
    28261 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
    28321 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
    28381 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
    28441 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
    28501 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
    28561 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
    28621 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
    28681 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
    28741 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
    28801 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
    28861 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
    28921 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
    28981 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
    29041 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
    29101 a
//
LOCUS       DACEDM010000039        28030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEDM010000039 DACEDM010000000
VERSION     DACEDM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.039"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IWO50_001512"
     CDS             185..1183
                     /locus_tag="IWO50_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0358639.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IWO50_001513"
     CDS             1295..1804
                     /locus_tag="IWO50_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0358640.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IWO50_001514"
     CDS             complement(1834..2493)
                     /locus_tag="IWO50_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0358641.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IWO50_001515"
     CDS             complement(2495..3259)
                     /locus_tag="IWO50_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0358642.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IWO50_001516"
     CDS             complement(3267..3944)
                     /locus_tag="IWO50_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0358643.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IWO50_001517"
     CDS             complement(3981..5366)
                     /locus_tag="IWO50_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0358644.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IWO50_001518"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWO50_001518"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWO50_001519"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWO50_001519"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0358645.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IWO50_001520"
     CDS             6305..7162
                     /locus_tag="IWO50_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0358646.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IWO50_001521"
     CDS             complement(7243..7578)
                     /locus_tag="IWO50_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358647.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IWO50_001522"
     CDS             complement(7645..9015)
                     /locus_tag="IWO50_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0358648.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IWO50_001523"
     CDS             complement(9093..9629)
                     /locus_tag="IWO50_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0358649.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="IWO50_001524"
     CDS             9938..11419
                     /locus_tag="IWO50_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0358650.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="IWO50_001525"
     CDS             complement(11522..12229)
                     /locus_tag="IWO50_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0358651.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="IWO50_001526"
     CDS             complement(12308..12646)
                     /locus_tag="IWO50_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0358652.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="IWO50_001527"
     CDS             complement(12757..13719)
                     /locus_tag="IWO50_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0358653.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="IWO50_001528"
     CDS             complement(13734..15065)
                     /locus_tag="IWO50_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0358654.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="IWO50_001529"
     CDS             complement(15073..15753)
                     /locus_tag="IWO50_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0358655.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="IWO50_001530"
     CDS             complement(15972..16451)
                     /locus_tag="IWO50_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0358656.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16578..18257)
                     /locus_tag="IWO50_001531"
     CDS             complement(16578..18257)
                     /locus_tag="IWO50_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0358657.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18805..20307
                     /locus_tag="IWO50_001532"
     CDS             18805..20307
                     /locus_tag="IWO50_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358658.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20320..20982
                     /locus_tag="IWO50_001533"
     CDS             20320..20982
                     /locus_tag="IWO50_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358659.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(21083..21286)
                     /locus_tag="IWO50_001534"
     CDS             complement(21083..21286)
                     /locus_tag="IWO50_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0358660.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="IWO50_001535"
     CDS             complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="IWO50_001535"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0358661.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="IWO50_001536"
     CDS             complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="IWO50_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0358662.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(25250..25864)
                     /locus_tag="IWO50_001537"
     CDS             complement(25250..25864)
                     /locus_tag="IWO50_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0358663.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(26470..26847)
                     /locus_tag="IWO50_001538"
     CDS             complement(26470..26847)
                     /locus_tag="IWO50_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358664.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(27147..28028)
                     /gene="galU"
                     /locus_tag="IWO50_001539"
     CDS             complement(27147..28028)
                     /gene="galU"
                     /locus_tag="IWO50_001539"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0358665.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         8325 a         5875 c         4685 g         9145 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag cgccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctatcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc
    16621 aagatcactg ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg
    16681 tcttgcttta atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt
    16741 tcaatcatat tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc
    16801 cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc
    16861 gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata
    16921 tcacctatgc ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc
    16981 atacgcttgt tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat
    17041 ggcatcccta ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga
    17101 actggcacaa aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg
    17161 gcatggccag aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa
    17221 atgatatctt ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata
    17281 taaaccaaat ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc
    17341 agagacttga tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca
    17401 tagtttttca tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct
    17461 aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt
    17521 tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa
    17581 gcgacgatga tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg
    17641 gcttcagaag ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag
    17701 actccttctt tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc
    17761 gtttggtcaa atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg
    17821 atccccactg aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca
    17881 aagtctattt ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca
    17941 ttacgactga cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca
    18001 gaaatttttg acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa
    18061 aagacaccag ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg
    18121 tcgatcccca acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc
    18181 tcgcccactt ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata
    18241 attttaatgg tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga
    18301 ttttcttctt tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt
    18361 ctcaacttat ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat
    18421 gggcatttta ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc
    18481 cagaatactt atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc
    18541 gctagtatca gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg
    18601 atttcttttg ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat
    18661 tttaccacat tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga
    18721 tggtgtgcaa acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg
    18781 catctactag gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa
    18841 gtaaaatggg gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg
    18901 ctagctggcc tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa
    18961 gttggagcaa cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa
    19021 acttcttcaa gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca
    19081 ccagtttcct taacggacgc ggaaaaaata gctgaattag ataatgtaaa gagttattcc
    19141 tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat
    19201 tcaagtgaag acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc
    19261 gggaatccgc aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa
    19321 aatagtaatt tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc
    19381 gacaaaggca caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca
    19441 gtcggagata ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg
    19501 atcaaaggaa tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc
    19561 atgaatccgt cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca
    19621 tctgacgata acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat
    19681 gagtttctca aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat
    19741 gattccatgt atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat
    19801 gttgttttac tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc
    19861 attcgggagc gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa
    19921 atcattctcc aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct
    19981 tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca
    20041 gaaaccacga cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa
    20101 gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct
    20161 gatcaagtca gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga
    20221 gtagcatttg gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta
    20281 aacccaagaa aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg
    20341 ggattttggt atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa
    20401 gctggaaaaa tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg
    20461 cttatttctg gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg
    20521 aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac
    20581 aacttgcttc cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag
    20641 accaagcaag agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat
    20701 gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct
    20761 atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat
    20821 ctagatgaaa caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa
    20881 aatcgctgta ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc
    20941 tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca
    21001 aataaaaaat ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga
    21061 ttcaattagt aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga
    21121 cttcgtcgcc taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc
    21181 cttctgtttc tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga
    21241 ctactttatc tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc
    21301 ttagtttgtt tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa
    21361 cgctcgtttt ctaaaaaaga tagttagagc tgttgattaa aaactttttt cgaaagtccc
    21421 ccatactttg acttcttttt cttggaccat tatttctcct ttagcgataa ctgtatcaat
    21481 atcaagcgtt tctttatcca agatacacag atctgcatca gcaccaattt cgatttttcc
    21541 ttttttatcc aatttcaata tacgtgctgg attactggtt atagctcgta aggcgatttc
    21601 caaagggata ctttcttttt gcacagcttc tttgatccct actagcaatg ccttggcact
    21661 tcctactcct aaccctagga aatgattgtt ttcatcaaaa taaggcaagc ttccttgtcc
    21721 atctgaggtc atcgtgaaat tatctaaaga aacatcttcc tcgatcaatc gtttcagtgc
    21781 tttgctgaag cgaacttcac catctgtctt ctcccaaaaa tcaggatctt cgctagcagt
    21841 gaagtcaatc gtgccaccac gtttggcaaa tgccacgcaa gcttcaaata attctggtgt
    21901 gcgatttgca tgagtaggca aaaattgtgt gatcggaatt tctgtttctt ctacgattcg
    21961 tgtcaatagc tctagctttc tttttcctcc gcctaagtgg acattgatga tcccagcttt
    22021 tccggataac atgcctccta cacgagcatc tgctactgca cgagcgaact cttcaaaact
    22081 tggctgagaa gaacggtgat cggatactgc gatttctccg attccgataa ttttatcaat
    22141 tgtcaaaaag tctttgatga tagaatctgt tactgttttt accggcaatc ggtacgaacc
    22201 ttcgtataca taagtggaga ttccttctgc ttccagacct ttcgctttgc tgatcaacgc
    22261 agtcatatct ctaccttctc cgtctgttcc caaacaaccg acaactgtcg ttacaccagc
    22321 tgtagtcaat tggctcaatg tgacttctgg cgttcgattt tgataaccgc cttctcctcc
    22381 tccacctaaa atatggaaat gacagtcaat gaaacccggg gttagtactt ttccctcacc
    22441 tgaaagttct tctacttcca ctccttcgta gccgccttct agctgttctt ctacagccaa
    22501 aatcttccca ccggcaatca gtacatccat ttttcctaag taatctggtg caaacacttc
    22561 aatttgtcga atgattttca tacatttttc ctctttcttt aagtataact gatgatagga
    22621 tcttaaaaaa taatccaaac gataggatct ttactccttt gattttaccg taaaatgacg
    22681 gttcttggag tccttatcta ctgtttggat tactttttta tttacaggtc tccagtacta
    22741 ctgcagacct attgcaatgg caatcatgat gaaaatagtt cctaaaacaa acaggaatcc
    22801 ttgcatcttg atttggaatc ttgcccattt tgcccattcg atccgtgcaa cacctagcac
    22861 accaatcaag ctggctgata cgggtgtaaa agcgtccata aaacctgaac caagctgata
    22921 agcaagaact gctacttgtc tggatacacc tactaaatct gccagtggag ccatgattgg
    22981 catagtcaaa gctgcttgtc ctgaatttga tgtaacaacc aagttgaaca aactttggaa
    23041 gatgtacatc gcccaagcac caatgacagc tggaacaccg gtcaacgccg tcccgatccc
    23101 atgcaaaatc gtattcaaag ctgaaggaac agatgcatca gatcctccca agacaagaag
    23161 aatccctttg gccataccta ctacgattgc tgtaccagct aaatcagctg caccagattg
    23221 gaaagaagaa gcgatgtcat tgactttcat tccatctaat ttgaaaagaa cagcaatgat
    23281 tccagcaact aatcccatca caaagaattg agaagcgatt tctggaatgt aatagccttt
    23341 ttgagtcacg ccccaaacga tccagacaag gacaactaac atctctaata agatcaattt
    23401 atgcccaagc atgaattttt tattttcatc agatgtcttt ttgatatgag aacggaaata
    23461 atcatctgat ttgtaagtca acgaactttc tggtttttta cggatttttt ctgcatatat
    23521 catcatataa ccagcggcta atgcagtaac aactacccac ataatcaatc ggaatgtcgc
    23581 accagacaac acggggattc cagcaatccc ttgagccacc gctacgctaa acgggctcat
    23641 ccaagaggtc gcatttccga cttgagatgc gacataagtc acggttactg ctacgatcga
    23701 gtcatatcct aacgcaatga caaatggaac catcaccatt gagaatggaa ttacctcttc
    23761 tgccatcccg aatgtagcgc caccgaatga aaaagcaaag aataatagcg gtaaagctaa
    23821 acgctccagt cccttagttt tactgataaa cgcatagata cctgcgtcga tcgctccggt
    23881 acgcataata ataccaaaag cacctccaac aacaaggatc aatgcagcga tccctacagc
    23941 agatccatac ttatctccag atactagtcc ttcaaaaaca aaatttaaga aaccaaagcc
    24001 gccaaaatca tctgttcccc atattttggc tgttttatgc aattttttag aagtatcata
    24061 gatattttcg ccgtactgat cgtaaagcac atcatctgtc aaggagattt catctagttt
    24121 ttcctgcgta agattttttt ctccgtcagc aatgactttt tccaatcctt tttccggaac
    24181 atccagtttt tcacgttctg ctggatgatc ttgcagttct tctaattgat caaacacata
    24241 cgacttatcc agctcgtaag cataacggaa ggaatcttga cgtaaaacag tacgagtact
    24301 tgtttcgcca tttgcatctt tgtattcaat atcttgcgtg ctgaactttc ctgctggaac
    24361 gacaaaagtc agtatccatg caaagatgac tacaccaaaa ataatgacat acgtatgggg
    24421 tgtcgataat ttagtgaagt cgcgtttcgg ttttcctgaa gaatttgttt tcttcaaatt
    24481 tatttcttcc tttctttttt cagtaatttt gatgaataaa aaatggataa tcaaatgaat
    24541 tatacagtaa aaatgacatt tccacatgaa taatttatta gaaaatcact tttttctatt
    24601 ttgttttttt tctcctatgt atgattattt ttttctcttt ccatttatat taagtatttt
    24661 atttctctgt ttttccttaa aattcttttt ttatttttag attagagaaa atctcgtcct
    24721 gtttttttca ttttacaatc attgaaaaat tgaatttcat tgcaaaaaaa ataataaaca
    24781 cttataaata tccattatct atccttcaat ttttgtgtat tttatttcta ttagaaaaag
    24841 gtatcctgtt ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat
    24901 gtactataat tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga
    24961 gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt tttatttttc
    25021 tgacactaca tattcttata actcatttct cataactgtg aaacaactta taaaaataac
    25081 aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa
    25141 aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt
    25201 tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac
    25261 ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg
    25321 ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat
    25381 catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac
    25441 tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat
    25501 gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg
    25561 aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg
    25621 caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa
    25681 gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata
    25741 tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca
    25801 gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac
    25861 tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag
    25921 gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca
    25981 caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca
    26041 gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt
    26101 aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg
    26161 gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt
    26221 tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa
    26281 acattggtat gatttatata tatataaata acataaaaaa aggaatggct tatcccgata
    26341 caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca
    26401 attaaataaa tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc
    26461 tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga
    26521 aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat
    26581 ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc
    26641 taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat
    26701 cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag
    26761 tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga
    26821 ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat aattaaaaaa
    26881 ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca
    26941 ttttctttta tacaacttaa aaaaaaatta aaccattagc agaatatttt ctgactctat
    27001 tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa
    27061 ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc
    27121 attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg
    27181 ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt
    27241 gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca
    27301 tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct
    27361 gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg
    27421 ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact
    27481 ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca
    27541 ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc
    27601 aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac
    27661 ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga
    27721 gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct
    27781 gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca
    27841 atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct
    27901 tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg
    27961 gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg
    28021 accttcactt
//
LOCUS       DACEDM010000040        26894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEDM010000040 DACEDM010000000
VERSION     DACEDM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.040"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWO50_001540"
     CDS             591..1280
                     /locus_tag="IWO50_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0358666.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWO50_001541"
     CDS             1267..2475
                     /locus_tag="IWO50_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0358667.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWO50_001542"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWO50_001542"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0358668.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWO50_001543"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWO50_001543"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0358669.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWO50_001544"
     CDS             complement(6578..7273)
                     /locus_tag="IWO50_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0358670.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWO50_001545"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWO50_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0358671.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWO50_001546"
     CDS             complement(8472..8663)
                     /locus_tag="IWO50_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0358672.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWO50_001547"
     CDS             complement(8684..9574)
                     /locus_tag="IWO50_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0358673.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWO50_001548"
     CDS             complement(9561..10328)
                     /locus_tag="IWO50_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0358674.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWO50_001549"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWO50_001549"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0358675.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWO50_001550"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWO50_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0358676.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWO50_001551"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWO50_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0358677.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWO50_001552"
     CDS             14944..15282
                     /locus_tag="IWO50_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358678.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWO50_001553"
     CDS             complement(15450..16208)
                     /locus_tag="IWO50_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0358679.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWO50_001554"
     CDS             complement(16226..17044)
                     /locus_tag="IWO50_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0358680.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWO50_001555"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWO50_001555"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0358681.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWO50_001556"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWO50_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0358682.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWO50_001557"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWO50_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0358683.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWO50_001558"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWO50_001558"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0358684.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWO50_001559"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWO50_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0358685.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWO50_001560"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWO50_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0358686.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWO50_001561"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWO50_001561"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0358687.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWO50_001562"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWO50_001562"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0358688.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
BASE COUNT         7981 a         5345 c         5207 g         8361 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaa
//
LOCUS       DACEDM010000041        26505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEDM010000041 DACEDM010000000
VERSION     DACEDM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.041"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWO50_001563"
     CDS             complement(99..1229)
                     /locus_tag="IWO50_001563"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0358689.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWO50_001564"
     CDS             complement(1878..3080)
                     /locus_tag="IWO50_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0358690.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWO50_001565"
     CDS             complement(3140..3343)
                     /locus_tag="IWO50_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0358691.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWO50_001566"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWO50_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWO50_001567"
     CDS             4880..5692
                     /locus_tag="IWO50_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0358692.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6913)
                     /locus_tag="IWO50_001568"
     CDS             complement(6716..6913)
                     /locus_tag="IWO50_001568"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358693.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IWO50_001569"
     CDS             complement(7059..7937)
                     /locus_tag="IWO50_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0358694.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IWO50_001570"
     CDS             complement(7976..9094)
                     /locus_tag="IWO50_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0358695.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IWO50_001571"
     CDS             complement(9091..11190)
                     /locus_tag="IWO50_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0358696.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IWO50_001572"
     CDS             complement(11241..12062)
                     /locus_tag="IWO50_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0358697.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IWO50_001573"
     CDS             complement(12059..12868)
                     /locus_tag="IWO50_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0358698.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IWO50_001574"
     CDS             complement(12889..13383)
                     /locus_tag="IWO50_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0358699.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IWO50_001575"
     CDS             complement(13395..13883)
                     /locus_tag="IWO50_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0358700.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IWO50_001576"
     CDS             complement(13960..16656)
                     /locus_tag="IWO50_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0358701.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IWO50_001577"
     CDS             complement(17084..18157)
                     /locus_tag="IWO50_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0358702.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IWO50_001578"
     CDS             complement(18154..18981)
                     /locus_tag="IWO50_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358703.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IWO50_001579"
     CDS             complement(18982..19788)
                     /locus_tag="IWO50_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358704.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IWO50_001580"
     CDS             complement(19800..20885)
                     /locus_tag="IWO50_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358705.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IWO50_001581"
     CDS             complement(20902..21444)
                     /locus_tag="IWO50_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0358706.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IWO50_001582"
     CDS             21702..22691
                     /locus_tag="IWO50_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0358707.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IWO50_001583"
     CDS             22688..23425
                     /locus_tag="IWO50_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0358708.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IWO50_001584"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IWO50_001584"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0358709.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IWO50_001585"
     CDS             23989..24621
                     /locus_tag="IWO50_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0358710.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IWO50_001586"
     CDS             24706..25353
                     /locus_tag="IWO50_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0358711.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IWO50_001587"
     CDS             25358..26341
                     /locus_tag="IWO50_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0358712.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8112 a         5595 c         4496 g         8302 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaag
//
LOCUS       DACEDM010000042        26461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEDM010000042 DACEDM010000000
VERSION     DACEDM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.042"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(316..738)
                     /locus_tag="IWO50_001588"
     CDS             complement(316..738)
                     /locus_tag="IWO50_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0358713.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(728..2902)
                     /locus_tag="IWO50_001589"
     CDS             complement(728..2902)
                     /locus_tag="IWO50_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAQ0358714.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2918..5260)
                     /locus_tag="IWO50_001590"
     CDS             complement(2918..5260)
                     /locus_tag="IWO50_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358715.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5282..7825)
                     /locus_tag="IWO50_001591"
     CDS             complement(5282..7825)
                     /locus_tag="IWO50_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAQ0358716.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7835..8233)
                     /locus_tag="IWO50_001592"
     CDS             complement(7835..8233)
                     /locus_tag="IWO50_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0358717.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8314..8598)
                     /locus_tag="IWO50_001593"
     CDS             complement(8314..8598)
                     /locus_tag="IWO50_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358718.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8622..9620)
                     /locus_tag="IWO50_001594"
     CDS             complement(8622..9620)
                     /locus_tag="IWO50_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0358719.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            complement(9613..9960)
                     /locus_tag="IWO50_001595"
     CDS             complement(9613..9960)
                     /locus_tag="IWO50_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358720.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            complement(9976..10626)
                     /locus_tag="IWO50_001596"
     CDS             complement(9976..10626)
                     /locus_tag="IWO50_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358721.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(10637..11809)
                     /locus_tag="IWO50_001597"
     CDS             complement(10637..11809)
                     /locus_tag="IWO50_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0358722.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(11828..12700)
                     /locus_tag="IWO50_001598"
     CDS             complement(11828..12700)
                     /locus_tag="IWO50_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0358723.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(12722..13642)
                     /locus_tag="IWO50_001599"
     CDS             complement(12722..13642)
                     /locus_tag="IWO50_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358724.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(13642..13911)
                     /locus_tag="IWO50_001600"
     CDS             complement(13642..13911)
                     /locus_tag="IWO50_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358725.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(13908..14288)
                     /locus_tag="IWO50_001601"
     CDS             complement(13908..14288)
                     /locus_tag="IWO50_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAQ0358726.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(14755..16218)
                     /locus_tag="IWO50_001602"
     CDS             complement(14755..16218)
                     /locus_tag="IWO50_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0358727.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(16282..19299)
                     /locus_tag="IWO50_001603"
     CDS             complement(16282..19299)
                     /locus_tag="IWO50_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0358728.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(19450..20289)
                     /locus_tag="IWO50_001604"
     CDS             complement(19450..20289)
                     /locus_tag="IWO50_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0358729.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            20434..20679
                     /locus_tag="IWO50_001605"
     CDS             20434..20679
                     /locus_tag="IWO50_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358730.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            20742..21164
                     /locus_tag="IWO50_001606"
     CDS             20742..21164
                     /locus_tag="IWO50_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358731.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(21258..21575)
                     /locus_tag="IWO50_001607"
     CDS             complement(21258..21575)
                     /locus_tag="IWO50_001607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358732.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(21602..21808)
                     /locus_tag="IWO50_001608"
     CDS             complement(21602..21808)
                     /locus_tag="IWO50_001608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358733.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(21789..21971)
                     /locus_tag="IWO50_001609"
     CDS             complement(21789..21971)
                     /locus_tag="IWO50_001609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358734.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(21985..22335)
                     /locus_tag="IWO50_001610"
     CDS             complement(21985..22335)
                     /locus_tag="IWO50_001610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358735.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(22361..22663)
                     /locus_tag="IWO50_001611"
     CDS             complement(22361..22663)
                     /locus_tag="IWO50_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358736.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(22666..22899)
                     /locus_tag="IWO50_001612"
     CDS             complement(22666..22899)
                     /locus_tag="IWO50_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358737.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(23885..24207)
                     /locus_tag="IWO50_001613"
                     /pseudo
     CDS             complement(23885..24207)
                     /locus_tag="IWO50_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24330..25445)
                     /locus_tag="IWO50_001614"
     CDS             complement(24330..25445)
                     /locus_tag="IWO50_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358738.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(25451..25849)
                     /locus_tag="IWO50_001615"
     CDS             complement(25451..25849)
                     /locus_tag="IWO50_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358739.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQYSFLIYGQDEV"
BASE COUNT         7254 a         5543 c         4112 g         9552 t
ORIGIN      
        1 tccccccact tattacatat aacctttaaa taatctctaa ataaccctca aataatcctt
       61 actaatactg aaacgtaaaa aaaataaacg gatttcaaac aaaatacgtt tattttttct
      121 attgatttta gctcaaaatc gattcaattc gagtgaaatt tgcttcaatt ttcaattggt
      181 ttttgttcaa attttgaaac atttttgctc aaaaaatgag tgaatttgtt tcaaaagaaa
      241 aacaaatacg atcgaaaaaa atttgaactc ataacaattt tttgtttttt tcaacgtatt
      301 ctctttcaat tttcactaaa aattaggaag aaattgattc aatgtctttt caaaaaagaa
      361 tgtttttctc tcaatttttt tgacgtttct atcatgaatc acgagaaaac ttgggacact
      421 tttaacatgg taacgttgta aaaaagtttg aagttcttct tgtcttggtt cttttcttgt
      481 ttcaattcga tatacattcc tagagaattt tttgtcgtat ctttccaaga aagattcaac
      541 ttcttggcat tttttacaat catttcttgc tagatacaca aaaaatgatt gattcgtttt
      601 ttgaagttct agtacttctt ctaaattact attgattagt tttccatttg attcttcgtt
      661 agtttttatc agtttaaaac aaccaaataa gaggaaacta acaataagta tcctaacaat
      721 aactttctta tttcgcatat tgtttctcca aatttggttt ccgaatatag acgactcgcg
      781 gtggttcctg gacgtcttca gctgttcgat acaccacaaa ttcgcctgtt ctcaatgctc
      841 gaatgcgatt cggattgaat ttaaagcggt ctacgtctcg tttcgtgcct ctttctcctg
      901 tccaatcaac ctttcccata tcagaataac ctgcttcttg tgtcgtcatt tccgttaatt
      961 caatgtcttc ttctgtcccc attaaatctg cccaataatc aatttctgtc gggtcatttg
     1021 tcttcccaat aaagaacgta ttcacattcc caatcaactg ttttcgaagg acaggatcaa
     1081 ttctatcaat gtccgcaagc gtttgagggg catacgccgc atgcacccca gcgcctcgtc
     1141 ccttattgac taagtctata aggttatcat ttagataaga agctggttcg tcaaatatta
     1201 ctaggacttt cgagcgattg gctgtttctc ctgcttgttt atacacttct gacgcaaaga
     1261 aattaatatc tcccacaagc atttgagcca atgtccgaat aaactcacta taaatcaatc
     1321 cgtttaacga gatataaaca atctttcctt ctttaacaac ttcttgaata tctaattctc
     1381 tttttcctcc agaagtatca aagagttcac caagctcact atccaataac atattgatat
     1441 tggtctgtaa attctcagaa gtcgcataca aataaaacgg gttcttctta tgttcgtaac
     1501 gaacaaacaa ccgtttaaac aacgtttgct cgatttctgt catgagagaa atgttttctt
     1561 ttaataatag ataatacgtt tctaagtcaa cggttttacg atcatgttca acgattcgct
     1621 cttcacttaa ctgttccaca tcggcaaaat cacgttctgt tttgtccatc gcttcattct
     1681 ttttcgtttt tttcttcgtg gttttcttct tttctacgac cactttttct ttaaataaat
     1741 ctggattttt caactgaata cgatccgcaa acaaatgtaa gacgtttcgt ggcaataaat
     1801 aatgttgtaa ccaaactaga tttctttcaa ttttatggct cttttggaac tcgtctaaaa
     1861 gctgaatcgt tacctgtaat aacgctttgg ctgctgtcga ataatacacg ctttctgttt
     1921 ctgctagatt ggttaattta tctcgaatgg caatatcatt gccatttttt acaggattat
     1981 aacgcaaatc gccaccgtcc acaaagactt caatcgggcg attgtatgcc tctgccactt
     2041 cttttaacgc ttgtaaagtc tctcctgctc ctttcccatc gactaagata acggggatac
     2101 gaaacttaca cgcatgttct gctagtaatt ggatcaccgt cgtttttcct gaacctgtag
     2161 tcgctggaat aatcacatgt tggttcaatt cactaaagtt catcacataa gggagcccat
     2221 actcatctgt tcctaataga atcactttgg ctaatgaagc cagttgttta ttccgcaaag
     2281 caacgtcttt tatgacaatt gctacctggt acgcttgctg atactctgct gtatattttt
     2341 gggactcctg aaatttattc ttccgacgtt ccttgtattc tttactattt agcaaagctg
     2401 tttgccgttc tttctttgaa acaacttttc ttttttcaat aaaagacgca tacgcaatca
     2461 aacaacttcc tgcacctgtc ccacctaaaa ggagggcaaa aatcgtttgt ccattaaact
     2521 gcaacgcatg agaaaacaag tttaatggaa ataataagta acacgacaaa taccctctag
     2581 gaagaacaaa aataaaacaa aatagattta atagcgtaaa acctaacgca aaaataaaaa
     2641 aaagagagct tcttcgtttt aagaagctct catactcaaa gtcataaata tgtgcacgtg
     2701 tctctttttt atctatcata aagacaatcg ctaaaggaat actgcctaca ataaaaaatg
     2761 gatatagaca aacacctaat aaagtgacga taaaataaag ttgttgaaac cgactttttg
     2821 gcgtttcttt ttcttgtttt cttttgcgat cgacagaaaa catttgctca aaatgtgtcg
     2881 gtcgttttgg tgcattcatc atactatcga tttcctccta tcgttttcgt tgtttcattt
     2941 ctcttcgata aatcggttgt ttttctcgtt ttcgtttcat ttttgcttga gttgctcgaa
     3001 actcactccg tgtcttcggt gcttgttttg gtgattttgt ttgtttcatg gttgagtatg
     3061 ctttagatac ggttgaaggc gctttggttt gtgctgttac tcgattgact gctgacatat
     3121 gttcccgaac cccctctgga cgtcgaacag atttcggtgt ggtagaacct gttgctcgtg
     3181 gtacattccc ttttcttagt ggtttttgta ccgcatgttt ttgtcctgca actgtccggg
     3241 ttttcatcga acttgttttt gttacgggag taagtgcctc cttccgacga cgacttgttt
     3301 gtccctgacg acgttttcga tcgttcaatt ctgttctagg cgtatgcttc tggccataag
     3361 acttgtgttt ttgctttgga ttcgctaatg acaccaccat tgcagaaccc tgattgcgat
     3421 acggactacg agacgcacct tttctacttc tttctcttag actaggattg ccttgcaatt
     3481 ctcgttgtaa taaatcggct gtttttcttg cctgtttctc cattgctgtt cgttgcatac
     3541 gttcttgact ttgctcttct gcttgtttcg cttgatcatg caagtctctt gctttttgtg
     3601 ccatacgttc catacgattt ggtgctttat gacgaggttg ttgtgcggat tcacgccgaa
     3661 aagcacttcg tgtggcttgt ttcgccgttt gtggcatacg aacgtctgag ccacccccac
     3721 gttttcgttg acgttcttgt ctcttccgtg cgttttcttt ttctttttgt ttctgtgctt
     3781 ttctcgcttc tttgcgttct ttcaaccgtt gtttggcttc ccttctcaat tttgcttctg
     3841 ccccaaatgg atttcccgca aaagctaacg ctcctcgaac acccattctt ccagtaaata
     3901 gactgcctaa aaagcctcgg aacaaataaa ttcccaacgg cgcaaacaaa taaacaatga
     3961 tcaagagaat tttttcccat ggatcgtctt gtgtactact taattggaac ccgtaactaa
     4021 gaaaagaaga aaaaaggata catgcaaaac ctgtcattcc ttttaaaaac gtcaaaatac
     4081 caaaaacttt ggcatagtta ccaaacacat tcattgtgac aagaaacaat cctaaaaaaa
     4141 gtaagaacgg caacatcggt actaacaata gttgcatagt tccaataatc aatcgaaaca
     4201 tacttaagac aaaataaaca acggtttgaa tcagattcac gacaaaataa aacaaggcaa
     4261 atgctgtatt tttccaacta ttgtaatatt gtatgttctt gtttttcagt gtatctgctt
     4321 catactctgt gactttgtca tgaattttct cccctatttt tgtacctaaa tcattgtcca
     4381 ataaaagatt gattcgatca tattcttttc cagcatattt gactttcttc tcacgaattg
     4441 tgtctacatt ggttgtgcca taatttaaca acagatatgg ttcataaaca ttactataaa
     4501 agacccgagc cgccatcaat ttgcctgccg tttccacttt tttatccgct gttacctctt
     4561 ctgtttcttt tgtttcataa tttgtttctt cttcaggtgt taacactgga ttgaccttca
     4621 cgaacgccgt ttccactgta gaatctaaag aaaaaagaac atcaaaaaga ttcttatttg
     4681 tcccagaatc ttttaatacg cttaacccag taaaaataag gatcgtcata agaaagacac
     4741 ggagtgcttg tttcattcga gcctctccag catatttgat tgccatcata attgccgaaa
     4801 aactgattcc aatagttcct gcaatactta acatattgtc cgcaattttt tgtgttaagt
     4861 tttgaatggg ttctcgaatg gcggaaataa tatctaattc aaagagaaat tcaaccattg
     4921 aagcattgaa ttctcctaac atttttacgc cactccaaac aaaatttttc atcactcctg
     4981 caacattgta cgatagcttt tttttaaggt tcattgactc atagtcattg gtcatcaatt
     5041 caaacgaatt ctttttgtaa tttttataca attgatccac atcagtcgtt acttctttta
     5101 cttctacttt atcgttggca ttctctcctt cagaacgatc ggtttcagtg acacccgcac
     5161 catcattcat ttgatcggca gaaacagtag ccccttgtag acagaaaaaa agacccccta
     5221 ataaaaggag cgtccaccat ttcagagatt tttttcgcaa aaaaaccacc tcttttttgt
     5281 tttatcgttg tttttcattt tctaacattg cttgaatttt ttcatcttga ttttcagtag
     5341 aagaaacagc caataaccaa ctatcaaaca acacatcaat ggcaatcggt tgatttcttc
     5401 ccaaatgatc ctggaataaa caggttcctg tttgtaattg ttccatcatt tctaaattgg
     5461 ctgaattcac ttccatatca aagaaggaca atagtttctc tcgtgcttct ttttgtttcg
     5521 gacgaaacgc aaatttgtaa gaaattaact cttttttatc ttcttgatcg taatctttaa
     5581 acgcttgcac aacaaggtaa atatccgtat tattcgcacg tccttttcgc aagctatcat
     5641 caatcaaata gcttccttgt gcggtatctt catacccttt tgcctcgtca taaataatcg
     5701 cagcgtcttc ttctttgttt gtagaaaaga tatacgtaaa cttcataatg acttccataa
     5761 ttgccattcc tgcaagttgt tcgctattta atcgacccga atcaatctct ttttgtgtcg
     5821 gaatgcgtat tccttgtgtc cctaacacgt tgatttgtga atcaaaacgt atcggcgtac
     5881 tgtgctcatt cccaatgaga atctttccta aaccaacgct atgccctaaa atcaatttag
     5941 ctagttcttt gtcacttttt cgaatttctt cgataacttg acttaacgaa tgcttacctg
     6001 gcatggtgta aactacatcg atggcgtctt ggatcaccgt tttcttgtgg ctcgctgttt
     6061 ctcgattggt atctacttct cctaacattc ctaacacatt tttagcggtt tgaattgcct
     6121 cttcttttgg taaaaacaat agaggatcaa gcatccctcg acagctttca tctgaagata
     6181 acgaaataaa attaattcgt tcatacatag ccacaaactc tggtatgtgt ccgaattttt
     6241 caagtgcctt taaaaagaac ttttgggttt catttttcgg atcaatataa agaatttttt
     6301 gacctaaaaa catcagccat aaaaaaatat attttacaag gaccgatttt cctttacctg
     6361 gtggaccaat aatcaacgta tttccattgg tatgttccgc gccttcaata cttttctttg
     6421 ttaaattggg ataataccat actagtttac tagatagcgc taacgcttct ttcgcagatt
     6481 taaacttttt tgcaataaca atccgtccta aaggaaatcc tttcttattt cctacttctt
     6541 tctttaagtc caagccataa tctgccacaa aacctgtcgt agcgacctgc tcgtaactac
     6601 gaaaacgttg cgtacttcca attaagcatt gattaaacaa cgttagctga tccgctaccg
     6661 atcgataaaa tttgaaatct gtagactcaa tcgcaaattc aatatctttg actcgttctt
     6721 ccaattcttc ttttgaagaa gcagatacaa ccagcgtgac ggtagaataa cacaatcgtt
     6781 tttctttgcg cttcagctta tcttttacat ccattaaccg ttctgtacca aaatttaata
     6841 catcgtctga atctaaaatg ccgtcaacgg tctcatattc tttttcttgt tcataaatcc
     6901 gtccagccat tttattaatt tttcgttcat cagattttga atgctcaaat cgaagtcgaa
     6961 tatgcgtatc aattggaaaa gataagctat cctgaagatt ttgaataaag ctactcccat
     7021 acatggctaa aggagcgtcc gtttgactta aaaacgtcga ataatgcgtt tgattcaact
     7081 gttcaatcgc aatgtatcct gcttcattcg aaatgacgcc ctcgcaaatt tccattggcg
     7141 taagctgtct ttcaggcaat ttttcttctg atcgatggaa aaaataataa taaatttgtc
     7201 caatctcttg ggcagataag cgctctatcg gcttataatt ggacaattcc gtatagagct
     7261 ccttttctaa tgtttcatac gttggtaaca cactacttat aggaactcgt tgtcccgtaa
     7321 tacgatagat aaacttttta aaaacatgct taagcaattg aaaatattcg atcggatcag
     7381 acactttcat ttctgtagtg aaacgcacaa acagatacgt tcgatactcg tttaattgca
     7441 cttcgtcttt taaaatcttt cctgcctgtt caaagtacac tttcccaaga tctgcgcatt
     7501 ctccctttac caaatctcga atggtttctt caatgtgcgt atctaggtta aattgacgtg
     7561 gaatattgat aaaatgatag tcatattcat catgtccaag tatgccttcg ccatcttgaa
     7621 gatagttctt gaaaaaatca atgtcattta aaggaaatac ttgggaagaa agttgatagc
     7681 ctacccacac atcatcattt ttggtcaaaa caaaatttcg gataatggcg tctatcggaa
     7741 attctaatcg aggaagcttc gtttttttct ttttgaactg ccaaatcccg ataataaaga
     7801 cgaccaaaag aaacaaaaac gccatttact cgactcactc cttccatttg tgtagggaat
     7861 acgtcacttt ttcatggaaa aacggcacat acatttcatg ttcaaagcgt tgtttccgtt
     7921 tctgataata ggacgttcgc ccaattaaat actcgaaaaa gtgtttattg tcccatttca
     7981 ttgaaaacaa tgtccaaacg agaacaccaa tcccaaaaat cataatcaac caggactttc
     8041 gaaacagtgt ctgcaagaaa ctaatttgat tcacatacgc aatcaaaaat aggatcaaaa
     8101 gaatgaagag aaatacccca cctatgacta attgttccat gcgaatgcca ttagaaatcc
     8161 agggaatttt ttgtccttta atcccgtaaa ttttgtgagg gtgcttaaac tcctttgtat
     8221 agttgtattc catgttcact ctacctcctt ttacaaaaaa agaagcgacc agaagttcta
     8281 atcttctgat cgcttttgca gtttgtcttt ttcttatgat aattgtatcc agggcacaat
     8341 catttgcgtt acccccacgt tctcttcact atccttaata aagataatgt tctttagcca
     8401 ttcaccaata tcaacggcat tcgataacaa taagacgagc attatcaaaa taaagacgcc
     8461 aataatggca aacgctgccc gcgcaaagcc ttgggtcgca taagcaccaa ccattagaac
     8521 aatcgcaacg attgccaata gccaaatgac ttgtccagat acatcatcaa ataacgattt
     8581 aagatcaaaa agtttcattt ccattctcct ctaagttcat tttattcatt taacgtagta
     8641 acaaaataac tatcgccact tgtttttact tccattgaaa agtttgattg gaactcgttt
     8701 cctttctcaa aatagaaacg atacttccct tgcaaaaagt agtttccttc tttatttttt
     8761 actgcatgat ccagttgtac attgatcaat tcgccgcctt ctaacccttt ttcgttagaa
     8821 atcaatgcca atttttcatc atttttgaca tataactcaa aaaaccgttt cacaaatgct
     8881 tccagcttga tttcttcgct ttcagtgacc gcttgcccag aagaataaaa tcgattttcc
     8941 tgatagttca cagaattggt attcttactc tcagaaagat tcaaaaattg aacgccatca
     9001 agtactttta aaacattgtt ttgataactg actggaaaag aaagttgact gcttttccaa
     9061 tgttctcctt ctgtaaaagt aacctcataa aataggcgat acattgcttg tgtcaaatcg
     9121 atttcttcta gtttaataaa gcgaacatct ttcgctactc gattggtagc cgtcgttaca
     9181 aagcctagat cagacgcttc caatccttgt gctaaataat gattgacgga agcctcccat
     9241 tcgtttcgtt tttctggcga ataagtaaat aacctttcaa taaaatgttg tccctcgtac
     9301 ttcaattgtt ccgtagaaga caaggtttgg ttgacttcag ttaacaatgt ttctggattg
     9361 acttgttttt tcactgctaa acgcgttaat gtatccactc gtccaaagtt taggaggagc
     9421 acgacaccca ttaacagaaa caaacaaaaa aaagcgcctg aaaatacact catggcactt
     9481 ttttgcgaaa attggatcat tcgatctgac ggttctgttt tctttttttg gaacaaaaaa
     9541 cgtttctttt gtttcttttc cttctgttgt ttcggtggct tagtttggaa cgcttgtgga
     9601 ttaaattttg cattactcat gagaaatgcc ataccgttct aacaataatt tcggtatttt
     9661 gtccacaatc actacttcgt ggacaaccac acttgccccc tccaaaaagg caagtgcttc
     9721 tttgtaggca ttttgactgt atttttttga taaagcatga caaatttctt gataaaatag
     9781 ttgactgcct accgaatacc gattattgtg tatccgccac aaaacgaaca cactgatttg
     9841 aatcgacggg cgtaaaaaag aaaaattctt tccataagaa aaagaacgtc cttcttgggt
     9901 ttcttttgct tgttctgttg gtacttcttg tacctctctt attgattctt gtctctccat
     9961 aaccttctcc ttttcttatt cttctaaaag ctgttgatta aagctacttc ccaaattttg
    10021 cataaacgtg attttctttg acgctggatc aaaggttacg tcaaaaatat tcatggcatt
    10081 attccaggaa ctatcaccaa ttttattgcg atacgacacc acaacgagcc cttggatagt
    10141 cgctttttgt gtgcttttta aataaacaga acatttctct aattcagaag acaccccgtt
    10201 tgaatcacca taggaaacgc cttctttgaa atattgcttc atcagtttgt cagaaagata
    10261 tggtgcacaa ttgttttttc gttcttgaaa agaatcaggc tcataattaa acagaacctc
    10321 gaacgcttct gtcactgttt tttgaaaggt ttgaatgttt cctttttctg ttgaatcatt
    10381 gtttttcaat tcttcatttg ctttttttaa cgtcgtgacc tcgctttcta cttgttgaag
    10441 ttgttctttg agtgacgtta caaggtcttc ctgtcctttc ttctcttctt ggaacttgtt
    10501 tatctccacc gtcagtttct cttcctttgc tgttagctgt agcttttcat gaataagaaa
    10561 aaatgtactg cctacaccaa ataagaacaa tccgcctaca caaacataaa taaatcgttt
    10621 cttcatttga cacctcttat ttctttatcg gtcgagcaaa acaaatatca ccaccattaa
    10681 gcaaataccc aaaggataaa ttgacttgcc cttcacctac accgttagta ttcattcccc
    10741 cttgttcgat tactttcgta gaaagtccat gccaatcttc cgctaaaaca gctgtgtgtc
    10801 catcatttcc cacaccagaa cctaagttca caatgataat atcgccagct tttgcctcgc
    10861 tctcaggtat ttcttgtaag tattgcttac ttcctcttgc gtcgctcgtc attgagccgg
    10921 taaaccaccc aacgttcgca ggcgttttat accctgcttt cgttaaaaca agccacacaa
    10981 agctcgaaca atcggcatag ccatttttat ctggattttc gacactacca aattgtgccc
    11041 gttgcggttg tgaataatga aaataaccaa tcagactttt tgctacgttt aatacattac
    11101 tatcggaaga actgcttgtt cctgttgttt ctagacaata atcggtcaag tctttacttc
    11161 ctgcgtttgc cccattttct aatgtaattg aagcgacggg atcactgtca ttctttccta
    11221 gttttgcgta ccattgattc gcaaaagtaa tacgattccc catcattggt atccctgcac
    11281 gctcaaattt cacttgccac tgttctgttg cttcctctac gctttttgct tgtaatgcaa
    11341 cttgtgaagc tttttctgtt gtgtttagtt catgaatcaa aaactcaact tgtacaccta
    11401 aatctgacca ctctttgttt cgctctttgg caaaattttt caaagcagtc gttcgccctg
    11461 ccgtccattg tgcaatccca tgcccagata atggatcgtt tggtcgttca atcgctgtgg
    11521 gactcatact cgactcttgt tgaaaattgc ctaacatacc cgctagaccc tctaatgtcg
    11581 tatttggaat atgtttttgt acttcctttg caatttgaag cccgtttgtc tccatgcctt
    11641 ggttcgttaa tgagatagta cttgcgacaa tttcggttgg actacagtcg atttcttcct
    11701 cttcagaacc actactaatc aaaagtatca taaacagaaa accaaacata catagtaata
    11761 gtgtgggtag acaaccgaac ccacaccata aaagaagttt tttcttcatg tttgatcctt
    11821 cattcgctta gttcattttt tgtgttgctt ttcttttgcg ccaaaaagca aaaccgcttc
    11881 ctgaagccaa taatccacca agtgctgtcg caacgagaga cacttcttcg ccagtcttag
    11941 gcaatacttt catttttcca tcagaccctt tcaccgcaat atttccgctt ggaagttgtt
    12001 cgaccttgtc tgaaatgggc gtcaagccat ttcctgattc tttataagcg acaccatcaa
    12061 cgacggagac aattttttct cctgaagctg tttcaattgg tgtacttggt gttggaatat
    12121 gggttaattc agacgcatta ttggtttcaa tttgaacatt ttgtccacct gtttgttggc
    12181 tgccaatact tccatctggt tttactacga cgtcctgtac tggaggcgta gtgttcgtcc
    12241 ccccattatt ggtaggtggt gtcgttggag gagtcgtatt tccttcattg cctcctggat
    12301 tcgacggctt ttctggtgtc tctgtattac caccatttcc tgggttttcc tcaacgggtg
    12361 gaacttcagg tgttccttcc cctccgccag tatctggatt ttctggtact tcaggcgttt
    12421 ctgtgcctcc acctgtatct ggattttctt ccgttggtgg aatctctggg gtttcaggag
    12481 tttctggtat ttcgggaatt tctggggttt cttctcctcc atctgttggt ggtaaaacag
    12541 gttcttcagg aacttcaggt gtttcttccg ttggtggaat aatttctgtc ggtggtacac
    12601 ttggatcacc tggtgacgta gtatctgcat acgcactggt tcctaccata cctagcaata
    12661 cagttgcact aaataaactt cctaaaatca tttttttcat gtgtttttcc tcttctctta
    12721 tttattgatt tatttcaaaa tcatctagtt cgggaagcgt ggaaactaga taattttttt
    12781 cctgttcttc caattgaaaa gacacttttt ttgtttcttg tgtctttttc tcggtttcca
    12841 atgtaatgac atacgcaatt tgccaatgct tgccttcttt cttcatttcc caggataaaa
    12901 tacttttgac tttttccttt tgaggactga tttgtttcgc aactttttcc gttagaaacg
    12961 attgaagctt tttcctatac cgttcttctg ccatttcacc cgaataataa ttcgtaataa
    13021 aataacgact aaatgtatca atttgtggtt gttttgtcgc aagttcttcc gcaaatgcaa
    13081 tgcgttcttg taactggtgc tcctttttcg ttagttgttc aatctctctt tgcgttgaaa
    13141 gttgcataat aaaaagacca caagatacaa cgacgataaa tccaaacaaa agccattgaa
    13201 aagacatttt tttcttctga agagagccgt tgctttgtgt aaacgctgtt tctacagaaa
    13261 gtggtgtttt cttttccttt ttgatttttg ttggtcgacg ttcgttcttt tggtctttct
    13321 ttacttgata aaacttcggt aattcggaat ttaaggtttg aataaaggtt tgtgcttctt
    13381 cacttaattg cccattttct gttaaagatt gttcaatcaa tgaaatcaaa tgaaactctt
    13441 ctactggaaa atcatacgag ccttcaaata ctggaatttc atggttttct gtgtagattt
    13501 ccaaggtaca ccagccaatt tccgctcgtg ccaatagctc ttcattttct cttttcaatt
    13561 gaataaatgc cttatctaat gcaatttttt tatctgttag taaagacatg ttatcaactt
    13621 ctgtaaatcg aatcatcatc attagtcaaa cacttcttcc accacttcta ccgcatacac
    13681 cacttgaata gggttattta aagaaaattt cacccgatat tcttgattgt tcaaactgac
    13741 gatatagcta tcctcatctt cagcataata ggtatattcg ttgcctttga cttgaaaata
    13801 tttttggatc gcttgtcttt gttgccattc gccaatcttt ggaaaatagg cgaaatgata
    13861 gccaaataaa acgcctatcc ctaataaaac aattcctgtt actataatca tctggtttcc
    13921 tcctgtacct gtttatcttc tattgactct tttgtcggta agcaataaag aaccagtaac
    13981 acaagagagc caatcgttgg gacaaaatag aaaaaaataa atgcccaatg aaaacctgcg
    14041 tctcgtaaac gtcgaacgct gagagaaaga gacgggacaa gaactacgaa acaatacaca
    14101 atcaatacgc ctatcaaaca accaattatt gtttcagaag cattcaacgc tcctgctagt
    14161 ccaatcgcta agcctaggcc aaacccaatg agggcattca ttaacgcaac gagccaatac
    14221 tcttttcttg tggctttccc tttgtaatta aagctattct gccaaaattt tttataattt
    14281 gtgatcatat ctttgtcctt tctaatgttt atttttgatt ctagtttctt ctgttttact
    14341 aacaatgaaa cttgccataa aaaaggtggc caatacacca ccagctagaa atccacttac
    14401 aaataacaaa attttttcct cctttctaaa aaatttcgcc ataaaaaggc aaagtatgaa
    14461 cacgataaat accatgtaca tactttgcct ttttatgcaa tatatgttaa ctctccaagc
    14521 aattactgat ttttaaattc tttataaata aaataaaaat cgtttgagta aagaaataag
    14581 tcaatgaaga cccaaacgat aaaaaatatt cctaatacta tatttaataa actacgttct
    14641 agatagatac gagatttcaa attaaaaaac gaaaatgaga gatacaaata tatcaaatat
    14701 ttaaaaagta accacatttt ctataccact ccttatcaga aacagcctta ttctttagaa
    14761 taagactgtt tctgggttga ttaatttgtc ttttgttaaa ctaccatcta aggcaagttc
    14821 taaatgtaaa tgtggtcctg tactatttcc tgttgaaccc acataaccaa taatttgtcc
    14881 ttgttcaact gtttgtccta cgttcacgca atattcctgt tggtgcgcat aaagagccac
    14941 acttccgtct tcatgctcaa ttgtgacata atttcctgac aaagtatgat acgaagcttc
    15001 tatgaccgtt cctgccttgc tcgcatagat tggttctcct tgattggctg ccatgtctaa
    15061 tcctctatga aattcttctc ctcgccaacc gaaggaacta gaaattgaat aattttttag
    15121 aggtaccata tagccttctt tgcttagttg tggaccagct acttctttat catatttgat
    15181 taagtcatag gtttcgatta acccatttaa ttttttatcg tagctagtgt ctgttgcgta
    15241 tcttcctgtt aaaaatttag tcgcttcctg ataggttttt gccttacttt tccaaacacc
    15301 atcataaaaa gaggtatttc caactaagcc ttcttttaac agtttggcat agtcttttaa
    15361 gctttcttca tagctctcat attgccgaaa agttgcttgg gtcgtataaa gtgtcccatt
    15421 gcctaaatct tcttgtgtcg caaaagagac gccctttccg ttatgtgtgc ctttgattcc
    15481 aaatagattg tagtttggtg cctgagccaa ttgactttgt ccgcttgcgg attctaaaat
    15541 ggcttgcgcg atcataacag aagcgtataa atcattttct tgtccaattt tacgagcaga
    15601 ttcaccgatt ttacggataa acgtttctac agacgcgtct ttttcaaaat gaatagtccc
    15661 ttcgttatct tgtgtgctgg aagggactaa ttcttgtatc ggtgcgcttg gtgcttggtg
    15721 gtagtttttt gtctcttctg aaccgattga ttgtgcctgc ggtacgtcat tcataggtac
    15781 agtcacactc tgttcaggtg ccttttgtgg gcgttccgtt tttgttggcg gttccgcttc
    15841 tgtttcggta cttggttttt gaggagtctg tgagctttca tcagaaggtt gctcatttgg
    15901 ttcttctgac ggttcctcct caggttgttg attgtcttct ggtttgtctg gttgattcgg
    15961 tgtttctggt tgttcttcag aacctgttgg tggagcgttc atttctggat tatctggtgt
    16021 atctattgta tcttcagtcg gtgtttctgg ttgattttct tctccagtgg aatttgttgt
    16081 cgtttcttca gtgatcgttg cagattgcga atttgtcagt tgattgttgc cttgttcatt
    16141 agccaaaacc gctggcgtat acccaaatac ttgacctact aataacaaag cagataaaca
    16201 tttactcgtt tttttcacaa gattgcctcc attcttcaat caatcatcta gagacagctg
    16261 attgctgtct ctagtcatac tttaattgct ttgtttcatt ttacgacgaa ccagaacagc
    16321 ccccacacca acaaaaagaa acagtcccaa tgcaattaac acatcgttca ctgtttctcc
    16381 tgtttttggt aagctttgtt ttggtgtgct cgtcggcgtt tgtttgacag gggttgtcgg
    16441 cgtttctttt ttaatgcctt ttgctgtcaa tgtttgtttt tcattcttca aatcaaagtg
    16501 acgtgcttct tcctctttat ggtctttatc cttatagaca atatgagttg cgaccaattt
    16561 ctccccgtct tttaacgtgc ctgctttgta gtcaaaggat actttaaact cttcagacgc
    16621 tttagaaact ttacgtgcag tttctaccgt accaacgact gtatcttttc catttttatc
    16681 aatcttatgg aattgagtca cccagtattt ggtttcccct accgtagatt ctggaaattc
    16741 ttgcgataca tgatcgatca actcttggtc tttgtctgga tcaaatgttt ggtcgccatt
    16801 cactgtaata aagcgtgttt cgatttttgg ttctttcgca gtcactgttt gatctttatt
    16861 ggttaaatca aaatgacgag cgaattcttc ttcctcttct ttgtcgttat agacaatgtg
    16921 cgttgcgaca atcgtgtcac cgtctgaaac catgtctgct ttgtagttga acgtcacatc
    16981 aaatgtttct ttttcatcgg tcacttttcg tgtttctttt accgtatcta atacagtgac
    17041 ttttccatcg gcagtaattt tatggaattg cgtcacccaa tatttctctg tcccaaccgc
    17101 agatttaggg aatacttggg acactttatc cactaattct tggtcttttt tcgggttgaa
    17161 atcttttcca cctgtcgttg tcacaaacaa agtagaaatt tctggttggt cttcttctgg
    17221 aaccacgatt tcttcgtttt tcacagtaat tggttgtact tctccgtctt tgaccgttac
    17281 taaaatcgtt tctgtttgtc ctgacgcttg cccataatgc tcagggaaca caatttccgt
    17341 aaatttatac gtaccgtctg gtaattttag tttttctggt tcttcttttg tttcatacac
    17401 aaaggacaca tttttattat gtgcagtcat accttcaatc aggaatttcg ctcctggtaa
    17461 ttgtagatga ttgattgaat cgactttatt gaagtctact tcacccgttt ttaacaagtt
    17521 gtcaatcaca tctaattcaa acgtaagcac aggcgtcgtg ttatcgccat aggccacaga
    17581 gaacgtggtt tgttcttcag aaggttgacg gtagttgtct ggcacatttt tttcaatcaa
    17641 ttggtatttg ttttgatcgc cattgtagaa ttttttgttt gccttccagt gtccgtcttt
    17701 gtcagttttt actgaatcca agacttctcc ttttttcaca aggactgttt ctttatctgg
    17761 cgcagtaata tctgttttgg cgataatatc aaattcgata ttttctgcag gaacggtatc
    17821 ataaacaggt ttgccttgat tccaggattc ttccgcatta tagacttctt tgactttctt
    17881 tccttctgga atgaactgtt gtaagtcatt ttttgcagtt aacgtcactt ctttgtttgg
    17941 ttcaatcgta atcgtatgag tttgtttgtc tagaatatac ccagctggtg ctttgacttc
    18001 tttgacatcc aatttatttt gaacatattt tccgtctttt tcatagaatg cagggatttc
    18061 ttttaaggcg atttcccctt tggcatccgt tgtatattgt tttgtttctc ccgtatcatg
    18121 acgtttgatt tcatacgtca catttggaac ttttttccct gtgacagcgt ctagtttggt
    18181 tagtttgaag tcgccatttt ttagaacttt aatagttagt ccagctctat taggatctgg
    18241 cacataaagc tttcctagat tttgagcaac aggatctaca taggcaaaac ttgaacctac
    18301 ccctctagaa tttggatcca gtgaagaaac agaaattcgt ccgtcatttg agttttgatt
    18361 tgcttttata aacaaactat ttcctgattt tcttacttga acgttagctg tgttttcaac
    18421 aaccgaccca tcccattgag aaaacacacc ttttgtatcc ttaacttcta cttcttcccc
    18481 tactttaacc gtcactgttt ggctatcaaa agaaggatct tcaagtaaat tgtttatttt
    18541 tctatctaca gtctgtttga aagcttcata attagattgt ccaatagttc cataattaac
    18601 aaatgaacca cctaaaactt cccaaatata tgtttgtgct aatacatagt tatccattga
    18661 accactagaa tagtagccaa aataaccaat taaagaaatc ttttttctaa tagcacttgt
    18721 cgtaattaaa ttttcgagtg gtgtcacatc atattgaata cccgcactcg tgattgcgcc
    18781 ttcttgaatg caaaaaacat cttctccgtc aatttgccag cgaaccatgt aatcatgagt
    18841 atacattgtt ccttcaggag taattcgagt catattcaca tctttacgac cattttcagt
    18901 caatgttaga ttggtagcac gtgtctgtgc attcttttcc aagattgctt gccattcttc
    18961 agattcttgc gccaatcgtt cttgttcaga aaaagaagtc gctttttgaa tatcttcaat
    19021 ggttataaag tatttctcat aaccttcagg gacctcttga gtcattacat tgggcggaga
    19081 aacatcttct gtcgtgtcct gcgtagttgt ctcttctgtt gtttcacttg tggtttcagt
    19141 actgatcgct tcttcactac tttttgattc ttcagtcttc attacttctt cagtagaatt
    19201 tgattcattt atcgtttctg caaatgccgt tacagatcct aacatgggat tgagaaatgt
    19261 aacgaaaatt gccaaccaag ttgccattct tttaaacata tttttttcgt tcctttcatt
    19321 ttatttttca gtcttctaaa gtaatttctt atggacttcg acaccgaaac atttttatcc
    19381 tcctcaaaac aaaaaaagac tagaacttat aattctagtc ttcatcaacc acttactttt
    19441 cttttatttt tagtagaaat ctacagtgtg tttttgtgac ccgtcactgt aaataataac
    19501 atccaccata aaacctttgt tgtaccactg cgaatttgga tcgctaacct ctgcattagc
    19561 ccaatcatag gcttcatcta cagtgttaaa tacctttcca ctattaccaa tctctccaac
    19621 tacaattgtt ggctcttcag gtgtcgttgt gccaccattt ccgccttgat cgcctccacc
    19681 aggattgctt ggttcttcag gcgttgttgt tcctccacct gtgcttgggt tagaaggagt
    19741 tgtttctcct ccagtatttc caccattacc tggttgtgta ggtgtttgag tagtgccacc
    19801 attgttttct ggtgtacttg gcgttgttgt accaccagtt gttcctgttt gatctccagc
    19861 tggtgttgtt ggtgtcgttg tatttggtgt cgtttgtgta tttgaagtcg ttccttgtcc
    19921 tactggttga ttcggatcaa ctttgtcttt ctcttgaatc actgtttgag ccacagtttc
    19981 acctgcgctg ttttgtacac ttgctacatt gccattaaat actaatttat ttcctgcata
    20041 gattaaatcc acatttgtaa tcttattcat gtccgctaat ttttggacag taatatttgt
    20101 tgcttgactg attgcgctta gggtatctcc ccagacaatt gtgtattcat tccctttaat
    20161 ttctgctttg atttgttccg ccgaacgagg tgtccaaact ttgttttctg ctgcttcagc
    20221 attcgttccc acagcaagac taactcctcc gactacgatt cctagtgcca atactttcaa
    20281 cattttcatt gtttttcgcc tccgatttga ataaatttat ttacaaaaac atagtaccaa
    20341 acttatgtct atcagtcgat atattttttg tagatttttt tattttttga tatactaact
    20401 ttggtactaa aataactaaa aggaatgtca cacatgcaac ttaataattt aaatgaaata
    20461 catgattggg ctcaaaataa tttacttaat cgtaaacaag ctgccaaaat cactggacaa
    20521 aactatgatg ctttttctag ctctttacgt agaaataata tcaaaccatt tctagaaata
    20581 ggcgacgaac ctggtccttc catcattcgc ttatatctaa gagaagatat tgaaaaatac
    20641 gcaaaacagc tcgaagaaaa aagacagaaa cgtcaatgag gcgttttctg tcttggattt
    20701 acaagtaaaa caataataaa cacttttaca acggaggttc tatgaataag aaatacattg
    20761 tttcagttgg ttcaatcgta ctgattgtac tcgctattat tgctttttct gtatccaaca
    20821 aaacagaaac acagccacaa aatacggttc aatcgactac tacaagtcaa tctagcgatc
    20881 attccgtcta tccttcacta aatagtttaa aagaaacgca tggacaagat ttaattgatc
    20941 ttggttcttc tcaaattact actgtcttat ttgaaaagaa aaatattgcg tttattactc
    21001 ctaaagaagg aactgacctt gaattttcta tcaaagtaac aaataccaaa gaaaatttat
    21061 tctccattcc tgacattcaa tataccccta acaagttaaa gattggggat acgtttggtt
    21121 tggctaaagt aaatgaacaa tactttgctt actcactgaa ataagtttct aatagcaaga
    21181 ggaaaaaccc tcttgttatt tttatcacat aatcatttta taaaaacgct tcgaaaatat
    21241 caacattttt gaagaaacta tttttctaat ttctcaattt cttccaaggg attttcgcta
    21301 atgacccata caggcacaga ttgctctcga ggctcccctt cttctaacca ccaacctgca
    21361 taatgaactt cttcctcctc agcattacca tgataccaac caacaaagga ttcatctaca
    21421 acgaatgaaa tttcttcttc tggatcaaat aattcatttt tccatgcttc aattgcttgt
    21481 tctttggtgt acttttgttt gttaaataca ccacaaattt cgtcatctac ataaaaaatt
    21541 tcaaaatcaa aattactcgt tccttttact ggcatcttaa cttttcctcc tttgactcac
    21601 ttcaatagat ataccactca tgttcattcc attgtttact agttaattgg agttttatct
    21661 tagggctttg tgcactcttt ccaaaaactt gagcgtcgtt tttttcttct aaccaaaaaa
    21721 ggtaatttct tacattcggt actaaatgtt tttcctgcat ttcttcatga aaaacatata
    21781 attctgcttc atttttcaac caattcatta ttcattccct cattcacttc ttcataccgc
    21841 cattcagata gtgagatttc tttttgtttc tccacttgtc ctataaaaat cgctgtattc
    21901 aacaagctga tcacacttaa taataaagca atgaaactaa tggttacact tactctttca
    21961 cattttgtca ttttgtttcc ttctttactc gtttgttatt tcttgtaata ggtctaataa
    22021 ctccttcacc gaaatttgca aatggttcgc aaaaatttca ataaaagcac tcgcacacat
    22081 taccagacct tcaactgata tatcactgcg acaataaaat tctggagaaa cactgatttc
    22141 tgattctttc ttacaattga acgaaaaaac tatttttgaa tcaaattccg gtaattcttc
    22201 cacaactatg ttttgggatt ctaccaagaa agggagtgcc tgttctaatg cttcttctgg
    22261 tgtttgagca gatagaataa ttgcttcttg ttcttttatt tcattgttct caaatacttt
    22321 ataacttttt ttcattgtta gttcctcttt cattcctctc tcaattagct aagccgttac
    22381 tggtacgcca ttctctaaat ttagtatcgc taatccttaa tgctcgtcga atatcaatca
    22441 ttcgataacc tattgcacga agttctttat atcttttagc agttagcgtc tctaacgtca
    22501 tttcaggcaa tgggttcgaa tcaaaaatag aattcgaacc gattatttgg gccaattgtt
    22561 gtacttgtaa cagctcttct gaacttaatt cagtgattgc tcgattgcct atcattgcat
    22621 acattttctg tcgaatatac ttcttttctt gtttagacaa catttctatc cactcctatt
    22681 ttcattatca tttgttacct ccacttcagt aaatccacaa ataaatgaat tcatattcca
    22741 agtaccattc ccttcaacta caacttttct cctgtcctct ttcggatatt caagaatcag
    22801 accgtttacc aatatgtttt tcactactac taatttgttt gtataccgtg gaattgctgt
    22861 tccgatatac ttcactttat ttcctacgct aattgtcatt tcctcactcc tattcttttt
    22921 tatccaaaac tttttcctgt cctattcctg ccaacgaata tacttcactg catgcttaaa
    22981 caaaacaaaa tgttgccctg ttttatttcc gtcttttact tgttccacat acagttcata
    23041 tttggtcaca ccttttaaaa cgccagatac tttttttcat ccattgtcac aattagtttt
    23101 ttcatcaatc gattgatact gcactgctca tttttcttgt tcatatagta ttcctttctt
    23161 tttcaaaact agcctctttt gtttactagt tttcatcaat catcaacgat ttaacagatt
    23221 ttttctttcg cagggtttct agcgtcagca accccaaact ctaaaaaagt gcctttgctc
    23281 ccttgaaaaa cgagcggacg attgtactcg tcatcatatg tttttcatac tacctgatcc
    23341 acgcaaataa cctttttttg ttctgtgcca cacccatcca tacgtatcgc ttgagattca
    23401 ccatactttt ttaggtggtt tatccttcct aagtccctgt tagacacgct gattaactta
    23461 gaaagtcctg tttgtatcca attttcaaag atctcccaaa agaaataagt aggtttacgt
    23521 gtatcgcttc tgttcaaagt acctgtttgt acttgagtat ttctactccc ctcgttcaag
    23581 ggattccttc caccagctag cttttccttt catcatcaaa tgttcctgaa aagcatttac
    23641 gtttcgtgtc cgtgtgctta cttattcttt tttcgatagc gattatccgc tcgctactcg
    23701 ctattttata ctttgtttgt tagctcaaat aaaaactaac aaacaaaaaa ggctagtttc
    23761 tttccgtttt atttttttaa ctgtcgataa atagttgtta gtttaattcc aagttctaaa
    23821 accaatacgc ccaacaaaac cacaggaaaa acaattgggg caaacaagta aaatcggata
    23881 gttttcatgt cttccctctt tctttttggt gtcgctgtgg tcgttcttgc cgttcaatac
    23941 ttcgatacat tttttattca ttatttgctc gttttgtttt cctctggact gcttttcttg
    24001 ctttttgaat actttatacg cttttcgtgc ctgaattaat aggtcttgtc ggcgtttcat
    24061 tcgtgagaaa acaatatttg aaaccatcaa atcctggttc gaacaaccac gattcgcaat
    24121 aattttccca tcaaaaaaaa tagttttcac ttgagaaaaa aacagttgga aatcttgttt
    24181 tgttgctaat ttttttgtca aaatcactgt gcttttccct gttgaatcta caatgattct
    24241 tcgaacaatc cgattcgata acttccaacg ttgccgatta aacaatgtta cttcacaaca
    24301 aatatgacgt aagtgattag gggtcatttt catgtgtcca attcctccaa ccaattatgc
    24361 atcgaaattt tgggcaattg tgacagccca taaatctgcg tttccaggtc ttcataacta
    24421 caagaacgct tggcttgaag taatgaatat tctgcttgat ctgatagttt tgttgccatt
    24481 ggatctagat tcaattctct tacacaacat tcccaaaaat tcaatagcgc tctaaaataa
    24541 aatccttttc gattctgaat tgcttttcct tcatgacgag caattttttc attttgaata
    24601 caccgttgat acgttttgct taaatcttct gcaaatgttt cccctaaaat tcgatactcg
    24661 cttccatcag ataaaccatg tgtttgatta tactttcgtt gccgcttttc tataattcgt
    24721 ttactgtcgt atatcatgtt tagctgctct ttcgcttcac gccattcagc tgaactgcga
    24781 acaatccatg tcaaaacatc tttcggaaca aacgtacttt cgctgtaatg agcaataaac
    24841 tctttttgcc aatcatcagc catttcaaca ggactagtcg tatcgatttt tgtatctaga
    24901 ttttctgtat ctagattgtt agaagaatta ttaaataatt gtgcagaatt ttcacatgga
    24961 tttttttcgt caaaatcatc gaaaatcgct aaattatcaa cagattcctc ctcttctttt
    25021 ccttgtgcag aatctacact ggttgagtca acaggaccta atgaatacac atcattttct
    25081 gtcgtatcta attctcctag atacaaccga gctttgtttc tttttcctgt ttgttgatca
    25141 aatcccatat cctctatttc tagtagctca tattctttca attctttttt gattgtatta
    25201 acagaacgtc tgcaacaatt caatgcattg gctaattctt gttccgtaaa ttcaaaataa
    25261 acaaaacctt gactatctac ttgatttcgt tgaatggcta aatctaagcg tgaccgaaaa
    25321 accatatacg cgatttttgc tttatcagac aatgagcgat atttttcact tgtaataaat
    25381 acctttggaa attgatagta taattcattg tatttacgag ttgcggtaaa tctttttgtt
    25441 gccatcgtta ttacacctca tcttgtccat atattaagaa agaatattgg tgttggtaga
    25501 cacgaacatc ttccattgta gcaatcaatt cttttagata aattcgaccg aattcaattg
    25561 ttagtaagtc gtaatcttct aattcatttt tgatttcttc aatttcttct tcagacacat
    25621 tgagtaaaat ggctaattct tgattggtat aagaaatata gacatgtttc ttttcatcaa
    25681 tccgtttttc aattaaggat tcttctaagt actcattcag taagctatag gcgtattttg
    25741 cttgtgcagt catcgcataa tattttgacg attttataaa gaatagtttt ggcatatgaa
    25801 aagttgcttg tgtcagcaca tctgttgcgc gaaatttgtt ttttaccata ttttttcctc
    25861 ctagaaaaac acgcaaaaaa agctctgtat aaatcgaatt acacagagtt tcttcttgtt
    25921 gatatttaat ttctaaaata cgtttcgggt aaaaactaaa cacttactaa tatttagatt
    25981 gtagaatctt cgccttcatt tattcatttt aaagagaaaa agatcaagtg aaatgtgccc
    26041 aaaagtttga ttctattaat ttttaaacac cattcataat atatttccta taatgtaaaa
    26101 agcgtcgata cttattaaag catcgacgtt atttttatag taaatttatc aaaatttttg
    26161 ccaacatttt ttccgatttt tttccctatt tctatctttc cttatctttc ttagttaata
    26221 ttatttgatg ttaatatatc actactaata ttaaaaaaca ttgatttatc ataggataaa
    26281 gagtgatagt ttaatactta aagagacata ttaattttat ttaaaaattg acattaatga
    26341 ctcttaatca gcgggtcgcg ggttcgagcc cctcacggcc cattgggtgc caaacccacg
    26401 gaacaggtaa atgttggtgc gaaagcattt cgaaaacatt tacgctgttc tttttttatt
    26461 t
//
LOCUS       DACEDM010000043        24909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEDM010000043 DACEDM010000000
VERSION     DACEDM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.043"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(94..1407)
                     /locus_tag="IWO50_001616"
     CDS             complement(94..1407)
                     /locus_tag="IWO50_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0358740.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(1652..1876)
                     /locus_tag="IWO50_001617"
     CDS             complement(1652..1876)
                     /locus_tag="IWO50_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358741.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(1896..2264)
                     /locus_tag="IWO50_001618"
     CDS             complement(1896..2264)
                     /locus_tag="IWO50_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0358742.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(2461..3171)
                     /locus_tag="IWO50_001619"
     CDS             complement(2461..3171)
                     /locus_tag="IWO50_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358743.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(3189..3686)
                     /locus_tag="IWO50_001620"
     CDS             complement(3189..3686)
                     /locus_tag="IWO50_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0358744.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(3723..4175)
                     /locus_tag="IWO50_001621"
     CDS             complement(3723..4175)
                     /locus_tag="IWO50_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0358745.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(4310..4915)
                     /locus_tag="IWO50_001622"
     CDS             complement(4310..4915)
                     /locus_tag="IWO50_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0358746.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(5365..5985)
                     /locus_tag="IWO50_001623"
     CDS             complement(5365..5985)
                     /locus_tag="IWO50_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0358747.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(5987..6856)
                     /locus_tag="IWO50_001624"
     CDS             complement(5987..6856)
                     /locus_tag="IWO50_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0358748.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(6879..7286)
                     /locus_tag="IWO50_001625"
     CDS             complement(6879..7286)
                     /locus_tag="IWO50_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0358749.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(7305..8594)
                     /locus_tag="IWO50_001626"
     CDS             complement(7305..8594)
                     /locus_tag="IWO50_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0358750.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(8621..8923)
                     /locus_tag="IWO50_001627"
     CDS             complement(8621..8923)
                     /locus_tag="IWO50_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0358751.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(8948..11041)
                     /locus_tag="IWO50_001628"
     CDS             complement(8948..11041)
                     /locus_tag="IWO50_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0358752.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(11530..12294)
                     /locus_tag="IWO50_001629"
     CDS             complement(11530..12294)
                     /locus_tag="IWO50_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0358753.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(12405..13274)
                     /gene="mdcB"
                     /locus_tag="IWO50_001630"
     CDS             complement(12405..13274)
                     /gene="mdcB"
                     /locus_tag="IWO50_001630"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0358754.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(13299..14375)
                     /locus_tag="IWO50_001631"
     CDS             complement(13299..14375)
                     /locus_tag="IWO50_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0358755.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(14399..14992)
                     /locus_tag="IWO50_001632"
     CDS             complement(14399..14992)
                     /locus_tag="IWO50_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0358756.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(14996..16672)
                     /gene="mdcD"
                     /locus_tag="IWO50_001633"
     CDS             complement(14996..16672)
                     /gene="mdcD"
                     /locus_tag="IWO50_001633"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0358757.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(16662..16961)
                     /locus_tag="IWO50_001634"
     CDS             complement(16662..16961)
                     /locus_tag="IWO50_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0358758.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(17063..18709)
                     /gene="mdcA"
                     /locus_tag="IWO50_001635"
     CDS             complement(17063..18709)
                     /gene="mdcA"
                     /locus_tag="IWO50_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0358759.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(18716..19627)
                     /locus_tag="IWO50_001636"
     CDS             complement(18716..19627)
                     /locus_tag="IWO50_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0358760.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            19747..20604
                     /locus_tag="IWO50_001637"
     CDS             19747..20604
                     /locus_tag="IWO50_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0358761.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(20791..21153)
                     /locus_tag="IWO50_001638"
     CDS             complement(20791..21153)
                     /locus_tag="IWO50_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358762.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            21499..22938
                     /locus_tag="IWO50_001639"
     CDS             21499..22938
                     /locus_tag="IWO50_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0358763.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(22945..>24909)
                     /locus_tag="IWO50_001640"
     CDS             complement(22945..>24909)
                     /locus_tag="IWO50_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0358764.1"
                     /translation="VQSGSAVGVVVETGNTTEIGKINQALQSVEQQTTPLIKKINRLN
                     KQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIALVVSMVPKGLPAVLTMILSVGVH
                     EMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNEMTVVDVAAKEEACVLSIMKNCQ
                     ELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKIEAKIPFSSEYKYMATMHAEEKG
                     AVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARKGQRVLGFAYKKVDSKQELTHET
                     LTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKMITGDHKDTAKAIAEQIGLKHTA
                     KVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLRIVKALQKNGEIVGMTGDGVNDA
                     PALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTIAKAVKEGRRILDNLQKTINFFL
                     PTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTITLSYELGFESPDPEIMKRPPRD
                     PKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQSLQQTILLQNIVLAQAVYMINCR
                     ELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPVGQQLIGTVSLTMTQQLLIGANV
                     ILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
BASE COUNT         7080 a         5293 c         4266 g         8270 t
ORIGIN      
        1 tcggtattag ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa
       61 tactcaggcc agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa
      121 tcccacaaat acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc
      181 tgctaacatg gtaattggat agaagataaa acctaatgca atcccatctg aaatactata
      241 agtcaaaggc atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca
      301 ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt
      361 tacttgtgaa gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat
      421 ccctgtagta atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc
      481 tacaaatgca ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc
      541 agaagctaaa gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt
      601 tgctaatcca actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac
      661 tacgacccac atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt
      721 tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac
      781 cagacctaaa atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac
      841 aagcaagata gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag
      901 tgatcctaat cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc
      961 aaggaaagcg ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat
     1021 cgcatcaatg atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc
     1081 aacgaatacg ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata
     1141 agcaaaaaaa gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac
     1201 ccccattaaa acacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc
     1261 atccattcca gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat
     1321 aaatgttgta aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc
     1381 gaagtaggaa agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata
     1441 aagtccgtta aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa
     1501 aacataagta aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa
     1561 tccctttatc tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt
     1621 taataaagtt tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc
     1681 gtttctgttc ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc
     1741 caaaacctgt atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat
     1801 tctttgctcg aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc
     1861 ttcccattag atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct
     1921 ataacaaaat aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct
     1981 gtgatttctc ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat
     2041 actaaagaag gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg
     2101 aatacgccta attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc
     2161 aattcgattg tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc
     2221 tctacgtcat tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt
     2281 ctatttgatt ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac
     2341 cttgtgtttg atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca
     2401 caaacttttt ttacaaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt
     2461 ttataattct gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa
     2521 tgtttgatcg attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc
     2581 agcattgatg gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcattgttct
     2641 ttgggcttca cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat
     2701 ttcttccgga agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc
     2761 ttgggctttt cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg
     2821 tacgccaaaa gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa
     2881 ttcccgttct tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt
     2941 agtcttgaat gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt
     3001 ttcttctgta aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt
     3061 ttcttcactt tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt
     3121 tgcttcagga aatacttccc agaaattggt atccactatt acttttttca tctgttcaca
     3181 cctcttgatt agaataattg cttgatgaat agatagtctg tttggggatc atctcccata
     3241 taaaaaacat gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca
     3301 tcgttgtgtt cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca
     3361 atagctgtat ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag
     3421 attcgctcga tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt
     3481 agtttcaggt aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta
     3541 tcttttagct cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa
     3601 tgctccggtg aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt
     3661 agtaagtcta catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc
     3721 tttcaataga tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg
     3781 catactttat ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat
     3841 gcgactgcat tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt
     3901 ttagtcggat agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc
     3961 ccattggatt ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt
     4021 tctgctagtt tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac
     4081 tgtcctcttg ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg
     4141 gcaatcattc cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt
     4201 aagactagtt tacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc
     4261 ttttttaata actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat
     4321 agatctggtt tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata
     4381 gctttggcaa ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa
     4441 acttggcctc ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct
     4501 gtggaatatc tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg
     4561 ttaatcctgt ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt
     4621 cagcaaatcc accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct
     4681 tgccatcttt tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa
     4741 tcgttgtgag cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca
     4801 atgaaatcgt ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata
     4861 gaccagcttg agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct
     4921 tcccttcttt tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta
     4981 cggcaaataa atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca
     5041 aaagacattc gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg
     5101 taataagccc caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa
     5161 ttgaggcagg acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct
     5221 cggtcacaag tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg
     5281 tgactcggag ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt
     5341 tttcgatatt aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc
     5401 caatagtgga gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc
     5461 taactggaat ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat
     5521 atgattggtc cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat
     5581 atagctcaaa tgacgatctg attcacgatc acgtttgtac cgacgttgac ataacacttc
     5641 aaagtctgct gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg
     5701 gagtcgtgct ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc
     5761 ttccatatac aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc
     5821 cgcccgttct tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc
     5881 gagcgtgatg gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt
     5941 tcctgtacca ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta
     6001 agttaattgg actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta
     6061 ctgcttcatt tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa
     6121 aggtttcttt agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc
     6181 ggatcccgtt attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga
     6241 taactagggg gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg
     6301 tttttgattt gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg
     6361 tagcttctac aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga
     6421 catggattcc ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt
     6481 gcgcatgggc atattctact actgcttttg tcttcaggat attttctttg aaaggcagat
     6541 gggacccatc atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa
     6601 agtctcgggc gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg
     6661 tcattacatt gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa
     6721 taatgatcgg cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt
     6781 tatgcgtatt aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt
     6841 tcaatgttgt atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt
     6901 acttgttttg caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga
     6961 tcatcaccag cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta
     7021 tacccatctc cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga
     7081 tcgttcacat acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta
     7141 tgcttatctt gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt
     7201 tcggtcgctt ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt
     7261 tcttttttct taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa
     7321 actgggctta agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct
     7381 gtagcattga tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta
     7441 ataaagaaac catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg
     7501 ccaaatatcc ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc
     7561 gctaagccga acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg
     7621 aaacctaaaa tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg
     7681 atcgcatcag gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac
     7741 agtagacgta caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa
     7801 aaggcaaaca tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca
     7861 aataaaacag tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct
     7921 tgcagaaatt cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct
     7981 ttctcaccaa aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga
     8041 cctaaagcaa tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc
     8101 atagcaatgg cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc
     8161 agaccggcaa aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca
     8221 gttaaaaaaa tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg
     8281 gctaagaacg tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg
     8341 atagattcaa tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg
     8401 aaataagtta aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg
     8461 agtgtcttta acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc
     8521 aacatagcaa tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa
     8581 ttgataacgc ccatgttttt accctctttt ctttttgtat ttagataact cccttatctt
     8641 gcagaactgg ggtgattttt tcttcaattt cattttttga aacaatattt ttcaaataga
     8701 tcatcgccgt tttaggatca actgcgaact tttcgaactg cgaacgaaaa ttttctgctg
     8761 tgatgatcac atctggtttc atggaaactg cggaagaaat atcacaatgg tccagtttgg
     8821 cttctactcc taatttgttc aaaacatcct cagcagacat ctgtgctgca aaactacttc
     8881 ctaaacctgc accgcatacg aataaaatgt tcaatttgtt catctttgtt ctcccccgta
     8941 tcatttatta tttctcaact actgaaaaaa gcagttgttt gaaatcttcg atatttgtgc
     9001 attcaatcaa tcgatccaac ttctcttcgt tgttgatcaa ttcaattagt tctttcatga
     9061 tgttcagatg cgagtaggag tctactgctg ccaaacaaaa aataatcttt actggatcca
     9121 tttcaggatt accgaaatcg attggttgct caatcgtagc tacgctgact cctaatttat
     9181 tgaccccatc ttctggtcgg gcatgggcta gtgccatgtg tttgccaatc acgatatacg
     9241 gtccgtaatc attcactgca cgaatcatcg cttcgacata gcgctcttcg acaatgtttt
     9301 ctttaaccaa tggtctagca gctagttcaa tcgattcctc ccaagtagtg gctttttctt
     9361 tgatcatgat attgctgtcc gtcaaaatat cttttaacat aggctggatc tcccttttgt
     9421 tgatttctaa attgtttgtt tcgaataatt tttctaactt gccatagatg attcgatcta
     9481 ctcggccacc actttctttc atgacatcta ttatttgagt gaataactcc gtcgattcat
     9541 gactgttagg ctctaaccgc tgtagttctt catttctttt caaaaagtct tctataacca
     9601 agatattcgt ctcagttaaa atcggatcga caactaaaag cggcttattt cgataattca
     9661 attgaaaggt gctgaaaacc agattgacat ccaatttatc tactaattcg atttcttttg
     9721 aacttaaaac tgcagtaatc tcgatctgag gaaacttttc tttcagattc tctgctaaca
     9781 gatttccagt agccatccca tggttacaga caacgactga tttataaaca taattcaagt
     9841 ctcgcttgat tacactggcg atcgtcgaaa agtggatgac aagaaaagct atctcgtcct
     9901 ccagtatctt gccaccaatt acttcttcga tcgtaggaac aaaatgagtg actgcgcggt
     9961 agattgggaa ataatttgtt cggatgtttt ctttcaatgg attcatgact tggatatgat
    10021 tcttgattct tacaatcagt gcagccatgt gcttatagag attttcgaat aaagcttctt
    10081 cctttagatg gaaaggtacg tgtgtctcgt tctctacaaa ttggatcaat tggatcgtta
    10141 gtaattgagc ttgtacccac tcgattgaat tggagatatc tctagcattg aacgtctcaa
    10201 tagtgaaacg aagataattt ctttctactt gcgggacatt gtccaattga aattcctcga
    10261 tcatgtggtc aataaatgca gaaaagctac tgtcttttat ttctgtattt ttccaattaa
    10321 tggcagagat aaattcatgt ttcctgattc gctggatcca gatcaatgta aaaagcagga
    10381 tttgtttttt ataaatatca tcttcttgtt tcaacaactt attggaatag atctcctgta
    10441 tttttttgat ttccaccaaa ggaaaataat ggagaaagat tttttgagcc ggtgttaatt
    10501 gtgcatcttg attcataaag aagtcggtca ttcccagatt ctgattgata aaatcataga
    10561 tcatcgtacg gatagatcgt tctgaacctt tataaaccag tccttgcttt ccataactga
    10621 cgatctctat cccataactg accagtgttg ctcgtaaccg tctcatatct tcatctaatg
    10681 tacttttaga gatttgaaat tcttcttctt ttttgtttag atggatcggg attcggctgt
    10741 aagcaacggc taaaactaga tcaaaaatac gttcgcgccg attcaaaaag ccgtctttca
    10801 catcttttat ttcagtttct aatcgagctt gcttttcctt ttccagcttc aacaaaaaaa
    10861 ctttgttgcg aattgtacgg atctccggaa gctttttact gctcaaccag atattcgctt
    10921 caagaacttc ctgacggatc gtccgctgac tgacattgaa ctcacttgct agatgttttg
    10981 cttttactgg atattgggct gtcatgattc tgtgaagtat ttcaattgtt cttgttttca
    11041 tagaaatgat ctccttctga tttcaagtat atggttccgc ttacataata aaaagtagat
    11101 atttctgcca cttgaagtgg aaagaaagta agcaaagcaa acaaaaaata agccatatgc
    11161 tttttcatct cctcaacgaa aaagacatac gcctattatt acaaaaattt tgcccatcta
    11221 tacaaaaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca actaaactaa
    11281 aatagtgctt cctatttttc gtttggttgt tcttattatc gcagatgcaa gcttttaaac
    11341 accatttagt ggagaggttg tgacagagat gttcagcctt gagcgataag aagcgatttt
    11401 gaaaaatcgc ttcccatatt ttacaaaatc gtgccttatt gccgataggc tatctctggc
    11461 acactgttta ttcagaaaaa ggagctgcga caaggctttt gtcacaactc cgttcataaa
    11521 tatttcctgt caatccagca taatcggctg aataaaatcg gcttgccatc ttttttcttt
    11581 ctcagaaaaa tcaaaatacc atatctgaga aaaatactgt cgttgtgtct tacttcctga
    11641 gatcaatcgc ccatcttttc gatcgactga ataatcacag catctgaaat caattcggat
    11701 ggcaaaacta ttttcgtgga tctgtcgata atttcctact tcctgaatag ttattttttt
    11761 agtatgattt cttacaccgt ctgctttgtt tttttgcaga actttctgat gttcggcaaa
    11821 cagcttgtct gtataacagc ttctggcttt ttcaggagtt tcttcttccc aagcggattg
    11881 gatcttcatg aatatctttt ggatttccag aaactgtgct ttcttttctt tagccgttcc
    11941 tgacatgcgg gacaatatct ccgctttcag ccttttcttt tgtcttttat tcttgacgat
    12001 agaaaaagca gaaacccctc ctactgacaa aacagctaat gcaagaaatc ccgtttgctc
    12061 ataactagaa ggacctgtac ctggaccata atacccataa gaatctctgc cgtaagaacg
    12121 actgccaaaa taactgcccc catatgaacg accaccaaat gaacctctgc ccgaacttcg
    12181 accgctagat gtacctccac catgaccacc cgcgacagcg aatccttggg tcggcagagc
    12241 aaacaaacat aggcccacta acaaaatgat tgctaaataa ttgatttttt tcattgtctt
    12301 cctcgttatc ttttattaca aaaaactatt tttatgatag ccttgctaaa aggaagagtc
    12361 aataaccaga tgcataaact gtttttttag caattttgtc ttatttagtc attgagtaac
    12421 atcggacaaa tgtatgtgtc ggcagtccat aattcctcta tcttcagcat gaacaagctt
    12481 gcagacaaga gatctgcgct tcctccagga gagatttggt tttcctgaca aaagtgcaac
    12541 agtgctgtaa atggacgatt tggtaaagca ctttgactag cagcatgagc aagtttttgc
    12601 atttccacta acgttttctg atcactgcga tgcaatacgc atgtgtcatc tagcgaactc
    12661 atcaaaacta acaacatatg catttgtttc tgtacatatg aatcattatc tagctcctta
    12721 gctgcttgga ttgcaagcct gatatgcgga taccctaata tcgcttcttc atatgcacca
    12781 tttacgccat atttttgctt caccctatga ccatgagtgg tttttttatg acgatatttt
    12841 gtatcaggaa aagcagcaag cttgctgaca tcatcaagta ttttcgataa agaaaaaggc
    12901 tgttccatac tgattttttg tgcaatgaca ctgatcaata gccccattgc ccaaatcgct
    12961 cctttatggg tattgatgcc atttgtagca atcagcattg ccttttccgc ttttcgccca
    13021 atctcagcaa ttctttctct taactcctga tcaatagcat gatgccatgc tatttgagcc
    13081 atttcttcaa aaaaaggagt tagcgtctta gcagacttaa gaaataaagc atagtccata
    13141 tcggaatgtg cacccgcatc cagtgcatcc actaatccag gttttggact taatgttact
    13201 tctgcgatca atgcttcctc taccaacgca gcgagttgct gagtcactgc tttttttcgt
    13261 tcgtcattta tcatgacact cactccttct tcctactatt acttgctgaa accttttttg
    13321 atagctaccc aaatgatatg aagtaaccag cttaaagcaa atgcacaact tgccgtcatt
    13381 agataagcta caagaatcat gactcctgaa ggtaactgca gccaactatc tttaaacaat
    13441 aataacagta cttggaaaac aaatacattg gctaaatacg tacgataagc ataaacagac
    13501 aaccatttga acaatggcaa ttttggcgaa tcgtttacaa tcattttgga agcgcctatg
    13561 aagacagcaa agatgatgac taaactgtat aaactttgga tcgtattcaa gtaaggtgca
    13621 ttcgcaaagc tcaagtctcc tggatgtgcg agcaatgaac ggacagaaaa gaacgcgata
    13681 actaaggcaa caggtaagaa aagataacgt actctgtcca aatattgata gactttttcg
    13741 tgataaacaa atgcggctac cccataaatc atgaaactag caacaaaccg atctaataag
    13801 taccagctgt catgataagg cccagtcaat acttgtgttt gatagaacag ataccatgcg
    13861 acatataaaa tcgttgaacc aatcaatact ggccatacat attttttatt agcaaatact
    13921 ttgtaaccga gccaaacaaa aaacggcatg aacaattgga tctgcagcat cataacggta
    13981 taccataaat gaggcgctcc atcacctgtg acatatttca gtaaaaagct tgcagtatcc
    14041 gtatacggag tttgttgttg gactgctgga aatagccaaa gataagccgt cgtccataga
    14101 agatacggaa cgactagttc aaaaaacttt tctttcaaaa attcccaata agacaaatgc
    14161 tgacttgttt ttaccatatt ataaacaatt gcaaaaataa acattggtgc tgtgtattta
    14221 acgatgacgt aaaaggtacc aagaaaagct gtatttgttg cgctgtggct gtttgccaaa
    14281 gcaaagctca atatcgtttg catcataaca gccgttacag ctgatacttt ctggaagtca
    14341 cttacgttga ttttgatttt tttgttttca aacatctctt tctctctctt tttctttatt
    14401 accaaagttc tttggttaat ttaggtcctt cgactgtttt caatagaatt tttttctctg
    14461 gctctctcaa atactctttt agtgaaaaac cgccttgccg agtcactact tgggtatcga
    14521 attgctcaaa aaaagcagga tgcgattgaa ttacctgaat tggcaattca atcggtgaat
    14581 ttgcgtacag cagcaaatca aaatcactgg tttcttttac tgccggaatc ccagtcgata
    14641 attcaaatcc taagctgccg ccgattcccc atttacatcc tgataatatg tttctagcag
    14701 catgaaattg ctgataaaca ggataatcca tgattttctc ttgtctatac gattcttgtt
    14761 cgatgatgtc ccacggatga atgacttgtt tgacggcatg ttttgagatt tctgttgacc
    14821 atctctgatt gcgggcaggt ccacgtaaac caatcggtag tatttctgcg tgatccgtcg
    14881 acgcacgacg aactgttccg aacatctttt cagttagaac gacccattca ggcaaggtga
    14941 acccttctat ttcatgttta tcaaaaagga caatatcatg tgcttgaatc attttttatg
    15001 catcccattc ttcgtccatt ttggcacgga ctttatttgt ttcgacacgt ccgaatgttt
    15061 tggctgtttc gtttgtgtat cgatttgtca gcatcgtgtc atttgtattg cggacatctt
    15121 gaatggcttc attgattgct tcgatgactt tgctgacatt ttgttctgaa gcagcttggt
    15181 cggtgatatc ttctaggaaa tgatagatcg ctcctagttt tgtataatta ttgatatcgt
    15241 aagcgatagc tggaactttt tcagcagctg cttcgatttc agcaaccgaa cgttttgtca
    15301 cacgtgcagc actttcttta gacatcgctt ggacggtaat ttctttattg tttaaagcaa
    15361 tcaagcggct tgattgtaat ccatgtgcta aaaatgcacc tgaaatagca tttcctgcaa
    15421 tcaatccaat aactggatgt ccagcttgac ggagttttgc ataagcagca gcactattag
    15481 ccaaagccac atggataccg atcaattctt cgttatagcc gtatgcttgg cttggtacat
    15541 cgatcacaac gatgattggc cgtttcactt ttttgtcttt atcttcttcg taaacgtgat
    15601 ccaaaatttg tcccatgcgg aaaccttcaa ctaaaccaac ttcaccgttt cgtacacggt
    15661 acatcgggtt tttaggatca ggtacgattg atgtcacgat ggcttcttct atgaatttac
    15721 ttgatttacc ataatacaca gtactaatag aagaagttgg atttttcatg ccagttagtg
    15781 ccgcaaacca atcgtaacca acacttgctg cagctggttc atttccttct gctacttctg
    15841 gtaagtcaat tgattttgct tgatggttag caattgcttc attgtaatcg tcaatggcca
    15901 ttggttttga aagatctaaa gaatcaagta atgataaata aaatgctgag ctttcactgc
    15961 gatgcgtgtc ttttcgttcg tccattgctg cgatgacagc agctttgatt gcttctacac
    16021 tatcagtcac taattcatca atgatttttg attctacccg ttgacgagat cctaatgtat
    16081 tccaaatcaa gtctttgtca ctagaatcaa attctcgaac gcctgcttct tgttcaataa
    16141 cttctggtcc attcaaaccg actctcgctt tttttgttgc gattaaataa ctcattaatg
    16201 ctgaagtaat cgacataccg ccaaatgatc ctacacgtcc tggaatcaaa ccgatgactg
    16261 gtacatattg ttttagagca atgattacat ttccgatttc tgaaatagat aacagtccgt
    16321 agtttgcttc ttgcaaacga acaccacctg tatctagtac aatgactgga aagatctcat
    16381 ttcctttttt attttcttct aacacatgtt caagtgcagc aacgattttt gcaccggaaa
    16441 cttcaccgat accgccacct tggaattttc cttccatagc gattactact gcttgtttgc
    16501 cattgatcgt gccacggctg acaacgatcc cgtcatcact ttcagggacg attccttgtg
    16561 ctggtaaact tggcgcgatg atttgatcga atggatcaat caattcacgt ccattgtcaa
    16621 gtagagcgtt cactcgttct ctggcgttta attctacaaa actatttttc atctgaaagt
    16681 tcctccattg cttgcgtcaa tcgtaggtac acaactcctg gtgtagcacc gaagtcattg
    16741 atcacgatat ctgctgaaat tggataacga tcaaaaaatc tcgttaatac attgttccat
    16801 acttcgccga agccatcgct tcctgtacaa atttttacag tagattctgt tttttctgtt
    16861 ggaaacatca agatttccaa atctcctgaa ccgaccactc ctacatggat tttcttattg
    16921 atcggctgtt tcgttggata tttataagct aattgttcca tggatctctc ccctttttgt
    16981 aaaacagagc atacacgttc tgcttattcg tctttcttct tccatctggg taaatggcga
    17041 caaaaggttt ttctcgccaa ttttatgacc aagttctgaa tttcattggt ggttcataca
    17101 ggccatctga ccattcgatc aaatcatcga tggtttgtgc agctaataat gaacgtttcg
    17161 cttcactacg tttgatgcct agatcttctg gtaatttcac gatgccggct gcacgcaagc
    17221 tatctagtga ttctttcgtg ctagtcattc caagaggtgt aacaccgcca attgctgcaa
    17281 ttgctgcttg tcgttcttct tttgtttttg ctttgtacaa ataagcaatt ccttcttctg
    17341 tcacgatatg tgtagtatct tcgctataaa tcatcactgg tgcattggct aagcctgctt
    17401 gtttttgaac agctagtgca tctaattctt cgacaaagac tggtttttta tttgaaccat
    17461 atgtttcaac catttgaaca actaattttt ggcctttccc taatggatca tcattgtcgc
    17521 gaagtgattg ccatgctggt gtagaatgac gacgaccgcc aggattatgt cccatgttag
    17581 gcgcaccgcc aaaaccagat aaacgtcctt ttgttacggt agaagagttt ccttcgtaat
    17641 ctaattgcaa ggttgaaccg ataaacatat cgatggcata ttgtcctgca gcttgtgata
    17701 atgttcggtt actgcgcatc gtgccgtctt taccgacaaa aaagacatct gggcgagcag
    17761 caatatattt ttccatacca acttcgccac caaaactatg gatgctttca acccaacctg
    17821 attcgatggc tggaatcatc gttggatgtg ggttcaatac ccagttcttc gcgattttcc
    17881 cttttaaccc tagtgattca ccataagttg gcagtaacaa ttcgatagct gctgtattga
    17941 atccaatacc gtgattcaaa gattgtacgc cgtgttctgc gtagattcct ttgattgcca
    18001 tcattgccat tagaatttgc aattctgtga tgttttgagg atctcgtgtg aataaagctt
    18061 ctaattgata aggtttatct gctttcacga caacgtctac ccagtcacca gggatatcta
    18121 cacgtggtaa agaatcgaca acttcgttga cttgaacgat cacgatacca tctttgaaag
    18181 ctgcagcttc gatgattgtt ggtgtttctt ccgtattgaa acctgtgtac aagttgccgt
    18241 ctttatctgc tttatctgca gctactaaaa caacatttgg aatcaaatcg atgaataagc
    18301 gtccataaag ttccaaataa gtatggatat cacctaattt cattgtgccg tcttcaatca
    18361 tttgggcgac acgcaagctt tgtgcaccag cataagagaa atcgatcttg cttgcgattc
    18421 ctttttcaaa aatattcaaa tgttctggtc gtgagatact agacatgatc aaatgcagat
    18481 cattcacttt ttctgcatct actttttcaa gactttctga aagaaaactt gcttgttttt
    18541 gattgtcgcc ttctaataca actttgtcgc cgggagcaat cagtttttcc attacttcta
    18601 cgattttttc agtttctaca tacttgccat tgagcaagtt gctgattttt tccaatcgct
    18661 gcgctttttt ttcgcgttta gaagcccagt tgcggtttgg tttttccata attagtcatc
    18721 tccatttttg atatagaaac gtggcgtcat caataccatg ttggtctaga ttgatcgtac
    18781 ggatcccttt atctacacca aattttgtat gaatcagctt tgtcaaagta ttgccggggg
    18841 gaaattcaat cgtcagatcc attcctaatt cttttgccaa gtccatcatt tggttccact
    18901 gcacagggaa agctaggttt tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg
    18961 ctcttccatc cgtatttttt atatacaaac atctagcatc agatagatgg acagactgca
    19021 tatacggctt gaatttcttg accgtatcat tcatcaagag acagtgagat ggcacaggga
    19081 ctttcagcag cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac gtaacggata
    19141 aaccttcgat actcccagat aacgtatgtt gcatcggaca attttcattt gacagataaa
    19201 ctggatggtc aggatcaaat gtttttgcta caatttcctc agcttcgctt ctcgtcacac
    19261 ctactactac gcccattgca taaccgcttg gataagcttc tttcattaag actgcgcggt
    19321 gatagactag ttccgctgca ttctcaaatc tcaaagatcg acaagcaaca gctgcacaaa
    19381 aagcaccaat cgaatgtcct gctgcaatat caggctgaat accgtttttt tccatttcat
    19441 ctaaataaaa tactgctttt gtcagtaatg ctagctggat aaaaaccgta tcttgatagt
    19501 tttcttcttt atcaactaac cgataccccg tcacattttc taaacgttcc atgtacattt
    19561 cagggatatc cgctaacatc cctaacttct gactgccttg tccaggaaat aagaaacctg
    19621 tactcatgag cctcgcctcc tcagcactta ctttttccac tgctatctta gtcaatttct
    19681 gctataataa aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg
    19741 ataattatga aattgcttca tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt
    19801 acccaagcag ccaaagaatt atttatttct caacctgcct tgagccgtgc gatcaaacat
    19861 ttggaaaacg aattaggtgt ccctttgttt taccatagcg gcagaaacat tgagacgacc
    19921 gtttatgcag aagaatttta tccatatgca gtcaagacat tagacacatt agaagaagga
    19981 atcaaattat tgtcttcggt caatgagaag atcgttactt ccgtcatatt gtatttagag
    20041 gtagcctctg tttctattcc gaacttggtc cgaattttta tggaaaagca tcctgatatc
    20101 caattaacga tcatgcaaca tcatttacca gatgatatta ccgatcctgt actgtacatc
    20161 acttcggaga tgaagcctgg catgaccaat attcctatca tgaaagaacc tgtttatgtc
    20221 gccattccta agaaacatcc tcttgctaaa aaagaacatc tgcaattaga agatatccaa
    20281 cgcaccccca tcttgatgct gtcaaagagc aacgccttcc gtcatacact ggatgcagca
    20341 cttgaagcga aaagtatcga attgacgatc agttcgacga cagatgatcc tgcgacatta
    20401 cgttctattg tcaagcaagg attagctgtt agctttttcc caaaagtttc ttggtcttac
    20461 gataaaaatg atcctttcgt cttgcgggaa atcaaagatt tgcccttgac acgaacaatt
    20521 tatttatctt ctagcttcac agaagatcat cctttgatca aaatgattgc aaaaacgatg
    20581 cgggaatttt atttaggaaa ataaaaaaaa aagagctggg acaaaattct tgtcgcagct
    20641 cttttttcga acaaacggtg ttccagaagt aatttcttag aaaataagcc gaaatttccc
    20701 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagcta aacatttctg
    20761 tcacaacctc ttctctattg tcctagacat tcattgcgtt gatttaaaac ttccatcaat
    20821 caaatctgct agttcatcta gcttttctat tttacgtaga tcaatagaga tccaatgttc
    20881 tttattcatg tgataggctg gaaaataccc ctcttttcct cgcaaaattc cgccaagttc
    20941 aggatcgacc ttcaaatcaa gaatattgat gatcctatgg tcacttgcat catcatagat
    21001 tttttcttct ggaacagaca tcaggacccc aaaccatttt ttgctttttt gatgtctgaa
    21061 tacgcaataa tcaggaaatt ttttaaaaat atattctgga gacaaattgt atgtttcatt
    21121 gatgtgatcg attagttctt ttttcgttag catctgcttc catctctttc ttattgattc
    21181 gctttattca aagataccac gtaagaatag aaaaatgaat acaagtagtt gctaatcaaa
    21241 tacttcgata tttttctttt ttatcaactc ttaaatgttc aatccgcctg tgttggtctt
    21301 gctaccatct actattaaaa tgtttattat tgtttgccca cctatttttt gattttagtg
    21361 gtatcatttt tcaagttcat gatgaaattc ttatcatcag taaatgctcc agaaaaataa
    21421 acaatgattg aaaaatcagt tgaaacttga ccaccgttta ttttctccgt attcttagaa
    21481 tagaagggag tctgattgat gtattcacta atgaaaaaaa tcattaccga aaaagatatt
    21541 cgacgtcatg tttctttagt agaacaattg ctgaatcata caaagataac cgtaaatgaa
    21601 ttagctgaaa tcatagggac aaccgaacga acgatttttt ctgaccttca atctattcgc
    21661 tctcagctgc ccgaaggctg ggacatcatc agcgatcaag caggaattag tttgcaaaac
    21721 cagcaaaatt tattaacaaa tgacctttgg gagatttttt tcaaacaatc agtcagtgtc
    21781 gaactgctga aaaatctgct ttttacaaaa aaagttgctg tccctgattt tctagccgac
    21841 catgggcttt cttatgggac attgaaaaga catgtgacta aaatcaatca gcgtttagca
    21901 agttacgact tgcagatcga tttgacgaaa tatacagctt gtattcttgg aaaagagcgt
    21961 gcgattcgta ctttttatca tcgattgctg attcctttca ctcataacaa ctattttttc
    22021 gaggattatt cgatccatga atcccattat tttcaatttt taagaaacct cagtcaaact
    22081 gaactagcgg ttgaaacaga agagattttc ggaacatgtt ggttctttat caatacgatc
    22141 cgtataaaag cgaattgccg attagattct tctatccata ccaacagcac actctcctcc
    22201 ctgtatgact ctgcattgaa aaaattgtat ttaacggaag gtatttatct taaagacact
    22261 gagctttctt ttgcttcttt ctgctttctg gaaagttgga attacaacaa taactatgga
    22321 caagaaaccg ctcgctgcct gcatcattct ccatttttag aggtcctcga aacgtttgta
    22381 gaggaactag cttcagagct gtctcttgat caactaaaaa aaacatctct gactgataac
    22441 ttggctctcc tcatattaaa atatcatgaa tcgccgatat tgattgaagc attggaccgt
    22501 caataccatc gttttttaga aacatatgat cagcaatatg cacacttgta tcaacagaaa
    22561 gagaatttac tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa ttactttctc
    22621 caactcttgt ctctactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat
    22681 gtctatttta ttttccaagg agaaccttca tggaaggcgt tcttgcagca ggaattaaaa
    22741 gattacttag gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt
    22801 tcttttgaaa aaacagatat cttgatttcc aatttccctc tagattctct agatatccct
    22861 attgtataca tttcttctat tccaacgaaa aatgagctca atcgtttgac tgaactcact
    22921 ttcaaaactt ttttatagta acggctagcg actgtattct ctagctgttt tttctttttt
    22981 gcgaaacagt ttgttcatga tccctttttc tatttctaca actaagaata gaagaatcac
    23041 atttgctcca atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg
    23101 gccgactggt agaaaaataa ccatcccttg caataaagca aggataccta atgaaagaaa
    23161 aagcgctttg ttttgcaaaa gaccttggtt caatgacggc ttggacaact ctctgcaatt
    23221 gatcatgtaa acagcttgag ctaagacgat attttgcaga agaatggttt gctgtaagct
    23281 ttgcccttca aactgcatag ccaataaata ggcaggaacc atgatcagta gggagacata
    23341 gatgatccga aacaaatgat aaccagatag gattccttgc tttggatctc ttggtgggcg
    23401 cttcatgatt tctggatctg gcgactcgaa ccctagttca taagataaag tgattgtcgt
    23461 taccatattg acccataaaa tctgtactgg ggacaaaggt agcgggcgat tcagcataag
    23521 tgcccaaatc aagatcaatc cttgagctaa tgcagttggc aagaagaaat tgatggtttt
    23581 ttgcaaatta tccaaaatcc gccggccctc ttttacggct tttgcaatcg tatggaaatt
    23641 atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat
    23701 agcaattcct acatcggatc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat
    23761 tccgacgatt tctccatttt tctgcaaagc tttaacaatt cgcagtttat gttctggtgt
    23821 cgttcgagca aagacatcga ctattggcac ttgctgaatc aactcttcat cagacataag
    23881 atccaaatcg atcccttcta aaactttggc agtatgtttt aagccaattt gttccgcaat
    23941 cgcttttgct gtgtctttgt gatcccctgt aatcattttc actgagatcc ctgcttgttg
    24001 gcattccttt acagctttga ctgcactttc ttttggcgga tcgatcaatc cggcgatccc
    24061 tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt
    24121 ataagcaaat cctaaaacac gctgaccttt tctcgcaaat tctgcagctg tctgactcca
    24181 tgcttcttgt tctgcagaag aaagagttga tttagcaaat aatacttctg gtgcaccttt
    24241 gacgtaaatc accgctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga
    24301 actgaacgga atctttgctt ctattttttt caaaggtaga ttggctgttt ctgtgtgtcg
    24361 aatcagcgca acttctgtcg gattgccact taaattttct gttttctgtt cttcttttgt
    24421 ttttaattcc tgacagtttt tcataatgga caacacgcag gcttcttctt ttgcagctac
    24481 atccacaact gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac agatcactgt
    24541 catagaacct aatgtttcta ccgaaggaag tcctttgatg atcgcctttt cttttgccat
    24601 ttcatgtact ccaacggata gaatcatggt caaaacagct ggcaatcctt tgggtaccat
    24661 tgacaccacc aaagcaatcg tagccgaaag aaggatatgc caatccatac catatcgaaa
    24721 tgtcgtaaaa ataactaaga ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt
    24781 taatcggttg atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt gcaatgcttg
    24841 attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc
    24901 agattgtac
//
LOCUS       DACEDM010000044        24860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEDM010000044 DACEDM010000000
VERSION     DACEDM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.044"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(125..895)
                     /locus_tag="IWO50_001641"
     CDS             complement(125..895)
                     /locus_tag="IWO50_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0358765.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(888..1823)
                     /locus_tag="IWO50_001642"
     CDS             complement(888..1823)
                     /locus_tag="IWO50_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358766.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2139..3788)
                     /locus_tag="IWO50_001643"
     CDS             complement(2139..3788)
                     /locus_tag="IWO50_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0358767.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(3905..6508)
                     /locus_tag="IWO50_001644"
     CDS             complement(3905..6508)
                     /locus_tag="IWO50_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0358768.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLILIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(6520..7569)
                     /locus_tag="IWO50_001645"
     CDS             complement(6520..7569)
                     /locus_tag="IWO50_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAQ0358769.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(7595..7909)
                     /locus_tag="IWO50_001646"
     CDS             complement(7595..7909)
                     /locus_tag="IWO50_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0358770.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(7925..8371)
                     /locus_tag="IWO50_001647"
     CDS             complement(7925..8371)
                     /locus_tag="IWO50_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0358771.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(8372..10348)
                     /locus_tag="IWO50_001648"
     CDS             complement(8372..10348)
                     /locus_tag="IWO50_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ0358772.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(10506..11402)
                     /locus_tag="IWO50_001649"
     CDS             complement(10506..11402)
                     /locus_tag="IWO50_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0358773.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(11395..12075)
                     /locus_tag="IWO50_001650"
     CDS             complement(11395..12075)
                     /locus_tag="IWO50_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0358774.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(12076..12858)
                     /locus_tag="IWO50_001651"
     CDS             complement(12076..12858)
                     /locus_tag="IWO50_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0358775.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12851..13768)
                     /locus_tag="IWO50_001652"
     CDS             complement(12851..13768)
                     /locus_tag="IWO50_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0358776.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13932..14255)
                     /locus_tag="IWO50_001653"
     CDS             complement(13932..14255)
                     /locus_tag="IWO50_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0358777.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            15043..18270
                     /gene="fss3"
                     /locus_tag="IWO50_001654"
     CDS             15043..18270
                     /gene="fss3"
                     /locus_tag="IWO50_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0358778.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18383..18697
                     /locus_tag="IWO50_001655"
     CDS             18383..18697
                     /locus_tag="IWO50_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0358779.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18710..19084
                     /locus_tag="IWO50_001656"
     CDS             18710..19084
                     /locus_tag="IWO50_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0358780.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            19094..19429
                     /locus_tag="IWO50_001657"
     CDS             19094..19429
                     /locus_tag="IWO50_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0358781.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            19512..20852
                     /locus_tag="IWO50_001658"
     CDS             19512..20852
                     /locus_tag="IWO50_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0358782.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            20930..21604
                     /locus_tag="IWO50_001659"
     CDS             20930..21604
                     /locus_tag="IWO50_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0358783.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            21837..22271
                     /gene="dcm"
                     /locus_tag="IWO50_001660"
     CDS             21837..22271
                     /gene="dcm"
                     /locus_tag="IWO50_001660"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0358784.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            22272..22979
                     /locus_tag="IWO50_001661"
     CDS             22272..22979
                     /locus_tag="IWO50_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0358785.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            22969..23259
                     /locus_tag="IWO50_001662"
     CDS             22969..23259
                     /locus_tag="IWO50_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358786.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            23518..24702
                     /locus_tag="IWO50_001663"
     CDS             23518..24702
                     /locus_tag="IWO50_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0358787.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            24717..24836
                     /locus_tag="IWO50_001664"
     CDS             24717..24836
                     /locus_tag="IWO50_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0358788.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         7736 a         4787 c         4639 g         7698 t
ORIGIN      
        1 cactaaacgg tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga
       61 ggaactattt tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
      121 ttttctaact tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa
      181 caacgatgaa tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa
      241 ggaatccgta acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt
      301 gccaaaatgg cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct
      361 gcaagacaaa tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga
      421 gcagtcctgc taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc
      481 atgaatacac atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga
      541 gaatactgcg acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag
      601 gcgctaccaa gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa
      661 cgttggcata cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca
      721 tatatgaaaa accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa
      781 agtacaagga tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc
      841 ggttcgtttc cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc
      901 tcctccgtat gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac
      961 ttattcctca tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc
     1021 accccttgat ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg
     1081 tatccttcaa tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat
     1141 tgcagtttgg atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg
     1201 atcccgatgt tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc
     1261 gttgtaccat tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata
     1321 tcgactcctg ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta
     1381 gcaatcatca gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt
     1441 tcccaaagat tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag
     1501 acaccgtagt agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa
     1561 ttgaactctt gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga
     1621 tcataaccga agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg
     1681 atcgttgtag acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc
     1741 acggaaagat cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta
     1801 tttatttcaa gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg
     1861 tatcatggaa tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg
     1921 gctcaggaaa atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga
     1981 aaaaacaaat cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca
     2041 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc
     2101 aagatttttg atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc
     2161 cgcttttcat caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa
     2221 tccaaaactt tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg
     2281 aaatcaaaac tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa
     2341 taattcttta aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga
     2401 tactccgcaa gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc
     2461 ccttttaaaa tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac
     2521 aaacgtttga tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct
     2581 tcgattgcca tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct
     2641 gtttggatcg tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt
     2701 cctgatgcat tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc
     2761 gcttctttta acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct
     2821 ggtcggcaag tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat
     2881 tctcccagca attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga
     2941 acgaaatgat aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac
     3001 tctgcttcac tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat
     3061 ggtttagttt gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg
     3121 gcggctgaaa tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt
     3181 ttcttcacta gataatagac cgtattgaat actaacccac cgattgccaa atagattcct
     3241 agtacttgtc ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata
     3301 aataaaccta cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa
     3361 ttatgatctt tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga
     3421 ttttcctgat aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg
     3481 cgtaaacgtt gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg
     3541 aatacttcga acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa
     3601 cggaacttct tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt
     3661 tgataaggaa ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa
     3721 atgtctaaag caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt
     3781 tgacccatat ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat
     3841 ttgaaaagag ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac
     3901 atatttatct ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt
     3961 cttgtttatt ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta
     4021 ttttttcttg ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag
     4081 aaggataaat cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag
     4141 gaacatcaag ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat
     4201 tcagtttctg cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca
     4261 ctttgaactc attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac
     4321 ctatctcttg agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg
     4381 aagctcgccc aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata
     4441 aggttatgat taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg
     4501 taaaataatg atcattttct gccacagaga cactcttatc gaatatttct aaattaacag
     4561 gtttctctac catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa
     4621 atccgttttg aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc
     4681 gcatacgatg cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt
     4741 ctcccagact gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac
     4801 gatcttttaa atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag
     4861 aatctttata tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg
     4921 gtgaataatc atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat
     4981 attgtctatc tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat
     5041 tgttacttat ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct
     5101 caaattcaac aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt
     5161 cataataagc tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt
     5221 tccgttctgg aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta
     5281 ttttctttga atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg
     5341 tgttgaaaac aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa
     5401 taaggttttc gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt
     5461 ccgcaacatt atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct
     5521 ttttccataa ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca
     5581 gtatgagcgg ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca
     5641 tttttgttct tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt
     5701 cacctttata ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat
     5761 ctgaaataat attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat
     5821 ttaaaatcat tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt
     5881 gcatatgaag catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt
     5941 taattgcgtc taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa
     6001 caaatttatc tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt
     6061 ttaatcctcg ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat
     6121 tcgcattgaa cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa
     6181 ggatcgtatc actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata
     6241 aggcatctgt ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt
     6301 ggataacctt aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc
     6361 catctaaaca aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga
     6421 cctgatcaga caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg
     6481 tgtgttgaat tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga
     6541 atttacttgt tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt
     6601 aaccactccc ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat
     6661 tgccataaag gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca
     6721 tgctactgca gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa
     6781 cgggatcgtc ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc
     6841 tcgttccgat tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg
     6901 cggaatagca attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga
     6961 agcaacccca aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat
     7021 tcctcctaaa atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt
     7081 agtcagtcct gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc
     7141 aacaactaat gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat
     7201 cggtactttt acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat
     7261 tcctccaata aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc
     7321 tagaccggga cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa
     7381 ctgtaatccc gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca
     7441 aacacctgtg ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag
     7501 cgggatcata taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt
     7561 gaattccatt agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt
     7621 catcaatcgt ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt
     7681 taccgtcaaa gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg
     7741 cctcggaaac atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta
     7801 ctttaatatc taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag
     7861 tatgggcaat ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct
     7921 ccttttaaaa tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt
     7981 taacaaactc gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag
     8041 tacctttatc agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta
     8101 atgcgcccca ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg
     8161 ctaggctttt gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt
     8221 ctctgtcaaa tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta
     8281 acaatttcga taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta
     8341 tattatttat attgattttt tcactgatca tttataagcc ttctttcaat atataaacta
     8401 atttttcaaa cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt
     8461 cattgaatgt ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa
     8521 agaaaacaga atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac
     8581 agattactgg cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg
     8641 ctgttgaaga tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat
     8701 tttcttttac caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt
     8761 ggtgggtaaa aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt
     8821 catttaactg tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa
     8881 caacaataac aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag
     8941 tgacttttct atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg
     9001 caaaaccgtt atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa
     9061 ctaatatcac tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg
     9121 ttacgtaagc gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta
     9181 ataaatctac acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag
     9241 ggttatagat atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta
     9301 gattattgat caattcttca tcaggataaa attcatttaa tttaattctt actaactctt
     9361 gtagctcaac acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag
     9421 catttttttc aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa
     9481 taattgaaaa tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt
     9541 cttcagaatt attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag
     9601 caaggtggat agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca
     9661 tgtatcgatt aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg
     9721 cttcaccaat gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa
     9781 agccacccca ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag
     9841 ctttgatgcg cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt
     9901 ttctaacatc tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa
     9961 gattttgaat ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac
    10021 ggtcctccgg agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt
    10081 ctttttttcc aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca
    10141 ttttttggat tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat
    10201 ataattgttc tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta
    10261 ttttcatttg ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta
    10321 agtgtacaat tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt
    10381 tctaatattc ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct
    10441 tttttctcta ttaaatatat ataagataat acatttttct actagttctc actaatcttg
    10501 tataattaaa ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg
    10561 gtcatctctc ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta
    10621 gattccgtat ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca
    10681 gaggtttgcg ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct
    10741 tttgaaaatg tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt
    10801 tgcagcactc gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca
    10861 gcgaacgcta cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct
    10921 tccacctctt tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt
    10981 cgagcaagat cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg
    11041 atcgttaaat acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa
    11101 ccgttaatag cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg
    11161 atgttttctt ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta
    11221 ttcagtgcat ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca
    11281 ccatattctt gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt
    11341 tgcaggaaga aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc
    11401 aaatctaaac cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt
    11461 ccctcgaaga ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg
    11521 ccaaactgtt tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag
    11581 cttcagcaga tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc
    11641 cctcgactct ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac
    11701 ttggacagaa gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc
    11761 aaatggtttc gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg
    11821 atcgcttttt gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa
    11881 caatgcttca cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata
    11941 tgtgcttaac aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg
    12001 ttcaagaacc tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt
    12061 ttccagcagg accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga
    12121 tgataccacg attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc
    12181 ttgttgcgca aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc
    12241 caagcctttg atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa
    12301 agaaagaagc aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga
    12361 taatgtccct aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata
    12421 gtttttgatt ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag
    12481 tcccgtgatc aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac
    12541 ataataagtc attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa
    12601 ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat
    12661 tttttctacc attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa
    12721 ttcattgccg tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac
    12781 gaccatcagt cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc
    12841 agttaataga ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt
    12901 tttgcccaac taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga
    12961 tggctgctac ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat
    13021 aatccctata accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa
    13081 tttttataaa ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt
    13141 gtccttcatg caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc
    13201 tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt
    13261 ctgccatccc tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat
    13321 tcgtgatcag tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg
    13381 ctttcttttt ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat
    13441 ccgacacacc gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat
    13501 aaagagcagg ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg
    13561 ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa
    13621 aggtagactt acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt
    13681 cgattgatac tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt
    13741 tcaagaccgt ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt
    13801 atagttgacg ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa
    13861 ttgaatgtca aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt
    13921 atacgcttgt atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc
    13981 tcttcactga aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt
    14041 ataaggataa ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt
    14101 tgcgttgtct tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc
    14161 atggtaatcg ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc
    14221 tgaagccaga ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa
    14281 aaataataca tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag
    14341 acttttcaca atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    14401 gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag
    14461 ctgaccactt ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat
    14521 ctttttcccg tttgtttata gatcgaatga tattatagac caagaatcct acctattaac
    14581 atagctaaaa acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga
    14641 ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg
    14701 aacgataatc gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct
    14761 ggttttttct tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg
    14821 taaatatatt ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta
    14881 atttaaaggc agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat
    14941 ccattctatt cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga
    15001 gcatcttatt tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa
    15061 aagaaattat gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt
    15121 gctcctacga ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta
    15181 gatttagcac atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa
    15241 gtcactgttc cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa
    15301 ccaggagtcc cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat
    15361 atgtccgata aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat
    15421 actcatattg tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca
    15481 ataaaacgat cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa
    15541 gccattgacg attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta
    15601 gggcaatcga ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt
    15661 gtggaaaatg ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca
    15721 aatgccaatt caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca
    15781 cctgtcgcct ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct
    15841 aatacctaca caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc
    15901 gataaagaat cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact
    15961 ttacctgcaa aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct
    16021 catggtacaa aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc
    16081 acccccatga ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca
    16141 cgggaaaaag ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg
    16201 aatgacaatt attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt
    16261 gaaatcgttc aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt
    16321 gctaatgcgt tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc
    16381 gtgaatacgt tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct
    16441 gttaccagta acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa
    16501 gaagacaaag atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac
    16561 accttgttta ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag
    16621 ttagcgaagg gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac
    16681 caagttgccg ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc
    16741 gaaggatata gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc
    16801 gaaaaaaatg ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc
    16861 tttgatttct ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat
    16921 ctgaccttta aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa
    16981 gcgaccacag cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt
    17041 ccttatgggg attatttact tgaagaagta gaagccccag aaggttttca aaagattacg
    17101 ccattagaaa tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat
    17161 gtctttacca ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac
    17221 gagaagctga cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg
    17281 cctgaggaag aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta
    17341 acttctctgg attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa
    17401 gaagactggt atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa
    17461 aaagatgaaa aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa
    17521 gagaaaacag gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa
    17581 actattgtct tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag
    17641 ccagtagcga aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa
    17701 caccatgttt ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat
    17761 ggtgatgtgg tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca
    17821 aaagaagctt tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt
    17881 tggaaatctg gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt
    17941 gcgaaaaaag tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc
    18001 aattacgata aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct
    18061 caaaccttaa caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca
    18121 ccaactgttc caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc
    18181 ggggagaaaa attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg
    18241 ggctattatt tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag
    18301 aaccaacgtg cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta
    18361 acaatgaaaa gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac
    18421 ctttggaaag ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa
    18481 tactacggtg attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat
    18541 tgaggtggta gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa
    18601 agccgtgaat ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga
    18661 ttacgtgttg tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac
    18721 agaaggtatt gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg
    18781 ccgagaagta cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac
    18841 ctataattta aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt
    18901 acccttacgc gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac
    18961 cgttgccaat tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga
    19021 tttagtcttg aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa
    19081 ataggtgaag attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg
    19141 aaaaggcgag ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt
    19201 tctgattata catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt
    19261 actagactgg ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt
    19321 atgtcggcag gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga
    19381 aggcttttta aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac
    19441 tagcataact ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg
    19501 aaaccatcaa aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc
    19561 actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc
    19621 aagagtttag acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt
    19681 ctataaaaga tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc
    19741 tttatattca cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc
    19801 ggatgattct agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca
    19861 aagatttgaa tagtagccga accagagaga ctatcagtta cttccccaaa atctattatc
    19921 gaatgaaaga tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc
    19981 agcttttaaa attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg
    20041 aactcaaaga ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg
    20101 ggatagacga agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg
    20161 cttatgattc tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt
    20221 tcctcctcac cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc
    20281 ccaaaaatgc agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg
    20341 aagaaatttc tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa
    20401 tgaaagaaat gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca
    20461 actttttaat ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg
    20521 cggtgatttt gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc
    20581 tgattttggc ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat
    20641 tcaactttcg cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg
    20701 aggtcgaaaa aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg
    20761 gcagtgtgat tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt
    20821 caattagtaa attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc
    20881 aagtaacgta taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa
    20941 tgattcttat ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa
    21001 agggaaatcc cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg
    21061 aaaatcagag gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc
    21121 ggattacttt cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa
    21181 agcatcttct tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt
    21241 tattctggat gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa
    21301 aaaacatttt cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt
    21361 tacacaagaa cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc
    21421 ttttttcaaa tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct
    21481 tcatatcata ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa
    21541 tctagaatta tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga
    21601 ctgagaaaga acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat
    21661 cggaaaatgc tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca
    21721 atgaaaaaca acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt
    21781 atgagccaag agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga
    21841 gatttttaga tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc
    21901 atcattgtat cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc
    21961 atgatacaag taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc
    22021 aatccctcgg accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg
    22081 cgggaaagcg gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt
    22141 tcaccactct tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc
    22201 acgaaggagg ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg
    22261 tggagtggta ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc
    22321 ttatcggaca ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt
    22381 caacatctga tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg
    22441 gtggacaagt ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca
    22501 aaatcgcact gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg
    22561 aacaaggtaa tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag
    22621 gtggtacaaa aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag
    22681 acagtgtgaa tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga
    22741 gtgttcatac cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc
    22801 ggaagctaac acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc
    22861 gagattctca agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag
    22921 ttccggtcat ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat
    22981 gagttgattg gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa
    23041 gaacaattgc gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat
    23101 ccatatttaa tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa
    23161 tttgagcgga ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg
    23221 aaagcagagt tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact
    23281 gttaggcgct gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt
    23341 catggacggc gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa
    23401 acacaaaaaa taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat
    23461 ttgtataggt gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg
    23521 gcacaacgga atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca
    23581 caaaataaat tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat
    23641 ggtggcgtaa ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt
    23701 aatcccgatt tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg
    23761 gatgcacgaa aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa
    23821 gattatgcct tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg
    23881 tctcatgaag aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt
    23941 gaaacttttt tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag
    24001 gccggtggcg tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat
    24061 atacctgagc taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt
    24121 aaaagttatc gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc
    24181 ttatatatcg gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa
    24241 caatatatca agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt
    24301 cgactgaaaa atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt
    24361 gagagcacca cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt
    24421 aaaaggcgaa ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg
    24481 aaagagattc aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg
    24541 ctaggccgac aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg
    24601 acaacctata tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt
    24661 ttggagcaac aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg
    24721 gaattgtaaa agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt
    24781 gcttgatgaa tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag
    24841 atgaatttcg gacaaaattt
//
LOCUS       DACEDM010000045        24528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEDM010000045 DACEDM010000000
VERSION     DACEDM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.045"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWO50_001665"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWO50_001665"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0358789.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IWO50_001666"
     CDS             complement(937..1518)
                     /locus_tag="IWO50_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0358790.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWO50_001667"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWO50_001667"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0358791.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IWO50_001668"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IWO50_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0358792.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IWO50_001669"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IWO50_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0358793.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IWO50_001670"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IWO50_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0358794.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IWO50_001671"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IWO50_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0358795.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWO50_001672"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWO50_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0358796.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IWO50_001673"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IWO50_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0358797.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IWO50_001674"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IWO50_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0358798.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IWO50_001675"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IWO50_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0358799.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IWO50_001676"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IWO50_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0358800.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IWO50_001677"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IWO50_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0358801.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IWO50_001678"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IWO50_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0358802.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IWO50_001679"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IWO50_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0358803.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IWO50_001680"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IWO50_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0358804.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IWO50_001681"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IWO50_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0358805.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWO50_001682"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWO50_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0358806.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IWO50_001683"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IWO50_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0358807.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IWO50_001684"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IWO50_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0358808.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IWO50_001685"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IWO50_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0358809.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IWO50_001686"
     CDS             13972..14157
                     /locus_tag="IWO50_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0358810.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IWO50_001687"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IWO50_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0358811.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IWO50_001688"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IWO50_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0358812.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IWO50_001689"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IWO50_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0358813.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IWO50_001690"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IWO50_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0358814.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IWO50_001691"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IWO50_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0358815.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IWO50_001692"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IWO50_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0358816.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IWO50_001693"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IWO50_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0358817.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IWO50_001694"
     CDS             18232..18879
                     /locus_tag="IWO50_001694"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0358818.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IWO50_001695"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IWO50_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0358819.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWO50_001696"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWO50_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0358820.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IWO50_001697"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IWO50_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0358821.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IWO50_001698"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IWO50_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0358822.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IWO50_001699"
     CDS             20317..21255
                     /locus_tag="IWO50_001699"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0358823.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IWO50_001700"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IWO50_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0358824.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IWO50_001701"
     CDS             21935..22774
                     /locus_tag="IWO50_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0358825.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IWO50_001702"
     CDS             22750..23619
                     /locus_tag="IWO50_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0358826.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IWO50_001703"
     CDS             23609..24406
                     /locus_tag="IWO50_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0358827.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT         7938 a         4546 c         5275 g         6769 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttctttt
//
LOCUS       DACEDM010000046        24132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEDM010000046 DACEDM010000000
VERSION     DACEDM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.046"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            107..754
                     /locus_tag="IWO50_001704"
     CDS             107..754
                     /locus_tag="IWO50_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358828.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(847..3126)
                     /locus_tag="IWO50_001705"
     CDS             complement(847..3126)
                     /locus_tag="IWO50_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0358829.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3404..5227
                     /gene="cadA"
                     /locus_tag="IWO50_001706"
     CDS             3404..5227
                     /gene="cadA"
                     /locus_tag="IWO50_001706"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0358830.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5274..5453
                     /locus_tag="IWO50_001707"
     CDS             5274..5453
                     /locus_tag="IWO50_001707"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0358831.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5790..7730
                     /gene="thrS"
                     /locus_tag="IWO50_001708"
     CDS             5790..7730
                     /gene="thrS"
                     /locus_tag="IWO50_001708"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0358832.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7954..8802
                     /locus_tag="IWO50_001709"
     CDS             7954..8802
                     /locus_tag="IWO50_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0358833.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            9096..11249
                     /locus_tag="IWO50_001710"
     CDS             9096..11249
                     /locus_tag="IWO50_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0358834.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTSAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(11435..12244)
                     /locus_tag="IWO50_001711"
     CDS             complement(11435..12244)
                     /locus_tag="IWO50_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0358835.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            12537..13973
                     /locus_tag="IWO50_001712"
     CDS             12537..13973
                     /locus_tag="IWO50_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0358836.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            14143..15339
                     /locus_tag="IWO50_001713"
     CDS             14143..15339
                     /locus_tag="IWO50_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0358837.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(15401..15586)
                     /locus_tag="IWO50_001714"
     CDS             complement(15401..15586)
                     /locus_tag="IWO50_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0358838.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            15817..19131
                     /gene="dnaE"
                     /locus_tag="IWO50_001715"
     CDS             15817..19131
                     /gene="dnaE"
                     /locus_tag="IWO50_001715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0358839.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            19299..20261
                     /gene="pfkA"
                     /locus_tag="IWO50_001716"
     CDS             19299..20261
                     /gene="pfkA"
                     /locus_tag="IWO50_001716"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0358840.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            20335..22119
                     /gene="pyk"
                     /locus_tag="IWO50_001717"
     CDS             20335..22119
                     /gene="pyk"
                     /locus_tag="IWO50_001717"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0358841.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(22154..23056)
                     /locus_tag="IWO50_001718"
     CDS             complement(22154..23056)
                     /locus_tag="IWO50_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0358842.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            23242..23811
                     /locus_tag="IWO50_001719"
     CDS             23242..23811
                     /locus_tag="IWO50_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0358843.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            23869..24048
                     /gene="rpmF"
                     /locus_tag="IWO50_001720"
     CDS             23869..24048
                     /gene="rpmF"
                     /locus_tag="IWO50_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0358844.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
BASE COUNT         7675 a         4263 c         5106 g         7088 t
ORIGIN      
        1 ttttttgtgt gatttatttt cttttctaat tcaacctgtg gtatgatgag agcataagta
       61 tcatgtgaaa tgatatgaag tatgtggaag atggacgggg aagaatatgg aagcaaaaat
      121 cactttagaa ccttttgaac gtatcttaag cggttaccgc aaagtagaag agttagctgt
      181 gaacgtaact gattgttcaa aattagcgca aaaatacgca agattcggcg ttgaaggcta
      241 tcgattagga aactatgtcg gtacagggta tctgaaccgt tatttagagt gtatggtgga
      301 ccgcgcacca atgctgattt atagacaaaa ttatctgatt ccgctgcttt ttcgtcgttc
      361 tgattcagcc tttcggttat ttgaagaaga gtatcggatg gaggctttct ttctactatt
      421 agagtggagc ttgaaacatc gtcctgaaaa aattcttatc gaaagaaatg agaagataga
      481 cacgaaaaaa aataaagtga tcgatagtgc atacctagca tttcgtgtat cagagatttt
      541 ggattgtggc ggctatccaa tcagcaattt ccaatcaatt gaccaattca ttgaatggaa
      601 tcgaatttac cgattgatcg acaatggcgg gattggccgt catagcaagg tatttgaccc
      661 agaatatcca gaaaacatgg aagaactgaa aatgattatt tcgcttgtca aattaaaata
      721 tcctgaaaca gatttggatt tatatataga ataaatgacc ttaaatgatt gaattacttt
      781 ttctgcaatt attattgtaa ataaaaagac aactgctaac attaatgata gcagttgtct
      841 ttttacttac agtacttttt tctcataaga tgaaatatcc atattgttta acaaaggact
      901 ttcattattg gaggtaaaaa gggtcaattc atgcatcgca cgtgtacaca tggtgtaaag
      961 gatcaatttg tcttgctgag aagaaaaatg ttctgcatct acatcccaca agaaaactcg
     1021 gtcaaactcc agaccttttg ctaaaaaggc tggaagtaaa ataatggaac gcttcataaa
     1081 gtcactctca tttaaaatca gctggacttg ttgtttcaac tcgtctggca gttgagcata
     1141 aagagcttcg cattcttctt gtgttttagc tatgattgct gtacgccaat accgggcttt
     1201 tccctgagtc gttcgaatgg tatctgctaa ccaattcaat ttgatatcat ctgttgttcc
     1261 ttggatgagc aaaggaaggt ctccttttct tgctgttgtt tctacttgat gttctttcac
     1321 aataaaatga ttagcaaaat cagtgatttc ttttgtggag cgatagcttg tcgttaattg
     1381 gaaacgagtg acttcacggt ccacgaaaag gtcttccaat gatccaacga tcgtttcatt
     1441 tccaaagaca ttttgattta gatctccaca gaaagtcaaa tttgcttttg ggaacaaggc
     1501 ttgcagcaat gccacttgtg atggtgcgaa atcctgcatt tcatcaataa agatatagcg
     1561 agctttgtgt gtaaccgtca ctggatatgt tcggcgcatc agtaaaaaga atagtacagc
     1621 atcctcttga cgcaattctc tttgacgcaa gtgtgtacga acagttgtca aatgttcctg
     1681 ccacctggtc tcgctgattt tatgtcgtgt tagaagagaa agtggcacag tttgtagaaa
     1741 gtggagatat tgtttcgtta tattgacaaa ttgataattc atgatccctc gatggattcg
     1801 acggaatctt ttacgtacga tttggttggc cagtttttta cgtaatgaac gctccttttg
     1861 ttctgtatat tcaagatttg gatctgttgc gtatagttct tggagctgtt cttccgccag
     1921 atctttgacc cattgctgac gtgtttcgtc tttttgcaga ccgcctaatt ttttcaggag
     1981 cttcgtttgt aatagagaaa gtctatgatg taaaggtaat tgttcatttg tttctttata
     2041 ccattggcga atcgattcct ttgataggat cgtacggcca tttattttca tatcacggaa
     2101 caagggaccg tagtttgtga tgccttggat atagcgatca atctgatcga ctaaggtcaa
     2161 tccgcttttc atcacttgta tcggatcttt ttcccctagc aaaaaaccac tttcttgttg
     2221 ctgttcatct agtaaagaaa actctggcag cagctgcacc aaataatttt tgaatgtctg
     2281 tgtaggcacg ccgctttcac ctaatgaagg caatacagtc gagatgtaat cagaaaacaa
     2341 atggttgggt gaaaatagaa gaacgttttc agcctcgagc cacttgcgat tatgatacaa
     2401 cagaaaagca atacgttgaa gcaaagcgga tgtctttcca cttccggcga ttccttcaat
     2461 cagcattact ttacttgttg tgtcccgaat gattgcattt tgtgcttttt gtatcgtaga
     2521 gacgatgttt ttcatatggt tgcttgatga ttcatccaat atctctagca aaaagtcatc
     2581 attgattact tccgacgtat ctaccataga taaaatacgt ccttcttgga ttttaaattg
     2641 tcgtttcaac tgcagatcga catctattct atctgtttca gtttcataaa atgcgggacc
     2701 aatctcacct tcataataaa gattggcaat tggtgcgcgc cagtcaataa cgattgtttc
     2761 ttctgccaga tctcttaaag aagcgatacc tagatataat gtttctgttt cacggtcttc
     2821 tttaaagtcg atacgggcaa aataaggact atctttcatg acagacaacg tttgcaggcg
     2881 tttttgctgt gcttcaagag tatggtattt caaaagcaat tcctgctcat gttggcgata
     2941 ttcaacagca gattcataaa aagcttcatt ggaaccagct cgaatcttgt gattagctgt
     3001 gtcttttgtc gaatgattca tttcctgagt caattcttgt tgttttgtat ccagcatatt
     3061 ttgttcgatc tggattagtt cgatcgtttc atccacgtgc gtttgttcta gttgtttctc
     3121 attcatcgca tgtttgctcc ttccgccgta aataacgaat taaaagtata gcatagtttt
     3181 tcgaaaaaag gtacttttta actgatgaat cgaaaaagta atccttgctg aatatttttg
     3241 tttgtagaac tgatgaattt tggaaacgct gatagtgata gttgttttca attaaggcct
     3301 attgttttaa aaagtgctag tcaaggacta tgcttaagat gaaagaggca gctggaaatt
     3361 tattactaac ctctctaagt cacataaaag aggagaaaaa acaatgacac atttcaaaaa
     3421 atttttactt actttgctta ttggtgggat agctttatta agtgaatttg tttttcacca
     3481 gccggccctt gcatttgcga ttgtcgcatt tactggaggg attctcgcct tcttgatgtt
     3541 tttggaaatg atcaagacgt tacgttcagg aagatatggc gtagatgtat tagcgattac
     3601 cgcaatcctt gcaacattag ccgtcaaaga atactgggct agtttgatga tattgatcat
     3661 gcttacgggc ggagatagtc tagaggatta cgcgaataaa cgagcgggta aagaactgca
     3721 gtctttgtta gataacaccc caagaattgc ccatcgattc aaagaaggaa aattgcagga
     3781 tatagtaatc gatgaaataa atattgatga ccagttacta gtcaagcctg gcgaacttgt
     3841 acccgcagat ggtcgagtga ccaaaggaga atcttcggtc gatgaatctt ctttgacagg
     3901 tgagtcccgc ccggaaacca agaaacctgg agatcagatt atgtcagggt ctttaaatgg
     3961 agacggatcg atccaaatga tcgctgaaaa acgtgccgca gacagccagt atcaaatgat
     4021 tattaaacta gtcgaagaat caaaagaaaa gcctgcccga tttgtccgtt tagctgatcg
     4081 ttatgcagtc cctttcacgt tgattgctta tttgatcggt ggtatttctt ggtggttctc
     4141 aaaagaccct ttacgatttg ctgaggtact ggtagtggca tcaccttgtc cactgattct
     4201 agctgcgccc attgctttag ttgcaggaat gagccgttca agtaaaaacg ggatcgttgt
     4261 aaaaactgga acaacgattg aaaagctcgc acgtacaaaa acgattgctt ttgataaaac
     4321 aggaacgctt accaaaggga tattggaagt agaggcgatt cagccagtca atggaataaa
     4381 aaaagaagag ctacttctgt atgcagctag tgcagaacag gaatctagcc atatcttagc
     4441 tcgttcgtta gtgaacaacg tgaaaaacag ccaactgtat cctgttagtc aactgaaaga
     4501 agtgaccggt caaggaatcc aagcagtagt caataatcat gaagtaaaga ttggacgtgc
     4561 ctcttttgtt tcagctgaag aaatagaaac gtccaataca gcaatctatg taacaattga
     4621 tcgatctttt gcaggaacga tcctattttc ggatcagatt cgcccagaag cagcaaagac
     4681 aatcaaggag ctaagaaaac ttgatgtgaa aaatttgatg atgatcaccg gggatggatc
     4741 tgccattgct caaacgattg cagaaaaagt ccaattagat tctgtacatg cccgtagctt
     4801 gcctcaagat aaactgacca ttttagaaag tatccctaaa gatcaaagac ctgttgcgat
     4861 ggtaggagac ggcgtgaatg acgcaccagc acttgctgct gcagatatcg gtattgcaat
     4921 gggggctcat ggttctacag ctgctagtga aagtgcagat gcggtcatct tgaaagatga
     4981 tctcttgcgt gtagcaaaag cagtagtgat ttcaagagat acgatgagaa tagcgaaaca
     5041 gtctgtactg attggcatct ttatttgtat ttttttgatg ttaattgcaa gtacgggact
     5101 aatccccgca ttattcggag ctgttttgca agaagtcgtg gatacggtct ctattttgag
     5161 tgccttgaga gcaaaaaaag atccagccat ttttcctgat tcagtaccca agacaaacat
     5221 agtatgatag aatcgagttg aaaaaagaac gatcagaaag aggagagagc attatgccat
     5281 tcgtacatgt cgaattagta gaaggtcgca gtccggaaca attagaaaat atgatgaaag
     5341 atatcacgga agctgttcat aaaaacacgc aggcaccaaa agaacacatc catgtcatca
     5401 tcaatgagat gaaaaaagga acatacggtg taaatgggga atggaaaaaa taagtttagt
     5461 ttcccacttg atttttctat ttattagtgg ttaaataaac acataattga ttgatgagaa
     5521 agccaactga atttgagagt cttcgataag agaggaaaag ctaggctgaa acttttccag
     5581 gagaaaacca aaggatgacc actttcgaaa cccttgttga agcaagggcg gtcggaccgt
     5641 tacaacgctt gaggaacgca tttgcgtttg aacattaggt ggaaccacga acttttcgtc
     5701 ctagtatctc ttttcaagag atgctaggat ttttttgttt ttcagcttga tgatctgatc
     5761 aatcaaaaca tttcaaggag gaaactatta tgataaaaat tacttttcca gatggtgcag
     5821 tcaaagaatt tgagtctgga acaacaacat tggcaattgc tgaaagcatt tcaaaaagct
     5881 tagcaaaaaa agctttagct ggtaaagtga acggaaaact cgttgatttg actcgtccaa
     5941 tcgaggaaga tgcaagtatt gagatcatca caccagacca tgaagatgca cttggcttag
     6001 tccgtcattc agcagctcat ttgatggccc aagccatgcg tcgcctctat ccaaatatcc
     6061 actttggcgt tggaccagct atcgattcag ggttttacta cgatacagat aatggacaaa
     6121 accaagtaac tgcagaagat ttgccggcaa tcgaagcaga aatgatgaaa atcgtcaaag
     6181 aaaatctgcc gatcgaacgc cgtgttcttt caaaacagga agcattagaa atttttgcaa
     6241 gtgatccgta caaagtagaa ctgattagtg aattgccaga agaagaagtg attactgctt
     6301 atcaacaagg cgaattcatc gatttgtgtc gtggaccaca tgttccatcc actggccgta
     6361 tacaagtatt taaattatta tcagtggcag gtgcttattg gcgtggaaac tcaaacaatc
     6421 aaatgatgca acgtgtatac gggacagcct tttttgacaa gaaagctttg aaagaatata
     6481 tccgtctccg tgaagaagca aaagaaagag accatcgtaa actaggaaaa gaacttgact
     6541 tgttcatggt ttccccagaa gtaggttctg gtttgccatt ctggcttcca aaaggggcaa
     6601 caatccgacg cacgatcgaa cgttatatcg tagacaaaga agttagtcta ggttaccaac
     6661 atgtatatac accaataatg ggagatgttg agttgtacaa aacatctgga cactgggatc
     6721 attatcaaga agacatgttc ccgccaatgg atatgggaga tggcgaaatg cttgttcttc
     6781 gcccaatgaa ctgccctcat cacatgatgg tctacaaaaa cacgatccat tcttatcgtg
     6841 aattaccaat tcgtatcgct gaattaggaa tgatgcatcg ctatgaaaaa tcaggtgctt
     6901 tgtcaggttt acaacgtgta cgagaaatga cgttgaatga tggtcataca tttgttcgtc
     6961 ctgatcaaat caaagatgaa tttaaacgta cgcttgaatt gatggtggca gtatatgctg
     7021 acttcaatat tacagattat cgtttccgct tgagctatcg cgatccaaac aatacagata
     7081 aatattttga tgatgacgca atgtgggaaa aagcccaagc aatgctgaaa gccgctatgg
     7141 atgaattaga actagattac tttgaagcag aaggcgaggc agccttttac ggaccaaaac
     7201 tagatgttca agtcaaaaca gctcttggta cagaagaaac attatcaact atacaattag
     7261 acttcttatt gccagagcgc tttgatttga catatgttgg agaagacggg gaaaatacgc
     7321 atcgtccagt agttatccac cgcggaattg tttctacaat ggaacgtttt gtcgcttatt
     7381 taacagaagt atacaaaggc gcttttccaa catggttggc acctatacaa gcaacaatca
     7441 tccctgtttc tgttgatgca catggcgatt atgcttatga aatcaaagaa cgtttgcaaa
     7501 tgaaggggtt gcgtgtagaa gttgatgatc gtaatgaaaa aatgggctat aagatacgtg
     7561 cctctcaaac acaaaagatc ccttaccaat tagttgttgg agacaaggaa ttggaagatg
     7621 caacagtgaa cgtacgtcgt tatggaagta aagaaacagc tgtagaagat ttgaatatct
     7681 ttattgatgc tatggaagca gaagtcaaaa attacagcag agaaaactaa taggtaaatt
     7741 tcctgtaaaa aagtagtaga tagaaagcaa tccgaacaat agataacagg tattcctcaa
     7801 agacacagat gctcaataga atctgtggga ttgatgggat aagatattct gttgggcgga
     7861 ttgttttttt gttttttatc cagtatacga ccgtgaataa gggtctgata tggtatactt
     7921 acatagacta tcattaaaag ggtggaggaa cttatgagcg taattagtcg tattgaaagt
     7981 atgatggaag attattcttc agcagaaaaa aagttggcca actacattat cggtaatatt
     8041 gagaaggtcc ctacaatgac agctaatgaa ttagcagaag cttccgggtt gagtgcgccg
     8101 acagttgtac gtttttccaa aaaaatcggt tttcaaagtc tgacggactt caaaatcacg
     8161 atttcaacag aactacagac tggaatcgat gaaggtttta gtgatataga agcaaatgag
     8221 tccttttact ctctcaaaaa caaacttggt aacaatgcac aagtagcgat aaaagaaaca
     8281 gtagatattc tagaagaaga aacgatccaa aaagtcgtag agtgtttgga atcggcagag
     8341 actgtttttt tatatggggt tggcgcctct tctttagtag tagaagatat tctgcaaaaa
     8401 tggtctcgtg tcggtaagcc aatcattttt gaaaaagata tccacgtctt gctcccgcaa
     8461 cttgtcagca atgagaaaaa gaaagtgctt tggcttgttt caaactcagg tagaagtgca
     8521 gatgtagtcg cattagccga gctggcgaaa agcatgaata tcgaaatcat tgcgttgacg
     8581 caatttggaa ataacccgct tagcaagata gccgatgtac tcgtacaaac atctagacca
     8641 aaagaaatca cgaatcgaag tgcggcaact aattcacttt tggcccaatt tgctacgatc
     8701 gatatcattt tttatttcta tatggctaaa aacgaaaagc ttagcgaaaa agtgaaaaaa
     8761 acacgagaag caatccaaga atattttatg gattcgaaat aaaaacctta ctatttttta
     8821 tataagtttt ttagaataac aaaacgtagt tttccacaaa taaatgatcc atcaaagtgt
     8881 ttcacgactt aagaaaacat aattttcaaa aataagttta ttttttaatg aaaaaaatag
     8941 ttggatatgt aactttaagt gattgctgtg aaacaaaaag ttgattttat cttgaaatta
     9001 ggtaaagaat ttcttgctcc gcccattttt tcttttttat taagaagttt ttgggtacaa
     9061 tgaagcatga atgtaaatag gcggaaggat aggaaatgat gaaagatttt atgaaaaaaa
     9121 tattaaatac ttcttggtta gaaaaaataa gaaaaggtgc acaagaacct aagaacaaaa
     9181 cgtttcgccg gtcccatgtt ccttttcgct taaattttct ttttttcatt atatttacgt
     9241 tatttgttgc gttgattgct agattgggtt atttgcagat cgtgaacggc gaagagatgg
     9301 aggcgcgagt gaagtctact tctacgatta cggtccaaga aagtactcct cgcggaatga
     9361 tctacgactc cacaggaaaa gctttagtta cgaataaagc gaatcaggcc atcacatata
     9421 cgagaggaac acaagttacg gctaaagatt tgcttgaaat cgcaacgaag ctgaactcgc
     9481 tgatcgatgt tccggtagat gaaaatctga cagatagaga taaaaaagat ttttggctgg
     9541 ctgatcccaa acatttgaaa gaagccacaa aaagactttc ggcaaaagaa aagcaattag
     9601 ggaacagcga acaatatagt gcgatggtca ataaagtcac agctgatgag attaatttaa
     9661 acgaggagca attaaaaatt gctaccatct ttaaacggat gaatagtgca tatgaattaa
     9721 acactgtcta tatcaaaaac tctggtgtta ctgatcaaga gttagctgtt gtggcagaac
     9781 gcgcttctga acttccagga gtttcgactg gcacagactg gacacgagaa tataatgcag
     9841 caagttcact gaaaagtatt cttggcagtg tgacgactga aaagcaagga cttccagcag
     9901 atgaagcaga gaaataccta gcaaaaggtt attctcggaa tgaccgtgta ggacaaagtt
     9961 atttggaaaa acaatatgaa gatgtcctgc aaggaaccaa aacccaatat gaagtttccc
    10021 ttgataatga aggaaatgtt tcgaatcaaa aagaaatctt ctctggggaa aaaggttcta
    10081 atttaatgtt atctatgaat gcagaattcc aatcaaaagt agaagaaatt ttgaaaagga
    10141 attatcaaac attgatcaac aacggcaaag cacagtattc gcctggtgct tatgcagtgg
    10201 ctatgaatcc tcagacagga gaagtgctag caatgactgg tttcagtcat gaacaaggtt
    10261 ccaaagaaat caccgagaat gctttaggca cgattactag cgcctttgat ccagggtctg
    10321 tagtcaaggc tggtactttg acagcaggct gggcaagtaa cgcgatcagc ggaaatcaag
    10381 ttttgattga tgagccgatt cgtctgcaag gtgcttcaga aaaaagctct gtctttaatc
    10441 gaagcggaca agttgctctt gatgcagtca aagcatcgga attgtcttct aatacgtata
    10501 tgatcaaagt ggcgttaaag atgttaggac tagactatac acctggaatg ggactaccta
    10561 gtttagatga agaagctaaa gcttatcagc aattgagaga ctctttcaaa gagtttggtc
    10621 ttggtacaac gactgggatc gatttgccga atgaatctcc tgggatctca cgttctgttg
    10681 attatatgaa gaagttcaat gcggataatg gcaaagaatg gtatgctcca ggtaacttta
    10741 ccgatttggc atttggacag tttgacacat atacaccaat ccaattagct caatatgctt
    10801 caacagtcgc taatggcggg aaacgagtac agccgcacct agtcaaagcg atctatggca
    10861 atgacgaaaa tggaaacttg ggtgaagtga agaaagaaat tggcacaaca gtagaaaata
    10921 cggtcaatat ttctgcagaa aatatggcga tcctccgtga aggattccac caagttgtcc
    10981 atggtaccga tccttacaca tcagccaaac cattagccag tgcgaaaatg gacctttccg
    11041 caaaaacagg gactgcagaa acggttgcgg aaggtcatcc agatattgcg acagtcaata
    11101 gtaatatcgt ggcatacgga ccaacagaca atccggaaat tgccatcagt gttgtcttgc
    11161 cgaacttatt agacgaacaa gaccacataa acttgacgat tgctaaagaa ataatggacg
    11221 cgtattatga tatgttcatg gcaaaataaa aaagaggtcg tgaaaaagcc gcttagtttc
    11281 gagtatgaag aaaaattttg tgaaaatagc ttctcacatt tttgcttaaa tttgacttca
    11341 taccgagaaa ctggcttttg aacaccgttt actcggcaaa aaaggttgtg acaaaaattg
    11401 tcacaacctc ttttttgtgg aatctaattc tctattatag tttaagtagt ttttccagat
    11461 aaacagctaa accgtcttcc tgattgctca aaggagtgac atcatttgca atgcctttga
    11521 gttggtcagt accgttagcc atagctactc cccaacctgc ataatccaac aattccaaat
    11581 cattatgctc gtcaccaaag gcaatgacgt ctttttgatc gatagacagg tagttagcaa
    11641 tctcttgcac gccttttgct ttttggattc cttttgaaac aatctctaag atggcagtag
    11701 gtccacccca tgtccgaaca tcgacataac tgccaaattg acgtgttaat tcagcagata
    11761 cagctcctgc aaaacgtttg tctgttcgga caagcaaaga tgtaggattt gtcgtcaaat
    11821 ttttaggaga aagcaaattc ttctctcctg gacgagaaga agcaaaaatt ttttcatcaa
    11881 agaaatcaaa actgtcaata aaaaaagtat cacgattttc agcagcgata aaatctaaat
    11941 ttagctgctt tttttgcgat aagatttcaa aagcgatttc acgattgatc aacgtttctc
    12001 tttcaccatc ccagtgctga ttagggatgt gtactaatgc gccgttaaaa ttaaccatcg
    12061 gtgttgttaa ttctaattgt tggtaaaact gatggctcat tcggaatggg cgtcctgtag
    12121 caatactgac atgatgaccg gcattgattg ctttttttag agtctctttt gttttagatg
    12181 taatcatcga atcttgattg agggtagttc cgtccagatc gatggcaatt aattttttat
    12241 tcaataaggg tccttccttc ctcaactagc atttgtttta gtctagcata ttttaggtaa
    12301 ttaacaagag caatcagcta ttcctaacaa aaactacagt tatggctata taaatagcta
    12361 aacataagct aaacacgaac gttaagtatt attttcgctt tttttgttaa aaaaacgttg
    12421 aaaagcgaat gaaacgtcta tataatttga aattgttcga tggatttgcc ctattttact
    12481 tattttttat tattatagtt ttccatcaga aaaaaattca gggagtggaa ctttttatgg
    12541 aaaagttttt cggtttaaag aaaaacaata caaatgtatc aactgaaata atggcgggag
    12601 taacgacatt ctttgccatg agttatatct tattcgtaaa tccgacgatt ttgtcagcat
    12661 caggtatgcc cttccaagca gtatttctag cgacaatcat tgcatcgatt attggtacgc
    12721 tggtaatggg attgttcgca aatgtaccat atgctcaggc acctggtatg ggattgaacg
    12781 catttttcac ttttacggtc gtttttgggc ttggttattc ttggcaacaa gccttggcaa
    12841 tggtgtttat ttgcggatta atcaatattt ttattactgt cacaaatatt cgaaaaatga
    12901 ttattcgggc gattcccgaa agtcttcaac atgcgattgg tggcgggatc ggtattttcg
    12961 ttgcgtatgt cggaatcaaa aatgcaggat tcttatcttt ttcagcagac cagtcagcta
    13021 tttcgagctc tgtcgttgaa ggcggaaaag cgacaaatgt tacgataaat ggtggaatcg
    13081 tccctgcgtt agccaacttt gacaacgctc ctatcttatt ggctgtgatt ggtttggtac
    13141 tgacagccat tttagttgta aaaaatgtcc gtggggctat tcttatcggt atcgtagcga
    13201 ccacagtcct tggtattttt atgggtgttg ttgatttaag ttccattgat tggcaaacga
    13261 attcgttagg taattcattt aaagaattag gaacaacttt cggtgcagca tttggttctg
    13321 aaggcatgca atctttattc agtgattctt ccaagattcc tcaagtattg atgacgatca
    13381 tcgcattcag tttatctgat actttcgata cgatcggaac atttatcggt acaggacgcc
    13441 gcacaggtat tttctcaaaa gaagacgaga tcgcgttgga agatggtcgt gggttcaaaa
    13501 caaaaatgga taaagcacta tttgccgatg cgatcgctac ttctgtgggg gctatctttg
    13561 gtacatcgaa tacaacaact tatgtcgaat ctgcagctgg gatcggggcc ggtggacgta
    13621 ctggtttgac ttcagtagta gtggcagtgc tattcgcatt aagtagttta ttctctccac
    13681 tgatcgccat tgtaccaaat caagcaactg cgccagcatt gattttagtt ggagtaatga
    13741 tgatggcttc ttttgcagac atcaaatggc tagatatgga agaagcttta ccggccttct
    13801 ttgcatcgat ctttatggga ttgtgctata gcatttccta tgggattgca gcaggattta
    13861 ttttctatac aattgtgaaa gtaatcaaag gaaaatacaa tcaagtttct gttgctttgt
    13921 ggatcgttga tattttattc attatcaact tcatcattct agcaacaatt taactaaaga
    13981 gaagggattg gaacagtatt tgttctgatc tctttttgtt ttacataaaa agaacgttac
    14041 atataaaatg acctttgcgg gatttttctc tttttactgg cgagatcgtt gcttgtttat
    14101 tcgaaaatag gtatgataaa agagacgaaa gagggatgat agatggagat taattggaga
    14161 aaaaatttag tgattgcgtg gattggctgt tttttcactg gtgcaagtat tagtttagtt
    14221 atgccattca taccagttta tgttgagcaa ctaggaacac caaaagatca aatcgaattg
    14281 ttttcgggac tagcaatctc tgtgactgct tttgcatctg ccgtggttgc accaatctgg
    14341 ggaaatctag ctgatcgaaa aggccgaaaa ctcatgatga tccgtgcagc agcagggatg
    14401 acattaacaa tgggatcttt ggcttttgta ccaaacgcgt attggctttt gatcatgcgg
    14461 ttttggaatg ggattttgtc tggatatatt ccaaatgcca cagcgatgat tgcttcacag
    14521 gcacctagag aaaagagcgg atgggcgtta ggaacattgt ctaccggagc gattgctgga
    14581 aatctgatcg gaccttcaat gggcggtgca ctggctcagt ggtttggtat ggaaaatgta
    14641 ttcttgatca caggggcttt gttgatgatc acaacggtat tgaccatttt cttagtcaaa
    14701 gaagatttcc atccaatcga gaaaaaagat ctgataagca ccaaagaaat tttttcaaaa
    14761 atggatcatc tctctatttt gatcggtctc ttcattacga ccttgatttt acagatagga
    14821 atcactacga tcagccctat tttgacctta tatattcgtg aattgagcgg aagtacagaa
    14881 aatatccttt ttgtcagcgg cttgattgtt tcgatagctg gtgtttccgc tgtcttttct
    14941 tcaccgaaac taggaaaact aggggataag attgggaatc aaaaagttct aattggagga
    15001 ctcattttat cattttgctg ttatttacct atggcattcg tcactacacc gctccaatta
    15061 ggtatacttc gctttatttt aggcttttca actggcgctt tgatgccttc tgtcaataca
    15121 ctgatcagta agattacgcc tcaagaagga gtcagccgaa tctatagcta caatcagatg
    15181 tttagcaact ttggacaagt tttaggtccc atgctaggat cgacagtcgc gcatgcttat
    15241 aattacagtg ctgtttttat tgtgacaagt ttgtttgttc tgtcaaatat tcttctttct
    15301 ttattcaact ttcgtaaagt tcttcgcagc agattataat ggtggtgagg agtagaaaat
    15361 aaaaaagagc ttgagaaatc tcaagctctt ggctgttttt ttacgaatgg gcagaggttt
    15421 cttttcttgg cgcaactgca ccaatcaaaa ggataatgac acccgcaatg aatgaaatga
    15481 ttgttgtcaa agtaaaatca tatgttccgc ttgttaatgc agcaccaata taaccgacta
    15541 cttgacctag aacgacagcc cagaataatg taacgatata tcgcataaag aacacctctt
    15601 ttctatctta attttaccag attttctttg aatgtaaaga aacgagacca aatctcaata
    15661 aaatttgagt tcttgcgaaa gaactcattc ctacagatta caagcaagca aatgaagtgg
    15721 aataatcaac ttcagcgcct gctatcttaa tgaaagtaaa aggatcaaac cttttaacgc
    15781 tagtgaattt taacgaaaaa aaaggcggaa tgcaagatgt ttttcccaca actttataca
    15841 attacttctt attctctttt acaaagtacg atacgtattc gagaatatgt acaagaagca
    15901 aaaaaacaag gctacacttc attagccata acggataaag atgtcttata cggtgcacct
    15961 gaattctatc gtatttgtgt agaagaagga gtacagccaa tcattggatt acatcttagt
    16021 tatacttatg aagaaaaaaa ttatggcttg cttgcgtacg caaaagatga aataggttat
    16081 cagcagctga tgaaactttc cagccgaaaa atgatccaag aaaaattgga acttgcagaa
    16141 atgacagaaa ccgaccatct agcgtttgtt gttccggcaa atcattctgt ttctgaggct
    16201 ttcaataaag aggataactt tttcggtcat tggcagtttt tacttgaaaa tgtttttact
    16261 agaaattttt atatcggctt gacagaaaac acgcctgatc aattgcctga atggtatcag
    16321 tatgtgataa aagaaaatta ttcgctttgt gcattacatc ccatcgctag tttacaacaa
    16381 gaagaacttt ttgcacttga agtgatgaaa catatcaaag agggaagcca aataccttta
    16441 gaaaatttat ctgcatcttt accactaaca gcaaatgtct atcttctttc agaacaagaa
    16501 atgatcaaac gattcgaaaa agcgggtctt tcccaagctg taataggcgc ccagcgttta
    16561 gctgaatcga tttcatttga attccagttc catcagaaat tacttccgca ttatccagtt
    16621 cctaatggaa aacaagctgg agaatattta agagagttat gctattcgca agtggaacgc
    16681 cgaatcactg actatacaaa ggaataccaa gaacgtttgg actacgagtt atctgtcatc
    16741 catcaaatgg gattcgatga ttatttcttg atcgtatggg atgtaatggc ttttgctcat
    16801 agaaacaaaa tcgttaccgg tgcaggtcgt ggttctgctg cgggttcatt agtagcctat
    16861 gtgttagaaa tcacagatgt ggatccatta gaatatgatt tgctgttcga acgcttttta
    16921 aaccccgaac gctttacgat gccagatatc gatttggata ttcctgacaa tcgacgtgaa
    16981 gaagtattga attatgttcg agaaaagtat ggacaatatc atatggcaca aatcgcaaca
    17041 tttggcacaa tggcagcaaa aatggttctt agagatgcag ctagagtgtt tggtttatcg
    17101 caaagtgaag caaaccgctg gtcaaaagca atacccaatc aattgaaaat cacattgacc
    17161 caagcttata aaacttcgca tcaattagta gaattagttc aggcaagcga aaaaaaccaa
    17221 cttttattca aaactgcagt gattttagaa ggattgccaa gacatgtttc gactcacgca
    17281 gcaggtgtag ttatcagcga taagaatcta cttgaactag ttccgctcca acgcggttct
    17341 gatgaagctt ttctgaccca gtatacgatg aatgaggtag aagcaatcgg tcttttgaaa
    17401 atggactttt taggactacg caacttatcg attattgatt ttactttgaa agctgttaag
    17461 cgagtagaag ggtatgagat ccgattaaaa gagatcccgc taaatgatgc aaaaacgctg
    17521 ctcttgtttc aacgaggcga aacctcagga gtatttcaat ttgaatctgc tggtatccgg
    17581 aacgttttac gaagattaga tccagaaaac atcgaagata ttgccgcagt caatgcattg
    17641 tatcgaccag gccctatgca aaatatagat accttcattg ccaggaagaa aggaaaagaa
    17701 gccattcgct acccagatga ttcactgatc ccgattttga aaaacacata tggagtgatc
    17761 gtctatcagg aacaaatcat gcagatcgcc tcgactatgg ctggatttac attagggcag
    17821 tcagatattc tgcgacgggc agtgagtaaa aagaaaaaag acgtattaga tgaagaaaag
    17881 tcgcattttg tatcaggagc attacaacag ggatattcgg aaaaaacagc agcgaccgta
    17941 tatgaatata tcgaaaaatt tgcaaactat ggattcaatc ggtcacatgc atttgcttat
    18001 tcattcgtgg gattccaaat ggcgtatcta aaagcccatc atccaggtgc tttctttacc
    18061 agcctgatga attctgttcg gcataacaca tcaaaattga aagaatatat tgcagaagca
    18121 cgtaaaaata aactcaaact ttcctcgccg tccattaatc aaagtaattc cagttttgag
    18181 ttggtcaata aggaagaaat cagattcgga ctgacagcga taaaaggtat tcggcgtgat
    18241 tttgtagaag atatattaaa cgaaagaaaa caaaatggcc cgttccagtc agtggatcaa
    18301 ttcctcatac ggatcgataa gcgttggctg aaattagagt tactgcaatc actgacagca
    18361 gtaggagttt ttgatgaatt atctcctaac aggaaacaat tgatgcttga tctcgaagga
    18421 aaaatccaaa atatcgttta cagtggtgga agtcttgact tgctgggaat catggcatta
    18481 aaagaagaag aagtcgctga ctatacacta gaagaacgct tgaagctgga agaggagtac
    18541 ttaggtattt atttgtcagg tcatcctgca gaagggtttg atcgtcttcg tttagctaaa
    18601 caagttcagc tgattacaga tatcgtccct aatcaaccag tcaatttatt agtgatgatc
    18661 aaggaagtta gagaaattcg gacaaaaaaa ggagaactga tggcctttgt tgaaggaacc
    18721 gacagtagtg gagaaatttc tgtcacagtt tttccaactc tttatcgtaa atatcgtaat
    18781 ttgtttgccg ttgatcgagt gatctttgtt caagggcgga gcgaaatcag caaatataat
    18841 caggaactgc aactaattgg agaaagcttt gctgatccta accagttaga agcccagtat
    18901 ccttcacaaa cttgctattt gcgtattaca gaagaacggg aagatcccga gataatgaaa
    18961 caattacaag tgatttttca gaaacaccca ggatatatac cagtagttat gtatcacgaa
    19021 aaaacgcaac gaaaagtagt tctgtcagat gcttattggg tggatgacag caagagtata
    19081 aaggggcaac ttatttatct gcttggcgaa gataatgtgg ttttcaaata acttacggta
    19141 aaaaatacta cttgctttca ttttttttca tttttttatc cgtaaatcaa gacaagtgag
    19201 aaaaataatg ttagaatgtt agtcgtggtt aacgcaaaaa tggttgtaaa cctaagtcat
    19261 acttaggata aagtatttta atgaggtgaa tgtttattat gaaacgcatc ggaattttga
    19321 ccagtggcgg agacgcacct ggaatgaatg cagctgttcg tgccgttgtt cgtaaaggta
    19381 tttacgaagg actggaagta tacgggatca attatggttt tgccggttta gttgctggag
    19441 atattcgtcg tcttgatgtg gcagatgtag gtgacaagat ccaacgtggt ggtacattct
    19501 tgtattctgc acgttatcca gaatttgcaa ctgaagaagg tcaattaaaa ggaatcgaac
    19561 aattgaaaaa attcggaatc gaaggtctag tggttatcgg aggagacggc tcttatcatg
    19621 gcgcaatggc tttgacaaaa cgcggtttcc cagcagtagg tattcctgga acaatcgata
    19681 atgatattcc tgggactgat ttcacaatcg gttttgatac agcaatcaat acagtactag
    19741 aatcaattga ccgtattcgt gacacagcga cttctcatgt tcgtacattc gttatcgaag
    19801 taatgggacg caatgcagga gacatcgctt tatggtcagg tgtggctggc ggagcggatg
    19861 aaatcatcat cccagaacat gattttgata tggcttcagt tgctaaaaaa atccaagaag
    19921 gccgcgatcg cgggaagaaa cattgcttga tcatcttggc tgaaggtgta atgggcggaa
    19981 atgaattcgc tgagaaactt tcagaatttg gtgactatca tacacgtgta tctatcttgg
    20041 gacacgttgt ccgtggtgga gcaccaagcg cacgcgaccg cgtgttggca agtaaattcg
    20101 gtgcttacgc tgtcgaattg cttcaagaag gaaaaggtgg attatgtgtc ggtatccatg
    20161 acaacgaaat cgttgcgtct gacatcattg atacacttga aaacaacaaa cataaaccag
    20221 acttatcact atatgatcta aataattcac tttcatttta agagtgaaag aaaccaagct
    20281 atttaagctg tgtacagctt taaataaaca aaattgaatg ggagagtttt ttaaatgaaa
    20341 aaaaccaaaa tcgttagtac attaggacca gcaagtaact cagtagaaat tatttctcaa
    20401 ttgattgaat ctggggcaaa tgtcttccgc tttaattttt ctcatggtga ccatgaagaa
    20461 caattatcac gtatgactat ggtccgtgaa gcagttaaaa agacaggaaa agatgtcggt
    20521 attcttttag atacaaaagg agctgaaatc cgtacaactg ttcaaggcac aacagaagct
    20581 gattttggtc gtgcaggata catcaaattt gaagtaggag atcaaacacg catctctatg
    20641 gatcctgaac atgtaggttc aaaagaaaaa atcgcggtta cttacccagg attatttgac
    20701 gatgttcacg ttggcggaca cattttattt gatgatggtt tgatcgatat gcaaatcatt
    20761 gaaaaagacg aagcaaaccg tgaattagtc gtagaagtta aaaatgcagg tatgctagga
    20821 tcacgtaaag gggtaaatgc accaggcgtt tctattagtt tgccaggtat tactccaaaa
    20881 gatgctgacg atattcgttt tggtttagac aacgatatcg actttatcgc tgcaagtttt
    20941 gtacgtaaag cacaagacgt tctagatatc cgcgaaatct tagaagaaaa agacatgact
    21001 catgttcaaa tcttccctaa aatcgaatct caagaaggta tcgacaacat tgacgaaatc
    21061 atcaaagttt ctgacggtgt tatgattgcc cgcggagata tgggtgttga aatcccagct
    21121 gaactagtac caatggttca aaaacgcatt atcaaaaaat gtaatgctgc tggtatccca
    21181 gttatcactg ctacacaaat gttagaatca atgcaagaaa acccacgtcc aacacgtgcg
    21241 gaagcttctg acgttgctaa cgctgtattt gatggcacag atgcaacaat gctatctggt
    21301 gaatctgcaa acggggacta cccagttgaa gctgtttcaa caatggcacg tatcgaccaa
    21361 gaagctgaaa atgcattatc agaattaggt acattccaat tgaacgaatt cgataaaaca
    21421 gatgtaactg aaacaatcgg tttatctgta gctcgtgctg caaaaaatct aggcgtgaaa
    21481 acaatcgttg ctgcaactga atcaggatac actgcaaaaa tgatttcaaa atatcgtcca
    21541 gatgcagata tcttggctgt tacttttgac gaacgcacaa aacgtggttt gatgttaaac
    21601 tggggcgttt acccaacagt tgctgaaaaa ccatcaacaa ctgatgaaat gtttgaacta
    21661 gcagctaaaa aagctgttga attaggtttc gcatcagaag gcgatttaat cttgatcact
    21721 gctggtgtac cagttggtga acgcggaaca actaacgtga tgaaaattca aatgattggt
    21781 tctaaattat tagaagctca aggcgttgga caaaaatctg tcgttgcaaa tgcagttatt
    21841 gcttcatctg ctgaagaagc tgtaaaaaaa gcaaaagacg gaatggttct agttgtacca
    21901 tcaactgaca aagaatatat gcctgcaatc gaaaaagcag ctgctgttat cgttgaagaa
    21961 ggcggattga cttctcacgc tgcagttgta ggaattgcac aagatattcc tgtaatcgtt
    22021 ggtgctaaag gcgcaacaac aacaattaca gaaggtgaat tgattaccgt tgattcacgt
    22081 cgcggcatcg tttaccgtgg cgaaacaaca gctatctaat ttagcttaat aaaaaataaa
    22141 aacagctctt cttttacgaa gagctgtttt tttgtgctgt aacgaattca tggataactt
    22201 ggactacttg ttcttgaaga ggcgtgacga tctgctgtgt tcgttttgcc aatgaaatca
    22261 agaagcgagg ctgaacttca tctgaaagag ggaggcaaac taaatgatct tcaggatgga
    22321 cagcaatctc tgccaaaaaa ccgataccca cattttctcg gaccatactc tttaaaatcg
    22381 tcaagtcatt gctttgataa acaacttttg gatgagtatg tgtttgttta gaaagcttag
    22441 tgaatgctgc ggggtggaca taatgttcgt tcaatagtac aaactgttca tctgctatat
    22501 ctttgaaaga gacagtatcc tgcttggaaa gaggatgtgt aggtggcacg acgatcatga
    22561 aacgtttgtc caaaagtaac tctgtttcta aatattgatc cacgattggc tgtgttgagc
    22621 ctaatacagc taaatcgatc ctcccttgtc ggatcatccc gtataagtcc cgagagcctc
    22681 catcggtaat ctcaatttga ttcatcaatt catgcttgat aaagtaactt gacagtttag
    22741 gaaagtattc attgccgata ataggaggca tgccaaaacg aagctttttc ttttgcaact
    22801 gagtgatttc agctcttgca gtagaaatct cttggagaat ggtttgtgca tggagaaaca
    22861 gctgctttcc agaaggcgtg acgattaatt cgtgatgcga tcgatctctt gtaataagta
    22921 cggtgtcaaa ctctttttca agacgttgta atgcatatgt aatagtaggt tggctcacac
    22981 ggaaataatt agccactttc gtaaaatttt tttgtttgac gatttggaca tagtattcga
    23041 tatctttcgt gttcatgaag atcatccgct cctttaatgg aataatgatt attgtaaatg
    23101 attctgagga tgatagcaaa cagaggcttt ctaataaaga tttttctgtt tttttatgaa
    23161 ctgcaaggtg tcaagaaaat ttcattgaca acatgctttt tgccctgtat aattgctttt
    23221 gttgcttagg agtgatgaaa aatgaaatgg tctttattgg aattgagaaa ataccaggag
    23281 acaccgttaa cttttcatga gacgctcgac ttgaaagccg ctctcatgaa gcgagacaat
    23341 ttaatacttg atgttgcgcc agtggaagtc gaaggtttgg tctcagttga taagaaaggc
    23401 tatatcgttc attacactgt gcagacgact ttgacagttc cttcaacacg ttcactagaa
    23461 cctgttgaac tgcccatgga tttttctgta gacgaagtat tcatgacaga agaacaattc
    23521 caaagtagag atgaacaaat gtctgcagat gagattttgc taattgaagg tccattattg
    23581 aatttagcag agtcgattga agacaacatc ttattggcga ttccaatgcg ggtattgaca
    23641 aaagaagagg aagcagcaac agaacttcca aaaggcaatg attgggaagt tgtttccgaa
    23701 gaagaatacc agcgcagaaa aatggaagaa gccgagaata aaattgatcc tcgtcttgca
    23761 aaactatctg aactttttga ttctaaatca aaagacgacg ataacaagta agattggaat
    23821 atattgtgtc taatttagat acaagacttt acaaggaggt gtagcaaaat ggcagtacca
    23881 gctagaagaa catcaaaagc taaaaaagca aaacgtcgta cacactacaa attgacaatc
    23941 aaagggttaa acgaatgtcc aaactgtggt gaaatgaaaa aaagccatca cgtatgtgca
    24001 aattgtggtt actatgatgg taaggacgta atgtcaaaag aagcttaata cattttaatt
    24061 agaaaatgta aaacctcaag gctaaccatg gagccttgag gtttattttt ttgtagtcgg
    24121 taaataaaaa at
//
LOCUS       DACEDM010000047        23828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEDM010000047 DACEDM010000000
VERSION     DACEDM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.047"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IWO50_001721"
     CDS             complement(53..787)
                     /locus_tag="IWO50_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0358845.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IWO50_001722"
     CDS             1114..1524
                     /locus_tag="IWO50_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0358846.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IWO50_001723"
     CDS             1517..2218
                     /locus_tag="IWO50_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0358847.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IWO50_001724"
     CDS             2218..3804
                     /locus_tag="IWO50_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0358848.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IWO50_001725"
     CDS             3823..4044
                     /locus_tag="IWO50_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0358849.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IWO50_001726"
     CDS             4041..4802
                     /locus_tag="IWO50_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0358850.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IWO50_001727"
     CDS             5182..5691
                     /locus_tag="IWO50_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0358851.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IWO50_001728"
     CDS             complement(5766..6305)
                     /locus_tag="IWO50_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0358852.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWO50_001729"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWO50_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0358853.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IWO50_001730"
     CDS             7372..8169
                     /locus_tag="IWO50_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0358854.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IWO50_001731"
     CDS             8197..8832
                     /locus_tag="IWO50_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0358855.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IWO50_001732"
     CDS             8852..9625
                     /locus_tag="IWO50_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0358856.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IWO50_001733"
     CDS             10033..10830
                     /locus_tag="IWO50_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0358857.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IWO50_001734"
     CDS             10808..11221
                     /locus_tag="IWO50_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358858.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IWO50_001735"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IWO50_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0358859.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IWO50_001736"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IWO50_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0358860.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IWO50_001737"
     CDS             15998..16558
                     /locus_tag="IWO50_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0358861.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWO50_001738"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWO50_001738"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0358862.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IWO50_001739"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IWO50_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0358863.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IWO50_001740"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IWO50_001740"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0358864.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IWO50_001741"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IWO50_001741"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0358865.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IWO50_001742"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IWO50_001742"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0358866.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IWO50_001743"
     CDS             21905..22483
                     /locus_tag="IWO50_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0358867.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IWO50_001744"
     CDS             22535..23017
                     /locus_tag="IWO50_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0358868.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IWO50_001745"
     CDS             23134..23508
                     /locus_tag="IWO50_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358869.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5116 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DACEDM010000048        23698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEDM010000048 DACEDM010000000
VERSION     DACEDM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.048"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO50_001746"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO50_001746"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0358870.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWO50_001747"
     CDS             complement(1172..1867)
                     /locus_tag="IWO50_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0358871.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWO50_001748"
     CDS             complement(1880..2596)
                     /locus_tag="IWO50_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0358872.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWO50_001749"
     CDS             2926..3534
                     /locus_tag="IWO50_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0358873.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWO50_001750"
     CDS             3859..4182
                     /locus_tag="IWO50_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0358874.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWO50_001751"
     CDS             4364..5734
                     /locus_tag="IWO50_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0358875.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWO50_001752"
     CDS             complement(5864..7165)
                     /locus_tag="IWO50_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0358876.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWO50_001753"
     CDS             complement(7519..9510)
                     /locus_tag="IWO50_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0358877.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWO50_001754"
     CDS             complement(9654..9920)
                     /locus_tag="IWO50_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0358878.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWO50_001755"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWO50_001755"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0358879.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWO50_001756"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWO50_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0358880.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWO50_001757"
     CDS             13264..13827
                     /locus_tag="IWO50_001757"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0358881.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWO50_001758"
     CDS             complement(14080..14601)
                     /locus_tag="IWO50_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0358882.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWO50_001759"
     CDS             complement(14604..15446)
                     /locus_tag="IWO50_001759"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0358883.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWO50_001760"
     CDS             complement(15439..16164)
                     /locus_tag="IWO50_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0358884.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWO50_001761"
     CDS             16340..16636
                     /locus_tag="IWO50_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358885.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWO50_001762"
     CDS             17204..18796
                     /locus_tag="IWO50_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0358886.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWO50_001763"
     CDS             18809..19549
                     /locus_tag="IWO50_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358887.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWO50_001764"
     CDS             19717..21066
                     /locus_tag="IWO50_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0358888.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWO50_001765"
     CDS             complement(21194..21784)
                     /locus_tag="IWO50_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0358889.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21931..23283
                     /gene="brnQ"
                     /locus_tag="IWO50_001766"
     CDS             21931..23283
                     /gene="brnQ"
                     /locus_tag="IWO50_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0358890.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6793 a         4772 c         4511 g         7622 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caagaactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat
    21961 ctttttatag gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc
    22021 gttcatttgg gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt
    22081 accggtatcg gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt
    22141 gtatatgaat tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt
    22201 tacttagtta tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt
    22261 ggtttagcac catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt
    22321 gtattttttg tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt
    22381 ggaaagttcc tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt
    22441 ttgaatccac taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct
    22501 tttttcacag ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt
    22561 gggatcatca ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct
    22621 aaagacacga tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg
    22681 ctcgcttatt taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc
    22741 acgctggctc agattgctga tcattattta ggtacttatg gcagtatctt attagcattt
    22801 atcgttatat tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca
    22861 ttcacagatt tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt
    22921 ccttgtttag cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg
    22981 atgttcatct atccattagc aatgacactg attttactag tgttagttgg tcctttgttt
    23041 aagcagcgta cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt
    23101 gatgggttga acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta
    23161 tatgttgcgg aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca
    23221 gttatcggtt ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat
    23281 tgataaaaga ggttgtgaaa gagacgttca gccttgagca ataaaaagta attttgaaaa
    23341 atggcttctc acatttttgc ataaatttaa cttaataccg agaaactgac ttttgaacac
    23401 cgtttattga gaggtcgtga aaaagccgct tagtttcaag tattaagaaa tattttgtga
    23461 aaatggcttc tcacattttt gcataaattt gacttaatac cgagaaactg gcttttgaac
    23521 accgtttatt gagcgtctat aaactgacct gagtattaga gaaacaaaac ggcaaagtag
    23581 ctccatatac tttgccgttt tgttttgaac taataccgaa gagaagctga tcaaacaaca
    23641 tttcttttat aatatagatt ttagacatat atatgatata aaaatagttt tcgcaaaa
//
LOCUS       DACEDM010000049        22984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEDM010000049 DACEDM010000000
VERSION     DACEDM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.049"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..560)
                     /locus_tag="IWO50_001767"
     CDS             complement(105..560)
                     /locus_tag="IWO50_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358891.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="IWO50_001768"
     CDS             complement(714..1922)
                     /locus_tag="IWO50_001768"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0358892.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="IWO50_001769"
     CDS             complement(2034..2366)
                     /locus_tag="IWO50_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0358893.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="IWO50_001770"
     CDS             2514..3389
                     /locus_tag="IWO50_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0358894.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="IWO50_001771"
     CDS             complement(3471..4052)
                     /locus_tag="IWO50_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0358895.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="IWO50_001772"
     CDS             complement(4058..5317)
                     /locus_tag="IWO50_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0358896.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="IWO50_001773"
     CDS             complement(5319..6365)
                     /locus_tag="IWO50_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358897.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6600..6875)
                     /locus_tag="IWO50_001774"
     CDS             complement(6600..6875)
                     /locus_tag="IWO50_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0358898.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7118..8428)
                     /locus_tag="IWO50_001775"
     CDS             complement(7118..8428)
                     /locus_tag="IWO50_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0358899.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="IWO50_001776"
     CDS             complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="IWO50_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0358900.1"
                     /translation="MTEFDQNQENNQSMEDGMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9974..10654)
                     /locus_tag="IWO50_001777"
     CDS             complement(9974..10654)
                     /locus_tag="IWO50_001777"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0358901.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10703..11338)
                     /locus_tag="IWO50_001778"
     CDS             complement(10703..11338)
                     /locus_tag="IWO50_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0358902.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11404..12807)
                     /locus_tag="IWO50_001779"
     CDS             complement(11404..12807)
                     /locus_tag="IWO50_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0358903.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12794..13825)
                     /locus_tag="IWO50_001780"
     CDS             complement(12794..13825)
                     /locus_tag="IWO50_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358904.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13856..14077
                     /locus_tag="IWO50_001781"
     CDS             13856..14077
                     /locus_tag="IWO50_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0358905.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14242..14979)
                     /locus_tag="IWO50_001782"
     CDS             complement(14242..14979)
                     /locus_tag="IWO50_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0358906.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15132..16472)
                     /locus_tag="IWO50_001783"
     CDS             complement(15132..16472)
                     /locus_tag="IWO50_001783"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0358907.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16709..18232)
                     /locus_tag="IWO50_001784"
     CDS             complement(16709..18232)
                     /locus_tag="IWO50_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0358908.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18610..19221)
                     /locus_tag="IWO50_001785"
     CDS             complement(18610..19221)
                     /locus_tag="IWO50_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0358909.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19344..19469)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19551..20267)
                     /locus_tag="IWO50_001786"
     CDS             complement(19551..20267)
                     /locus_tag="IWO50_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0358910.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20273..20842)
                     /gene="scpB"
                     /locus_tag="IWO50_001787"
     CDS             complement(20273..20842)
                     /gene="scpB"
                     /locus_tag="IWO50_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0358911.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20826..21623)
                     /locus_tag="IWO50_001788"
     CDS             complement(20826..21623)
                     /locus_tag="IWO50_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0358912.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21593..21964)
                     /locus_tag="IWO50_001789"
     CDS             complement(21593..21964)
                     /locus_tag="IWO50_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0358913.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21983..22870)
                     /gene="xerD"
                     /locus_tag="IWO50_001790"
     CDS             complement(21983..22870)
                     /gene="xerD"
                     /locus_tag="IWO50_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0358914.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6443 a         4914 c         3898 g         7729 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     6481 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     6541 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     6601 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     6661 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     6721 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     6781 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     6841 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     6901 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     6961 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     7021 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     7081 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     7141 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     7201 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     7261 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     7321 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     7381 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     7441 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     7501 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     7561 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     7621 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     7681 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     7741 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     7801 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     7861 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     7921 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     7981 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     8041 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     8101 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     8161 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     8221 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     8281 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     8341 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     8401 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     8461 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     8521 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     8581 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     8641 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     8701 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     8761 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     8821 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     8881 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     8941 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     9001 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     9061 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     9121 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     9181 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     9241 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     9301 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     9361 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     9421 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     9481 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     9541 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     9601 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     9661 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     9721 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     9781 cttttcattc cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     9841 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     9901 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     9961 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    10021 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    10081 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    10141 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    10201 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    10261 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    10321 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    10381 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    10441 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    10501 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    10561 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    10621 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    10681 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    10741 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    10801 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    10861 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    10921 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    10981 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    11041 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    11101 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    11161 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    11221 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    11281 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    11341 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    11401 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    11461 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    11521 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    11581 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    11641 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    11701 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    11761 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    11821 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    11881 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    11941 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    12001 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    12061 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    12121 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    12181 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    12241 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    12301 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    12361 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    12421 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    12481 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    12541 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    12601 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    12661 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    12721 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    12781 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    12841 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    12901 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    12961 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    13021 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    13081 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    13141 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    13201 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    13261 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    13321 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
    13381 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    13441 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    13501 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    13561 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    13621 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    13681 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    13741 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    13801 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    13861 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    13921 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    13981 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    14041 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    14101 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    14161 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    14221 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    14281 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    14341 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    14401 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    14461 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    14521 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    14581 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    14641 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    14701 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    14761 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    14821 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    14881 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    14941 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    15001 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    15061 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    15121 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    15181 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    15241 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    15301 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    15361 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    15421 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    15481 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    15541 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    15601 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    15661 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    15721 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    15781 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    15841 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    15901 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    15961 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    16021 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    16081 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    16141 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    16201 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    16261 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    16321 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    16381 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    16441 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    16501 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    16561 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    16621 agctattgac ttgtccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    16681 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    16741 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    16801 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    16861 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    16921 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    16981 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    17041 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    17101 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    17161 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    17221 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    17281 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    17341 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    17401 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    17461 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    17521 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    17581 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    17641 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    17701 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    17761 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    17821 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    17881 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    17941 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    18001 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    18061 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    18121 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    18181 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    18241 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    18301 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    18361 cctattccct cctctttttt cagtttttta aagctttctt ctttttcatt gtagcgtttc
    18421 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    18481 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    18541 tacattattg catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct
    18601 ttttgatatt tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa
    18661 ccatggaaga agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat
    18721 caagttgaaa ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg
    18781 tgttgcgttc agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat
    18841 actcataaaa atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc
    18901 tttatgctgt ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga
    18961 taagaaacta gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg
    19021 taatactgaa cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct
    19081 gatcaatacc gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg
    19141 aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt
    19201 ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt
    19261 ttgacgtttc tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat
    19321 ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg
    19381 gaatttcacc gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg
    19441 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    19501 acatatctat tcaaaaaacg caacatctaa caattataaa aagtaagtgt tcattttgag
    19561 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg
    19621 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    19681 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    19741 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    19801 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    19861 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    19921 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    19981 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    20041 gcaacagcag aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga
    20101 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    20161 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    20221 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc
    20281 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca
    20341 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    20401 cagttgttcc atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca
    20461 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    20521 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt
    20581 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    20641 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    20701 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    20761 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    20821 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    20881 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    20941 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    21001 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    21061 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    21121 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    21181 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    21241 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    21301 aattcttctc gaggatcttc ttcccataat tcttcatcgt ctgtgccgaa ttcttggaca
    21361 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    21421 gcaacagcta gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    21481 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    21541 tcgagaggac cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    21601 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    21661 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    21721 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    21781 aaataccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    21841 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    21901 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    21961 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    22021 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    22081 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    22141 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat
    22201 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    22261 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    22321 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    22381 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    22441 cagtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    22501 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    22561 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    22621 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    22681 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    22741 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    22801 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    22861 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    22921 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    22981 tctt
//
LOCUS       DACEDM010000050        22835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEDM010000050 DACEDM010000000
VERSION     DACEDM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.050"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..464
                     /locus_tag="IWO50_001791"
     CDS             51..464
                     /locus_tag="IWO50_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0358915.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(922..1197)
                     /locus_tag="IWO50_001792"
     CDS             complement(922..1197)
                     /locus_tag="IWO50_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358916.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1651..1857
                     /locus_tag="IWO50_001793"
     CDS             1651..1857
                     /locus_tag="IWO50_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358917.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            2053..2313
                     /locus_tag="IWO50_001794"
     CDS             2053..2313
                     /locus_tag="IWO50_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358918.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            2918..3751
                     /locus_tag="IWO50_001795"
     CDS             2918..3751
                     /locus_tag="IWO50_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAQ0358919.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            3744..5159
                     /locus_tag="IWO50_001796"
     CDS             3744..5159
                     /locus_tag="IWO50_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAQ0358920.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            5171..6700
                     /locus_tag="IWO50_001797"
     CDS             5171..6700
                     /locus_tag="IWO50_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0358921.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            6792..7670
                     /locus_tag="IWO50_001798"
     CDS             6792..7670
                     /locus_tag="IWO50_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0358922.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            7672..7989
                     /locus_tag="IWO50_001799"
     CDS             7672..7989
                     /locus_tag="IWO50_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358923.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            8038..8739
                     /locus_tag="IWO50_001800"
     CDS             8038..8739
                     /locus_tag="IWO50_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0358924.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            8754..9644
                     /locus_tag="IWO50_001801"
     CDS             8754..9644
                     /locus_tag="IWO50_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ0358925.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            9667..9882
                     /locus_tag="IWO50_001802"
     CDS             9667..9882
                     /locus_tag="IWO50_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358926.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            9894..10235
                     /locus_tag="IWO50_001803"
     CDS             9894..10235
                     /locus_tag="IWO50_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358927.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            10235..10606
                     /locus_tag="IWO50_001804"
     CDS             10235..10606
                     /locus_tag="IWO50_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358928.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            10599..10994
                     /locus_tag="IWO50_001805"
     CDS             10599..10994
                     /locus_tag="IWO50_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0358929.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            10996..11373
                     /locus_tag="IWO50_001806"
     CDS             10996..11373
                     /locus_tag="IWO50_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358930.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            11374..11982
                     /locus_tag="IWO50_001807"
     CDS             11374..11982
                     /locus_tag="IWO50_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0358931.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            11982..12323
                     /locus_tag="IWO50_001808"
     CDS             11982..12323
                     /locus_tag="IWO50_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358932.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            12565..15354
                     /locus_tag="IWO50_001809"
     CDS             12565..15354
                     /locus_tag="IWO50_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ0358933.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            15344..16084
                     /locus_tag="IWO50_001810"
     CDS             15344..16084
                     /locus_tag="IWO50_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0358934.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            16081..18843
                     /locus_tag="IWO50_001811"
     CDS             16081..18843
                     /locus_tag="IWO50_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0358935.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            18856..19764
                     /locus_tag="IWO50_001812"
     CDS             18856..19764
                     /locus_tag="IWO50_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0358936.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            19764..20384
                     /locus_tag="IWO50_001813"
     CDS             19764..20384
                     /locus_tag="IWO50_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358937.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            20388..20837
                     /locus_tag="IWO50_001814"
     CDS             20388..20837
                     /locus_tag="IWO50_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358938.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            20837..21331
                     /locus_tag="IWO50_001815"
     CDS             20837..21331
                     /locus_tag="IWO50_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358939.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            21345..21692
                     /locus_tag="IWO50_001816"
     CDS             21345..21692
                     /locus_tag="IWO50_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358940.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            21685..21825
                     /locus_tag="IWO50_001817"
     CDS             21685..21825
                     /locus_tag="IWO50_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0358941.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            21925..22314
                     /locus_tag="IWO50_001818"
     CDS             21925..22314
                     /locus_tag="IWO50_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0358942.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            22311..>22835
                     /locus_tag="IWO50_001819"
     CDS             22311..>22835
                     /locus_tag="IWO50_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0358943.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLS"
BASE COUNT         8206 a         3191 c         4731 g         6707 t
ORIGIN      
        1 caataaatcc aacacattta ttatatcaca taaaggagcg gtttgacttg atgcaattgt
       61 tacgagaggt agatttcaaa cagacaagat gtaatgcgag agatgtgctg aagaactttc
      121 ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgccg attataacgg
      181 atatgccgaa ggcaccgaag catggcaata aggcagaaga cgcgattatt cagatgatgg
      241 atatagaaac ggagagagat gcgattctag cggctttgat ggctcttagt ctgattagtc
      301 gtcagatact ctactacagc ttctgtgtgc cagatagctt ctcaaactac agaattagcc
      361 gtgaagtggg ttattcagaa agaagtatac aacggatgaa gtcggaagct ctaatagagt
      421 ttgcagaagc atataaacac ggaaaaataa ttgcttataa ataatttggc ggttttttgg
      481 cggaatgatg gcggttttta gacatttacc agtgttatta tggtagtgtc gaaagattag
      541 gaaacaggat cgacaaaata aacgaaggga ggcaatctcc ctcatcgttt taaacttctt
      601 cttagataga cagcaactaa ttatgaagaa aaggatgtga attcaactcc tcctaaattc
      661 ttcatcggtt gctgtttatt ttgattagac agcagcactc acaaaactaa acatgttctt
      721 gtcgtctttc aactgctgct gtctattaat ttatgtattg gagggaagaa aaatgaaatt
      781 atcagtagaa ggcactccag aagaaataca agaattgctc caagctattg gtagtagcaa
      841 ggagcaaaag gtaagtgttt ctaattcaga tattgacggt atttgcaatt gtttaaacag
      901 agaaaaaata tctaatcctt actaagagaa aatatgttcg ttaatgaatt tccaattttc
      961 ctctgtagct ctaccttgat aatttttttg cagttcacag atgaataagg aatcgttatt
     1021 atcaagtact gtatttaatt tttcacacat ttgatttgga ttcaaattag ttttaactaa
     1081 ccaaaaagat ttttgtagct ttatatatgc cccaaattct tttattattt caaatacttt
     1141 atcatacttt tgccctgggt tattaagatc ataactaatc atgtagggtt tatacatgtt
     1201 gtcttgcaca tccttgtttt atatttcagc ggaccactcg ctgataaata aaattatacg
     1261 cttagtattt attttcacaa tattaattta tcgtaataac ttttgagtaa atagaacaaa
     1321 aaacctgcac tagtttccgc tagtacaggc agtgactata tcggttgata atctagcata
     1381 tattaaaaat aattgcaaga aaagaaattt atagtggttt ataccaaatt atcaaagcaa
     1441 tgttattttg ttgctgtcta ttgtttttta attattcaca cgataactaa aggtgggtga
     1501 agagaaatga ttccattaat aatttcaatt tttgcgctct gtcttaatgt ctatatgatt
     1561 ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa gaataatttt atagtgtcac
     1621 tgtggcggaa agggtagacg ctaagcatgt gtgctaggtc aatgcttcgg caaccatgca
     1681 aggttcgatt ccttgccagc gacattaaat gcctatgacg gttacgacta ccgaaaaaag
     1741 atcgttaaga agctatacgg tgctacgtac ggcaatgtag taagtgtgct atctgtacac
     1801 caccaagctt cggtcactgt ggcggaagta gaagacgcag cggtaaatgg cgagtagcct
     1861 cgtgagagcc tggtaagttc tcgtgagtgg tgcaatccca ctccagcgac tttaagcaac
     1921 cgaggcatcg gcggtttaaa aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg
     1981 tgcaagttac tattacatat tagatcactc gttgagtggt ctttttattt tagaaaagag
     2041 aggattttga aaatgaatca tgagaagttt atcgaaaaat gcaaggctat tgtacgtgaa
     2101 agaattgaga atgagattgc tgacctaagt ggagcagtac ctgagtttag tgttttcata
     2161 gtctggtcat gcaaaacact acagaatagc aaagcattag tcagcgctag cttaaaagga
     2221 gcaccgtatt ttgaaattac gttgaacggg gacaaaggtg aaatctatgt agatacttat
     2281 ctcaaaaaat caaatgaatg tatcaaagtc tagcaggtgc taggcttttt ctttacataa
     2341 aggaggaatg atctcatgaa acaatatacc gctaaagatt tcgaggaaat gaagcgatta
     2401 aagaaggact atgaagaagt tgatatggag ctaactgttg gagtcattca acgaagactg
     2461 cgggtcggat tagagacagc aaaggctatt tacaatgatc taaatgctat tgaagagaag
     2521 aatggctaat gaggaactac tggtatatat cgctaactaa tgaatatcct cgaaccattg
     2581 atgattgttc agtgcgtgtt gtgcgttctg tacaaatcaa agggaagtac tctattgtcg
     2641 aaatggggag agaagcagaa ccatgtgaga ttgatgcgtg caagctcgtt tattgcggtc
     2701 atggtttcta tgatgaacca aacattcaaa ataatattaa caagaatttg agggattaga
     2761 atgcaagaaa tggcttaaaa atctccaatc aggacaaatc aagttagaca aagtttccga
     2821 tttaaagata ttaattgaag cagatctaat gttgaaagat attgaaaatt agaaaacaaa
     2881 actcaaccta agaagattgc gaggtggtgt gtattgaatg gcaagacaac gtgatccaag
     2941 acgtgatgaa gccaaaagaa tttggttaga atccaacgga gaaaagcagt taaaggaaat
     3001 tgcatctgaa ttaaatgttt cagattctca ggttagaaaa tggaaatcgc aagacaaatg
     3061 gagcgctgaa ttgaaaagta acgttaccaa tggcaaaagt aacgttacta atcaaggtgg
     3121 cgctcctatt ggtaatcaaa atgctaaagg taacaaagga aatagccgag cttctccgcc
     3181 agtgggtaat aaaaacgctt tgaaaacagg cgagtatgaa accatatttt ttgagacact
     3241 aagtgatgaa gagaaggaca tctattctag tctgaatgat aatccttctt ttgttttgtc
     3301 tgaagaaata cgtctactta agataagaca atttcgtatg atgaagagaa tccaacaagc
     3361 tgaagctgga ctaaatgatg aagaagtcga acgattgcag cagctaagaa agattaaaaa
     3421 tccgattgaa aaaaatggta aaaagctaga aatcaagcgt gaggttatgc aagatgtgca
     3481 gattagcaga aaaaaacatc gcaaaattga tgatattctt tcaattgaag attcattgac
     3541 tcggattagc aaccagttag ctaaagccat caagcaaatg aatgaacttt atatgaatga
     3601 atacagaact gatttaatta aagctcagac tgataagatc caagctgaga caaatgaaat
     3661 tggcggaaat aattcaggtg aagaaataga agaatggaaa caggcagttt taaatgccgc
     3721 aaacaaacgg gcggtgaaag aaaatgaata atgaatttat tccttttgct gatattggtt
     3781 ctgccattga ttattactat gataaaccag tagctttttg ccaagatatt ttgcatttga
     3841 atcctgatga atggcaagaa aatgttttaa atgatttagc tgaattttca aaggtttctg
     3901 ttcgttctgg tcaaggagtt ggaaaaacag cattagaagc aggagcaata ctttggttct
     3961 taacgtgtcg accctacgct aaagtaatag caacagctcc gacaatgaag caactttacg
     4021 atgtactttg ggcagaggta gctaaatggt taaatgatag cttgatcaaa aacttactga
     4081 agtggacaaa gaccaaaatt tatatggttg gtgattcaga gcgttggttt gctacggcta
     4141 gaacagcgac taaaccagaa aatatgcaag gttttcacga ggaccatatg ttgattgtgg
     4201 tagatgaagc ttctggtgtg tctgatccaa ttatggaagc tattcttggt acgctatcag
     4261 gttttgataa taagctgttg atgtgtggaa accccaataa tattgaaggt gttttttacg
     4321 attcccacaa ttcagaccgt gataaataca gagttcataa agtatcaagc tatgatagta
     4381 aacgtacaaa caaagacaat atagaaatga ttcttaaaaa atatggaaaa gaaagtgatg
     4441 ttgctcgtgt ccgtattttt ggagaatttc ccaaaggtgc gttggattca tttatcagtc
     4501 ttgaaacggt tgaattggct acagaaaaac aaattagtga ttctttagtc aataaaacaa
     4561 cggttgctca tattggtgtt gacgtagctc gatatggtga tgattctacg attctctttc
     4621 ctagaattgc taccagggca ttggagtatg agaagtattc aaaacgtagc accatggaaa
     4681 caacaggata tgtcatcaac atggccaaga atctaatgag tcaatatccg agtattgata
     4741 aagtgatgat taaagtcgat gacactggtg tcggaggtgg tgtaaccgac cgcctagaag
     4801 aacttataga agacaaacat tatccttttg aggtgtttgg agtgaataac ggttcaacat
     4861 cagaagacga tttttacgat aatttaggta ctcaactatg gggaaacatc aaggaaatgt
     4921 tagaagaaaa tatgacagca aatcttaacg gagaacagcc tgttattgaa ttgccttctg
     4981 atagttcgtt aatcaaagaa ttaagtactc gcaaattcaa aatgacaagt agaagtcgta
     5041 tacgtttaga aagtaaagat gatatgaaaa agcgaaatat tggtagtccc gatattgctg
     5101 acgcactggc tttagcgttt tatgagccac caagtcacta tcaatttatt caattttagg
     5161 aggtgagctt ttgattagta cagtattatc tttagagaga tataagaagc tacgtgttaa
     5221 atatgcaaca caaattgaag atggaatgtt tgatccgaat ggttttatag aagatatgaa
     5281 accatttttt gctgatcgcg aaagaaaata tctagcttat actagcgaaa agaatgaaat
     5341 agataataga ccaaagccta acactgatat tgtaaaagtt aataataaac ttcatgctgg
     5401 tatgtattca attgtcgtag accaagcagt caatcatttt actggtatac ctatcaaatg
     5461 ggattacgat gtatcggaac aaaaaagaac actcattcaa agattaaaag ataaattctt
     5521 aaaaaatgat atagaacttc cgacagttcc agaagctttt aacaaattaa caagcaatct
     5581 tgattccatg agattcgcaa tgcttgattc tgaaactgca acttttcaag gagcttgtgg
     5641 agtagctttc cgattgttag agcctgtgga agaagatgat ggttggaagt taagagcaag
     5701 taatattgaa ccttggagag cagaaagata tggaaatgcc ggaatctata tcaaagaaaa
     5761 gtatgactct taccagaaaa aattttttca agaaatgaaa gttatgacaa gaaataaaat
     5821 tctgacatat gcttgctatg gtgatttgaa ttttgttaca agcggaacgt tcaaaaaaat
     5881 tgatgagacc gataaccctt tggggacgat taccttggca gaatttaaaa ataatacgaa
     5941 tcgttattgt gattttgaag tagctgagga aattggagat gcaattgatc gtgctttgtc
     6001 tgatcaacaa aacgaaatcg aacaatttaa gcttgcgtac atgcttgtta ctggcacaac
     6061 aatgagcaaa ggcactgcta aagaaatgat gaatcagcta gggattatca acttaaaaga
     6121 ccctactgca aaagctgaat atgtcacaaa aaatttagca aaagatttca atgaatatca
     6181 tatggatcta ttgaaaaaac agttttacac catttgtaaa gcaatcgatt ttaacgatga
     6241 ggtgtttaaa tcaaacagtt ctggagaagc tcgcaagtgg caaatcatta gcctagaagc
     6301 gaaaacaaat actaaagagc aatattttag agaaggattg aaagaatgcg cagaaacaat
     6361 tgctgctttt cttaagtttc atgacaaagt agaaattgag cctgaaaaaa ttatttttac
     6421 tttctctaga tctttgccaa ctgatttaag ttatttagct gaagctttac ctaaacttgc
     6481 accatacgta tccaaacgaa ctattcagag ccaaattcca tttgtaacag atgttgatta
     6541 tgaaaatgaa atgatggaat tggaaagtgg aagtgcttat ccagatagtg aatacaattt
     6601 tggcggaggt ggcaatagtg acgatagaaa cgaagtattg gaccaaacgt cgagaactgg
     6661 aggatcaagc aaggctcaaa caagaaaatc agacacttaa aaaattaact agtgtatttc
     6721 ctgaagcact gaaagagata caagcaaaac tattatcaca agctgactta cacaatatca
     6781 cttatccaga aatgatggag ttttatagta caagtaatca gaaaaaatat cgtgaatatg
     6841 tggaaaaaaa ttataagtca ttaaaaatgt atgatgcaaa atacaaagag tttatcgatg
     6901 aattttttcc accatttgac tatgcaaaag tcaatcgctt attacaaata cgatcagatg
     6961 tatttaaaat tcttgcagaa tatgcgatgg atgcagatgt gaatcaatat ttttctgatc
     7021 gcttagagga aattcttcaa agaacatatt cttctaatgc taatgttttt gttcaacttt
     7081 taaacgttga tataccaaat tatttaccag aaaatgaaat taaacattat ttgaactatc
     7141 catggtcagg aaagacattt tcaagaagac tttggggaaa tatttcatcg cttgaacaga
     7201 aactctctaa tgctattgta aaaagtgttg ctagtggaga aggcgttata cacgcattaa
     7261 acaccatgag attagactca gaaatttgtg acatgtttaa gttagaagaa tcaaagtata
     7321 acaaagcgat agaaaatctc gttcgaacgg agtatgcaaa atttgcacaa gatggtattg
     7381 aaaaatcata tttagaaaca ggtattgagg aatacaacgt attgactgca aaagatgaga
     7441 gagtttgccg gatttgtgga ggaaaggcaa gtaagaatcc ctataaactg aaagatgctg
     7501 tcataggtga aaatcgagca cctttccata gtcgttgtag atgtacggat gttcctaatt
     7561 taccaaaatt aggaaaggat attgatgaag aatatgaccg tttatttggc gatttattag
     7621 atgagtttgc acatgattct tttggaatta atttgaaacg gaggaagtag aatgaaagat
     7681 ttttttgaag cagtactaac aattaatgta aatgctgata ttgcagaagc ctacaaaaca
     7741 gctattgagt ctgagaacca tcctaatggc ttgagagacc attggaatgg caattatgcc
     7801 tacgtggtta ttggcgatca aactgttaat tatcaagata atactccagt tgataagaat
     7861 accgttaatt taacgattca attattatct cattcattac caaatttaaa agaaacagtt
     7921 gattggtatg aaaagatggg atgtattgtt gttagaactg actacaaaga aggaaagtct
     7981 agtaattagg cttttttatt ttgtccgaaa tgacgttaaa ctagcgcaat actgggcttg
     8041 gttgaatggt ggggcgcaac tattaaaact caaagcaatg cggggcgtga aaacgaatcg
     8101 tggggcgaaa ggagaatgag catgaaacac aaatcattaa tgccaatgaa tttgcaatac
     8161 tttgccgaag gtgacgatca aaagttttct tttgatgact tcaaatcttt tgtggaatcc
     8221 aatgaagaag ctcaaaaatt tattcagtca caatctcaaa gtgttgccga caaacaattg
     8281 gaagcttgga aacaaaataa cttagataaa attaaacagg ataccatcaa ggaatatgag
     8341 gaatctaaga aaaacaagtc acctgagcaa attcaactgg aaaaactaca agctgaattt
     8401 gaagcagaaa aagcgttgcg tgtgacaagt gataataaag catttgttgc agaacaaatt
     8461 gctggattag aactagatgg agagttaaaa gagtctattt ctcaatttat gctaaataat
     8521 cttgttagtt cggatacaga tttcactaag aatgctgttg aaggttttac aagtgtcttg
     8581 aatgcaatta atgagaaaca tgcagatgca ttaaaagaac tacaaatgaa gtctgcattt
     8641 ggtggaactc aacaatcgaa taaccaggtt cagcagaaca atgaaacatt aacaaatcca
     8701 gaagaacaat taggacaaat tcttcaacaa tttaactagg agagtgaaaa attatgaaaa
     8761 aaacatcttt aaataattta gagtatttgg atatttcacc agcgattaat gctatgcaag
     8821 taccaaatac acctttttta agctatttat ttggtgctgg aaaaacagag ccagcaaact
     8881 cgacagaaat taaatggcgt gaatatgata tcaacaacga tgattcttct gaaaaacttg
     8941 agggcggaga atatccagat gctgaatcag gtcgaacttg gtttaacaac tatactgaaa
     9001 tttttagaaa atcaacctct gtatctggta cattagatgc tattaatgtg aatggagtcg
     9061 gaaatgaatt aactaatcaa gtagcactac gtggtatgga aatgaaaatt gatttgaacc
     9121 gaaaattgat tactggtgta aaagctgatg aaaatggttc taaaggtcgt cgaatgaatg
     9181 ggattttgaa cttgatcaat tcagcaaata aggcagaaac agccactgcg ggtgcagtaa
     9241 caagaaaaga tatcgatact ttatttaaat tgatgtatga aaaaggttat atgggagaaa
     9301 aactatgctt gatttctcca gatatgcagg agttaatgac tgatgagtta gatggaaaat
     9361 caacaaaaat tgttcagttc ggggaaagag taacttttgg attgcaaatt ggaaatatcg
     9421 tgtctaatta cggtacaggt attgctctac tagaaccatc attgccaaaa ggaacaattg
     9481 ccgcaatcga tactaattat gtgaaactac gtccattacg tgaatggaga gcagaagaac
     9541 ttgcaaaaac aacggattct agacgtatcg gtcttgtagg tgaatactct cttgaataca
     9601 atgcttcaaa ctctggggca attttaaatt taaagtctga ataaaaggga gttagtactc
     9661 ccttttttga taggaggaat tacagtggta aaaaaagatg aaactaaaaa agatgaagtc
     9721 gtgaaatata gagtaggtaa aactaaaaat tttgttgggt ttgttcatcc taaaactcgt
     9781 agatttatca cagcagattc aaataacgaa tttatcattt ctatagatga taaagaagca
     9841 attgcaattt tggaagatgc aattgatgtt aatgaaattt aggaagtgat ctgatggatg
     9901 aatcgctaaa aacggaaatc attgagtcta caaaagaaga ttttccagat ttgagtgaag
     9961 aacgcataac taatttatta gaaataattt tgctagaaat tgaatcatac aacacttgta
    10021 aaaatgatat ttcatgggaa aagttaaaaa gcgtgattag tgaagtgttg tataaaataa
    10081 taaaaaatga atcagaaaaa acagtatctt cagttagacg tggcgataca acgattagtt
    10141 atgcttcaac aacaaatgat gttagtgagt tacttctagg ctatggtgat ttgatacgaa
    10201 gagttattgg ttgtggagga ttggagtttt tttaatgaat gaagcagata ttttagaaac
    10261 tacttatgaa gatagttgta ttattgaaag actgacggac attgaagatt ctaatacaaa
    10321 tattactatt caagattaca aaaaagtata tgataatcct atttcttgcg ctctttcaca
    10381 aggtcaaatc gatggactag cagtcataga agatggagag atggtaaatg tttcaactga
    10441 cacatataaa ttatttgttc atcctaagat taaactaaaa aaaggagatc gaataacaat
    10501 aactcaaaag gccagtggct taattttttc tctatttgct actaagcctt tttactatcc
    10561 tagtcattgt gaagtgaatt tgataggaag tgagaaaaat gggtaatctc aaatatgaat
    10621 caaatgcaga aaagatcatt gagaatttta aaaatatgac tgtaattgct caaaaagaag
    10681 gaatatcctt cgttaacgat tcaatgaata aagtcgttag tttagctaaa ccattaactc
    10741 ctgtaaaatc aggtaattta aggcgaggat atcgtgtagt aaaagctaga aaactatcaa
    10801 gcggtcgaat tgttggagca gttataaaca atgaacatta ttttaaatat gtagaagaag
    10861 gtcataggac taaaaatggt ggctttgtca aagggaaatt catgttaact cgtgcaacga
    10921 atcttgcaaa tatgtcttat attcctcgaa gatttaaaca aatggcaata aaaatcgtta
    10981 agaaaggaaa gtaacatgaa agatgaaatc attgctgcaa tcagtagcaa attaaaagaa
    11041 atctatcctg atgggacaat atatcttgat tcagttatgc agtcaactaa agatttttat
    11101 ttcgttttat cagtaatgga atctggaact gaaaatgtag gaattgatat tcaaaatgtt
    11161 tctttcttaa ttgatattgc attgattgat aataaaccta atagaaattt aattaatgaa
    11221 ttagtctcac gttgtgggac tttttttaat acgattacaa tcgatgagca aatattattt
    11281 ccaaaagcct atttacctga tgaagcagat ggtgttcaac atattcgttt tacattagaa
    11341 tttccacaat atattgaatg gagtgaaaga taaatgggag aaaaaagaag taaaaccgga
    11401 attatttctg tcgaaaaacc tacttggttt cctttggaag atgagacagg gaattttccg
    11461 gtatatggta ctgcgttgcc aatgggaaca gcagtaagta tcaaaccaac agctaattat
    11521 gaaacaacac aagattatgg ggattcagtg gttcaagatc aatttacagc ttttggtggt
    11581 gcagaagttg aactggaagc aaatggatat actcataaag ttttatctac aattactgga
    11641 gggaaaattg ttaaaggtgg tgccttgcgt tctggagaag atattgcgca agatggtgcg
    11701 tttgcatata gacgtaaaaa atcgaatggg aaataccgtt atacggtctt ttataaagga
    11761 cagtttgctc tagattcaga tgaaacatca acgattgaag gaagtaaagt aagttttact
    11821 catccaactt ggaagggttc atttgtagat gtaccaggac ttggatatat gtattcagtt
    11881 gatgaagatg atgaaggcgt ggataaagca atgatcggaa attggtttac aaaagtagca
    11941 attccaattg aagaagcaga actttcagga ggtacagaat aatgtcaaaa tatcaaacaa
    12001 cgattaaatt aacgaaaaaa gatgaagaag gtaaatatga gcaagtacaa tttaaatctg
    12061 ctgaattttt accaggaaca gttgtagaag atgctgcagg agttatggaa gaaatgcaaa
    12121 cagcgactga taaacaatca gttaaaaaag ctttaagtcg tgcttattca tttattgcag
    12181 atactttatt tgagggacaa tttactggtg aagattattg caaaggaatt gatgctcgtg
    12241 agattgcctc attgacagga aaattattga agtctgttac tgcaggtttt gatgaaactt
    12301 atacagagac gaaaaaaaag taagtgaggc gctcaagtca ccttcattta agtattcgat
    12361 tacttaccga gaattagata taaagacgca attactcgaa gcaggttgga cgttaccaga
    12421 aattgaacgt actgacttgg atgagttaat gcgtcttttt gcttttagag atgcggtaaa
    12481 agagcatgaa gatgttgagt actatgataa tttcactcaa ttttaggagg tgatactttg
    12541 aataacgatg acctaattct gaagatgata ctagatgaat ctggttttac tgccggtatg
    12601 aataatgctg tcaaaaagtt gaactctttt gatgaaacaa tcgaaagaaa aagtagaaat
    12661 agtggcagtt cattaggtag tatttggaaa atatttgctg gaagttttct agctagcgga
    12721 gtaactagaa tcgtaggagc tggctttgat ctaatcaagg gttccataag tggagcggtt
    12781 gatcgagtag atacgatgaa taacgctcta cgaaatttcc aaaatatggg atttagtaat
    12841 tcagaaatta tgaagaatat cggaaagaat gggcttttat ctcaaggtat tcaaggactt
    12901 cctactgctt tgaatgatgc gataagtcat gttcaacttc ttgcttcttc tacaggtgat
    12961 ttaactcgtt caactcaaat atttaaagct ttaaatgatg gaattcttgg tttcggtggt
    13021 tcaactgatc aagttaatga agctgttatt caattatctc aaagtttctc aaacggaaaa
    13081 gtagacgcac aaacttggaa ctcaatgatc aacgctcaac ttggacctac tctttctgct
    13141 attgctaaaa agatgggaat tacaatggga gatctgaaag aaggtttgtc tcaaggtaag
    13201 atttctgttg aagaattcca aaatcaatta atagaaatgg ataccaaagg tggcggagga
    13261 cttaaatcat taagtcaaat cgctaaagat tctactaagg gaattaaaac ctctatacaa
    13321 aatgcaaaaa cagctgttac acgtggtgtt ggtgaggtaa tagaaggatt aaataaagca
    13381 ttagtagatt cagatttagg tggatttaag ggaattattg ataaagtggg cagttcaatg
    13441 gaatcgttct taaaagtaat tgctgcaaat attcctacag cagtatcatt tttaagtaat
    13501 ttatttaacg aaattcaaaa atttggttct gctttgaaat tcatgatgcc ctttcttgtt
    13561 cctgcagcta ccgcttttgg agcgttcatg tttcaactta aaggtatacc agcaataata
    13621 aaaagcttca ataattttaa gaatgccata attggcgttg gaaattcatt aaagattatg
    13681 ggggcaatag ctgctgctaa ttcatttgtt ttaattattg gtgcagtcgt tggtgcaata
    13741 gctgttttcg gttactttat ggcaaccaat gaagagttta gaaacaaggt tatatctatt
    13801 tggaatgatg tgaaagattc cgtaattggt gtattaaaaa atataaagga ctggggaatt
    13861 gatacttgga attctgctaa agaaatggca tcaaatgcag ttgagggtgt caaagatgct
    13921 tggtcaggaa caaaggaatg gttttctaac acctggaaag gtattaagga tggggccaca
    13981 ggtttatttg ataaaacagt agagacttca agaaatgcag tcgatagtgt aaaaaatgca
    14041 tggtctaata tgaagaaatg gttttctgat acttggcaaa gtataaaaga ttcagccaga
    14101 gaaaaatgga atgaaattaa aggttctatt atgtcggttg ctggtccatt gattacaggt
    14161 ataaagaatg catttttaca tgttactttt tatctagaga ccttatggaa taatttagtt
    14221 gagattggta aaaacgtctt tgagatttta aaaaatgtta tacttgcgcc ggttttgttt
    14281 attacctcca tgatttctgg tggatgggaa gaaacaaaaa ataatatgat tgctgtttgg
    14341 aataatatta aagaaagtgc cataaatatt tgggaatcta taaagaatat atttgtaagt
    14401 tattttacaa atatttattt tgctgcactc aatatttgga cagggttcaa gcttacattg
    14461 ataaatattt ggaatgaagt ggtaaatcaa gctaaatcga tttggttaaa cataaagtat
    14521 ttttttatta atctttggat tgatataaaa tattttgcaa ttcagaaatg gattgaattg
    14581 aagttttcta ttattcaaac ttggatcgat ttaaaatata atgccattac tacttggaat
    14641 aatattaaac agtttttcaa agatacttgg aaaaatatta aagatacggc atacaatacg
    14701 tggatttcta taaaaaatac catgattaat acatggaata acattaagga ctctttctgg
    14761 aatattgtta ctggaattgt taattctgct gaaaatgcat ggacaaatct aaaaaatggt
    14821 gtttcaaatg ctattaatcg ggtaaaagaa atctttaatt cgttaaggga agtcaattta
    14881 tttgaaattg gcaagaacat cattgatgga cttataaatg gtgtaaaaga aaagtggaat
    14941 gctttgaaag atactatcaa aggtattgca ggtaatatca aaaattggat taaaggagct
    15001 ttagatattc attctccatc aagatggatg cgtgatatgg ttggtaaaaa tattgttcaa
    15061 ggtattatcg ttggtattga taaagagcaa agtaagttag atcaaaccat gacaaattta
    15121 gtaaaaacac cgtctgttca acctgtagta aatggaataa gtcacctacc aattacacaa
    15181 acaaagcaaa atatatctgc tagtgaaaca aaagaaattc atttacattt aaatgtttat
    15241 ggagatttac ctgattcaat ggttcggaag attaccacaa aaattaaaac agaattgaca
    15301 agacaaatga aacgagatgc tggtgcagta ggaggaacat tatatgcaac ttaagagagg
    15361 acaatttttt attaatcaac attattcttc tgaatttaat gtgtatattc aaaacaggcc
    15421 tgcttctgtt tcagctagtc gagtaattga attgagagag cgagaaggaa atgattctat
    15481 cattattgat aaaggctatt acagaaatgt gactagaaag attgagtgtt actataaagc
    15541 accttcaatt gatgtggtgc aggagtggga agatcgaatc actgaatggt tagatatgtc
    15601 gtcttatagt gattttattc tttactatga tgaacaatat atttatcaag ctgtagtaat
    15661 agaaggacct gaatttaaag gaacaagaaa aacaggaaat attgtccctt ttgaatttac
    15721 agtgagcatt cgaccattta aggaaaatta caatggtaga tttactattc gacaaacaga
    15781 aacttttgaa atttataacc cagagaagta ttcttcaaaa ccgcttatta aattgagtgg
    15841 ttctggagat gcttcttttt atattaataa ggacaaatat gatttgaaat cgttggacag
    15901 agaattattt atagattcta aattagaaga ggcttaccga aaattagatg gtaatttaga
    15961 acatcaggat caagtcactt tatttttaga ttttccattt ttatatcctg gaaaaaatga
    16021 aattaaatgg acgaaaaata ttcattcatt tgaaataatg cctaggtggt ggagaaaagt
    16081 atgaaaccaa gaatatatag tcctactgaa acagatttct caacgaatgg cttaggaatt
    16141 ttaaaggatt gtacaagatg tgaaatatat gaagtagcta atggaaaata tgaattagag
    16201 ttggattatc ctttaggaac tagatttgat gaatattttg aaaatgacta tcaaataaaa
    16261 gcaaagccaa acgatcaaga agagtatcat atctttttta ttgatgataa agatatagat
    16321 acttttttaa atacagtaac tatttatgct cagagtcgta caaatcgact tggaagacgg
    16381 gtagtcactc ttgcggaagt agactctaaa actggtcaag aagcgatatc aattattgaa
    16441 accaaaatgg ataaaaaatc tgacatacga ctttattctg atattacggc cgtttctagc
    16501 acaatctttg aagcgagaaa tgttttaaat tgtattgctg gtgaacaagg atcattgctt
    16561 caatattggg gtggagaaat taaacgtgaa ccatttaagc tttctttgtt aaagcgaaga
    16621 ggtcgcaata atgttggaac gattcggtat ggaaaagata tgtctggttt aaaggtcaaa
    16681 ttagattgga caggtataaa aacaagactt attccatatg ctgatcctca aagtgaggta
    16741 ggtacgacta atcgaattta tggttcgcca gtagatagtg cctatattaa taactatcct
    16801 gatgtgtata cagagcatgt tcagtttaca gaagaacaag gagtaaaaga cattaagagc
    16861 ttaaataaaa tagctaaaaa ttactttaaa acgatcaatc ctggctgtga taagcctaaa
    16921 gtttctatta cggttgaatt tgataagttg actgatagtg aagaagcgaa agaatttgcg
    16981 aagattagaa attatggtct gtttgataca tttaaaatat atcataaaaa atataatatt
    17041 tatcttgaat caaaggttag tggactacaa tacgattctt tatctgaaaa aactttgaaa
    17101 ttagaagcag gagacattca agttgctttt tatcagcaac aagctgtaac tattcaggat
    17161 aaattaaaag attatgcaac gaataattat atgagtgatt ttaatgatta tgtttcttca
    17221 atgattacag gtcaaggcag tgcaggagga tatgtaagtt tatggccaaa agaaaaacct
    17281 tccaacatct ttattatgga taatccagat ttagaaaagg caagagaagt actaagaatg
    17341 aataaaaatg ggattgcttt ttctaagaaa ggatggcggg ggccttttac ttcagcttgg
    17401 acattagata gtatatttaa tgccaatttt atacaaactg gattaatcaa agcagatatc
    17461 tttcaaaatt cttttaacaa gactggagat gtattgaaat tagttaatgg actactccaa
    17521 atttggaaca acaaaaagaa aataatggag ttaactaaaa aaggaatgga attttggaat
    17581 tctaagggtt caattggaac gattggaaca actgattctg ctggtaatcc ttttcctggg
    17641 gcttctactc caacccctat tgaagataat tctttagtta ttcgtacaaa tggagacggc
    17701 aaatatattt tgatttctcc taaagtagga aaaggattag ttttattagg aaatggtaaa
    17761 gcaatttatt ttggagactt agatgtacaa ggaaaactta cagttaaagg aaaagaaata
    17821 attcctggac aaggtggtgg tccatcggga ggtggagaat ctactggtgg gtatcctagt
    17881 gaattaaaaa cagatgcaga aaagagagct tggagaattt acgatatttt gtgtaataac
    17941 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
    18001 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
    18061 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
    18121 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
    18181 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
    18241 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
    18301 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
    18361 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
    18421 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
    18481 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
    18541 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
    18601 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
    18661 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
    18721 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
    18781 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
    18841 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
    18901 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
    18961 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
    19021 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
    19081 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
    19141 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
    19201 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
    19261 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
    19321 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
    19381 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
    19441 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
    19501 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
    19561 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
    19621 caagacgatc tcttttcaga taatcacgaa gtcgaaaaag ttggaactgt agaattcccg
    19681 attgaagaag gagccttgat catcgcaacc gttgatgatc caaagcaaaa tgtgttcact
    19741 cttgagaaag tcggggtgat ctaatggcta aaactaaacg aatgatggaa acggatgaaa
    19801 aaacaggtgt acaacgccaa ttctttccaa tcacacatgc ttccgcagtt cttgggttag
    19861 aagaaataat agcaggagaa gcaacagttt tatctgttaa tggaaaaatt ggagccgtcg
    19921 tcattactaa agaagatttg ggattagaga atgttctcac agaattaccc tatgcaagtg
    19981 aagaagatga cggcattatc acggcagaaa tgtatcaaaa aattttaaac agtggagaag
    20041 gtgactacgt gttgccagtt gccactatcg accgtttggg tggcataaaa gttggtgaat
    20101 tattgacgat tgatgagaca gggaaagtct ctgcagtcag acaatctgat gtcaattttt
    20161 cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta tactgctgga gaaaacatca
    20221 ctattgatga agatggaaca attagttcta caggtggtag tgggactggt ggtgtcaatc
    20281 agtcatatgt agatcaaaaa ttccaagaag ctgtaaatca agcagaaaat tacacaaacg
    20341 aaagaattcc aaattttact tttgaaaaaa ttggggaggt atagagaatg acagatatcg
    20401 taaaaataaa acaaagcaat gtacaggttt atccacaaac tcattggaat gctatagaag
    20461 gcaaaccaac aacggtgaag ggagataaag gcgatcctgg ccaagcagca acaattacca
    20521 taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa tgtaggaaca tcatctgcag
    20581 ctagatttaa ttttgtttta ccaaagggag ataaaggtga tcctggaata aatgccacaa
    20641 ctacagctgt agctacaact acagcaaatg ggttgatgtc gtccacagat aaaactaagt
    20701 tggatggaat tgcagctgga gcacagaaaa atccaggtaa tgctacaact acgacagcag
    20761 gtttaatgtc agcaaccgat aaagtgaaac ttgatggatt agccaatatt acatttgaga
    20821 aagtggggac ggtttaatgg ctgatattgt tcaattaaaa gaagacggag ttgctaaata
    20881 cttaaaaacg catgtaaaag ctattgatgg taaagaagca ttggtgcaga cagatggaga
    20941 ccaagccatt gcaggacata agaatttttc aggttctgta acaatcaata ataaacgtgt
    21001 tttgacgact gatgataaca gatatgaagt ggtaaatctg gttgtcacta acggtaatac
    21061 agggacagca aagctttatc gtgaaggaaa aacagtttct atatattttg tggctttaaa
    21121 cggaaaaagt agtggcggga atgattcggt tattttaact gttccagaag gctatcggac
    21181 accaattagt tttgaacaac tggttgggtc aatagaccgt tctactttga acagtgctca
    21241 actatctatt ggcgcagacg gagccattaa atggagaaga aattcaagtt atggatcagc
    21301 ttattcattt gttatcactt attcaattta agggaggaaa tctaatgaaa gtagtttaca
    21361 aatcaatcaa gccttacgga ttcgagcaaa tcattttgaa caatcaagaa aatatccctg
    21421 aaaactgtac agagattaaa ccaccagttc ctaactggag accaagattt gattttgata
    21481 aaaaacagtg ggttgagtta gctactgaag aagagaagag tggcacagcg gttgacgata
    21541 ttgaagatgt cgatcagttg aagcaattaa atgctctact gacaaaacaa ttggcgatat
    21601 cggtacagga acaagaaaaa atgcaacaaa tgttagctca attgacgatg gaagtcgcaa
    21661 gtattaagaa tggagggaaa agtaatgaat agttttccgg gctttgaaaa tataaaacaa
    21721 ttttatgatt ggggatgtta tacggaccaa gatttacttg actatgtaaa catgaactgt
    21781 ttaacaaaag accaatataa gcagattact ggaaatgaaa tttaattaag cacaaagtta
    21841 aataaaaagc gtactcaaac gagtgcgctt ctttgcataa aggagaaata gatattggaa
    21901 aaatatttaa atacactctc agtaatgaca ggggtgatag gcgggacaat tgttggttta
    21961 ttgggaggga tggacaatat actacatgtt ttaatttttc ttgtgggtgt ggacttctta
    22021 actggacttg ctaaggcatg gaaattgaaa gaaataagta gtgagatagg tttcgaagga
    22081 ttgttgaaga aagtcttaat cttcgttgtg attgccgttg cggttgacgt acagaaaatc
    22141 gttgggaatt ctatacctct aagagaaata gttattatgt tctatgttgc aaatgaaggg
    22201 attagttttt tagaaaacat ttcggtattc cttcctttgc cagataagtt aaaagaggta
    22261 ttccaacaaa tacgaaatga tactgaaaat aaagaccgag gaggaaccaa atgaaaaaga
    22321 aaatctttgt aggagctatt atagctcttt ttttattgcc aataaacgcc tttgcttact
    22381 cgatcaacaa tgaattcaat ttgggcgcaa acgaaggtag ttctcaagta gcaaataacc
    22441 aatacatcct attgcatgaa acagcaaacg aaactgcgac aggacgaaat gaagcgcagt
    22501 acatgaaacg ttcttggtac aatgcctata cagcgtatat tgttggcgat ggtgggatcg
    22561 tttaccaagt tggacaacct ggttatgtac aatatggcgc tggttcatat gctaatgcaa
    22621 atagtcctgt gcaaatcgaa ctgcaacata cgcacgataa agtaacgttt gaaaagaact
    22681 acaaggcata tgttgaatta gcgagagatt ccgctaataa atacggtatt ccacttacat
    22741 tagacacacc gtataatcaa ccaggaatca aatcacattt atgggtaact caaaacattt
    22801 ggggcgatca cactgatccg tatggatact tatca
//
LOCUS       DACEDM010000051        22240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEDM010000051 DACEDM010000000
VERSION     DACEDM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.051"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            171..515
                     /locus_tag="IWO50_001820"
     CDS             171..515
                     /locus_tag="IWO50_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0358944.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IWO50_001821"
     CDS             516..935
                     /locus_tag="IWO50_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0358945.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1027..1377)
                     /locus_tag="IWO50_001822"
     CDS             complement(1027..1377)
                     /locus_tag="IWO50_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0358946.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IWO50_001823"
     CDS             complement(1343..2674)
                     /locus_tag="IWO50_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0358947.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IWO50_001824"
     CDS             2930..3496
                     /locus_tag="IWO50_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0358948.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IWO50_001825"
     tRNA            3641..3728
                     /locus_tag="IWO50_001825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /locus_tag="IWO50_001826"
     CDS             4062..5036
                     /locus_tag="IWO50_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0358949.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="IWO50_001827"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="IWO50_001827"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0358950.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="IWO50_001828"
     CDS             complement(7451..7897)
                     /locus_tag="IWO50_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0358951.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="IWO50_001829"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="IWO50_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0358952.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="IWO50_001830"
     CDS             8362..8880
                     /locus_tag="IWO50_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0358953.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="IWO50_001831"
     CDS             complement(8957..9913)
                     /locus_tag="IWO50_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0358954.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="IWO50_001832"
     CDS             10079..10885
                     /locus_tag="IWO50_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0358955.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="IWO50_001833"
     CDS             10882..11871
                     /locus_tag="IWO50_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0358956.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="IWO50_001834"
     CDS             11868..12872
                     /locus_tag="IWO50_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0358957.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="IWO50_001835"
     CDS             13004..13546
                     /locus_tag="IWO50_001835"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0358958.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="IWO50_001836"
     CDS             13566..14264
                     /locus_tag="IWO50_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0358959.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="IWO50_001837"
     CDS             14285..14497
                     /locus_tag="IWO50_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0358960.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="IWO50_001838"
     CDS             14497..15459
                     /locus_tag="IWO50_001838"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0358961.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="IWO50_001839"
     CDS             15477..15872
                     /locus_tag="IWO50_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0358962.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="IWO50_001840"
     CDS             16035..16400
                     /locus_tag="IWO50_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0358963.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="IWO50_001841"
     CDS             16397..18208
                     /locus_tag="IWO50_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0358964.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..18437)
                     /locus_tag="IWO50_001842"
     CDS             complement(18270..18437)
                     /locus_tag="IWO50_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0358965.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18685..19674
                     /gene="galE"
                     /locus_tag="IWO50_001843"
     CDS             18685..19674
                     /gene="galE"
                     /locus_tag="IWO50_001843"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0358966.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19686..21170
                     /gene="galT"
                     /locus_tag="IWO50_001844"
     CDS             19686..21170
                     /gene="galT"
                     /locus_tag="IWO50_001844"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0358967.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21194..22174
                     /locus_tag="IWO50_001845"
     CDS             21194..22174
                     /locus_tag="IWO50_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0358968.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7212 a         3802 c         4630 g         6596 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggcaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaagaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attattcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 cacacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcaac caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatct cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt tagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga ttttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaattga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaaaga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tctcgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcca gacttcctac taatattcca
    18421 gatacaaatt ttttcattga atccacctca tttattattt gttatatcta ttatggaaca
    18481 aaagagaaaa aaaggctagt tttacgtttg tttgtaaacc tttgcaaaaa atccttgaaa
    18541 atgtttacta aatgttttat tataaattta ccatatgaat ttacgaaatt ttactttgtt
    18601 tgttttcacc ataaaagtaa acttcgtttt ttaaattata tagaatcaac aaacacaatt
    18661 ttactaatca aaggagtgtt ttttatgaca attttagtac taggcggagc cggttatatc
    18721 ggttcgcacg ctgtagacca gctagttcaa aaggggtatc aagtagctgt ggtagataat
    18781 ttgctcacag gtcacaaaca agcagtacat ccagatgctc atttttatga aggagacatc
    18841 cgggataaag aatttctacg ttccgtattt gaaaaggaac cgattgaagg agtaatccat
    18901 tttgctgcaa gctctttagt aggagaatct gtcgaaaaac cattgatgta tttcaacaac
    18961 aacgtatacg gtatgcaaat cttacttgaa gtcatgcacg agttcaacgt gaataaaatc
    19021 gtattctctt ctactgcagc aacttatgga gagccgaaag agtctccaat cacagaagat
    19081 acaccagcta atcctaaaaa tccgtatggt gaaagtaaat tgatgatgga aaaaatgatg
    19141 aagtggtgtg accaagcata tggcatgcgc tatgtcgctt tgcgctactt caatgtagcc
    19201 ggagcaaaag cagatgcatc tatcggagaa gatcatacac cagaaacgca tcttgtccca
    19261 atcattttac aagtagctct tggtcaaaga aaagccttgg ctgtttatgg agacgattat
    19321 gatacaccag atggaacatg tatcagagat tatgttcaag tagaagattt gattgctgca
    19381 catattttag ctttggaata cttgaaagaa ggaaatgaaa gcaacttctt caatttaggc
    19441 agcagcaaag gatactcagt caaggaaatg ctagaagcag ctagagaagt gacaggaaaa
    19501 gaaattccag cagaaatagc accaagaaga gctggagatc ctagccgatt agtcgcttca
    19561 agtgaaaaag cgagagaggt tctgggttgg aaacctgaat acacagatat caaagcaatt
    19621 atcaaaacag catgggattg gcatgtcagc catccaaatg gttatgaaga ataggggaat
    19681 gagatatgtc aatcagtcaa acgatttgtg attttgccac acttgcgatc gaagctggcg
    19741 gatggatgga aatggaccgc ttatatttac agaatcgtct tttagggatg gtaggagaag
    19801 atgctctaga aagcattgaa gtaagaccag ttaaggaaga atctgtagac ttattggatc
    19861 agcttgtcgc taaggcaaga gaaaatggtg ttctttcaga attgccagca gaacaagaga
    19921 tttttgaagc gcaattaatg gattttctca caccgccacc ttcagtcgtc aatgcattct
    19981 ttgcacagca ctattcgaaa agcccagagg aagcaactga gtatttcttt aatttaagta
    20041 agaaaaatga ctacatcaaa acaagagcca tcgcaaaaaa catcaaattt ccggcagagt
    20101 cggagtatgg tgaactggaa atcacaatca atttatctaa gccagaaaaa gatccaaaac
    20161 aaatcgcagc agaacgcgag gcggtaaaag tagactatcc tacctgcatg ctttgcatgg
    20221 aaaacgaagg atatagaggt cgcttgaact atccagcccg caccaatcac cggattattc
    20281 gaatgaatct agacggtgaa agctggggat tccaatattc tccttatgct tattacaatg
    20341 aacattcgat cattttatca gaacaacacc gcccgatgaa aatcacaaag gaaacatttg
    20401 ctagactgct tcggattact gaagtcttgc ctcattactt tgttggttcg aatgctgact
    20461 tgccgattgt tggaggatcg attctttctc atgatcatta tcaggcagga cgtcatgaat
    20521 ttcctatggc aaaagcaccg atagaaaaac ttatcaaact agcagattat ccgcaagtca
    20581 tcgcaggagt cgtgaaatgg ccgatgtctg tgatccgttt gcaatcaaca gagaaaaaat
    20641 tgttgattga agcagctaac tcagtattgg aaaagtggaa agcatattcg gatgaaacag
    20701 tatctatcag agctttttca gcagatggta caccacacca tacgattacg ccaattgcac
    20761 gaagaaggga caattacttt gagttggacc ttgtccttcg agacaacaat gtttcagaag
    20821 aacatcctga tgggattttt catccacata aagatgtcca acatatcaaa aaggagaaca
    20881 ttgggttgat cgaagtaatg gggcttgctg ttttaccacc tcgtttgaaa ccagaactag
    20941 tagaagtaga gcatttctta ttaggagaag aaaaccagat ggctgaatat catcgtccgt
    21001 gggcagaaca attaaaaaaa gaacatcctg atttgacgaa agatacggtc accgatgtcg
    21061 tacaaaaatc tgtaggtcag gtttttgcac gtgtactgga agacgctgga gtattcaagc
    21121 gagatgaaaa aggacaggca gcgtttgaac gatttactgc aattttataa acgtaaaaaa
    21181 ggaggagaga caaatggcaa caattaaaga cattgcgcag ttggcaggag tttctcctgc
    21241 tactgtttca agagttctca actatgatcc tgatctttcc gttggcttag ccacaaagca
    21301 aaaaattttt gaagcagcag aacgcttaaa ttatacgaaa catcgtaaaa atgcagcaaa
    21361 tactaagcaa acgcttcgcc tgatccagtg gtacgacgat acggaagagc ttgaagatct
    21421 ttattattta tctattcgtc taggaatcga aaaaaaggca gaagaagcaa acgttacttt
    21481 attgaaagaa acgtgggatt ccattagtga tcttccgaca gatggaacga tcgcactagg
    21541 aaaattcgac gaagctcagt tattagaaat gaaaggatcc caaaatgcct tgcttttggt
    21601 agattctgac ggcacttctc aaggaatcga ctctctagtt gtcgacttta agtcaagtgt
    21661 tcaaaaagtc attgaacatt ttgtctccca taaagcgcaa catatcgcta tgcttgctgg
    21721 aacagaatat acgaagaaaa accatcagcg gctaaaagat cccagaatta tggcatttca
    21781 acaagttttg gcagaaaaag gctacgacga aggtccaatt atcgaagcag atttttctgt
    21841 cgagtcagga tatcaagcgg tgaagcagta tttaaaaacg aatccgcatg ttcctgatgc
    21901 gctttttgca gctaatgatg cattagcgat tggcgcactg aaagcgattc aagaaaaagg
    21961 tttagtggta ccagaagata tttctgtgat gggtttcaat gatgtcagtg tagcgaaata
    22021 tgtaacgccc tctctttcta ctgtaaaagt ttataccgaa tggatgggag agctagcagt
    22081 ggagacgatt cttcaattaa gtaattccaa agcaccggtt tcaagaaaga ttattgtgga
    22141 aacagagttg attattcgag aatcaacgaa ataaaagatg gaaaagctgt ggggaaatga
    22201 agtgataccg cagctttttt gtgtttcagg aaggggcaaa
//
LOCUS       DACEDM010000052        22163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEDM010000052 DACEDM010000000
VERSION     DACEDM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.052"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(241..795)
                     /locus_tag="IWO50_001846"
     CDS             complement(241..795)
                     /locus_tag="IWO50_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0358969.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(921..2849)
                     /locus_tag="IWO50_001847"
     CDS             complement(921..2849)
                     /locus_tag="IWO50_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0358970.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3155..3871)
                     /locus_tag="IWO50_001848"
     CDS             complement(3155..3871)
                     /locus_tag="IWO50_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0358971.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3872..5695)
                     /gene="glpO"
                     /locus_tag="IWO50_001849"
     CDS             complement(3872..5695)
                     /gene="glpO"
                     /locus_tag="IWO50_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0358972.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5709..7205)
                     /gene="glpK"
                     /locus_tag="IWO50_001850"
     CDS             complement(5709..7205)
                     /gene="glpK"
                     /locus_tag="IWO50_001850"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0358973.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7341..8801)
                     /locus_tag="IWO50_001851"
     CDS             complement(7341..8801)
                     /locus_tag="IWO50_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0358974.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8791..10086)
                     /locus_tag="IWO50_001852"
     CDS             complement(8791..10086)
                     /locus_tag="IWO50_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0358975.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10418..11095)
                     /locus_tag="IWO50_001853"
     CDS             complement(10418..11095)
                     /locus_tag="IWO50_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0358976.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11125..12216)
                     /locus_tag="IWO50_001854"
     CDS             complement(11125..12216)
                     /locus_tag="IWO50_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0358977.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12317..12751)
                     /locus_tag="IWO50_001855"
     CDS             complement(12317..12751)
                     /locus_tag="IWO50_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0358978.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13335..14792)
                     /gene="ascB"
                     /locus_tag="IWO50_001856"
     CDS             complement(13335..14792)
                     /gene="ascB"
                     /locus_tag="IWO50_001856"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0358979.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14789..16684)
                     /locus_tag="IWO50_001857"
     CDS             complement(14789..16684)
                     /locus_tag="IWO50_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0358980.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16742..18517)
                     /locus_tag="IWO50_001858"
     CDS             complement(16742..18517)
                     /locus_tag="IWO50_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0358981.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18567..20063)
                     /locus_tag="IWO50_001859"
     CDS             complement(18567..20063)
                     /locus_tag="IWO50_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0358982.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20257..21879)
                     /locus_tag="IWO50_001860"
     CDS             complement(20257..21879)
                     /locus_tag="IWO50_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0358983.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
BASE COUNT         6273 a         4839 c         3646 g         7405 t
ORIGIN      
        1 agagaaacaa acggaaaaat agctgctcat attttgccgt tttttgagct aataccgaag
       61 gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca
      121 caagaaataa tttcaaaaac aacttctcct gtttttggtt tggttggctt attatcgaga
      181 agtagctact gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt
      241 ttattttctt tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag
      301 accgtaagtg atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag
      361 cagcaaaagg atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc
      421 tccacttaat ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc
      481 ttttatcaag aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa
      541 aatcagaaaa agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt
      601 tacctgatta tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac
      661 aatgaccgct cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag
      721 caaaaaaata cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc
      781 tgtgcttaat ttcattttat cgccctacct cctcagcact cttttctttt tattttagct
      841 tacacgggga ataaaaccaa tgatttcact gtttttattt ttctttacag ccactgatat
      901 tatgagctaa tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt
      961 cgtttgcgca tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca
     1021 cgctgccgtt tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg
     1081 tacagatctt ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt
     1141 ttgtctaaat atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt
     1201 tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctggcgttc ttttttattc
     1261 tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct
     1321 ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga
     1381 gcaatccaca aagcttggct ttctcctgtt tctccaaccc gcaactgata tttaggacga
     1441 agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat
     1501 ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat
     1561 tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca
     1621 tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata
     1681 tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa
     1741 atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc
     1801 gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct
     1861 ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc
     1921 tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta
     1981 ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc
     2041 gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat
     2101 gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca
     2161 ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg
     2221 ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt atagtattcg
     2281 cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat
     2341 ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc
     2401 aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct
     2461 tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat
     2521 tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg
     2581 atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc
     2641 ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt
     2701 gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt
     2761 cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat
     2821 tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc
     2881 ccaaaataaa taagggccta gagataaata aaaggataga acacagctta aaaaagtcat
     2941 gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca
     3001 caccaaataa actttgttta ttggctattc gacttactga ttagatacca gctacactca
     3061 caaaacatac cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca
     3121 ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa tagacaccta
     3181 ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg
     3241 aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag
     3301 cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc
     3361 cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc
     3421 caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta
     3481 tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat
     3541 gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa
     3601 tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga
     3661 ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc attgttacag
     3721 cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta
     3781 aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact
     3841 ctcccatgat ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat
     3901 ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc
     3961 attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc
     4021 gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt
     4081 cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg
     4141 ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat
     4201 aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg
     4261 tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga
     4321 tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc
     4381 cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg
     4441 gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt
     4501 tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt
     4561 cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac
     4621 cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt
     4681 cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat
     4741 aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg
     4801 tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct
     4861 ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat
     4921 ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta
     4981 ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt
     5041 catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc
     5101 caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt ttgatattat
     5161 caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc
     5221 cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct
     5281 catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg
     5341 agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg
     5401 gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta
     5461 ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc
     5521 gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg
     5581 ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca
     5641 attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga
     5701 tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt
     5761 ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg
     5821 cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct
     5881 agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg
     5941 aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg
     6001 attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt
     6061 aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct
     6121 cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc
     6181 tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt
     6241 tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa
     6301 gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat
     6361 aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg
     6421 accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc
     6481 cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct
     6541 gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc
     6601 tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca
     6661 gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac
     6721 atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt
     6781 atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct
     6841 tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca
     6901 actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg
     6961 ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg
     7021 gcaataactg actgtacaga attccagatt tcatttgcat catgttccac ccaactttct
     7081 tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca
     7141 aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct
     7201 gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac
     7261 tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt
     7321 attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc
     7381 aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg
     7441 tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc
     7501 tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa
     7561 atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc
     7621 atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt
     7681 ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa
     7741 tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt
     7801 ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct
     7861 tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta
     7921 tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct
     7981 tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc
     8041 agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc
     8101 cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc
     8161 aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta
     8221 aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt
     8281 tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat
     8341 cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct
     8401 aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct
     8461 gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac
     8521 ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac
     8581 acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca
     8641 atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct
     8701 ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc
     8761 tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt
     8821 tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa
     8881 ctgttcaaac gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat
     8941 ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt
     9001 gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat
     9061 tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc
     9121 aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat
     9181 tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt
     9241 ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg
     9301 agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc
     9361 ttcccacatc tgattgtcgg ggcgagaatg attagccagc aaaacaagat catagttctc
     9421 ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc
     9481 ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc
     9541 aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt
     9601 tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat
     9661 tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg
     9721 gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag
     9781 aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa
     9841 tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt
     9901 cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc
     9961 gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat
    10021 caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat
    10081 gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac
    10141 acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct
    10201 ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag
    10261 agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc
    10321 ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg
    10381 tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat gcttcaacaa
    10441 ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa
    10501 tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag
    10561 catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac
    10621 tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca
    10681 acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg
    10741 aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag
    10801 cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca
    10861 aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca
    10921 aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga
    10981 tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag
    11041 gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc
    11101 catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg
    11161 gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc
    11221 acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc
    11281 tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac
    11341 accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc
    11401 gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc
    11461 tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg
    11521 tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg
    11581 ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa
    11641 gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa
    11701 atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt
    11761 ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt
    11821 ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa
    11881 agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc
    11941 tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct
    12001 gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc
    12061 accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt
    12121 gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc
    12181 tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc
    12241 tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta
    12301 atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg
    12361 ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc
    12421 agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca
    12481 agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag
    12541 caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt
    12601 tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg
    12661 cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt
    12721 catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat
    12781 agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat
    12841 ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc
    12901 tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat
    12961 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga aaaactggct
    13021 tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac
    13081 agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc
    13141 tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga
    13201 gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt
    13261 ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc
    13321 cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca
    13381 aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag
    13441 atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc
    13501 cacgacgtat aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata
    13561 tgctgtttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg
    13621 tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat
    13681 tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga
    13741 agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct
    13801 ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag
    13861 gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac
    13921 attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa
    13981 gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga
    14041 agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt
    14101 gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa
    14161 tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg
    14221 atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca
    14281 gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc
    14341 aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga
    14401 cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca
    14461 ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca
    14521 gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga
    14581 taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc
    14641 cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca
    14701 gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg
    14761 ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc
    14821 ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa
    14881 caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca
    14941 tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc
    15001 catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga
    15061 ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acaggactgt
    15121 agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg
    15181 ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta
    15241 tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac
    15301 aggaactgat catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat
    15361 aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac
    15421 cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt
    15481 taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag
    15541 ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc
    15601 ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag
    15661 aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt
    15721 gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat
    15781 atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact aataaagtaa
    15841 ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca
    15901 agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa
    15961 taaccgtact tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc
    16021 tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga
    16081 tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat
    16141 agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata
    16201 aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc
    16261 ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt
    16321 tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata
    16381 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    16441 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    16501 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    16561 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    16621 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    16681 ccattcaatt agtacccctt ataatagcga acgaaactgt acaatagtgt gtatggttta
    16741 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
    16801 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
    16861 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
    16921 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
    16981 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
    17041 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag
    17101 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
    17161 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
    17221 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
    17281 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
    17341 ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat
    17401 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
    17461 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
    17521 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
    17581 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
    17641 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
    17701 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
    17761 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
    17821 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
    17881 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
    17941 ttgtactgat ttgttgaggc aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
    18001 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
    18061 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
    18121 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
    18181 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
    18241 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
    18301 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
    18361 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
    18421 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
    18481 agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac
    18541 aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta
    18601 ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag
    18661 cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc
    18721 cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa
    18781 ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt
    18841 ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact
    18901 atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga
    18961 ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa
    19021 ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga
    19081 taataactaa atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct
    19141 aacattactg gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc
    19201 cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat
    19261 acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata
    19321 cccatcttag catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga
    19381 aacatatgat gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt
    19441 ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta
    19501 ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc
    19561 tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct
    19621 accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga
    19681 tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg
    19741 tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac
    19801 tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa
    19861 agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga
    19921 atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg
    19981 tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt
    20041 tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga
    20101 agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa
    20161 aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc
    20221 gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc
    20281 ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac
    20341 aataaccctt aaagcttcac gtggaacagt gaacgtctgc ttttcactgg acatattaca
    20401 aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt
    20461 cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat
    20521 caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat
    20581 ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc
    20641 acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcat tgattgattc
    20701 caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc
    20761 aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc
    20821 ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact
    20881 ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt
    20941 attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac
    21001 ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg
    21061 atctaatcct atgtgttcga attggaagac catggataat tcatttcttt taggattgga
    21121 ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt
    21181 gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact
    21241 tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc
    21301 aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt
    21361 tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt
    21421 ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc
    21481 attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc
    21541 ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg
    21601 gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc
    21661 tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta
    21721 tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat
    21781 aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat
    21841 ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga
    21901 tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt
    21961 tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa
    22021 attccctcaa ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa
    22081 cttctttata aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa
    22141 tatgaggaac tattttttcg ttt
//
LOCUS       DACEDM010000053        21832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEDM010000053 DACEDM010000000
VERSION     DACEDM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.053"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO50_001861"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO50_001861"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0358984.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO50_001862"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO50_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0358985.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO50_001863"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO50_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0358986.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO50_001864"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO50_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0358987.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO50_001865"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO50_001865"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0358988.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO50_001866"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO50_001866"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0358989.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWO50_001867"
     CDS             complement(8053..8523)
                     /locus_tag="IWO50_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0358990.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWO50_001868"
     CDS             complement(8569..8994)
                     /locus_tag="IWO50_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0358991.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWO50_001869"
     CDS             complement(9061..9330)
                     /locus_tag="IWO50_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0358992.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWO50_001870"
     CDS             complement(9341..10936)
                     /locus_tag="IWO50_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0358993.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO50_001871"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO50_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0358994.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWO50_001872"
     CDS             complement(14489..15049)
                     /locus_tag="IWO50_001872"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0358995.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWO50_001873"
     CDS             15402..16376
                     /locus_tag="IWO50_001873"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0358996.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWO50_001874"
     CDS             16546..17946
                     /locus_tag="IWO50_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0358997.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWO50_001875"
     CDS             complement(18024..18746)
                     /locus_tag="IWO50_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0358998.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWO50_001876"
     CDS             complement(18825..19331)
                     /locus_tag="IWO50_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0358999.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWO50_001877"
     CDS             19454..21253
                     /locus_tag="IWO50_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0359000.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..>21832)
                     /locus_tag="IWO50_001878"
     CDS             complement(21321..>21832)
                     /locus_tag="IWO50_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0359001.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6049 a         4789 c         3962 g         7032 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtca atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tg
//
LOCUS       DACEDM010000054        21662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEDM010000054 DACEDM010000000
VERSION     DACEDM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.054"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..351
                     /locus_tag="IWO50_001879"
     CDS             <1..351
                     /locus_tag="IWO50_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359002.1"
                     /translation="YLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLAWATRQSYETLL
                     SLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRLLFTDKATADAA
                     ALLVNGTVEEVVER"
     gene            348..644
                     /locus_tag="IWO50_001880"
     CDS             348..644
                     /locus_tag="IWO50_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359003.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            721..1134
                     /locus_tag="IWO50_001881"
     CDS             721..1134
                     /locus_tag="IWO50_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0359004.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1952..2296
                     /locus_tag="IWO50_001882"
     CDS             1952..2296
                     /locus_tag="IWO50_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0359005.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2301..2582
                     /locus_tag="IWO50_001883"
     CDS             2301..2582
                     /locus_tag="IWO50_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359006.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVRESLNKDNNVERIKNIFGYGIEEINYYIDFAEAFIPMVR"
     gene            2687..3001
                     /locus_tag="IWO50_001884"
     CDS             2687..3001
                     /locus_tag="IWO50_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0359007.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2979..4673
                     /locus_tag="IWO50_001885"
     CDS             2979..4673
                     /locus_tag="IWO50_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0359008.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4693..5871
                     /locus_tag="IWO50_001886"
     CDS             4693..5871
                     /locus_tag="IWO50_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0359009.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5834..6520
                     /locus_tag="IWO50_001887"
     CDS             5834..6520
                     /locus_tag="IWO50_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0359010.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6520..7680
                     /locus_tag="IWO50_001888"
     CDS             6520..7680
                     /locus_tag="IWO50_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0359011.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7690..8565
                     /locus_tag="IWO50_001889"
     CDS             7690..8565
                     /locus_tag="IWO50_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359012.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8562..8873
                     /locus_tag="IWO50_001890"
     CDS             8562..8873
                     /locus_tag="IWO50_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359013.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8863..9216
                     /locus_tag="IWO50_001891"
     CDS             8863..9216
                     /locus_tag="IWO50_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0359014.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9206..9607
                     /locus_tag="IWO50_001892"
     CDS             9206..9607
                     /locus_tag="IWO50_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359015.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9600..10004
                     /locus_tag="IWO50_001893"
     CDS             9600..10004
                     /locus_tag="IWO50_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359016.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            10016..10597
                     /locus_tag="IWO50_001894"
     CDS             10016..10597
                     /locus_tag="IWO50_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0359017.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKLQL"
     gene            10643..11005
                     /locus_tag="IWO50_001895"
     CDS             10643..11005
                     /locus_tag="IWO50_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359018.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11008..11190
                     /locus_tag="IWO50_001896"
     CDS             11008..11190
                     /locus_tag="IWO50_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359019.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11207..14638
                     /locus_tag="IWO50_001897"
     CDS             11207..14638
                     /locus_tag="IWO50_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0359020.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGDTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14689..15426
                     /locus_tag="IWO50_001898"
     CDS             14689..15426
                     /locus_tag="IWO50_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0359021.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            15436..17727
                     /locus_tag="IWO50_001899"
     CDS             15436..17727
                     /locus_tag="IWO50_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359022.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            17751..19826
                     /locus_tag="IWO50_001900"
     CDS             17751..19826
                     /locus_tag="IWO50_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359023.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSSGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVVTLDPNNKLEIFKAKSQPALVVGKTYTMSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSELYTRNTLTATKGVWQKLTGTVKITAASDNAESWLIMLQNKDA
                     NNSLSGKLRLRHAKVEYGSEATPFQPNLLVEPYNICREYPNENLCNPTVKFPIKSSVE
                     TLYSGRVPEDFVKGETYTVTIGATKPSTQSFGVYLALENHAIFKSVEGLPNTWTATLK
                     IDRLGEKKNAVYIYQSPASSVGACQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNNPK
                     DYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQ
                     TKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGK
                     RSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSD
                     YTFAITYTI"
     gene            19989..20435
                     /locus_tag="IWO50_001901"
     CDS             19989..20435
                     /locus_tag="IWO50_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359024.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLCDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20437..20574
                     /locus_tag="IWO50_001902"
     CDS             20437..20574
                     /locus_tag="IWO50_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0359025.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            20612..20905
                     /locus_tag="IWO50_001903"
     CDS             20612..20905
                     /locus_tag="IWO50_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359026.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            20902..21126
                     /locus_tag="IWO50_001904"
     CDS             20902..21126
                     /locus_tag="IWO50_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0359027.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            21123..>21662
                     /locus_tag="IWO50_001905"
     CDS             21123..>21662
                     /locus_tag="IWO50_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0359028.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLS"
BASE COUNT         7617 a         3280 c         4624 g         6141 t
ORIGIN      
        1 tatttaatca aatctaaaga tggtgattca tattatttct gtgattggtt tgtacgagat
       61 ggcatagtga cgcaagagca aggagaagaa ttactcgctt gggcaacaag acaatcatat
      121 gaaacactat tgagcctata caacggctac gaggttgaga aagagccgtt atgggcaata
      181 aagaatgccg atggaaacta tcttactaaa tgtgctttat ggggaaaaga tggagtaaat
      241 tatagttttg aatgcaatcc atctcatcga ttgcttttca ctgataaagc aacagcggat
      301 gctgcagcat tgttggtgaa tggaacagtg gaagaggtgg tagaaagatg aaactaaaag
      361 acggatttta cgctagcagt cacggtatcg gcggtttaat gctagatatg ccgacaaaga
      421 accctaaaac acgcaagaaa tcaaaattca aagtcggtga catggtccgc tgtgaagcag
      481 aagagttcgt ttatccgttc agaggatacg ttaaaaagat actgtcaaac tcagcaatca
      541 ttcgtattga aaatacaatg aagtgtgatc gttcgactgc aaaacacaag cattatttag
      601 ctgtagcgag attagttgac atggaagtaa tcaagagcaa ataaaaaaag ccggatcgct
      661 ccgactgatg taataaatcc gacaagttta ttatatcaca taaaggagcg gtttgacttg
      721 atgcaattgt tacgagaggt agatttcaaa cagacaagat gtaatgcgag agatgtgctg
      781 aagaactttc ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgccg
      841 attattacgg atatgccgaa ggcaccgaag cacggcaata aggcagagga cgcgatcatt
      901 cagatgatgg atatagaagc ggagagagac gcgattttag cggctttgat ggctcttagt
      961 ctgattagtc gtcagatact ctactacagc ttttgtgtgc cagacagctt ctcaaactac
     1021 agaattagcc gtgaagtggg ttattcagaa agaagtatac aacggatgaa atcggaagct
     1081 ctaatagagt ttgcagaagc atataaacac ggaagaataa ttgcttataa ataatttggc
     1141 ggttttttgg cggaatgatg gcggttttta gctatttacc agtgatatta tgatagtgtc
     1201 gaaagatttg gacaggatcg acaaacaaga tagacagcag cggaaatatt aagagtaagg
     1261 atgtgaattt caactccttc taaattgttc ttattatcta tcatccgttg ctgtctattg
     1321 tttttttaat tattcacacg ataactaaag gtgggtgaag agaaatgatg gcgttaataa
     1381 tttcaatttt tgcgctctgt ctgaatgtct atatgattgg atttaaaaat gggcaaaata
     1441 aaagatagta gtaactaaga aagaaatttt tatattgtca ctgtggcgga aagggtagac
     1501 gctaagcatg tgtgctaggt caatgcttcg gcaactatgc aatgttcgat tcattgccag
     1561 tgacttaagc agcttgcact tggtagacat aaatacctga tgtgtcgtag agttcaaatc
     1621 tctgaatcta ggtgaggtcc gtggtagaac ctattagggc gggctgttgg ttcaattcca
     1681 actttggtgc aagttgctat tacatattag atcactcgtt gagtggtctt tttattttgc
     1741 acaaaggagg taacaacaat gtatagacca caatacttag aacagaagta tgaagtaatc
     1801 actgtgcaaa atggtaacgg tgagatagta cgaaagtata gaagaccaat aaagagcgat
     1861 acatataaac gaaaggaaag gaatgaagtt attccattgt atggcaaaag aatagctaag
     1921 cattaaataa gattgcgaaa ggagacgggg catgaccgag gaattctata gatggctatt
     1981 acagttgata agagaagatc gtttggttaa gttctatcag tctcctaaat ggcgcaggct
     2041 tagagagaaa gcgatgaaac gagatcacta tgaatgccaa gagtgtagaa gactaggtaa
     2101 gtatcataga gtagagaacg ttcatcatat aaaggaagtc aaggatagac ctgacttagc
     2161 tttagattta gataatctta tttgtttatg tgttgaacat cataatgaag ttcatggaag
     2221 atatcttaca gcgttagata aacaagagaa gaagatagaa agctttgcta acttcgacgc
     2281 aagtgaaagg tggtaagtgc atgatcatca atgacaatgg cagagagtat gatacagaaa
     2341 agattgaaga gtattcatct tatacacaag gattaattaa acgtttgata tacgttcgct
     2401 atgtaggtat tagggatctg ttatcagata actgttgtag taaatacaaa gtgaatcaag
     2461 tgagagaatc attgaataaa gataataacg ttgaaagaat aaaaaatatt tttggatatg
     2521 gcattgaaga gattaattat tacattgact tcgctgaagc tttcattccg atggtgagat
     2581 aacccccccc cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg agggggctgt
     2641 caggaaaaga gattttttcg aactttatca tgaaaggagg gctaaaatgt ttaaaaacga
     2701 attgtctcaa aatcgctaca gagaaaaatt acgccgctct ttaataagcc aattggaaag
     2761 tcagaaaaca aatattgagc cattcttaga taatgttgat cgttatatca gtttatggga
     2821 aacggcgata tcactggaag aagatatatc cgagaacggc attagactgg agaatggtaa
     2881 aaagaatgaa tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga
     2941 taaacttgcc attactcctg aattggtagg tgaagcaaat gaatcaattc ctgagttata
     3001 agcatattga aaattggttc aaagctatag aagaaggcac tatcaaggta tgcaaagagc
     3061 aattattgct aaaaaattat ctagaagaaa gagtctttac tagagaagat atttacttcg
     3121 ataagcagat ggtagaggat tcaatcaata taccagcaca atactttcca ttcgaattaa
     3181 ttccgtggga aaaatttcta caatgtttta tttatggtgt tcgatggaaa aaagataaaa
     3241 cactagtgtt caatagatat ctttcattaa tgggacgtgg taatggtaaa actggttttg
     3301 cttcttggaa caacttcttt ctactaaccg ctaaacacgg tattaaaaat tatgatattg
     3361 atatctatgc caataatgaa agccaagcaa agactagttt tgatgatgta tttaaagtaa
     3421 ttaaagatca tcctgattta gataaaaaag tatttaaagc tacgaaggaa gttattcaaa
     3481 atatcgctac aaacagcaaa cttcgttata acacggcaaa tgctagaaca aaagatggga
     3541 agcgaccagg tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa
     3601 atgtggctac ttctggtggt ggtaaaattc gagattatag agaattttat gatacaacta
     3661 atggtcatgt tcgtggtggt ccgcttgatg acattataga agaatcaaaa atgattcttt
     3721 ctggagaact tggaattgac aaggatggag cagaattttc tagtttgttt ccatttattt
     3781 gtcgcttgga taacgataat gaagttgatg atcccgacat gtgggaaaaa gcttgtccaa
     3841 ctattaatta caatgcagat ttaaaacgga aaatgtttca agaatactct caaatgcaac
     3901 gtaatgctgg tttaagactt acgttcatga ccaaacgaat gaacagacct atggaagata
     3961 cacgatttgc tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa gaatttcctg
     4021 aaaaaatgga tgaagtgata ggaacagtcg attttgctga tagacgagat tttgccagcg
     4081 ttgggttgct aggaaaatac gataaagatg tttattttac acaacatact tttatccacg
     4141 aatcagccct tcgattacaa aacatcaaac gagaggttat agatatttct atagatcaag
     4201 gaaaatcaca gatcgttcat ggaaaaaata tagaagctga ttatattgta ggttggtttc
     4261 ttgaaatgag taataaatat tatattaaaa aaatcgctat ggatatgtac cgtgcaaaaa
     4321 tattgaagcc cgctttagaa gaagcaggtt ttactgtgga aattgttcga agcggatctg
     4381 ttacacatgg tatgttaaaa gatctggttg atgacctttt tattaatcaa cgtttatttt
     4441 ttggtgacga tgcgattatg cgttggtatt gcatgaatgt atatgaagag catatttcta
     4501 atggaaatat acgctatgaa aaaatagaac ctgaaactag aaaaacggat ggcttttttt
     4561 cattccttca tggtttgaat tttttagatg atatttatga ttctgctcct gtaacagtca
     4621 caaatagctc agtagaaaat acaggaactg gatttactcc tctagtattc taacttgaaa
     4681 ggaggtgaga aagtggggat ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg
     4741 gttcctttag aagaatactt ttgtaaattg caagttgatt ttgtgtatcg aaaatttgcg
     4801 attgaaactt gtattgattt gattgcaaat gcgatgagta aagcggaatt caagtcatat
     4861 gaagatggaa aaaataaaaa gaatgatctt tactataggc tgaatgtagc tcctaataag
     4921 aaaaataatg caacagaatt tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa
     4981 gtattgatcg tttctccgtc taataattct agcgaaatat ttattgcgga tagttgggat
     5041 gtcacagaat atgcattgaa agatgatgtg ttttctcaag tgcaaattaa caacatagtc
     5101 cttgatagag aatttctaga aagtgatgtt atctatataa aatacgcaga tcaacaaatt
     5161 aggcaactag tcgatgcgta ttatcaagcg tatgggaaac tcatttctag tgccatgaat
     5221 gtttacaagc gctctaacgc tcgtagatac gtactgaaag ggaatttatt ccgaccgcaa
     5281 gacaatacaa cacaagatca aatcaataaa atgatgacat cacaatttaa ggcttttatg
     5341 gaagctgata atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt agaagatttc
     5401 agcggaaact tccaaagcaa ttcaagagat ataaaaaact taatagacga catctttgag
     5461 atgacagcag cagcgtttca cgttccgaaa aacctactaa agggagacat gagtgggtta
     5521 tcggatcaag tggacgcttt tttaatgttc gaaatcatac cgattgctga acttattcag
     5581 gatgcgttta acgctagtct ctatgaagta gaagaatact tgtcagggaa ttttgtacgt
     5641 gtggatacaa ctatgatcaa gattactagc ttcaaagatt tggttgacgc tattgatgta
     5701 ggcattagaa atggagtatt tacaatcaac gaaggaagag aacgcgttgg aaatgatcgc
     5761 tctgataagg cgatggcaga tgaaatattt ataactaaaa acaatcaaca agtatcgaaa
     5821 ggaggtgagg cgaatgacga caatgaaaac atttctagca gtaaagaatg aaggcgcagt
     5881 accgcaaatt tttattcagg gatttattgg ttctagttgg ttctttgaag ggaatactga
     5941 caagggaatc aaaaatattt tggatagtct aggtgatcaa gaagaaattg aagtagtaat
     6001 taattcaaac ggtggagacg tatttcaagg gattgctatt gggaacttac ttaagtcaaa
     6061 taaagcaaaa gttaacgttg tgattaacgg cttagccgct agtgctgctt caattatcgc
     6121 aatggctggc gatactataa aaatctacaa caatgcacaa ttgatgattc accgcgcttc
     6181 cacatacgga gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac tggaatcaat
     6241 tgataaatcg gtaaaggctt catataaaac acgattcaat ggcacagatg aagcattgca
     6301 agaacttctt gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt atggattggt
     6361 cgatgaaatt atcgatgcag aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt
     6421 tgaagaaatt ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg catttacagc
     6481 agcattaaat aaaacatttg gacaaggaga tgtaaaataa tgacagttaa aaatttaaaa
     6541 ggtgtaacag ctgcaagcga ccaattgatg aaagctttta aagatggtaa cgaagaatct
     6601 tttagcgcag ctatggtaag cttatctaag gaaattcagg ataaaatttt agaagaagca
     6661 acagcaaaaa atcaagatca attagtatta atgaaccgtg gtcagcgtgt attaactaca
     6721 caagaaacaa aattctataa cgaagtagtg aaaaacgaag gttttgcagg ggtcgaagaa
     6781 ttagtgccag ctactgtatt tgaacgtgta tttgaagatt tagaacaatc tcatccacta
     6841 ttgcaaaaaa ttacttttgt taacacaact ggtgtaacag aatggattgt gtcacgtgga
     6901 gtcaatccag catggtgggg taaactttgc gaagctgtta aaaaagtttt agataatggc
     6961 tttgatgtaa ttaacatgaa gcagttcaag ctatcaggtt atattcctgt atgtaaggca
     7021 atgcttgatt taggtccagt atggttagat cgttatgtcc gtactgtttt agtagaatca
     7081 ttgagaattg cattagaaca agcaattgtt gatggtactg gtaaagatat gccagtcgga
     7141 atgatgcgtg acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa agcagaacct
     7201 attacagctt tagatgctgt aactatgggc ggtttgatgg cgcgactatc aaaattcaat
     7261 atcgaaggtg tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt
     7321 gtgaatccaa ctgattactg gtctaaagta ttcccagcta agactgtact aactgctaat
     7381 ggagaatacg tacaagtatt gccagtacca gtttcagatt tgcagtcaac ggctgtgcca
     7441 gaaggaaaag cagttattgg ggtagcctca gattacttca tgggtgtagg atctacacta
     7501 aaaattgaag cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa
     7561 caatatgcaa acggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct
     7621 ttgggaacta ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag
     7681 gtgatcagaa tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt
     7741 gaagtttgtt taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc
     7801 agacacgatg atcagattcc tatttttttt gagaaggaat atggtgttga agttcatgtg
     7861 tacgatgatc tgcgggacga caaagagtat attccttcga ttaaaccata tttatggtgg
     7921 aaatatttag aagaagatca ttcgcgtgag gacgaccgat atttctatat cgattcggat
     7981 gtcattttca ataaaagaat taatttgcgc aaattgcctt ctaaagatga tgtttggtat
     8041 tgtagcgact gctgtagtta tctaagtctt gattatatta gaagctgtga aaacggagaa
     8101 aatattctaa aagatatggc aaacattgta aatgttacag tagaatcttt ggaaactata
     8161 aacactaatt caggaggcgc acagtgggtt attaaccgcc ctaaagcgaa ttattggaaa
     8221 aaggtttatc tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata
     8281 caaatctgga cagccgagat gtgggcacag ctttggaaca tgatgtattt caatattggt
     8341 cctaaagttc acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa
     8401 gaagtaaaaa tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa
     8461 gggagatacg tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa
     8521 tgctcttacg catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga
     8581 ctgaagaatt gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg
     8641 agtcaattaa aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa
     8701 cactttcttt ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg
     8761 actggaatcg tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca
     8821 ttcaacatta tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga
     8881 ctcgaagaaa cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat
     8941 gaactaggaa aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat
     9001 ctttccatga gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag
     9061 aaagtaaaga ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc
     9121 gtaatcgata gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat
     9181 ttttatcttg aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt
     9241 aaataagcaa atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt
     9301 tcaagactcc gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac
     9361 aggagaaata gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac
     9421 tttctattca gaaaatagag aagatttaac aggagactca ctagatatta tttcattgat
     9481 tcaaaatcgt tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca
     9541 agatcgctat atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca
     9601 tggctaaaaa tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg
     9661 aacgctattc aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg
     9721 ctattgcagt ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag
     9781 ggcaccaaca cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca
     9841 tcattagacc tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt
     9901 ctaaaagaaa tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa
     9961 ttacagaact tctgattcgt caattcgata aattaaataa atagggggaa caacaatggc
    10021 taaaacaaaa actgtagtaa caacgttcga taacgtaagt atcaaacgaa ttgcttttaa
    10081 ttttaagaac gcaggaaatg caatcgcaac gaattgtaac ggacaattag atggcgaaac
    10141 agaaatgcaa acggtggtta aaaaatgtgg agcgacagaa gtaaaatcaa aatctaaacc
    10201 aatcaatatg acggtaacaa ttactgcaca tgtaccgatg gaagtttatc gacgtttcaa
    10261 tgggttgaaa caagatggac gtattaaacc aggcatttac tcttacggtc ctgattccgt
    10321 aggcgaagat ttctcacttg ctgcagagat cgtggatgac ttcgaagaaa atagaaagtt
    10381 agttggtatg ttagcatgca cttcgaatac aggattaaca ttctctattg aaaatggtgc
    10441 ggatgaagta gctgcgttag aactagaaac aaaagttatg caagatgaat ttggtaaatt
    10501 ttatcatgaa gcaattgttg cagaacttga agaagactta acagatcaat ggatgacgaa
    10561 tctatctgct gatgtgatta aaaaacttca gttgtgacaa ctacggccac tcaatcacag
    10621 taaaaaaaac ggaggtagcg aaatgaacga agattactca aaaattgaac taaacgatgg
    10681 aacaattttg aatttagaac ctaaactgaa tatcaagaaa ttattgatga tcaatagaga
    10741 ttttaacaca gacgagtttg caaaaatgac tgtgggaaaa ggatccatgg atatttctgt
    10801 tattcaaggt gcaaaggctg tgtatattgc ttaccgccaa gcgaacatga ctgattatat
    10861 ttcattcgat gaatttattg ataaatggga ttttgatatg gctactgcca gctatattta
    10921 tcaattgatg atgttcaaac aagcacgaga tgcctatcaa aaagaattcg aaaaagcaaa
    10981 taaggaaaaa aagcttcaaa agtaaaaatg ccaaagctct tagttgaaac gtgggtcgat
    11041 gtctattcga tgttgaccga cgttttttct atgccttcag atttagtttt aagcgatatc
    11101 tgtttagatg acattttaca aatggcttac aacaagagcg cttatgaagg atggaagaac
    11161 tacgcaataa atcaatccca aaaaaattaa agaaaggagg taaaaaatgg ctaaaaagag
    11221 aacagaagca gaagtaacat tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga
    11281 aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag ctcaattaca
    11341 acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag agaagcagta
    11401 tgaattacaa agtcaaaaag ttgaagtaac tagccaacgt ttagccaatg ccaaaaaata
    11461 ttatggagaa aattccaccg aagttcagaa acttgaaaga gaactgatta atcaacaaac
    11521 agcgcaacaa cgtttgtcaa acgaaattga taaaacgagt aatgcactag ctcaagcaaa
    11581 aggcgaaata cagacgtacg agtctacaat gcaacagttg gatagtgaac aaaaaaatgt
    11641 tcaagctagt gcttctctga ttgaatccga atacaaaaaa tggcaagcaa ctgctggtca
    11701 atcagcttct gaagccgaga aattagcgaa agcccaagaa tatgtttctc aacaatctga
    11761 aaatgcggag aaaacgatag atatcctaag acgacagtta gaagctacac agtctgaatt
    11821 tggcgctaca tccacagaag caatgcagat ggaagcgaag cttaatgatg ctgaacgtga
    11881 atttgaagag ttaggacaag ctgctaaaaa tgtagataca actaacttgg acgatatcgg
    11941 aagcaaaata gatatgaata atttaatgga agcttctgac gttttaagcg acattggcga
    12001 taagcttaca gaattaggga aacaagcagt ggactctgct aatagtgtag gtagttccca
    12061 gagtaagata caagctaatt ttggtttgtc taaacaagag gctgaagaat taacgaatgt
    12121 agccagagac atttattata aaggttttgg agaatcgtta gatcagtcca cagatgcatt
    12181 gattttggta aagcgtaatt taggagattt aaataatcaa gatttacaaa atatcacgga
    12241 acaagctatg gtcctagaaa acaccatggg cgctgatatg gatgaaacgt tacgtggtgt
    12301 aaatggctta atggtcaatt tcggcttgag tgctcaagat gcaatggatt taatggtttc
    12361 gggtactcaa aacggtttag ataaaacgca cgaattaggc gacaatatgg cagaatatag
    12421 ccaattatgg agtcaaatgg gatattcagc tgatgaaacg ttcggaatgc ttcaaaatgg
    12481 tttagatgcg ggtgcttata accttgataa agtcaatgac ttagttaagg aaatgggaat
    12541 atcgttaaca gatggtcgat ttgagcaaaa catggatatg tttagtgaaa gtactagaaa
    12601 agcttttgaa gagtggaaaa atggcggagg aacacaaaaa gacgttatta attccatgat
    12661 tcaagatttt agcaatatgg atggtcaata cgaccaatta aataaagctt cgacaatttg
    12721 gtctgcgctt ggcgaagata atgcgatgaa agttgtccaa tctttaactg atgttaacca
    12781 tacatttgat gatgttagtg gatctgcaca aaaaatgaat gaagattcta ctactccgtt
    12841 gcaagagtta aacgggaaaa tagctgaatt aaaggattca ttagctccta taggcaatac
    12901 aatcatagat gcactcgaac cagtaattga ttttctagga aagatggctg atgcgtttaa
    12961 taatcttcca caaccagtac aggattatgc cgtagcgatt ggcggattga ctgctgcatt
    13021 tactttatta atgccaataa tagtcggctt catggctcta ggtggtccta ctacattaat
    13081 aataggagca gttattactg ttattgctgg agttatagca attataaaaa actggggcgc
    13141 aattactgac tggtttaagg gaatatggag taaatttact gattggttgg gtgatacttg
    13201 ggaaagtata aaagaaggtg cctcatcagt ttgggatgga gttaaagaaa cctggtctgg
    13261 atttgtagat tgggttcaag atatttggca aggagtttct gattggtttg gagagctatg
    13321 gagcggatta gttgaaggag cttccaacat ctggcaagga gtccaagaga cttggcaagc
    13381 attcgttgat tgggtttcaa atatttggaa cggagtcaaa gaagtatggt cgattatttg
    13441 ggcagacatt gtaggaattg ttcaaatacc atggacatta ataacgtcat tgattcaagc
    13501 tggcattaat attatcgtgg gtatttttga tgtagctgga cagttattag gcgcagcttg
    13561 gcaagctgtt tggacgccta tttctgattt ccttaaaaat acttgggata ctatgacaca
    13621 atggataagc atcgcttgga atggaattgt aactacattc catactatat ttgatccagt
    13681 agtggcatgg tggaatggta tatggacagc tattagtact acggcttcaa atatttggaa
    13741 ttcaattagt gcaacagctt ctagtatttg gaacagtatc aagaatacaa tcactagctt
    13801 ggtacaagca gctgctacag taattcaaaa tatttggtca actgtatcta gttggttagg
    13861 tggaatttgg aattcaatca gctctacagc atcaaatatc tggaatagtg tgactagtag
    13921 tataagcaat gctataaacg cagctaaaag tgccattcaa agtgtttgga atagtatatc
    13981 ttcgtggatt agcggaattt ggaacggtat caaaaatact gctttgaatc tttggaatgg
    14041 aattacaagc actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg
    14101 gtccaatcta acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa
    14161 catatgggaa ggcatcaaaa agactgttag cgctccaatt gattggatta gagacaaaat
    14221 cagtggcatt tttgataatt tgaatatttc tataccacat attccgttac cggaatttat
    14281 catggaaggc agctttaacc cgctaaaagg tcaaatcccc catttgcgtg taaaatggca
    14341 tgctaaagga ggtatcttta ctaaaccaac tttactaggt ggaatgaacg gtgtcggtga
    14401 agcaggacct gaagcagttt tacctttgaa aagatctgtt ttgcaagaaa ttggtgatcg
    14461 tatcttgagt agcacatcag tttcatctag ggcacaaacg attcaacctg tgaataacta
    14521 cgaattcaat ttcacaattg atggtaacgc agatgaggtt actatgaagc aaacaactca
    14581 acaaatcatt gatagcatta caaaaattca aaatgataat gcttcggcat ggcgttaaac
    14641 aggagagtat ttctcctgtt tttttagtat taaaaaggat gtgaaaaaat gactgattgt
    14701 atacattcta taatcgatgg atttcctgat tatttgcata aattggcttt agctgaaaga
    14761 ccaaccatac cttctccaaa aagacagaga gttgaaactt ctgttttagg taggttaggt
    14821 ggcttagtac aagattactc gtttgaagat atgtcgttta cattgcacta taactattta
    14881 gaggatgtgg aagaccatca agcgttcaag caatcgtttt atatcatgcg tcattggtta
    14941 aactatgcaa agaaattaga attctctgac gatcccaacg tctattacgt tatccagact
    15001 atcgatattg gggatgcaga aaacgatatt gttgaatggg gagagttcga tgtaaatatt
    15061 actgcgaaac cattcgcaag agttcaagaa gatgtaccta taaccgtaga taaaccacag
    15121 tcatttaact tgctgaataa tagtttagaa gaaagttttc caaagattat catcactcct
    15181 tcagctactt catgccagtt catcttaaat gattatgtgt ttagtttcga aggcttagta
    15241 gcaggaactg atgtagtcat tgatagtgat ttgatgcttt gctacgaaga gcaatcggac
    15301 ggagatattt tagatcggtc caacaaaatg aagaccatgc aatatccgac attgcaagtg
    15361 gatatcaatc attttaattg cactggtttg agcaaaatac aaatttatcg taacgggtta
    15421 aggtaggtga aatagatgat cgataattta ataactattt acgataaaaa tgacgcgaat
    15481 aatttagctg aacatttata tgatacgcaa ggtttaggcg ctttgtcaga ctggttaaca
    15541 gctactgtta gcaataaact aaacggagcc gagatatttc agggtactta ttcaataaac
    15601 ggaactaatg cagatttgat tgtagaagga cgtattattc agtgttatgt agatgaaaat
    15661 cgagcaaaac agcgtctacg gatttattat gcaaaaactt ctgtaatagg caatacgata
    15721 gaagtaaaag ctgaacctat tttcaatgat ataagaaaat cggtgttgaa taaatatgac
    15781 agcggaacag aaaagatcac tgctactcag gcatggcaaa acgcaaaagt tttagcgaaa
    15841 ccagctatcc cttcgcagtt ttctttctcg tcattagtag atacgcttgc taatgtgaag
    15901 atagaaaagg cgaatttttt agaattcttt ggtggaaaag agggatctat tctagatcga
    15961 tttcatggtg agtttctaaa agataataac acattacgtc atgaaaaaag tctaggtacg
    16021 gatcataaaa tcaaagcgat ttatactaaa aacttaactg gtcttgactt agagattgat
    16081 gctcaaagtg ttttagttgg agtttatcca ttcattagca gctcttcaga aggagaagac
    16141 gagatcactc taccagaaga agttattttc acggattacg tggatgatta tcctgctgga
    16201 tatgtttctt ttgttgattt taaagacaaa gcgactgatg tagccacatt aagggaagct
    16261 gctaaagact ggttgaaaac aaacatagat aaacaaaaac cacaagtgag tggttcgatt
    16321 gaattagtac cattgaggca tcaaagaggc tatgaaaaat ttgttgatct agaaaaagtt
    16381 tcgatgggtg acggagtaga tgtgtatcat ccacagttaa aagtgaatat gtcagcgaga
    16441 attgtggaat atacgtttaa tgttctaacc aattcatacg ataaattagt tgtaggaaac
    16501 gtcaaaacaa acttcttaga aaacacagag aataatgtca gcaatttgat taatgatgcc
    16561 attgatcaat tgaaaaacgg tggcgaaatc agtgatttac tcaatgatat tgtagatcat
    16621 caaactgata tgattactgg tcaaaatggt ggttatgttt tattagatcc taaagaagcg
    16681 cctagtcgta ttttgattat ggacacacca gataagaata ccgcaaggaa tgttttacaa
    16741 atcaacaatg ctggtattgg tttttctaaa actggcatta atggaacata tgaaacggca
    16801 tggacgttag atggcggatt caatgcctcg tttattacgg ctggtgaaat agtaggaatt
    16861 actattagag gtactacatt aattagtgat ggtgctgatt atagaacaag tattgctaat
    16921 ggcaaaatga cttggtactc aaaaaaagtt aacaaagata ttatggagct agaagcacgt
    16981 gattatgtaa gtgctgatgc cggtattgta tcatacacca tgaaaactgg tggtggtttc
    17041 atgattagaa atccacaggg taacttggtt tttagtacgt gggataatgg taataacaga
    17101 ccgtttctat cttttggtgc gcccaatttc aggtatagca atgctagtta tgtaacttct
    17161 ggcgacggta gttctttaag cattaatggt agtgcgggta actcatggga atttaaggta
    17221 gctggtagga ctatgaaatt tactagtgat ggtatgctaa cgttaccagg ttgttttttt
    17281 ggttcatggg aagatgggaa acttgctagg tttgaacaat caacggtaca agtatataaa
    17341 gattttactg ttagaggtac taaaaactca actgtaccaa cagaacatta tggacaacga
    17401 ctattgaacg cttatgaaac tccagaatat tatttcgctg attatgggga agccgttaca
    17461 ggtgacaatg gtaaagttcg tgttgatatt gaccccatgt ttgctgagac agtaaatcta
    17521 agtcggtata tgacacatgt gacacctaca gaactagttt tgtgtgctgt tactcatgaa
    17581 gatgttgacc atttcatcat tgaaactagt aagccaaacg tattggttag atggaattta
    17641 gtggcacatc gactagggta tgaagatgtt agattaaaag aggatacagc atatgatagc
    17701 acagtgcttg accaaaaacg tttttaaaac gaagacaagg aggtatataa atggctagca
    17761 gtttatataa tttggcttta gatttcagca aagaattaaa ctacaccaaa gctattatgg
    17821 ctcgtcaagg tgataaaggg atcacggtga ctgttaaacc atttttaaat ggcctacaga
    17881 tggatacgag tagcggaaca tttactttaa aaggaacaac gccatctaat cgttacgtag
    17941 atagtgtggc aactagtgta actagtgaag aagtcacatt ttctcttgat ggcacattta
    18001 tgagtgaagc aggatattat aaacactgct acgtagaata tagaaaagac gatcaaattt
    18061 taacaacgca agatatcatt tttttctcac taggagtgtc tgacatttcg caaggccaag
    18121 ccgatgaata cgtttcgcaa ttggaagagt tgattcgaaa gtataacgaa acttttgatg
    18181 cttttatggc tgaaattaaa ggtagagtgg atagcttaaa tcaacagatt actgatttaa
    18241 ctggtcaagc taaaacgcta caagacaagt tagatgctct gaaagaagaa atttctaagt
    18301 taggtaactt acaagtgatg tacagtaaca gcatcgactt tgggggctac gattatagcg
    18361 ggaatccgaa tttaatggct aatataaacg ctgacagctt ctcgcaaggt agtggtgcct
    18421 tatctgttgt agatgatggt gacgaggtag tagtcacgct tgacccaaac aataaattag
    18481 agatattcaa agcgaaaagt caaccagctc tggtagtagg aaaaacatat accatgagcg
    18541 tagaaattat gctagaaggc gatttcactg gagaccctag caagataggc ctaagatata
    18601 ttaaaatgcc taactgggta tcagagctat atacgcgtaa tacattaact gctactaagg
    18661 gtgtatggca aaaactaact ggcaccgtta aaattactgc tgcgagtgat aacgctgaaa
    18721 gctggcttat aatgctacaa aataaagacg ctaataacag cctatccggc aaactacgtt
    18781 tgagacacgc taaagttgaa tacggctcag aagcgacacc gttccaacca aacctattgg
    18841 tagaacctta taacatatgc cgcgaatatc ctaatgagaa tttgtgcaac cctacagtga
    18901 agtttcctat aaaatcaagt gttgaaacgt tatactcagg cagagtacct gaagatttcg
    18961 ttaaaggaga gacgtacact gtgaccatag gcgcaacaaa accttcaacc caatctttcg
    19021 gggtatattt agcgttagaa aatcacgcca tattcaaatc ggttgaaggt ctgcctaata
    19081 catggacggc taccctaaaa atagaccgtt taggagaaaa gaaaaacgct gtgtacattt
    19141 accaatctcc tgcgtcaagc gttggcgcgt gccagataga ctggctcaag atcgaaaaag
    19201 gcgacacacg aaccccgaat attgagcaat ataaataccg aggaatcggc atgcgtgatt
    19261 caaacaatcc aaaagattat gtttgggatc tagcaccaga atatgtcgaa gataatttgg
    19321 ccacagatat taaaatttct gaaattactg gtaaagcaaa caattatacc gatgggaaag
    19381 tatcggagat taattcgcag ttgactgctt caattaatga agtagacacc acagctaagg
    19441 atgctcaaac aaaagcgaat gctaatgcga ctgctataga tgaattagac aataagatcg
    19501 atgaacgcat taatgataca gctactacca cattaacagt tacaaacggg aataccggat
    19561 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
    19621 aacgcagtgg tggaaatgat tcaacgatac taacaattcc agaaggctat cggccaccaa
    19681 ttagttttga gcaactggtt ggctcgatag accgttctac tttgaacagt gctcagttat
    19741 ctattggtgc agatggagcc attaaatggc gaagaaactc aagttatgga tcggattata
    19801 cctttgcaat tacttacacg atttagaaaa gcgtgaatcg atatgaaggc agcatataga
    19861 ccaattgaac cttacggatt cgagcaaatc attgtgaatg atgaagaaca tttaccggaa
    19921 gaatgcacag aagtcgaacc accgattcca aattggaaac cgaaattcaa ttactgggag
    19981 ggaaataaat gaaaaacatt tggaaatatg gacgtactgg cggagagtac gcaggaaaag
    20041 tattggacga catgcttgta tccgttcctt acacagatca gccaccgctt gaaggaattc
    20101 gtgctgatgg cgaaccgtta acaatcgctg atcaaatgtt tgatcctaaa ttgaatcaat
    20161 ggattatttt agcgaacgca ctagatcaca acgatttaaa caatctcaaa gcaatgtatg
    20221 agtcgttaga aaatgagaac ggcgatttaa aacagatcaa tgccaaactc atgctatgcg
    20281 atgtagcaat taaacaggaa aatactgcat tgaaagaaaa agcggatagt ttagcacaaa
    20341 tcaattcaaa aatgatgctt gcttcgttac aaaatagcaa agacatttca gaaattaaag
    20401 agcaactaaa tccagcttca aagggaggtg agtagtatgt ttagttttag cgatgtgaaa
    20461 atgatgtatg attggggctg tttcactaac gaacaagtaa tggttttcgt tccgttgtgc
    20521 attactgaag aaaaagcaga taaaatcatt agcaaagaag agagcgcatc ttaattgatg
    20581 tgctttttat tttgattcaa ggagttgtca catgattaat ttaggggaat ggggagcgat
    20641 agcaggatca ataaccgcta tcgtttcttt gattttatta gtaataaaac caattactgc
    20701 atctttctcg aagattactg agactctttc aaaagtaagt cacaatttag atttgctgac
    20761 taaagattta gaatcgagca aatcagatcg attgatgatt catgaagaac taaagaaaca
    20821 cgatgaaaga ttagatacac atgcagaaaa attggtagaa cacacgcaac aaattaaaac
    20881 tttatttagg gagagaagaa aatgaataat aaaacgttcg aagtactaaa atggttcgca
    20941 ctggtaatta ttcccgcact agctactttc gtggggttag ttggtaaagc gctcaattgg
    21001 cagtacacag atatctgtgt tgtcatcatt actggttttg gcgcgttttt agggagtgtg
    21061 ttgggtgtat caaatcgaac ctacaaaatg ttctcggctg aaagcgaaga aggaggaaac
    21121 aaatgaaaaa gaaaattact attactgcga tgagcctgtt aacggctctt tttttattac
    21181 ccattaatac gtttgcttat actattaatg acgagtataa tttagcgccg aatcaaggag
    21241 actccagatt agcaattcct aacaaaatta ttttgcacga aactggaata gatgcaccag
    21301 caagaaacgt agccgccaac atgaaaaata attataacgg aagcaattct tatactacag
    21361 atgttattgg tgacggtggg attgtttacc gtgtgggtga gcaaggatat gtttcgtggg
    21421 gagctggtaa cgctaatcct tatgcgcctg tacagattga attacagcgc acatatgata
    21481 aagcattgtt tgaaaaaaac tatcgagctt atattgaata tacaagagat agtgcaaaaa
    21541 aatatggaat tccattgact cttgatcaag gaacttcttt atttacaaaa ggaatcattt
    21601 ctcatttgtg ggtgacaaat tatgtttggg ggaaccacac agatccatat ggttacttat
    21661 cg
//
LOCUS       DACEDM010000055        21450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEDM010000055 DACEDM010000000
VERSION     DACEDM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.055"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..403
                     /locus_tag="IWO50_001906"
     CDS             5..403
                     /locus_tag="IWO50_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0359029.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IWO50_001907"
     CDS             387..1082
                     /locus_tag="IWO50_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0359030.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="IWO50_001908"
     CDS             complement(1666..2553)
                     /locus_tag="IWO50_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0359031.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="IWO50_001909"
     CDS             2738..3577
                     /locus_tag="IWO50_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0359032.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4693
                     /gene="sgrA"
                     /locus_tag="IWO50_001910"
     CDS             3773..4693
                     /gene="sgrA"
                     /locus_tag="IWO50_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0359033.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            4991..5347
                     /locus_tag="IWO50_001911"
     CDS             4991..5347
                     /locus_tag="IWO50_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359034.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5350..6507
                     /locus_tag="IWO50_001912"
     CDS             5350..6507
                     /locus_tag="IWO50_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359035.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6533..6973)
                     /locus_tag="IWO50_001913"
     CDS             complement(6533..6973)
                     /locus_tag="IWO50_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0359036.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7133..7639
                     /locus_tag="IWO50_001914"
     CDS             7133..7639
                     /locus_tag="IWO50_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0359037.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7807..9720
                     /locus_tag="IWO50_001915"
     CDS             7807..9720
                     /locus_tag="IWO50_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0359038.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9725..10726
                     /locus_tag="IWO50_001916"
     CDS             9725..10726
                     /locus_tag="IWO50_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0359039.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10928..11134)
                     /locus_tag="IWO50_001917"
     CDS             complement(10928..11134)
                     /locus_tag="IWO50_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0359040.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11780..12628)
                     /locus_tag="IWO50_001918"
     CDS             complement(11780..12628)
                     /locus_tag="IWO50_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0359041.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12792..13694
                     /gene="gnd"
                     /locus_tag="IWO50_001919"
     CDS             12792..13694
                     /gene="gnd"
                     /locus_tag="IWO50_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0359042.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13696..14697
                     /locus_tag="IWO50_001920"
     CDS             13696..14697
                     /locus_tag="IWO50_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0359043.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14694..14978
                     /locus_tag="IWO50_001921"
     CDS             14694..14978
                     /locus_tag="IWO50_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0359044.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15011..15808
                     /locus_tag="IWO50_001922"
     CDS             15011..15808
                     /locus_tag="IWO50_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0359045.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15822..16655
                     /locus_tag="IWO50_001923"
     CDS             15822..16655
                     /locus_tag="IWO50_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0359046.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16673..17530
                     /locus_tag="IWO50_001924"
     CDS             16673..17530
                     /locus_tag="IWO50_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0359047.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17552..18061
                     /locus_tag="IWO50_001925"
     CDS             17552..18061
                     /locus_tag="IWO50_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0359048.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18098..18499
                     /locus_tag="IWO50_001926"
     CDS             18098..18499
                     /locus_tag="IWO50_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359049.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18611..18964
                     /locus_tag="IWO50_001927"
     CDS             18611..18964
                     /locus_tag="IWO50_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359050.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            18998..19441
                     /locus_tag="IWO50_001928"
     CDS             18998..19441
                     /locus_tag="IWO50_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0359051.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19458..20543
                     /gene="uxuA"
                     /locus_tag="IWO50_001929"
     CDS             19458..20543
                     /gene="uxuA"
                     /locus_tag="IWO50_001929"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0359052.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20536..21249
                     /locus_tag="IWO50_001930"
     CDS             20536..21249
                     /locus_tag="IWO50_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0359053.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7379 a         3293 c         4472 g         6306 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcatga gtagtgagag
     4381 tagcacagga agcacgagca gcgaaagcag cacaggaaaa gtaaataatg aacttgattt
     4441 ggtacctatc ataccaacta aggaacaaaa gagaaatgca gggagcaacc taaaaagtgt
     4501 gctaaaacag cctgattttg tttctcgtgg aattgatcat caagaaaaag cgaaagaaac
     4561 agataataga aaaagcgatt taccagaaac tggtagtaga accttgaaca agctaattac
     4621 atggatgggt gtgttactca ttttgattgt aggagcaagc tattttcgtt ctctcttcca
     4681 tcgagtgaaa taaagaataa aagcaacaga tctaaaattg aataaaacga tagatttttg
     4741 agtgaatatt tcattcgaga gtgctgtatt gcctggcgaa gttgtagctt tgccagatct
     4801 atttttgttt actaacatat ttgagaaaca ggaaataaca ggaacacaaa tggaaaataa
     4861 gagcaagaat aattgacaat gcacactgcg catggtagta tctctgaaaa atcagtaacg
     4921 aatctatttg tacgttattc atgtagctgt cggaataagt ctaaataata gataaaaagg
     4981 agtatgtact ttgtttgaag aatttattga tatcaatgaa aggcaagtat atcaattttt
     5041 gaactattgt tatgaaagag acgagaaatt atatgtggtg aaggatatcg ctttagatct
     5101 aaactacacc ctggctaaga tgaattcggt gattcaacaa gcagaatcgt tttgcgaaag
     5161 gtatccagaa tataaattat cttttctttc agaaaacaag atgataaaag tcgagttttc
     5221 ttctcagttt ttactgagta aagtttattc tatactttta gagggaacga ttggttatat
     5281 tttactggat tcactttaca aaggtactta tcaatcctta gaaaatctta gtcaaaaaat
     5341 tatataagtt tgcgtacgat tcaaagaaaa ttaagagaac ttaatactat tttgaaaaac
     5401 tatcatttat cttgcagctt gaaaaaaggt aatccgatag aaggagaaga ataccgtatc
     5461 cgatactttt ttcacttgat gtattggcag tcattcgacg aaaatcattc tgatatatta
     5521 tcaagtataa aaaataaacg tcaactatta gaaaaatttg cgaaacatgc gccatatacg
     5581 cgtagtatag atagaaagaa atggatcagc ctactaagaa tttcgttgca acgaatgaaa
     5641 caaggccact cgattacgta tgtcccagaa gagatgaaca aatttgtgca tccattgata
     5701 acaaaagaag agtttacgca ttacatacta gagccttttt ttgaagcaaa cttctggtcg
     5761 atgaatcaca tcactccaac agagcaagct tacctttatt ttatgtttgg tgtaatgaac
     5821 acctatttgg aagaagattt gttggatact ccagataaag tacaagaaag ttggggctgg
     5881 attgaatcgt ctattgtaga aaattttgaa aaaaacttca agatttgttt tacaacttca
     5941 gaaaaaaatt atctatatgt caatttggca atgatccatt tgtattcaag ggtttttggg
     6001 acaaaaaaga aggtggatgc ttttggaaaa tcagcagaag acagagattt tcagcatata
     6061 tttccagtga tgtatccaat catgcaacag ttttttagtg gggtagctcg cagaattcca
     6121 gagcttaata cttattatga aaagaacaga cggttgattt ttcaatactg tatgttagtc
     6181 agggatattt ttataaaaca cgaacaacct gtcatattct atattcaatc aaagtatggt
     6241 aaaacacagg aattatgggc aaagaaaagg atattatcta tgactgaacg cccaataaaa
     6301 tatagtgcat ccgctgatca gttacctgat attgtcatta gcgattatcc tatcgacaaa
     6361 aaggcatatg atcaaacgaa tacacagata ttttactgga atggacaacc tacaaaaaat
     6421 gattgggcgc gtctgaaagc tgaaatcata aaaatcagga atgagaaaaa tagatccata
     6481 ttggaaaaat tggatttcgt taattaagaa aagcagattg tttatgccaa aattaagaca
     6541 ttgcaaaaag attattttct ttaaaatcga caagatgacc agaaagcaag agttcgttga
     6601 gctcttgctt tcttttagtt ttagaaagga acatcctgat tttatcttcg ctaaatccag
     6661 tcaaaagttt tgcagattgg tcgtagataa tcggtttttt caataaagaa ggggatgtac
     6721 gaatctcaat atatagttca ctcaaactga gttcatctat tctgtgaaca agatttttat
     6781 aactaataga atgagtagag agcatttcgg tcacgccgtt gtttgtcaat tttagtaagt
     6841 ggataaattc atttttggtt agtggttgac gaattaagtt ctgttcgata aacggtatat
     6901 catggacgat aagccaacgt tttgcgcgtc ttgaagacga gcagccagaa cctgtataca
     6961 atttaatcat catacattcc tccaatagct aagtatttat gaaataataa catgaagaaa
     7021 tagtcgaaaa gtgccattta gaggtgtaaa ataagaaaat gttgtcacgt ataatggaac
     7081 tagctagata agaaatacta ttaaatcaag aaaaaagaaa gaaggcacaa gtatgtcaaa
     7141 cacagccgac gcaatagtag tgatcgattt acaaaaaggg gtatgttttg attcttcgcc
     7201 gatacacgaa ttagaaacag tgacagcatt agtgaataaa agaatcaaaa catacgaaac
     7261 gattgggaaa ccagttattt tcgttcaaca cgaagatgct caattagtgc cacaaacaaa
     7321 accatgggag ctattgtcgg aattatctat tcctgagcac agttattttg tgagaaaaac
     7381 ccatgcgaat tcattttata agactaattt gagtgtttta ttaactgaat tggactgtca
     7441 ttcgatcgaa ttatgtgggg cacaagcaga gtattgtata gatacgactg tgaagtttgc
     7501 tcatggatta ggataccagc ttatgatgtg tagaggagca acgacaactt atgataatcc
     7561 ttttatgaaa gcaaaggaaa caatcgcttt ttacgagaaa atttgggata aacgatttct
     7621 gacatttttg gagcaataag ttgtttcttc gtttaaaaaa agcagaatgg atactggttt
     7681 atccattctg ctttattacg taaaatgaga aaataatcct gttctatttt tatgggataa
     7741 accatttatt ttttgtataa aaaatgtttg cgtttacata taattctcct agaaacataa
     7801 aaaggagtga atagcttgag aacaaaacaa gtattaaagg gagcgttggt catcttattt
     7861 gctgtaagta caagcgtccc gcaatggaca gtatatggag agagcatttc ttccgaatcg
     7921 gaatctcggg aggtagaaac cacagaaact actgatgctg gaaatgaaac gaaatcgagt
     7981 acttcacaaa cagaaacaaa tactgaaaca acggttgaga catctgttac tagcacagat
     8041 caagaaacga ccgctaccac agataaccaa caaacagaag aagcaacagc tacaaaaatg
     8101 tcaaacgacg aatcctcttc tgctaaagta agtgacaaag agaatgggac agaaaatgat
     8161 gatatcggta aaggtgaaaa tttcaatggt aaagaatatt tgaattactt cggtaaacag
     8221 atgacagaaa tacttaatgg accaacagaa gacatcaagt caaaattaat agagttatta
     8281 gtcactgagc ttgctgttaa tgattatggt gtagataaag agattatcaa acatctgatt
     8341 gctacttatc tattgtacga tatcgctttt gcaaaaaaca gtgtgttgat gtcgatgctt
     8401 ggaggtacag atgatgggac aacgcctaca gaaaacacag gcttctatcc aaatacaaaa
     8461 caacttcatc aaactgtact aaacaaacaa gatggcaaaa tgatggatgt atatgcatat
     8521 tatgtcgatc aaggttcaga taaaacagtc atgatccatg gcggatttcg agggaattgg
     8581 aataacggga tcgttacaga agaatacaat gatttttata aggccgggta taatttatta
     8641 tttgttgatt ccagagcgac tggtaatagc ggaggcgatt atgtgaccta tggacaatat
     8701 gagtcagatg acgtccttta ctggataaat caagaagttc gagagcggcc atctcaaaag
     8761 attttacttt atggtggttc gatgggagca gctacgatga tgtctgtatt agcgaaagat
     8821 attcctgtca atgtcaaagg aattattgaa aattgcggat ttgcatcaat tgatgaacaa
     8881 ctacgtttta cgtacagcca gacagtagcc cccgcactac cggatgccat aaaaaatcag
     8941 ttagacatca ttggggatca agagcatgaa gatttattta tgggcctttt gaaacaatac
     9001 tattttgatc aggagatgca tttggataca aaagctgctc tacctacgat tggaatgtca
     9061 gattcattgc ctaaattgat tatccatgga acagcagatg atgtagtacc tgtgtctaac
     9121 gcgcaaaaat tatatgagtt atcaggaggg tataaagatc ttttattagt agaaggagca
     9181 gggcatggaa aggcacagga agttgaccat gctgcctaca cgaaacatgt aacggacttt
     9241 ttgaaagtag tgttccatga tcagataaac gtaaagtatg tggatgaaaa caataaaagt
     9301 ctactcaaag accaggacga aatccagctt tacggagctt atggagaaaa ttatatgaca
     9361 gagcaaaaga cattcgaagg atacgaatta gcaaatgttg aaggaccgac tgaagggatt
     9421 tttaatgaga cgataccaac tattattttc tattacaaaa aaatcccagt agctccacca
     9481 aaaaagcaag atccgacaga gaatgcagac aataagggaa agaatgaacc tcgagaggat
     9541 caatcaagtg atgatacgca accgattcag atgacagtga aagtacaaaa aaagacagat
     9601 gcaaaaaaaa gaatgttgcc gaaaactgga gaaaagaaac attcgcttct catttattca
     9661 ggtggatttt tatctgttag cacgattatc acatggattt ggcgaagaaa gagagggtaa
     9721 atgaatgaaa tggttcaagc gaatagtgat tgcagtacca gttatcatcg tagttatttt
     9781 agtcactgta tttttgatta atcaaatcac accaaagccg attagcttgc ttgttagaaa
     9841 acaatttgac acaagtgaaa aagaacaagt atatgcccga ccagatgatt tccagcaaaa
     9901 aatcgaacaa atcgatataa aaaaagaaat atcctatcct tcaaagtaca aaaataataa
     9961 tatggatatc tacacaccga aagtggaaaa gaaaaaatta ccgattcttt tctggatgca
    10021 tggaggcgca tatgtaggag gggataaaaa tgattgtcgt gattatttgg aatatctatg
    10081 ttcagacacg cagcaaatca tcgtaaatat agattatgaa cgatcaccgg aagcaaaaca
    10141 tcctacacca gttatccaat tgaacgaagc cattcaagca gctaaaaaag agataaaaga
    10201 tcaagcagat tggtcgaata tttcgattgg tggaaattcg gcaggtgcac aaattagtgg
    10261 agaatatttg ttagcacttc aaaatgaaga aattaaaaaa gctgatcatc ttgatccgac
    10321 tttggagaac aagcagatta cgaagtttat ttctttgtct ggtctgttgg atccttctgc
    10381 acttactcaa gtaagtgata aaatctctag ctttctttat gaaaaatgtg gatgggctta
    10441 ctttgataat aaggactttg aacaatcaga gaaggtcaaa agtttagcct tggtcagaca
    10501 tgcggatcgt tggacgcaaa acacattctt aacggatgga aatacaaata cgtttacgaa
    10561 acaaatggaa gaagtaactt ctgtatttga gaaagcgggg gtgaaagtca caaaagtaga
    10621 ttatgcggag aaaaatgctg ttttgaacca tgaatatcag tttgatttca gcaataaaca
    10681 agcgcaagaa acatatcaaa agttagtaat gtttttcaag gagtaaagtc ttatgatgca
    10741 aaagaggttg taatctgtca cttagctcca agtcacaagg agcaatttca ataaacaagc
    10801 ttttcatggt ttatgaatat tgtaacttgt gactgaggag ttagagatgg caaggggcac
    10861 cttttattcg aaatttggga atatcacacg attattgtca cgatctcttt tacgtaaatt
    10921 ttataagtca gatttgttgt tttgatcgtt taaaaaaagc aacaaatccg aagcctgcgg
    10981 aggcaagaag ggacaaaaag ccgagtggta agcaaaagaa tgctggttcc actaatattc
    11041 cgttgttatt gatataaggc tgggaacaaa gatgaatagc aaataaaatc aatgcactag
    11101 cgaaaaaaag caaagaaata tggatagctt tcataagcag cacctccttt tttataattg
    11161 tatgataaat agattaagaa tctataaatg tgacaagttt gttaggatgt gaataaaatg
    11221 atcatctagc gaacttgaaa caaagatggc taatgataag caattaatga ttcagcaatc
    11281 agataggtac aaagtttacc atcatacgtc tagaccaata atagaaaata tgggaatata
    11341 atagaaaaga tttaggatac ccccaaaaga atttccccaa aaattcgatg tatcctaaac
    11401 ttttttattt tacataacaa tcatatcaac tgtgaaataa aattacaaat gataacctaa
    11461 aatgaggcca aaagccagtg ttatcaagga aatacttaat tcaccaaaat tttttttaat
    11521 tttttagaaa ataagaaaca aaaataataa aaaaaagaaa agataaattt tttactactg
    11581 tatactaact ttgttatcgc atctaacaag taactcgaat aataccaata caaccttctc
    11641 caaagatgcg atgacatacg ttctaaccca ccgactattg atgttctgta ccacccttgc
    11701 agaaccaaaa aagccactac caaatatttg gtagtggctt tttttgtttt atggattttt
    11761 cgctttttct gttatggtgc tattctttga tttcagaaat agtatgtcga actttagcga
    11821 tagaacgacg tgattctttt tcgttgtaaa acatcaaaat cacatataga gagtccaaaa
    11881 tgcttagttg agaaatacgg gaagctaaag cttcagatcg gaattctgtt tcttcagaaa
    11941 tagaaataaa aacgacatcg ccaagtttag caagggaaga attagcttgg ctagtaataa
    12001 cgattgtttt tgctccagtt tttcttacgg tttcagcaat atggattgtt tcttttgatt
    12061 gccctgtgtg agaaatcaaa acagcacagt ctttttccgt caataaagaa gcttccatca
    12121 attgaatatg gtaatccgtg ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat
    12181 aagcgtcagt tgctacaatc tctgaaccgc caacaccgaa aaaatagaca cgtttagatt
    12241 cattgataat agtagccgct ttttttagat ccttttgttg taacaacttt tttgtgtcat
    12301 ttaaagttga aatactagac tcgaacacct tttgcgccat agtgagctca gtatctgttt
    12361 tctgaatatt ttcgtggata gaaatagcag atagatcatt ttcttggatc aacatggcca
    12421 ttttaaaatc tttaaatcca ttataaccaa gtttttttgt aaattgaaaa aaagtagagt
    12481 cagcaatgcc cagttcactt gctaaattgc taataggact atgacttact ttgcttggat
    12541 tttctaaaat ataatcggcg atagcctgtt cttttgggct gaagtcgttg tacattgttt
    12601 tgatttgtag tcttatggag acgctcatga gaattcctca ttttcataat aagattatca
    12661 ttttttccgt tctcattaat gttaaagata caaggagagt aggaaaaaat caaacaaaaa
    12721 atatttttgt aaaatctatt tacaaaaaaa tttttttgta taatgagggt ataagaaatg
    12781 gaggcgctat catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt
    12841 taaacttaaa agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta
    12901 gtcaggcaag agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt
    12961 ttgagcagaa aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt
    13021 taagaaaact ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt
    13081 attataaaga ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg
    13141 attgtgggac ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag
    13201 gagataaaga aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag
    13261 gatatctgta tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa
    13321 tcgaatatgg aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt
    13381 atgcttttga cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct
    13441 ggctgatgga aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg
    13501 gaatcgtttc tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg
    13561 ttccagttcc agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata
    13621 gtttttcagc aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg
    13681 aaaagggtga gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga
    13741 agagtctttc cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact
    13801 gttgatcaag agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt
    13861 attgcaagtg ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta
    13921 ttgatttccc gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac
    13981 cagacaatcg tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat
    14041 ataactaatt tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac
    14101 gatcgatggg aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt
    14161 gtgattggtg caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc
    14221 tgtgatgttc tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca
    14281 aaaaaagtag aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat
    14341 ttgacagaag agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat
    14401 gtttatctat ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt
    14461 ctgaaaagcg gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt
    14521 caagatcatc cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac
    14581 acaagggaat gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt
    14641 cagcaccttc ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga
    14701 agaaaatggt gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag
    14761 gtggtatttc aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca
    14821 cccacggaac ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa
    14881 cgattcaatt tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa
    14941 tagctacaat catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact
    15001 gaccgatcta gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac
    15061 tttagctagt aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca
    15121 agcagaaggg aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca
    15181 attagtgatt gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa
    15241 atggtatgaa tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa
    15301 gctagcacaa gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc
    15361 aatccaagat atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat
    15421 ctttgctgtt gctggtttag ttggaggatt aggagataaa tacgcaagaa atgtggcagc
    15481 acatgctatg catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg
    15541 agaaaaagta gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat
    15601 agacgagtta tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg
    15661 attatttccg aaagataaac agataataga taaaatggct gaatttattg attcgaaaga
    15721 gaaagtgcat ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga
    15781 actggaaggt tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag
    15841 tacaaagttt gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag
    15901 gagccacgtt gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca
    15961 tgggggaagt tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg
    16021 ttttttctcc aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac
    16081 cagtcgcatt attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg
    16141 gattgctcgg aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat
    16201 atacagacaa agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt
    16261 atccaacggt tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag
    16321 gggcgccatt gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc
    16381 ttgaagtagt aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg
    16441 gtcgaaaaga ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat
    16501 ctttagatat tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg
    16561 tccagtcgca aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt
    16621 ttgatgacga agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga
    16681 aataatggat aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc
    16741 ctatctgaga tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc
    16801 atcactacta tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt
    16861 aaaagcagca tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg
    16921 tgtgattact ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga
    16981 aaaagagatc ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc
    17041 actagcagga atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat
    17101 cgcggtacct tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt
    17161 tgggttgtat caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg
    17221 taatgctaca ggcctaaagc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt
    17281 tttagggttg tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt
    17341 gaaatttgcg atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc
    17401 agggatatta cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt
    17461 gaaagtcaca caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg
    17521 tattttataa aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga
    17581 tgagcgtttg atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc
    17641 catttttgtt gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa
    17701 tgcaggaagc cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta
    17761 ttgggatgaa tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac
    17821 gatttacgac ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc
    17881 tcaaaaaaat cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga
    17941 tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc
    18001 cactccatct gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata
    18061 aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg
    18121 tgattgggaa tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca
    18181 gcgttttctt tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat
    18241 tgcttacgat acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc
    18301 agagaaagtt atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat
    18361 gtttgattgg aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt
    18421 gttgttcagt ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt
    18481 cgatctgcta tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt
    18541 attgggaaaa gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc
    18601 catattaacg atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt
    18661 tgttgatgaa ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa
    18721 agaggctatt cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg
    18781 aaattatttt tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa
    18841 cgtcaattat atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac
    18901 agatttacga gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg
    18961 ataagtaaaa agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac
    19021 acatggtaag ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag
    19081 ccatattgaa acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt
    19141 caaagaaaaa ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat
    19201 tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa
    19261 agcagccact tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca
    19321 tcagatgctt cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga
    19381 taatttaact gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta
    19441 gaaggaaggc tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga
    19501 tcccattaaa atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga
    19561 ctaaaatgcc tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg
    19621 agagccaagg actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg
    19681 cagggacaga tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt
    19741 ctcgatgtgg ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa
    19801 ctgatttgtt taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg
    19861 tcgatcagat ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca
    19921 aattgccggg ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg
    19981 aaggaatgac cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc
    20041 cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac
    20101 tttttggttt tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag
    20161 tagattctcc atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa
    20221 acgatttagt tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa
    20281 acgtggagtt tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct
    20341 cccttgatat gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac
    20401 gccctgatca tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg
    20461 atcgtgcaat gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac
    20521 cactgggaga gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta
    20581 ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat
    20641 gaaatgcgta aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa
    20701 caaatgggca ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat
    20761 caatataaag aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa
    20821 gaaattttat cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca
    20881 cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc
    20941 atttcagggg atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt
    21001 tcaaaaatga atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc
    21061 gaagcagcaa ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg
    21121 cccgatcata atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa
    21181 gcagactatg aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg
    21241 tatctctaat aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag
    21301 ggaacagatg aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac
    21361 aatttagaac aaacacaaaa cgcatctact gttatctacc agtagatgcg tttaaagaga
    21421 agaacgagaa tcgtccatat gagggtttcc
//
LOCUS       DACEDM010000056        20939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEDM010000056 DACEDM010000000
VERSION     DACEDM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.056"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            165..359
                     /locus_tag="IWO50_001931"
     CDS             165..359
                     /locus_tag="IWO50_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359054.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            628..927
                     /locus_tag="IWO50_001932"
     CDS             628..927
                     /locus_tag="IWO50_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359055.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1024..1458)
                     /locus_tag="IWO50_001933"
     CDS             complement(1024..1458)
                     /locus_tag="IWO50_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0359056.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1690..2400)
                     /locus_tag="IWO50_001934"
     CDS             complement(1690..2400)
                     /locus_tag="IWO50_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0359057.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2390..3250)
                     /locus_tag="IWO50_001935"
     CDS             complement(2390..3250)
                     /locus_tag="IWO50_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0359058.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3255..3893)
                     /locus_tag="IWO50_001936"
     CDS             complement(3255..3893)
                     /locus_tag="IWO50_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0359059.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3908..4207)
                     /locus_tag="IWO50_001937"
     CDS             complement(3908..4207)
                     /locus_tag="IWO50_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0359060.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4250..5734)
                     /locus_tag="IWO50_001938"
     CDS             complement(4250..5734)
                     /locus_tag="IWO50_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0359061.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5754..6215)
                     /locus_tag="IWO50_001939"
     CDS             complement(5754..6215)
                     /locus_tag="IWO50_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0359062.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6233..7300)
                     /gene="ulaG"
                     /locus_tag="IWO50_001940"
     CDS             complement(6233..7300)
                     /gene="ulaG"
                     /locus_tag="IWO50_001940"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0359063.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7518..8288
                     /locus_tag="IWO50_001941"
     CDS             7518..8288
                     /locus_tag="IWO50_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0359064.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8561..9388)
                     /locus_tag="IWO50_001942"
     CDS             complement(8561..9388)
                     /locus_tag="IWO50_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0359065.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9956..11191
                     /locus_tag="IWO50_001943"
     CDS             9956..11191
                     /locus_tag="IWO50_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0359066.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12190..12450)
                     /locus_tag="IWO50_001944"
     CDS             complement(12190..12450)
                     /locus_tag="IWO50_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0359067.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12477..13778)
                     /locus_tag="IWO50_001945"
     CDS             complement(12477..13778)
                     /locus_tag="IWO50_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0359068.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14070..14528)
                     /locus_tag="IWO50_001946"
     CDS             complement(14070..14528)
                     /locus_tag="IWO50_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0359069.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14541..15866)
                     /locus_tag="IWO50_001947"
     CDS             complement(14541..15866)
                     /locus_tag="IWO50_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0359070.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15883..17295)
                     /locus_tag="IWO50_001948"
     CDS             complement(15883..17295)
                     /locus_tag="IWO50_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0359071.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17312..18076)
                     /locus_tag="IWO50_001949"
     CDS             complement(17312..18076)
                     /locus_tag="IWO50_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0359072.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18180..19055)
                     /locus_tag="IWO50_001950"
     CDS             complement(18180..19055)
                     /locus_tag="IWO50_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0359073.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19310..19394)
                     /locus_tag="IWO50_001951"
     tRNA            complement(19310..19394)
                     /locus_tag="IWO50_001951"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19358..19360),aa:Leu,seq:caa)
     gene            complement(19412..19483)
                     /locus_tag="IWO50_001952"
     tRNA            complement(19412..19483)
                     /locus_tag="IWO50_001952"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19449..19451),aa:Gln,seq:ttg)
     gene            complement(19493..19565)
                     /locus_tag="IWO50_001953"
     tRNA            complement(19493..19565)
                     /locus_tag="IWO50_001953"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19530..19532),aa:His,seq:gtg)
     gene            complement(19627..19711)
                     /locus_tag="IWO50_001954"
     tRNA            complement(19627..19711)
                     /locus_tag="IWO50_001954"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19675..19677),aa:Leu,seq:caa)
     gene            complement(19732..19802)
                     /locus_tag="IWO50_001955"
     tRNA            complement(19732..19802)
                     /locus_tag="IWO50_001955"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19768..19770),aa:Cys,seq:gca)
     gene            complement(19848..19919)
                     /locus_tag="IWO50_001956"
     tRNA            complement(19848..19919)
                     /locus_tag="IWO50_001956"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19885..19887),aa:Gln,seq:ttg)
     gene            complement(19931..20003)
                     /locus_tag="IWO50_001957"
     tRNA            complement(19931..20003)
                     /locus_tag="IWO50_001957"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19968..19970),aa:His,seq:gtg)
     gene            complement(20006..20079)
                     /locus_tag="IWO50_001958"
     tRNA            complement(20006..20079)
                     /locus_tag="IWO50_001958"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20045..20047),aa:Trp,seq:cca)
     gene            complement(20086..20166)
                     /locus_tag="IWO50_001959"
     tRNA            complement(20086..20166)
                     /locus_tag="IWO50_001959"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20130..20132),aa:Tyr,seq:gta)
     gene            complement(20168..20243)
                     /locus_tag="IWO50_001960"
     tRNA            complement(20168..20243)
                     /locus_tag="IWO50_001960"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20208..20210),aa:Phe,seq:gaa)
     gene            complement(20279..20351)
                     /locus_tag="IWO50_001961"
     tRNA            complement(20279..20351)
                     /locus_tag="IWO50_001961"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20316..20318),aa:Asp,seq:gtc)
     gene            complement(20361..20433)
                     /locus_tag="IWO50_001962"
     tRNA            complement(20361..20433)
                     /locus_tag="IWO50_001962"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20398..20400),aa:Val,seq:tac)
     gene            complement(20449..20520)
                     /locus_tag="IWO50_001963"
     tRNA            complement(20449..20520)
                     /locus_tag="IWO50_001963"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20485..20487),aa:Glu,seq:ttc)
     gene            complement(20535..20624)
                     /locus_tag="IWO50_001964"
     tRNA            complement(20535..20624)
                     /locus_tag="IWO50_001964"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20588..20590),aa:Ser,seq:gga)
     gene            complement(20664..20737)
                     /locus_tag="IWO50_001965"
     tRNA            complement(20664..20737)
                     /locus_tag="IWO50_001965"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20701..20703),aa:Asn,seq:gtt)
     gene            complement(20747..20862)
                     /gene="rrf"
                     /locus_tag="IWO50_001966"
     rRNA            complement(20747..20862)
                     /gene="rrf"
                     /locus_tag="IWO50_001966"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6600 a         4390 c         3332 g         6617 t
ORIGIN      
        1 gaacaatagc cttttattat atatattatt caaattagtt atatcgagtg aaaaaaactt
       61 tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt tcacttacat
      121 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
      181 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      241 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      301 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      361 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      421 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      481 atgattattt caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat
      541 caaatatact tgtcattcat catgaaatat agagacttat aaaattgttt atttttataa
      601 tgtatcagta tagaaaggaa ataaaacatg gattatactg ttgaatttga cagtgaacta
      661 cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc ccacaaagtg
      721 acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa
      781 ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc atttgcttta
      841 aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat tgtatttgat
      901 aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga gtgatactaa
      961 gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt acgttattat
     1021 atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga cataagaaat
     1081 accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt ttgttttctc
     1141 agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct tctccaaagc
     1201 ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa tgaggatatc
     1261 ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga taatcttccc
     1321 ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta accttgcagt
     1381 gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct catcgatcat
     1441 aagggaatca atattcattt ttttcttcct ttcttataca attctccctc taataataca
     1501 gttctttgac tatttaatct atcacatata actctcctgt aaagattctc tcgattcctt
     1561 aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt tgtgataaat
     1621 tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc tacccttacc
     1681 ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat aatgtttatc
     1741 tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt ctttggccat
     1801 cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg aatcccccca
     1861 tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat ctaattttct
     1921 ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt ctcttacttc
     1981 ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag catgagtcgt
     2041 gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg tggaatgagt
     2101 atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg tggggagatc
     2161 tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg ttaccaccat
     2221 ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga tgactcctag
     2281 ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt atcgcggcaa
     2341 tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt cacaagacat
     2401 catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg atttctcttc
     2461 gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca taatctagac
     2521 gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa ggaacatcac
     2581 gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg atagcagtga
     2641 tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat gcggacaaat
     2701 tgcctaggtc tggatatact tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa
     2761 attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga atgatgccat
     2821 atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct ctggaattca
     2881 ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg acgcttagat
     2941 tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc ttttctggat
     3001 cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg atacgtacac
     3061 cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg
     3121 ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc atttctttta
     3181 caagggaaaa acgttctttc catgaaatat ctttgggtaa cgctttttca taaatcccaa
     3241 tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt ttgtgcggcc
     3301 ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata cacatcgatc
     3361 ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac attaaagcct
     3421 agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc accagataac
     3481 agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg ccaatccatg
     3541 gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat ttctttttgt
     3601 gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc tgctcctgct
     3661 tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc agcgactagt
     3721 tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa
     3781 agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac atctcctaat
     3841 gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct catcttagtt
     3901 ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca ctgatttctt
     3961 tgactgacat cacatttttg ataccgataa ctgtaactcc ttttttctct gcctgagtaa
     4021 acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct gaactttttc
     4081 cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct ttcattgctt
     4141 tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac gatactaaaa
     4201 ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt tatactctct
     4261 gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccatca ggatttttcc
     4321 gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt ccagcataac
     4381 ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg aacatcccga
     4441 tataaccacc gtatgttgac atcccaatcc atgtagcgaa taatgctgaa ccgcctactt
     4501 gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct cggttattgg
     4561 cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct atgacgatcg
     4621 ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca
     4681 agaacccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct acgtcaattc
     4741 ctggaacagc tcctggtaaa atcgtgtttg agattccttg gaaagattga gttagttcat
     4801 cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa tttaaagaag
     4861 tcgtcataat gtagaataag aaactctgcc cttcttgcat gaattgcgct tgaatcaaat
     4921 aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt aacgaagtag
     4981 caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct tcaatttttt
     5041 tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac tcagctaatt
     5101 ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg gcaccttctg
     5161 tcaattcctg cgtgattcct actgttaagt tagaagctac tgtccaataa caacctaaaa
     5221 tcagtcccat aaaaatcaat gcttgtatcc tacctagatc aggaaagcaa aacagtaaaa
     5281 tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca
     5341 gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca atcagtaata
     5401 aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca tccacagcat
     5461 tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc tttagtccaa
     5521 ccaagattgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa ataaagtaac
     5581 cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc agcaaaatat
     5641 agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata tttgttgcaa
     5701 aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat caattaatta
     5761 tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag acgctcaacc
     5821 aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg ttcttctgga
     5881 tcttttgctg cgagtgtaaa aaacaaacta gccgttttct cctcattctc cccttcactt
     5941 tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa aacaccatca
     6001 cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata aggaccgtat
     6061 ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc cttatcgatc
     6121 aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt atctgggtaa
     6181 tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt tcttataaaa
     6241 acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct cgataaaaat
     6301 taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact tgccagatat
     6361 aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca gtgatctctt
     6421 ttggatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact acttttgcat
     6481 tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt atccctcgtg
     6541 ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca tgtttggcaa
     6601 ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat gtttcgaata
     6661 aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat acctcttctg
     6721 gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact acattgatgt
     6781 ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca ggaattcccc
     6841 atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca ggacaatttt
     6901 gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt gtaacgatta
     6961 aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta cgtaaattag
     7021 gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc atcatgccat
     7081 tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc gttccttcat
     7141 gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt ccaggatcca
     7201 ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat gtttctacta
     7261 accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc cccccatatc
     7321 gttgttccaa cttacaagta aatattaatg tgtatcagca tccatgaaaa cccttttata
     7381 tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc agtataataa
     7441 agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat caagatgtcc
     7501 ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa
     7561 ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct gaatcccttg
     7621 atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa aacgagatca
     7681 tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt gatcaatctg
     7741 gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt aacgattatg
     7801 atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt cttcaatcta
     7861 accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac aaagataatt
     7921 gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta gtgggcgaca
     7981 tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc tgtagcggag
     8041 tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc aacgaaatga
     8101 tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa atcggactga
     8161 catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact gataaatttt
     8221 gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt caagtagatt
     8281 cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg aataacaaat
     8341 ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca agatgtatcc
     8401 atccaatcga taccatcata atctattgct tcttttataa ccgttatatg ttgcctaaat
     8461 aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa
     8521 taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg tcgttttcaa
     8581 tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg tccatatatc
     8641 tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca taaagcaagt
     8701 attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc tcacataatt
     8761 ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca taatatacaa
     8821 tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta cgctccacat
     8881 aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg tttgcagctg
     8941 cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta aaataaaata
     9001 atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa aataaaattt
     9061 gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa attttataag
     9121 attcttggta ttttgataca gaattcaatg attgaatctc atttattttt ttcctgattt
     9181 tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt ttgcaaagcg
     9241 cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg acagttgctt
     9301 gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt cggtattctt
     9361 ttagtaatgc tccatctact ttaatcaaaa caatttcctt ccctttaaaa aattatttct
     9421 cctttatatc atacacaaaa aaaagttcta aacaaccaaa aatgtttaga acagaataaa
     9481 aattttaatt ttaaaataat attcaaatta aacaactggc gtatctgctc cttgatttct
     9541 ttttgtgtta tctgaataat tatcgtttac agttataaca catagtgaaa attgtcatgg
     9601 cgagaaagtc taagaatatt tttaaacata actatttctt tcttacgtgt gtataatgac
     9661 gacagcacac ctatcaatta atataattat tttatttttt tatgttctca tgtttatttt
     9721 taatgacaca acttctcgta tttatctggc acaacatata aatcgatcaa aaaattgttg
     9781 cgtgtttttt tgaaaattgg agtttcaact tgattacaaa atttaaattt agaagttgtg
     9841 aaaacagcga gagacagttt aatattcaaa atatacatag gctactcgaa cgctgtgttt
     9901 tttagtatac tagtaagaaa cgaagggata gactcattcc aataaggagg atttcatgaa
     9961 gacaattctt tttgctcctg cagtattcaa tttagctgag acaacacgaa tgattgaggt
    10021 tgccaaagct atgaaagacc gttattactg tgtattcttt ggcttttcta aaacttttgc
    10081 tcctttgatc acagaaagtg gctttgagta tcatttacta gcgccgatcc ttacgaaaaa
    10141 acaagaagaa caaatcataa aatttgacca gttgcgtaca cttaaaaacc cttttaccaa
    10201 agaaatggtt gcctctcgta tcactagtga acagagtctg attgctaagt ttgagcctcg
    10261 actgattgtg acaggttcga atgtgacaat ttttttatcg gcccgcaata aaaagattcc
    10321 tttagtatat gttaaaccct atgctttgag tgaacctttt tttcaatcag agcatgcgct
    10381 gatgccccac gagttacgtt tcttcggccc tttaagcaat cccctttggc actggacaaa
    10441 aaagcgtata gtaaataccc gatggatccc caatgccttc aaacacgctg cacaaaaaga
    10501 aatgcttcct ttaccaaaaa caagtttaca aatgatggat gctgatctaa atctcatcac
    10561 aacgcctaaa ctatttttgg aagaagaaca gctgccggaa aattatcgaa tcgttggtcc
    10621 aatttttgct aagctcagta cttccttgcc tgaagaagtg ctaactttta tacagcgaaa
    10681 gcaacaagaa agaaaacgta ttatctattt cgcaatgggt agttcaggca atccgcgtat
    10741 gttacgcaaa attttgttct tcttacaaaa acaaccggat ctagcggtta ttgcacctat
    10801 tgactatctg cttgatgaca ttcaatctga ctcagaagat cttttaataa cagaatatct
    10861 gtctagtcat cttttgcatg aattcattga tttttctttt atccacggcg gggaagggac
    10921 cgtccaaact gcctgtgaat ccggcaaacc gtttatcggt gtggggatgc aatttgagca
    10981 aagctgtaat attgactatt gtgtaaaata cggaaacgca atcgccctca caaaaccagt
    11041 gactgaaaga aaactgcaga ttgctctgcg cgaagtttct tcacccaaga ttcgtcagca
    11101 ggcacgcacg ctgcaagccg tttttccaat aaatggtcca caacaagcaa ttcaagaaat
    11161 cgaaaccttc ctgagcaacc acccaatata gtttcaagta gaaaaagctt ttttaataga
    11221 gactcatcag taatttcgct attcgagacc cagacagtga aaatacaaaa aaagtaaata
    11281 gtggattgaa aagttagagt tacttaaatt acggtgtcta aaaaacacaa aatcatcctt
    11341 aaaataaaag atctgacgct atccaaatga gcataggtag cgtctgccat ttattcttag
    11401 tcaaattctt tgacaccttt ctctggttgg atcatctcaa cgtttcttca aaagaaagac
    11461 cataataatt aggcaaatac attttcttaa aatgtgtcat atagatctag aagcatcgct
    11521 ttgtagagat ttgtgccgct agtcccctca acaattacta aatcaaatgt aggatttacc
    11581 actttatctt tgataatcaa cctccctctt ctgttgagac aatgatctcg caatccgatg
    11641 catactccta tataaactgc cagcaatccc cacatattga gtagatcatg tcagaatgtt
    11701 ctatgtcgtc agcaacgata cactaaaagt cacgttcacc atgactgatt gctattccca
    11761 gcacaattat ttctatacac gtttcatgat ttgaataaac actcacatca tacatattac
    11821 cattcataca ccatactgca gcccctacta tatggttgta agctcccaaa tctatatttg
    11881 acttgatcac tttggtagta gatctactaa ttctaaatcc attgctgata aaatttccat
    11941 tttttcctcc ttctttctgc caaaacagtt aaaacaataa taatctttat gtaaaaaagg
    12001 gctgtgacaa aagccttgtc acagtcccat attccgaata aacggtggtc aaaagcttgc
    12061 atctgcggta ataagttcaa ccaaacgaaa aataagccaa catttcacaa aaatttaaga
    12121 agcaattttc gtaaatttct tcttatttct tgaagctaac cacttctgtt acaaccactt
    12181 ttagaacatt taaagcttcg atgtgacaaa ccatctatgc tgctcgatca taaaattgat
    12241 agcaaaaatt ccacaaagta catatgtccc ccatgaaaac aaaacattca tatgcggtac
    12301 aatgatcttt ggaagaatca cgatcaaaaa tgatgccagt gcccaaatcc atcgattgat
    12361 ttgaatatga tatgtagcta gcatattcaa tataaggtga ctagccgtta ataaaaagat
    12421 aaaaacaaaa aataaaatca gccaatacat tgtttcccac cttctaaaaa ccaaacttag
    12481 acagtctgga cttcgttttc tccacgactt tgttcatctg acagcatctt tttatcataa
    12541 gcttttacaa atggataata gataataaat gctatcaatg tgttgatcat gatcaagata
    12601 acaacacgcc agtcatttcc tgctgaaaga taaccgccga ttggagctgg taatgtccat
    12661 gttggcaaaa cagtaaatct attgaccatt tctaatttga ctgcaaaata actgataata
    12721 gtcaaaacta aaggaactaa gttgaacggg atgaacatgt aaggattcaa catcatagga
    12781 gctccaaaaa ttatgggctc attgatattg aagataccag gaataataga aaaacgacca
    12841 acctctttca agaattttga tttacagaag aatataaaca aaacagatac cacgatcgtt
    12901 gctcctgcac caccaattgt taccgtccat tgataaaact gttcaggagc gatatagggt
    12961 aattgggtgg cagctgtacc atctgccaat gcctttgcat tctcatccaa catgatcagc
    13021 cacattggac gaacaacagc ccctacaata ctcatgccat ggataccaaa tgaccacaac
    13081 aaatggatca aaaacattgg tagtaaaaca ccaagcaaat tattccctaa aatattggtt
    13141 aaaggctcga ataacgataa taaggcagcg tttacatcaa agcccgcgac gcttcgaatc
    13201 agccaagtaa ttgttaaaat gactgcacct ggaatcaatg cctcaaaact acgggcaaca
    13261 gcaggtggca cttgttctgg cattttgata gtaatatttc gatttttaaa gaaacgatat
    13321 acttccactg caaaaatcat agataagatc cccgcaaaca tcccagatgc tcccaaagaa
    13381 ctcataggta agacccagcc aagaggtgcc tcttcaggag ttaaaacgtt tacaggaact
    13441 tgtaacataa agaacgttcc taaagataat ataccacctg tcacactatc taatccatat
    13501 gatttagcta gatccgaacc cataacaaaa gttgcataaa tagccatcaa tcccatcgtc
    13561 atcctaaagg gtaaaacaat cgttgccgaa taaggggcaa taaattcatt ccatccagga
    13621 aacggtaaat tgataaagac cataaaaaat gtcccaatca aaatcaatga caatgttgaa
    13681 acaaccccat tacgaattgc taataaatga cgctgtgtac caatttttgc cattggtggg
    13741 acaagtttct cttcgaccca tttcatcagc ttttccacta aaaccactcc ttttcaataa
    13801 aatatgaacc attcattgca tgacaacgca aagaatgttc ataactaatt atataattaa
    13861 gaaattttat ttcaatatat tctcttaaat aatcaaattt atttttaaga atcctgaaaa
    13921 taagttttct aaatcatagg agcaacaaaa aaattcgata attaacaaaa atcaaaatga
    13981 ttaaatctag tgattgaaac atatcatttc aaaaaaattt tgtaaattca cccataaata
    14041 tcaccctatt taactttttt catcgtacac taaaattcaa aacaactatc tttaatcttt
    14101 acttcttggt tttgttttaa taaatttcct tcaaaaatgc ctggagaaac tacaatgatc
    14161 accgtatctt tttgtgcttg ctccaacttc ttcaaatcta taatagctaa cagtgtccct
    14221 tcttttacct cctggttact ttcaacccgt atctgaaacg gacttccttc gagttcaact
    14281 gtatctaaac ccatatggat cagtattttt ctgccagatt cactttcgat cgttatggca
    14341 tgtcgggtag gaaatatatt atcgattatt cctttaactg gcgcatggat atatccatca
    14401 tgattcgtta tagcgtatcc aactcctatc atttttgaag aaaagacctc gtcttttact
    14461 gctgttaatg gaataatttg accactagct acagctttta gaatgtttct tgttttaaag
    14521 aaatgcactt tttatcacct ctactttaat gctggccaat attttttgtt tactttgatc
    14581 atttcatcta gtattttttt tgcgactctg gcatttggta cggtttgatt caatgtaaat
    14641 gcttgtaaag ctttttgata agaaccttca aagtaagcat ctactagtaa cttctctact
    14701 gcaacttgtg cctccatcaa tcctttatga aaatcactga ttggttttct tagtgtaatt
    14761 ggctccacac cagttgcacc aacatatgcc ggtatttcaa caactgcatc tgaccgtaga
    14821 ttcggaatcg ccccttgatt aggaacgatt agcataaagc gacgacgatc atcattcaac
    14881 aaggaaatag ctatatcgac gatatattgt ccatgctccc caaaattaaa gttcaatact
    14941 tcttccacaa taccgcggcg tattttatca gccatttctt tggtattttt ctctcgtcca
    15001 tccatgacca tattagcacg tgtatactct ttgtcagtat gctcaacgac catatcagga
    15061 tataagtaat actctaagta attattcggg atattacttg gaaaattttt cgtgatgact
    15121 gacatcattt gaaaggtttt ttgccaagtt ttatctccta tattaaaatc ggcgacttgc
    15181 atttcttttg ttattagttt ttcaataatc tctggcataa tttctcgttg caattcttta
    15241 tcataaatcg atgtatacca gccaaagtgg tttaaaccat aatacgtaac gatccaattc
    15301 ttacgatcat aaccgtaatt cactgcaatc gtttcttcaa ttgaaatagt catatcacag
    15361 gcattaatca tttttacatt cggatattta cgacgaacag cttcagaaac gatcgactct
    15421 ggatttgtat aattcaaaat ccatgctttg ggggcatagt cttgtatatg atcgacaatt
    15481 tccaataaac ctttgattga gcgcatccca taagcaaatc ctccgagtcc acacgtttct
    15541 tgtcctacta atccatgttt aagtggaatc ttttcgtcct tttctcgcat ctccaatcct
    15601 ccaacacgaa tttgtgagaa aacaaaatcg catcctgtaa aagcaagctt tggatcatcc
    15661 gttgcacgaa tgcgaatatt tgaaaaacct tttcttttca gcataaattt aacaatcaag
    15721 tacatatcca tatttctttc ttcatcaata tcatataaac gtatttcaga taatggcaac
    15781 cgtttttcat tgttcaaaat ggcttgaacg attcctggtg tatacgtact cccgcctcca
    15841 gctatagtga taatctgttt tttcatcatc atcttttcct ttctattctt ctaataattt
    15901 attgaattct gccataacat aagtcacttc taagccgatg ataatttgat aatttttttc
    15961 agttggttta atgatcccgc ttatccctaa ttgcttgatt cgctgttcct cgaccatggt
    16021 gggatccact acctctaccc gtaggcgtgt attacaacta tagacatcca ggatattggc
    16081 ttttcctcca aacgcttgta gcatttttgt tgccatatat tcataatttt tacttgctaa
    16141 ccctaactct gtttcttcta aggttacttc attgccaaac tctgccttag cttcacgtcc
    16201 taatgtctga atgtttctct tattgatcaa ataataaaaa gtaacgtaat acaaggcaaa
    16261 aaagaaaatt ccgattggaa gaatcaaaat agcattggta gcgattcgaa aattgatgag
    16321 ataatcaaca atacatgctc cgaatgtaaa accaattcgt atattgaata agtaacagac
    16381 aatccctgct aaaccggcaa aaagtgcatg gatcacgtac aacataggag aaagaaacat
    16441 gaaactaaat tcaacaggct cagtgatatt tgcaataaat gaagttcctg caccgcttcc
    16501 catgattcct ttgaccatct ctttattttc tttataagct gctttataga tagctagaca
    16561 tatagctggt ataccaaaca tcattactac aaaaaaaact gataaaaaca gtccagcctg
    16621 aggatctccg ttgataaagc gtgtcatctc tcctctgaaa acttccttgc taggagatgt
    16681 atagcttcct aaatcgtaat aaatatacgt attcaacaca tggtgcagtc caaaggggat
    16741 cagcaaacga ttcaaaaaca taaatatccc cacacctaat gctcccatat caacgagcag
    16801 ttttgcaaaa ttatcaatcc caatttggat gagcggccag ataaagccca aaaaaacagc
    16861 tgcgattgtt tgtagaatca tcaccatagt caatgggaat ttttcgcccg caaaataaga
    16921 aaaaactact ggtaattttt gttctttaaa gcgattatac gtccatgccg ctactaaacc
    16981 ggaaataatt ccaccaaaaa tccccatatt cacttctggg tccataattt ctaagccttt
    17041 tttcaaggta gcaatcgcta aaaaaccagt aagtgcaggt attcctttgt ccttattttt
    17101 gacaaaaccg attgcaactc ctatagcaaa taagatatcc aagtttccaa aaacggcatt
    17161 tccagctgca ccaattactg ggatgttcag catatcgtca gctgataaac gcatgatcaa
    17221 tcccgctaat ggcatcgctg caactggaag tagcatcgct cttccaagct tctgcacatt
    17281 tgatttaaca ttcattttta ttcctcctct attaaatatc ctcatatgtt actaactcaa
    17341 tcaaaggatc atttttcact ttttgagcaa atgctgaatg aattaaaaat tcgacttggt
    17401 gggtacgagg atacacaaaa cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt
    17461 gacacatgac ttcgatcgtt ttatcttctc tcaatatcat gagtaaactt gccaaatatt
    17521 cttccacact cgtattgatt tgtcgttttt tttcatcagt ttctcctacg acatcgaaac
    17581 aaggctcaaa ataatcgaca tgacgaactt catcttttcg ttcaaagttg ctccgaaccg
    17641 gcaaatcata tttttttgct aatgtaacaa ctacctcctg attttcatcc aaagtatgag
    17701 tatgatgatg actgtctaaa tgagttggta tgatccccgc atgataaatt ttttgaatct
    17761 gagcattcca ttcctgatac agttgatctt tgtcaatgtt aaatggatgc tgataataag
    17821 ataaggatct gaactctccg ctttcgataa gagtatcgac agttttgagg agtggtttgc
    17881 cacacgtcaa tgttaaatgg actcctacac ctagcctagg catttccttt cttagtttca
    17941 cagcatgctc gaaacccggc atatttgcca tcaatgtagt tgatgtcaaa attcctcgct
    18001 gataagcatc cataatccca taattcacac catggctata gccaaaatca tccgaattaa
    18061 aaatcacttt tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata
    18121 atcatagtcc atttactatg tgtcatttgg ttgcgcctat agatagttac gcccttattt
    18181 taatagattc ttgctagatg aattgccaaa tagatgattt cattttcttg aatggcaagt
    18241 tgaaattgct gttcgatgat atctttaatc tttagtgctg tacaatacgc tttgctatgt
    18301 ttaaatgttt cagtaaaaaa accaatattt tctactggcg tttcttcctg ataaatcaag
    18361 cgtttaacca ggaatttgat atgaaccatt aagcgatgat aagtcagtga ttctttatca
    18421 taaacgtttg aataggattt ttctagatgg gacaaaatcg tggtcgtcat ttctactatt
    18481 tttaagctct ttaaactatt aggctctcct agctcagcat taatgatatg catagtgatg
    18541 aatcctactt catttgaccc taactcggtt tgatatcgat gattgattcg ctcgaccact
    18601 tctactgcta tatcatattc atttggataa aggaatctca tttctggcaa cataattgct
    18661 gtgacatctc tatcttctct ttttcgttga atagcaaaat aaatatgatc tgtcaatgta
    18721 atatagatat ttgaatctaa tggagaggag atcctagctg ttatcagttc aatcacttct
    18781 tccgcaaaat ccataaattc gattggaatc tgcgagacta tctcactgaa tcgtttgatc
    18841 ttttctttag aatctaatac aaataatttg tcagcatcct caacatcaaa tggcttactc
    18901 actttagcag aaaagccaat ccctttcccc atcgcaattt tttctattcc atcgtcatcg
    18961 atcaatacag cattatgatt cagctttttc atcacataca ccgttaattc acctcctcta
    19021 tatgataaaa caaaacaact tgcagactcc ttcatacata tttttgtctc caaacaattt
    19081 ttaaagacaa aaaacgacct aagaaagaac cctttcttag gtcgtgccta attgaatagt
    19141 agcaccctac ataatttaca aaaatagttt aacacataat aaaagcgtta tcaatatctt
    19201 ttttactcgt tttatgataa tacttgtatc gaattgattt ggaggtaatt acataaaaac
    19261 aaaaaaaata tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc
    19321 ggggtcgaac cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat
    19381 tccgccacgc cggcaaattt aaaaataata actggggtag ctggattcga accaacgcat
    19441 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa agggcgaatg
    19501 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
    19561 ttcgccatca taaaagatgt tgtttaattt ctcaataaaa aactaagatg aagacatctt
    19621 agtcaatacc ggcggccggg gtcgaaccgg cacgccctca acgggcactg gattttgagt
    19681 ccagcgcgtc tgccaattcc gccacgccgg caagttgatt tattagtact aaggcggtaa
    19741 ccggatttga accggtgatg aaggttttgc agacctctgc cttaccactt ggctataccg
    19801 ccattatact attatcaatc tataaaataa agaataaatt atagaaactg gggtagctgg
    19861 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
    19921 gaataaaaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc
    19981 gttaaccact tcgccacatt cgccatggca ggggcagcag gaattgaacc cacactaacg
    20041 gttttggaga ccgctgttct acctttaaac tatgccccta taaaatggag gagagtggat
    20101 tcgaaccact gaaccctaag gaacggattt acagtccgtc gcgtttagcc acttcgctac
    20161 tcctccatgg tggcgcggga cagaatcgaa ctgccgacac acggagcttc aatccgttgc
    20221 tctaccaact gagctaccgc gccatatttt aaaattatta attgaataac aagaacgacg
    20281 gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac
    20341 accacggaac cagttcatta tggaggttaa cgggatcgaa ccgctgaccc tctgcttgta
    20401 aggcagatgc tctcccagct gagctaaacc tccaacattc aattaaaatg acccgtacgg
    20461 gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc
    20521 aggtaaattt gctacggaga gtaagggatt cgaacccttg agacagtttg gcaccatcta
    20581 catgatttcc aatcatgctc cttcggcctc tcggacaact ctccagagtg aaagaagctc
    20641 aatactcttt ctcaaaatga gaactccggc agtaggactc gaacctacga catcatgatt
    20701 aacagtcatg cgctactacc aactgagcta tgccggaata acatcagcgt ggcgacgtcc
    20761 tactctcaca aggggcaacc cctcactaca atcggcgcta agaagcttaa cttctgtgtt
    20821 cggcatggtt acaggtgtat ccttctcgct atcgccacca cactgtggtg ttatctttta
    20881 ttgagtaaat tttgttcact caaaactgga tttgaagtca cataagtttt tttccgagt
//
LOCUS       DACEDM010000057        20799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEDM010000057 DACEDM010000000
VERSION     DACEDM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.057"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWO50_001967"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWO50_001967"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0359074.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IWO50_001968"
     CDS             complement(2261..2512)
                     /locus_tag="IWO50_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0359075.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IWO50_001969"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IWO50_001969"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0359076.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IWO50_001970"
     CDS             3461..3679
                     /locus_tag="IWO50_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359077.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IWO50_001971"
     CDS             complement(3968..4480)
                     /locus_tag="IWO50_001971"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0359078.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IWO50_001972"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IWO50_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0359079.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IWO50_001973"
     CDS             complement(6933..7367)
                     /locus_tag="IWO50_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0359080.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IWO50_001974"
     CDS             complement(7377..8165)
                     /locus_tag="IWO50_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0359081.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IWO50_001975"
     CDS             complement(8162..9103)
                     /locus_tag="IWO50_001975"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0359082.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IWO50_001976"
     CDS             complement(9280..9867)
                     /locus_tag="IWO50_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0359083.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IWO50_001977"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IWO50_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0359084.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IWO50_001978"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IWO50_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0359085.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IWO50_001979"
     CDS             complement(12784..13719)
                     /locus_tag="IWO50_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0359086.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..14864
                     /locus_tag="IWO50_001980"
     CDS             13905..14864
                     /locus_tag="IWO50_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0359087.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14950..16044)
                     /locus_tag="IWO50_001981"
     CDS             complement(14950..16044)
                     /locus_tag="IWO50_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0359088.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16233..16994
                     /locus_tag="IWO50_001982"
     CDS             16233..16994
                     /locus_tag="IWO50_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0359089.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="IWO50_001983"
     CDS             complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="IWO50_001983"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0359090.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17769..18524
                     /gene="fabI"
                     /locus_tag="IWO50_001984"
     CDS             17769..18524
                     /gene="fabI"
                     /locus_tag="IWO50_001984"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0359091.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18634..19647)
                     /locus_tag="IWO50_001985"
     CDS             complement(18634..19647)
                     /locus_tag="IWO50_001985"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0359092.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19812..20732
                     /locus_tag="IWO50_001986"
     CDS             19812..20732
                     /locus_tag="IWO50_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0359093.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         6000 a         4468 c         3685 g         6646 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg
    14401 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
    14461 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
    14521 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
    14581 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
    14641 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
    14701 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
    14761 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
    14821 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
    14881 aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata
    14941 actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc
    15001 aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt
    15061 catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat
    15121 cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta
    15181 gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg
    15241 aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt
    15301 caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc
    15361 gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg
    15421 tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca
    15481 agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct
    15541 gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata
    15601 aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac
    15661 gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat
    15721 ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt
    15781 atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat
    15841 atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga
    15901 aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc
    15961 atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata
    16021 aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat
    16081 ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa
    16141 taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag
    16201 ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaagta
    16261 ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac acgacttctt
    16321 ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg actgccagta
    16381 gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc gaaaatgaag
    16441 atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt cccgaacacg
    16501 caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac aacggactga
    16561 ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa ggcgatggca
    16621 ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac cacctttata
    16681 atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac ggcagccagt
    16741 tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac gactatcctc
    16801 aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc cattatactg
    16861 tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat atcgaaacag
    16921 atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata ctgcaagaag
    16981 caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt atgctaggat
    17041 tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg ctatatcagg
    17101 ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca gactttttta
    17161 tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt ggtgatttca
    17221 aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc cttattgatc
    17281 ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag tatcgaacct
    17341 gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc aggaaagtga
    17401 ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt ttttcctggt
    17461 tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat caattccatt
    17521 acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg caattgccat
    17581 tttcattact ttgactatca aagtatcata ttcctaaata tttagcaatc tttttttaat
    17641 ttaagaaaaa atagtttgac aatcaaaaca ttttgactaa taattaagaa atcaagaagt
    17701 tattttagct attattctct atttttttac gactatcaaa tttttatcag aaacaaagga
    17761 gagtcattat gtcatttcta gaaggaaaaa aaatcgtcat tatgggcgtc gctaacaaaa
    17821 aaagcattgc ttggggttgt gctgaagcaa tgttgaaaca aggggcagaa attatctata
    17881 cttaccaaaa tgaacgaatg aaaaaatcat tagtcaaatt ggtcggcgaa gaagcattca
    17941 ctgtagaatg tgatgtttct agcgacgaaa gcatcgaaaa agcaatgaat gccatcggtg
    18001 aatatgctgg ggagatccac ggccttgtcc atgcagttgc ttacgccaac aaagaagaat
    18061 tgtccggaaa tgtgtctgat atttccagag acggctatca attagctcaa gatatcagca
    18121 gctactcttt aattgctgta tcaaaatacg cacaaccgtt tcttgcacca aacagcggga
    18181 tcgttagcat gacttatcta ggttcagaac gcgcaatccc gaattataac atgatgggta
    18241 ttgcgaaagc ttctcttgaa gctgctatcc gttatttggc agctgagttc tcgcctaaag
    18301 gaattcgggt caatggaatc tcagctgggg caatcaaaac attagctgtc acaggtgtta
    18361 aagactatca aaaactgatc cagttatctg aatctcgtac gcctgatcag caaggtgtga
    18421 cgatcgaaga agtcggtaat gcttgtgctt tcttaatcag cccactttcc agcggaatca
    18481 tcggtgatat tatctacgtt gacaaaggtg tccatctttc ataaactttt gttgcattct
    18541 ttaaagtaaa acatataaaa atcaggaaaa aactttttaa agtctcttcc tgattttttc
    18601 tttttacgtt tttttcaact cattactttg aaattattcc acgacttcaa tccagccttc
    18661 tggtgcttct atgtctccaa attgaatacc tgtcaattca tcatacaacc gttttgttac
    18721 tggtcctact tccgtttcac tataaaatac atgaagattg ttattgtgat aaatcccacc
    18781 gattggagaa ataacagccg ctgtaccaca tgcaccagct tctgcaaatt gatctagatc
    18841 atcaatgaaa atatctcctt caattgcttg cagtcctagt cgttcttcag ctaactgcaa
    18901 caaggaatat ttcgtgatac ttggtaagat agagggcgat tgcggagtga tgaattttcc
    18961 gtcttttgta attccaaaga aatttgctgc acctacttct tcgattttcg tatgtgtggc
    19021 tggatcaaga taaacacaat ctgagtaatc cagttctttt gcttctgttc ctggaagtaa
    19081 actcgctgcg taatttccgc cgacttttgc tgcaccagtc cccttaggag cagcacgatc
    19141 gtaatcagat acaataaaat tcgttggttt tagtccacct ttgaaatacg ctcctactgg
    19201 cgtacagaag acgacaaata aatattctgg agctggatgg acaccaatgt tttcaccgac
    19261 cccaatcagt aaagggcgca agtataatgt agcgcctgtt ccatagggcg gcacaaaatc
    19321 ttcatttgct tttaccactt gcttgacagc atcaacgaac atcttttcag ggaccattgg
    19381 catcagcaat cgtctagcac tttgattcat ccgtcgacta ttttgttcag gtcgaaagag
    19441 attgaccttg ccgtcttttc gacgataagc ttttagacct tcaaaacatt gctgaccata
    19501 atgaagcgct ggagagcctt catgaatcgt caggtaatta tcttctgtca attctccttc
    19561 ttcccattgt ccgtctttcc atttggcaat aaaacgccaa ggagttttga tataagaaaa
    19621 acctagattg ttccaatcga tttgcatata cttaccacct tcgttatttt ctccacattt
    19681 tatcattatc catattattt gtcattttat tttcagcttt tttccttcat tatagttatt
    19741 tttgctattt ccttttttga aaacttcttg gtacaataca gaaagaatca aattttagaa
    19801 aagaggattt tatgttttca ttattagcaa ctgccggtac gacagactct tctggcgaga
    19861 cattgacaga tcaagctgtc agcaaagtaa atgcgtttca gcggttttgg gacgggattg
    19921 attgggacgc cattttggct gcctttatcc aaaaaagttt atatctgatt tttttgatcg
    19981 tccttttttg gattcttcac cgtgtgggaa aatatctgat tgataagtct tttaatcagt
    20041 attctaaaaa agccattcta aacgagagcc gcttaaagac tttacatagt cttttaaata
    20101 ctatttttca ttataccctt ggtttctttt ttatctatgc aattttatct gctatcggtg
    20161 tgccgatcgg atcacttttg gctggtgccg gaattgcagg tgtggcaatc ggtcttggtg
    20221 cccaagggtt catgagtgat gttattaccg gattttttat tatcatggaa caacagatgg
    20281 atgttgggga ctacatcaag ctagcaaact taacgattga aggaaccgtc gtatctgttg
    20341 gtatccgtac ccttcagctg aaagcaacgg atggtactgt ccatttcatc ccgaatcgaa
    20401 acatcaccac gatcagcaat ctctctcgtg caaatatgca agtactttta gatattcgga
    20461 ttgttcctga agaaggatat gacaaaatct atgagatcat tgatcgagta aatcgtacat
    20521 tagctgaaaa ataccaagaa gagctccaaa ctgaaccttc tatctttgga ttagtggatc
    20581 ttggaaacag caattttgcg attcgtacgg tctgctacgc attgaacgga aaacaatatg
    20641 ccttaaaaga agagttcttg agctcctatg tcaaagaatt gacagcttct ggtttcacga
    20701 ttccgaacag tccaattgct gtcggaaaat aatttggaca aacgatgcaa cgaatctcac
    20761 taaaaagagc gtgggacaaa agtaaagaac acttttgtc
//
LOCUS       DACEDM010000058        20756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEDM010000058 DACEDM010000000
VERSION     DACEDM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.058"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..546)
                     /locus_tag="IWO50_001987"
     CDS             complement(<1..546)
                     /locus_tag="IWO50_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0359094.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEE"
     gene            complement(660..1991)
                     /locus_tag="IWO50_001988"
     CDS             complement(660..1991)
                     /locus_tag="IWO50_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0359095.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(1957..3435)
                     /locus_tag="IWO50_001989"
     CDS             complement(1957..3435)
                     /locus_tag="IWO50_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0359096.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(3740..5575)
                     /gene="tdc"
                     /locus_tag="IWO50_001990"
     CDS             complement(3740..5575)
                     /gene="tdc"
                     /locus_tag="IWO50_001990"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0359097.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            6045..7472
                     /locus_tag="IWO50_001991"
     CDS             6045..7472
                     /locus_tag="IWO50_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0359098.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(7564..7929)
                     /locus_tag="IWO50_001992"
     CDS             complement(7564..7929)
                     /locus_tag="IWO50_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0359099.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(7926..8291)
                     /locus_tag="IWO50_001993"
     CDS             complement(7926..8291)
                     /locus_tag="IWO50_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0359100.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(8483..8731)
                     /locus_tag="IWO50_001994"
     CDS             complement(8483..8731)
                     /locus_tag="IWO50_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359101.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            8730..8858
                     /locus_tag="IWO50_001995"
     CDS             8730..8858
                     /locus_tag="IWO50_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0359102.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            8915..10609
                     /locus_tag="IWO50_001996"
     CDS             8915..10609
                     /locus_tag="IWO50_001996"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0359103.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            10709..10882
                     /locus_tag="IWO50_001997"
     CDS             10709..10882
                     /locus_tag="IWO50_001997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359104.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            11260..12393
                     /locus_tag="IWO50_001998"
     CDS             11260..12393
                     /locus_tag="IWO50_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0359105.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            12424..12744
                     /locus_tag="IWO50_001999"
     CDS             12424..12744
                     /locus_tag="IWO50_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359106.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(12773..13237)
                     /locus_tag="IWO50_002000"
     CDS             complement(12773..13237)
                     /locus_tag="IWO50_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0359107.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(13369..14622)
                     /locus_tag="IWO50_002001"
     CDS             complement(13369..14622)
                     /locus_tag="IWO50_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0359108.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(14651..14968)
                     /locus_tag="IWO50_002002"
     CDS             complement(14651..14968)
                     /locus_tag="IWO50_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0359109.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(14994..15314)
                     /locus_tag="IWO50_002003"
     CDS             complement(14994..15314)
                     /locus_tag="IWO50_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0359110.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(15778..17193)
                     /locus_tag="IWO50_002004"
     CDS             complement(15778..17193)
                     /locus_tag="IWO50_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0359111.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(17210..18526)
                     /locus_tag="IWO50_002005"
     CDS             complement(17210..18526)
                     /locus_tag="IWO50_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0359112.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(18676..19374)
                     /locus_tag="IWO50_002006"
     CDS             complement(18676..19374)
                     /locus_tag="IWO50_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0359113.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(19663..20652)
                     /locus_tag="IWO50_002007"
     CDS             complement(19663..20652)
                     /locus_tag="IWO50_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0359114.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         6547 a         4274 c         3490 g         6445 t
ORIGIN      
        1 ttcttcttct gtcaaaacgg catgactttt ttcagatgct tctgattctc ccgtcaaaat
       61 cgattcttcc actaataaat catgtgcttc gattaatcgg atatctgctg gaacgacatc
      121 tccggcacgt aagacaatga catctccttt taccagttct ttcgcaggga ttttttcttg
      181 catcccgttt gatagtacta tcgcttcttg tcccatcagc tgcttcaaac cattcaatga
      241 ttcttccgct tttctttctt gccagtaact aacgagtcca ttgacgatca ctacgaataa
      301 aataatactt ccttcaatat attcactaga agcaaatttc aaaaaagagg caatgatcaa
      361 cacgatcatc agcagatcag taaaatgctt tgctattttt ttccatcgtt ttgtctgctg
      421 ccttgctttt atttcattat atccttgatc agtcaggcgt ttctgtcgtt cttcttgcga
      481 caagccttct gttgatgttt gatagttttt caatacatct tctaccgtca atctgtacca
      541 gttcatccta tcccctcctg tttaattgat actgttatta tacgcaggga agttttttca
      601 ggcgtatcga ttatttcagg ggggtctgaa ataattcgtt ttgcaaaatg tatctgggcc
      661 taactgatat ttctattgct tattctttta tatacaaaat ttaagaatac aaaccagata
      721 accaataaaa aaggaataaa gcgtgtcgtg ctttcgaata acaagctgaa agcaattgaa
      781 gtaaaaaaca ccaacaaagc aagatcttta tatctttgtg tagtgttccg tttcttttct
      841 ttaataaatt gaacatgtgc acacaagatc gaagaccaca caaatagaaa acaactcgtt
      901 gtcatgcctg ccaaaagctg aaaaaacaat cgactatcag ctacaaaaaa atgtagacaa
      961 agaccggcaa aaaataacat tcctgtaatt aaaataccgt gtaaaggtac gcctttctta
     1021 gaaagcgcac aaaaccaatg gggcaaactt ttttcttttg ctgtttgata taaaatcctg
     1081 ctagtactgt agactgcact atttgctgag gacaaagaag caaccaaaat cattagattg
     1141 acaattgctg ctccgccttg gcaatggatc acttgcagca tagtcactaa aggactttct
     1201 tttaaatcta aacaatgcca tggaacaaca aataaaatcg acaaaatcgt tcctatatag
     1261 aaaaagagaa tacgccaagg cagacagttg attgcttttt tcaaattctt ttgcggatca
     1321 cttgtctcgc caacggttaa gccgatcatt tcaattccag caaaactgaa taaaaccata
     1381 ggaaatgaga ctaccattcc agcaaagcca aacggtagta attcatcgat ctgtccaaaa
     1441 ttttctttga ttccgataga ctgggtatga aaatctgaga cactcaatcc tatacccaat
     1501 aagataaaaa ggaaaatccc caagatcttg atgattgaga aaaaagattc caattcacca
     1561 aaccacttga ctgcaacaag gttgactgcc attaagatca ttagtgcgct acctccgctt
     1621 atcaaagctg gtacatgtac aaaccagaaa tggatataca atcctattgc tgttaattcc
     1681 gcaagtccag ctccaagcca gcaaagccaa taagtccaat tgacaaaaaa tgcccacttt
     1741 ttgcctaaat aatagtcaac gaaatcactg atagagttaa atgtactatt tgctaaaaat
     1801 aaatcaccca tggcccgcat caaaccataa atagccaaac cgcatatcaa ataaacgaga
     1861 ataacagctg gccctgctga tgcaattgcc tttgctgaac ctaaaaataa tcctgttcca
     1921 attgcgccac ctaatgcgat taactggata tttcgattag ttagagtccg tttcattgcc
     1981 ggtttttcca tcaagttcac ctttttcttt ttcaagcagc atggcgactt cctgttccca
     2041 agaaggtttc tttatttttt caatacagag tggtactaca aacaaaatga cactgatggc
     2101 tgtgactgca attagattga tccatgttcc agctttcatc gtcagtccga cagaaaatcc
     2161 gattgccgca aataaaacaa taacgacagt ccaaactcca agattgctct ttttctcttt
     2221 ttttccaatt ctaaatggtc gattcaactt tgcatgtgta taacgcaatc tcaaaactgc
     2281 gatccccatg atgacataga cgatacagta gataacagta gttgaattta cgagcatcaa
     2341 aaatgcttga ttgacgccag gaatcagcaa ataaaaaagt gcaaagattg agataatgac
     2401 agactgagtc aaaataacgg atttggatac gtcaaattga ttcgtcttcc aaaaattgaa
     2461 ttttggtgga tacgcacctt ttctagcact ttctgtcact gtctttgctg gtcctgaggc
     2521 ccaagcagat aattggactg ctacaccaat gaagacaagt aaactaaaca gattcacaat
     2581 aagatgtggc aaaccaagaa tttggcaata aatcgaaata gattgcgcga tattattcaa
     2641 ttccatttgt ccttttggta cgacatttgc tacaactaaa gcattcaaaa tattgacgaa
     2701 aaatataaaa attaacgcgg caaatactcc gcgaatatac gttttaactg gattttccaa
     2761 tcgagtgata tacacagagg acatttcgat accagtaaaa ataaacatga tcggcgcaaa
     2821 ataaacaaat gaggatgcag tcgtagtatc tggtattaat ttgtcccatg aaaaactgcc
     2881 taatgttccg gttggtacga tcccaacttt gatccacgca gcaaacccta ataagagcat
     2941 catcgtcaaa ggcacgtaca atcctaacca aacaccgact tttccgccga tttttgccat
     3001 atcaaatttc aaattcaaaa aagtgatgat ccaataaacg actaaaatga caactaatgt
     3061 aaatttgctg ttcaaaccaa gcggcacatt atcgataata ttaccaaaca acggagcgag
     3121 tgctgaagcg accatcacca tcccaggaaa catttggacc cataacagcc atgagacaac
     3181 gaacccccat ttttttccta gagcattcga tacccaaagt tctggaccac ctttttgggg
     3241 aaacattcca gcaaattcac cagagatcaa tgagattgga aaagcgtaca ataatactgc
     3301 aaccagcatg taaaaaatca tcgtccagct cgttgctgct acatctggaa tatttcgtac
     3361 acttgctaca agggcgcagg tcattccgat aaaggtcatg gtcgtgattt gctttgatga
     3421 accagtatct ttcatcttta ttccttcttt caatagcgac atatgttttt gttttctttt
     3481 atttttgttc aagaacagaa atgacaaggt ttcatcgatt cagtacaaaa cccagtatca
     3541 ccgaaacatc cattgaacga tcttagtgaa ctgggtttga tacatgaaac gaacgatcca
     3601 acttatagaa cgttttataa aaaagaatat cttggcgagg aatatatgaa aaacaggggg
     3661 aaatacaatt gttttgatga agcaaataat tgtctgttct ccatatgtct cttgttattc
     3721 tttcacacaa aaatcagttc tattggatga tttcgtttag tttcttttcc attgctttgg
     3781 cgattttttt tgtataaaaa ttgaaaattc gatcatcatt caaataagga gtcatgatag
     3841 ccgcacgtag cagagtgatt ttctgttctt ttttccattc ttctttcgtt agacccattc
     3901 tttcaacaaa acgaatcgga gaatcaccgt aatcttcttg tgtaaatgta gtatgagaag
     3961 taataaaccg ttcaccataa acatcgccat ccatataaga agaccgatcg aacatttttt
     4021 catttaattc attgatagcg ttcaaatctg tacagccttg ttctttgaat acccaattga
     4081 ccatattaaa atcaggatga ttcaatggat agacttcgat tgtttttcct ttaacagtaa
     4141 aagtcagatg atcgagaaat tctctgaatc tttgagctgc ctcgattgaa gccccaatca
     4201 attgaccata tccggtcaca ttcaatggca atacacgatg tgctgtccag actgcggcgg
     4261 cggttgctcc agcttttgat ccttccaaaa tataagctcc taacatatct ggagccttta
     4321 ctgatttttc aaacacatat ggcgcgaaat aagaaatgat atttctcata tttttatgtt
     4381 tgattacgat tcctccagca gcataaggga catagcccat cttatgagga tctatcgtga
     4441 cagaatccgc ttctgaaata gctctgaacc cttcatatac ctctttgtca atcgttacgc
     4501 agtggtggaa aacatgatgt tcttcaaaga attcagctaa ggaagcatac ggcacaaatt
     4561 ctcctgcttc gtttagaaac agtgaacgcg catagccgcc atatgcggca tcaacatgta
     4621 aatagaaata gatcccttca tcttttaacc tttctcttaa ttggacgatt ttatctacac
     4681 tgtctacttg accttcttct gtcgttccta cgacagcaac cacacctaga attgggattt
     4741 tttgtccagc taattcacga attgtttttt ccaaagcatt aatatccatt cgataatcct
     4801 cttgaactgg gatagcaacc atctgatcta agccgactcc gcaaatatct aacgccttca
     4861 tccaagaata gtgttttgtt tgagggacca gccatttccc taatctttgg atattttttc
     4921 cgctacggga agaagctgct ttgacctcat ccatttcttc gtccgtaaag ctttcggtca
     4981 tttccaaaat ttcttcaaca gataaattca aaagttccca ctcagacatt tttttcactt
     5041 tttcaggcag cacttccttg acagctaaag gaatcgattt gatgcaacgg gcgtaccata
     5101 atccttcaag attagcaata gaaccatctg ctgcaatgtg tccccatccg tctgtcatat
     5161 tgaataatga agcaaaatcc tgtcctactt ctgcttccat ttgcgaagtt gccatagaag
     5221 attccaatgc aacattattt gggttccaca gcatcgcata gttgtaagct aataaagcag
     5281 gcatcaatgt ttcagcattc atttgtcccc agtaacggcc tgctgaatgc catgggatcg
     5341 atcctgcgcg taatcgctga ctgacttcat ccaagacctc tatcatatgg tcgcgggtgg
     5401 ctagataatc tggagtgagc ttatctcctt cgctaattgc aggcatatct gaaggaatat
     5461 aattttctct ccatccaaga tgttcatcta ccatcttgtt caacaacatt ttataaactg
     5521 gtccattttc agctttgtcg cctataaaga cggccttgat atccatatct ttcatttttc
     5581 ttccctccat attttgttgg tcaatgtctt gcagatacat gtgtttatct gtaacacctc
     5641 ttcactatag cgccaaaaat gagaaatttt tttttataac atcaaatcca cgtagatttt
     5701 tacaaacaaa aataaataaa gtcggatttt tctgataatt caacgcaaaa aagcgaacaa
     5761 tttttcaata aatcagtctt ctaaaataga taacctaaaa aaagcgaaca aacaaataaa
     5821 atgactgtcc ttcattcact aaacatattt ttattttttt atattgttaa aaattcacaa
     5881 tttatttttt aatagaatta tctgttttcc aaattttata acataaaaaa ccttttttaa
     5941 cggcatttat atgctttttc agaaattaac tgatgatttc aaaaagaaaa catgtcataa
     6001 tatttcccat attgtgcaaa aaaatacaag gcggaggctc tactatgatt gaagaatata
     6061 ttgagaaaaa tattttaaga cagctgtttt tatgtgggca attctatgta aataaagaag
     6121 tcaatctaga aaagctctcc aatctcctgc atgtttgcaa aaccacttta ctcaatgata
     6181 tcaataacat aaaaaaagaa tttgaagaac agatcgctta tacacatcgg gaaaaggacc
     6241 gttacacttt atatttttca gaacatatac ctcgatgcaa gatcatgcag caattgtctc
     6301 aaaattcgtt gtttttaaaa acttgtctgc tttatttgga agaagatgaa cctgattatc
     6361 ttcaattaac tgaatgtgaa ttcatctctg tcagcaaagc ttattcatta aaaaaacaag
     6421 tattagctta ctttaatgac tgcgggatcg aaatcgaccg ctactcccct cgattcactg
     6481 aaatggaaag acgtctgctg cttcttaatg tgtcgtatcg tttaggcggt tttaattctt
     6541 gggagctacc tgaatcgttt tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa
     6601 atagtggcag attttatgat aaagaaaata aagaaatctt atcgattggt tttgctatca
     6661 gtttcctacg ccagcaagtg tgtgctgtga caatagattc aaaatttatc gaagaaatca
     6721 aaaaacgtcc tatttacaat tatgtcgaat cagcatggga aaacactgat ttccaaacct
     6781 attataaaaa agaagaattc gcctttattt tgacactatt caatctttgt aattatggtt
     6841 tccattctta tcagttgatt gcggaagact ttcaacagct acaccaagtg tttattgaca
     6901 atactcccga aataaaagaa ctagttgcta cctttgaatc tcacttcaat caagaattat
     6961 tcggaaacca accgttcgaa cgtgcgctga ttcatctgat gcgttcggct tgggataact
     7021 atcaattatt catgccagaa aaattttatc ttttaaatga ggaacagaca aatttatata
     7081 aagaagtcca gacaattttt tccagctggt ccagccagtt accttatgat ctacggttga
     7141 atcctaattg catgcgggct tttgtgatcg aattgagcgg aattctccgt ctgacaaaag
     7201 aacatctgac tatctatatc gtcacaaatt cggatgttca ttatctgatt tatcgggaag
     7261 ccttggaagc tgtaactact ttcgattttc aagttgcacc aacaatttat tcaagtatat
     7321 ctgatataaa gaagtatgcg cagcagtcct caaaccgggt attatgtgaa cgtacattat
     7381 atacaccaga tgcggtccaa tatgaaaata ttattcctat ttcgatcaat acgatcgatc
     7441 gagctatcat ttcagctgtg caaaacaaat aaggggctgt gacaaaaatc atgtcacagc
     7501 ctcttatttg ttgaagatgc ccatttctgt aacaatctct tgatacacgt caggctcaca
     7561 acctcactta ttttggacat ctaggggtcc gatctcttta tttaaaaaat aagaaatgat
     7621 ggttcggata actaccaacg ccgctagttg gaaaatatcc tgcaaggtag gtttgataat
     7681 cgattcgatg atcccagctg aaacaagaat ttccaaactt agaagtatat agcctcccaa
     7741 catcttttta atagcattgt tctcgcttac tcggtcgctt tgcacttttg tatggacaaa
     7801 taaatatttc aaaaaatcct tgattgatag acaaacaccc caaaccaata aacagatagc
     7861 aaagatatca agtcccagta caagtttttc gaaatatgga agaagaaact gcatcaactc
     7921 tgtcgtcatc ctacctctcc tcttctttgt ttttctttgt cttctatttc ctgattcaag
     7981 aaataagaaa tgactgttcg aatgataata ataagtgcaa gcttcaaaat atctgtccaa
     8041 gtcggcttga taactgactc gatgatatca gcaacgacta gtacttccaa gcttaacaaa
     8101 atataacttg ctagcagttt tcttgtatct gcattacgag tattacgttc tgcatagttt
     8161 ttcttgacat gagaccaatc aaaaagtttg aatagaacaa gaacgatccc gacaattaag
     8221 ataccgatag aacaaatatt taaaatagca atgatccatt ctagaaattc aattaaattt
     8281 gagcgaaaca tatacgctac ctactttctt ttaatttgtt cctattcttt tattgtaaaa
     8341 gaaagttacc ttgttcgtct atttaaaagt tgttctaatg ataacggttc gcttaaattt
     8401 ttctttttca ctctcccatt tttctccctc catccataaa tcgtggaagt atccaaaaca
     8461 acgtagcaag ttaaaataag gcttagtccg tcatcagctt ttcaaagaag cactgtctcg
     8521 gtgctttttt tgtacccaaa tttctaaaaa tagaaccata cacctcctca aatcttttct
     8581 tcttacctac cctattttta tttacaggaa aatcagacac ttcttctttt ttgtcaaaat
     8641 ttcctttttc ttctcgtcct atcatttggc attctaataa aaacgaacta tccttggata
     8701 tagaaagaga aaacaaggag gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg
     8761 gtttaggttt gcttgccctt ggcgctgcat atctaggaaa aaagacagga ctattcgaag
     8821 atgacagtca tttatatgat gaattcgaat caatctagca tcctatttag ttgaatcact
     8881 acggacatac ttggaagaca ttggaggaat cacaatgaga aaaagtaaag caataatgat
     8941 tggtgccggt attgccaata tggcagcggc tgtttatttg attcaagaag gcaaatggaa
     9001 aggggatcag atcacctttt attcattgga tgatcacggt tcaaatgatg gtgcaccgac
     9061 cgacactgta gtggatgaat attggaataa aaaccatcca ttagaaaacc aaaaaggcta
     9121 tattgctcga ggcggtcgca tgcttaatta ccgtacctat gtagatttga tggatctgtt
     9181 aagtcgaata ccttctgtta ctgagccagg gatgactgct gaagaagata cgagagattt
     9241 tgatgcaaaa caccgaacct ttgacaaagc acgtttgatg gaaggtggca aaggaatcat
     9301 tgatggcgga aaactgggat tcaataataa agaccgtatt cttctgacta aactgattgc
     9361 tatgcctgat tcagaagaag aaaaactaga caatatcact atcgaagaat atttcaaaga
     9421 tgatccacac ttttttgaaa caaacttctg gtttatgtgg gaaacaacat ttgctttcag
     9481 aacacgtagt tctgctcaag aattgcgtcg ctatatgcat caaatgattt atgaattcac
     9541 tcaaatcgaa catctagtgg gcgtcaatcg aacacgttac aatcaatacg aaagtattat
     9601 gcttcctttg atcgagtact tgaaagaaca aggatgcaaa atcattttga accgccgtgt
     9661 cactgactgg gaattcaaag aaacaccgat gcaagatgaa atcactgtga ctggtttgaa
     9721 aattaccaat acactgactc aagaagaaga atacgttgtg gtcgatgaag atacagcagt
     9781 tattttcact aatgggtcca ttacagattc agctacttta ggagattacg aaacaccagc
     9841 tgtagaaaat atggattatg gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt
     9901 ctacaacttg ggaaatcctg acaaattctt tgctgatcga gatgcaagcg aatgggtcag
     9961 ctttaccctg acgacgaaaa accatttgtt gttgaatgaa atcacccgta tcacgactca
    10021 agaaccagga aatgccttga attcatttat ttcaaccaca ccgatcacgc cgttaggcca
    10081 aaaagatgtc aatatgtcta ttgtcgtgca tcatcaaccg cattttacgt ctcagaaacc
    10141 aaatgaaaca gttatttggg gttatttcct ttatccaaga cgacgtggcg aatttgtgga
    10201 taaacagtat attaaaatga atggaaaaga aatgctagaa gaactgattg gacaactttc
    10261 aaaagtagat cctggaccag tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt
    10321 aataaataat atccctgtct atatgcctta tgcttccgcg ttgttcaata atcgtgccaa
    10381 aagcgatcga ccaaaagtca ttcctaaaca ttcgaccaat ctggccttta caggagaatt
    10441 tgtcgagcag ccttatcaga tgatctttac tgaacaaagt gctgtacgtt ctggagaaat
    10501 cgctgctttc cattttgcgg gtattcctat gtcccgtctt gtcaaaacac cgcgttacga
    10561 caaagatatt cctactctag ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg
    10621 tgaaaaagct gccctgcatc aagtaataag aacaaccagg ctaaaacagc ttcttatgtt
    10681 tttgatttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
    10741 ttatgaaaaa gctgccctgc atcaagtaat aagagcagcc aagctaaaac agcttcttat
    10801 gtttttgact tggttagact tattaccgca gatgcaagct tttgaacacc gtttaccaag
    10861 aggttatgaa aaaggcgttt agtttcgagt atgaagaaaa attttgtgaa aatggttcct
    10921 cacgtttttg cataaatttg acttaatacc gagaaactga tcccttctgt cataacctct
    10981 ttttatttat gtctgtttct ttttgaaaac aactattttt tcaacgttta ctctcttgcc
    11041 aatccaacat catctaacgg atacaactat ttcaatgatt accattacac taaaaaatcg
    11101 gaaacacacc tctccgaaca caaatacata agactataga atgtttcatt taagaaaaaa
    11161 agcgtttcat tatcaaaaag gtaagatctc ctttaaaatg aaattatctg tagtactaat
    11221 aatgcagaca attttatgaa tctgaaagga gcatctccaa tgaaagcagt cacttggcaa
    11281 ggaaaacaga aaatggaaat cagaaacgta gatgatccaa aaatattaga accaacagat
    11341 gcaatcatcc gtatcacagc aacagccatt tgcggctccg atctgcatct ttaccatcat
    11401 ggtgaagcag ttttggaaaa agggtatgtt gtaggacacg aaccaatggg aatcgttgaa
    11461 gaagtgggat ctcaagttca aacattgaaa aaagggcaac gtgtggttat cccttttaat
    11521 attagttgtg gtcattgtca tttctgcctg aatcacatgg aaagccaatg tgatgaatca
    11581 aatccagatc agttatatgg cgggcttttc ggatttggca agttgaacgg taatcattgg
    11641 ggtggtcaag cggaatatct aagagttcct tatgcagact ctacttcatt tgttgtacca
    11701 gataatgatc ttcctgatga aaaagtttta tttctatcag atattttgcc cacagcttat
    11761 tggagcgttg aaaacgcagg agtaaaaaaa ggcgatacag ttgtgattct aggttctggt
    11821 ccagtcggtc tattcgctca aaaattcgct gttatggctg gagcagagcg cgtaatcgca
    11881 gtagatccag tacagcaccg tctagataaa gcggctaatt acaatggggt tgaaacttat
    11941 ttcttagaag acacggcaca agctggaaca gaattgtatg aactggctaa aggtggcgca
    12001 gatgtcatta tcgattgtgt aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa
    12061 aatttagtaa gcttacaaag cggcacaatc tctccattac agatggcatc gcaagctgtc
    12121 aaaaaatttg gaacagtcca agtaactggt gtttatatga ctccagcttc ttcttacccg
    12181 ctgcaagaat tttttatgcg ggatatcgct gtcaagcatg gacaggcacc aattatccat
    12241 ctaatgccaa aaatttacgc aatgattgca gaaggactat tcgatcctac acaaatcatt
    12301 actcattcgc tgccactatc agaagcagca caagcatatg agatctttga caaaaaagaa
    12361 gataaaaata taaaagttgt tttgaaacct tgaattcagt aaaaaataca ggtgattttc
    12421 tttatgccca aaatcaaacg aaatacccat gagatcgaaa taggaaaaga agaagatatc
    12481 gaaatacgag gacgtcgctt ccgcttctat ttccaaaatc gttacacatt gatctcactg
    12541 gctgtcgatt tattaaccgg gattttctat atttttggaa gccttgcttc cttaactaaa
    12601 atccccgatc gatatggcac ctatttttat ttggctggtg ctattttctt gacgattcgt
    12661 cctattctca gaatcgtaag gaacattttt atttacgacg aaaaaaaagc aatgaagaga
    12721 acaagcaaaa aaaaagaggc atgagaattt ttcaaatctc atgcctcttt tttcacccta
    12781 tcgtatcact ttgatacaga cgagcggtcg tcccactttc ttccttgacc attgtcaaaa
    12841 atgtccactc gtatcgttca taaaacgtgg tatgatctgt aattaagtaa agtttttcgt
    12901 acccaagttg atgcatttct tttctgattt ccgctaatat ccgcttggct atcccctgct
    12961 tacgcttttc cggttcaaca aacagggcac aaagatttgg tgtcagatca gggcggttat
    13021 ggaagtcgtt ttcaatgacg cccgctcctc cgacgatggt ttctttttca tctaagacca
    13081 cataccattg aggaatactt tccttattag cgattccagt acgaatactt tcctcataca
    13141 caacagcagg aactccccat tttgatgaaa accaattcgc gctttgtttt actaattctg
    13201 ggtgttctct taatttgatc cagttatatg taaccaatgt ttaaacctcc gactaaaata
    13261 aaacgtttac aaaaattata caagtcttta tgaaaattag caatgacaac aatagtgatg
    13321 taaaaaaaaa aacggaaaac aatcacttgt tctccgtttt ttgattaatc aagcatctaa
    13381 agcttctttt cttctttctt ggatcgtagc taccttgacg aacggtagat agatcaaaat
    13441 agaaagaacc aaattgacag cggctagtac accacctgca atactttttg ttgccaatac
    13501 accgccaata attggtggag ttacccatgg aggcataacg gtagctgctg gcactaaacc
    13561 tgtagaagtc gcaaagtaag caacactaac taagaccatt ggtgtcaaaa taaatggaat
    13621 gaacatgatc gggttcaaca cgataggtaa cccaaacatc gttggttcat taatattgaa
    13681 gacacctggc gcaatcgaca agtttgttac aaccatatat ggtttgtttt tacgtcctac
    13741 taaataaata gctaacagta aacctaaagt tgcacctgtt cctcctaagt tgacaaagct
    13801 gtcaaagaaa ggtttgttca cgataaacgg tgcgctatgt ccagctgcga ttgcattaac
    13861 gtttgcttca atggctggcg cattgattgt ttgcatcaaa ggatcgacca tattcgctcc
    13921 gtgtaaacca aagaaccata agaatggtgt gataaatgca agcagtaagg cagatggata
    13981 agagtttgca agtcccataa atggttcttg gaccactgta tagaaagaac ctacgatatc
    14041 atcaacaccg aatgcagcaa aaattgcagc aactaaagca aacgcagaga tagtgatcat
    14101 tgctggtagt aatgaagcaa atgcttttgc aactgccggt ggcacaccat caggcatttt
    14161 gatgatcaat ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa taatcccaac
    14221 aataatagca ataaacaaac cgtttgagct catacctaat gctccgccta aagcgaagaa
    14281 agagcctaaa gaaatcacac ctgcggaaac accgtctttt cggtagccgt tagctaaatg
    14341 ataagcaagc aagaaagcaa tcaagacaga aagaatggca aatgtaccgt tccaagcagc
    14401 agccccgaat cctttccatg attcaccacc aaaaattgag ttcatcaatt cttggaaccc
    14461 gggaatcggt aagttattga tcattactgc taatgctcct aagatcatta acggcatact
    14521 caccataaat gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt ttgatgctac
    14581 aggtataaaa tgtttctcca taaatgcaac aaatttatcc ataaatcttc cctcttttct
    14641 ttactgtaga ttacgcgttc aatcgacgat acagatcaat gatctctttt gctaaatctg
    14701 taaaagctat agatgtcatc agatgatctt gactgtggac cgtcaaaagc gtgacttcaa
    14761 cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt gcttggacta
    14821 aagattcctc tgcatctttg attttttcat ctgctaaagt gaaatcacct tttttcgcag
    14881 cagcaatcgc ttccattgca tcgctttttg catttccgcc aaacatgatc agacccatga
    14941 tggcttctag atttttttga tcttccattt attgtatcct ccgataattt ttattatttg
    15001 tttattagtt tttctgcttg gtcgagtact ttttcaccat tcatcatgcc atagtcagcc
    15061 atattgatta catctaaagg aatgccttta ggtgctagct tttcagcaaa ttgattttgc
    15121 ataaagcgta cttgcggtcc taaaagtaaa acgtctacat ttttagattc tagttgatta
    15181 tccgcatctg aagcagatac agcaaagatg tctgcttcca ttccttttgc ttctgctgcc
    15241 ttttgcattt ttgtgactag gagacttgta ctcattccag ctgaacatac aagcatgatt
    15301 gttttctttt ccattttaaa cacgttcctt tctatactta cacttgtata taagcaagta
    15361 gcgtgccaac tttaaaagcg ctttaaaaag ttgatatcat cacatttaag gtctatacca
    15421 agttgcatac acactaaaaa agaaagcgca cactaaacac acaaattaat ttttattacc
    15481 ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca acttttttca
    15541 aataaacggt attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata
    15601 agatgttgtt tttactttgc tattcttatt acttgatgca ggacagcttt ttcacaacct
    15661 ctcaataaac ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc tggattcaca
    15721 acctctttta tttcaaataa ccagtaaata caatgaacat ctacaaaaaa cttctattta
    15781 tttatagatt tcgtcaggca aatccaattc tctcttatca atcaaagtct gataccacga
    15841 agcagatttt ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa ctaatccata
    15901 tcgattctta aatgcattca ttggtgacac acagtcagtg aatgcccata acatatagcc
    15961 ttcgcaattt gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa
    16021 ttcgatacgg taatcatctt gcacgattcc tttctcatcc atatactgtt cttcatattc
    16081 tcttcccata ccgttttctg ttatcagcca agggatatta tgataatttt cttttagata
    16141 catggctata tcatacatga ttttcggata gatttcccat cccctggaat tattcatttt
    16201 cttcccaggt aaatcaaaga cttcataata tcgttcagga tggaaaggtg tctcttcatt
    16261 ccattgataa cgaggggcgc gaacgcgttt aggataatat tgattgattc caaggaaatc
    16321 tactgtatga tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat aaatcgattg
    16381 tttttgacac aactccagga actcttctgt gtagtatcct tttaccatcg gatcaaaaaa
    16441 tactcgatta taaaacaaat catacatata agcagctttt tggtcttctg gagaattgga
    16501 tcgagcatag accatttcag gatttaaaat acagccaatc cttccaccat aatttccgct
    16561 gcggtaaatc tctactgctt tagccgttgc taaaacttta tgataattcc acatcatcca
    16621 cttttttgtg ttttgttcat gcggccagcg aacagcatcc aaatagcaac gcgtttgggg
    16681 aacgattggt tcgttgaatg taaaccagtt ttttacccta tcaccgtaac gatcaaaggc
    16741 aatttgagca tacttagcat aaaaatcaac tacttttctt gaagaccagc catcatattt
    16801 ttccaaaaga aaagctggga cttcataatg ttccaaacaa atcatcggtt ctacgtctgc
    16861 ttcgatcaaa gagtcgatca tttcacttat atgctgagca tattcttcat ctactataca
    16921 gttctcataa tcagtaaaaa aacgagccca attgatagaa gtccgataat gtgtcagatg
    16981 gatggatttc atcagatcca catcctgctg gtattgttcc ataaaattag ttgctacggc
    17041 tgggccatac ccattatgcc aaacaaaatg ttcattttta taccagctat ctataaacga
    17101 atcctgtcct tctttttttc ctttccaacc ttccgtttgc caagcagaag atgcagcacc
    17161 aagaataaaa ttttttggaa tagtaatttt catgatttcc tcctgttttt tagatcccat
    17221 cattttccac acgatttgct acttttacaa atggcagata gatcaaaata gaaacaataa
    17281 tacaaacaag ttgcgtaata actgcaccga tatttcctgc tgttgccaga taagcattga
    17341 cgatcggcgg cattggccaa gggaccatta caactgcttt cgccgcaaat cctaaagcgg
    17401 ttgcaaaata accaatcgtt ccagtgataa gaggcgttaa gataaacgga atagctaaaa
    17461 ttggattcag catgatcggc ataccaaaaa taactggttc attgatgttg aagatcccag
    17521 gagcaaggga taacttggtg atttgtttca tatcttcccg ttttccaact aacaatactg
    17581 caatcagtaa accgatcgtg actccagacc cgcccatact catatacata tcccaaaatg
    17641 gcatagtaat aatgttcggt acttcttttc cagcttcaaa agcttccgta tttacagcaa
    17701 ttgccgctaa aagcaacggt tcccgtatcg gcttcaccat ttgatttcca tggataccaa
    17761 tcacccaaaa gacttgcgca ataaacatca atagcaaaat acccggtaac ccttgtacga
    17821 caccttctaa tgggcgttgt acgacattgt aaatgatatc ataaaggtac attcctgtaa
    17881 gttctttgat gataaatccc gcagccgcga tacccgatac agtcagaatc gtcgggaata
    17941 aagcagaaaa actagcagaa acattcgttg gcacggtatc aggcattctc agctgtagtt
    18001 tcttctgttt cctcaaccag caataaagtt caatagaaag gatggagacc aacattccca
    18061 aaaataaccc tttcgtatct gtatattgtt tagccaatac gttttctact ataacagtct
    18121 gattttcatt cactaattca taaacagttg gattaacaga taaataagac atcactgcga
    18181 tgataccagg gaatacacca tccactttat tcagcttggc taattcgatc cctatcagaa
    18241 aaacagcata gatggtcaag aaacttagtg tgacatagct gatggcagat gcgataggct
    18301 ttaattcttc taaaaaacgc aagaaactta tctgtgctaa gccattttcc gagtcaaaga
    18361 ccattgctga aaacaatgtg ccaaaagcac ccgtaataat taccggaatc aatgcagcaa
    18421 atgcgttttt tatcaccatg atatacctta aattattgat tctcgttgcc acgttcccta
    18481 aaacattggc taatttatcc ataaaactca ttccagatcc cctcattcct gcttcttctc
    18541 cttgacgtca tctctttttg tctattagtc agcgcttaca tttcgatgaa cagaaactat
    18601 ttactcctct tttttaccaa atataaaaca aaaattcatc tgatgtattt atcataaaat
    18661 acatcagatg aactgtcaat tagtttcttt ttctcctatt attttctgta attctctccg
    18721 atttattgcc atatggacag tcattgcatc aaacgcagca agtgcattcc gcctttcgat
    18781 ggcctgaacg atctctgtat gaatatcgat cgtctcgatt ttcagatgct ggatatcata
    18841 atttttatta aacaaagcaa ttgattcatt aatgattggg ataatatgca gcaaggctac
    18901 gtttccgcta gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa catgttcttg
    18961 tgtgccttct cgaaaagaag tttcaatctt ttgcgttaat tttttcattt ggacgatttg
    19021 attcttcgtt gcatattccg ctgccaaacc tgccatctta ggctccaata aatatctcag
    19081 ttcaaataag tctcgtatca tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc
    19141 attggcaatc cctcgcttat cactgacaaa tgtccccgct ccttgccgaa cttcaaggat
    19201 attcctggaa gctagagccc gcacagcttc tcgcaccgtg cttcgcccca cctctaaata
    19261 atcggacaat tcatattcat tcggcagttt atctcctact tgcaactgca tttcctttaa
    19321 atatctaagg atcttgtcag tagttacttc tactagggtt ttattttctt ccaaaccaca
    19381 tacctccttt tcatctgatg tattttttcg taattattat aacacgtttg aatcatctga
    19441 tgattttttg tatacaaaat tgattaatcc gttcatagac atacaaacga agaagatatt
    19501 taccataaaa aagactagga taaaattcac tcctagcctt cgatcatttt tattttgaac
    19561 gacaaaaaat atgatgttca ataacagacg atcttcattc gattcttgat gtcatctttt
    19621 gtttttttta atatatggat aaaaagtttg attacatcgt ttctattcta gaatttggtc
    19681 gattctcaat tcttcttctg gtgagaaatc taaattatcc atcattcgaa cactttcttg
    19741 taactgactt aaccgacttg ctccgaccag tacacttgta acagattttt gtcgcaaatt
    19801 ccatgcaagg gccatttgcg ctaatgattg tcctcgtgat acggcgattg tttgcagtgc
    19861 tttgattttt tccagagttg atccaacttg ttcttctgaa aggaatggta tttccttacg
    19921 atgtgctcta gaatcttcag gaataccatg tagataacga tttgtcagca acccttgagc
    19981 aagcggactg aacgtgattg cccctagccc ttcttcttct aatactggag atagatcgtc
    20041 ttctagtagt gcacgagaaa acatattgta gcgcatctga tgaataatga acggtgcttc
    20101 ctttcttttt aaaatttcag tcattttttt tgtatcttct ccattatagt tggatatacc
    20161 aatatataat gcttttcctt gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa
    20221 aggtgtatca gggtctgggc gatgatgata gaagatgtca acataatcta gccccattct
    20281 ttggagactt tggtcacaac ttgaaatgat ggatttacga gagccccatt ccccatatgg
    20341 cccttcccac atatgataac ctgctttgct agaaataatc agttcatcac ggtaggcttg
    20401 aaaatcttct ctaaaaatac gcccaaagtt tttctctgca gctccagcag ggcctccata
    20461 attattcgca aggtcgaaat gggtaattcc catgtcgaat gcgcctctta aaatctctct
    20521 ttgattatgg ataggatcat aatcaccaaa attttgccat aatccgagag ataaaatcgg
    20581 taatttcaaa cctgaatgac cggatttacg atattccatt ttttcataac gtgcactatc
    20641 tgcaatatac aatttttctc ctcctcatca aatcttttta aaacgctttc ttttttctat
    20701 ttttagttta ctacatttta aagaaaagga gttaatcaaa atgatttttt cgtata
//
LOCUS       DACEDM010000059        20738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEDM010000059 DACEDM010000000
VERSION     DACEDM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.059"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..237
                     /locus_tag="IWO50_002008"
     CDS             <1..237
                     /locus_tag="IWO50_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0359115.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(661..831)
                     /locus_tag="IWO50_002009"
     CDS             complement(661..831)
                     /locus_tag="IWO50_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0359116.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1025..1204)
                     /locus_tag="IWO50_002010"
     CDS             complement(1025..1204)
                     /locus_tag="IWO50_002010"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359117.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1591..2703
                     /locus_tag="IWO50_002011"
     CDS             1591..2703
                     /locus_tag="IWO50_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0359118.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2781..3074)
                     /locus_tag="IWO50_002012"
     CDS             complement(2781..3074)
                     /locus_tag="IWO50_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0359119.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3242..3556
                     /locus_tag="IWO50_002013"
     CDS             3242..3556
                     /locus_tag="IWO50_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0359120.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3577..5556
                     /locus_tag="IWO50_002014"
     CDS             3577..5556
                     /locus_tag="IWO50_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0359121.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5569..5895
                     /locus_tag="IWO50_002015"
     CDS             5569..5895
                     /locus_tag="IWO50_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0359122.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5911..6429
                     /locus_tag="IWO50_002016"
     CDS             5911..6429
                     /locus_tag="IWO50_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359123.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6429..7781
                     /gene="celB"
                     /locus_tag="IWO50_002017"
     CDS             6429..7781
                     /gene="celB"
                     /locus_tag="IWO50_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0359124.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7865..9370)
                     /locus_tag="IWO50_002018"
     CDS             complement(7865..9370)
                     /locus_tag="IWO50_002018"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0359125.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9462..10052)
                     /locus_tag="IWO50_002019"
     CDS             complement(9462..10052)
                     /locus_tag="IWO50_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0359126.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10231..11877
                     /locus_tag="IWO50_002020"
     CDS             10231..11877
                     /locus_tag="IWO50_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0359127.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11926..12645
                     /locus_tag="IWO50_002021"
     CDS             11926..12645
                     /locus_tag="IWO50_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0359128.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13124..14071
                     /locus_tag="IWO50_002022"
     CDS             13124..14071
                     /locus_tag="IWO50_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0359129.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14083..15003
                     /locus_tag="IWO50_002023"
     CDS             14083..15003
                     /locus_tag="IWO50_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0359130.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15044..16501
                     /locus_tag="IWO50_002024"
     CDS             15044..16501
                     /locus_tag="IWO50_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0359131.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16846..17460
                     /locus_tag="IWO50_002025"
     CDS             16846..17460
                     /locus_tag="IWO50_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0359132.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17453..19177
                     /locus_tag="IWO50_002026"
     CDS             17453..19177
                     /locus_tag="IWO50_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0359133.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19174..20625
                     /locus_tag="IWO50_002027"
     CDS             19174..20625
                     /locus_tag="IWO50_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0359134.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6690 a         3497 c         4147 g         6404 t
ORIGIN      
        1 gatgcgacac cagtaacttt caagatcgaa aaagaacaag aagtggcgat ctttttaaca
       61 aaagagaata gaaaagttgc tgatagctca gatataagag gaaacaatcc aacaagcagt
      121 ggaaataagc atcttccaaa aactggagaa accatcatta gccagtttat attgtcaata
      181 ttgggtgtgc tacttgtttt catcagtata aaatttagaa agaaacgaaa tatatgagct
      241 aaaataaaaa attgaataag catagttttt aaaatcaact agtacccatc aattgaatgg
      301 aaactaccga ttttagaagg ggtatacaaa caaatattag tatcggagta gagaaggttg
      361 aacccaacca gtaaataagt aaagacaatt ttgtgctgcg ttgagaagtg aaaaataaac
      421 ctttgtagta atactagtta ataaaaaata tacaaaaaca ctgcgaaaat cattcttgag
      481 tttcgcagtg ttttttgcct aatgactcaa gtttattggg taatactttg attttctttc
      541 tacaaataat cagcgatcag ttgtaatttt ttctctagtt ttaatggctc tttgtcaata
      601 aggagaaaaa caagtcgggt cttttgtctc ggaaagcttc aaccaactcg tgaataaagg
      661 ttacgcctcc ttgaaaggcg tggaaaaacc aagtaatcga tcaatcacca atttcgcatt
      721 tggtagaaca tgtttggtga gctgaaagta ggtggcgttc atgtctgtaa ctaagaattc
      781 tatttcttta ggattagtgc agctaaggaa atagtttgtt aatcgaggaa ttttttgtgt
      841 aggtaaattt tctcgattaa tttttctgtc tcgccatccg cggaaataaa actcagttta
      901 tcctcaacgg aaacgtgaga acgaaattca caaccctcaa cactcaagac agaattcttt
      961 tggaagactt tggtaaatag cttctcaact ctttcaatgt gcgaataacg gttgtcaaag
     1021 atacctaaca acttttcgaa ataaaaaata acgacacttt ttcagtcagt aaagaagtga
     1081 ttttatgtct gacatgattt gaaatcgagt gccttggacg gataaaataa ctttgagcgt
     1141 tccaatggct tcgacagttt ttacaggtat agcgctgctt ttttaggcgt ataaccattg
     1201 gcatatgatt atattattca aagtggacaa tggtttcctt cttcttgttt ttcaccacca
     1261 ttaatttcct atttccatct accacagcag aaccacaatt tctacagaca catggagcag
     1321 ggaagagaac tgcagcaacg atcaatgttt ttttcttctg taaggtttcg taagagacct
     1381 ctgtaatcat caaatttttg tctattgatc tcagaatttt tttgatagaa ccattcataa
     1441 agcatatcgt tctctcagtt gtttattttg tggtgattta atcatacaag aaaacgatat
     1501 gtttttttat aaaaacatga ataggaaaaa tagttgacaa gaaaatgaca acgccatatg
     1561 atgttccata gtatttacaa cctgtcatag atgactgtaa aaaataaaag taaaaagaaa
     1621 ggacgacaac aggtggattt ttatcagtct ctacaactag atccgtttat tttaaaacaa
     1681 aaaatacgag aagcagcttc taaaaaagaa aagtgcatgt atatttgttc attgttttta
     1741 cgatcattat tcatcgtatt gtttgccatt tgttttatca ttataattac aacattattt
     1801 gaatcgaagc ataaaccata cgctgtggtt ttattctgta tgttgatgag tattcgtttt
     1861 gttgactttg gttataagat ttcccattcg atcataggtt tagcaatcgt catgttctca
     1921 ctattagtag caccgtatgt ccagctgatc aaatggtcag ttatgggcat gttgattcat
     1981 tttattttac taagcagcat attaatggca accgctagtg atcccaaaat ggggaatgct
     2041 tctttgtacg gcttttcata tctctttatt gtttattcgt tacctaaaga ttcgttgaac
     2101 aagaatttct ttacacaaac aagtagctta ttatttttgt ttttttgttg gttttcagtt
     2161 ctcctttaca gaaaacatcg agaaaagaac aaagacaaaa gcttgtttaa agagattttt
     2221 ttgaaaggca tgtattcgca ggaaaaaatt tggatgctca tctatgcatt tggcattagt
     2281 cttttgatcg ttgcaggaga gtatgtccct tttcagcgat tgatgtgggc agggtttgca
     2341 ttttcttcta ttgtatctag ctatggattg atgtcgattg gtttcaaaga acgagcagta
     2401 gaccgaatca ttggctcatt gattggatgt gttttattta ttggtatcag ccaatttatt
     2461 ccttttaact gggtcggtat attaggtggt ctagctttag gtgtttgttc tacctataga
     2521 tataaaacag tttttaattc ttttggtgct ttggcaatca ctgccagttt attcggggtt
     2581 ccaggagcag taacgatccg catttttgag aatatccttg gggtatgttt aggaatcatg
     2641 tatattgggg tgactgaaat acttatccga aaaataagag aaaaacacgg tttgaatcat
     2701 tagatgagat cgaataaaaa ataaggaaaa agtgaagata cacatggatg ataaattgct
     2761 atcattccat gtgtatcttt ttacggtaaa tcagtgaaac gttcgacttt catttttaat
     2821 ttatactttt tacggagttc gtttatttga gccatcattt cggatttgtg atgcttttcg
     2881 attgcttctt catctttcca acggtcgatc agtaaaagag attctggatc ttctagtgga
     2941 aagtaatagt catatttctc gtttccttct tctgcacgaa tcgctttgac aatgtttctt
     3001 tcgaccattt cttctgcaaa ttttctggct tgtccatttg tacttgtata caaaatattc
     3061 actgtgatac tcactaaatc ccctcctgaa aaaattctat cactagtata tcacggtgcc
     3121 agaaattatt attcaagata tgatacacat tttttccgca atgctacgga aatatatatt
     3181 ttgaatagaa aacgcttaca agatatgctt ggtttataaa aataaaagga gtgattgatt
     3241 tatgaaaaaa gcattgatta tttgtgcggc aggaatgtca tcctcgatga tggcctcaaa
     3301 gacaacggat ttctttaagc aaaaaagaga agaggtactc gtagatgcag tttcagctac
     3361 agaaggcgat aatatgatta agaatagtga ttttgattta tttctgatca gcccgcaaac
     3421 gacgatgttt ttggataaat tcaaaaaatt aggagaatct gttggaaagc ctgttgttag
     3481 tatccctttc caagcgtatg tcccaatccc tacaggcatc cagaagttag cagaggtgat
     3541 cgaagagaac atatgatgga ataggagtgg atgataatga aacagaagga aaaattgctg
     3601 ttgagatatt tgattgatca caagaaggag tatgtcacca gtcaacgttt agcttctgaa
     3661 ttgttattat ctgatcggac aatccgtaat tacttgcagc ggatcaaaga gttagtagaa
     3721 aaaaacggag gtaagatcat tgctaaacca ggttatggct accaactgca tattttacaa
     3781 agactgacat ttgacttgtt tttgagtcgg caagaaatag tacctggata tgctagagaa
     3841 ccgcaaggat tttatgagtc cgaagatcga caaaagtata tattgaacca attgcttttg
     3901 gaagatgcca ttctttttat ggatgatttg gcagaagagc tttatatcag tcgttccagt
     3961 ttgacaaagg acatgcaaga aatcaaggag cgattgattc catattcact aaaaatcgtt
     4021 tctaagcatg gtcaagggac atggatcgaa ggggaagaaa gaaatcgacg gcattttatc
     4081 atggatactt tttttggaaa ccattatgga aattcattga aagaatattt agggcaaagc
     4141 cgttttttcc cagacatcag ttttgaagag ctagtcatta tcattctcga tgaaacaaga
     4201 gaagctaagt taaaagtttc ggattttatc atacagaatc tgactttaca cttggctttg
     4261 gggatcaaac ggctccgaga agggtttgaa atcaaggaat taggcatcga acaagaagtt
     4321 tctaagcgag tagagtatca agtggcgcag cggatcgttc aacgtattga ggtaatcgca
     4381 ggtgtatcgt ttcccgaaga agaaattgct tatttgacat tacatctcat ggctaagtcg
     4441 aatcacaaag aaaatcaaaa tgatcaggaa ctatcaaagg aattagcgaa agttctgaaa
     4501 agattgtctc aggaatttca tttttcatta gtagaggatc tccagctttt caatggacta
     4561 ctcgatcatt tgtcaccgat gatgattcga ttgagccgag gtattcgcat tgagaaccca
     4621 ttgactgagg agataaagaa agaaaatccc aaagtctttg acgcagtaaa aaggcatttc
     4681 agcaacatgc cggctttgaa aaactatacc atcaatgaag atgaatgggc ctatttggct
     4741 ttgcatctca tggcggcatt ggaaaaagaa agagctgcgc ataaactaca tgcattgatt
     4801 atctgtgcaa cgggttacgg cagtgcccaa ctattaaaga atcgcgtagt cagtgaattc
     4861 ggtaaaaata ttacagtggt aagtgtaaaa ggctattatg agatcaatga aacaacttta
     4921 aaaggaatcg acctgattat ttcttctatt gatctttcga caatgttttt caaaattcca
     4981 gttttacatg tcagtatttt tttgaatgaa gatgatgttc gtaaaatacg aaaggtagtt
     5041 ggagaatcta taccctatca ttcctcagat agaccgatac cagcattttc tctacagcaa
     5101 aaaaagcaga tttacaccga acagttgtct gaaaaatttt tcaagactta tacgtacgaa
     5161 ccgaaaaagg aagaaattct tcatgatttg ctgcaaaagc tgagcataaa tgaagaagaa
     5221 ggatatataa gagaaatgcg aaagcagatc cagcaacgtg aaaatatggg acaaatcata
     5281 ttcagtgata cagtggcagt cccacatccc gctgttccag ttggaatcga aacaaaggta
     5341 gccgttgcat tgattccttc tgggatgcag tgggatcaat atcaagaaat tcattttgtt
     5401 tttttagttt cgccttctta tgtcgaaaat gaaggaatta cggtcgtgac taaagcaatc
     5461 gttaggttag ttgaccagct aaaagtacag caggaaatct tagctgaacc tatttttgag
     5521 aatttcagca gtaaatttat caaactgatc gaataaggag gaaaacagat ggctgaaaaa
     5581 ataaacagtg aaacactaca agtgacttct tttgatatca ttttccatag cggaaatgca
     5641 cggacactga tacatgaagc atttcgctcg atgcgtaaag agatgtttga agaagtagaa
     5701 gagaagttgc gacaagcaaa tgaagagatt cttcatgcgc atcagtcaca aactcaactt
     5761 ctgaaagaat atgccagcgg gacaaaaata gaaatggaaa ttatcatggt ccatgcacaa
     5821 gatcatctga tgacgacgat gacattattg gaagttgcac aggaaatgaa ttatttgtat
     5881 caaaaaaata aatagaaagg aaggatcatt gtgcaggata cagtaaaaag gaaaatcgaa
     5941 ttagaaaaaa agcaacttag tttgaaaaac atgtatttca atcgttatct aatcgtacgt
     6001 tacctgactg cctttttctt tttcatgaac atgcaatgga tgattctgct tctatcagct
     6061 aaaagtttag gaagtagttt accgatgatt ctgattttag ctattttgcc tgcagtagga
     6121 gaacaagtaa agctttatcg aaaacaccaa acgaacgttc catggacaaa gtggtatttt
     6181 ttgttccaga gtgtctttaa tttattagtg attccagtgt tgtttacctc aggatttaca
     6241 ttcttgtatc cttttatggc aaataacaat ggaggacagt tatttgtttt cattcttgtt
     6301 gtaagcggta tttttgccag tgcatttata cagtatcgtt taaagaaaat ttcactcaac
     6361 caagaccaac agtatatccg tattaaacaa tatgaaaaag cgttatattt aggaaaggag
     6421 aataactaat gtttgatttt ttgcagaaat acctgatggg acctatgggg aaaattgcgc
     6481 aacttaaaat cgttcgggct gtcatggcag cggggatggc ttctattcct ttcacgattg
     6541 taggctcgat gtttttggtt ttaaatattt tgccattacc gttcccattt ttagaaggat
     6601 tttttaatgc aacttttttc aaagttagtg acttgtacat gatcgtcaat acaatgacga
     6661 tggggatttt atctatttac tttgccattg tgtttgctta tgaattaacg tcaattgaac
     6721 gagatgaaca aaaattaaat gttaatccgc tcacgggggc attactatct gtttttgcat
     6781 tctttatgtg tatccctgaa ttgatcatga ctgatggtaa aatcagcttg attgcttcta
     6841 tcaatgagac agaaactgta gtaaatggta ttaggatggg ctcttttgtc gaacgtttag
     6901 gaacttcagg aatctttaca gctattatta tgtcgattat tgcggtcgaa ttgtattgta
     6961 tgtgtgtcaa gagaaattgg gtcataaaaa tgccagatgt tgttccgcca ggagtttctc
     7021 gttctttcac tgctctgatc ccaacatttg tcattgcgtt tgtggtgatg atcatcaatg
     7081 gattattagt tgcactagga acggatatct tcaaaatgat tgctatccca tttgggtttg
     7141 ttacgcagtt gacgaataca tggcttggtg tcatggtgat ttatttcttg atccatgcat
     7201 tgtggatcgt agggatccat ggagcgaata tcatcacttc tttcttgacc ccgatcgtat
     7261 tagctaatat ggcagctaac gcacaaggcg caaattatcc gttggcagga gaattcaaca
     7321 attcttatgt aaccgttggt ggatcaggtg cgactttagg attgatcatc tttattgcat
     7381 tcctcgcgaa atctgatcag ctgaaagtgt taggaaaagc ctcacttgta ccaggattat
     7441 tcaatatcaa tgaaccaatc attttcggga tgccaatcgt ctacaatccc tatttagcgg
     7501 ttccgttttt cttagctccg atggcttctg ctacactcgc ttattttgcc attcggctgg
     7561 aaatcgtcaa accgatgctt gcacagatgc cgtggccttc accagtcggg atcggagcat
     7621 ttgttggtag tggtggtgat tggaaagctg ctgtgttagc agtcatttgt gcaatcatcg
     7681 cattcattat ctggttccca tttatcaaat tctatgataa taaattatta aatgaagaaa
     7741 gagaaaaggc agcagaatta gctagtgaaa agttaaaatg atttctaaga aaaaaggaac
     7801 tgtgacaaaa aatcacagtc cctttttttc ttagaaatgg gaaaccacta gtatataaat
     7861 accattattc ttcaatcaac cgcttcacga ttgcataatc cccttcataa ggttcatcta
     7921 caccattgat tttttgatag ggatccacgc cttttcccgc aataacgact gaatcttgcg
     7981 gagcgctttc tgctaaagcg cgtcgaatcg cttcaggacg atccatttca taatgaacga
     8041 tcaaatctgg attagtaatt gcttcattga tttcttccgc gatttttttc ggatcttcaa
     8101 atgctgggtc atctgccgtc aaaaaggcga tgtcagctgt ttctgatagc acttttccaa
     8161 aatcatgtcg acgggagatt gctttgtttc ctgggctgcc taatacaaca attacacggc
     8221 catctggatg ttcatttttg gcaaatttca gcaatgtttt caagcttaga taattatgtg
     8281 catagtccac atagacatgc gctccattgg attggatcaa ttgatccatt cggcctggta
     8341 cacgagcttc ttttaatccg tcttgtgcat cttgactgga ggcacctgtt agtgctgcgg
     8401 caatgatcgc actggctgca ttttcttggt tgaatcctcc agccagcaaa atatcataag
     8461 cagtatcgca gatagctaag cgatcttcat gagaagataa agtaaaaccg tgagtacctt
     8521 tatctgaacg gatcacttga taattgcttt cttccgaacg accataagtg atcgttggaa
     8581 tctggaatag atcggctgtt tccctaagta aatcaaaata gtcactttca tgatttaaaa
     8641 caactgtttt tgaattcaag atcaattgtc gcttgcaata gaaatagtca tcaaatgtcg
     8701 gatgttcgat cggactgata tggtcaggcg agatattcaa gaagattccg acatctaaag
     8761 tcaagccgta tacgcgttgt gttttgtaag cttgtgaaga aacttccatc actagatgag
     8821 tcataccatt ttcaactgct tctgacatca tacgatataa atctaatgac tcaggcgttg
     8881 tcaaatggga tttgaaatat gttttgccat ctaacgttgt attcatagta gaaagcaatg
     8941 ctgttttttt atttgttgtg tgatccaaga ttgcttttgt aaagtacgca gcagttgttt
     9001 taccttttgt tccagtgaat ccgatcactt tcaatttatt ttgcggatag tcgtaaaaag
     9061 ccatagcaag aagcgccatt gctttacgga tatccgttac gatgattcct actgctgaat
     9121 tttcgtatgg ttgttctgat acataatatt gaataccctg ttcgattgct gaatccaaat
     9181 attcttcttt gaaattcatt cctttacaga aaaacaaagt agaagcatca gcttttcttg
     9241 aatcataact taactgaaga aattcagctt tttctggtac atcaagatgc cagcctcttg
     9301 ttgaaacaaa ttcttttaat agattttctt ttaatagtaa atgacggatg tcttctaaag
     9361 agaagctcat tgattgatcc ctcgctttat tcaatttctt ggtttattat agcacactct
     9421 ttttaatata tcgtttgttt ccgctatgac aaaatccagc tttattcaaa ttttcttgac
     9481 acaggggaca agcggatgag tattttcata aacaatgaac tgactgtaat agataggaga
     9541 acgaatagtg tctccaacaa tccaaaaaca taataattca aaaagacgat aagactattg
     9601 gtaagtaaaa gtccttgcgg cattggaagc cgaaaaaatt tgtggaaaat aatcggcggt
     9661 atcgttatac cgccatttga ggccccgact gtgtacaatg tgtaaacccc aagactgaac
     9721 aaaagacttc ctgaaatcag tgaaaagaaa tgtgaggaga tcatttccac ttttggaaca
     9781 atctctaaaa caacaggaaa aagcagactt cccgctacag tcttaaaaaa tactgatttt
     9841 cctaatagga cagcggctag aagcagcatc ataagattga tgctccaaac agtaactgag
     9901 cgttggatag aaaaggccgt ttcaactaaa tgtccgatgg cgcccactcc ggcggaagct
     9961 atatcgtgag ggagcaagaa ccaattcaat ccaactgtga taaagaaaag acctaaaaat
    10021 ataacgggta gtttttttat ttcttgtgtc attgatatct cctataaatg ttagccataa
    10081 aaggctgtat atttctttac tttaccaaaa aaacgaaacc gaattcaaaa aaacaattgt
    10141 tcttataaaa gttatataaa atcgattgcc agtttctgtt ttaccaatta aaatgatagt
    10201 aatgttttgt agaaagttgg aatctgaccg atggataatc aaagaaaacc agcattagat
    10261 caattaacac cgcaagaaaa aatggcacga ggctctgctt ggatgaccgc cagtaatatc
    10321 atttcaagat tgctaggcgc tgtctatatc attccctggt atgcttggat gggcgaaaat
    10381 gctaaggctg ccaatggatt attcaatatg ggatacaata tatatgcatt gtttctactg
    10441 atctctacag caggaatacc tgctgcgatt gcaaaacaaa ctgcacgcta taattcattg
    10501 aatgaatatg gcacttcacg acgattattt atccgtgcat tacagatgat gggcgggtta
    10561 ggcctgttgt ttgctttgtt catgtacatc gcctctcctt ggctagctca tgcttctggt
    10621 ggcggagaag aactgattcc aacgatgcgt tctttgagta tcgctgtatt ggtttttcct
    10681 tgtatgagtg tgatacgagg gtatttgcaa ggcaatcaag aaatgatgcc ttatgcgctg
    10741 tcgcagattg tagaacaggt ggcacgtatt ttttatatgc tattggctac attcatcatc
    10801 atgaaagtca tggatggtga ctatgtgacg gcggtcacgc aatcaacttt cgcggcattt
    10861 atcggtatgc tagcaagtat tcttgtcctt ttgtattttt tgaagaaaca tcaagcgtat
    10921 acatcggcgt ttattcacta cagcgaaaac aaaataaata ttgcaacgaa ggaattgttg
    10981 ttagataccg ttaaagaagc gattccgttt attattgtgg gttcaggggt aacgatcttc
    11041 aaattagtcg atcagttcac ttttatgaag atcatgtctg catccaccga gtattccaat
    11101 gcacagttac tggatctttt ttcgattttt agtgccaatc cagataagtt gacgatggta
    11161 gttattgcat tggcaacatc gatcgcgtct actgggttac cgttgattac agaagctgtt
    11221 acgttgaaag atcgagtagg tctggcaaaa ttgacaagcg gaaatttaca gttgttttca
    11281 ttttttatgt ttccggcgac atttggtgtt atgttacttg cctatccgct aaatacatta
    11341 ttttatacac cggattctct aggaagtaat gtactgatcc aagcaagttt tgtaggactc
    11401 ttcctaggac tatatatgct agtttccaac atgctgcaag gaatgtttga aaacaaggcg
    11461 gcaattaatt atttggttat tgggtttcta gtgaaactcc ttcttcaata cccagccatt
    11521 cgtttatttg aagtatacgg ccctttattg gcgacaatga tcggatttgc tgtgtcttgt
    11581 accttgatct taaagagaat ccatgaagta gcccgcttta aaccaagttt tgtctggcgc
    11641 aggacattgc tgatctttat tcttaccttg attatgcttt tggcttcgtg gatcacaaag
    11701 atgatttttg gtgctttttt aaacgaagat agcaaattcc agtctctatt gttgattctt
    11761 gtagtagcag gtataggggc tttagtgtat ggttatttag ctttgaaaat ccgcttggca
    11821 gaaagactac taggaccagg aatagcgcgt ttacgacata aattaaaaat aaaataagtg
    11881 aatcagaaga gctagattga tgctcttttg aaatggggga aacgcatgcg tttagataag
    11941 tttttagcag atatgggact tggcagccgc aaagaagtca aaggattaat caaaaaagga
    12001 acgatcgaag tcaatggaca agtggtgaaa agcgataaag cgcaagtaaa tgaattcgaa
    12061 gatgcggtta cttatttagg agaaatcatt acgtatcaaa aagattttta ctacattttg
    12121 cataaacctg caggagtcat ttctgctact caggataatc atgatcaaac cgtaatcgat
    12181 ttattggaag accaggatta tcgggaagat ctttttccag tcggccgatt agataaagac
    12241 acagagggcc tcttgatttt aacgaatgac ggggtgtttg cccatcagtt actatcacct
    12301 aaaaaacatg tggagaaaga atatctagct gaggttagcg gtataatgac agaagaagat
    12361 atccgtttat ttgcagaagg actgattatt gatggcgatg agcagacatt gccggcaaaa
    12421 ctccaaattg atcaagtaga tcaagagaca gaaacttcaa ttatccgtct gattttgcat
    12481 gaaggaaagt ttcaccaagt taagcgaatg gtcaaagcag taggaaaaga agtcttatat
    12541 ttaaaaagaa tccgtatggg aaaattttgg ctgcctaaag atttgaataa agggcaatat
    12601 cgacccatga ccaaagaaga gctagaacaa gtgagagaaa aataattgaa tggatagcta
    12661 aaagaataag cagagtagtt gtagcgccat ctgaaaagca gacttgtccg tctgactttc
    12721 ggatggcttt tttgtgaaat ttcggcttat ttccgtagaa gctacttctg gaacaccgtt
    12781 tgttcggaat aagggactgt gacaagactt ttgttacagt ccccattgtt ccttgtgact
    12841 cagagttaga tagtaaggac acgatctccc taatactcag gccaaaacgc tgaattcaca
    12901 acctctgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    12961 tatgaggaac tattttcccg tttttttctc taatactcag gccaaaacgc tgatttcaca
    13021 acctctccta tactttgttt cattttccct atactttgtt tatttattct cattattgaa
    13081 agcgctataa ttacgttgcg aatgaatcga aaggggtttt tagatgaaaa aacgaaaggg
    13141 gttcaatcat ttttttatga atctatggcg ttataaagca cttgtcctga tggctgtacc
    13201 gggtatgctt tggatgattt ttttctttta catacctgta atggcgaatg tcgtagcatt
    13261 caaaaatttt catatttcag cagatgggtt cttggctagc ttgagggaaa gtccttgggt
    13321 gggttgggaa aatttcaaat ttttattttc ttcggatcaa gcatttctca tcactaaaaa
    13381 tacaatttta tataatgtag catttatcct tttgaatctg ctgatctcag tcgtatttgc
    13441 aatcattatg agcgagttga gaaataaacg actcgttaaa gtttatcaaa cgatgtcctt
    13501 actaccttat tttctatctt gggtcattat cagttacttt gtttacgctt ttttaagtcc
    13561 tgacaaagga atattcaatc agtggatcac tggacatgga ggagaagctg tcaattggta
    13621 taatgaaccg aaatattggc cgtttatctt ggtgttcatt ggtacatgga aagggattgg
    13681 ttataacagc attatttact ttgcatctgt tatgggaatc gatccgactt attatgaagc
    13741 tgctatggtt gatggagcta gcaaatggca gcagatcaaa catgtgacga taccacaact
    13801 tattccacta atgacgattt taacgatttt agccgtagga aacattttcc gagctgattt
    13861 tggcctgttt tataacattc ctaggaattc cggtgcatta tatgatgtga ctcaagtttt
    13921 ggatacgtat atctacaatg gcctgacatc cacaggagat tttggtatga cggctgctgc
    13981 aggactttac caatcagttg tcggctttgt cttgttgatg atcacaaata cgatcgcccg
    14041 acgttttgat agcgattctg cattattcta aggaggaaaa aaatgaaaaa aaagaaagtg
    14101 gagagagtcc aagtccgctc tttcaataag acggctaatt ttttctctaa tataatgatt
    14161 gcactctttg ctatttcctg tattttgcca tttatatttg ttatcgccat ctcttttacc
    14221 gatgagtcca tattgtcgac tcacgggtac agtttttggc cagaaaagtt cagtatattt
    14281 ggttatacgt acttgttcga ccagatgcag gataaaattt tccaagcgct tttcgtcact
    14341 gtacttgtta ctgttttagg tacactgatc aatagcacgg ctacttcttt atatgcttat
    14401 gcgatttcac ggtcaaactt tccattccgt cgttttttta ccgttttttg tttgattacg
    14461 atgttgttct caccagggat ggtggccaat tatcttgtta tgacaaatct tcttcagtta
    14521 aaagatacga tttgggcatt gatcttgcca atggctgtca gtcccttcaa tattattgtg
    14581 atgcgcacat tctttaaacg ttcggtctct gattcgatta ttgaatcggc acggatcgat
    14641 ggagctagcg aactgcgaat atttgttcag atcgttttac cgttagctgt tccaggtatt
    14701 gcgacaatca gtttatttgc cgcattgggt tactggaatg actggttcaa tgctttacta
    14761 tacatccaag atgataagct agttccacta cagtatcttt tgatgaaaat ccaaagtaat
    14821 attcaatatc tgacacaaaa ttcaggagca ggaagccaat tgacaggcgg attagcctca
    14881 gttccaggag aatctgccag aatggcgatc gtagtcatct cgacgttgcc tattgcgatc
    14941 agctatccat ttttccagaa gcattttgtc aaaggtctaa caatcggcgg agtaaaagaa
    15001 taaagaaaca aaaatataaa tcataaaatt aggaggaaga aaaatgaagt tatggaagaa
    15061 actagccttt acaggtgttt cagcgttgtt gttgggaaca ttagcagcat gcggaggttc
    15121 tggttctaaa gaccaggcag aagctagcaa ttcggacact ttgcagatgt atcaaatcgg
    15181 agataaacca gataatttcg atcaattgat ggaagtagca aacaaacgaa tcgaaaaaga
    15241 gatcggtgta aaagtcaata tcaattacat tggctggggc gattacgaaa agaaaatgaa
    15301 cgtcatcatt tcttccgggg aaaattatga tatcgccttt gcgaacaact atgtttccaa
    15361 tgcccaaaaa ggagcctttg ctgatttgac tgaattagcg cctaagtacg ctgaaaaagc
    15421 ttataatgat ttagatgaag catatattaa aggaaatctg gtcaatggaa aattatatgc
    15481 tttcccagtc aatggaaatg tctttgctca acaagtactt acttttaaca aagctttact
    15541 agataaatac gatctttcaa ttgatggaat ccaatcttac gctgatgcag aaaaagtctt
    15601 gaaggaattc catgaaaaag aaccaaacac ggcagctttt gccattggac aaggcttcaa
    15661 agtccaaggg aactttgatt atcctttagg gaatacgtta ccatttgcta ttgatctgga
    15721 tggagatgat tcgaaaatca tcaaccaata cgataatgaa acgtatatcg gactgttacg
    15781 tacaatgcat agctggtaca aacaaggact cattccaagt gacgctgcaa cgagcaataa
    15841 ggattatcca ctagaaggaa atacttggct aatgcgtgga gaaacacaag gaccgtatga
    15901 ttatggcgat acaattttga caaatgcagc aaaacaagaa cttgtctcaa aagcaattac
    15961 tgttccacta aaatctacag ctcaagcaca aatggcaaat tttgtcgtat caaatacttc
    16021 aaaaaataaa gaaaaatcag tagaacttct agggctgctt aacagtgatc cagaattatt
    16081 gaatgggctt gtttggggga tcgagggaga agcatgggaa aaagtcgatg gttcagatga
    16141 taaaatcaaa ttgctagatg gttatcagcc aaatacacat atgtctgctt ggaatacagg
    16201 aaacaacaag attctatata cacaagagtc tattacggat gacatgattg ccaaacgtga
    16261 tcaatcgatt gctgatgcag aaacttcacc gattttagga ttcagcttca acactgacag
    16321 tgtcaaaaca gaattaagta atatttctaa tgtcatgaat caatatttag atggattgaa
    16381 tacagggact gttgatccag atgaaacatt accgaaattg aaagacgctt tggacaaggc
    16441 tggctatgat aaggtattga aagaaatgca aaaacaatat gatgagttcc gacaagaata
    16501 ataatgaata aagacaaagg ggttgtgaca aaagacgtgt cacaacctct tgttccgaat
    16561 aaacggtgtt caaaagccag tttctcggta ttaagctaaa tatatgtaaa aatgtggaaa
    16621 gccatattca caaaattttt cttactactc gaaactaagc ggctatttca caaactcttg
    16681 gtaaacggtg ttcaatcagc taaccctcgg tattcgctca caaaacgaca aaatatgagg
    16741 aactattttt ccgtttgttt ctctaataat caggtcaagg tgctgattgc ccaacctttt
    16801 tttgtataat gagaaagtat aaacttaatg tttggaggag aagatatgtt agggaaggtt
    16861 ttggaagagt tatttatccg gatctgggtc attataaaac taaccttgta tttctggata
    16921 tatacatttg ccggcgggat catctttgga ttaggtgcgg catggaaaac agtcaatgaa
    16981 ctattttatt tgtacggatt cgagtataaa gaaatcacaa taaaaagagg ctggaatata
    17041 tataaaagaa attttttaag aggcaatctg ctatttagtt tatttttatc tggtactgct
    17101 ttattgagtt acaatttata tttatctgtt caaatcaaag ggctcatttt tttagctatt
    17161 gattttattt tactttttgc cttgctctgc ctatttgcta cttatcaata cagtatgatt
    17221 ttagatagtg aatatacgat ctctgttcct aatctgctaa agctaagttt tatttcggtt
    17281 ttttccagct tttcgtcttt tctaaaaata attattggta gcggtataat tttaggcgtg
    17341 acttggcaat tcaaaggatt gatcctgttt ggtgtcattg gtcttttgac ggtttggaat
    17401 ggtacgatga caacacattg gcgtgaagaa ttagataaac agttggaaag ttatgaataa
    17461 gtttcggatt ctattttcaa aaggaacctt actaaatcgt ctgttgcgga aatacgcggt
    17521 tatcatgatc ggtgtaacga tgactgctac cgttattttt agtatccata cttgggaaca
    17581 aaatcaaaaa caggcagaaa acatgacaag tgatgccgtt caaagcacaa gcagaatgct
    17641 gaatgataaa acaaccttga gtaggatcat caaaaaccag cttgtaggga attccgaaaa
    17701 gatcgaaaat gtgacgacct acttaacaaa acccatagat cagtatctga tgtatgtata
    17761 cgaacagcaa aacagtaccg atgaattagt ttctttccca aatcaaatca aagatctcta
    17821 cgcaaattat gaagaactaa gtgcgatata tatcgtgttg aatcaactac cggagtatta
    17881 tgaatcttct agagaaaaca aaggtgggga aataaaaaag gggacaccaa aattactaga
    17941 cgccttttat atcaagttgc ctatcacaca aggaggagct gagtcaggaa gtatttttat
    18001 tggttttcaa aagaaagaac tagatatgat tttagaaaat ctaacgtcat tcaatggact
    18061 ctctttatac atgatttctg gaacgacaaa tcgactatac acattccatg ataataaaat
    18121 agctaaagaa acgtttgaaa gacaagaaat gatcatcaca gagagtctga agaaaaattc
    18181 acaattgccg attgacgatc tggaaaaaaa caattttgtt caacaccaag aactgtctga
    18241 tgattatcgt atattagctg tactagataa aaaaagtgta aaaatcgaaa cgggaaaaaa
    18301 tcttgccccg ctgatcattg gtatcattat tttagacaat atactgctta tctttcttta
    18361 taaaacattc aaacgatatt cacagcaagt atctatcgtc atgcaggcca tggatcaaac
    18421 agcagcaggg aatttagaga cgcggatcga cataacaaac acggaatatg aactgaagga
    18481 actttcaatc ggtatcaacg aaatgttgga tagcatcaat cagtttgtag aagacatcta
    18541 caaattagaa atcaaacagc aagacgcaca tatgcgggcg cttcaagcac agatcaatcc
    18601 acacttttta tataatacat tagaatatat ccgaatgtat gcaatcagcg aaggaagtga
    18661 agaattagca gatgttgtgt atgcgttttc cgctttgctt cgaaacaata cgaatcaaga
    18721 gaaaacgatc actttaaaag aagaactgga cttttgcgaa aaatatgtct atttgtatca
    18781 aatgcgttat cctaatcgag tagcctatca ttttatgatc gatcctgatt tggaaaagat
    18841 cgaagtacct aaatttgtga tccagccatt agttgaaaat tattttaaac acggtattga
    18901 ttttactcga ttcgataatg cgctaagtgt caaagtcttg caggaaggca aacgggtaag
    18961 gatcatcatc aaagataatg gaaaagggat gactgaaaaa cggctaaaac agattgaaga
    19021 aaaactgtct catcctaaag tggaacttca cggttctatc ggtctgcaaa acgtgaatga
    19081 acgtttacgt gccagcttcg gcagcagtta ttatatgtcg cttgagaata atgagacagg
    19141 tggactgacc gtttctatta cattcaagga gggatgagcg tgtatcaagt gttattggta
    19201 gatgatgaat acatgatttt gaatgggtta aaaaagataa ttgattggca aagtttaggc
    19261 tttcaaatag tagcaacagc agagaatgcc aaagaaggac tagctgtact tgaacagcga
    19321 cagattgatt tagtagtaac ggatgtgaca atgccagaaa taaatggact ggaatttatt
    19381 gaagcggctc aaaaagaacg tcataatttt gaatttatga ttttatcagg ataccaagaa
    19441 ttcgattatt tgaaaggagg tatgcaatta ggcgccgtga attatttgat gaaacccgtg
    19501 aataagttcg aattggttga aagcctcaag aaaatcaaaa cacggttaga tcagcaaaat
    19561 gaacaaaaaa atcaacaaga aatctatcaa gaactgttat tcagtcaatg gttgaatgaa
    19621 gaattggatg aaataagtga agaagaaata ataggacaaa taagtgagaa gcagcatagg
    19681 atagtattga ttcaactggc aagaactcat gatactctta tcaatagatg gttgcaagaa
    19741 cggcaacaaa tcttttacta tcaacgaaat tacggagacc gtgtattgat cacgttgttg
    19801 ttggaggcag atgaagttga tggcttctgt tgttttgttc aagaaaactt tcctgatcag
    19861 gaatggctga tcagtatcgg ggaagagaca gattctttgg acaaaatccc tgaaacattc
    19921 caacaagcga aagacaatct acagttgcat caattttatg gagataaaga cgagcatgtt
    19981 ttttacgcag tacaagcaga aacaaaagat caaaccatcg atttttcaag tttcagacgc
    20041 gtgctgcaaa ataaacaatt ggaagtcgct caaaaaatga tcactgattt ttttgaacag
    20101 tttcagttag cagccatgat gccggaagat atacgatatt ccagtttttt actgtttatg
    20161 gagattcaac gggaattgat tgatctggaa gatgaggaat atttacaagg aatcgaaaaa
    20221 atccatcagg caaaaacggt gcaagaactt cagcagctac ttctgtcatt catcaaaaag
    20281 catcagagac agaaaaagta cagtgacaat gtggaaaaag taatcgtaat cctgcaccag
    20341 cattatcaag aaccacttac actcaaagaa gtgtcagaaa acctgcattt aaatgtcatg
    20401 tatctaggac agctgttcaa aaaagaaacg aaaaaaagtt tttcagctta tttgaaccat
    20461 ttacgtatgg aaaaagcgaa acaactgctt ttgcacagta atcaaaacat caatgagata
    20521 gcaagtgaga ttggctacaa caacaccacg tatttctcta aattattcaa gaaaatcgtc
    20581 ggcagatccc ctaaagagta ccgagaaaac agtggagaat cataaaagaa aaagccaatg
    20641 taatgaaata acttataaaa aattgtctag aaatctaatt ttgggctgtt cttattactt
    20701 gaggcaaggc agctttttca tgacctctcg ataaacgg
//
LOCUS       DACEDM010000060        20459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEDM010000060 DACEDM010000000
VERSION     DACEDM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.060"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="IWO50_002028"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="IWO50_002028"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0359135.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="IWO50_002029"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="IWO50_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0359136.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="IWO50_002030"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="IWO50_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0359137.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="IWO50_002031"
     CDS             2855..3730
                     /locus_tag="IWO50_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0359138.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IWO50_002032"
     CDS             complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IWO50_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0359139.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4627..5061
                     /locus_tag="IWO50_002033"
     CDS             4627..5061
                     /locus_tag="IWO50_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0359140.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5227..7272
                     /gene="parE"
                     /locus_tag="IWO50_002034"
     CDS             5227..7272
                     /gene="parE"
                     /locus_tag="IWO50_002034"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0359141.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7284..9734
                     /gene="parC"
                     /locus_tag="IWO50_002035"
     CDS             7284..9734
                     /gene="parC"
                     /locus_tag="IWO50_002035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0359142.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10011..12242
                     /gene="pflB"
                     /locus_tag="IWO50_002036"
     CDS             10011..12242
                     /gene="pflB"
                     /locus_tag="IWO50_002036"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0359143.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12486..13247
                     /gene="pflA"
                     /locus_tag="IWO50_002037"
     CDS             12486..13247
                     /gene="pflA"
                     /locus_tag="IWO50_002037"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0359144.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13332..14258
                     /locus_tag="IWO50_002038"
     CDS             13332..14258
                     /locus_tag="IWO50_002038"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0359145.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14440..15333
                     /locus_tag="IWO50_002039"
     CDS             14440..15333
                     /locus_tag="IWO50_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0359146.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15467..15592
                     /locus_tag="IWO50_002040"
     CDS             15467..15592
                     /locus_tag="IWO50_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0359147.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15726..16358)
                     /locus_tag="IWO50_002041"
     CDS             complement(15726..16358)
                     /locus_tag="IWO50_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359148.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16506..17048)
                     /locus_tag="IWO50_002042"
     CDS             complement(16506..17048)
                     /locus_tag="IWO50_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0359149.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17183..17572
                     /locus_tag="IWO50_002043"
     CDS             17183..17572
                     /locus_tag="IWO50_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359150.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17576..18340
                     /locus_tag="IWO50_002044"
     CDS             17576..18340
                     /locus_tag="IWO50_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0359151.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18469..19416
                     /locus_tag="IWO50_002045"
     CDS             18469..19416
                     /locus_tag="IWO50_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0359152.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19546..20286
                     /locus_tag="IWO50_002046"
     CDS             19546..20286
                     /locus_tag="IWO50_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359153.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7183 a         3477 c         4311 g         5488 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     3841 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     3901 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     3961 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4021 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4081 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4141 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4201 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4261 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4321 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4381 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4441 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     4501 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     4561 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     4621 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     4681 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     4741 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     4801 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     4861 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     4921 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     4981 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     5041 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5101 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5161 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5221 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5281 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5341 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5401 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     5461 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     5521 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     5581 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     5641 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     5701 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     5761 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     5821 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     5881 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     5941 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     6001 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     6061 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     6121 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6181 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6241 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6301 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6361 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6421 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     6481 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     6541 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     6601 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     6661 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     6721 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     6781 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     6841 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     6901 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     6961 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7021 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7081 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7141 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7201 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7261 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7321 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7381 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7441 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     7501 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     7561 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     7621 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     7681 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     7741 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     7801 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     7861 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     7921 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     7981 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8041 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8101 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8161 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8221 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8281 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8341 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8401 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     8461 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     8521 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     8581 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     8641 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     8701 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     8761 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     8821 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     8881 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     8941 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9001 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9061 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9121 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9181 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9241 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9301 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9361 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9421 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
     9481 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
     9541 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
     9601 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
     9661 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
     9721 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
     9781 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
     9841 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
     9901 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
     9961 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10021 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10081 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10141 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10201 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10261 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10321 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    10381 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10441 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    10501 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    10561 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    10621 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    10681 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    10741 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    10801 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    10861 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    10921 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    10981 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11041 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11101 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11161 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11221 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    11281 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11341 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11401 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    11461 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    11521 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    11581 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    11641 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    11701 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    11761 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    11821 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    11881 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    11941 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12001 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12061 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12121 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12181 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12241 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12301 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12361 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12421 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    12481 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    12541 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    12601 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    12661 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    12721 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    12781 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    12841 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    12901 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    12961 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13021 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13081 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13141 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13201 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13261 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13321 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13381 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13441 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    13501 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    13561 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    13621 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    13681 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    13741 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    13801 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    13861 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    13921 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    13981 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14041 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14101 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14161 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14221 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    14281 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14341 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14401 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    14461 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    14521 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    14581 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    14641 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    14701 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    14761 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    14821 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    14881 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    14941 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    15001 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    15061 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    15121 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    15181 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    15241 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    15301 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    15361 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    15421 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    15481 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    15541 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    15601 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    15661 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    15721 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    15781 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    15841 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    15901 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    15961 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    16021 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    16081 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    16141 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    16201 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    16261 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    16321 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaattgat
    16381 aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt aatcaaaata
    16441 ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt ttttaagttt
    16501 gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc tgaaatatat
    16561 tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag gcgatacagt
    16621 tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc ttccagcccc
    16681 attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg caaactttta
    16741 tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa tgccaatttg
    16801 tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata gacaaataaa
    16861 ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata aatcgtgatc
    16921 tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa catgaaacgt
    16981 aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa catttttgga
    17041 tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa aatcgtaagt
    17101 gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata atagaattat
    17161 aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg ccagccacgt
    17221 gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc caattgaggt
    17281 agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg tttttttctt
    17341 tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga atttttctat
    17401 ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt gcttatttcc
    17461 cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc agacaactgt
    17521 tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt aacaattgaa
    17581 tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg ttctgaaaga
    17641 attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga caagtaagga
    17701 gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga tcctgccaga
    17761 tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct atacattttg
    17821 ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt tagaaaaaag
    17881 aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa tgacaacatg
    17941 cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg gttcccctgc
    18001 acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt atgtcgtacc
    18061 aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat tgcagccgga
    18121 aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa aaatccctta
    18181 tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa aagaaattgt
    18241 ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg aagaagcttc
    18301 cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga aaatcactca
    18361 tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt
    18421 tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta
    18481 gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat
    18541 ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat
    18601 taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta
    18661 tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc
    18721 gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac
    18781 gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc
    18841 gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta
    18901 gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat
    18961 caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact
    19021 ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat
    19081 cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact
    19141 tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct
    19201 ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa
    19261 atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt
    19321 ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt
    19381 tttcctgaac tagttatcag agagacaacg aattaagtct tattactata tatatattta
    19441 tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct
    19501 gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga
    19561 cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac
    19621 aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg
    19681 tttgaacaga tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag
    19741 cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa
    19801 ggaaaattac cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa
    19861 aaactttttg gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag
    19921 catactacac gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct
    19981 aaatatgtac ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat
    20041 ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg
    20101 ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg
    20161 ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa
    20221 cgctcattac aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt
    20281 aaataagtga tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt
    20341 ccttatttta tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag
    20401 cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca aactatttt
//
LOCUS       DACEDM010000061        20401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEDM010000061 DACEDM010000000
VERSION     DACEDM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.061"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(71..790)
                     /locus_tag="IWO50_002047"
     CDS             complement(71..790)
                     /locus_tag="IWO50_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0359154.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(921..2072)
                     /locus_tag="IWO50_002048"
     CDS             complement(921..2072)
                     /locus_tag="IWO50_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0359155.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2289..3029)
                     /locus_tag="IWO50_002049"
     CDS             complement(2289..3029)
                     /locus_tag="IWO50_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0359156.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3033..3356)
                     /gene="rsfS"
                     /locus_tag="IWO50_002050"
     CDS             complement(3033..3356)
                     /gene="rsfS"
                     /locus_tag="IWO50_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0359157.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3413..4006)
                     /locus_tag="IWO50_002051"
     CDS             complement(3413..4006)
                     /locus_tag="IWO50_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0359158.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3990..4637)
                     /locus_tag="IWO50_002052"
     CDS             complement(3990..4637)
                     /locus_tag="IWO50_002052"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0359159.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4650..4961)
                     /gene="yhbY"
                     /locus_tag="IWO50_002053"
     CDS             complement(4650..4961)
                     /gene="yhbY"
                     /locus_tag="IWO50_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0359160.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4977..6086)
                     /gene="yqeH"
                     /locus_tag="IWO50_002054"
     CDS             complement(4977..6086)
                     /gene="yqeH"
                     /locus_tag="IWO50_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0359161.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6083..6610)
                     /locus_tag="IWO50_002055"
     CDS             complement(6083..6610)
                     /locus_tag="IWO50_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0359162.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6790..7575)
                     /locus_tag="IWO50_002056"
     CDS             complement(6790..7575)
                     /locus_tag="IWO50_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0359163.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7568..8437)
                     /locus_tag="IWO50_002057"
     CDS             complement(7568..8437)
                     /locus_tag="IWO50_002057"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0359164.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8438..9817)
                     /locus_tag="IWO50_002058"
     CDS             complement(8438..9817)
                     /locus_tag="IWO50_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0359165.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9825..10244)
                     /gene="fabZ"
                     /locus_tag="IWO50_002059"
     CDS             complement(9825..10244)
                     /gene="fabZ"
                     /locus_tag="IWO50_002059"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0359166.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10269..10745)
                     /gene="accB"
                     /locus_tag="IWO50_002060"
     CDS             complement(10269..10745)
                     /gene="accB"
                     /locus_tag="IWO50_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0359167.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10748..11983)
                     /gene="fabF"
                     /locus_tag="IWO50_002061"
     CDS             complement(10748..11983)
                     /gene="fabF"
                     /locus_tag="IWO50_002061"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0359168.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12002..12739)
                     /gene="fabG"
                     /locus_tag="IWO50_002062"
     CDS             complement(12002..12739)
                     /gene="fabG"
                     /locus_tag="IWO50_002062"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0359169.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12751..13668)
                     /gene="fabD"
                     /locus_tag="IWO50_002063"
     CDS             complement(12751..13668)
                     /gene="fabD"
                     /locus_tag="IWO50_002063"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0359170.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13775..13999)
                     /locus_tag="IWO50_002064"
     CDS             complement(13775..13999)
                     /locus_tag="IWO50_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0359171.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14052..15014)
                     /locus_tag="IWO50_002065"
     CDS             complement(14052..15014)
                     /locus_tag="IWO50_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0359172.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15024..15464)
                     /locus_tag="IWO50_002066"
     CDS             complement(15024..15464)
                     /locus_tag="IWO50_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0359173.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15770..16735
                     /locus_tag="IWO50_002067"
     CDS             15770..16735
                     /locus_tag="IWO50_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0359174.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(16792..17022)
                     /locus_tag="IWO50_002068"
     CDS             complement(16792..17022)
                     /locus_tag="IWO50_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0359175.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17108..17995)
                     /locus_tag="IWO50_002069"
     CDS             complement(17108..17995)
                     /locus_tag="IWO50_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0359176.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18084..19307)
                     /locus_tag="IWO50_002070"
     CDS             complement(18084..19307)
                     /locus_tag="IWO50_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0359177.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(19324..20370)
                     /locus_tag="IWO50_002071"
     CDS             complement(19324..20370)
                     /locus_tag="IWO50_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0359178.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         5495 a         4524 c         3472 g         6910 t
ORIGIN      
        1 aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa
       61 aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca tcttctagat
      121 cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat agtgttacct
      181 cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct ttttctttta
      241 atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt tgtccttctt
      301 cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg atctcgtcag
      361 cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa accgctccgg
      421 aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct gctgttcgat
      481 tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca aatccttcat
      541 agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt gctcggtcaa
      601 taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt agtttttggt
      661 ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc ccaaattttg
      721 cataaatccg gctattatta gcatctttag cagttttctt tgctacgata tttgcccatt
      781 tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca aaaacgtcct
      841 gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt ccttcattct
      901 tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt ctgttccgga
      961 atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc ttcttgtgcc
     1021 tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa gaatcttctt
     1081 ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac aggaatctct
     1141 tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt ccatgtatat
     1201 ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc agctgctgtt
     1261 ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc cagcaaagta
     1321 gaagataaca accgataacg caaatacggc caataagatt cccaagtctg cagcgaacaa
     1381 gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt tccttcttct
     1441 aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat tttttcatga
     1501 tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata cttggcattt
     1561 tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag cgtaatatcg
     1621 ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt ttgatcagac
     1681 agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg tccgaaactg
     1741 ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt
     1801 tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc ccattcgacg
     1861 ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg atgccattta
     1921 tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc aatgacgacc
     1981 tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt gtgaaaagga
     2041 ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag ttatcgataa
     2101 aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg aactatataa
     2161 actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga gcaatgccta
     2221 tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa ctcgagcagg
     2281 caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc tgatgctgct
     2341 tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc cagcattctt
     2401 aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg aataaatcga
     2461 ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg
     2521 cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg atagctatat
     2581 tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa tcggccattt
     2641 tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct atttttcata
     2701 tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc tgataaatcg
     2761 agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg ctggctggca
     2821 atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc ttttgcaaag
     2881 gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt gtcttcttgt
     2941 aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat cacttcatcg
     3001 taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact aagatcgact
     3061 agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg cgcatggaaa
     3121 acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc ttttccttca
     3181 atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc tgtaattgcg
     3241 ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa caaggaaaca
     3301 tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc ttcaatcgca
     3361 atttttaatt gttctttact atctatgatg atttcctcct aatatgagca gcttattttt
     3421 ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt tcttgcgcaa
     3481 gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga tcagcccatg
     3541 caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata tagtctgcta
     3601 cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc acccggattg
     3661 catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa gcgccaacaa
     3721 gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg tctcttacga
     3781 ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa
     3841 tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct gtttcttcta
     3901 ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt tgcatcaggt
     3961 tttctctaga ataagcagta tattcattac tatagtccat attcatacaa cccttcattt
     4021 tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc ttctttgatc
     4081 ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca aattacagga
     4141 tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac catagaagtc
     4201 agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc gataataaag
     4261 taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata ggtgtaactt
     4321 ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc ttccaccgct
     4381 aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc aacatgaggt
     4441 ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc agcttgttca
     4501 gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat ccctacttgc
     4561 tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg aacaaatgtc
     4621 ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt tgatagcctt
     4681 tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat gatttggacg
     4741 atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat ctcatctgta
     4801 ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc aatttgaacg
     4861 atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag atgatgcgct
     4921 ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct tcttccttaa
     4981 atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc aattactact
     5041 ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca
     5101 gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg aggaggctgt
     5161 aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt cgttcgatga
     5221 atatttacat catttgacac ataagcaata aatgaagcac gattgccgct gataaaatca
     5281 aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata aactttaggt
     5341 ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta gtgcgccatt
     5401 tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc
     5461 tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc ttgtactccc
     5521 gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt tactccgacg
     5581 atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc cgtcatctca
     5641 tgagatttct tagcactagt taataataca tctactggac gaattcctgc ttcgtaagca
     5701 cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa tatatccact
     5761 ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc tgggattagt
     5821 gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt ttgccctaat
     5881 tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt ctcattataa
     5941 tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc tttttccaaa
     6001 gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac ggcaccacag
     6061 ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata tcagggtgtt
     6121 ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt gtcttccaac
     6181 tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga ttggctccac
     6241 gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct aaatccaatt
     6301 gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca acatacaaaa
     6361 gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa acaacaacaa
     6421 cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct gttccatctg
     6481 gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg atgcctagat
     6541 tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt ggtttgtaat
     6601 ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag tactcctcaa
     6661 ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc gtctttcaga
     6721 aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa atttagaaac
     6781 aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt tatgctgatc
     6841 taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt gtgcgacttt
     6901 atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa cgattcctaa
     6961 ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg cgcgactacc
     7021 gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa tcgtttcttc
     7081 cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc caccttcacc
     7141 aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat ttctggcaat
     7201 agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg ctgtattgat
     7261 caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta aagcttttcg
     7321 atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca gatcccgtcc
     7381 tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc caccaacaat
     7441 cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat cgaagatctc
     7501 ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa cgacatcatg
     7561 tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga agcgtatgga
     7621 ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga gataataaga
     7681 attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc
     7741 ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct tccattgcaa
     7801 aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa aaaagacctt
     7861 cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga gaaaagattc
     7921 cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga ctttcctttg
     7981 ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata cttcccatga
     8041 taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt gctttaccag
     8101 tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt tccgtatatt
     8161 cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca aaactttttt
     8221 catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat ccgcaatgag
     8281 ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa tttggacact
     8341 ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct ctattaggat
     8401 tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct atctccgttt
     8461 ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta
     8521 tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg attccttctg
     8581 tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc gtatttccat
     8641 ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat cccgggtaaa
     8701 ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt tggatcttcc
     8761 ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat tcgattgcgt
     8821 gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct gctatctcca
     8881 cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt tccacttgga
     8941 ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga aactcgatcg
     9001 ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct cctagtgctt
     9061 ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt tgattgttgc
     9121 gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga tcacctaaga
     9181 tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata tcatcatttc
     9241 caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg
     9301 attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc atcactggat
     9361 aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca ccagtacttc
     9421 ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc ttatttccca
     9481 ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct tcacacatgg
     9541 aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct tcggcttttg
     9601 tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg gcttttgccg
     9661 ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca tctgcttctg
     9721 aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata cgtacagcga
     9781 tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc aatcataaag
     9841 gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt gcctattccg
     9901 gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc cggtgtaact
     9961 ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc cttgaattgt
    10021 tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat caatacccct
    10081 ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat cgttacattt
    10141 ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat caaaagcatt
    10201 ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt ccctctttcc
    10261 tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac aactgactcg
    10321 ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc attcatcagt
    10381 ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc tacttttttg
    10441 aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg cgaaacaacc
    10501 tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc agttgttgct
    10561 tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt ttgaaccata
    10621 ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc ttcacgcaaa
    10681 tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat ttcattgata
    10741 tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg ccaaacccta
    10801 atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg acaactgtca
    10861 agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat tgatgttcta
    10921 atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa tgtccagtca
    10981 tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg atttggattg
    11041 cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg atatatccga
    11101 cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta gatgcaccag
    11161 aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg tatccaacga
    11221 tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca agaactagaa
    11281 cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga atcgaaccac
    11341 gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca atccccatct
    11401 cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca tgtttgatgt
    11461 tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg atagctgtac
    11521 aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca
    11581 ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga ataacttggt
    11641 tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac cggtcttgat
    11701 tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg tagattccat
    11761 agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta tcgtaaggga
    11821 aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg
    11881 gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa
    11941 taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg ctctcctttt
    12001 cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg atatatggat
    12061 tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg
    12121 gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc atatcagttg
    12181 tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct tctcgtgcca
    12241 cagattttgt taatcctact acgccagctt tactagctgc ataatttgct tgaccaatat
    12301 ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc tgcttcatca
    12361 tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata tccagacatt
    12421 ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt ccggcattgt
    12481 ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt tgtcccgctt
    12541 tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat gcttcgattt
    12601 cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt gctccagctt
    12661 ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg ataaatacat
    12721 tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt catcaaatgt
    12781 ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca taaatccgct
    12841 taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt ttaacttatg
    12901 gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc ccgcgattgt
    12961 ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa
    13021 ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg
    13081 aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt ttaatagtaa
    13141 aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag gtgtattata
    13201 atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct cgatcgtttc
    13261 aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag cttctgtcat
    13321 ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg cattactagc
    13381 tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga tgattccatg
    13441 ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct gtgtatattg
    13501 cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt agccgagaat
    13561 ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat cttttcccat
    13621 accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt ttttctccaa
    13681 tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat tcctatttca
    13741 ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt tctacgtaag
    13801 tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg tcaaattcat
    13861 cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag tcttcttgga
    13921 tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg atgatttctt
    13981 gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt ttatttttat
    14041 aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc gccatagcct
    14101 gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac tgcttcatcc
    14161 aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt ggtcaggaac
    14221 ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag gtttgcctga
    14281 tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt gtttgcaacg
    14341 tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata cgaaccttct
    14401 gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa tgagctactt
    14461 ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt cgcttctaag
    14521 agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga ccaatcgatt
    14581 agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca gcggatcatt
    14641 ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat atcaaaagcg
    14701 atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa
    14761 tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag atttttctgt
    14821 tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt tgttacacat
    14881 cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc catcatcttg
    14941 gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc agtaatcatt
    15001 ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg caagtttttc
    15061 agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat gttcttgacc
    15121 atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt cgttagtccc
    15181 aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc tttttttact
    15241 aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt ggctacttct
    15301 gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc tgtgattgac
    15361 agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat atcattgaag
    15421 acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca ccgtccacta
    15481 attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt ttcaaaatgc
    15541 aaccttaaaa gatcattttt ttactatatg agcttttttt aagattttac tcatttaaaa
    15601 caactgataa atcagcattt ataatttctt ttattagaaa aatcatctat ctcacatatt
    15661 ttgattatca aagtattttc tgtgaacaga actatagaat tccctaaaac ttgtccatta
    15721 tttcaaaatt tgttatagtc tttttattca aagcgtaaaa ggagatccta tgttaaaaga
    15781 aaaaatgaaa actctcccca tgctgccggg tgtctatctt atgaaagacc gagatggtaa
    15841 ggtcatctat gttggtaaag caaaacggct caaaaataga gtaagcagct attttcatca
    15901 gaataaacag cattcaaaaa aagtattacg tatgattcat catataacag attttgattt
    15961 cgtcgtagta gacacggaat tagatgcttt attgctcgag tgtcaattga tccaacatta
    16021 ccgtcctttt tataaccgac aaatgaatta tttcagtaat tataattatg tacatattac
    16081 caataaggga tttgtattaa cagatactcc tacggctcga acatacgggc cttttcgtct
    16141 ctataaaaaa atgccgtcta ttttgcgtat aatggaagaa acttatcaaa tgccttggtt
    16201 gtctgaaatt agtttgttag ctttgcgagt ccagcttcct gacctacagg aaatgacttt
    16261 tgaacagaaa aaaaaggaac tccaagggct gtttcaaggc cgaaataaaa agctactgac
    16321 ttacttgaaa aaaagacaac aacatttcat ctatcagttg aatttcgaaa aagccggtat
    16381 gcttcaaaaa gatatcgaat tagttactta ttttattaga cggatccagg aacaaaagca
    16441 gtttttaaga acaccatcgc tcaccttcag catgccatta gcagcagatg agagtcagaa
    16501 aaagcactac ctcgtttgtt atggtcaact tgcagaaaca atgattacct ctggtgacgt
    16561 ttctcctgac ttctattatg aaaagaaaga agctcattta tctttgaaac gtcaattgtc
    16621 aaaagaagaa atcgatcctg tacaaatctt gatcagttac cgcaaaaaat tagaaaaaga
    16681 acagtttgaa attgaactgt taaacaaaaa agaagctgaa aagcaactaa attagttgct
    16741 tctcggcttc ttttaggcat cttcaacttc aaatgtcaat tatcctaaaa tttattggat
    16801 taagtcatag atttccatcg ctacgatatc gatattatca aattcgtatt gctgatgagt
    16861 atctgtttca gttaattcaa atgtatcggt cgctttatca taagttactg tacacttctc
    16921 tacgccttct ttttcaaaac ggcgaacttg cagttcatta tctgctgact cgaccatagc
    16981 ctccagtcgt tttatgattg cgactaattg tgaatgtgtc atcggaggtc ctccttttct
    17041 tatttctgtt atattgtatc aaaagcatag cctacttgca ttgattttct ctaaaaaaac
    17101 tcgtttttta tgattttttt tctggtattt ttccttcagg ccaccaaagt ttgatcaaag
    17161 cttgggttcc gtcattctca aaacctttat ctgccaactc ttcatatagt ttcaatgctt
    17221 gggtagttgc tggcaataca agatccattt tttctgcttc ttctaatgca atctttaaat
    17281 ctttgataaa atgtttgaca aaaaaaccag gtgtgtaatc ttcttttaaa atacgaggag
    17341 aataattgct taaagaccag ttagctgcac ttccacctga aagcgtctca atcactttct
    17401 ttaaatcaag tccgtttttt tgtgcataga caagcatttc cgtcattcct gtcatcgtac
    17461 ctgcaatcat gatctgatta gccatttttg tatgctgacc tttcccagca gaaccatgca
    17521 atgtaaatgt cgtaccaaat tctttgaata aaggcagtac ttgatcataa acggcttctt
    17581 ctccaccgac catgatcgtc agtgtaccat tctttgctcc taagtcacca ccggataccg
    17641 gtgcatctaa cgcatctgcg cctttttctt ttgctgtttt tgcaattttt tctgccaggc
    17701 ttggtgtatt tgtagtcaaa tcaactaaaa tatgtcctga gatatctgca gaaaaaatcc
    17761 ccgactgtcc atagtagatt tcttctacat ctttggggta acctaccatt gtaaagagaa
    17821 tattactttg ttctgctacc gcttttggtg tatctgccca gatagctcct tctgttacta
    17881 aaggatcagc ttttgctttt gtccgattat agacagctac ttcgtgtcct gcttctagta
    17941 gatggcgtgc gacagcactt cccatgacgc ctgttccgat aaaaccaagt ttcatttttt
    18001 cgacctccta aaaatcaaaa atccttgctt ctatgctagt atacgctaga aacaaggatt
    18061 ttttcataaa aaatgtttga attttatctt ttaaatgaat ttaattttat tttgattcgt
    18121 tcaatcgagt cttcttcttc ttttcttcgc atctcttctt catgataaac aaccatgtct
    18181 tgataaaatt cgatcatagc ttcgccgaaa tgtgtagatg aaatatcata aagtttagct
    18241 tctaaaactt ttggatcttt aggaacttca ctggaaatat aatcaataag tgctggtgca
    18301 aagtcttcgt cggtttcaaa agttttccct aatgaaggat gatcgaacaa tcggttcaaa
    18361 taagcatttc cttccacgac acaaggtgtt ccggctgcca tcgcttctgt ataagtaagc
    18421 ccttgtgttt ctgaagtaga agcactgaca aaataatctg ccgctttata ataaagtgct
    18481 acatcattgt tgtccacttc tcctgtgaac tgcacaaatt cttctaactg cagttctgag
    18541 atcaattcct ctaactcttc tacataaggt ccttttccta cgataactaa tctggcgtta
    18601 ggataagcgg agatgacctg aggaaaccca ttaatcaagg cttggatatt tttctcataa
    18661 gaaatcctgc ttaaagaaag aatcatcaaa tggttttcct gaagacctag tgactccctt
    18721 aatttgctga tatcctgctg agtgatatca gaacgtttga atttttcaat gtcgatacca
    18781 gtaggaatca cgcgaagcgg tgctattaca ccatagctgc gtaatgtatc aataacacgt
    18841 tcacttggac aaactacacc tgtcgtatga tttgcaaata atctagacaa gtattttaca
    18901 tgggattgcc tgatcacttt tcctttaccg atataatgaa ggtagtcttc atacatcgta
    18961 tggtaagtat ggatcagagg tattttcaac ttcttagcta ccatcttccc aagtatccct
    19021 gctccgaatt ctgtatgggt atgaataaga tctagttcta gttctttggc aaccaaataa
    19081 gcataccaca tgcctctaac aacaactctt cgatccttga aagaaataaa tggtacactt
    19141 ggcattcgaa tcacatcttt ttccaactta tccgcattag gatcggtcgt tgtaaaaata
    19201 tagacttcat gcccatgtct ttctaattct tgttttaatg ttttgatcga tgtagcaact
    19261 ccgctgactt gcggaaagta ggtatcggta aaaaagccga ttttcatgtt tttcgcctcc
    19321 aaactagtcc ttgacctgat ttactttttt taatttgcta gagacttttt cattcagtac
    19381 actttcgtat acatttttaa gttcttcacc gattttattg atacttcgct cttttgctgt
    19441 ttgataaccg gcttgtgtcg tactcggtag ttcttgattg gtaatctgtt ggatcagtcg
    19501 gataaattct tcgtttgtct tccccatata gcagttcttg ctatcttcta accagccatt
    19561 atacactgga atatctcgaa caagtacgtt ttgtttgctt gctaaagctt ctaaaacaac
    19621 gatcccttct gtctcttcat aagaagggaa aaagaataga tctgcacctg aatacgcacc
    19681 ttctatgatc tctccacgaa tgtaaccagg aaacaggaca ttttcaggat gatcttcttt
    19741 tacaattttc cggatgttcc gaggaatcga gtacatggga acatgaccaa accaaataaa
    19801 tgtgtattcg ggcatcttct tagcaatttc tacgaaatca atgattcctt tacgttcgaa
    19861 aaacaaaccg acacaaatga ttactttttg atccggagac agtttgaaat attctctgaa
    19921 cttttgttct ttcattggat cagggtgata tttctctaag tcgatcccat tggaaatagc
    19981 ttgaatcggc acacggatac catagctttc caacaactgt tttgaataag gtgttggcgt
    20041 gatcagatag tccgcttttt ggtacaatcc taccagatat ttttttacga taggagaaag
    20101 ctggttcgat ccgataaagg aatttcggaa atcttcctct gtagaatgcg catgatagac
    20161 aacttttttt ccttctcttc tggctttatt gaccatatta tgactattga ttccgtatgt
    20221 attgatgtgc aggatgtcat aatcttcttt ttcatctagt gtataagaga taccgacttc
    20281 cgtcagagca cgtttttgat gatcaagcgc acgcccgatt ccagattttg caagcatctt
    20341 ttcactttca aaatataata ataccttcac cttacgtccc ccttcactta tatagtataa
    20401 c
//
LOCUS       DACEDM010000062        20246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEDM010000062 DACEDM010000000
VERSION     DACEDM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.062"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            292..1431
                     /locus_tag="IWO50_002072"
     CDS             292..1431
                     /locus_tag="IWO50_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0359179.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IWO50_002073"
     CDS             complement(1498..2073)
                     /locus_tag="IWO50_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0359180.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IWO50_002074"
     CDS             complement(2078..3052)
                     /locus_tag="IWO50_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0359181.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IWO50_002075"
     CDS             3335..4303
                     /locus_tag="IWO50_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0359182.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IWO50_002076"
     CDS             4416..4694
                     /locus_tag="IWO50_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0359183.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IWO50_002077"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IWO50_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0359184.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IWO50_002078"
     CDS             5160..5906
                     /locus_tag="IWO50_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0359185.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IWO50_002079"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IWO50_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0359186.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IWO50_002080"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IWO50_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0359187.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IWO50_002081"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IWO50_002081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0359188.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IWO50_002082"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IWO50_002082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0359189.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IWO50_002083"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IWO50_002083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0359190.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IWO50_002084"
     CDS             10643..11185
                     /locus_tag="IWO50_002084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0359191.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IWO50_002085"
     CDS             11208..12764
                     /locus_tag="IWO50_002085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0359192.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IWO50_002086"
     CDS             12778..13680
                     /locus_tag="IWO50_002086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0359193.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IWO50_002087"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IWO50_002087"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0359194.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IWO50_002088"
     CDS             15123..15545
                     /locus_tag="IWO50_002088"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0359195.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IWO50_002089"
     CDS             16014..16244
                     /locus_tag="IWO50_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0359196.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IWO50_002090"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IWO50_002090"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0359197.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IWO50_002091"
     CDS             17655..17831
                     /locus_tag="IWO50_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0359198.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IWO50_002092"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IWO50_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0359199.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="IWO50_002093"
     CDS             18326..18709
                     /locus_tag="IWO50_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0359200.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="IWO50_002094"
     CDS             18878..19762
                     /locus_tag="IWO50_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0359201.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IWO50_002095"
     CDS             19879..20112
                     /locus_tag="IWO50_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0359202.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         6737 a         3532 c         4395 g         5582 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgcctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaagta
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg ctcgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttcc
//
LOCUS       DACEDM010000063        20071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEDM010000063 DACEDM010000000
VERSION     DACEDM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.063"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            169..1935
                     /locus_tag="IWO50_002096"
     CDS             169..1935
                     /locus_tag="IWO50_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0359203.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            1963..3771
                     /gene="uidA"
                     /locus_tag="IWO50_002097"
     CDS             1963..3771
                     /gene="uidA"
                     /locus_tag="IWO50_002097"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0359204.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            3943..4344
                     /locus_tag="IWO50_002098"
     CDS             3943..4344
                     /locus_tag="IWO50_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0359205.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            4338..4583
                     /locus_tag="IWO50_002099"
     CDS             4338..4583
                     /locus_tag="IWO50_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0359206.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(4907..5128)
                     /locus_tag="IWO50_002100"
     CDS             complement(4907..5128)
                     /locus_tag="IWO50_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359207.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(5290..5362)
                     /locus_tag="IWO50_002101"
     tRNA            complement(5290..5362)
                     /locus_tag="IWO50_002101"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5327..5329),aa:Lys,seq:ctt)
     gene            5438..5668
                     /locus_tag="IWO50_002102"
     CDS             5438..5668
                     /locus_tag="IWO50_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0359208.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            5712..6881
                     /locus_tag="IWO50_002103"
     CDS             5712..6881
                     /locus_tag="IWO50_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0359209.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            7455..8024
                     /locus_tag="IWO50_002104"
     CDS             7455..8024
                     /locus_tag="IWO50_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0359210.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            8011..8871
                     /locus_tag="IWO50_002105"
     CDS             8011..8871
                     /locus_tag="IWO50_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0359211.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            9061..9765
                     /locus_tag="IWO50_002106"
     CDS             9061..9765
                     /locus_tag="IWO50_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0359212.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            9770..11605
                     /gene="walK"
                     /locus_tag="IWO50_002107"
     CDS             9770..11605
                     /gene="walK"
                     /locus_tag="IWO50_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0359213.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            11602..12918
                     /locus_tag="IWO50_002108"
     CDS             11602..12918
                     /locus_tag="IWO50_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359214.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            12919..13788
                     /locus_tag="IWO50_002109"
     CDS             12919..13788
                     /locus_tag="IWO50_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359215.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            13843..14652
                     /locus_tag="IWO50_002110"
     CDS             13843..14652
                     /locus_tag="IWO50_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0359216.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(14755..15384)
                     /locus_tag="IWO50_002111"
     CDS             complement(14755..15384)
                     /locus_tag="IWO50_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ0359217.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            15554..16846
                     /locus_tag="IWO50_002112"
     CDS             15554..16846
                     /locus_tag="IWO50_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0359218.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(16922..17584)
                     /locus_tag="IWO50_002113"
     CDS             complement(16922..17584)
                     /locus_tag="IWO50_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0359219.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            17809..18093
                     /locus_tag="IWO50_002114"
     CDS             17809..18093
                     /locus_tag="IWO50_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0359220.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            18144..19769
                     /gene="groL"
                     /locus_tag="IWO50_002115"
     CDS             18144..19769
                     /gene="groL"
                     /locus_tag="IWO50_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0359221.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6981 a         3138 c         4104 g         5848 t
ORIGIN      
        1 aataaagttt tcttttatcc tattttggtt aataaagaaa aagaatcatg ggaaaactta
       61 agaactgata ttttatttaa atgtttatcg ggagagtata tagatattga acaaaaatat
      121 atagatactt tttggaaaaa tcttactaat aagtagagga gaaagaaaat gactataaaa
      181 gtagacttac gaaaaaaacc atatcattta gatgaagatg ctatttcttg gattgaagag
      241 acgattgatg ctatgacttt agaagaaaaa ataggtcaat tatttgttaa tatgggatct
      301 agtcgtgata aaaattactt aagtagtatg gtcaatcagt accatattgg agctgtacga
      361 tataatccag gaactgctga tgaggtatat gatcaaaact atatcctaca aacagaaagc
      421 aaaattcctt tattaattgc tgctaataca gaagcaggtg gtaacggagc ttgtacagat
      481 ggtaccgaag ttggtgtaga agtaaaaatt ggtgcaacaa atgatgcgaa atatgcttat
      541 gaattaggga gagtttctgg aattgaagca tcggctattg gatgtaactg gagttttgct
      601 cctattgtag atattaatag aaattggaga aatcctatca tttctagtcg atcttttgga
      661 caaagccctg ataaagtttt agaacttagt ttagcttata tgaaaggtat tcaagagagt
      721 ggaattttgc ctgcagctaa acatttccct ggtgacggta ttgacgaacg agatcaacat
      781 ctatctttta gtattaatag tttaagtaca gaagaatggg ataattcttt tggaaaagta
      841 tactcaggct taatagaagc tggattacct tcaattatgg caggtcatat ccatatgcca
      901 gcttattcaa aacattttaa tcttgatctg aaagatgaag atttacttcc tgcaacttta
      961 tcaaaagaat taatcactga cttacttaga ggaaaactcg ggtttaatgg attagtagtt
     1021 actgatgcta gtcatatgtt aggaatgact ggggcaatga aacggtcaga attattgccc
     1081 acttctattg cggctggatg tgatctattc ctatttttta atgatccaga tgaagacttt
     1141 aattatatga tggaaggtta ttacaaagga attatttcag aagagcgttt acgtgatgca
     1201 ttaagaagaa ttttaggctt aaaggctaaa ctaggtttgc ataaaaaaga aaagaataaa
     1261 attttgttac cgaaacaaga agctatggaa aaaattggtt taattgaaaa taaatcaatt
     1321 tttaaagagg tagctgatca agcaattaca ctagttaaag ataaacaaga tatttggcct
     1381 attgtaccag aaaaatataa aaggatttta ctggtagatg tacatggggt aaaaggtggg
     1441 tttggtgaag tagttgctgg aaatagacct caggccattg atttattgaa acagattttg
     1501 tcagcaaaag gatttgaagt tgaaaaatac atttcacctg aagaagaatt gaaaggatta
     1561 tcgcaagaag aaaaaattaa aaatatagct ggaatatatg ctgctaagag accaatcaaa
     1621 gatttaactg aaaaatatga cttgattatt aatatagcta atgttaaccc aggtacagtt
     1681 caaagaattg tttggccagc aagtaaaggt actccggata ttccatttta tattcatgaa
     1741 attccaacaa tttttgtatc tgtacagtgt ccgtttcatt tagctgatgt gcctcaagta
     1801 aaaacttata tcaatactta tgatggaaaa caagaaactt tagaaatatt agtggacaaa
     1861 ctaatgggcc tttctgaatt taagggaata agtccaatag atgcttattg tgggttaaag
     1921 gatacattta tttaatattt aaattatata ggagtgattt acatgttata tcccattatg
     1981 acagagacga ggcaagtaat tgatttaagc ggtctttgga gatttaagtt agatacaaat
     2041 gatgacttac aagaagaact tgctttagaa ccaatggttg aaaaagagag ttatccaatt
     2101 tcagttcctt cttcttataa tgatttattt gaaacagaga acattagaaa tcatgttgga
     2161 tgggtgtggt atgaaaaaga agtgattctt cctaaaaaat tattagaaga aagattagtt
     2221 cttcgttttg gatctgcaac acatgaagca aaagtatttt tgaatggaaa attgattaat
     2281 caacacaaag gagggtttac gccatttgaa tcagaaatta atcagtttat aaaagatgga
     2341 agtaacagaa ttacagtggc tgtaaacaat gttattgacg atagcacttt acctgtaggg
     2401 attttgaaag aaacgataca ggaagatgga gacataaaaa aagaaaattt ggttaatttt
     2461 gactttttca attatgcagg cattaataga cccgttaaat tgtatacaac tccgaaagac
     2521 tatataaagg atgtcactat taatacaaaa ataattgatt cagatgcaaa tgtagaattt
     2581 aatatcgata cagaatggac cggagaaatt ctaatagcaa tctatgacga agaagctcaa
     2641 cttatagcag aagctaaggg gaaagaagga gtgattcaac ttaagaaccc aacactttgg
     2701 gagcctctaa atgcttactt atatagtatg aaagttagtt taattaacga aaataaagtg
     2761 attgatacgt acacagaaga atttggcatt cgtagtattc aggtttcgaa tgggaagttt
     2821 ttgattaata ataaaccttt ttactttaag ggatttggga agcatgagga cacgtatatt
     2881 aatggtcgtg gtttgaatga agcagtaaat atcaaagatt ttaatttaat gaaatggatc
     2941 ggtgctaatt catttaggac ttcacattat ccttattcag aagaaattat gcgcttagca
     3001 gatcgagaag gtattgtagt tattgatgaa gtacctgcag taggattaca tttgaatttc
     3061 atggctactt tattagatga caacgtagaa aaacatgata cttggaaaga aatacggaca
     3121 gaaaatgctc acaaaatggc gattaaggaa ttgatagaac gcgacaagaa ccatccgagt
     3181 gtcgtaatgt ggtctattgc aaatgaacca gattcggatt ctgaaggcgc taaagaatat
     3241 tttgaacctt tagttgaatt gactaaagaa cttgaccctc agaagagacc tgtaacaatc
     3301 gttacttact taaagtcaac accagatatt tgtaaagtag gagatattgt cgatgtttta
     3361 tgtttaaatc gctattatgg ttggtatgtt tcaggtggag acctaaaaca tgcaaaatat
     3421 ctgttgaaaa aagagctaga tggatggatg gaaagatgtc ccgatacgcc aataatgttt
     3481 acagaatatg gagcagatac agtagctgga cttcatgata cagtaccaac aatgtttaca
     3541 gaagagtatc aagtgggtta ttacgaagca aatcacgaag taatagataa atgtgacaat
     3601 tttgttggtg aacaagtgtg gaattttgca gattttgaaa caagtcaagg gattttaaga
     3661 gtgcaaggaa ataagaaagg aatctttact agagatagaa agcctaaaat gattgctcac
     3721 tatctaaaga aacgatggtc atcaatacca aatttcagtt ataaaaaata atttaccttt
     3781 tataaggaat ataaaaaagg agtggctgct taattgccaa ctcctttttt ttataaaaag
     3841 attttctgtt taaagaagga tatttttttc tagttttttc gaaaaaaagg cgctttctga
     3901 ttgttgtatt agtggaaagg ggaaaaaagg agcatattta tcatgcaaca aaattacaat
     3961 gagaaatcta tacaacatca atttgatgcc tactgtaaaa aagtattacg aaacgaaact
     4021 aagtctattc gaaaacgaaa cattaacata acagataatg aggaaccatt gacgaattca
     4081 aatgaaggta agtactccca aactcatttt gatgaacaag atgtctattt cttatttgga
     4141 atggaaatat taatttcaga tcaaaagtta tcggcagcca ttgatcattt atcggaaata
     4201 agaagaaagg ttatcctact ttattatttt gcaggtttta atgatactga aatcggtaaa
     4261 atcctcaata tgagtacaag tggtatctgg tatcaacgaa agaaggcagt tgaacaatta
     4321 aaaatggggt atgacttatg gtagacaacc ccaaaaaacg gcagtatcca ttagttcctc
     4381 tatccattat ccaatctgct ctagaagaag atccaatttc aatcaagatg atactcaaac
     4441 attatcatag ttatatagct aagttatgtt tgacaaatgg atttaatgag gcggggcaat
     4501 ttattaccta tgtagatgag tatatgctaa gacagctaga aattaaattg atagaagcga
     4561 tcttaaaatt taaaattaac taactaaaaa gtcccctttc cacttattct gtttaaaaga
     4621 atcttctaat agttaaagga ttagtagatt cttttaaaca gaaaacctat tgatcattga
     4681 aatttaaata cattctatca gtacgttaac acttcaagga gcatatttta gttgcgccat
     4741 gatttcttac tgtaaagaag gagcgataaa cattctaaaa taatttttat agtcaataaa
     4801 aagcgatgat ttgaagttgg gataatgata cttccgtaag gctcgccgta aacttggaga
     4861 gaggtgaaat tcctatgtgc ttttgacaaa agcagctgat taagaattat ttccatatgt
     4921 tgatttcaac attttttcta ttatttttaa gaccaaattc agtgaggcaa atagcatcat
     4981 gtaaatttag tttttctatt gaacgttctc cactttttag tttttttata gtactcaaat
     5041 ttaatcctgt atgtttagag atctcatttg cagtatggtt ttgtaaagtt tctcggatta
     5101 ttgttatatt agcaatttca ttacgcataa ttatgttcct ttcttatcaa aaatatcaat
     5161 gactacagtg ggcttttagt gaaaaaaagg ggtactacag tctcttttga aaacaaaaaa
     5221 atacagtata tacgttttct aaatgctaag caccaacgtt tacactgtat ttaagggttt
     5281 ggcacccaat gggctgtgag gggctcgaac ccgcgacccg atgattaaga gtcatctgct
     5341 ctaccaactg agctaacagc ccaaattaat actttgttat tctaacacct aaaataaaaa
     5401 agtgcaatac ttattatcag aattgagagt gaattttatg gattttcaag attatgaaaa
     5461 aatatttgaa agttatccat cgatattgaa aattactgat ttaactaaaa tgttaaatat
     5521 tggaaaaaat aaagcttata atttgctgaa agatagatca atagaaaata taaaaattgg
     5581 agcaaggtac aaaataccta aaaaagctgt cattgagtat atattgaaaa tacaagatga
     5641 aggtaaaaat catgctataa taaaatagtc aaaatttttg ccgatatttc agtttaagag
     5701 gagagattga attgaaatta agagcaaaca taattgaaaa aagaggaaca ttacaagtag
     5761 ttgtatctta taaagactct aacggaaaaa ttagacaaaa gtggaaagat acaaaattaa
     5821 aaaagaaagg aaataaaaaa cgagcagaaa aaattagaga tgaattttta ctagaattgg
     5881 aaagtcaatt agctatggaa tgtgaatgtg ctaatccaaa tttattgttt tatgactatt
     5941 tgcttaatta tttggaagta tcaaaaaaac aagtagcatt taacacatat gttgggtata
     6001 agcattacgt ccataatcga atttacaagt ttttttatcc taaaaaaatt aaactaaata
     6061 ggttaaagcc ttatcattta caagagtttt atcaatatat gttaaatgat aactgtacag
     6121 caaatacggt tattcattat cacgcattta tacgcaaagc tcttcaagag gctgttatta
     6181 cagaattgat aagtacaaat gtagcagata aagtaagaaa gccaaaaaag aaacaatttg
     6241 tttctcaagt ttataatcaa caagagatct tgaaattact tgacattttg tcagaagaaa
     6301 aactctattt ggtagttctt ttaacagcat tttatggatt gcgaagaagt gaagttttag
     6361 gattaaaatg gtctgctatt gattttttaa acaaaaaaat catgataaac catgtaatca
     6421 tagaaaatcc agagaatatg tcacagttaa taaagaaaga tcaaacaaag aactcttcca
     6481 gctatcgtac attacctttg gttgaaacga ttgaacgagc attagtaaat caagctaaat
     6541 ggcaacaaga taatcgtatt cttttaggag atgattatat tttagaagat aatgagtacg
     6601 tatttactat ggaagatgga cggttgatga agcctcaata tgtaacgcat cgtttgagta
     6661 agttgattaa gaaaaatgga ttgaaaaaaa ttcgctttca tgacttgcgt cattctaatg
     6721 cttcaataat gttagatagt ggccagaata tgaaatccat tcaagaatgg ctgggacatg
     6781 catcgtattc gacaactgct aatttataca cgcatttaac cgcaggagta aaagatcgtg
     6841 cagcagaagc attagaaaac gcatttgggt ttaaagaata aaaaaacaaa aaacaatatc
     6901 tttggggtat cgcttcttgt ttttagaatt tttttgttgg caatttgttg gcagtatgtt
     6961 ggcaatgaaa aaattcacaa actaaaaacc tatttataaa gctttataaa acttttttca
     7021 caaatagagc atttggttaa gagtcagctg ctctaccaac tgagctaacg gcccgtacaa
     7081 aaagtactta ataatagtag cactaagaat tttaaaatgc aagactacat ttaaagaaaa
     7141 tcagaaaaat ctttacttcc ttataggaaa tcattcatag attattcaca tttgctcgct
     7201 attatataag cataccaaca aacaaagttc ttgcaagaga acaaacccta acactttttt
     7261 cattttttac tcctttcccg ccgtctcccc ggcgggtctc ctgttcttaa cgataaaaga
     7321 caagaaggct tagccttctt gttttttgtt ttcatggaat cccgctaatc aaatcaccta
     7381 gttaaagcaa ctagattgaa tgtttatcat aagtctgata tcatgtttat gaaatgaaaa
     7441 aaggagcggt tatgatggaa aaaatgcgtc agcaattaat ggagtggggg aaggaactag
     7501 aaacattccg tctgcctcac tgggaagaac tccctgactt agaactttat atggaccaag
     7561 tcagaacgtt agttgatcgt tatctttcac ctgtgataca aggagaaaag catccattgc
     7621 ttacctcttc tatggtaaac aattacgtta aactaggttt gataccagca ccagttaaaa
     7681 aaagatataa caaagaacac gtagcatttt tacttgcaat cactacgttg aaacaagtat
     7741 tgactattcc tgaaatcaaa gaagggatat tgttccaagg aaaaacagta ggaattcgtg
     7801 aagcctataa tctattttgc gatgaacaag aagcagctgt gtggatggtc agccaattag
     7861 cacaaggtaa atctcatccg caaaaattcg accaggcaac ccctgtagaa tatattgctg
     7921 taagagcggc aactttatcg tttgcaatga aacttcttgc ggaaaaaacg attgttctag
     7981 aaaccgaata tttaaaagag gagaaaacaa atgaaaaaca ataaaatcgc aattttagta
     8041 gattcaggta cggatgtgcc agaagaattg ataaaaaaat acgagatgta tatgatccca
     8101 ttgaaaatta tttataaaga tcaggtatat acagataaaa ttgacattac ctctgaagag
     8161 atctatcaac gtttgccaga ggaaatccct ggtacatctt tgccggatgg tgagtcgatc
     8221 acagaaatct ttgagcaaat caaaaaagac ggttatgaaa aggtacttgc ggtaacaatt
     8281 tccagtggat tgagcgggac atataatgtg gttcgtttaa ttgcacaaca gcaagaagaa
     8341 ttggaagcat atgtactaga tacgaaaaat attggtattg gtgcaggttt ttctgcgatc
     8401 caagcagcaa aatggctgga agaaggagta gaatgggatc aactgatcag taatctgaat
     8461 gagttagtcg aaagaacaaa agttttcttt aatgttgcga cattggaata tcttcaaaaa
     8521 ggtggacgga tcggtttagt tgcttctatt gtaggaaccg ctttaaagct taacccaatc
     8581 atctcatgta atgaagaagg gatttattac acagtaggaa aagctagggg acgtaaaaaa
     8641 agtttggata aaacggttgc ttacgtcaaa gaaaaaatcg gtaatgcaaa ggtcttcaac
     8701 ttagctgttg cccatgggga tgcgaaagaa gaagcaattg aaatggaagc gagacttaaa
     8761 agagagttcc cacaagcaaa ccaaatctac tttggccaga tttctccggc attagtagtc
     8821 cacactggtc cgggattatt gggtgtcggt gtacaagttt tagaaggata aatcaaaaaa
     8881 aggaaacact gaggtgaaaa tcagtgtttt ccttttttat tgaagttaga acaaaaaata
     8941 ataaagtatt ttgcactttc attttaattt tctcttttgt tttataggca aagatgaaac
     9001 aaaagcagaa atacggtata atgagaaggt atgacaataa tatataagag gtggctcttt
     9061 atgaaaaaag ttttagttgt cgatgatgag aagccgattt cggatatcgt caaattcaat
     9121 ttagccaaag aaggctatga tgtttatact gcatatgatg gggaagaagc cttggaaaaa
     9181 gttgctgagg tagaacctga tttgatttta cttgatttga tgctacctaa aatggacggc
     9241 ttagaagttg ctcgtgaggt acgcaaaact tatgatatgc cgatcattat ggtaacagcc
     9301 aaagattctg aaattgataa agtcctaggc ttagagttag gcgccgatga ttacgtgacc
     9361 aagccatttt ccaaccgaga attagttgca cgtgtcaaag caaatcttcg tcgcggtgcc
     9421 acggctgcga aggaaccaga agaggcagca cctgcggaat tgacgattgg tgatttgacg
     9481 atccatccag aagcttacat ggtgacgaaa cgaggagaaa caatcgagtt aacacatcgt
     9541 gaattcgaac tactctttta cttggcgaag caccttggac aagtgatgac aagagaacat
     9601 ttattacaaa cggtttgggg ttatgattat tttggagatg tacgaacagt ggatgtaact
     9661 gtacgtcgtt tgcgtgaaaa gatcgaagat aatccaagcc atccaaatta tttagtgaca
     9721 cgtcgtggtg tcggttacta tttgcgtaac ccagaacagg agtaattgga tgaaaagaaa
     9781 agtccgcttt tttcgatcag tcaattttaa gatcgctatt acctttattc tgattttatt
     9841 gatctcgatc gaaattattg gtgcctactt tatacgtggt ttagaacgtt ccacgatcaa
     9901 tacatttatt aaagatatga atcaaacggt ggaatcatta gcaacaacaa tcagcccgga
     9961 attgaatcga aaagataatg cagatgatga agaggtaaat gcgaatatca aaagatttat
    10021 tgaaaacagt gcgactagcg atatcatcga aatacgggtc gttgacgaaa aagggatcat
    10081 ccgaggaaca accgatgtca atgaacaaag cgctgtggga aagaaaaatg attatgttga
    10141 tatcaatgat ttcacgacta aacgttatgt tgctttagat aacgacgata aacgggtgaa
    10201 tatcaatgtt caaccgatcc tgtccccgac cggcgatgcc gttatcgggg ctctttacgt
    10261 caaaagtaat atcgaacaaa aatattcgga aatcaataac acagcagtca ttttctttac
    10321 tgcctcacta attgcggcat ttatttcaat gatcgtatct gttttagttg ctcgttcgat
    10381 tacacagccg atcggtgaaa tgagagaaca agccattcga atcgctagag gagactacag
    10441 tcggaaagtc aaagtgcatg gacaagatga acttggacag ctggctgata ccttcaatca
    10501 gttagctgaa cgtattgaag aagctcaaga tacaatggaa gctgaacgca atcgtttgga
    10561 tagcgtgcta tcccatatga cagacggcgt cattgcgaca gatcgcaggg gtaaagtcat
    10621 tacgatcaat gatatggctg tttccttgct agatatcaaa aacgaggaag caatcggaca
    10681 atcgattttg acacttttag atatcgaaga agaatatacg ttgcggaaat tattagaaga
    10741 tccaaacgaa atggtacttg accgttcaac cagtacattg gaaagtgatc agatgatctt
    10801 gcgagtagac ttttctatga tccgtcgcga atcaggtttt atcagcggat tagtagctgt
    10861 cttgcatgat gtgacagaac aagaaaaaac agaacgggaa cgtcgtgaat tcgtctctaa
    10921 tgtttcccat gaacttcgta caccgttgac aagtatgcgt agttatatcg aagcattgag
    10981 cgaaggtgcg tggaaagacg aggaagtagc accaaatttt ttgaaggtaa cgctagatga
    11041 aacagaccga atgattcgaa tgatcaatga cctcttaaat ctatctcgca tggatacagg
    11101 taatacccag cttcaattag aatatgtcaa tttcaacgag ttagtgaatt ttgttcttga
    11161 ccggtttgat atgatggtgg gaaatcagga aaaaaattat aagatccgtc gtgaatttac
    11221 gcaaagagat ctttgggtcg aagtagatac agataaaatc atccaagtag tcgataatat
    11281 catgaataac gcgatcaagt attcaccaga tgggggaacg attacttgtc gcttattaga
    11341 aacgcataat aacgtggttt taagtatcac cgatcaagga ttggggattc ctaaaaaaga
    11401 tctgaaccga gtgttcgagc ggttctatcg agtagataaa gcacgtgcaa gagcacaagg
    11461 tgggacagga cttggattag ctatttctag agaagtgatt aaggcgcatc gaggcgctat
    11521 ttgggcagag agcaaggaag gaaaagggtc gactttttat atctcgctgc catatgaacc
    11581 ttatgaggag gaatggtggg aatgagaatc tctgaaaaag tcgttcgtat aggactcatt
    11641 ttgatggttg cgttaagcat atacttctct tatgcaatct ggctgagccc agcaggaaaa
    11701 agttcaatca acgtagatga gacaaacact caaatgatcg agtctcaaag caatcgaaaa
    11761 acttccgaag tctttcttcc gcttcatgtt acatggcacc attcgggaat gatccaagaa
    11821 accaacagtg aaaatctcgt gtaccgattg caattgatca tcgataaagc aagatttgga
    11881 aagctttctg aagtagtttc aggagacaaa tcacagtttg aacaggtgaa aaatctgaac
    11941 tctggttttg agttggccta taatgcgcct tttttgctaa gagaatacaa ggaagcattt
    12001 gatttagatt tagatgtgtc gaacttggca aaagaaagcg aaaatatgta ttttactaga
    12061 atacaattcg atcaaggaaa taaaaaaatc cgatttttga attattcgaa ccatctcgta
    12121 tatgaagcaa atatttcgtt gaattggaca gatatcaata aagaattaac agcaaaagac
    12181 acaaaatgga cagaaatgac aacagaacca tcgacgatcg acacgcaata tgctacgaaa
    12241 cattcgatca aactgaaaaa gtatagttat atcctgtcac agcaacccta tacacttttt
    12301 agaaatgctt ttttccagaa accagatagc gtgaaaaata atgatgaaag caatgaattg
    12361 attttttacg gtgggaatga aacaatgtct atccattcgg aaacgcaagt agttgatttt
    12421 cgtggagagc tgccagagaa aaaagaaaat gctagtgtat tttctgaaag tttcccctat
    12481 attagcaaat taggcagttc tttagggaac ctcagatatt ttgaccgcag tgacaatacg
    12541 atcgattatc ggatattcgt ggaaggcttc ccggtttttg ggacagacag cagaggacag
    12601 attggtgtgg aagttggcgg tgaaacgaat ggttctgtgc aagtgaatat ccaaaccagt
    12661 ttgaatacga ttcaagtgcc gataccttca gacgaagaag tagaacttgc aagtacgaat
    12721 gaaatcatcc agcagttaaa agctaaggga gcagatagca acaagattgg ttcagtgatc
    12781 gttggctata catggcaaaa tgtcaaagaa acgaatcgtg tcgttgatct cttacctgaa
    12841 tggtatgtga aatataaaga taactggtat tcagctaacg accttctgga aaaattacct
    12901 ggattggagg cgaattaatt ggatttcaaa aaaatcgaat ggatattttt cgtcgctttt
    12961 ttagggttga atgtgttttt attcagcagt tatcatgacg cggtatacca agaaaacaat
    13021 gtcattcgct ctaatcagac tgaatcaatc caacaacgtc tggctagcga cgatatcact
    13081 tacacgggga agatttcgtc tgagaacaag caaggctatt atcttagcgc ggagcagacc
    13141 aatcttagca cttcattagc gaattatcgc aaggctggta aaagcggctt gctggcaagt
    13201 ggtgtgtttt tagacggtga tgttttgacg aaatctttat ccagttcaga tgctgaaaag
    13261 aacgtgatcg atccagataa aattagtagt tccttggaag attttttatc taacaaagac
    13321 aatattttat tcggtaatga ttatgtgtac acgaaaaact tttctcattt agaagaagaa
    13381 ccactagaaa tccaagcaag ccaagagtat gaaggcatcc cattcaatga tgatacagca
    13441 aaaatcgttc tttctttaga aaaaaacgat aatgattata gcatcacgaa atacatgcag
    13501 acgcatttat ctgatatcga gcagctgcgc gaaaagacgg aactgtatac ggaagaggaa
    13561 gcaatcaata cattgtacat caataacaaa ataccaagag gatcaaaaat tttatggcgt
    13621 cagctagctt attcctgtat tctgaaagtc cgggaaaaaa atgtgtatgt tcctgtttgg
    13681 catgtagcga tcgaaacaag cgataaagtg gtccaaattg agagtgtcaa cgctttcagc
    13741 aatacgatcg tgacaaacaa tactgtacca aaagtggaag atcaataaaa aagcgagtat
    13801 aatgaaaaaa gtttacaaag taagaaggga agtaggagtc aaatggctga agaaaaacca
    13861 gcttttaaaa tcagtatcct agcaagtgga agttcgggta attcacttta tatcgaaagt
    13921 gaaaaaaaac ggcttttggt cgatgcagga cttagcggta aaaaaatcac ttccttgatg
    13981 gctcaaattg atcggacacc ggaccaatta gatggaatac ttgtgacgca tgaacatcga
    14041 gatcatatac atggcgtggg tgtcttggct cgtaagtatc atttggatgt ttttgccaat
    14101 cagcagactt gggaagcgat ggaaccactg attggaaatg taccagtgga acaaaaacat
    14161 ctatttgaga tggggaaagt cagaacattc ggcgatatcg atattgagag tttcggcgtc
    14221 tcccatgacg cagcggctcc gcaattttat cgtttccata aagaaaatcg gtcattcgtc
    14281 atgttgacgg acaccggata ttgcagtgat tatatgcgag gtgtcattga aaatgccgat
    14341 ggttatttga tggaaagtaa tcatgatctt gagatgttac gcatgggacc ttatccgtgg
    14401 aatttaaaac agcgtatttt aggagataga ggacatcttt ccaacgaaga cggtgcactt
    14461 gtcatgactg atgtgatcgg taatcggacc aaacgaatct atcttggcca tcttagcaag
    14521 gaaaacaata tgaaagaact tgctcattta acaatggaaa atgttttgaa agaaaaagat
    14581 tttggcgttg gacatgattt tgaaatctat gatacagatc cagatgaagc aactgagcta
    14641 ttttcgattt aaacagttca atcaataaaa atataaaaag cagaagataa aatcaatcag
    14701 ggttggtttt atcttctatt tttatttttt tagcatatta aaaaaataac cgtattatcg
    14761 actgactaca ctgatgtgct gcttttcatg tttctcatcc agtatctcat ctactaatgc
    14821 aatcgcgtaa tctgccatgc tgatcgtact gtcgccagca gcgtttcttt gtaaataatc
    14881 atcacttaac aaataagtac ctgtacgttt gccatgtgga tcaaataaag aagctggact
    14941 gatataagtc caatgtagat ttttagattg tttcaatttt aacaatcctt cgtacatatt
    15001 gaaagctgtt ggatagaagg gtgcattttt aggtacttgg tcaatcatac gagtcgtctt
    15061 ttctgtatct aagaataacg aagaggcacc cccaacgaca attaagcgtg tatcggttcc
    15121 ttcaagcatc tcaatcaaat gggacaaaga agtttgatgc atttcttctt ttcctttggg
    15181 agcgttgaaa gcatcaacga caacatcaaa agatgcaaga tcagtagttg tcaactcaaa
    15241 taaatccttt tgtataaacg gaacatctga tggcaatttt tctggatgac gcacgattgc
    15301 cgtcacgtct aaatctctgg acaatgcttc gtctaaaata aatgaacctg catggccagt
    15361 agctccaatg agtgcaattt tcataaaaaa cctccttttt tattcctgtc cattgtaagc
    15421 agttgctgaa tattcgtcaa agaatccgat ttttgaaaag ctcagtgcgc attcctttca
    15481 tggttcgtga tcattgggta tagttaaatt aagaaaactg attgataaaa atagaagttg
    15541 aaggagataa gaaatgaaag agtggttaga tcgtgccttt ttaccaatga taacatctgt
    15601 tgtgaatctt agacccttaa ctgctttgcg taatggaatg acttacacga tcccaataat
    15661 tattgttggc tcgctgtttt tcttatttgc tcattttcct gtggattaca tcgctcagtg
    15721 gatagacaaa acaggatttt ctatgtattt tacacaagta taccaggcta catttgatgt
    15781 gatagccttt tggatcgttt ttgccattgc atacgcttat gcaaaagaag aagacatcga
    15841 tcgcttacca gtcggcataa cagccttgtc tgcttatcta ctgcttattc agccactaaa
    15901 tactcagata gatactgtct ggacaggtgc tgaagggata ctaggagcag ttacaactgg
    15961 gctcatcatc ggatggggct atgcacgttt agtaagactt gcgaaagggt ggaagcaaag
    16021 agagaagata cctgaaaatg tttggcagtc ttttacttca ttgattccaa tcattgttat
    16081 agtcactttt acgcttatcg gttcagcggt ttttcaacag ttgttcagaa tgactgttgt
    16141 tcaaggagta tggacatatg tccagcttcc cattcaatcg ttcatagaca cgttgtttgg
    16201 tgtgctttta ttaggttttt ttgttccgtt tttatggtta tttggtcttc atggatctgc
    16261 gatggtcaat gggttggtta gtcccgtctt gcaagcgaat tcattagcga atgcagaaat
    16321 cttagcctca ggacaagagt tgacggtggc aaatggcgga catatcgtta ctcagcaatt
    16381 tctcgatcag ttcatgacgg ttacgggtgc tggattgacg ttaggggctg tattttttat
    16441 gacggttttt gcgaaatcga agaaatacag agaattagga aatcttgctc ttttaccagc
    16501 ctttttcaac atcaatgaat caattatttt ttccacaccg atcgtgatga acccgatgat
    16561 ggctgttcct tttattttcg caccggtcgt ttcaggattg attacctatt ccgctttata
    16621 ttttggattc gttcctttat ttacagcagt ccaagtccct tggacaacac cgccgatcct
    16681 ctcgggtttc ttagtaggcg gagtgcctgc agcgatttta caaggtatcg tgttgtcaat
    16741 tagttttttc atttatttcc catttttgaa aaaaatagat tctgcaaact gcaaaaagga
    16801 aagacagaca aaaaatgatg gagcagcaga aaagatgaac gactaaaaaa caaaaatact
    16861 cctgtcgctt tagattggga aaatcaccaa tctaggatga caggagtatt tatagtcatg
    16921 tttatactag ccctttttgg acagctaatt gaacaagaac aactaatgtg ttcattccta
    16981 cgtgtgtgat catcgatgtc cagatccgac cagttgcttt gtactctagg ctgaagaaga
    17041 aacctaggaa aaagtagatt agcagatgac catcattatg agcaaacgca aacagcaaag
    17101 agctgatcac tgctccgacc caaaaatttg aatatcgact gatgatcccc agtatactgc
    17161 ggcggaatac gaactcttcc atgatgggtc cagcaacagt tgtcgccaaa ataaaagcgg
    17221 gtgcttccat gatcatctgg atgatatttt gtgtattctc ggaaggggtt gctttgccaa
    17281 acaatacaga ttctaacatg acagcgatgc tttgcagaat caatgcgaca aagattccaa
    17341 ccaatccata aataacaatc tttggagcag aagctttttt taccgctgat tcgatcgcta
    17401 agggttcacg ttgcttaaaa taaaaaatag ctaggacaac ggcgcctagt atatacatga
    17461 ctgcagttgc tgtgatcaat tcagacgtag attttacaag tcctatcgaa gctaggatca
    17521 agggtgagat aaaaattaga ccatagcaaa aaatgctgag gaagctatat ttttttaaag
    17581 acatgtaatt cctcctttta agggataaca agcatgcaag gcaagcttat atcgattgat
    17641 ttcaattata tcataatttc aataagcagc tgtcagaata aaatttcttt tctttgattt
    17701 aaaagcttgc aaaaaagagg gagaatgagt attattaaaa atgtaggtta gcactcgaag
    17761 gttaagagtg ctaacagatt aaaaaaagag ttatcggagg gatttatcgt gttaaaacca
    17821 ttaggtgatc gcgtcattat cgaagtcgca aaagaagaag aaaaaactgt cggaggaatc
    17881 gttttagctt ctgctgctaa agagaaaccc caaactggga cagttgttgc agtcggtgaa
    17941 ggccgtttgt tagaaaacgg tgaaaaagtt cctgccgctg taaaagcagg cgaccaagtt
    18001 atgttcgaaa aatacgccgg tacagaagta aaatacgaag gaaaagaata tctgatcgtc
    18061 gcaggaaaag atatcatggc gattgttgag taaatttgga agataaaaaa ataatcaaaa
    18121 ataatcaatg aggtgaatca atcatggcaa aagaattgaa atttgcagaa gacgcacgcg
    18181 cagctatgtt acgcggggta gataaattag cagatacagt gaaagtaaca ttaggaccta
    18241 aaggccgaaa tgtcgtacta gaaaaatctt acggctctcc attgatcaca aatgatggtg
    18301 tgacgatcgc aaaagaaatc gaattagaag atcattttga aaacatgggt gcaaaacttg
    18361 tatcagaagt agcatcaaaa acaaatgata ttgcaggaga cggtacaacg acagcaactg
    18421 ttctgacaca agcaatcgtt cgcgaaggtt taaaaaacgt aactgcaggt gctaatcctc
    18481 taggcattcg tcgcgggatc gaattggcaa caaaagcagc agtcgaagaa ttgcataata
    18541 tctcaactgt tgttgattca aaagaagcca ttgcacaagt agcagctgtt tcttcgggtt
    18601 ctgataaagt tggacatttg attgctgatg caatggaaaa agttggaaac gacggtgtca
    18661 tcacaatcga agaatcaaaa gggatcgaaa cagaactaga tgttgtagaa ggtatgcagt
    18721 ttgatcgtgg ttacttatca caatatatgg taacagataa cgacaaaatg gaagctgttc
    18781 tagaaaaccc atatatcttg atcacagaca aaaaaatctc taacatccaa gatattctgc
    18841 cattgttaga acaaatcttg caacaatcac gtccattgtt gattattgct gacgatgtag
    18901 atggtgaagc attgccaact cttgtattga acaaaatccg tggaacattc aacgtagtag
    18961 ctgtaaaagc tcctggtttc ggcgatcgtc gtaaagcaat gcttgaagat atcgctattt
    19021 tgacaggtgg tacagtaatt actgacgatc taggtttaga attgaaagat gtaacaatcg
    19081 aaaacttagg aaatgcttct aaagttgttg tagataaaga caatacaacg atcgtagaag
    19141 gttctggaga aaaagaagca atcgaagctc gtgtgcaatt gatcaaaaac caaattgctg
    19201 aaacaacttc tgattttgac cgcgaaaaat tacaagaacg cttagctaaa ttagcaggtg
    19261 gtgtagctgt tgttaaagtt ggtgcagcta ctgaaacaga attgaaagaa ttaaaattac
    19321 gcattgaaga tgcattgaat gctacacgtg ccgcagtaga agaaggaatg gtctcaggtg
    19381 gtggtacagc tctagtgaac gttatcagca aagtttctgc tgtagaagca gaaggagacg
    19441 tggcaacagg tatcaagatc gttgttcgtg ctttagaaga accaattcgt caaatcgctg
    19501 aaaacgctgg ttatgaagga tcagtgattg ttgataaatt gaagaacgtg gaactaggca
    19561 ctggtttcaa tgctgctact ggagaatggg taaacatggt tgaagcaggt atcgtagacc
    19621 caactaaagt aacacgttct gccttgcaaa atgccgcttc cgtttctgcc ttgttattaa
    19681 cgacagaagc agtcgttgca gataaaccag aaccagcagc gccagcagct cctgcaatgg
    19741 atccatcaat gatgggcggt atgatgtaag aaaaagcaac ttgcttatct acaactgatc
    19801 attagtgaaa gacagataaa aagaggctgg aacagaagcg tttaactcca agaaataaga
    19861 agaaattcac ggaaattgct tttcaaattt ttgtgaattt cagcttattt ccgaaggagt
    19921 tgcttctgct tccgctgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt
    19981 tgtcccacgc tcttttttgt gtgtaattat aatgaaaata gttattttta atagttataa
    20041 tattttatct attaaaagta aatatccatt c
//
LOCUS       DACEDM010000064        19811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEDM010000064 DACEDM010000000
VERSION     DACEDM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.064"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            251..526
                     /locus_tag="IWO50_002116"
     CDS             251..526
                     /locus_tag="IWO50_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359222.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2611
                     /locus_tag="IWO50_002117"
     CDS             539..2611
                     /locus_tag="IWO50_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0359223.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2776..4662
                     /locus_tag="IWO50_002118"
     CDS             2776..4662
                     /locus_tag="IWO50_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0359224.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4674..5621
                     /locus_tag="IWO50_002119"
     CDS             4674..5621
                     /locus_tag="IWO50_002119"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0359225.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5641..6168
                     /locus_tag="IWO50_002120"
     CDS             5641..6168
                     /locus_tag="IWO50_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0359226.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6231..6884)
                     /locus_tag="IWO50_002121"
     CDS             complement(6231..6884)
                     /locus_tag="IWO50_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0359227.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7016..7858
                     /locus_tag="IWO50_002122"
     CDS             7016..7858
                     /locus_tag="IWO50_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0359228.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8016..8912
                     /locus_tag="IWO50_002123"
     CDS             8016..8912
                     /locus_tag="IWO50_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0359229.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8915..9628
                     /locus_tag="IWO50_002124"
     CDS             8915..9628
                     /locus_tag="IWO50_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0359230.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9648..10166
                     /gene="msrA"
                     /locus_tag="IWO50_002125"
     CDS             9648..10166
                     /gene="msrA"
                     /locus_tag="IWO50_002125"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0359231.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10163..10390
                     /locus_tag="IWO50_002126"
     CDS             10163..10390
                     /locus_tag="IWO50_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0359232.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10542..11093
                     /gene="lepB"
                     /locus_tag="IWO50_002127"
     CDS             10542..11093
                     /gene="lepB"
                     /locus_tag="IWO50_002127"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0359233.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11176..11718)
                     /locus_tag="IWO50_002128"
     CDS             complement(11176..11718)
                     /locus_tag="IWO50_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0359234.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11955..12353)
                     /locus_tag="IWO50_002129"
     CDS             complement(11955..12353)
                     /locus_tag="IWO50_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0359235.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12613..13473
                     /gene="ylqF"
                     /locus_tag="IWO50_002130"
     CDS             12613..13473
                     /gene="ylqF"
                     /locus_tag="IWO50_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0359236.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13466..14233
                     /locus_tag="IWO50_002131"
     CDS             13466..14233
                     /locus_tag="IWO50_002131"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0359237.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14289..15146
                     /gene="dprA"
                     /locus_tag="IWO50_002132"
     CDS             14289..15146
                     /gene="dprA"
                     /locus_tag="IWO50_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0359238.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15268..17346
                     /gene="topA"
                     /locus_tag="IWO50_002133"
     CDS             15268..17346
                     /gene="topA"
                     /locus_tag="IWO50_002133"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0359239.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17315..18691
                     /gene="trmFO"
                     /locus_tag="IWO50_002134"
     CDS             17315..18691
                     /gene="trmFO"
                     /locus_tag="IWO50_002134"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0359240.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18889..19794
                     /gene="xerC"
                     /locus_tag="IWO50_002135"
     CDS             18889..19794
                     /gene="xerC"
                     /locus_tag="IWO50_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0359241.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         6873 a         3414 c         4120 g         5404 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta
     2641 taaaaaacaa aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca
     2701 aaaagaacaa ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca
     2761 agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt
     2821 gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg
     2881 attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc
     2941 agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct
     3001 caagcagctg aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc
     3061 cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga
     3121 gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat
     3181 gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta
     3241 gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg
     3301 ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa
     3361 gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc
     3421 cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa
     3481 ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt
     3541 gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg
     3601 agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat
     3661 ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca
     3721 caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa
     3781 acactgctag aacatttaga tttattgatt caatctcggg aaagactagg tattactggt
     3841 aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat
     3901 agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa
     3961 gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa
     4021 acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga
     4081 tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg
     4141 ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg
     4201 gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc
     4261 actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa
     4321 ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa
     4381 gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa
     4441 aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca
     4501 gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc
     4561 actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa
     4621 atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac
     4681 aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa
     4741 caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt
     4801 ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt
     4861 ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg
     4921 aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt
     4981 ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc
     5041 ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac
     5101 acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa
     5161 tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg
     5221 catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat
     5281 tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc
     5341 taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa
     5401 caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc
     5461 atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt
     5521 tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat
     5581 atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa
     5641 atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt
     5701 ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta
     5761 gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg
     5821 attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg
     5881 gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca
     5941 gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt catccatcat
     6001 gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat
     6061 actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag
     6121 cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga
     6181 tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag
     6241 aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca
     6301 tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg
     6361 cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc
     6421 acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc
     6481 cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat
     6541 acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt
     6601 cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt
     6661 gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca
     6721 tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga
     6781 accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact
     6841 tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta
     6901 ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt
     6961 acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac
     7021 aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt
     7081 aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga
     7141 ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac
     7201 aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag
     7261 tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca
     7321 agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg
     7381 attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa
     7441 aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac
     7501 actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt
     7561 tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg
     7621 gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt
     7681 gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt
     7741 caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc
     7801 ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc
     7861 gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa
     7921 attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat
     7981 aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa cgacatttga
     8041 tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta gctgcttcta
     8101 taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat ccaaataaaa
     8161 aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta ggagatcaaa
     8221 ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt tataatttga
     8281 ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag atcttaaagc
     8341 gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt atcacactga
     8401 ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg ttaacggtca
     8461 attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta ttggaagaat
     8521 ttcgatccct caataaaacc gcacctattt acattttagg catctacaat ccattttatt
     8581 tgaactttcc tgagatcact gacatgcaga caattgttaa caattggaac aaaggaacag
     8641 aaacggtgac aaaaaaaatg ccgaactgct actttatccc aatcaatgat ttgctttatc
     8701 aaggattgaa tcaggaagta ggtgtcacag aagaaggaac agatcaaaca actagcacga
     8761 cagaaaatct aaatggcatc aaaaataatg tgctctatga agaagatcat ttccatccaa
     8821 ataatttagg ttatcaattg atggcaaatg cagtgagaga tcagctgatc gagacacaaa
     8881 accaatggtt agagaaggag gatgatcaat aaacatgaaa acaagatctg acgtatcttc
     8941 aaacaaaaca aagaaaacaa attactggaa attcgccttc cttatccttc ttggggtagt
     9001 acttggttcg accgtatttt taggcagccg tatttttgct aatcgggaac cagaactccc
     9061 agaaatacca gctcttacgg aaagacaagg agatcctgtc ttaacgatca attcaaataa
     9121 agaaaaagtc aatcaaatca tcagcttttt ccttagtgag tatcaaaaag atagtgatat
     9181 tgaatataaa ttttatttgg aaaatgaagc actactcaat ggtactttcg aagtactagg
     9241 ttttcctatc gatttctatt tatattttga tccgtatgtg atggaaaacg gaaatgtcca
     9301 actaaaagct agaagtcttt ctatcggaac attgagtttg ccaatcaaag atgtgatgaa
     9361 tatgatcaag agaaactata aattaccaga ttggatcgaa gtcaatacag atgatctgac
     9421 tatcatgctt aggttggatc aattccgaat gcaaaacggg atgtatatta aagcagataa
     9481 aatcgactta gtaaatgatg acattcggtt tagtctatat ttgccaaaag atacctctga
     9541 tgcttcaaac aactcaaata ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa
     9601 agaaaataaa aaatcatcgg atgagtagta ttagaggggg aatcataatg gaacgagcaa
     9661 tatttgcagg aggctgcttc tggtgtatga tccagccttt cgacacgctt cctggtatcc
     9721 atacagtgat gtcaggatat actggagggc atgtaccaga tccgacttat gaacaagtaa
     9781 aaagccaaac gactggacat acagaagcag tggagattat ctatgatcca acattgattt
     9841 catatgaaca attagtagaa ctttactggc agcaaactga tccaactgac gctttcggac
     9901 aattcgagga tcgcggtgac aattatcgcc cggtgatctt ttatttgaca gaagaacagc
     9961 ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc gaacagccaa
    10021 tcgttacaaa aatcgaacca gcccaaccat tttatctagc agaagaagac catcaagagt
    10081 attataaaaa gcatcctgaa aattttaaaa gaaaccatgc acgccgggct gcctttatcg
    10141 aagaaaattg gaggaacgaa gggtgaatcg aagctttttt cattatttaa tgacactgcg
    10201 aggcggtggc acacatgatg ctttatctgt tttcgctaca gaagccggta aagacatcca
    10261 atttcctaag cacagctcta gctacgaaga aatttctaat tatctggaac tgaatgtcga
    10321 ctatttgccg tccatggata tctttgacga agcatgggaa aaatatttag aaaataatca
    10381 tgacatataa gagatagttt tgtactttat gatcaaaaaa gttttttgtc caatagccta
    10441 attgaaagaa ttatttttat ttcagctatc tagtcgcatt tttttgatat ttttcttata
    10501 ataaagaaag aatgaaaaaa agagggatgg attgatagag attgacaaaa aaacaacgct
    10561 atattaatcg cttctggctt gtattcaaat acttacttgt ttctgtattc cttgcgttca
    10621 tgttgcgggg atttttattc atcccggtac cagtggaagg caattcaatg gaaaacgtgt
    10681 tgaagcaagg tgatatggtt gtgatggaaa aattttcgga gatcagacga tttgatattg
    10741 ttgtttttca attagcagat ggcacgattt atatcaaacg agtaatcggt cttccaggag
    10801 aaaatgtcag ctaccaaaat gatcaattga agatcaatgg gaaagtagtt aaggaacctt
    10861 atctaactaa aaacctgaaa tcagatcatg caaatgcatc ctatacgacg gactttacac
    10921 tacaagagct aacgggacaa tccaagcttc cggaagataa ttattttgta cttggagata
    10981 atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat caataaaact gatattctag
    11041 gtaaagcaag atttgtctat tatccacttg atgaaataaa atggatccag tgagaaacaa
    11101 taaagcgtgc agtctgttga gactgcacgc tttattgttt aatccaaaat cagtttacta
    11161 ccttagtacg taaaatcaaa cagctcgttt aaacactttt ctagcctcta aagttttgat
    11221 aaatgaataa acaaccatta tttgaatagg gatttggatc atattcctga tgaatctacc
    11281 tagaaaaatt actttgaaat ctgcaccaaa caaaacagac atcagccaag aattgataaa
    11341 aaggctgact gggatcataa tcaataattg ggcagcgatg acggaaggca ggtttatctt
    11401 tttatgatat aaaaataagc cattaataaa tccaccaagg aaagccgtga taagaaaagc
    11461 agcttgaaac gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca acatttcaag
    11521 caaaccagcc cagatcggtc catatagata acccataagc gccatactta aaaatccgaa
    11581 tgtgatttga aaataagcag gaccaatcgc tatcttgtat aagatgacat caagggctaa
    11641 taaaagagca gaggtcgaca acaatcttgc attcatttcg ggcaatctaa aaacgtcttt
    11701 tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt aataataaaa ggataccata
    11761 aaatcaccaa tgtgtcatga cacattggtg atttacatac actggcatct aacaattaaa
    11821 aaaagggtga gaagactgct tttataaact gtaaaaaaga gattgtacga ataaaaaaaa
    11881 tagaggcgga gaatacttat cgtatcatcc gcctttgttt ttttgttata aaaaacgtca
    11941 gattagttat ctgcttacat cttcatccat acgggttcta atgtatttcc gtctagatct
    12001 tggacttcta atgaaaacat ttcttcttca ggcattccca tatctacttc gtggaaagta
    12061 ccaccatttt cttttgcaat ttgagcaaat ttttttactt catccgcgtt ttcaacacta
    12121 aacgaaatca aagcaccact tgttttttta ttatcagcta tttctttatc ttttataaat
    12181 tttttataaa aatcatgaga gagaagcatt atccaaaaag tgtcatccca taccattgaa
    12241 ccagcttctt cagtagaaaa ttcttcattt tttttaaaac ccagcttttc ataaaaagca
    12301 acggaacgat tgacatcagc aacaggaaaa ttaacaaaaa ccatactatt catttgtttc
    12361 gtctcctttc tgttttgttc gtctatcatc tcttatttag gaaacgctgt caaaaattta
    12421 gattcatata tagaaaccaa aataaaaatt tgcatcatca aaaaagtgaa gatgtgatag
    12481 aatgaggact ggttcacttt cacttaacta atagatggaa tttctttata ataaaaaagt
    12541 gaatttatat tgaaaaagaa aaaaacaagt aaagccgggg aatattccgg tatgaaagga
    12601 atggagagaa caatgaccat ccaatggttt ccgggacata tggcaaaagc ccgacgagaa
    12661 gtttctgaaa aaatgaaata tgtggacatc gtatttgaat tggtagacgc acgattacct
    12721 ttatcttctc gtaatccaat gctagatcag atcattcagc aaaaacctcg ccttgtttta
    12781 ttgaataaag cggatctggc agatccacaa cagacaaagc tgtggcaaga atattttcgt
    12841 aaacaaggcc atcttgcatt agctatcacg gcacaagaaa gtaaaggaat taaaggtcta
    12901 gtacctgcgg ctaaagaagc attgaaagaa aaacgggcaa gagatgctgc tcgggggatc
    12961 aagcctcgcg ctattcgggc aatggtctta ggtattccaa atgttgggaa atctacgttg
    13021 atgaatcgac tagtaggcaa gaaaattgca cagacaggaa ataagcctgg cgtaacaaaa
    13081 ggtcagcagt ggcttcgttt aggtaatgag ttggaattgt tagacacacc agggatttta
    13141 tggcccaaat ttgaagatga agaaatcggc aaaaaactag cgctgacagg cgcgatcaaa
    13201 gaccagcttg tccatttaga tgatttagcg atttacggat tagacttttt tgcccgatat
    13261 tattcaggaa gaatcagcga tcgttatggt ttatcaatcg agcaagaaga aaaattggca
    13321 ccggaattac tgatggatat tacagcaaaa cgtgggtata aagaagacta tgatcgtggc
    13381 agcgaattag tcgtgcatga tattcgtcaa ggaaaacttg gacgatacac actggatcgt
    13441 tgcgaagaaa tggagcaaaa aagtgatgac tgaatccatc caaagtatca aagaacgatt
    13501 gaaaagcata cagagaatag atgatcctac actagaatca ttaagaaatg atccacgcaa
    13561 aggcgtgcag caagcactgg cttcttttga acgacgattg aaaaaggaag cattaattaa
    13621 ggaaaaatat gaagaaatgt tttgctttga aaagcaaggg tacgcacaag gtcaccaatg
    13681 gattgccggc attgatgaag taggaagagg accattagct ggacctgtcg tggcagctgc
    13741 agttatcttg ccgaaaaatg agatcatatt agggctgaat gactccaaac aattatcaga
    13801 gaaaaagcga gaacttcttt atcaacaagt tcaagaaaaa gccctggcaa tcgggatcgg
    13861 gatcgttgat cagcagacaa tcgatgatgt gaatatttat gaagcaagta aattagcgat
    13921 gatccaagca gtagaaaaat tgtccattgt accaagctat ttgttgatcg atgcgatgac
    13981 cttggatttg cctattcagc aggaaaaaat aatcaaaggg gatgcacgat ctgtgtcgat
    14041 tgctgctgca agtatcattg ctaaagtata tcgagaccaa ttgatgaaag aatatgacgt
    14101 gctttatcct ggatatggtt tctcgcataa tgcaggttat ggaacaaagg aacacttaga
    14161 aggacttgaa cgacaaggaa ttacgccaat ccatcgtaaa agttttgcac ctgtaaaaaa
    14221 atatttacaa taaaaaacag ccgcttttac gtatcttatt agaaagagag acgaaaggcg
    14281 gttttatgat gtatcaaata gaagaaaatt tattgaaact tgcttataca aaaggaatca
    14341 gtagtcaagg aaaatgggca cttgctaact ggatgatgca gtatccttat aaagaaatcg
    14401 gatttgaaga catcattaag ataggtaaaa taaccaaaca tcgggagctg tttcgcaaaa
    14461 gttggcagga aattcacgct aactggggca aaatccaatc gaaacagtcg tttatcactt
    14521 gctttgatcc caaatatccc cctcagctgc ttcacttgac ctatccgcca attgtactgt
    14581 tttataatgg tgatctatct ttactttctt gtaacatgtt aagcgttgtt ggtggaagac
    14641 acacttctgc tttagccaaa aaaacagttt attatttact aaaaccagtg atagaagctg
    14701 gatatgtgat cgtcagtggc tgcgcgaaag ggatagatac atacggtcac caagcagcaa
    14761 tctctcatgg tggacggaca atcgcagtca ttggaacagg gatagagcaa gtctatcctg
    14821 ctgaaaatcg tgacttgcaa gaggtgattg caaatgatca tcttcttttg agtgaatatc
    14881 ctcctcaatc cggcccgaaa aagcattgtt ttccaatgag aaatcggatt atcgcagctt
    14941 tgtctaaagg aacatgtgtc tttgaagcca agaaaaaaag cggctctctg ataacagcac
    15001 agcaagcatt ggatcttgga agaacagttt tttctgttcc aggaaatatt ttaacagaac
    15061 attctaccgg atgtcatcat ttgatccaag acggtgcagt ttgcacggtt tcgggtgaag
    15121 atatcttggc agaatttaaa gaataacacc atacttttca tttgcttttc ttgacaaacg
    15181 ttttctgaat ggatatgatt agctacgatt tgtatgaatc ctcttttaat agtaggaaca
    15241 cgacaatgga aaggagtcca ttcagtaatg gcgtataaat atttagtgat tgtagaatca
    15301 cctgcaaaag ccaagacaat tgagaaatat cttggacgaa actataaagt aatggccagt
    15361 gttggccata taagagattt accgaaaagt aaaatgggta tcgattttga aaacaattat
    15421 gaaccccatt atatttctat acgcggaaaa ggcgatgtca tcaaaagcct gaaagccgca
    15481 gcaaaaaaag ctcaaaaagt ttacttggca agtgacccgg atagagaagg agaagcgatt
    15541 gcttggcatt tagcgtacct acttgggttg gatctgaaag aaaaaaatcg ggtggtcttc
    15601 aatgaaatca caaaagacgc agtcaaagca gcttttaagg aaccaagaac gatcgatgta
    15661 gatttagtag atgcacagca agctcgtcgt accttagaca gaatcgttgg ttattcgatc
    15721 agtcctattc tctggcgtaa ggtcaagaaa gggttaagtg caggacgtgt ccaatctgtc
    15781 gctttaaaaa ttattattga ccgtgaaaaa gagatccgag aatttgttcc agaagaatat
    15841 tggagcatcg acggtaattt taaaaaagct cgcaagaaat tcaaagcaaa tttctgggga
    15901 atcgacggta agaaaaagaa attaccagat gcacaaagtg taaaagaagt cactgctaga
    15961 attgatggaa cagacttcga agttaaaaaa gtagaaaaga aagagcgcaa aagaaaccct
    16021 gcgaatcctt tcactacaag tagcctgcaa caagaagctg cccgtaagtt aaactttcga
    16081 acaagaaaaa ccatgatggt agcacagcaa ttatatgaag gaatcacttt gggtaaacaa
    16141 ggaacagtcg gcttgattac atacatgcgt actgattcta cccggattgc cgatacagca
    16201 aaagcagagg ccgcagcatt tattgaagat acgtatggaa aagaattttc tagacatgat
    16261 caccgaaaga tcaaaaatgc tcaaggcgca caagatgccc atgaagctgt gcgtccaacg
    16321 agtgtgcttc gtacaccaga tgaactcaaa caatatttag ataaagatca attgaaactt
    16381 tatacattga tctggtcacg ttttgtggct agtcagatga caccagctat tttagataca
    16441 atgaaagtga cattagaaca aaatggtgtc atcttcattg cgaacggttc aaaaatcaaa
    16501 ttcaaaggat tcatgcaagt gtatgtggaa ggtagagatg acggcaaaga agagaaagaa
    16561 aatattcttc cagaacttga agaaggcgat gtagtccaat ctgttgatat tgaaccaaaa
    16621 caacatttta ctcaaccacc agcacgtttt agtgaggcta cgttgattcg tgcactagaa
    16681 gaaaacgggg tagggcgacc ttccacttat gcaccaacac ttgatacgat ccaaagacgc
    16741 tactatgtaa aattgacaca aaaaagattt gagccgactg aactaggaga aattgtcaat
    16801 tcattaatct gtgaattctt ccctcaaatc gtcgatatcc atttcacagc agaaatggaa
    16861 ggagatttgg ataaaattga agaaggcacg gaagcgtggg tcaaagtggt tgatcgtttc
    16921 tataaaccgt ttgaaaaaga actgacgaat gcggaagaaa aaatcgaaaa aattcaaatc
    16981 aaagatgaac cagcaggatt cgattgtgat gtctgtggac atccgatggt gataaaatta
    17041 gggaaatatg ggaagtttta tgcttgcagt aattttcctg attgtcggaa tacgaaacca
    17101 atcgtaaaag aaatcggtgt gacttgccca gtatgtcatg aaggacaagt cattgaacga
    17161 aaatctaaga aaaacagact attctatggt tgttcccgtt tcccagaatg tgaattcact
    17221 tcttgggata aaccagtggg cagaaattgt ccaaaatgtg acggatatct ggtcgagaag
    17281 aaagtcaaag gcggtaaaca agtagtctgt gtaaatggag attacgaaga agacatccaa
    17341 aaataataga aaagaggttc tttcattgaa aatggtaaat gttgttggag cagggcttgc
    17401 cggaagtgaa gctgcttggc agatcgcaca agcaggagtc cctgtccgtt tatatgaaat
    17461 gcgtccagtc aaatcgactg aggcgcatca cacaagtaat tttgctgaac tagtatgttc
    17521 caattcatta agaggaaaca gtttggctaa tgcagtaggg ttattgaaag aagaaatgcg
    17581 ccgattgaat tcggtagtaa tcacttcggc agatgaaaca gcagtgccag ctggtggtgc
    17641 cttagctgtt gaccgcgatt ctttttctga aacaattacc cgaaaagtca aagagcatcc
    17701 attagtaaca gtgataaacg aagaaattac tgaaataccg gaaggaatca ccgtagtagc
    17761 aacaggacct ttgacttctc atccattagc agaaagcatc aaagcattca atggttcaga
    17821 tggtttttat ttctatgatg cagctgcacc aatcgtagac aaagctacga tcgatatgga
    17881 taaagtatat ttgaagtccc gctatgacaa aggagaagca gcttacttga actgtccgat
    17941 gacagaagag gaattcaaag ctttccatga ggcactaatc aatgccgaag tggcagaact
    18001 taaatctttt gagaaagaaa aatattttga aggatgtatg ccaattgaag taatggcaca
    18061 gcgaggattc aaaacgatgc ttttcggacc tatgaaacca gttggattgg aagatcctaa
    18121 aactggcaaa cgtccttatg ctgttatcca gctgcgtcaa gacaatgctg cagcttcttt
    18181 atacaacatc gtgggctttc agactcactt gaaatggggc gagcaaaaac gtgttttccg
    18241 gatgattcct gggttagaaa atgctgaatt cgttcgctat ggtgtgatgc accgaaacag
    18301 ctttatgaat tcgccagaat tgctgaaacc gacttaccaa tcaacgaaac gcgaagatct
    18361 gttctttgca ggtcagatga ctggtgtaga aggctatgtt gaaagtgcag cgagcggact
    18421 acttgctgga atcaacgccg cacgcttagc aaaagaaatg gaaccagtcg agtttccaca
    18481 agaaacagct attggaagta tggcttacta cattactcat gcggaaggaa aacatttcca
    18541 accgatgaat gccaactatg gtctgtttcc tgaactgcca gagcgcattc gagacaaaaa
    18601 agcaagatat gaagcaattg cacaacgagc tttaaaagct aatgaggcaa taaaaacaga
    18661 attagaaaaa actgcaatcc caaatgagta aggaaactcc atgaagaggg tgagacggat
    18721 agtctcgctc tttttctggc gtttttctta agaatttctc aaacaaaatg tgaggaattg
    18781 cgccaattgt gaacaaactt agaaatattg gttaaattct gacaatttat ttgtcttttt
    18841 ataaacgctg tgctaaatta ttctctggga caaaaggaag gtgagaaaat gacagaagcc
    18901 acgtgggaac aaaaattttt gcgttatttg attgtcgaaa gaggatattc agaaaaaacg
    18961 aaagaagcat atgaagaaga cattctccat ttcaaacatt ttttggagga atctggagac
    19021 gctgatctgc tgaaggcgga atattttacg gttcgtgtct atttgagtgc attagctgat
    19081 catcgtccta gctatagcag aaattctatc agtcgcaaga tagccagcct acgttcgttt
    19141 tatcagtatc ttctcaaaga agaagtgata aaagaaaatc cattttctta cgttcatctc
    19201 aaaaagaaaa atttgagact gccacgtttt ttttatgaaa atgaaatgca agcacttttt
    19261 gacagcgtag caggagatag tcctttggat ttgaggaacc gcgctttact ggaagtcctt
    19321 tacgggtcag gcatccgttt aagtgagtgt agcagtttgt cggtatcaga cattgatttt
    19381 gattcagagg tcatgttgat ccatggtaaa gggaacaaag agagatatgc tcctctaggt
    19441 tcttttgctc aagatgctct gcaagaatat ttcagggaag gccgccaggt attgatgaca
    19501 aaatatcacg aagagcatga tatcgttttt gtgaatcatc atggaaaacc aatcacgcct
    19561 actggaattg aatatgtctt gaatcaaatg atcaaaaaaa gcagtttgga tagtgatatc
    19621 catcctcaca tgctgcgcca tacatttgct actcatttat taaataatgg ggcagatttg
    19681 cggacagtcc aagaattgct aggacatgcc aatttatcga ctacccagat ctatgcccat
    19741 gtaacaaaag aaagtttaca aaaaaactat cgttctttcc atccaagagc gtaaaaataa
    19801 ggaaaagaac a
//
LOCUS       DACEDM010000065        19457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEDM010000065 DACEDM010000000
VERSION     DACEDM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.065"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWO50_002136"
     CDS             133..3660
                     /locus_tag="IWO50_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0359242.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWO50_002137"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWO50_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0359243.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWO50_002138"
     CDS             7448..7789
                     /locus_tag="IWO50_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0359244.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWO50_002139"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWO50_002139"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0359245.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWO50_002140"
     CDS             9146..11566
                     /locus_tag="IWO50_002140"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0359246.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWO50_002141"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWO50_002141"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0359247.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWO50_002142"
     CDS             13000..13737
                     /locus_tag="IWO50_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359248.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWO50_002143"
     CDS             13751..14566
                     /locus_tag="IWO50_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0359249.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWO50_002144"
     CDS             14579..15226
                     /locus_tag="IWO50_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0359250.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWO50_002145"
     CDS             15242..15898
                     /locus_tag="IWO50_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0359251.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWO50_002146"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWO50_002146"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0359252.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWO50_002147"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWO50_002147"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0359253.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWO50_002148"
     CDS             18240..18758
                     /locus_tag="IWO50_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0359254.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWO50_002149"
     CDS             18865..19365
                     /locus_tag="IWO50_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0359255.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DACEDM010000066        18841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEDM010000066 DACEDM010000000
VERSION     DACEDM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.066"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(22..252)
                     /locus_tag="IWO50_002150"
     CDS             complement(22..252)
                     /locus_tag="IWO50_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359256.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="IWO50_002151"
     CDS             complement(688..1254)
                     /locus_tag="IWO50_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0359257.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="IWO50_002152"
     CDS             complement(1254..2210)
                     /locus_tag="IWO50_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0359258.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="IWO50_002153"
     CDS             complement(2210..2866)
                     /locus_tag="IWO50_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0359259.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="IWO50_002154"
     CDS             3236..5254
                     /locus_tag="IWO50_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0359260.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5681..8095
                     /locus_tag="IWO50_002155"
     CDS             5681..8095
                     /locus_tag="IWO50_002155"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0359261.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="IWO50_002156"
     CDS             8443..9996
                     /locus_tag="IWO50_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0359262.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="IWO50_002157"
     CDS             complement(10377..12161)
                     /locus_tag="IWO50_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359263.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="IWO50_002158"
     CDS             complement(12176..13897)
                     /locus_tag="IWO50_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359264.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="IWO50_002159"
     CDS             13997..14758
                     /locus_tag="IWO50_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0359265.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15725..15993
                     /locus_tag="IWO50_002160"
                     /pseudo
     CDS             15725..15993
                     /locus_tag="IWO50_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16665..>18841
                     /locus_tag="IWO50_002161"
     CDS             16665..>18841
                     /locus_tag="IWO50_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359266.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         6186 a         3343 c         3771 g         5541 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    15301 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat tgttttttta
    15361 tagaaatttt aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata
    15421 ttatagaagt tgactggtag tgatttattt ttcgtcgttt tataaaaatg ttattttttg
    15481 gaaaaattca caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta
    15541 tgactaatat aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg
    15601 gaatcaatga aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat
    15661 agcaatacat cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac
    15721 aacaaaagga ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt
    15781 tattctatac aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa
    15841 tatatggtac agaatataga aattattgac attacagaaa atgatgaaga tttaatacag
    15901 attgtcacta tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg
    15961 attaaggagt tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga
    16021 gcaaaaagag taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt
    16081 ttattagtaa tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg
    16141 tttagttgaa ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc
    16201 caagtattct aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt
    16261 gatgaaggtt aaccaataca gggactcact gatgaaattc tagagtctga ccttggtatc
    16321 aacgatttag tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa
    16381 taactattag ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca
    16441 taattatggt taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac
    16501 agaagacaat cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa
    16561 ccgaaaatac gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta
    16621 tcgggtacct tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc
    16681 ataaactgtt aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa
    16741 cggttctagc tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc
    16801 aagaggaggc tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc
    16861 taaatgcatt ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca
    16921 atgattacga acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg
    16981 caagaagcgg tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa
    17041 gcacaagtga gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag
    17101 aagaaagctt aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg
    17161 aatctcagat tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc
    17221 gccccacttt gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg
    17281 gcgatattct tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct
    17341 taattgagtt cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt
    17401 accaagaagg cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag
    17461 ccgctcagtc gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta
    17521 ctgagccaaa taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg
    17581 aattttcaat tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct
    17641 ctagagaaat cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga
    17701 ccaacagtaa taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag
    17761 tggttaccga tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt
    17821 ccactggaaa tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag
    17881 gtgaagcaaa cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta
    17941 ataaggaaac gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt
    18001 ttattgaata tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg
    18061 atgggttttc tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact
    18121 accgcttgag aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca
    18181 aaaatgttat caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt
    18241 ctggaacaag aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt
    18301 catttctatc agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt
    18361 tcagtgcgaa taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg
    18421 tagaggcgta tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca
    18481 atcgattgaa tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta
    18541 aaactgatga gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt
    18601 cacaagtgat acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc
    18661 ctgtaggtag ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga
    18721 cggtacatga tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa
    18781 acatccttga tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc
    18841 t
//
LOCUS       DACEDM010000067        17003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEDM010000067 DACEDM010000000
VERSION     DACEDM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.067"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..124)
                     /locus_tag="IWO50_002162"
     CDS             complement(<1..124)
                     /locus_tag="IWO50_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0359267.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="IWO50_002163"
     CDS             complement(228..539)
                     /locus_tag="IWO50_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0359268.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="IWO50_002164"
     CDS             complement(541..738)
                     /locus_tag="IWO50_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0359269.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="IWO50_002165"
     CDS             complement(1132..2859)
                     /locus_tag="IWO50_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0359270.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="IWO50_002166"
     CDS             complement(2852..4045)
                     /locus_tag="IWO50_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0359271.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="IWO50_002167"
     CDS             complement(4232..4876)
                     /locus_tag="IWO50_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0359272.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="IWO50_002168"
     CDS             complement(4970..5104)
                     /locus_tag="IWO50_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0359273.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IWO50_002169"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IWO50_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0359274.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="IWO50_002170"
     CDS             complement(7235..7708)
                     /locus_tag="IWO50_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0359275.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="IWO50_002171"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="IWO50_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0359276.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="IWO50_002172"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="IWO50_002172"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0359277.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="IWO50_002173"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="IWO50_002173"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0359278.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="IWO50_002174"
     CDS             complement(11755..12894)
                     /locus_tag="IWO50_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0359279.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="IWO50_002175"
     CDS             complement(13203..13916)
                     /locus_tag="IWO50_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0359280.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IWO50_002176"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IWO50_002176"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0359281.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="IWO50_002177"
     CDS             15105..16637
                     /locus_tag="IWO50_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0359282.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17003)
                     /locus_tag="IWO50_002178"
     CDS             complement(16828..>17003)
                     /locus_tag="IWO50_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0359283.1"
                     /translation="FMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQT
                     VVFKGKRNLENKK"
BASE COUNT         5277 a         3494 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ata
//
LOCUS       DACEDM010000068        16271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEDM010000068 DACEDM010000000
VERSION     DACEDM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.068"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(56..712)
                     /locus_tag="IWO50_002179"
     CDS             complement(56..712)
                     /locus_tag="IWO50_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0359284.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            905..1183
                     /locus_tag="IWO50_002180"
     CDS             905..1183
                     /locus_tag="IWO50_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359285.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1202..2410
                     /locus_tag="IWO50_002181"
     CDS             1202..2410
                     /locus_tag="IWO50_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0359286.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2558..2923)
                     /locus_tag="IWO50_002182"
     CDS             complement(2558..2923)
                     /locus_tag="IWO50_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0359287.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3070..3843)
                     /locus_tag="IWO50_002183"
     CDS             complement(3070..3843)
                     /locus_tag="IWO50_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0359288.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4017..4877)
                     /locus_tag="IWO50_002184"
     CDS             complement(4017..4877)
                     /locus_tag="IWO50_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0359289.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4877..6295)
                     /locus_tag="IWO50_002185"
     CDS             complement(4877..6295)
                     /locus_tag="IWO50_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0359290.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6352..6801)
                     /locus_tag="IWO50_002186"
     CDS             complement(6352..6801)
                     /locus_tag="IWO50_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0359291.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7246..7509)
                     /locus_tag="IWO50_002187"
     CDS             complement(7246..7509)
                     /locus_tag="IWO50_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0359292.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7608..8903)
                     /locus_tag="IWO50_002188"
     CDS             complement(7608..8903)
                     /locus_tag="IWO50_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0359293.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8900..10171)
                     /locus_tag="IWO50_002189"
     CDS             complement(8900..10171)
                     /locus_tag="IWO50_002189"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0359294.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10336..11205)
                     /locus_tag="IWO50_002190"
     CDS             complement(10336..11205)
                     /locus_tag="IWO50_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0359295.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11497..13107)
                     /locus_tag="IWO50_002191"
     CDS             complement(11497..13107)
                     /locus_tag="IWO50_002191"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0359296.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="IWO50_002192"
     CDS             complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="IWO50_002192"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0359297.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15668..16060)
                     /locus_tag="IWO50_002193"
     CDS             complement(15668..16060)
                     /locus_tag="IWO50_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0359298.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4639 a         3449 c         2766 g         5417 t
ORIGIN      
        1 cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt gttcatcaaa
       61 agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg tctttaacgg
      121 tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta gcagttgaga
      181 tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc atcccttctt
      241 ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca gctcccagct
      301 ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga tagcggtaat
      361 actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa aagcgcacac
      421 gattgattcg ataaaactct gcatattctg attcaactcg aatattatac ggcgaatcga
      481 tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg atatagcgaa
      541 ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc tctttcaaga
      601 tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat cctttgcgta
      661 taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc aatgatgctc
      721 ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt taaagtccaa
      781 aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac ttatgcgatt
      841 tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa tagagggaaa
      901 aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat atcaaacaag
      961 atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact cgatcgaatt
     1021 gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa atctgtttat
     1081 gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca attatcacga
     1141 tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat gtataacata
     1201 catggaaaag aaattattta ataaatattt tattggaatc acattgatca attttattgt
     1261 ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag acaccttaca
     1321 cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa cattgctggc
     1381 acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct taagaggcgg
     1441 cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta tcggggcatt
     1501 gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag ctacaaatgc
     1561 tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt attatggatt
     1621 aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc tgaatatgac
     1681 tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta cgattgcctg
     1741 ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat ctcttcgctc
     1801 atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa ttgccttttt
     1861 gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa aagtaatcca
     1921 cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca catttacacg
     1981 tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt atcctagcta
     2041 tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg tgacattgct
     2101 tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg gacaggctgt
     2161 ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct actttattgg
     2221 tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat ctcttacttc
     2281 atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag cactctatct
     2341 tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat cattgaaaaa
     2401 tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa taatctgagc
     2461 ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt tatttttaaa
     2521 gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc cgttcatctg
     2581 gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca ccgatcaata
     2641 gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa tatagataga
     2701 gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg ttccctactt
     2761 tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt accccaacaa
     2821 tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac agaatcgccc
     2881 agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc actcctttct
     2941 cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat accatcggct
     3001 gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt agaacattca
     3061 cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg catactgttc
     3121 ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa tggcttgatg
     3181 catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt ctgattgttt
     3241 tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc cttggctgaa
     3301 atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg catcgttata
     3361 ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca atacagatac
     3421 ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg taatgatctg
     3481 caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac tggaatccaa
     3541 gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct tacgtttttc
     3601 tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt ttcgattgtc
     3661 aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca gaaaaacaag
     3721 gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg tgactcttgc
     3781 aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca cttcattcaa
     3841 cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt gacagaagtg
     3901 attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac atcgtgtgtg
     3961 gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt ttatttttag
     4021 tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac cacttcttgt
     4081 acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg attttcactc
     4141 aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa attcactttg
     4201 gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga accgccatgt
     4261 aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat atttaatttt
     4321 ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc aagtgtatcg
     4381 attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat gattgtttca
     4441 gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc cactgagaca
     4501 tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc gtcgaatgat
     4561 ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg taagacatct
     4621 tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt ataagcatag
     4681 gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg atgaatttct
     4741 aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat catatcatag
     4801 ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt caataactct
     4861 ttcattgtat ataacattac gcttccacct catttgttag attttcattt ttcgtccact
     4921 tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag gctgtagtga
     4981 ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca tttggaatta
     5041 gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc atggaaatcc
     5101 ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg tcagataccg
     5161 caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat gattgacctg
     5221 aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa gcaataccta
     5281 ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact ccagctgaca
     5341 tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata tcagctgcca
     5401 tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag ctattcagcc
     5461 aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat accatcaaaa
     5521 gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata gcagaaaatt
     5581 ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct agcacgccac
     5641 caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg gcaacaattc
     5701 ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct aacgcaggaa
     5761 ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct aacacattat
     5821 agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt gcgatgatga
     5881 ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa tgattataga
     5941 ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg aatacgccaa
     6001 tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg cttgtagaga
     6061 cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct actgcaagaa
     6121 tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt tttacacctt
     6181 gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat ttttctgccg
     6241 acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt ttcatgatta
     6301 ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt ttcattcttg
     6361 actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct ttctaaaatc
     6421 tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat ctgctgattt
     6481 ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc cgctatagtc
     6541 cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt tgctttttgc
     6601 atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc gtttgaatag
     6661 tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca catcccctat
     6721 tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca gttcttctgc
     6781 gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta acgttttctt
     6841 ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa tgaacagagg
     6901 aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc tcacaaacaa
     6961 tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc ttctaatttt
     7021 aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat aaggtgtttt
     7081 tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca ttatgatcct
     7141 acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa aaacagacta
     7201 tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt ccgcagcgtt
     7261 tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg cttgtgtgaa
     7321 tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac ggatgactgg
     7381 gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg aacctgacaa
     7441 gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg gatggatgtt
     7501 ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag agttgatttg
     7561 tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta tcttttcaga
     7621 tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa aaattgctga
     7681 ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa tgaatctcca
     7741 gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat caataattct
     7801 tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg ttcagataat
     7861 tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata gatcacgtca
     7921 tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact gccaccttct
     7981 tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc tgggtcaatc
     8041 ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg agcaagtcta
     8101 gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg cattgcgcgt
     8161 tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa tgtagaagat
     8221 accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg ttcatcaatc
     8281 agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc gatttctttt
     8341 aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg accaaaaccg
     8401 attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt ttccagttga
     8461 agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc ttttgcttct
     8521 tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg ttccgattct
     8581 ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa tcgtctgatt
     8641 tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg ccgtaaaaaa
     8701 ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa tccttgtttc
     8761 tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt actgtaataa
     8821 ggaattttaa attctttggc ataactataa acttctttta acgttttgtt ctctagttct
     8881 gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca attgatgccc
     8941 ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa tctacattgt
     9001 aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca cctgcccgaa
     9061 gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg atgatcatat
     9121 ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg ttcacacgtt
     9181 tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt aacgcagtaa
     9241 gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg tttgcgggtt
     9301 ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata tggacaccaa
     9361 tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt ttcactttga
     9421 taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg cggtcaggaa
     9481 tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc acatctgtac
     9541 ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct atgatttccg
     9601 gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca gctaacatca
     9661 cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca cgcaatcctt
     9721 tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca cctagagctt
     9781 caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca cctggcaggc
     9841 ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa gaagcacgca
     9901 aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga tcgatttcta
     9961 gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca attaatgaat
    10021 gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct aagattgcag
    10081 ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca ccagctaaag
    10141 gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat taatcttttt
    10201 tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg gtctgccgtt
    10261 ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta tatatcgtat
    10321 ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat gagatttagc
    10381 atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga atcctttacc
    10441 ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg ataattggaa
    10501 ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga tttgatcttg
    10561 aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag catcagcaat
    10621 tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac catggatgtt
    10681 accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc attcttcaat
    10741 atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg aaccaacttc
    10801 acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag ctaatttcaa
    10861 gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc ctaattcgat
    10921 acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg gaacagtgat
    10981 gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac cacccatgta
    11041 tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt cagccgcttc
    11101 aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag cgtaaccgcc
    11161 tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa aattcctcct
    11221 aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta ttttaccaaa
    11281 catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa gttttttgca
    11341 aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc tatctttttt
    11401 cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc ttatcccctt
    11461 ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt gatctgctaa
    11521 tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag aaatcaattc
    11581 tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga tttctactaa
    11641 acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca attgacgata
    11701 tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg ctccatcata
    11761 tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa ggcgtaaagt
    11821 tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat tgtttgctgt
    11881 atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac gtgcaaattc
    11941 gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat tttcgcgagc
    12001 ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac caggaaccaa
    12061 gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt cttgtgcttt
    12121 gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca atgcttcaac
    12181 tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa tccgtacttt
    12241 tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt ctgcttctgg
    12301 tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt gtaaaactaa
    12361 tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg gttcgacatc
    12421 acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg tacgtaccac
    12481 caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct gcgttggttt
    12541 agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga tatacataac
    12601 attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg gcaaagattc
    12661 tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag tcattttagc
    12721 tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt gaacagttgc
    12781 tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt ttcctgtcgt
    12841 cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc ccagatccaa
    12901 atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg ggctcatcgt
    12961 ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta agccgcggtt
    13021 tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg aaacgacacc
    13081 gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat ttatgtgcag
    13141 gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg gagccgaaat
    13201 ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa gctgactaag
    13261 ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt tcgatatcct
    13321 ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc acataataag
    13381 cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt actgaaaaat
    13441 tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa tatcccacct
    13501 aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc gtagaaacaa
    13561 tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca cagttctcgg
    13621 atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc actaattttt
    13681 tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa ggtaacgaat
    13741 cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt ctaaataaac
    13801 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca tgagaagctg
    13861 tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc tctggataaa
    13921 cggtattcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagcc
    13981 attttcacaa aatttttctt aatactcgaa actaaacgtc tttttcaaaa cctcttggta
    14041 aacggtgttc aaaagcttgc atctgcggta ataagttcag ccaattcaaa aacaagaaaa
    14101 gctgtttttg cttggctatt cttattactt gttgcagggc agctttttca caacctctcc
    14161 actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    14221 gaactatttt tccgttagtt tctctaatac tcaggtcaaa acgctgattt cacagcctct
    14281 aattcacggt attcctgttt taataaagtt ctagatattg ttctctttcc cactcggaaa
    14341 cagtttgtct gaatgccgcc cattcgagac gttttgcttc aacaaaattt gagaagatat
    14401 gttgtcctaa tgcgtcaatc attacattat ccttacgcaa ttctttgata gcattatgga
    14461 tagttgaagg taagtcctgg atatgtgcag cttctctttc ttcctcatcc attacataaa
    14521 tgttacggtc aacagctgct ggcggtgtga tctgattacg gataccgtct agtcctgctt
    14581 gcaacaatac tgccattgcc aagtatgggt ttgcagatgg atcaactgac cgcaattcca
    14641 aacgtgtgga taaaccgcga gattctggta cacgtactaa tggcgaacga ttacggccac
    14701 tccatgcgac ataaacaggc gcttcataac ctggaacgag acgtttataa gaattgactg
    14761 ttggattgca aacggctgtg taggcacgag catgtttcag caaaccacct aagaaatgat
    14821 aagctgtttc acttagttcc aattcaccgt ctttatcata gaaaacattt ccttcatcat
    14881 tgaacaaaga catattgcaa tgcataccag aaccattgat cccaaataac ggtttcggca
    14941 tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt gacaacgagc ttgaaggttt
    15001 ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca tgctgtcctg
    15061 gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat tccaacacga
    15121 tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa ccacctttat
    15181 cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag aattctggtt
    15241 caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat gctcgtttca
    15301 aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata tcacaaatta
    15361 aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg gagagatctg
    15421 gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa gaaccgtcaa
    15481 acatcatttt attttctaat actttgtcga tctggctcac tggaacttct acatttttga
    15541 tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct tcctcaatga
    15601 ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc ttcctttttt
    15661 aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga cctgttttgc
    15721 ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca tgatctgtca
    15781 acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat acacgtttga
    15841 tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac atatcaatat
    15901 cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact aaatcttgtt
    15961 cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact gtaccaattg
    16021 gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc ctttcgtttt
    16081 ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat gttaggtttt
    16141 ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca aaaaaggtat
    16201 ctagttgatt ttcgtaccag atacctttta ttttttatta tactgtacta tcctttatta
    16261 gtgtttttat t
//
LOCUS       DACEDM010000069        16100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEDM010000069 DACEDM010000000
VERSION     DACEDM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.069"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..357
                     /locus_tag="IWO50_002194"
     CDS             <1..357
                     /locus_tag="IWO50_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0359299.1"
                     /translation="DSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTT
                     SDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILL
                     VIGLRITFMSYKKSKR"
     gene            complement(512..1498)
                     /locus_tag="IWO50_002195"
     CDS             complement(512..1498)
                     /locus_tag="IWO50_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0359300.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1521..2087)
                     /locus_tag="IWO50_002196"
     CDS             complement(1521..2087)
                     /locus_tag="IWO50_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0359301.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2101..2517)
                     /locus_tag="IWO50_002197"
     CDS             complement(2101..2517)
                     /locus_tag="IWO50_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0359302.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2974..4908)
                     /locus_tag="IWO50_002198"
     CDS             complement(2974..4908)
                     /locus_tag="IWO50_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0359303.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5210..6145
                     /locus_tag="IWO50_002199"
     CDS             5210..6145
                     /locus_tag="IWO50_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0359304.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6235..7338
                     /locus_tag="IWO50_002200"
     CDS             6235..7338
                     /locus_tag="IWO50_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0359305.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7351..8304
                     /gene="menA"
                     /locus_tag="IWO50_002201"
     CDS             7351..8304
                     /gene="menA"
                     /locus_tag="IWO50_002201"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0359306.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9118..10464)
                     /gene="gor"
                     /locus_tag="IWO50_002202"
     CDS             complement(9118..10464)
                     /gene="gor"
                     /locus_tag="IWO50_002202"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0359307.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10598..11839)
                     /locus_tag="IWO50_002203"
     CDS             complement(10598..11839)
                     /locus_tag="IWO50_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0359308.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11857..12780)
                     /locus_tag="IWO50_002204"
     CDS             complement(11857..12780)
                     /locus_tag="IWO50_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0359309.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13001..15493)
                     /locus_tag="IWO50_002205"
     CDS             complement(13001..15493)
                     /locus_tag="IWO50_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0359310.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15500..15967)
                     /locus_tag="IWO50_002206"
     CDS             complement(15500..15967)
                     /locus_tag="IWO50_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0359311.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4724 a         3360 c         2863 g         5153 t
ORIGIN      
        1 gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
       61 acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg
      121 tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat
      181 aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa
      241 agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata
      301 ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa
      361 aatattcaag ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt
      421 tatcataaat acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag
      481 aaatttattt acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg
      541 taagggaaaa gagattctct tttgttcgaa gagaggtggc aggtaatttc tttatttcct
      601 tcaaagcctt ttctgtgtaa gaggccgcta acttcctagc ttcttccacg cctcctgtat
      661 gttcaacgat tttgcgtacg gcgtcgacat cttcttctgt cattgccgct ttcttctcta
      721 aataaggtag tagctttggt ttgttttttt gcaaagagta gatcaacggt aaagaataga
      781 ccccttgacg catgtcttct agtacaggtt tgccgatttc ctggctctct tttgtataat
      841 ctaaaatatc atcgataatc tgaaaggcca tcccgataga aagtccgata tctcctgctt
      901 tttttgcgaa gcgctcgctt gttccacttt caaaagcgcc aacagaacaa cttaattgga
      961 acagttcagc tgtcttacct gaaatatttt ccaaatattc ttttacagaa agttcataat
     1021 tatagcgatc atccatctgc cctaattcgc cattcaatac tttttccata ctgcgagaat
     1081 tcaattggag acttttcaag gaagtggagt aatcagacaa tagtttaaaa cagcagacaa
     1141 ataaatagtc tcctgcatag acagaaacga cattaccaaa ctgactacgg atattaggct
     1201 gaccacgtcg cagatccgct tcatcaacga tatcatcatg gatcagtgtg gccgtatgga
     1261 gcatttcgat agaagctgcc aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt
     1321 gtgagaacaa tagctgatag gcaggacgca acagctttcc gccggcatgg atcgtttctt
     1381 tgattgcttg ttcgacaggt ttgtttttca ggcggacagt ttcttccatc cgttttagtg
     1441 tttctgtcaa ttctttactt aaagcaggat agttttccca cattggatgt agcttcataa
     1501 aaaaactcct tgtgtgacga ttattttttg gtaaacattg ggctccattt ttgtttggtt
     1561 ccctgctcat aatctttttg gaatcgttga agtgtctgca catgtcgtaa aagataattc
     1621 gctgcgatcc cgattgcaat ccctgataaa atccctaaaa ctgaaaggac tggcaagtac
     1681 aacatgactg tccatgattg tgcaatgaaa gaagccatca aaagctgtcc gacattatgc
     1741 atgaaaccgc cggccgcact aattccgatg atactgacac gttttggtcc taattgcttg
     1801 accaataaca ttccaaaata actaagcaaa gcaccactca tactataaag gaatgtggaa
     1861 atcgttccgc ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct
     1921 cgttttttca tggtaaaaat ggcaacgatc gtgattagat tggctaagcc tagttttgca
     1981 ccaggcgcaa aagaaaaagg ataagggatc atattttcca ataaaccaat gatcactcct
     2041 tgtgcaacaa gtagcgaaat ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt
     2101 ctaataaatc agactgtctt cgtcacctcc atctgttgaa cggacttcga tcactaattt
     2161 atgtggcaaa cagacgatgg tttcgccatt tttcgttgcc cagcctcgac gtacgcagat
     2221 ctggtcatcg caatctgctt cttttatccg tactcgatcg ccatctatct cgatcaagtt
     2281 ataatctccg tgttcatctt tgtaagtata tgtgttgctc tcttctccag ccatcaaagg
     2341 gaatgttttg atttctgtgc catccacacg cagtaccgct tctttttctg ctgttgcggt
     2401 gttttgctga agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat
     2461 aataaaaaca tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt
     2521 tgttctcctt tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca
     2581 cctgtgtctt gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta
     2641 attctttggg cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt
     2701 gacagaagtg ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt
     2761 ttgtgaaatt tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga
     2821 ggttgtaaca gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt
     2881 attcctttaa ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg
     2941 aaatcttgca ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg
     3001 tctaccgtac caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa
     3061 cccaatcgca cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt
     3121 tacccagtaa aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact
     3181 gcatagccat gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac
     3241 catgaatttt tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt
     3301 tgtgaaccag tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact
     3361 caaaccaaaa tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc
     3421 agctgtggtt gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc
     3481 ccatgtccaa gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact
     3541 tggtttgaac acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa
     3601 accatagaaa atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc
     3661 tcgagtcacg ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat
     3721 ataataacca gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat
     3781 agctaaaaat ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc
     3841 aatagaaacc ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat
     3901 cgaagccacg atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg
     3961 cggtgtcggt gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt
     4021 tttatcttcg tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc
     4081 tgattctaat ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag
     4141 tgtgtgagtc ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac
     4201 gattggtgca ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc
     4261 agcatcattt cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt
     4321 gatttcgttt ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc
     4381 cccaatcatt tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt
     4441 acgcagttca tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg
     4501 aatccttaca gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt
     4561 accaaagtca ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc
     4621 taatttggtc ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag
     4681 ttgtacattc ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc
     4741 tacacgtccg ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc
     4801 gatcagagta attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga
     4861 aacaccggca tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct
     4921 ccttatagac tatctatata atgaatatat gataattctt ttagtttgat acttattaca
     4981 caatctccat atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt
     5041 caaattttaa aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt
     5101 acatgggtta tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg
     5161 aatcacaaag aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa
     5221 acgttttgta actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg
     5281 cgcagatgac aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac
     5341 tgcaaaaagc acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg
     5401 cacatataaa ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt
     5461 tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc
     5521 aaaaacaaaa gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga
     5581 aaaatacatt cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga
     5641 agtcgtaact ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat
     5701 ccaagctgct caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa
     5761 agacggcact tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga
     5821 aatgacagtt gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg
     5881 taaatcaaaa caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga
     5941 accaaaaact tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga
     6001 tggctcacct gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat
     6061 catgtacgca caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt
     6121 agataatatc gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg
     6181 acgatagcca gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg
     6241 aaaaaactac tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc
     6301 agcaactcag caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat
     6361 ccatattctg atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa
     6421 ggcaaagaat cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc
     6481 acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca
     6541 gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat
     6601 tctcaaggcg gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt
     6661 gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat
     6721 cataagatcg aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc
     6781 atcgatttag gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa
     6841 aaacaaggag tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac
     6901 agcccaagag gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc
     6961 ggatctgttt taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac
     7021 gagcgttatt tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt
     7081 tatccgttcg acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt
     7141 gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa
     7201 agcgaattga ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg
     7261 accgatccaa tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac
     7321 cgttctgaac taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt
     7381 gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc
     7441 gctttttttc atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt
     7501 tttgatttag cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa
     7561 gtatataagt atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga
     7621 aacatgattt tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat
     7681 acgggttggc ttttactttt gatgggattt atctgttgtt ttattggagt attctataca
     7741 ttcggaccga ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg
     7801 ggccttggaa tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat
     7861 ctgacagtca acttttcatc tggacaattt gctttggtag gaaatctttg ggctatagca
     7921 gcaatcgtat tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat
     7981 aatttgcggg atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc
     8041 ggacgtcctg cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt
     8101 ttaatggggt tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg
     8161 ccaattgtga agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc
     8221 agctattcca tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta
     8281 acaatcatta tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc
     8341 aagtaataag aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat
     8401 taccgcagat gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt
     8461 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
     8521 taataccgag aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg
     8581 tcctgagtat tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc
     8641 taataccgaa ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca
     8701 caattttttt cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag
     8761 aactttcttg gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa
     8821 tttatttata caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt
     8881 ttaaacatcg ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc
     8941 ttcgtcagtt acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat
     9001 tatcgaagca atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga
     9061 ttttctggtt ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta
     9121 tcgcatcgtt acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc
     9181 ttctttagtt gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc
     9241 aacaccaata gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat
     9301 ttcgcatttt tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata
     9361 aactttcaat tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt
     9421 tgcaattggt ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc
     9481 tttctttcca ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt
     9541 cagatctaat ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg
     9601 gtatttatct actttgataa accctttttt gtccaattcc acatttgtat tttccagacc
     9661 taatttttcg gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt
     9721 ttcaccattt tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat
     9781 atgatgtgcg taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag
     9841 cattttatca aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc
     9901 taaaccacga aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc
     9961 tctcttaggt aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc
    10021 aggaatggtc attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata
    10081 ggtttcaccg tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc
    10141 ctcgacatga ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt
    10201 ttctcgtctt tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta
    10261 acttgaggca tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt
    10321 aggtacacaa ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac
    10381 ttttgcgcca tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat
    10441 tacgatgtaa tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct
    10501 ttatttaacg atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg
    10561 cgaagatgct taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat
    10621 ctccaggttt caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc
    10681 ggtcaatgga ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga
    10741 atcctggacc ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg
    10801 tagcaatctg tgttgcctca tcataagata atacttcacc gacaaattta tatactggga
    10861 ttttacgacg ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg
    10921 tagctaattc tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat
    10981 agttttcagg atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag
    11041 atacataatg gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa
    11101 tcaattctgg aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt
    11161 ccacttcgcc tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc
    11221 catacttcca gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat
    11281 ggttggacaa tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt
    11341 cgatttcggt atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag
    11401 ctaaaacgac acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg
    11461 ctgaagcttg agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga
    11521 tcaaagctgg atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa
    11581 attcccctgc tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct
    11641 tcgcatcatg ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac
    11701 ctgcacggct tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat
    11761 ggatcgcggt ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca
    11821 attttctttt tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat
    11881 ataaaagaat cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt
    11941 cattttttcc gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc
    12001 gaatagtcga gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca
    12061 tgtacagtaa tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct
    12121 ggcaaaaata tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt
    12181 aggttccgag ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt
    12241 atagtaattc tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag
    12301 ggtttctaca ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc
    12361 acgagcaagt actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct
    12421 cgttactaat gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat
    12481 cgtataaata atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa
    12541 agccaaatat tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat
    12601 attcgctttt ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt
    12661 aaatgaagca ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc
    12721 tgcagcaagc aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat
    12781 aattttctcc tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc
    12841 ttctatttaa agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata
    12901 tattctgtcc aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa
    12961 gtcttactat tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt
    13021 cctttttagg tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt
    13081 ggtcacctaa acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt
    13141 tttgaagtgc gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga
    13201 tcacatcgat tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga
    13261 taatcgcttt gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga
    13321 agacgacagt ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt
    13381 ctaagatacg tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga
    13441 catttttttc ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat
    13501 ttctaaagtc cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca
    13561 gaagtaaatt gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat
    13621 atggtttttg acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg
    13681 gaggagaccc gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac
    13741 ggatcagggc atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt
    13801 ttccgacacc agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca
    13861 atccgcttcg tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa
    13921 cgacacgttc atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca
    13981 actgctgtag agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta
    14041 cacgatcaga ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt
    14101 tacgtagtgc tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga
    14161 tcgctgcttc cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg
    14221 ttaagtgatc ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg
    14281 ctttatcggg tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta
    14341 cagcttcatc tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa
    14401 gaatcacttc tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct
    14461 ctaatgcaga atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg
    14521 tttgtaattc tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt
    14581 ctgctccacc tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat
    14641 cattatagat ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg
    14701 taccagcaac caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg
    14761 gcacttcacc taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac
    14821 caggttcacc caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc
    14881 gtttcacttc tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg
    14941 tcaaatcacg tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt
    15001 gtacatttac atttctacga cgattcattc cttttccagc agaatcacca gctagcccca
    15061 ttttttgttt cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc
    15121 gagtagccat gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt
    15181 tttgagaacc agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg
    15241 gtgaatatgg aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat
    15301 gttcaatttc ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag
    15361 caattccatt ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat
    15421 gtttcaaccg ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa
    15481 aaagttcttc catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc
    15541 ggagaatccg agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa
    15601 ttgcactcaa catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt
    15661 gaataatagc aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct
    15721 caagtaattg ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac
    15781 cacgtttgct ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt
    15841 tgatttgtga aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca
    15901 tttcactatt ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat
    15961 gtgccatctt attcattccc tttgactaat attgacctta attataacga atttgataat
    16021 agaaagaaag aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac
    16081 ggaatatact attccgtatc
//
LOCUS       DACEDM010000070        16047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEDM010000070 DACEDM010000000
VERSION     DACEDM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.070"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(68..994)
                     /gene="glsA"
                     /locus_tag="IWO50_002207"
     CDS             complement(68..994)
                     /gene="glsA"
                     /locus_tag="IWO50_002207"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0359312.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1017..2228)
                     /locus_tag="IWO50_002208"
                     /pseudo
     CDS             complement(1017..2228)
                     /locus_tag="IWO50_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2263..3876)
                     /locus_tag="IWO50_002209"
     CDS             complement(2263..3876)
                     /locus_tag="IWO50_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0359313.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(3988..4197)
                     /locus_tag="IWO50_002210"
     CDS             complement(3988..4197)
                     /locus_tag="IWO50_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0359314.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            4288..4476
                     /locus_tag="IWO50_002211"
     CDS             4288..4476
                     /locus_tag="IWO50_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359315.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            complement(5186..6400)
                     /locus_tag="IWO50_002212"
     CDS             complement(5186..6400)
                     /locus_tag="IWO50_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0359316.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(6528..6998)
                     /locus_tag="IWO50_002213"
     CDS             complement(6528..6998)
                     /locus_tag="IWO50_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0359317.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(6995..7237)
                     /locus_tag="IWO50_002214"
     CDS             complement(6995..7237)
                     /locus_tag="IWO50_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ0359318.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(7783..8313)
                     /locus_tag="IWO50_002215"
     CDS             complement(7783..8313)
                     /locus_tag="IWO50_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0359319.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(8495..9151)
                     /locus_tag="IWO50_002216"
     CDS             complement(8495..9151)
                     /locus_tag="IWO50_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0359320.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(9278..9751)
                     /gene="dfrF"
                     /locus_tag="IWO50_002217"
     CDS             complement(9278..9751)
                     /gene="dfrF"
                     /locus_tag="IWO50_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0359321.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(10170..10388)
                     /locus_tag="IWO50_002218"
                     /pseudo
     CDS             complement(10170..10388)
                     /locus_tag="IWO50_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10419..11633)
                     /locus_tag="IWO50_002219"
     CDS             complement(10419..11633)
                     /locus_tag="IWO50_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0359322.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(11974..12216)
                     /locus_tag="IWO50_002220"
     CDS             complement(11974..12216)
                     /locus_tag="IWO50_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0359323.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(12248..12805)
                     /locus_tag="IWO50_002221"
     CDS             complement(12248..12805)
                     /locus_tag="IWO50_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0359324.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(12818..13006)
                     /locus_tag="IWO50_002222"
     CDS             complement(12818..13006)
                     /locus_tag="IWO50_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0359325.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(13019..13585)
                     /gene="amaP"
                     /locus_tag="IWO50_002223"
     CDS             complement(13019..13585)
                     /gene="amaP"
                     /locus_tag="IWO50_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0359326.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            14815..15288
                     /locus_tag="IWO50_002224"
     CDS             14815..15288
                     /locus_tag="IWO50_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0359327.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            15297..15524
                     /locus_tag="IWO50_002225"
     CDS             15297..15524
                     /locus_tag="IWO50_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0359328.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         4735 a         3286 c         2470 g         5556 t
ORIGIN      
        1 aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca
       61 agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga
      121 taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac
      181 gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat
      241 ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga
      301 ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg
      361 ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga
      421 aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt
      481 aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca
      541 atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt
      601 catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag
      661 gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac
      721 cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta
      781 ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc
      841 cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca
      901 cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt
      961 ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat
     1021 ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg
     1081 ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta
     1141 gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca
     1201 acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct
     1261 ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca
     1321 acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag
     1381 acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg
     1441 tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt
     1501 gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga
     1561 atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct
     1621 aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg
     1681 gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat
     1741 ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc
     1801 atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga
     1861 atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc
     1921 cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca
     1981 atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc
     2041 aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag
     2101 gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat
     2161 tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag
     2221 acaccgccgg tttcccttag ttaaaaatat tactcttgtt gattactcaa ctcttccaat
     2281 aaatcggtaa tatatctcaa tttcctgtat tctttcgtta tcctcattcg ttgttgcttc
     2341 atgtatgagg attttttcta tcatggcatt taataatggt gctgtcagtt cctttggtac
     2401 ggaatattcc ttgattaact caatccactt ttcagcacct atcatatcct gttccatttt
     2461 acttagttct tctcttaggc ttgttatctg ctgttccagt tctatctgtt ctttttggta
     2521 cttgctggac aacatcacaa aatttcgctc tgttatcttc tcacaggctc tatcttcata
     2581 cattttcgca aataaacggt caatctcatt ttgtctgttc tcggctttct tcaatgtact
     2641 tgcctttttc ttcttttccc gtattctctc tgcattgcca gctttctgta tcttgttcaa
     2701 taccttttct tcgtcctgct gtactgcctt agcccaatac tgcaagcgtt ccaatacggc
     2761 ttgatacagc acatcataac gaatgtagtg catagaacaa ttacctttgc caaactgccc
     2821 gtagtaactg caagcataat aactgtaagg cgttttattt gtcttattcg ttccaaatct
     2881 catagaccaa ccacaatccg cacacttgac aagccctgca aaaatcggcg ttgccttttc
     2941 ctttgtctgt ctgcgtcttg attttatctg ctcctgcaca cgatagaaca cttccttgtc
     3001 aataatcggc tcgtgagtgt tttctactcg aaaccattca ctttcgggct tacgcacttt
     3061 ctttttgctc ttgaacgata ccgtagactg catattgtga acactgtttc cgatataaac
     3121 ctcacttttc aatatcgcct tgacatgagc aatcgtccac tcataacgct tactttcggg
     3181 ttttccctca aagatatgtg caaaagtacc gtatcttgta aaatttagcc atgacgctgt
     3241 cggaactttt tctgctctta actgctttgt gatttttgca cttccgtaac cttgataggc
     3301 tagggaaaag attttttcaa taatccattt tgtttcatca tcaaccaaca gttttccttt
     3361 gatgtcaggg tgtttcttat atcctaacgg agcataagca ccgtaatgag ccccctccgc
     3421 aaactttgtc ttaaatgctg atttgacttt acggcttgta tctcttgcca cccattcatt
     3481 gatgatgttc ttaaatggtg caatctcact ttcgcctttt tcgctgtcta caccgtcatc
     3541 aatcgctatg tagcggacat tatgactagg gaaatacaat tctgtatatt gtcctgtcat
     3601 aatataattt ctgccaagac gggaaaggtc tttcgttaca acacagttga tttttcctgc
     3661 ctctatatcc tcaatcattc tttgaaaact cggtctgtca aaatttgttc ccgaccagcc
     3721 gtcatcaata tattcatcaa tgacattcaa atgatgttct tttgcatata aacgcaacat
     3781 acttctctgt gtggtaatgc tggaactttc gccctgtaat tcatcatctc ggcttaacct
     3841 aagataaagt gcagtattgt aaatctgttg tttcattgtc agttatcctc ctttataact
     3901 aacccgtgtt tacaatacct gcttgcaagt aaagtataac acgggtactt tttgtcattc
     3961 aatctatttc ttacaactgc accgatttta tgcgattgtc tgctttgctt tttccagcat
     4021 gacatcagtt agaagttctt ccattgtttt gcccttttcg gaaaaatgct cttttactac
     4081 aatctttgtc tttcctctgt tgccaatgcc acacttgcca tttttctttt gaattgtttt
     4141 tacagcatta tcaataacac ataccccctt tctgtaaaat ctttctaagc aattcaacgg
     4201 cttttgtcat agcccacagg agttccacct ctgcatagtt cttatgtagc cgtacccatt
     4261 gcgtgcgacg ctcttaatgc tcaagctgtg gctgtacggg agtatcatta tctgacagta
     4321 caggtcatgg cgataaaaga ggacaaatct tttacaagaa cacaaatagg ttttcgctcg
     4381 ctcttgcagg ggaaaggtcg tggcgtattt gtttcaacgg ctagctgtct gtcaaacgta
     4441 ttgaatagct ttattcagtt gtcaaagaac gattgaaaga aaaaaatcgt ctttcctata
     4501 tatcgcgttg gaaaagagga gaaacgtaac caaaatccaa aactttttcc attattagta
     4561 caaatggaac ggttcatttt ggcaatcaac ggttgatttt ctcctttttc actactatct
     4621 gtacagcaaa cgtccatttg ctaagtttaa atggaaaaat tttaacttct tttcatgcac
     4681 catataggga tagaaaatgt cattttgcta agttgatgat gaaaaaagaa tattttcctt
     4741 ttcacaccct aactacccaa accataccgt tactgccaac aatagaatga aatattttct
     4801 ttgcaccata tagggaacgg aaaccttaaa ttgttaagtt gagaacaaag tattttcatc
     4861 ttttcacatc aacactcaac tgctaatgcc tgtttgaata caaaatgatg atgtttttcc
     4921 tcttttcaca gaagactgtc cactaatcac gaatttccgt cattttttca aaactttttt
     4981 cttctttcac ttcatatagg gacagcaagc cccgatttaa tgacctattt tcaaaaaaag
     5041 tttagatttc cttttctcgc accatatctg tacgcaagta gccattttgt tgcagattta
     5101 ataaaaaatt tatttttctt ctttcacttc acatagggaa tgaaaacagc tctttgctga
     5161 ctaaatctga aatttttcaa ttacattaaa aaataccgtc attctcttgt ggaacagcgg
     5221 tatctatacg ttcttctatg gttgatttct ctaattgtaa taaatgttta tgcttgtctt
     5281 ttagttctgc ctgctcaatc atatctttca gtatcgttgt acgattttct ctgtctaatg
     5341 cctgtaccat ttttatggtt ggtgcgactt gtctttgtag ccaatgcaac gctttttgta
     5401 aagtgtaagg ctctggcttt gtggttaagc gtatcggctc tctgttttcc cctacaaacc
     5461 atgcccaatc atcatttgtt atccattgac tttttggctt gtcgtcctct ctgtcaacaa
     5521 agcggacata atgattgatg atagaaaaag cggtgcgttc agcgtcccga taggtcagta
     5581 aatctacaac cgcataatag gctcgttcgt tcttcatgcg tatttcaaaa cggttcttaa
     5641 tctccgtatc ttcaatttct gtgccattct tgacatactg ctcataattt ttctgataag
     5701 cacacatata taattcgcta cttgttgaac cgagatataa ggtttctcct gtattctttg
     5761 gtaaatcgct accgcatttt tctgtaccgc tgtaatcttt ctgcatacgg aagtaggaca
     5821 tacattcgcc agccttgtat ttttccttta actttggaat atccaatatt cctgctttat
     5881 cattgatagc caaatcaagc cgtttcatca caccaccagc cgtcatacaa tccagcatga
     5941 agtcatacca tgagcgttcc tgtgccagca gataacattc catttgccga cagcctttgc
     6001 ctttcagttc caataaaaca cctaaatgct cctgcatgga acacatgata ttgatgtcgc
     6061 caagtacata tttgctttca tatccgtatt ttccataatc ttcataaagc atgtaattag
     6121 atttcagttg taatacatca cggataatcg ccaatgcgtc cgtagtagga aagcggacac
     6181 gaaagtaatc aatcaataag aatagtggtt cttgcggatt gaaacgctca atctgttttt
     6241 ctatgatttc tcttaattcg tcattcaatg gctgttttcc tttttcaatg cggttgtaat
     6301 attcacggct gataccacag gcaacagcaa gccttgtttg tgttactccg tattcctcac
     6361 gcttttgtct tagttcttca ataaagtttt tatcattcat tcttcaattc ctccaatcaa
     6421 aaaaggcaac taccataatt gataattgcc tgtcattaaa aatctgtata gaaaaagcag
     6481 tcaatcctaa gactaactgc tttgtgtgta tggatataaa attgcttcta atcttgaata
     6541 ggtatccaaa tttcaataac tgaattatca ctattccaat gaaaacgata atcgtatttt
     6601 tcaaagtgta ggataatatc ttttatagga atatacgatg tactaggaat aatttcttca
     6661 taaatcttgc cataagtttc atatatttta tccatagcac caatatgctc cacaaccaaa
     6721 tatttataag acggtatttc tttatacaga aagccctctg gggtaatggt tctttgggga
     6781 atagaacaga aataataaag ttttccattt cttctttcca taaaagcata ttttacccaa
     6841 tttcctgatt gtgaaagata cgcttttttc aaactactgt taaactttct ccaaaactgt
     6901 gtactaatct gaatattctt tttttgataa ttagtcagtt ctacttcctg cccaataact
     6961 gcaaatgcgt ttaattcctt tatagaaaaa tttatcattt taccttgtga gcagtgataa
     7021 cggtataact gtacgcattt accgttataa tacaattatc aatcgttata taccagtttt
     7081 ttccttttct tgtgatttcc gcattagaaa ggctaatttt ttctttgcac cattcaatta
     7141 cattttctac attcaaagat aagttctttt taattctaat aacgccaagt tcagttgtgt
     7201 gaagtcttcc taagttttcc agtaattcat tttccatatt ttgatacctc atttttcagc
     7261 aaatttattt gtatgtttta taccccaatt ataacattct catatcagca ctacaataaa
     7321 aataattaag caccaataat cttattgaac atctccaacc gtaaatcttt cacaccatca
     7381 gtgataaaca ctgagctgac agcaattacg acaactacca taattgcctg cacttaaatt
     7441 tctgcaaaag aaaacgacaa tcaatttctt attttttgaa atcaactgtc tttctcatat
     7501 ttactattca attttcatag tgatataggt acaatttata tcactactgt aaaaatgggg
     7561 ttttgcctta taaatacagc gttttccgct tgttagtgta tagtgtttct tgttttttgt
     7621 gccccccttg ttagataacg ggggctttga acggctcgca agctcgccga accgccgaag
     7681 cgaaccgccc ccttgcaagc atggcggaaa aatcttaagg attttcccgc caacgtggtc
     7741 ggatcgctcc gtcggtttaa cagcttttct gtttctctct agttatctac gcatataaat
     7801 caaatcggtt attccgttat aagtctgtgt tttacaaagc cttaacttta tttccctgtc
     7861 tatgttgccc aaaagacgaa ccccatttcc taacagagta ggaataacag aaatgtaata
     7921 tttatcaatc atatttttgc ttaccaactg ctgaacaaga tttgctccgc cacaaatcca
     7981 aatatccttt cctgcttctt gttttagccg ttccaataat aaaacaggat tttcacttgt
     8041 aaaatgaatt tgttgggaag aattgcactc tttatgtgtt aatacataag ttgtaaattt
     8101 attatatacc cattcttgcg gagaaagttc agtaacaacc tgatgagaag tattccaccc
     8161 cattaagaca gtatctatat ctttggtaaa ttctgtataa gtgtcgatgt tttcattgtc
     8221 atttccatga ccatttagcc aagctactcc accattaccg tccgcaatat atccgtcaag
     8281 gctcatggca ataaataaaa ctacttttct cattttttct cttttataaa tacaaacagg
     8341 aatttatttc tcttttttac tcataaggga acaactgatt gctacaaata aacccattaa
     8401 aatccatgga aatgctgctc ttataaaatc actaaccata ctttattcct ccttaaattc
     8461 caatttcctt agttctacaa actgaaattt ttgcctatga atgtatcttg ttctttatac
     8521 gcaaacaggc ttctgtgtgc ataatccaat gtcttgaaaa cggcatttga attagtccac
     8581 aaatatcttt attacaccaa taatcaatca gccaaattgc tttttcattg tcgtttacta
     8641 cattcaacga ttctaagtat ccttttcttt ctttcggtat ttttcttttc aattcatcat
     8701 aagataaccg ttcaagcatt ttctcagtgc tttcccatac ttcgaaaata tataaatata
     8761 attcttccag attaagctgt tttgaaaagt ctgcaatctg ctgtttcatg agttcatttc
     8821 ctgttgtaat gataggtgaa ttgatacgct cttgataatt gcctgcaaaa aatacttgtt
     8881 catcttcatt tatcactgta tgcacaacta tatcttcaat gcggaaaata tgccaaatag
     8941 aataagcaat cgtcttacta tgataaccat cagcgtttat gaaggggatt gagttaaaat
     9001 cttctctgca taattcttct ttgaaagata ctaaggtttg tattaactga cttcgtaaag
     9061 taagtaaagt atcaataccc ctcttataag tatctttctt ttttatttgt gcctgcattg
     9121 ttttattaag ttcagaccat tccttattca tattcaatcc tctctcaaat tctgatttta
     9181 tcgccccata caaactggaa tttgtcttac aacaatttat atatgcataa aaagcaatat
     9241 atttatgaaa acaccctaaa tccaaaattt actcaattca ttttttcctt acataaaatg
     9301 ttctcgtata tttcacttcc tcaccaagtg tttcccctat caaaacttca aaatcattga
     9361 tatcaaattc tggaaagaat gtatctccat cttcaatatt taaatctact tctgtgatat
     9421 acattttatc tactatttgc aaagcttcct taaatagtcc atatccacca gatatgtata
     9481 catctcgtcc tttagccaat aataatgcat cttctaatga tttaactgaa actaaattat
     9541 ctccttgata ctctgttgtg gtggaaacaa caatattcat tctattaggc aacggatgac
     9601 cgatttcttc ataagacttt cgccccataa taaccacatt acccgttgtt aactctctaa
     9661 attgcttttg ttctcccttt attttccatg gtatattacc attcttgcct ataacattat
     9721 tctttgacct cgcaacaatc aaacctatca tctatttcat cctccataat atgatgtcgt
     9781 acctgtgatt ccatcaagtg caccacatct taacaaactc aaaattatct cgaactaacc
     9841 agctcgacaa attccttttt tgtcttcatc tgcattatat catattgtta taagcattgc
     9901 catttctttt ctgcttttga aaagttactt atcagcctat gttttgtaaa gggtgatatt
     9961 cagcatttct cacgcaacaa gcccttgaca aaccatagac taataagttt taaggtaatc
    10021 aagcagaaat gaaatagtct gctgtttcac ggcaacaaaa aagagatacc aacacactta
    10081 tgtagtatct cttagtctag tagcaatatt cttttgtttg caagcaggta aacatgggct
    10141 gtttcttaat atctttttgt ttgggaaaca cctttttccc atcctttcac cgttgccatc
    10201 tctgctgcgc acaatgccac agggataaac catagcaaac cgcctaataa caagaaaaat
    10261 actaagctga agcctgaggt cgcgaatgtg ggatattcat ataatgaaac gaccattgag
    10321 atggtcatag ccaaaaagcc aaagaacgtt aatttttttg caggaatttt cgttttgctt
    10381 tgttgcatga atcttcctct tttctatttg aatttgtttc aattgagttt gttgccaaag
    10441 aagaaaaagt ccaatccttc ttcttgttct tcttttgtag tagtgatcgt tgtaaagtgc
    10501 atcgccgatt ttaaaatcac tgttgtcagc acataggcat acacggcagt aatgatgaca
    10561 ccaatcactt gaatccctaa gaagtaccag ctgcttggat tcgttagtaa atggctgtta
    10621 gatgcaaaga tccctatcag aatcgtacct gtgaatccgc ccatgccatg aacaccccac
    10681 acatcaagtg catcatccca ttgtttcttt ttacgaaaat caacagcagc atgacagacg
    10741 atacccgcaa tgatcccaat cagcatggca ctttttgctt ctacataacc ggcacaaggc
    10801 gtaatggctg ccaatccagc aacggatcct gttagtacgt cgacaaaatc aaagccattt
    10861 ccttttactt ttgcaatgat cagccaagta atcatcgcaa atgcaagagc gatgaatgtt
    10921 gaaccaaatg cattgactgc ttgttcatct gctcgaagtg caccaccgga attaaaaccg
    10981 aaccagccaa accaaagcag tcctgtaccg atagcagctg ccataagact gctatgctga
    11041 cttctttctc ctaaaggaat ctttctttga ccaagtgtga ggatacatgc taaagagccg
    11101 aatccagctg tcgtgtggat gactgctcca ccagcgaaat ctctaaatcc catctgatcc
    11161 aaaaatccgc caccccatat ccagtggcaa acagggaaat aaatcagcag attccaaaaa
    11221 ataacaagta taatatagcc ttttaaattc aatctccctg caaaagctcc tgtcatcaaa
    11281 ggaacggtaa tcacgataaa catcagttgg taaagaaaga acattaaaaa tggaatcgtt
    11341 gcaccatgaa acatgttggg gaagcttgta acatgtctca tcaaaaagaa atctgccggg
    11401 tttccaataa caccgccgat atcccgtcca aaggccaatc cgaatccacc aaatacccac
    11461 ataatcgttg ttatcccaac ggagagaaaa gattgagaca tgatcgttag tatattattc
    11521 ttccgtgcta atccgccgta aaaaaaagct agaccaggag tcatcaggca aaccattgct
    11581 gtacataaaa tcatgaatgc tgtatctcct gtattgatag gtaatgtatt catttttttc
    11641 gcctcctttc tacgattacg agtataaaag aaggtgtagt tgtttttttc ttctgttttt
    11701 ttgaattaat acttgcattt ataaaagaca gacaattaat aaaagcttga tgtgttgtgc
    11761 tctttttcgt ttttcacaca aataaagaca aaaaataaac ggtcttcttt ttatagaaaa
    11821 ccgtttattt tcgtcttttt ttatttttaa agatattttt tcataaggaa taaagaatac
    11881 ttcatataaa atatataaaa aagaaccgca gcgagtgtcg ctacggttct caaatttgtt
    11941 cttcatttca acataagttt ttcttcaatt tgcttataat ttacgtgcaa agaatgaaac
    12001 gactaatact aaaatgattg caccgatcaa tgatggaatg atagccattc ctgcaattgt
    12061 tggtccccaa gttcctagta aagcttgtcc aattgctgaa cctactaaac cagcaatgat
    12121 gtttgcgatg atccccattg atgatccttt attcgtgatt gctcctgcaa ttgctccaat
    12181 gataccgccg acgattaatg cccaaataaa tcccatttta aattcctcct aactgacttc
    12241 ttacttctta ttgtacacgt ggttcgttat tttctttgac tttttgtgca cctttgccta
    12301 cagcagattt tgctttatct gtttggcgtg aagcaaattg tcctgtggat tttgctgctt
    12361 ctgttacttt gtcttgtact gtttcttgat ctttttggta ttcttcttgt gttttgatgt
    12421 ctgcaacatt cacattcact tcgataacat ctaaatgtgt catattgttt acttcagtac
    12481 tgatcaattc tttgatttgg ttgtaaatat cttcgatgtc tttaccgtat tctacgatca
    12541 catctagatc cacagctact tgtttcttac ctacttctgt attgatacct gctgttacgt
    12601 tatctgtgtt gactaatttt tcagccatat ttgagaaaaa tccgccatct aatgtcaata
    12661 gaccatctac atctttcaat gcataaccga tgattttttg gatgactttg tcttcataag
    12721 tcaattcacc attcatcatg cggttttctc ctttttcatt tgccaaaact gatccatttg
    12781 ttgtattcat gtttttgttt tccatctttg tttcctccta tgatttaaaa aattgatcaa
    12841 ctattcctga ctgtttcaaa taaaaaccga ttcctgcgcc aattgctgtc atgattatga
    12901 tcaacaatgt cttgaaaaat ccaatcgtta accataaaac agctagcagt agtccaagac
    12961 ccccaaagat aatagggagc ttgtatgctg aaaatagttc tttcattatt tggccctcct
    13021 attccactcg agagtgcttg tagtctcttg tatcttcttg ttcttcataa ccgctgtatg
    13081 tgactgccac tttgaccgtt tcttttgttc cgagtacttt agttacttct tcttcgatat
    13141 cttgcatcaa tacacctgtt ttccctacta atgaagaagt tcgtttcaag tctccgttga
    13201 catggatatg gattcgattt ttcgttgaac gtatccgtac tttaggtgaa tgaatgaact
    13261 cttcttcgtg taaatgggaa cgtaccaacc cttcgatcgc ttttttgtcg attgttagtt
    13321 tgccatcttc ccgtttcata acgaaagttc ctcttgactt tggatagaaa agaacgacta
    13381 gcaagatgat aatagcaaga acggcaaata ctacagctgc ccagaaaata tattgttgca
    13441 tgtagtaatt cgttaaagta aagaagcgaa atcgttccaa ttgttcaggc ataatatacg
    13501 gcgcattgat caacgcaatc gctagaaata ctgaaagtag cagtaaactc atgataacac
    13561 cgattatttt taacccctta ttcattttcc acctcctata aacttatttt taccctgtaa
    13621 ataactctac cgacaatcac agtatagata gcgaacagtt agcatagcat cagtaaataa
    13681 agcgctacca aacattgctg gatcataacg gcgttttatt gccagtctga tactgaaata
    13741 acaattgctt acttattctc tatttacttt tgattgctgg ctatacttaa aggatattat
    13801 tcaagattaa aaattgcaaa gattatgctc agttattatt acaagattct atttatttat
    13861 taaatttttt cggagaatta aaaaatacaa taaataactt tagttacttt aatgcgttct
    13921 tactttattt ttatattatc atttttctta ttcacttctt tggtttatac tattcttata
    13981 aataaaaacg aggtatgtcc tatgatgaaa agattgtact attcattgat cataacgatt
    14041 ggctatttga ttgtatcaaa tctaggaaat atggtatttg gtatcagtaa agaattcagt
    14101 tggactacta cattatggga atctctattc tttttcatct tcgtattttt gttacaaaat
    14161 tatcgaaaaa aataaacatg tgccatctat ctgttggcac atgttttagg atttatggta
    14221 ctttttattc ggcaatccat tttatttttc tacttcttct taatacctct aacacttttg
    14281 atttataaac taatccaatg catctacttg cgcttattaa gctatttcag ctagatagta
    14341 ccaccccatg cgtcaatgct tgattttcct tttgtataac attgtataag caataaagga
    14401 caaataagtg gtgattacac caaaccattg aataagaaaa attctgatac gtaaataaac
    14461 atgaacttga aaaaattaat gaatcggtta gctactacgt agttgtcttt tttctcatac
    14521 agttctgtta acttacactt ctcttcgcta gataataaaa aatcctccaa ctgtattgtc
    14581 caatataatc ccaggcatct aaactagcat tatagaagag acacacatag aatctaaaat
    14641 aactggtttt aaatgtttct tacaatcaaa aaaagctgtt aaatgcactc cacaccttca
    14701 tactctatca ttttttataa acgatatatt tttattgttt ttcgataaaa aatagttgtt
    14761 ttacaataat tattctgcta tattttagct atcaaagata aaagagggat aaaaatgaaa
    14821 tttaaaacat tgctattaaa atttgtcgtt ttaggtataa gtgccgtatt tattttgttc
    14881 ggcgtacttt tttatactca gatcgctaca agcgaaaaag gattcaattt tgattggcag
    14941 accacattat ttgtttctgt catttacttt gcccttctcc tagggatcgt tattgcttat
    15001 ttcttattga aactattgaa aaaaatcgaa gataaccaag ctttttccac tcaaagctta
    15061 aacatactaa aaaaaatcga acgaaccatt ttgtttattg gtttcgcctt ttttggctta
    15121 cttcctaaat ttatgaaagt agcggattta gacgatgctc caggtttgat gttggtagga
    15181 atcgctgtcg tattcattcc ttttgcagta tacacattaa caagcgtgct ggaaaaactg
    15241 cttgaacatg cgattttact taagacagat catgatttga cagtgtgagg tgcgacatgg
    15301 caattatcgt taatttagat gtagtattag ctcaaaaaaa aatgagtgtg actgaattga
    15361 gtgagaaagt gggcattacg atggcaaaca tctctattct aaaaaatgga aaagcaaaag
    15421 ccatccgttt ttccactctc gaaaaaatct gccaagtttt ggagtgtcaa ccaggtgatt
    15481 tgttgaccta taaaaatgac tcgaatacta taccagaaga ataaaataaa gtagaacaaa
    15541 cagtaaataa aaaataatct gaatgataga tgtgtgagac ttttagaagt tatttacgtc
    15601 tattattcag attttttatt tcgattgaat accgttttat ctataaataa gatatgtact
    15661 gactcataca aagggggcct tttcttctcc atgtactatc acttttcctg atacaactgg
    15721 tttcctgtta cgttgtagtt ggatctgctt ttagtataat actttattca ttccaccaga
    15781 ctgatagcag tttgagcagt ctaaataatc atgtagtatt acaggccatt ctgtttcata
    15841 ccttgaagtg cttaaaactt cagctaatgg cccattgtgt taactcttac tccattcttt
    15901 tttagtaagc tcccatatat actgtaaagc cttctatata ttatgtcatg ttgcttttta
    15961 acatttacta tgtagttttg ataaaatgat ttgaataaca gtgatggaca attctttttt
    16021 atttttaaca ttattttgat aataatg
//
LOCUS       DACEDM010000071        15825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEDM010000071 DACEDM010000000
VERSION     DACEDM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.071"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(58..360)
                     /locus_tag="IWO50_002226"
     CDS             complement(58..360)
                     /locus_tag="IWO50_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359329.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(580..946)
                     /gene="ssrA"
                     /locus_tag="IWO50_002227"
     tmRNA           complement(580..946)
                     /gene="ssrA"
                     /locus_tag="IWO50_002227"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1117..1890)
                     /locus_tag="IWO50_002228"
     CDS             complement(1117..1890)
                     /locus_tag="IWO50_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0359330.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2034..2384
                     /locus_tag="IWO50_002229"
     CDS             2034..2384
                     /locus_tag="IWO50_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0359331.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2365..3081
                     /locus_tag="IWO50_002230"
     CDS             2365..3081
                     /locus_tag="IWO50_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0359332.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3081..4529
                     /locus_tag="IWO50_002231"
     CDS             3081..4529
                     /locus_tag="IWO50_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0359333.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4599..5108)
                     /locus_tag="IWO50_002232"
     CDS             complement(4599..5108)
                     /locus_tag="IWO50_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359334.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5098..5823)
                     /locus_tag="IWO50_002233"
     CDS             complement(5098..5823)
                     /locus_tag="IWO50_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359335.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6046..8166)
                     /locus_tag="IWO50_002234"
     CDS             complement(6046..8166)
                     /locus_tag="IWO50_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0359336.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8396..9574
                     /locus_tag="IWO50_002235"
     CDS             8396..9574
                     /locus_tag="IWO50_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0359337.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9590..10348
                     /gene="aroD"
                     /locus_tag="IWO50_002236"
     CDS             9590..10348
                     /gene="aroD"
                     /locus_tag="IWO50_002236"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0359338.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10371..10856
                     /gene="ybaK"
                     /locus_tag="IWO50_002237"
     CDS             10371..10856
                     /gene="ybaK"
                     /locus_tag="IWO50_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0359339.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10927..12180)
                     /locus_tag="IWO50_002238"
     CDS             complement(10927..12180)
                     /locus_tag="IWO50_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0359340.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVYLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12297..13646
                     /gene="gdhA"
                     /locus_tag="IWO50_002239"
     CDS             12297..13646
                     /gene="gdhA"
                     /locus_tag="IWO50_002239"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0359341.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13758..15104
                     /locus_tag="IWO50_002240"
     CDS             13758..15104
                     /locus_tag="IWO50_002240"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0359342.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15412..15798)
                     /locus_tag="IWO50_002241"
     CDS             complement(15412..15798)
                     /locus_tag="IWO50_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0359343.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5174 a         2830 c         3179 g         4642 t
ORIGIN      
        1 cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta
       61 atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc
      121 gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat
      181 tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa
      241 taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt
      301 gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat
      361 aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat
      421 tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg
      481 ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa
      541 taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg gcgggagtcg
      601 aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac tcatttaagg
      661 attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga tgttctcttt
      721 ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc ttactcgagc
      781 acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa gcgtaagagt
      841 tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg taacctacga
      901 aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta gctaacaagg
      961 gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat tcaataaaaa
     1021 agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa aaataagcga
     1081 ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa aatacttcaa
     1141 tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa gttcttttaa
     1201 tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca aggcaaaatc
     1261 attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt ctttttgcag
     1321 acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg agtaaggacc
     1381 attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac ctgattgaga
     1441 taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa tttccggaac
     1501 atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg tacaccagat
     1561 tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat catacatttt
     1621 ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta ctcgaatgaa
     1681 agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag agtggatttc
     1741 tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta atgcgttcat
     1801 agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc catcaagatc
     1861 aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat tggaataagt
     1921 ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct gaacatattg
     1981 gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa agaatgattt
     2041 ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt ctgccctcaa
     2101 aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca gacgctcatt
     2161 ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta ctgatggcat
     2221 tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt gaaatgcttc
     2281 tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta cagaaatatg
     2341 acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa tcaatctgtt
     2401 tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat
     2461 ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta
     2521 gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca
     2581 agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc
     2641 ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct
     2701 cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt
     2761 tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa
     2821 gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt
     2881 actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg
     2941 gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa
     3001 aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc
     3061 gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa
     3121 tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga
     3181 ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc
     3241 aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc
     3301 tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg
     3361 aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat
     3421 ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac
     3481 aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag
     3541 cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga
     3601 acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg
     3661 cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg
     3721 gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg
     3781 atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa
     3841 atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa
     3901 tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg
     3961 cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg
     4021 attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag
     4081 aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat
     4141 tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa
     4201 tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta
     4261 ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt
     4321 ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa
     4381 atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc
     4441 ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt
     4501 tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga
     4561 tcttctatac cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga
     4621 tcaatataag gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt
     4681 tcttcatgag ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt
     4741 tcatcaaatg tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat
     4801 tgcagcttta ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga
     4861 gagatatcaa gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca
     4921 tccaacagga catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg
     4981 atcgcaaaat tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta
     5041 tgctgttttt cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca
     5101 ggtttcatta cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa
     5161 tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag
     5221 tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat
     5281 cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt
     5341 ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac
     5401 gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat
     5461 aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt
     5521 gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa
     5581 actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt
     5641 agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg
     5701 caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca
     5761 atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc ggattatttt
     5821 catacttttc acctctataa aaagtataga aaatgaatat gaataaagta tgaaattcct
     5881 ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag gttgtgaaga
     5941 agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg
     6001 agcagcgaca ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact
     6061 tgtggtcgtt ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg
     6121 tccgttgtgt tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt
     6181 taatttctct aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact
     6241 attcgtatag aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt
     6301 atcaactttt tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat
     6361 agcttcacca gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac
     6421 aaaatcacca tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc
     6481 taactgaata taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc
     6541 aacttgtccg ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat
     6601 ataagggaca cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc
     6661 aactaattca gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag
     6721 gacgatgact gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac
     6781 ggcggtatct aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt
     6841 ggctaacggg aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac
     6901 agcaatgaat tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg
     6961 gaaaaatggt ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata
     7021 agaagaatca aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa
     7081 tgtaccagcg ttcccatgga ataccgcact aatgtaacct tgtgtttgac ttaaaatatc
     7141 aggtgcagct tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa
     7201 gccaaacaat gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg
     7261 atggaaaaag ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg
     7321 tgtaacttca tgctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg
     7381 gaagctttct aaatggatat aaatcacatt ttttcctcta gcgagcccga acaaatcgtc
     7441 atttggtgct gcataatgtt cttttacata attttctact tctttcatat cattgggact
     7501 tgcttctgcc cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc
     7561 attcaagcca agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg
     7621 acgatcagct tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat
     7681 cgcaaaagcc attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag
     7741 cagaacagtc aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg
     7801 aatggcactt tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt
     7861 aatgaagtcc gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag
     7921 aaaataaatg accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat
     7981 tccaagtaat aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc
     8041 actttcgatt ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa
     8101 aacgactagt aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca
     8161 tttcaagata atcttccttt ctgcacttct taatcacaga agtgtcctga aaccatgtta
     8221 cacagctcac ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca
     8281 gaagtttatg ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga
     8341 caagtcctat gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa
     8401 agtaaaagta aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa
     8461 agaagacctt gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca
     8521 aaaccaattt atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt
     8581 gagccagcag aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa
     8641 agaaaaaaga actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg
     8701 agaaggagac gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc
     8761 ttggtataac gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat
     8821 ctacccggaa atcataggag gctatgagaa aattcgattt gaatcagtaa atctgcctga
     8881 gagccagttc atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt
     8941 aaaatttgcc gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga
     9001 agtacgggga tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag
     9061 ttataccggc gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt
     9121 cgatctagca aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga
     9181 tgtcgatcag caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag
     9241 aaaacaattt ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa
     9301 aaaagtcttt cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt
     9361 agcagaagac gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt
     9421 ccaaacaatg attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt
     9481 atatcgtctg ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa
     9541 agtgtttgtt tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggttcgggt
     9601 aaagggattg aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt
     9661 ccgagaggaa gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga
     9721 atggcgagtc gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt
     9781 tcttatccat gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga
     9841 aggcggagaa caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca
     9901 cacaggatta gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag
     9961 actgatagag gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa
    10021 agaaacacca gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc
    10081 cgatatcgga aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga
    10141 gttgactaga cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa
    10201 tcttggtaaa atttctcgtc tggttggcgg aacgacgggt tccgttatga catttggttc
    10261 cttaagcagc gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat
    10321 tattcaatta ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga
    10381 aaaaaatagt aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag
    10441 agcacgaata tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc
    10501 cagaaagcca atctcgtata tttaaaacac tggttgctgt gggcaatgtg acggggcctc
    10561 ttgttgctgt gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag
    10621 ggaataaaaa agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac
    10681 gtggtggctg ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg
    10741 cagaaagcta tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag
    10801 cacctcaaga tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca
    10861 tacgtataaa agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt
    10921 tatacattat gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc
    10981 tgtgatatta aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag
    11041 accatcaatg attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc
    11101 gccagttaca aatgatttct caataggaaa agttttgttg attgatagct taaactcttt
    11161 gcatagttga acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc
    11221 catttcaagc cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc
    11281 attgaccaag ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg
    11341 tttggtttgt gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa
    11401 ttctttattg acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt
    11461 gaaaagtaaa tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa
    11521 agtgatgtct tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga
    11581 tatcaagggg gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat
    11641 cttgtaccca tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat
    11701 aatggcagga gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt
    11761 gtggatcaat ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg
    11821 attgagcaat gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg
    11881 atcctcttct tttaaatata cgccttgtga ttcaaagaag ttcatgatat cgaaattatt
    11941 ccattgcgca aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc
    12001 atcaacagga cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt
    12061 tcctgctttt ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat
    12121 cgctgccatc atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat
    12181 tccctcacct caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg
    12241 agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga
    12301 caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc
    12361 aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca
    12421 ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta
    12481 ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc
    12541 gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg
    12601 tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg
    12661 gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata
    12721 atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat
    12781 cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg
    12841 gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt
    12901 tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac
    12961 atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg
    13021 ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat
    13081 gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag
    13141 aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta
    13201 tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat
    13261 gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca
    13321 aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg
    13381 aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg
    13441 ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc
    13501 agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg
    13561 gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag
    13621 caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt
    13681 ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc
    13741 tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg
    13801 tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac
    13861 gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg
    13921 acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag
    13981 tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct
    14041 tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct
    14101 ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc
    14161 gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt
    14221 tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta
    14281 tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt
    14341 gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg
    14401 gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg
    14461 atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag
    14521 caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac
    14581 caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag
    14641 accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac
    14701 aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc
    14761 gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag
    14821 gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag
    14881 acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt
    14941 atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc
    15001 cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac
    15061 caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc
    15121 tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt
    15181 taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt
    15241 cacagtccct tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga
    15301 ctttaagaac agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca
    15361 gtaagcaaaa aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag
    15421 gcctaatttt tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat
    15481 gatccgcttt tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt
    15541 ggcagagtca ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc
    15601 ataagcatct acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat
    15661 atcagtttct gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt
    15721 agactgatag tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa
    15781 ccctaaaatt tttttcatga aagatctcct tttttatttt tatta
//
LOCUS       DACEDM010000072        15669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEDM010000072 DACEDM010000000
VERSION     DACEDM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.072"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWO50_002242"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWO50_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0359344.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWO50_002243"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWO50_002243"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0359345.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWO50_002244"
     CDS             complement(2169..2906)
                     /locus_tag="IWO50_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0359346.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWO50_002245"
     CDS             complement(2997..3524)
                     /locus_tag="IWO50_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0359347.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWO50_002246"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWO50_002246"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0359348.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWO50_002247"
     CDS             complement(4401..4886)
                     /locus_tag="IWO50_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0359349.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWO50_002248"
     CDS             complement(5039..5236)
                     /locus_tag="IWO50_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359350.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWO50_002249"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWO50_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0359351.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWO50_002250"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWO50_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0359352.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWO50_002251"
     CDS             9211..9423
                     /locus_tag="IWO50_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0359353.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWO50_002252"
     CDS             9425..11104
                     /locus_tag="IWO50_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0359354.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IWO50_002253"
     CDS             complement(11358..11558)
                     /locus_tag="IWO50_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0359355.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IWO50_002254"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IWO50_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IWO50_002255"
     tRNA            complement(12249..12322)
                     /locus_tag="IWO50_002255"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="IWO50_002256"
     CDS             complement(12634..13032)
                     /locus_tag="IWO50_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359356.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="IWO50_002257"
     CDS             complement(13025..13477)
                     /locus_tag="IWO50_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359357.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13871)
                     /locus_tag="IWO50_002258"
     CDS             complement(13464..13871)
                     /locus_tag="IWO50_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359358.1"
                     /translation="MSFLLLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENCWIGEEKLNEIY"
     gene            13850..14152
                     /locus_tag="IWO50_002259"
     CDS             13850..14152
                     /locus_tag="IWO50_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359359.1"
                     /translation="MLIEGKTQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(14188..14559)
                     /locus_tag="IWO50_002260"
     CDS             complement(14188..14559)
                     /locus_tag="IWO50_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359360.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(14561..14962)
                     /locus_tag="IWO50_002261"
     CDS             complement(14561..14962)
                     /locus_tag="IWO50_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359361.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(14976..15383)
                     /locus_tag="IWO50_002262"
     CDS             complement(14976..15383)
                     /locus_tag="IWO50_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359362.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT         4671 a         3227 c         2573 g         5198 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaacta agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 caattctctt ccccattgca tactttctta catatatcat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaataga
    13861 aggaaagaca caattatggt ttaaatttga tccttcgaat agatttatca aagattttta
    13921 taaggtgtgg gattcagaag ttttctttct agcaatcgaa gatagcttat taatcaatct
    13981 ctactattct aataagaact actttaaaat ccctgctgca aaaactagaa tgaagaaaga
    14041 tgtatacttt ttgtttgata tcgtgactga cgtgccggac gcacgtagcg atcatcggcg
    14101 ttatgactat ataaagtata ctttcgttga tccagaaagg tataaagatt aaataaaaaa
    14161 ataacaaaca aactcgcttg aaattcatta ggtgagtttg tttattattt tttctgcttc
    14221 tcgattcatc agcgccatat ctgttttcgt aatggtttca ttaggaatat agtcagcaca
    14281 ttttcttttc tctaacattc tatacccact attatgtatt cctaaggtat tcaattcctt
    14341 aactgttgaa aacaaattcc aaacggtttg gtggcttcct tttccgctac ttccattata
    14401 aataatatca taattttctt tcaaatactc atcagatttt ttaaaagcac tgtagtatgc
    14461 tcttgaaaca gcagttcttc tataagattc tttagaatta tatttatcat ctgattcaat
    14521 ttgtttggaa atagataaat attcattaaa gtcaaacatt ttattccccc aaagttgcta
    14581 ctacagattt ccccatatat ttatagtcag ataatacatt atcgagagta tccaaaacat
    14641 cgtaatctga ggaaaacaat tcagatgttg ttttgataac aaagaaatac aagtttaaat
    14701 cttcatcata ttttatattg atttcagctt ttctttttaa aaccgtattt atcacatcaa
    14761 aaattttgcc atcattactt gaaattgtgt taatctcatt ttttgtagta attacaagtt
    14821 gattatcatt tttagttttc ggagtagaac ttgcatccac aacaactgga gatgctagaa
    14881 ttaccgatgc gcaagcaaaa atcgcagcaa aatttattaa gctaccactc cgatgtgtat
    14941 ttgatggtac tatatcaagc atccgtatcg tctccttaat ttttttccac tggtttgttg
    15001 accttgatat caaagatttg ccttccaatc tcagttccat taaatttaac aacagcataa
    15061 tgctctcctg cttcttcaac caagacattt ctggcgttta tattaaagtt tatatcgtta
    15121 acttgaggag gcaagcttac cgatacttca cccgtagaaa aaatttgttt ttcattcttg
    15181 tttaaaactt caatttctac tatttttatc ttttctgtat cctctatctc agacaccaca
    15241 gctgttacag aaaatgataa agcagtagga attaaaggcg cattaataga aagagctgga
    15301 ttaattactt tattaataac attagtgcca aattgctgac tttctacatc ttctgaaatt
    15361 attaattgta ctctataaga cattgatttc tcctaatttt tacatttatt taataaattt
    15421 atgtttcact tttgctagtc aatttagttt aacaatagta acaaagcttt gtcaacggaa
    15481 atattagcta attttgtaag catatttata agtatatttg taagcatatc tgacatttaa
    15541 tcagtagtaa aagtgcctct atataaggaa caaactgttt ttttactatc taaattcgta
    15601 tacagtttta ctaacaaatt agctaacaat attagcaact atatcaacaa ctatatgtac
    15661 ttcccctca
//
LOCUS       DACEDM010000073        14374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEDM010000073 DACEDM010000000
VERSION     DACEDM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.073"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..590)
                     /locus_tag="IWO50_002263"
     CDS             complement(<1..590)
                     /locus_tag="IWO50_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0359363.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKV"
     gene            complement(896..1843)
                     /locus_tag="IWO50_002264"
     CDS             complement(896..1843)
                     /locus_tag="IWO50_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0359364.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            2033..2302
                     /gene="rpsN"
                     /locus_tag="IWO50_002265"
     CDS             2033..2302
                     /gene="rpsN"
                     /locus_tag="IWO50_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0359365.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(2478..2840)
                     /locus_tag="IWO50_002266"
     CDS             complement(2478..2840)
                     /locus_tag="IWO50_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0359366.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2851..3972)
                     /locus_tag="IWO50_002267"
     CDS             complement(2851..3972)
                     /locus_tag="IWO50_002267"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0359367.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3988..4338)
                     /locus_tag="IWO50_002268"
     CDS             complement(3988..4338)
                     /locus_tag="IWO50_002268"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0359368.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(4468..5979)
                     /locus_tag="IWO50_002269"
     CDS             complement(4468..5979)
                     /locus_tag="IWO50_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0359369.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(6301..7653)
                     /locus_tag="IWO50_002270"
     CDS             complement(6301..7653)
                     /locus_tag="IWO50_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0359370.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7806..8882)
                     /locus_tag="IWO50_002271"
     CDS             complement(7806..8882)
                     /locus_tag="IWO50_002271"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0359371.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            9078..10823
                     /locus_tag="IWO50_002272"
     CDS             9078..10823
                     /locus_tag="IWO50_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0359372.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(11103..11540)
                     /locus_tag="IWO50_002273"
     CDS             complement(11103..11540)
                     /locus_tag="IWO50_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0359373.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(11524..12897)
                     /locus_tag="IWO50_002274"
     CDS             complement(11524..12897)
                     /locus_tag="IWO50_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359374.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(13219..13707)
                     /locus_tag="IWO50_002275"
     CDS             complement(13219..13707)
                     /locus_tag="IWO50_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0359375.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(13729..14331)
                     /locus_tag="IWO50_002276"
     CDS             complement(13729..14331)
                     /locus_tag="IWO50_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0359376.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4103 a         3099 c         2591 g         4581 t
ORIGIN      
        1 actttattga tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc
       61 aataaagtcg aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt
      121 gctcctttat cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg
      181 atttttggtt tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt
      241 ggcaagtaaa tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt
      301 gaatcaacga attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc
      361 ccaatggcca cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt
      421 ggcagctctc cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat
      481 agcgaaggga tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc
      541 cgtttccagt caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc
      601 cttcttcttt tttcagctga attactttac catgtatttc atactttttt caatcggctt
      661 ttttcaaaaa agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac
      721 atattgagaa ataattaagg attacagcct ttactttatc atgaaataaa agggaaagat
      781 aattttatgc caaagttttt atctattata cgtatacgca aaaaagctaa aacatttttt
      841 gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt atttcttatt
      901 tttcgaatga caggatgatt ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt
      961 ggatgacatg cttaagtgaa gcagacagat tcattgaaat aaaaacatga aaggctcctt
     1021 tttggtaggc tttgattatg ccagcaaagg cttctgcatc tagatattcc tttcttggaa
     1081 caaacgattc ataaaagcct tccttttttt gccgcaaatc tagatctggg aggtcattta
     1141 atgcaacata cctgccgcct ggtttcatga tctggatgcc ggtttctcct ttgatacttc
     1201 ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa ttgcagacct ggatttgttt
     1261 tatcataagc agcaaatgct gaagctccta atttttttac cttttcctca tttttactgg
     1321 atgcactggt taagattcgg attccctttt catggagaag ctggatcaga cttgacccga
     1381 ccgcacctga tgctcctagt atcattacag tatctgtcgg ctggatttcc gtcaaatggt
     1441 tgattagatt ataagccgta attcctggcg tgaccattcc ggctgcttct tcccaactca
     1501 ttttatctgg aatttttgct acttttgctg atggcaacac gacttcttct ccgtaagtcc
     1561 cgccgactgc atggacagct actcgatcac ctacatgaac agttgtgaca tctgaaccaa
     1621 tctctgtcac aataccagcg ccatcattac ctggtacata agggaatttc aatgttcgta
     1681 tctctttcat ttctcctaaa cgcaaggcaa catcataagg attcacactg aatgctttta
     1741 tttctacacg aacatgtcca gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa
     1801 catcttgtgc ttctccgtaa ccgttaattg caaatcttcg catggtcacc atctcccttt
     1861 ttctttaagt ttacgtttat gcctttattt tgacaaaaaa aatgtctttt cgttttggcg
     1921 catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg tgttaaatta tttatctaaa
     1981 agcgtaaaga ttacgattta cgctaataaa aaataattgg aggaatttgt tgatggctaa
     2041 aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg attgaacgct atgctgcttt
     2101 acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg cgtaaacttc ctttagatgc
     2161 tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga cgccctcgcg gatacatgcg
     2221 taaattcggg atgtcccgta taaaattccg tgaattagca cataaaggtt tgattccagg
     2281 agtaaaaaaa gccagctggt aatccaagtc tggctgtaat gtaaaggagg agggcgcatg
     2341 gaccactcct tggatcatca aaaaagatac gttcgcttct tctagcagaa atgctggtac
     2401 tagaagcaga cgtatctttt tttacgcaca gctggtcctt ttttatatgt aaaggctgca
     2461 tggttacttc gctatattta tttagtaaca ggagtcagtg cttcgatacc cacactaatt
     2521 ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca aatggcagac cttttctttc
     2581 aatcttgatt tatcgatcgt tctgatctgc tccaacagga taacggagtc tctttctatt
     2641 cctgttccag tagctttgat cccaatgtga gtcggaagct taggtttagc catttttgcc
     2701 gttatggccg caacaatgat cgtcgggcta aaatggttac ctagattatt ttgaataacc
     2761 aaaactgggc gtgttccgcc ttgctccgat cccacgactg gagaaagatc agcaaaataa
     2821 atatctcctc tttttaccat aggatcctcc ttattcttga tattctcttg gtatccgctg
     2881 tcctaatccg caagccactt catagtggat cgtacctaat tgatctgcta tatcctgcat
     2941 agtgatttct ttgtctgcat ttttgccgat aagcgtcact tttgttccga caggaaattc
     3001 ttgaggcaat cggatcatca gttgatccat acatacacgc ccaatcgttt cacagtagtt
     3061 cccttctacc aatagagaaa aaccctgcat ctttcgtggc cagccatctg cataaccgat
     3121 cggtatcgtt ccgatccact ctgcttcagg agtgatatat gtttctccat agccgattcc
     3181 ttcacctgct gggagttttt tgacttgaat caattcagaa accagctcaa gtgctggctg
     3241 caatggataa acttccggta gtgcgtgccc agagggatta agtccgtaca ttgctactcc
     3301 ataacgaatc atattgccga ttgtttcatc atgccataaa gccgttgcac tattgctgac
     3361 atgtacatat cgaggaagtt cgggaagttt tttcagcact tctataaagc gttctttttg
     3421 cagattccaa tatgtatcat ctgcctgatc tgctgtagaa aaatgggtaa ataaaccttc
     3481 ccattctaat acacgacttt ctttgatgaa tgcagctgcc tcttttactt cttcaggtgt
     3541 acaaaatccg atacgaccca tcccagtgtc tgcttttaga tgaatcgaaa gaggcgtctc
     3601 tagcttactt ttctctaaaa cagcagctgc tgcttgcaac cattcccgtg tacctgcagt
     3661 tactgataaa tcatgagcaa cagctacagt cagtgcgtcg taaggaacca tattcaagat
     3721 aaggatgggc tgagtaaatc cagcttctct caactcgact ccttcatcta aatttgaaac
     3781 acaaaagccc gaagctccac ctgcaactgc tgcctcagct gtttcaatag ctccatgacc
     3841 gtatccgttc gcttttacta ctgcaaacaa ttcttttcct tgcggaagtc ttgcaacttc
     3901 atttgctaca ttttcagtta tagctttttt atgtatcact gctttcgttg ggcggtgcca
     3961 agcgacaacc atcgtttttc cttctttcta gtcttccaaa ataatttgtg caaaagctgt
     4021 ttcatttgta tgagtaatac tgacccatac attgccttca tgaggtgaac gcgtcacaac
     4081 aggctgacca ttttcatttt ttaaaatctc gacatcttga aaggtaactt ttccgatccc
     4141 agttccccaa gctttagaga aagcctcttt gcaagcgtaa cgcccgccta aaaattctac
     4201 ttggcgatga aaaggcaagg actggaatag tttcatttca tcagaggtca agattcgtgc
     4261 aatgaatttt ggtttttctt cgatcagccg agtgatccta ggaagttcga ctgcatcgat
     4321 cccaattcct tttatcatat gcttttctct tttcaataaa tttagaacac tctcattcta
     4381 ccaaattata accattttgc cacaaataag tccacaaata acaaaaatga aagtttctct
     4441 taaaaaagag aaactttcat tttatgttta atcgttattt gaacggatta caaatccgcg
     4501 ttttttatca ttgtgacgtt taccgccatc tttttggctg ttattatttt tgctataacc
     4561 gccttttgat ttgtttccac gataattacc gccgtcacga cgattacggt tgtttccgcc
     4621 accacgtttg ccatttttat tgtatccttt tttctgcatt ggcaatgggc gttctggtgt
     4681 gatcttcact ggcactgcat cagctggatc ttttgctgtc gtttttagca aaagtgcaac
     4741 taaatcttgt gcagaatatt cttctaataa tttatctgct gtttgcaggt atttatctag
     4801 accattttct gcaagttttg tttcgatttg ttcaactgca gcacctaatt gacctttgaa
     4861 tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt tttttcgtca agttttcaat
     4921 cacatgcaag tagtccattt catttggtgt aacgaaagtc acagacatac cgccttttcc
     4981 ggcacggcct gtacgtccga tacggtgaac gtagctttct ggatcttgag ggatatcata
     5041 gttgtacaca tgagtgacac ctgaaatatc taatccacgt gcagcaacgt ctgtcgctac
     5101 aaggatatct aaatgaccgc tcttaaatga acgcaatacg ctcattcgtt tttgttgaga
     5161 caaatcgcca tggattcctt ctgcacggta accgcgtgct tctaatccgc gtgccaattc
     5221 gtctacacgg cgttttgtac gtccgaagac gattgttaat tcaggtgttt gaacatcaaa
     5281 caagcgagtc atgatatcga atttttcgta ttcttttgcg cggacatagt attgatcgat
     5341 caagtcagct gtcatttctt tcgctttgat cttcacatga tgtggatttt tcatgaattt
     5401 aacgccaata ttcttgatag ctggcggcat cgttgctgag aaaagtaatg tttgtctttg
     5461 atcaggaact tgtgaaatga ttttttcgat atcttccaag aagcccatat tcaacatttc
     5521 atctgcttca tccaaaacta atgtttgaac ggttccaagt ttcaatgtgt ggcggttaat
     5581 gtggtcaagc atacgtcctg gtgtacccac aacgatatgt ggacgatctt tcaagccacg
     5641 gatctgacgg ccaatatctg ctccgccgta aacggcttgt acacgtactt ttttgtcgcg
     5701 ccctaaacga tataattctt cttgtgtttg gatagctaat tcacgtgttg gtgcgataac
     5761 taaaccttgt aattcgtgac gatctggatc gattttttct aacattggta atccaaatgc
     5821 agctgttttc cctgtacctg tctgtgcttg tccaataacg tcttttccag ctaatgctag
     5881 tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa ccagcgcgtt cgactgattt
     5941 caacaattct ggtgataatt ctagttcttt aaatttcaaa tgaatcctcc tatatggttg
     6001 ttaactgagg atgtagcttt tttgctaccc cgtcgatttg ttcggtcaaa gtgtattgtt
     6061 tctttttgac agtggccacg cacgttttta tccattctta gcccctgtgg caccaccgaa
     6121 aaatcacttt gcgaagttta ggcattagaa aagccaaacg ggtcgcgttc agctttatca
     6181 ttattatgac atctggcttc aacaagttgt ttctatggca aaaccagtcc aatttctctt
     6241 catctttcgg caataggtta ttctaacata gtttttcgtt ttctgcaagt ttacagattt
     6301 ttatttcatt tgctgaagtt gttctaccac ttcaaataag cccatgctgt tgctgccttt
     6361 tagcataata ctatctgttg cttcaagatt ttgtttgata gcatcaatca atttattctt
     6421 ttctttttca aaatagaaca caggtagttg ggggtatttt tggttcaatt cttcttttag
     6481 tgcatgcatt tgttccccat aaaggaaaat catggcgaat ttctcaggat cgatatgttc
     6541 actcatttgc gcatgcatat ttaaagactc tttccctaat tccagcatat ctgctaacac
     6601 agcgattctg cgtccttttg ttggcagatt tttaaaagta tctaatacca gtcccatggc
     6661 tgttggattt gcattgtaga catcgctcaa aatagctgca ccatttcctg ccgttagcca
     6721 ttcggttctg ttttgagtga tagaaacatg ggcgagccct tggcgtatct caggtatgga
     6781 cagtccaaag aagcgcccga tcgtatacgc aatcaaagca ttcgttacat tataacttcc
     6841 aggcagcggg atcgtaaatg tttctccttc tactacaaaa tcggtttgtg cttttccttt
     6901 tgctgtcact tccgcatgaa catcgccatt tccaataccg aatgttgtga ctgtttgtac
     6961 taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg ggcacaacga gtaatccgtc
     7021 tgatacaagt ccgtcagtga tttccatttt ggctttagcg attccttctc gcgacccaag
     7081 attttctatg tgtgcttcac cgatcattgt gattgctgca gcttttggtt gagccataag
     7141 tgaaagttcc gtgatttcat tggcgtgatc catgcccatt tccaaaacga tcttttctgt
     7201 atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata ttattgttgt aatttccttg
     7261 cgtcttatat gttttgtatt tttcagacaa gacagacgcg gttagatctt tcgtggtggt
     7321 tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct actttttcca agtaataggt
     7381 tgccaattgc tgcatggctt tcaaaggatc tgctacagga ataactggaa aattttttgg
     7441 agcttgttca acaggcaagc tccaaaatga agcagatgct ccgtttgtga tcgcttgctg
     7501 gataaattca tgtccatctc gcgtaccagc aagaggaacg aataaacttc cttcggttat
     7561 cttccggctg tcaaattcga cattggtaat ctttgtcagt gcagagcatt cgctgcccac
     7621 ggcttgtgca atttcttgta ttgttagatt cattgagatc tctcctaaaa aaggttgtga
     7681 aaaaagcgtt tggcctaaac ggcgggttcg catgactctc acttccaagt aaatttctct
     7741 attcgtattt agaagctaga atgaacgcgg ttacgcaatc actccagcct cttctttatt
     7801 tctctttatt catttttttc aaaaaaagat tgacgctgat ggtatcgatt cattcctaac
     7861 tggatcagtt cttcaatcaa atcaccgtat ttcaagccca tattttccca taagagtggg
     7921 tacatactga actccgtaaa tcctggcata gagtttaatt cattcaggaa taattcattt
     7981 ttatttgtca aaaagaaatc gcaccggctc aatccgcttc cacctaacat cgtgtaagct
     8041 aacttcgcgt actcttgcgc tttttgataa acttcttctg gcacttcggc tggaatctgc
     8101 atttcgattt tattattgat atattttgct tcataatcat agaatgctac gtcttttacg
     8161 acttcaccag gcaaagtcgt ccgaacgtct tcatttccta atacagcaac ttcgatttcg
     8221 cgcgcttcaa ttccttgttc aacgattgct cgagaatcat actgataggc tgttgctaaa
     8281 gcattttgca gctcttctcg gttttctgct tttgtaatgc cgacactaga acccatattc
     8341 gccggtttga caaacatcgg ataaagcaaa gaaccttcac attgatcaaa tactttttta
     8401 ggattttctt tccattgatt cttaagtact ggtacataag gaacttgcgg cacaccagca
     8461 gcttgtaaaa tatacttggt catgatttta tccatggcac atgcactggt caatacgcct
     8521 gcgccgacat aaggcatatt caatgtctct aagaagcctt ggatcgttcc atcttccccg
     8581 tttggcccat gtaaaactgg aaaaacgatg gctccttctt ctttgatttc gcccggattg
     8641 atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg ggtcccatga aagatgcaag
     8701 acatctttgc tggcaggttt ttctgttagt aatggacctt tgacccattg tccttctttt
     8761 gtaataaata cgagttgaac ttggtaataa ttataataaa tggcatttaa aactgaaaat
     8821 gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc catatagtaa agtaatcttc
     8881 aaaagattgt cctcctgtat tctaaatcgt tcagtgattt actctcatat tttagcacaa
     8941 aacaaaaata aacgaataac ttatttataa aaaaacgagc cagcttctaa aagaacgaac
     9001 gtcatttatt ttttgtcagc tagattattt gtcccgtttt ttataaaaag ttatgataaa
     9061 gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag ccgcttggca gaaatcattc
     9121 gagtgttcat caattacaat gtagtgccaa actttgtaca gcaaaaaaat cccgaacagg
     9181 taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa gataggacag atgctttctg
     9241 ttcgagaaga tttgctttct tctgccttca ctcaaacatt caaaacatta caagatagtg
     9301 tacctagcga cacattttca acggtcaaaa agacgatcga aacagaactt tctctttcat
     9361 tatcagatat attcgatgat ttttcaaaat cgccttttgc ctctgcttct atggggcagg
     9421 ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt taagatccaa caccctaaca
     9481 tcgccgaaga gatccgactt gatcttcaac tatttgaacg agcgatccct ttgatcaaat
     9541 acattcctga aactagcgta gttgatctaa aaggggtact ccaagaagta aaacgttcct
     9601 tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg cgaacaattc tatcaaaaga
     9661 acaatggctg gaaagagatc cgcagcccca agatttatga cgctttttgt tcgaaaaaag
     9721 tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca tttaatgaac gccgaaaaca
     9781 aaactgaaac ttttatcact ggaattcaaa acaagcagct gaaacaagaa gttgcaaagc
     9841 ttttagttga gaactttatg aaacaggtgt ttgatgatgg ttttttccat gctgatcctc
     9901 atccagggaa tctgctattc catgtactaa caaaagaaga acagacacaa gctagccgaa
     9961 aaactgaaac agtccatgaa aaagagttcg gttcctttgc ttttcgagcc tccacttctg
    10021 cagaagaccc tgttgcgcca tataccatca actacatcga ttttggtatg atggggcatc
    10081 tttctgcagg acttaggcaa aaactcacac aagccgtttt agcgctgtat acgaaagatg
    10141 cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca agaaggctcg tttgatgaaa
    10201 gtcggttcca ccaagaactg acaagttttc ttgaacagta ttatgattct cctattgatg
    10261 aaatcaactt gcaagaagta ttcacacatg tcgtgacgat ttgtcatcaa aataatctgc
    10321 agttcgatcg tgatatcacg cttttgctga aagcatttgg gacattagaa ggggtgatcc
    10381 gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc gccttttgct caacattatt
    10441 ttctcactca tctggatgta gaagatacat tgaaacaaag cggattagac ttattggaag
    10501 ggatgaaggc tgcgcctaag atccctcaac agctccacca tctattagaa atgtggactt
    10561 cgggacaagg aaaagtcaat ttggaattga aaaagcaaga caaattattg agcaggatcg
    10621 aaagtatgat caatcggctt gttttcggta tgattttagc tgcattgatc gtagggtcct
    10681 ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt cgtttcctta ttagggatat
    10741 ttacgtatgc tattgccgca ttcgtcatta tttttttggc aatcgatgca ttgatccaaa
    10801 tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga aagaatcagc ttcaagaaat
    10861 aagaaggggt tttacataaa ttgtttctca aatttttgtg aaaattcggc ttatttttcg
    10921 aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa aaagctgttc tgcacccagt
    10981 aataagaaca atcaaaaagg gaacatggaa cacatttatc ctgtttccct gttccctttt
    11041 tgatgtattc gagcataaat aaaatacctt cgttacctac tatcttctgt acttggtaaa
    11101 ccttagtcaa ttgccttttt taacgcttga cgaaaagaca tatgatcttt ctccgtaaag
    11161 ggttttggac cctttgtcct tatgccagct tttcttaact ttccactatg ataacgttgt
    11221 tctagaagcg tgccgatatt cgcttcgtca aattcttttc cagagtgatg gaacactcgc
    11281 acacccttcg gcaatacaag tgaagctaaa ccataatctt gcgtgatcaa aaagtcattc
    11341 ttcttaacca taccgacgat tttataatcc gcactatcag ctccgcggtc tacgtagacg
    11401 atttggacat gattagcgta ttctttggtc gtataatggt ccacactggt cacgataacc
    11461 acgtcaagac cataggattc tcctaatttt atgacatctt cttttactgg agaaccatct
    11521 ccatcaatga atagcctcat tctttttcgc cttcttccat aaatgataaa cagttactaa
    11581 tccgctcagt aaaagaccaa cattgatcca ttttgccaag ataagcagtt gtacactttc
    11641 tatcggcact gattcatggt tcagcgtgat caataataga tggattggct ctcccatttc
    11701 ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg tggatcacac tccctttatt
    11761 ttgagtagta tacttgacaa cgatccggcg atccattgga atagctgtat cagtttgata
    11821 atattcctta tttagccagc tgcctgttac tacgtgccag aaactaagca gaaagacacc
    11881 tatcgcaaaa ccatttggaa tcaagaggac caataaagaa gaactcagtc caagcaattg
    11941 catagccaat ggagaccatg ttaaacgttt caaaatccat ggtccgacta tttttgctgc
    12001 aatcaagcct gctgcataag gcaaatacat aaaaatcgaa agtttttcta atccgtacag
    12061 tatgccaaca taaaaactgc taaataaaaa aaggaaattc cctagcattc catttagaaa
    12121 agtaaacaga tttagccagc ttggcaactt ccattccttc ttctggtgtc ctaaaacaaa
    12181 cacgaacaag atgaacagca gcaagaaacc ataaatagca agatcaaaaa gcctttcatc
    12241 gaataacaat cgggcatttc tcaaaagaaa caataagcca aagaatatca aaaataaaac
    12301 aagctctcgc gtagctacca gatttctttt gatgtctttg aaatccagtt cgtaacgcgg
    12361 ataatgacta accgtatggt aggccatgac gtaaaaaagt gtataaatag taaagcttgc
    12421 tgcaatctga gtcggtgcag gcaactcaat cgcttgatac aggagaaata gccagatagc
    12481 tgcaaataag tactttttcc catccatatt gataaaacct tgcgtctttt cgtgaaaatt
    12541 gaccgtggta ttagcaggta caagccatgc tgcactcagc cctagcaaaa cacttgaaaa
    12601 ataaaataaa ggaaaataaa atacgcctaa tattccaagc aatgaaccag caccaccaat
    12661 cagcagagcg atcatcagca acgtgtaact atctagacca aatcgtagac tgttgactaa
    12721 aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat ggcaaaatcg tcagccaatt
    12781 atgcgagtct gttagtccca aaaataaaag ataaggaata aaaataaccg tgttacttaa
    12841 gataaagggc gttgtttgtg taaaattacg cccaaatcgt ttgagttttt cgaacatgac
    12901 aatggctcct taaacagtct tataactaat ctgctcttcg attttagcat taaaggggaa
    12961 ataaaccaat tttacagaat atattcttgt aaaaaaaaat acagaaagaa gcataagctt
    13021 ctttctgcat ttttacggta cttgattact caagcagaca agcccttctc cacaaaagtc
    13081 ttgcccgaca cccctgcatc cggcaggtga taaagataga aatcaattat cctgtgcctc
    13141 caaaatcaat taggagctct gtaaaccaga gtccttctcg acccgtcgca taaatttact
    13201 ttaacacagt tcctatgttt atgcgagagc ttttttcatt ttataatcga ccatttgaaa
    13261 tccttgaccg atatctgaaa taagttcttc tgtaatctca aatccttcat gcttataaac
    13321 ctcgattgct tgatgattcc cctgattcac tcgcaaaaag atctctcttt cctttgtttg
    13381 cgcagcaagt tcttcatacc agcgaaaaac tgaagaagtc aatccttgac cacgtacttt
    13441 atttagcaaa tataatttac ttaaataaat ttttccattt atctcttcat aagcagtgta
    13501 accgacagtc ttttcttcgc tttttaataa aaagtaggac acaccttctg tcagttcttt
    13561 ttgaatctgt ttttttgact gataagtttt cagcatgtaa tccacttgtt tttgaccaat
    13621 gatcggcaca aaaacttccg gccatacttc ttgtatgact gccatcagct catccagttc
    13681 attttctgtt acttttattt tttccataca cccacgtcct tttttacatt ataaatgacc
    13741 ttgcgcttga tggaataaaa agagcatgat catgctatac acacccaccg accacggttt
    13801 caagaaaatc acaatctgca taaattccct aaaagataat ttcgtcaaac cagctactag
    13861 acagatcaag tcgtctggcg caaagggaag cagcaaggta acaactagta cttttttaat
    13921 atctttcgtt tttcttgtca gcaattcatc aaatttctga tacttttttg gcgataaaat
    13981 aagctggaca aaggctacgc catagtaacg gacaagcaaa aatccgattg cttccccaat
    14041 cactaggcca atacaactat ataaaagccc aaatccattg ccaaacatca gcatgcccgc
    14101 tactgaggag atccctcccg gcaaaatagg aatgatcact tggatcactt gcaacagaac
    14161 aaatatcatc actgcatact tgccaaattg ctggataaac gcttgcaaag acgtgctcga
    14221 tcgaaaaatc ccatgatgat aagcataaag aatcaacaca aagaaaaaaa acagaccaat
    14281 aatgggcatg atctgaagac atttttgtaa aactgatcct tttttagaca tctatattcc
    14341 ccacatcttt tttcatttat taaaactgcc tcct
//
LOCUS       DACEDM010000074        14024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEDM010000074 DACEDM010000000
VERSION     DACEDM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.074"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IWO50_002277"
     CDS             140..460
                     /locus_tag="IWO50_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359377.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IWO50_002278"
     CDS             912..1112
                     /locus_tag="IWO50_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0359378.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IWO50_002279"
     CDS             1259..4048
                     /locus_tag="IWO50_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0359379.1"
                     /translation="MKPRTYAVIDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IWO50_002280"
     CDS             4095..4622
                     /locus_tag="IWO50_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0359380.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IWO50_002281"
     CDS             4643..5833
                     /locus_tag="IWO50_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0359381.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IWO50_002282"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IWO50_002282"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0359382.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IWO50_002283"
     CDS             8224..8898
                     /locus_tag="IWO50_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0359383.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IWO50_002284"
     CDS             8895..9236
                     /locus_tag="IWO50_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359384.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IWO50_002285"
     CDS             9266..9625
                     /locus_tag="IWO50_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0359385.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9828..10007
                     /locus_tag="IWO50_002286"
                     /pseudo
     CDS             9828..10007
                     /locus_tag="IWO50_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="IWO50_002287"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="IWO50_002287"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0359386.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="IWO50_002288"
     CDS             12541..14007
                     /locus_tag="IWO50_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0359387.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4649 a         2428 c         2997 g         3950 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcattgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gtta
//
LOCUS       DACEDM010000075        13919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEDM010000075 DACEDM010000000
VERSION     DACEDM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.075"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..507
                     /locus_tag="IWO50_002289"
     CDS             133..507
                     /locus_tag="IWO50_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0359388.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IWO50_002290"
     CDS             complement(621..1250)
                     /locus_tag="IWO50_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0359389.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IWO50_002291"
     tRNA            complement(1384..1456)
                     /locus_tag="IWO50_002291"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IWO50_002292"
     tRNA            1670..1743
                     /locus_tag="IWO50_002292"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
     gene            1698..1847
                     /locus_tag="IWO50_002293"
     CDS             1698..1847
                     /locus_tag="IWO50_002293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359390.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2340..3668
                     /locus_tag="IWO50_002294"
     CDS             2340..3668
                     /locus_tag="IWO50_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0359391.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3870..4703
                     /locus_tag="IWO50_002295"
     CDS             3870..4703
                     /locus_tag="IWO50_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0359392.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4719..5456
                     /locus_tag="IWO50_002296"
     CDS             4719..5456
                     /locus_tag="IWO50_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0359393.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5611..6579)
                     /locus_tag="IWO50_002297"
     CDS             complement(5611..6579)
                     /locus_tag="IWO50_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0359394.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6811..7644
                     /locus_tag="IWO50_002298"
     CDS             6811..7644
                     /locus_tag="IWO50_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0359395.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7678..8517
                     /locus_tag="IWO50_002299"
     CDS             7678..8517
                     /locus_tag="IWO50_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0359396.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8543..9103
                     /locus_tag="IWO50_002300"
     CDS             8543..9103
                     /locus_tag="IWO50_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0359397.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9302..11023
                     /locus_tag="IWO50_002301"
     CDS             9302..11023
                     /locus_tag="IWO50_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0359398.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11188..12330
                     /locus_tag="IWO50_002302"
     CDS             11188..12330
                     /locus_tag="IWO50_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0359399.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12346..13557
                     /gene="thiI"
                     /locus_tag="IWO50_002303"
     CDS             12346..13557
                     /gene="thiI"
                     /locus_tag="IWO50_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0359400.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2297 c         2834 g         4037 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc tttttctttt
     1801 attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga
     1861 gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac gaaataaatc
     1921 agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac attcccaaag
     1981 ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc aatcaggttt
     2041 ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat gagtattttg
     2101 aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca ggccattttc
     2161 gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta tgacattttt
     2221 cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag aagagattgt
     2281 atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag gatgattgga
     2341 tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa gcaaatatca
     2401 aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct gctgaaaatg
     2461 tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg gggaaagtgg
     2521 ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca ttccgtcata
     2581 agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac acttttttct
     2641 gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca ggaaaagagg
     2701 caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat ggcggccaat
     2761 gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg
     2821 aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa ttactgcgta
     2881 aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac gctattgaag
     2941 aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca ttaggaacac
     3001 ctccaaaaga attcgatttt gagtaccgcg atgaagaaaa gaactatcat cttgaccgag
     3061 gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat gattatgtta
     3121 gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca gtagagatgc
     3181 taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg gaaactttca
     3241 aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt tgtgatgtag
     3301 gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca atggatgatc
     3361 tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga gaaagtctga
     3421 tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca acaaaatgga
     3481 aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg
     3541 cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg acgaaagagc
     3601 aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca atgggtgctt
     3661 tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag ctggcttaag
     3721 ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt gctataattc
     3781 taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga tgacgaagta
     3841 gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta tctgatctga
     3901 aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc tatcgtataa
     3961 aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg
     4021 aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg gatacgtata
     4081 tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag ccgggaaaag
     4141 gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca gcgccacaat
     4201 ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct tttgaagaag
     4261 attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg gcagaagaaa
     4321 cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga
     4381 aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg tggagtggta
     4441 atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt tcttatggga
     4501 atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg gaatttttag
     4561 atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg ccgatttacc
     4621 agttatatta tcttttggca catttgaata tgtttggaga aagttacggt tcccaagtag
     4681 atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa
     4741 agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt
     4801 taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga ggcagtgaat
     4861 tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag attaatcgtc
     4921 tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac aataagtttt
     4981 tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc aaaatagaat
     5041 ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat gaagagatca
     5101 ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga
     5161 ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat agtaatcagc
     5221 atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat gactttttga
     5281 ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag tacgggaatg
     5341 agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa
     5401 tccgaatgca agaacagctg tattgtgaag caaatattat atggaaaaat aaataaaaaa
     5461 gaggttgtga cagaaggggg caacttcaag aaataagaag aaatttacga aaattgtttc
     5521 tcaaattttt gtaaattttc gacttagttt tcgaagaagc tatttttgtc acagcctctc
     5581 tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa tcaattcttc
     5641 acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt gactattaat
     5701 actgttacag aaaataatcc ccattttttt tagttctttt cccccgcctt cataatcata
     5761 aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga ttttttggac
     5821 aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac ctaaagcttc
     5881 gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc cttgcatccc
     5941 agcatatgct ttaacgatcg ggatagtccc atctacagcg gaaatgtcca gtcgctttgt
     6001 ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga tgggacccaa
     6061 tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga
     6121 atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt cacaacttgc
     6181 gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat tacttgaacg
     6241 cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc
     6301 agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac ctgatgcata
     6361 agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg
     6421 aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga tttctgcatc
     6481 taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt ctttcatggc
     6541 gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt ccttacgaat
     6601 aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg ctatcagtga
     6661 agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat ggaaaagact
     6721 caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca agaaagcagc
     6781 caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac atcgtttaaa
     6841 gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc agggtttcta
     6901 cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc agttgagaat
     6961 tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca aggaatcagc
     7021 ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc gcatttttta
     7081 gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt ccaattcccc
     7141 tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag aaagtggttt
     7201 gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga tgaatttctt
     7261 gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat cgatgagcca
     7321 cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa ctttgctctt
     7381 tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa
     7441 aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa
     7501 gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc tggtggtgat
     7561 gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga tggactcaat
     7621 ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc gaagtctatg
     7681 atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc aaaagcgttg
     7741 gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg cggtgtagca
     7801 acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt agatatgtat
     7861 gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga ccaccctttt
     7921 gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg acaaatcgtt
     7981 tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca atcgattttt
     8041 atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc
     8101 ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga gatattaaaa
     8161 gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa gttagaacat
     8221 cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat catcccaaaa
     8281 ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa
     8341 gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg tgtaggaatt
     8401 ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa
     8461 tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat ttattagtca
     8521 atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag tagaaacatt
     8581 ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag aaaatgaagc
     8641 tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag ctacttcacc
     8701 taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag ataaagcaga
     8761 agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac aagtagatgc
     8821 actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa tagtgcctga
     8881 tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc
     8941 tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc
     9001 tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa
     9061 gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt
     9121 ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa
     9181 aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt
     9241 atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata
     9301 aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt
     9361 ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa
     9421 acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat
     9481 gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat
     9541 atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga
     9601 aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca
     9661 aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga
     9721 acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt
     9781 cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt
     9841 gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc
     9901 ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa
     9961 aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag
    10021 tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga
    10081 agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga
    10141 agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga
    10201 tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc
    10261 agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac
    10321 atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga
    10381 agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat
    10441 tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga
    10501 aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc
    10561 gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa
    10621 acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg
    10681 tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg
    10741 tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt
    10801 agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca
    10861 cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta
    10921 tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa
    10981 acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa
    11041 accagcaaga gcaagacgga ttattgtctt gttcttgtct ttttttgtat atttgaaaat
    11101 tgaaacaagt gaaaatttca cctgctcttg aaagcacctt ggacaaccgt tataatagaa
    11161 aagaatttca aaaaagaggg gtttcttatg atatattttg ataacagtgc aacaacccca
    11221 atctttccgc aaagcttaga tgcatatgta aaaaccagtc agcggattat tgggaatcca
    11281 tcaagcttgc atgatttagg caatcaagct aaccggttgt tgcagcaagc aagaaagcag
    11341 atcgctgatt tgatccatgt agaaccagaa gaaatctatt ttacttcagg cggaacagaa
    11401 ggagataact gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg
    11461 atcatttcag gagtcgaaca tccggccgtc agcgagacag cagaacaatt aaaagaatta
    11521 ggaatcgaag taagtatcgc cccagtggat aaacgcggat ttgtacgtgt cgacgaattg
    11581 aaagaattaa taagaaaaga gacagtatta gtctctgtta tggctgtgaa taatgaagta
    11641 ggatcgattc agcccatcca ggaaatcagt gatcttttgg atgattttcc taaaattcat
    11701 ttccatgtag atgctgttca ggcgattgga aaagtaccat tctcgcaatg gttgacagaa
    11761 agagttgatt ttgcaacatt ttcagctcat aaattccatg gcccacgggg aactggtttc
    11821 atttattgga aaaagggtcg tcgtttagca ccattactga caggtggagg gcaagaaaga
    11881 aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg ctatggttaa agctttacgt
    11941 ttatcattag agataaagga acaaaaacct gagcagacac aacgcttaaa agtagctcta
    12001 atcaatgcgt tgaagacgta tgagaaagtg acgatttttt cagaaggaac tgactatgca
    12061 ccgcatattt tatgttttgc tctaaaagga attcgcggcg aagttctcgt ccatgcctta
    12121 gaagaaaaac aaatatttgt ttctactact agtgcctgct cctcacgtaa acatatggca
    12181 agcagtacat tgcacgcaat gcatgtacca aatgatctgg ctacatctgc tgttcgaatc
    12241 agcttagatg aaaacaattc gcttgcagaa gtcgagcaat ttatgattat tttcaagcag
    12301 ctttataaga aattttctgc aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa
    12361 attatggtcc gttatgggga actttcgaca aaaggaaaga atcgaaaaag ctttatcatg
    12421 caactagcac aaaatgtcag acaagctttg aaagactttc cagaaatcaa aatccatgct
    12481 gatcgggacc ggatgcatct tttattgaat ggcgcagacg ggcagctagt tattccaaaa
    12541 ctagcaaaaa tatttgggat ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc
    12601 gaagttttga aacaagctgt tcaagacatc atgaaagaaa tttatactgg gaaagaaaca
    12661 tttaaaatta tcgctaaaag aagcgatcac caatttgaat tgacaagtaa tgaattgaat
    12721 caaacattag gtaatgctgt ttttgatatt ttcccgcata tccaagttca aatgcgtcaa
    12781 ccagatattc cattacgtgt agagattcgg cgtgatggcg cttatctatc gtatgaaacg
    12841 atcaagggag ctggaggatt gcccgtagga acaagcggaa aaggaatgct tatgctttct
    12901 ggggggattg attctcctgt agcagggtac tttgccatga agcggggagt ggaaattgaa
    12961 gctgttcatt ttgctagtcc gccgtataca agtgaacaag cactgcaaaa agcaaaagat
    13021 ctaacagcca aactagcacc ttatgtggga acgatccatt ttatcgaagt gccatttacc
    13081 gagattcaag aagaaatcaa aaagtcagtg ccacaaggat attggatgac aattactcgt
    13141 cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt agtcattttg
    13201 aatggtgaat cattaggtca agtggcttca caaactttgc aaagtatggt ggctatcaat
    13261 gaagtaacga atacgccaat cattcggcct gtagcgacaa tggataaact tgaaatcatt
    13321 gaagtagcag aacagatcga tacatttaat ctttctatcc agccttttga agattgctgt
    13381 acgatttttg ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat
    13441 gaagagcgtt tagcaataga tgaaatgatc caacgagcac tagctggctt gaagatggaa
    13501 gagatacaag cagaatcgtc taatgaaaaa actgaagaat tttcagaatt gctataagag
    13561 gttatgaaaa tagtgttttg accagagtat tagagaaaca aaacgaaaaa atagctccat
    13621 gtattttacc gtttttgaac taatgtcgaa gattagctga ttgaatacca tttatcaaga
    13681 agttatggaa aagccggttc ttttttacta actgaaaaat aaaattttat ttagagatca
    13741 agctacagtt gataaatgat ataaaaaaga gaatttccca tgaaatagcg aggttgtgac
    13801 agaagtgggc agcttcaaga aataagaagg aatttacgaa aattgctttt caaatttttg
    13861 tgaaatttcg gcttattttt cgaagaagtt acttctggaa caccgtgtat cagaggttg
//
LOCUS       DACEDM010000076        13833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEDM010000076 DACEDM010000000
VERSION     DACEDM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.076"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..364
                     /locus_tag="IWO50_002304"
     CDS             <1..364
                     /locus_tag="IWO50_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359401.1"
                     /translation="FIPEDFQNLQLENMRLEDLFRLADDDKYRFEVLKIKKNELLNKL
                     YPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYVEDEVI
                     IKYMKPVIKLVNKNKGNG"
     gene            470..733
                     /locus_tag="IWO50_002305"
     CDS             470..733
                     /locus_tag="IWO50_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0359402.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            733..1005
                     /locus_tag="IWO50_002306"
     CDS             733..1005
                     /locus_tag="IWO50_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0359403.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1082..1411
                     /locus_tag="IWO50_002307"
     CDS             1082..1411
                     /locus_tag="IWO50_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0359404.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1401..1688
                     /locus_tag="IWO50_002308"
     CDS             1401..1688
                     /locus_tag="IWO50_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0359405.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1974..2831
                     /locus_tag="IWO50_002309"
     CDS             1974..2831
                     /locus_tag="IWO50_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0359406.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            2832..3380
                     /locus_tag="IWO50_002310"
     CDS             2832..3380
                     /locus_tag="IWO50_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359407.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(3677..4624)
                     /locus_tag="IWO50_002311"
     CDS             complement(3677..4624)
                     /locus_tag="IWO50_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ0359408.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            4812..5999
                     /locus_tag="IWO50_002312"
     CDS             4812..5999
                     /locus_tag="IWO50_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ0359409.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            5980..7284
                     /locus_tag="IWO50_002313"
     CDS             5980..7284
                     /locus_tag="IWO50_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0359410.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            7310..8323
                     /gene="citC"
                     /locus_tag="IWO50_002314"
     CDS             7310..8323
                     /gene="citC"
                     /locus_tag="IWO50_002314"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ0359411.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            8327..8620
                     /gene="citD"
                     /locus_tag="IWO50_002315"
     CDS             8327..8620
                     /gene="citD"
                     /locus_tag="IWO50_002315"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ0359412.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            8625..9530
                     /gene="citE"
                     /locus_tag="IWO50_002316"
     CDS             8625..9530
                     /gene="citE"
                     /locus_tag="IWO50_002316"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ0359413.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            9523..11082
                     /gene="citF"
                     /locus_tag="IWO50_002317"
     CDS             9523..11082
                     /gene="citF"
                     /locus_tag="IWO50_002317"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ0359414.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            11079..11636
                     /gene="citX"
                     /locus_tag="IWO50_002318"
     CDS             11079..11636
                     /gene="citX"
                     /locus_tag="IWO50_002318"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ0359415.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            11629..12492
                     /gene="citG"
                     /locus_tag="IWO50_002319"
     CDS             11629..12492
                     /gene="citG"
                     /locus_tag="IWO50_002319"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ0359416.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            12726..13135
                     /gene="tnpA"
                     /locus_tag="IWO50_002320"
                     /pseudo
     CDS             12726..13135
                     /gene="tnpA"
                     /locus_tag="IWO50_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         4953 a         2052 c         2721 g         4107 t
ORIGIN      
        1 atttataccg gaagattttc aaaacttaca gttagaaaac atgcgtttag aagatttatt
       61 tagattagca gatgatgata agtatagatt tgaagttttg aaaattaaaa agaatgaact
      121 attgaataag ttatatcctg agaatgcgat aaaagcacat tttggtgatg ctaatcatat
      181 gtatagttgg ttcattccta aaagatttag agggaaaatc aatgctggag atcaagtgaa
      241 agtactcaat cgaaatagta cagatgtcgt aatgataaca gatatctacg tagaagatga
      301 agttataatc aaatatatga agcctgtgat aaaacttgtt aataaaaata aaggaaacgg
      361 ataacacttt acaatgctgt tttggtactc caatttaaac aaaacaatcg aatttacgta
      421 cttaattaag tacgttagaa tggagatata aattagaggg ggagtagata tggcaaaaac
      481 agtcttaaat cctagctcag cacggaaaaa tttttataag ttgttgaaag atgtaaatga
      541 gaatcatacg gaaattgaaa tcattagtga gcgcagtgaa aataatgcgg tgcttattgg
      601 tttggatgat ttgaaagcaa tcaaagaaac gctattactt gagcaaatag gcactttggg
      661 tgttgttcgt aagcgagaaa aagatgatag tggatttact aatgttgatg aaattgattg
      721 ggataactta taatgaataa ctattctgtg atgattaaaa actcggctaa agctgattta
      781 aagaaaatca aacagtctaa tttgaaacct caatttgaga aggttattca gactttaaag
      841 gaagatccct atctccctac ccaatcattt gagaagttaa aacctacata tgaaggtagg
      901 tattctagaa gattgaatag acaacaccgg gttgtttata aggtagatga agatgaacac
      961 gtggttgaaa tttattcagc aaggacgcat tacgagtcac agtaattact tgtaaataca
     1021 ttatctcgtg gtataatgta tttactaagt aattacatac tgttagaaat gagggattta
     1081 aatgattaca aaaacaagaa aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt
     1141 taatgttcct aatggagtcg aagttgaagc aaagttggta gagaatggta ttctttatga
     1201 atttgttgaa ccacaaaaag aattttatga ttttagcgaa gatattttgt ctgatataat
     1261 tgcagaaggc tacgataaag atgagattct tgtagaattt aagaatagaa aaaacaaaat
     1321 gcattcttcc tttagagata ttgctgaaga tacacttact aattctaaag taatgactaa
     1381 agaggagttg gcaacagaaa ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt
     1441 atttaaaaaa gctaaaagag aagccattga aagaaaaatt tttgaattta atttatgatg
     1501 aaattgcgat tgatccatat aagggtgaac aaaaaactgg ggatttatca ggtatttggt
     1561 ctagtgggtt caaatatgcg ggcacaacat atagagtagc ttacgagatt atagaaaaca
     1621 aggttattcc agtattgtta tgtggaacgc atgaaaactt ttatgaacag ttgaaaaaat
     1681 taatctagtg atttgttgtc gttcttaaga aggacggctt cttttttatc gaaaatctga
     1741 ttcgtctaca tgactaaagt cgcgagtatt ctctgcttct cgataaatta aataacgtca
     1801 ttactttgag gaaagctata aatcaatata tttcaacttt tccttattta tgtactacct
     1861 tttacttatt aattataagg tagtacatat atatcttttt tttggtagta actaaaaata
     1921 aatatgttat aatagtatca aatgaatatt gctaaaagaa aagaggtagg aacgtggtag
     1981 cgaagaaata tgtaattggt aattttaaag gtggcgtagg taaaagtacg tgcgcgcaaa
     2041 tgttagggtt tgaatcagca gttaatgaag gaaaaagaac cttgataatt gacttggata
     2101 tgcaaggaaa tacttctgat gtgatgaact taacgcatat gaacttcact aacgaagaag
     2161 gaggcggaga aggtgaaccg attgtttacg aaaatactgt gactgacgtg ttgataaaca
     2221 atcttccacc taaagaggct gtctataaag taatcaataa cctctatata cttccagcag
     2281 atatgtcctt tgaaatgtat gatgattgga tcaaagacca atataatgat tcattatccc
     2341 aattcaaata tatgaaagaa aagctagaac cattatttga tgagtttgac gtcatctatt
     2401 tagatgtccc accttctatt tctgtatata gtaaatccgc gatgtacatt gcggactggg
     2461 caatcgtcgt cttacagacc caagtaaaat cgatgagaaa tgcgatgcaa tatcttgagt
     2521 atatggactt ttttgtgaaa gaatttgata caaatttata tgttgctggt gttattcctt
     2581 tcatgttgga aagcggtgac gcagtagatc aggaaatgta taaacaagcc caagagattt
     2641 atggggaaca tttgattaaa aatgttgtac tcaaaaatgc gcgcttaaaa cgatacgacg
     2701 gatcaggtat tacgatggag aagacaaaga aaggtctctt aaaacaatgg gatcgcaaaa
     2761 gccacgaatt atttatcagt attttgaaag aattggaaga acacgagaat tggtatgcgg
     2821 aggtggagta attggctgac atcaaaggac ttgatatttt tgggaacaac aaaggactcg
     2881 ccaaaataaa aaaagaagca gaagcaaaga aaaagcagca agcaattaca ccatctgaaa
     2941 ataagcaatt agaagtgcac caagttcaag aaataaaaac ttcctccaat gttgaaagac
     3001 agagtagagg aagaacaaaa aaagttggcg caccaatcag aaagtttgat aaggtttatt
     3061 ccaccaagca tcctttaaaa ctgtcgactt tgttaaatgc gacttccaga gttttagtag
     3121 aaaagtatga gactaatatg acaagagatg aattgcttag aaaagcttta gatgagtata
     3181 ttaaacagaa tcttacgcaa gaagataagc aagatttgtt taatgacgtg gtgaaagaac
     3241 tagacttgtt cagagaaaaa tatccaattg ttccagaatt agatgaccgt ggagaaataa
     3301 taagaactac cagtcagata aaacaagata ccgaagactc actgcgcaaa gggtggggaa
     3361 tgaataataa agttagatag aacccccttt ttatagcata aaaaagattt agaaggatat
     3421 atgatcctat tgcatgagaa gtacaataaa attaataaga agtaacatta acgaaaaaac
     3481 gtggaaatta ttcagtgaat acaaagaaat taaatcttga aaagtaatgg agtatggctt
     3541 ggaaaagttc aaaaaatatc aagacaagct tctcaaaaaa agggattttg atatccaata
     3601 aaaagagtga atcattgtac tgccctccaa aaaagtagta ccaaaaatct aaattttttg
     3661 gaggatttta catgactcac tcttttttta tcaaagaaag tattttccaa gcagtttttt
     3721 catccaaaaa taaatctgta aaaaaattac cattcaaggc gcctaaacaa ctctccgctt
     3781 tgaacttact tttacaaata ccaattcgtt ttggaatatc gaaaatttct tttaaagaaa
     3841 gaccaaaagt cttatcctca gatgctttga tgaattcacc attgatattg aagggtcttc
     3901 cgtaaagaaa tccaacagta tccgtcatgc ttggaaatag gattttacga tgcttgttcc
     3961 agggttcgac agaatcaata gaggataagg tgccaattcc agaaattaac atatctgcat
     4021 gcgaagcggt tgctaaggta ttagctaaag aaggttctag agccattagt tcacgtgcag
     4081 tttcattgat gacatataaa ggaatactga gagtcaaata agaagactca taacgttcag
     4141 cagctttttg aactaatcgt gtggagccag ctaaggaatt atattttccg atttcgccaa
     4201 tgaattgagt aaaaattaat tcttcacgag cacccatttt aaactgatca atcactttgt
     4261 aaacggtatc tccccaagat agtgcaacca cattggcttt cttaattaat tcttgaatgt
     4321 aatcggcagc aaattttgaa aataataaat cgtgttgtga taagtcttca ctacttttta
     4381 atatatagat attcgtttta aagtaatttt tgaagatttc ttctagctca taattcctag
     4441 catacggaga attaattgaa atactaacta attgtttttc acgtgcatct tcaatatatt
     4501 ttaaagcttt atatctggtg atcccatatt ttttagaaat ttcactaata ttgatatcct
     4561 cgagataata gtcttcacaa atttttacaa ggagtgattc atagtcagag aattccagat
     4621 gcatagtata gctcctaact ttttcaaata gatatttttt ctcattatac caaaaaaatt
     4681 gaatttagtc tttttttaaa tttttaaaaa taagcatcat ttttttaaaa tttaaaatga
     4741 aagggttttt ttattttaaa aaaagtgtta aagtggtttt gtaattaaaa aatgtaaaaa
     4801 ggagtgaagg tatgcttgat gaagttttag aaatccataa aaaaaatgta ggtgttttac
     4861 ggatggaaac aacagaagcc gtgcaagatg atcaagattt agcaaaaatg tacaccccag
     4921 gtgtagcaga actaagttta atgattgaga aaaatcatgc tttggcaaga gaattaacaa
     4981 ttagtggtaa attagtggcc gttattacag acgggtcggc ggtattaggt cttggaaatg
     5041 taggaaacca agctggatta ccaatcgtcg aagggaaagc gctgctttat aaaaatttag
     5101 caggtgtcaa tgcgattcca ttagccatcg aacaaaaatc agtagacgag attgtacaaa
     5161 caattgtcaa tttgcaaaat agttttgcag ggatccattt agaagatatt gcagcaccga
     5221 agtgttttga gattgaagaa aaattacaag aaaaactttc tattcctgtg tatcatgacg
     5281 atcaagaagg aacagcgata gttgttttag ctggattgat caatgctgct aagatccaaa
     5341 ggaaaacatt aactgaactt cgagttttga tcaacggcat gggagcatct ggggttgcaa
     5401 cagctagact attaatcgct gctggtatca aaaatctaac attggtggat aaacaaggga
     5461 gattaaagaa gcaagatagt acattaaata aatatcaaag agagttgttg gatcatatac
     5521 atgatacaaa taataatgca agcgttttgg aagatgcaat cgttggacaa gatgttttta
     5581 tcggtctttc aataggaaat gttttaacgc ctgaaatgat tggaaaaatg aatgataatc
     5641 cgattatttt tgctttagcc aatccagtac cagagatcac acccgattta gcaaaagaat
     5701 ctggtgcaaa gctaatcgct acaggttctt cgaaatatcc taaccaagta aacaatattt
     5761 tagtatttcc cggattgttc aagggactat tactatcgaa agcgaataaa gtcgatcttt
     5821 cattacaact tgaagtagcc aaacaattag ctgctaaggt cgcagagcct acggtagaaa
     5881 aattcatccc taatgttttt gatgaaggag tcgcaaacgt cgtttcacag gcagttttgg
     5941 attatttaca aaaactaaaa gaaaacgagg ataatataca tgctgaaaac attaaataga
     6001 attaaaattg gttttattcc tttatctctt tatatcattt tggtactcgc gtatcttgca
     6061 atgacgataa gcaatatcgt tccagaaaat gatatgttag tttctttggg tattatgact
     6121 gtttttgctt atttattaga agaaattggt aaaactattc ctattttaaa agatttaggt
     6181 ggtaaagtat tagttgttac atttttgcct gcatttctag tctataaaca ttggctacct
     6241 caaagtactg taactgtggt ttctgatttt atgaataata acaatttctt gatgttcttt
     6301 atcacactac tagttgtcgg cagtattgct tcaatgaatc ggcaaacatt ggttaaagca
     6361 acttcacgta ttatcattat tttaatttta tgcgatgtga ttggttcttt gataggaaca
     6421 ggtgtgggaa tactccttgg gctatctgct tggaatgcgt atttctttat cgttgcgccg
     6481 attatggctg gtggtgttgg ggaaggagcg atgccattat cgattggtta tgcagcaata
     6541 ttatctggta tgggacaaaa tgatatcttt gggaagattc taccgtgtgt gatgttggga
     6601 agtttagtcg cagttctact gtctggtatg ctcaaaaaaa ttggagagaa atacccacaa
     6661 ttaaatggtc agggtaattt gatcgatggg gatgatgaac atttagagaa cttgaagggt
     6721 tcagctaaac cagtcaatat tgaaaaaaca ttgttagcag gcgtggtagc aataacgatt
     6781 tattttgttg ctgtctatgt tgagcactta ttaaaatcac tccttggctt ttctattcct
     6841 tcacctgtga tcttattagt ctctcttatg ggggctaaaa tgcttggttt tatccctaaa
     6901 aatatcgaag aaggtggaaa ttcgttgtat ggattcactg ttcgtgggat cacacctccc
     6961 ttactttttg gcgtaggggt tgcaaaaaca gattggttag agctgatcga agtatttaaa
     7021 aatcctgcaa cgatagtaac aatcattgtg acggtagtag ctattgtcgc tacagctttt
     7081 atcgccgcaa aatttttaaa aatgtaccca gttgatactg ccatcgcagt ttcatgttgt
     7141 agcggacaag gcggtacagg tgctttagct atcttagctg cgggtgatcg catggaatta
     7201 atgccatttg cacaagttgc tgttcgacta gggggtgcta tgaccgtaac gttcgctatc
     7261 tttttaatgg gtctgctatc ataagaacat aaagaaagga cgtgaccatg tgtttgatca
     7321 aacagatatc caacgattat ttttaaaaaa taactatatc aaagaccaat gggaaaatct
     7381 tttgcttgat tcaggaatac aaaagaagca aatcgaagat tttcaacatt tagatcaaac
     7441 attgggtata taccataaag aaaagttagt aggaacagta tcttatcaaa acaacgtgat
     7501 taaatatatc gccgtgtctg aaaaatataa agatggtgga catacattta atgtactagt
     7561 ctctgcaata attcaggcaa tgagtcaaca agagattttt cattatattg ttttcacgaa
     7621 accgcgctat caaaaaagtt ttaacttttt aggttttgaa acaatcgtat ctaccccaaa
     7681 aatagctgta ttagaaatgg gagatagttc tataaaacag tacctagaga ggattcctaa
     7741 agtgaatggt gaaaaaaaag cagcaattgt catgaatgcg aacccattta cagaagggca
     7801 ttattattta gtaaaacaag ctgccaataa caacgattgg gtatatgtat ttgtactcga
     7861 aaaagaacaa aatctttttt cgacgaagga ccgactcaaa ttagttcaac aaggattaca
     7921 aaaactgaaa aatgtcattg ttctatcagg tggcgagtat attattagcc ctgcaacgtt
     7981 tcccacctat tttttacgag aagatgatga aatagcaaag gagcaaatgg tcgtagatgc
     8041 tactttattc aaagaaagaa ttgccactta tttagatatc tctgctagat atgtgggaga
     8101 agaaccactt tccccaatga ctcgttcata taacgacata ctggaaacga ttttaccccc
     8161 tgaaataaaa gttcatgtcc ttgcaaggaa gaaaacggaa caacaccaag ttatttctgc
     8221 ttctcaagtt cgtaaagcgt atcttgctgg gcaattggaa aaaataaaat atatggttcc
     8281 agaaacaacc tatcaatact taaaaaataa aagggagcgt taagtaatgg aaataaataa
     8341 atcagcgatt gctggaacat tagagtcaag tgatgtccaa atcatgattt caccgaatac
     8401 agaaggaata gaaatcgatt tagacagtca agtgattgag caatttggaa aacaaatcaa
     8461 aaaagttatc tatgacacac tagcagttta tcagattaaa aacgtaaaac tcaaggtagt
     8521 agataaaggg gcattagatt gcgttattgt tgcacgatta aaaacagcga tccatcgttc
     8581 cttaggtatc caacaccaag aaacaccgtg ggaggtttaa ttagatgatg aaagaacgtt
     8641 tgagaagaac aatgatgttt gttccaggaa ataatccagc gatgatcaaa gatgcaggta
     8701 tttatggtgc tgactcgatt atgttcgacc tggaagatgc agtgtctatg gcagaaaaag
     8761 acagcgcaag aatgcttgtg gctgaggcat tgaaaacagt ggattatggt gacacagaat
     8821 taattgttcg aataaatgac ctgcactcag aatttggacg tgcagacatt tttgcaatgg
     8881 ttaaagccgg cgcgcatgta ttacgtttac ctaaaacaga aacctctgaa gatataacag
     8941 aagtagaaaa agtgattata gaagcagaaa actatcataa tgttccgata gggcgaacat
     9001 tgatgatggc agccattgaa ggtgcttctg gtgtattgaa cgcacctgcg atcgccaaag
     9061 catctgatcg attgattgga atcgccattg gggcagaaga ctatgtgaca aatatgaaaa
     9121 ccaaacgata tccagatgga aacgaattgt tttttgctcg cagtatgatc gttcatgctg
     9181 cacgtgcagc aggcatttcc gcatttgaca ctgtttactc tgatgtggat gatatggaag
     9241 gatttttaaa cgaagtacgt ttaatccatc aattaggttt tgatggaaaa tcttgtatca
     9301 atccacggca aattccagta gtcaatactg tttatcagcc gtcaatcaaa gaaatcaaac
     9361 gatcactaga catcatctcg gcaatcgaag aagcaaaaga aaaaggttct ggcgtaattt
     9421 ctttaaatgg aaaaatgatc gataaaccaa ttgtagaacg tgcacaacgg acattgatgt
     9481 tagcaaaagc atcacgattg attgatgagg agggaaatat ccatgcataa taaacaaaga
     9541 cgtagcattc catcaagtgt tttaacaaag tataaaaaag aattccaaag tgtttcatta
     9601 tcgccacaaa cgattcaacg ttgtgcacca aaagtaacga ctgtaactaa tcagacaaat
     9661 aaattgatgc actcgatcaa agaagctata gaaaaatcgg gcttaaaaga tggaatgacg
     9721 atttcatttc atcatcattt gagagaaggt gattttgttt ttaaccaagt gatggccgtg
     9781 atccgtgaaa tgggcatcaa agaattgacc ttagcaccaa gttctttaac gaacactatg
     9841 aatgagatgg ttattgaatg catcaaggac ggtacagtaa ctcatatcac aagcagtggg
     9901 atgcgcggca gcttaggtga atttatttct catggaggaa tggacaatcc tgtgatttta
     9961 cgttctcatg gtaatcgtcc acgtgcaatt gaaagtggtg aattgaaaat cgatgttgca
    10021 tttctaggag taccaacatc tgatgattat ggaaatgcta atgcgactgt tggaaaatcc
    10081 gtttttggtt cattagggta tgcaaaagta gatgcacaat atgcagataa agtcattctt
    10141 ctaactgatc atttagttcc ttaccctaat acgcccatta gtattccgca aacacaagtc
    10201 gactatgtcg tggaagtaga agcaattggt gatccgaata aaatcggtgc tggtgctaca
    10261 cggtttacta aaaacccaaa agaattaaaa attgctgaaa tggtcaaaga tgtcattgtt
    10321 cactcacctt atttcaaaga tggcttttct tttcaaacag gaacgggtgg tgcagcttta
    10381 gcagtaacgc gttacttagc tagtgaaatg gaagaaaaac agatcaaagc ttcttttgca
    10441 ttaggtggca tcactaaacc aacggttgat ttattggaaa aaggtcttgt tgaaaaagtg
    10501 ttagatgtcc aagactttga caaaggagca gctgaaggaa tggcgcggct tgagaaccaa
    10561 caggagatcg atgcttcctt ttatgctgat ccaagtaata aaggagcggt tgttaaccaa
    10621 ttagatgtcg ctattttatc tgcattagaa attgacacaa acttcaatgt aaatgttatg
    10681 actggttcag atggtgtgct aagaggtgcg attggtggac atcaagatgc agcaacggct
    10741 aaacttacta tcatctcagc gccgttagta cgtggccgaa tacctacagt agtaaatgat
    10801 gttactacat tgatcacacc agggaaaagt atcgatgtat tagtcacaga agtaggggtt
    10861 gctatcaatc cccaacgtaa agatctaata gctatttttg aaagaattcc acaaattcct
    10921 gtttttacaa tagaagaatt acagcaaaaa gcagaaaaaa tagtaggcat cccggaacct
    10981 ttgcaattca cagatcggat agttgcctat gtagaatacc gagatggttc aattttagat
    11041 gtcgttcgtc aggtgaaaga atttgaagaa gagagatcat gatgtttgat actggagaaa
    11101 ttcaagaaat cgaggatatt ttgatccata aagaacaacg tgctaacttg caaagcaacc
    11161 tgctttccaa gtattcaaat agttcattga tggtctttac attgaatatt cctgggccga
    11221 tcaaaaataa tccaatgatt gagcaggtat ttcgtcaggg acagcagtca attcagcaaa
    11281 tgtttttaaa aaaaaaggtg gaaatgacgt acacaaaaga aatcaatttg ccaagtggat
    11341 tggactggta cgtaatagta acagaagcac ctttgaatct caaaaaatgg ttggtcacgt
    11401 tagaagagca agacgcatta ggtagacttt ttgatttaga tgtcttgttc aaaaaaggag
    11461 agcagataac aattgtcagt cgccaagctc ttaatcatgg ggaacgtcct tgttttattt
    11521 gtgggaaatc agcaaaggta tgtgcacgta atcgtacaca tactgtgaaa gaaatgcaaa
    11581 agaaaatcga acaaatagta gccgattcct taaaaggagc attgaaacat gcatgataat
    11641 attcaaaaaa tagcacaaga agcacaatta gcgatgcttt atgaagttac atgtatcaat
    11701 aaaccaggat tggttgatcc agtagacccc gggtctcatc aagatatgga catttttact
    11761 tttctacaaa gtagtgtagt tttgactccc tattttgaag aatttatcca aacaggattg
    11821 gaccgacaac atcaaccaat tgaagaaacc tttatggcga ttcgacagat tggtttagag
    11881 gctgaaactg cgatgttctc agtgacaaat ggtataaata cacataaagg tgctatcttt
    11941 tcattgggga tttttcttgc tatttgtggc cggttaacta tttggaaagt tccttgtaaa
    12001 aaaagtttct ttcaaaaaga aattcaaaaa atgacgaaaa atctacttaa cgattttggg
    12061 aagattgatc aaacaaaacc aaaagcatgg acttggggag aatatctatt tgttaaccat
    12121 ggattgacag ggatacgagg ggaagccaaa agaggttatc cttgtgtttt tgaccgagga
    12181 ttgccttatt atcaagcata tcaagggtcg caacaagata agatgataga cactttactt
    12241 tttctatcaa tggaagtaga agataccaat ttgatcaaac gaagtaataa tagacatgtt
    12301 tttgaagagt atcaattact tattcgtcct tattttgaat tagggggtgt aaaaaccata
    12361 caaggtaaaa aatatttgga ttacctaaat cagcaattta aaaagaaaaa ttggagtatt
    12421 ggtggaagtg cagacttatt gatccttaca atctttttag ataaaatcat ggacaaagga
    12481 tggttatgtt gatactataa tacattattt tgtattagta agctaataac taaaaaatgg
    12541 ttcaatacaa atattttata aaaaatggta gtgcctatcc tttcagattg tagacaaaga
    12601 aaaatttctt tgtctacaat ttttttgtta atgacttcag tcagtttcgt gcgcaaagcg
    12661 tgcgaaacaa gaaaaccatg cgtttctaca cgtggaagaa ctagcatatc gaaaggagtt
    12721 ttttcatgtc aaatgacgat aaaagtttag cccatactcg ttggaatttt aagtatcata
    12781 ttgtatttac accaaaatat cgacgaaaag taatctatag gcaattacga aaagatattg
    12841 gaaaaatact taggaagttg tgcaaaataa aaaacgtgga aattatagaa gcacatgcaa
    12901 cgccagatca tattcacatg cttgttcaag taccatcaaa aataagtata tccagtttta
    12961 tagttatctg aaagatcgta gtgctgtgct aatccacgag cagcacgtaa atcacaaata
    13021 tagatacgga aataaatcat tttggtcgga ggaatattat gtgagtacag tcgaattgaa
    13081 tcaaaagaca attgaaaaat acatccgtga acaagaagct gatgatcgca tttgagatag
    13141 tataagtaaa agagagtaga tgttgcaata acaaaagggt aaaaatggac tacctccagt
    13201 aaaataccga aaaatagttt aacttttgga gataagtcca tttaagggtc tatagaatga
    13261 caattttttt aggggatttt actattatta cttcaagacc cgcttggatt cgcaaagtga
    13321 gtgaactctt aaaactacct gatagtaggt tgttttaaga gttatctgaa agtaaaaatt
    13381 gcatttatat ttttgataaa tacgtaaatt tatatatacg tatttatcaa agttttaggc
    13441 caaaggaata ttgtgtaaat acactaggat tgaatgaata gaccagaaga aattgcgtcc
    13501 aagaacaaaa gaagcatgat agtgctctat ataaattggg cattcaagaa tctgaaattt
    13561 aaaggtagaa ttttagttag acttttagat tttaaacaca aaaataacca tacaagcaac
    13621 aagaagcaaa gtgctatatt ataaattgtc cgtccgttta cttacataag atcattgggg
    13681 ctatttttaa aagagaataa tagataataa atgaaaaaaa agtcaagtcc acaatcctgt
    13741 gtaaaatact atacaatgct ctcactattt tttacttggc aaatttaata tattttcttg
    13801 atgaagaaag ccactcatcg tgttgatcaa taa
//
LOCUS       DACEDM010000077        13758 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEDM010000077 DACEDM010000000
VERSION     DACEDM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.077"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(44..1183)
                     /locus_tag="IWO50_002321"
     CDS             complement(44..1183)
                     /locus_tag="IWO50_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0359417.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(1297..1881)
                     /locus_tag="IWO50_002322"
     CDS             complement(1297..1881)
                     /locus_tag="IWO50_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0359418.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(2008..2439)
                     /locus_tag="IWO50_002323"
     CDS             complement(2008..2439)
                     /locus_tag="IWO50_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0359419.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(2457..2777)
                     /locus_tag="IWO50_002324"
     CDS             complement(2457..2777)
                     /locus_tag="IWO50_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0359420.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3509..3766
                     /locus_tag="IWO50_002325"
     CDS             3509..3766
                     /locus_tag="IWO50_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAQ0359421.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(3737..4045)
                     /locus_tag="IWO50_002326"
     CDS             complement(3737..4045)
                     /locus_tag="IWO50_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359422.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            4123..4896
                     /locus_tag="IWO50_002327"
     CDS             4123..4896
                     /locus_tag="IWO50_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0359423.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIRK
                     HGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSST
                     DSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPKA
                     DGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            4911..5114
                     /locus_tag="IWO50_002328"
     CDS             4911..5114
                     /locus_tag="IWO50_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0359424.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            5130..5450
                     /locus_tag="IWO50_002329"
     CDS             5130..5450
                     /locus_tag="IWO50_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0359425.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            5692..6009
                     /locus_tag="IWO50_002330"
     CDS             5692..6009
                     /locus_tag="IWO50_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359426.1"
                     /translation="MIIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            5942..6421
                     /locus_tag="IWO50_002331"
     CDS             5942..6421
                     /locus_tag="IWO50_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAQ0359427.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKEFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            6434..7144
                     /locus_tag="IWO50_002332"
     CDS             6434..7144
                     /locus_tag="IWO50_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010721532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0359428.1"
                     /translation="MEIKSATDILRDKDWRIMIYAKPGTGKTSLVQFLKGKTLVLDMD
                     DSSKVLAGLPNVDVISFDRVKPHEFITEFIKEAPELVTNYDNLVIDNISSFEKDWFVE
                     RGRSSKNGISNEIQDYSQWTNYFARIMTTIYLLKDINILVTAWENQREITTERGQVFN
                     QYAPQIRDSVRDNLMGLTDIVGRMIINPDTGNRGIILEGNDAVYAKNRLDERKATPAE
                     ELFFIGGEPNVSTPTVSD"
     gene            7116..8639
                     /locus_tag="IWO50_002333"
     CDS             7116..8639
                     /locus_tag="IWO50_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0359429.1"
                     /translation="MYQLRPYQIKLVQEARKHLSQGKKGVLIQSPPGSGKSVVIAEIV
                     RLATRKGGTVLFLAHRRELLDNIRETLEQNEVDLSKVIILSAVMAKNRLNKLPKLSLI
                     ITDEGHHGKAKTYMDVYNHFKEIPRLGFTATPYRLNGEGFTDIYEEMVEGPSIQWLID
                     HHNLAPYRWYSIPLIDRSKVDFKNMSREAESSARLFESDATIQGDIVENYKKYADGQQ
                     AIVYAPTIQVSKMIVRWFNDNGIYAVHADGKTPTKERDDIMANFKSKKITILSNVDLI
                     SEGFNVPDVGVIILCRPTQSIVLHLQQSMRGMRYRENKTSIVLDHVGNGANLGLPTDE
                     FEWSLSARKKKSNGSSSEAPRMTCSTCGQQFLLKSLLKIENKPHCPFCLQEIVIKEKE
                     NSVTFDEAVQMVELNAENAKLARLSRKKFSKKQSLELNYAIAKAKVSFEGKGNPLFKM
                     FGSLTAYQGQHYSIDQLEELSLIKDVSMESILRAYKWALEKLNSKQEEPEWVKNTFY"
     gene            8657..9163
                     /locus_tag="IWO50_002334"
     CDS             8657..9163
                     /locus_tag="IWO50_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAQ0359430.1"
                     /translation="MTAFKVDYNEAQDFGAVPDGDYEVVIFNVTEDASKGGTEFINFD
                     MVIRNDIKQPRQNSHLFHRVWKSKETGKYNRGMIMSLAKSFGLPDGKEYQSFENFLED
                     FAMRPAKVKVKNEQSEYNGKTYDNTNIKKFETTKFPELQHQWKKSNAEKSVNSSPAFD
                     ISDDELPF"
     gene            9163..11499
                     /locus_tag="IWO50_002335"
     CDS             9163..11499
                     /locus_tag="IWO50_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0359431.1"
                     /translation="MNNYERIPLELRELNQWGIYKRSWNEQRGKWSKKPYDPFTGELG
                     SSTNESKWSDFKTALSAVSTFNADGLAFYFKPPYIGIDLDDIGDDLERYLQGDVENNL
                     VYVFMNSTKTYSEISMSGKGIHIIGKAAIPGERRRKGNVEMYTEGRFFAITGNFFGNN
                     EEINEIPEIQMNFLYKRYLENETVIKQDFSKSNWSDGNDLSVNEIIQTAVNSSTGNRF
                     RLFMDGGWEKIYDSQSEADLAFANDLAFWTAGDFQKMDEIFRMSSLFRDKYDQKRGKT
                     TYGIGLLNKAISENTNHYTGKKTADDYFLSIPGITVDETKPTKFYSYDDTGNAERYLD
                     LFGSFTKYSYVNKCWYFYNGKNWEQDNIGAVRKWVDQTIEIFKNEPVSIPKDATEDEE
                     KAYIEAKEKHLRRSRNNAGKEAMTRELKHQVAILPDEFDSDDMLFNAQNGYLDLSNGI
                     LYEHDISKMFTRISNAEYTDKSDCPRWKLFLEQIFDNNTELIRYIQKAVGYSMSASIR
                     EQVMFILFGNGRNGKSVFLDIISEIMGSYAMGMQASSLMVKQGGSSGHNEDIARLNGA
                     RLVTSSEPNEGVRMDEGLIKQLTGGDKVSASYKGGHMFDYKPKYKIWLATNHKPIIRG
                     NDDGIWRRLPLIPFTVQIPLDKVDKNLKEKLMRELPGIFNWAVEGCLMWQREGLNPPA
                     DIQKATMEYRKEMDIIGAFIDECCETGPGYSIGATDLFKAYDKWARDMNEHPFSQTQF
                     GKKAADRFEKSKSKGKVVYRGIDLKKEFREFNVLVPGL"
     gene            11814..12131
                     /locus_tag="IWO50_002336"
     CDS             11814..12131
                     /locus_tag="IWO50_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAQ0359432.1"
                     /translation="MTAEIEIQNAIRRELPKYGHFVYRGNVGKVKTIDGRWFDTGLPK
                     GWPDLFGWTKEGKFFAIEVKNEKGKLRPDQVKFGEFLQKQPVLYGVARSVEEALKIVE
                     ESS"
     gene            12128..12289
                     /locus_tag="IWO50_002337"
     CDS             12128..12289
                     /locus_tag="IWO50_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0359433.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            12286..12591
                     /locus_tag="IWO50_002338"
     CDS             12286..12591
                     /locus_tag="IWO50_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359434.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKTK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            12591..12947
                     /locus_tag="IWO50_002339"
     CDS             12591..12947
                     /locus_tag="IWO50_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0359435.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            12907..13152
                     /locus_tag="IWO50_002340"
     CDS             12907..13152
                     /locus_tag="IWO50_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359436.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13149..13562
                     /locus_tag="IWO50_002341"
     CDS             13149..13562
                     /locus_tag="IWO50_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359437.1"
                     /translation="MIPKFRAWVKPDILSNHLNGVIAEALPNFIYGNCLVLREDLEED
                     KQFEEIIDFLDIELMQSTGLKDKNGVEIFEGDIVKTLDADLKVTFSTIKFEEGAFCVD
                     YKDLGTEFDFLYFVDSPLEVIGNIYENSELLEEQR"
     gene            13559..>13758
                     /locus_tag="IWO50_002342"
     CDS             13559..>13758
                     /locus_tag="IWO50_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0359438.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDN"
BASE COUNT         4860 a         2123 c         2685 g         4090 t
ORIGIN      
        1 ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc aaactagtta tcatttttat
       61 ataaactatc gaacacagta ttgatttttt ctttatcctg atcttcaagc tctcgaacaa
      121 tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc
      181 taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt agtccatgca
      241 aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat ttgctgacgg
      301 cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaaattatga gggtttttaa
      361 cttcaaaatt ctcgaataat tctttttgat tgattttaaa cttttttaag agatcaatcg
      421 tatgtttatc aatggaaatt ttcctatttg aagtttcgtt ttttgttttt ccccattcat
      481 ttaatttata attccatgtt ttattaatag tgatgatttg atcttcgaaa tcgatatctt
      541 cccatgttaa tcctagaagt tcagcaaatc gcattccagt agcaccagca actaacacga
      601 gcataggaga agagtactgc gcagaaaggt tttcttctac gagtttcatt aaagatttaa
      661 attgatcata atctaaatat ttatcttctt ttggcttcaa tgaatctttt cctttgatta
      721 ctgcttttct agttggatca aacggaatta atccttcttc tacggcatct ttcaacgatg
      781 ctctaatatg gttattaaac ttaataacgg tggattttac atggtctttc gcatacttat
      841 ttaaaaagcg ttgatatcca actctatcta aatcagaaat caataccgct ggcatatatt
      901 tctttatgtt cattaacgta tcttcgtatt ttctataagt gataggagat actgttcctt
      961 ctttataaag ttgcatccaa tcctcaaagt aatctgataa taatagattt ttgcgatccc
     1021 ctttaaggcc tttgttcaag ttatattcta attcgttggc ggcatctttt gcttcgcctt
     1081 ttgtcttaaa tcctgatttt cttatcttcg tgtattttcc gtcatctttt ttgtatgaaa
     1141 tttcgtattg ccaactattt ccacgtttga ctaatcttgc cataattgaa tctactctct
     1201 ttctctgata caataggcac tataaagaag cctactgtat aggtttgttt ttcttagaac
     1261 acgcttgctt tggacggtgg gcgtgttttt tatttattat acagttgctt taaaaatagc
     1321 tgctttttca aatgatacta aaggagagaa gtggatttct atttctccct tttggtttaa
     1381 cgtgaaatga gcagttacat ccatttgttt tcctggagca acagatccca ttgtattttc
     1441 gttggcatat gtctctgatt ttttgtcatc tggtccatat acttccacat ctgtacctac
     1501 agggatatct gaatcgccat cattttttac ggtataagta atttttacta cttgtgcagg
     1561 ttggttttct tcaaattgat ttctttcatc agttagttct acactattta gtgtatattc
     1621 agcatcacca acttttacag tgtcaccaat cttatagaag gtatcgcttt tttcttcttt
     1681 agaagatgaa gcagaggttg aagatttagt tactttttcg ccaccattat cacttgcttt
     1741 attactatct gatccaccat ttaaagcaga accaataatt ataattaaaa ttactgctaa
     1801 tacccaaaac catacttttt tgtaaaaagg tttactaact ttatacattt ttccgtcttg
     1861 acccataact ttttttgcca tttaaatatt cctcacttct tgttataata tatttgcgat
     1921 ctcagaaatg aggtatgagt ccgtgtgcca gcacgggctt ttttaatgtt ttgctgaaat
     1981 cggttttttt aataattctt gatgtattca tacatattgc cttgcgtaag aatatttttt
     2041 cttaaaatag cattggcaga cagcataaca agtgtatctg agcttattct gaatagaata
     2101 atattccata aatttttcga ggttaaactg agattcatca gtcagttcgt tctcaatata
     2161 gatatttaat aaaattagaa tagctatttt atctgcttct gtttcaaatt ttgagtgaaa
     2221 agttgtagaa gtatcgtaca aaactgaaaa ttcaaaatga gaggcgctga aatgtgcaag
     2281 ttcatgagat aaatgaaagg cttctgcagt ttcgccatat agattttcat tcaaaaaaat
     2341 gattctaggc ttcggataat agaagccagg ctctttcatt tccatgtaaa ctactttcaa
     2401 attgtattcg ctcaacattt ctttcaattt caaatacata caacccatca ctccaactat
     2461 tcattttcct ctaaagcttt agcaattgca atcgctttac gcattgtctc cttagatatt
     2521 tcttttccat caaaagaaaa aacagtatcg ttttctgata aatccacatg tttaggggtt
     2581 tctttttctt cgcgccccag aagatagtct acagagacat cgaaatagtc agcaatttct
     2641 tgcaaacgtt ctgtagaggg gtttgagttc ttaagtctat ataagacgtt tttaccatag
     2701 cctaggtctt cttcaacttt atttaatgat tttccctgtt ttttcgctaa ctcttttatt
     2761 ctctcaaacg ttatcatatc aatattcctt tcgttcaaga aaaaatattt aactttattt
     2821 gttaaaattc gttgactatt ttaacaaaag gtgttaatat cattcttgta aacaagttta
     2881 acaactaaaa agacaacaaa aaacactatt gattaataaa tgccaaccgc caagaaagct
     2941 tttaaaatca atgtttatat gtcttattta actatgttct gattttaaca ctgtatgtta
     3001 atttagtcaa cgcagttttt taaaaagttg ttaaatttgt ttacgaatat aaaagaaagg
     3061 agagaagaat atggaaaaaa cagtctcggc aaaaatcgaa gatttgaaat tagacatact
     3121 gaaacaagca aaagtggcga tggaacacgc ggtagataaa gaagactctg ccatggttgc
     3181 agccatagca gagattttag ctcacgttta gtcatcgttt ttacggtctt ctggaagctg
     3241 accatagata acagcataat gaatatctaa ataagcttga acaatttctt ttggcgaaat
     3301 tgattcgcct ttagtagata cttgtgattc gtgatttagc catgcaacaa ctaaatccgc
     3361 agcgatttca aacggcgtta cactttgtgt cagttgccat aaagaatttc acaatatgta
     3421 tggtcgtggt aataatacca aagagcaatt tgaggaattt atggaaatta aaaaatacaa
     3481 aaaatcattt attaaaggag gctaaacaat gaacaaggaa atgagccaaa caatcaaggt
     3541 acaaaaaatg atagatgatc taacacatgg aattgatagc caagccgata aaatcatcaa
     3601 ggaattgcaa ggacaaaaag ttaaggatgc aaaaatgctt ctcaagacta tcaactttga
     3661 aatgaatcca acaaaaagaa agctcgccga tgtgttggaa gaaaagttgg cttccgctat
     3721 caacgagcaa gaattactat ttgaaaccga tacttttaac agctgatgtt ttatagatga
     3781 cagtttcatc cgaatctata tcatcgccga gagaaaagaa tgttgctttt gacaaaaagc
     3841 cgataagacc tacaagcggt cttgatgttg caagagtaga taccccaata tcaccttgca
     3901 aggtattcac gtaaatttga gaagaataga acattccatc tttttcttcg atatttgacg
     3961 catcatcgta tccaacgacc aaagtatcgg caaatatttt gattttttct ccatcaagca
     4021 tagtaattac aaattctggt ttcataaatt ttcacctcct taacaattat ttcagcctgt
     4081 cacactgata aggaaattat accaaagaaa ggaatgagaa atatgaatac accacaaatt
     4141 tttaatttcg agcaaaacga agttcgaact attttagtaa atgatgaacc atattttgta
     4201 ggaaaagatg tagcagacgt tctaggatat tcgaatccgc aaaaagctat tcgagatcat
     4261 gtggacttgg aggataagac gcagaacgat tcgttcaccg tcaatggaac agcagttgtt
     4321 ttgattaacg agtcaggcct ttacagttta atcttaaaat caaaacttcc ctctgccaaa
     4381 aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa ttagaaaaca tggaggttat
     4441 ctaactccag aaaaagtaga agaagctttg cttaatccag atacaatcat tcaattagca
     4501 actcaactaa aagaagaaag aactggaaga ttaatcgcag aacaaaagat tgcagagtac
     4561 gaacctaaaa tctcctattt agatagcata ttatcttcta cagattcagt aacaattagt
     4621 cagattgcag cagattatgg gatgtctcca caacagatga ataaattact tcataaacta
     4681 ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca aaaaacacat gaaccaagga
     4741 tacacaaaat ctcatacaac tgagatcccg aaagccgatg gtggcactaa aattgtaatg
     4801 aataccaaat ggacacagaa agggcgtcta tttatctacg aattactaaa aaaagaagga
     4861 tattaccctc aaatggattt agaggaaatt ggttagaaag gagttttagt atgactgaca
     4921 ttgcagaaat cactcaacga gatagagaaa aaatcaaaga atatgtcgaa agttcaaagt
     4981 tcttaactta caccatgctc gctgaaagat tcggcattag caaaagctac ttatctttaa
     5041 ttttaaacgg taaaaagact tctgcagaag caaatagaat catagattcg attattacta
     5101 tgtacgaatt gtaaagggag gaacagctaa tgcaatatct agaagcgaaa atcccagttc
     5161 cagaaggcta tgtaattatc tcccaagtgg attatgagga gttaaaaaaa gctgatgata
     5221 ctggtagatg gatgacgttg ccagaagtgc tagaacggat taacagaaaa tatgattggt
     5281 ttacttctag agttttaaag aacccaagat atagaaatat tatcgatatc gaaaaaaaca
     5341 aaaatggatt tgtctattat ccagttgaag gaagagacac atatctattt ttaagaagta
     5401 aaacacttga atttttagaa acaaattttt cggaaatctt aaggaggtaa gcggatgggt
     5461 aaatttaata gagcactagt attcagcgca ccactaatta tctacgcttt aggtctttgg
     5521 ggaagcagac aagcattaat cggcacaatt gtatatatgg tctggatctt tatggggctt
     5581 gatgaagctg agtacagagc gaaaaaaaga cacgaacgcc ggcaagcatg atcgtgtcca
     5641 aagaaataag ctatctttct atattttacc acagaaggga atgaaccgta aatgataatt
     5701 gcaacggata cattagatcg aatcttttta aaagacgaat acaaactgcg caatatagat
     5761 gcgtcgggaa ttttagtatt tgatctttat gacaatggaa aaattggcat ctatcaagca
     5821 agtgatattg aagaaacaaa ccttgctttc gagcaaattg atgattctgt ggaattggat
     5881 ttagatgaag caatcctagc ttttgaacaa attgcaaaat tattaaagga ggcacaaaag
     5941 aatggcaact ctttaccaac tcagcgagtc atatatcaaa gtcctggaac tggcagaaca
     6001 attggatgaa gaaattcttc gcgatactct cgattcgatt aatgaagcga tcgagtataa
     6061 ggcagaaaac ttagcaaaaa tagttaaaga agtagaaggg aaagctgagt taatcgatag
     6121 tgaaattaaa cgtctacagg agcgtaagac atcgcttttg aacaatgcta agagtatcaa
     6181 acactattta caagaggaga tggaaaagac tggtaaaacg aagattaaag gtgaattatt
     6241 caacattgga attcaaaata atccagtatc tgtcaatgta atcaatgaaa acttaattcc
     6301 aaaagaattt tttacccctg tgcctccaaa attggataaa aagcaattga aggaggaact
     6361 gaagcacgga gatattcctg gtgctgaact cgtccaaaca aaaggtttga ggataagata
     6421 ggaggcaatg ttcgtggaaa ttaaaagcgc aacagatatt cttcgtgata aagactggag
     6481 aataatgatt tatgcaaaac caggtacagg taagacgtca cttgttcaat ttttaaaagg
     6541 aaaaacttta gtgttggata tggatgattc ttcaaaagta ttagcaggtt tgcctaatgt
     6601 ggatgtgatc tcgtttgatc gtgttaaacc acatgaattc attactgaat ttattaaaga
     6661 ggctcctgaa ctagttacaa attatgacaa tcttgttatc gataatattt caagttttga
     6721 aaaagattgg tttgtagaac gtggaagatc ttctaaaaat ggaattagta atgaaattca
     6781 agattactca caatggacga actattttgc tcgaattatg actaccattt atctattaaa
     6841 agacatcaat attttagtaa ctgcatggga aaatcagcgt gaaataacta ctgaacgcgg
     6901 acaagtattt aatcagtacg caccacaaat cagagatagt gtcagagata atcttatggg
     6961 tctaactgat atcgttgggc gaatgattat caatccagat actggcaatc gagggattat
     7021 cttagaaggt aatgatgctg tttatgctaa gaacagacta gatgaacgga aagctacacc
     7081 tgcagaagaa ttgttcttca taggcggtga accgaatgta tcaactccga ccgtatcaga
     7141 ttaagctagt tcaagaagcc agaaaacatc tatctcaagg aaaaaaggga gtattgatcc
     7201 aatcgccacc aggaagtggt aaatcagttg taattgcaga aattgttcga ttggcaacaa
     7261 gaaaaggtgg cacagtacta tttcttgccc acaggagaga gttacttgat aacattcgag
     7321 aaactcttga acaaaatgaa gtagatttat caaaagtcat aatattgtca gctgtaatgg
     7381 ctaagaacag gttaaataaa ttgccaaagc tgtctctgat aattacggat gaagggcatc
     7441 acggtaaggc gaaaacttat atggatgtct ataaccattt taaagaaatt cctagattag
     7501 gttttactgc tacaccatac agactcaacg gggaaggatt tacagatata tacgaagaaa
     7561 tggtagaagg accatccata cagtggttga ttgatcatca caatctagcg ccttatcgtt
     7621 ggtattctat tcctttgatt gatcgttcta aagtagattt taaaaatatg tcacgtgaag
     7681 ctgaaagttc tgcgcgatta ttcgagtcag atgccacaat tcaaggcgac attgttgaaa
     7741 attataaaaa atatgcggat ggtcaacaag cgattgttta tgctccaacg attcaggtaa
     7801 gcaagatgat tgttagatgg tttaacgaca acggaatata tgcggttcat gcagatggga
     7861 aaacacctac caaagaacgt gatgatatta tggcaaattt taaatcgaaa aaaatcacta
     7921 tattatcaaa tgtcgattta atcagcgaag gatttaatgt accagatgtt ggagtgatta
     7981 tcctttgtag gcctacgcaa tcaatcgtct tacatttaca acagtctatg cgaggcatga
     8041 gatatcgaga aaataagact tcgattgtgt tagatcatgt tgggaacgga gctaacttag
     8101 gtttacctac tgatgaattt gaatggtcgt taagcgcgag gaaaaagaag agtaatggaa
     8161 gtagcagcga agcgcctaga atgacttgct caacatgtgg acagcagttt cttctgaaga
     8221 gcttattaaa gatagaaaat aaaccacatt gcccgttctg cttacaagaa atagtaatta
     8281 aagaaaaaga aaattccgtt acttttgatg aagcggttca aatggtggaa ttgaatgcag
     8341 aaaatgccaa gctagcgcga ctttcaagaa agaaattttc aaaaaaacaa tctttagaat
     8401 taaattatgc gattgcaaaa gcaaaggtaa gttttgaagg aaaaggaaat ccgcttttta
     8461 aaatgtttgg ctcactcact gcttatcaag gacaacatta ctccatcgat caattagaag
     8521 aattatcgtt gatcaaagat gtatcaatgg aatcaatttt aagagcttat aaatgggctt
     8581 tggaaaaact caattcaaaa caagaagaac ccgaatgggt aaaaaataca ttttattaag
     8641 gaagaggtaa ttaattatga cagcatttaa agtagattac aatgaagcgc aagattttgg
     8701 agcagtacca gacggagatt atgaggtagt tattttcaac gttacggaag atgcctcaaa
     8761 aggcggcaca gagtttatta attttgatat ggtcatcaga aatgatatta aacaaccacg
     8821 acaaaacagt catcttttcc atagagtttg gaaaagtaaa gaaactggca aatataatcg
     8881 tgggatgatc atgtcactag caaaatcatt tggccttcct gatggaaagg aatatcaatc
     8941 attcgaaaat ttccttgaag attttgctat gagaccagcg aaagtaaaag taaagaacga
     9001 acaatcggag tacaacggga aaacgtatga caatacaaat attaaaaaat tcgaaactac
     9061 taagttccca gaactccaac atcaatggaa gaaaagcaac gctgaaaaat ctgtaaattc
     9121 ctcaccagca tttgatattt cagatgatga acttccattc taatgaataa ttacgagcgt
     9181 attcccttag agttgcgtga gttaaatcag tgggggattt ataaacgctc atggaacgaa
     9241 caacgaggga aatggagtaa gaaaccttat gatccgttta ctggagaatt aggcagcagt
     9301 acaaacgaga gcaagtggtc tgatttcaag accgctctct cagctgtttc aacttttaat
     9361 gcagatggcc tagcttttta ttttaaacca ccttatatag ggattgattt agatgatata
     9421 ggtgatgatt tagaaaggta tcttcaaggc gatgtagaaa ataatttagt ttacgttttt
     9481 atgaattcta caaaaacata ttcagaaata tcaatgagtg gcaagggaat ccatattata
     9541 ggtaaagcag caatccctgg agaaagacgt agaaaaggga atgtcgaaat gtataccgaa
     9601 ggtaggttct ttgctattac aggtaacttc tttggtaata atgaagaaat taatgagata
     9661 cctgaaattc aaatgaattt cttatacaag cgatatttag aaaatgaaac agtaataaaa
     9721 caagattttt ctaaaagtaa ttggtcagat ggaaatgact tatctgtcaa cgagattata
     9781 caaacagcgg tgaattcttc cacaggtaat cgttttagat tattcatgga tggaggatgg
     9841 gaaaaaatat atgatagcca atctgaagca gatttagcat ttgcaaatga tttagctttt
     9901 tggactgctg gagattttca aaagatggat gaaatattcc gaatgtcttc gttatttcga
     9961 gacaaatatg atcagaaacg cggaaagaca acctatggaa tagggctttt aaataaagcc
    10021 atatctgaga atacaaatca ttatactggc aaaaaaacag ctgatgatta ttttctttcc
    10081 atcccaggta tcactgtgga cgaaactaaa ccgactaagt tttatagcta tgacgataca
    10141 ggaaatgcag aaagatatct tgatttgttt ggatcattta caaaatacag ttacgtaaat
    10201 aaatgctggt atttttataa cggtaaaaac tgggaacaag acaatatcgg tgccgttaga
    10261 aaatgggtag atcagactat cgagatattt aaaaacgaac ctgtttcgat acctaaagat
    10321 gcgactgaag atgaagaaaa agcctatatt gaagcaaaag agaaacattt aagaagatct
    10381 agaaacaatg ctggaaaaga agctatgaca cgtgagttga aacatcaagt agcgatactt
    10441 cctgatgaat ttgatagtga tgatatgctg ttcaacgctc aaaacggtta tttagatctt
    10501 tctaatggca ttttatacga acacgatatt tctaaaatgt tcactcgaat ttctaacgct
    10561 gagtatacgg ataaaagtga ttgtccacgt tggaagctgt ttttggaaca gatatttgac
    10621 aataacactg aattgattcg ctatattcaa aaagccgttg ggtattctat gagcgcatca
    10681 ataagagaac aagtcatgtt catccttttc ggtaatggaa gaaatggtaa gtctgttttc
    10741 ttagatatca tttctgaaat aatgggaagt tatgccatgg gaatgcaggc atcaagcttg
    10801 atggttaaac aaggtggtag cagcggtcat aacgaagata ttgcacgatt aaatggcgca
    10861 cgtctagtaa cgtcctcgga gccaaatgaa ggcgtaagaa tggatgaagg tttaatcaaa
    10921 caactaactg gtggagataa agtgtcagca tcctataaag gaggccacat gtttgactat
    10981 aaacctaaat ataagatttg gcttgcaact aatcataagc caatcatcag aggaaatgat
    11041 gatggtattt ggcgaagatt gccattaatt cctttcactg tacaaattcc attggataaa
    11101 gtggataaga atttgaaaga aaaattgatg cgcgaattgc caggaatatt caattgggca
    11161 gtagaaggat gtttgatgtg gcaaagagaa gggttgaatc caccggccga tattcagaaa
    11221 gctacaatgg aatatcgaaa agaaatggat attataggtg cttttataga tgaatgttgc
    11281 gaaacaggac ctggttattc aatcggggca acggatctat ttaaagcata cgataaatgg
    11341 gcaagagata tgaatgaaca tccattcagt cagacccagt ttggtaagaa agctgcggac
    11401 aggtttgaga aatcaaaatc aaaagggaaa gttgtttatc gggggattga tttaaaaaaa
    11461 gagtttagag aatttaatgt attagttcct gggttgtgaa acaaaaaagt ttcacaaatg
    11521 tgtgtaggta gacagttggg tagatagtaa gtagatagtt ttttacaaac tgtctacccg
    11581 ataaaacctt tggggctgta aggctcatta ctgttaggta gatagtaggg atagtttata
    11641 tatatagtaa ataaaataat aaaaataagg aaatatataa aaatacagaa gcaacctaga
    11701 aataactgtc tactgtctac ccaaactggc taatacttag agccacaagg gatagagcga
    11761 ttataaactg tctacccatg tatatatact atctacccca gcaggaggcg cttatgacag
    11821 cagaaattga aatacaaaat gctattcgaa gagaattacc aaaatatggt cattttgttt
    11881 atagaggcaa tgtgggcaaa gtgaagacga ttgatggcag atggttcgat actggattgc
    11941 ccaaaggatg gccagattta tttggatgga ctaaagaggg aaaatttttc gccattgaag
    12001 tgaagaacga aaaaggaaag ttgagaccag accaagtgaa gtttggtgaa tttttgcaaa
    12061 agcaacctgt actatatggt gttgcacgat cagtggaaga agcattgaaa attgtggagg
    12121 aatcatcatg acaacagaag aagtgattca aatgcgaatt cgaagcattc aacgtgagat
    12181 tgacgatctg gaacgaacaa aggcagtgat ggtcaatgaa acggcgagaa aggcaatcga
    12241 tttgcacata gagaacttaa gaagggaaat tcgtagattg gaggaatgag cgtggataag
    12301 aaagcagcaa tgaaacgaat catcgaactg acacattctg agaattggca agaagacaaa
    12361 gaaatagttg cagaagttca aaagctcggt aaatcaatgt gggctgaaaa aaccaaacgg
    12421 aaaacgccga gaaaaattgc aatctggcat ggtgatcgaa ttctagtaac aggtaccgct
    12481 gaacagttat ctgaaattac tggtctgagc aaaaacatca tctgggatag agctaggagc
    12541 ttatggattg attcaaaagg acgacagttt aggtatgtgg aggagagata atggatctca
    12601 ttacacaata cagtgacatc atcctcaaga aaatcatgat gaagattcag aaagataaaa
    12661 aatcaaaaga acgagctgaa ttagttaagt tagaaatggc tgaaacagga gcaggagtgc
    12721 gaagtagcag gcattggaaa gcagcagcaa acattgaatt ttattacaac gaaattcaaa
    12781 aagggttcga tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag aaacttcatc
    12841 aagatcgttt caaatttgta gagaagtata aagaaatatt agaagagtat ttgaggagga
    12901 cagcaaatga taaaaaaact cgttcaattc agcatggatt tatatgatat cgaatcagga
    12961 gcaacactat ctgtggaatc ggaccatcta atcataaatt ttggtggaaa gcgccagatt
    13021 attttgtggg tagttgatga tgtactgttt ccagaaattg ttcatgattt cgaagaatca
    13081 aaagcggttg agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag
    13141 gacagcgaat gataccgaag tttagagcgt gggtaaaacc tgatatttta agcaatcatc
    13201 ttaatggggt gattgctgag gcattaccta attttattta tggcaattgt ttagttttga
    13261 gggaagattt agaagaagat aagcagtttg aagaaattat tgattttctt gatattgaac
    13321 tcatgcagtc cacaggtctg aaagataaaa atggtgtgga gatatttgaa ggagatattg
    13381 tcaaaactct cgatgctgat ttgaaagtaa ctttttcaac gataaagttt gaagaaggcg
    13441 cattttgtgt tgattacaaa gatctcggca cggaatttga ttttttgtac tttgtagatt
    13501 cacctttgga agtcatcggg aatatatacg agaatagcga gttattggag gaacagcgat
    13561 gaataaacag gaattgattg ataaactatc aagccttgta ggtagcatag aggactttaa
    13621 aggtataagt acttttcatg ctggaaaata tgcgggttta gaacacgcat tagaactaat
    13681 ccaacaacta aatgaatcgc agaaaccagt tattccacaa ctcgtggcag gttggatcga
    13741 aaaaagtacc gacaattt
//
LOCUS       DACEDM010000078        13747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEDM010000078 DACEDM010000000
VERSION     DACEDM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.078"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..863
                     /locus_tag="IWO50_002343"
     CDS             <1..863
                     /locus_tag="IWO50_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0359439.1"
                     /translation="MSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITS
                     GKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEG
                     QILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFV
                     QANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTET
                     EGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYA
                     DMYKLQSSGI"
     gene            1189..2040
                     /locus_tag="IWO50_002344"
     CDS             1189..2040
                     /locus_tag="IWO50_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0359440.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2137..4017
                     /locus_tag="IWO50_002345"
     CDS             2137..4017
                     /locus_tag="IWO50_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0359441.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4057..4959)
                     /locus_tag="IWO50_002346"
     CDS             complement(4057..4959)
                     /locus_tag="IWO50_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0359442.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5190..6593
                     /locus_tag="IWO50_002347"
     CDS             5190..6593
                     /locus_tag="IWO50_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0359443.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6593..8026
                     /gene="ascB"
                     /locus_tag="IWO50_002348"
     CDS             6593..8026
                     /gene="ascB"
                     /locus_tag="IWO50_002348"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0359444.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8171..9097
                     /gene="trxB"
                     /locus_tag="IWO50_002349"
     CDS             8171..9097
                     /gene="trxB"
                     /locus_tag="IWO50_002349"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0359445.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9311..11038
                     /locus_tag="IWO50_002350"
     CDS             9311..11038
                     /locus_tag="IWO50_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0359446.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11110..11403
                     /locus_tag="IWO50_002351"
     CDS             11110..11403
                     /locus_tag="IWO50_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0359447.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(11495..12355)
                     /locus_tag="IWO50_002352"
     CDS             complement(11495..12355)
                     /locus_tag="IWO50_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0359448.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            12447..12875
                     /locus_tag="IWO50_002353"
     CDS             12447..12875
                     /locus_tag="IWO50_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0359449.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13037..13213
                     /locus_tag="IWO50_002354"
     CDS             13037..13213
                     /locus_tag="IWO50_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0359450.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            13240..13686
                     /locus_tag="IWO50_002355"
     CDS             13240..13686
                     /locus_tag="IWO50_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0359451.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4498 a         2349 c         3074 g         3826 t
ORIGIN      
        1 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
       61 gtatcttgaa gattatggat acagaagaat atgcaccaca acaaaaccct ggagcagatc
      121 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
      181 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
      241 gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt tacgaatttt
      301 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
      361 gtaagaaaat ggggatggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
      421 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
      481 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
      541 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
      601 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
      661 gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt gcgattgccc
      721 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
      781 tagaacacgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
      841 aactgcaaag cagtggtata taaaagatgt tgtgacagta gcattttgac ctaagcacta
      901 gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt gtcgtagtta
      961 ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc taagaaagcg
     1021 ttgacaaaat cgtttgcttt cgataataca aagaaaacga tacaacttgg atagtgattg
     1081 cctaatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     1141 tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat gctgattgag
     1201 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     1261 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     1321 gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga attggcacaa
     1381 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggat
     1441 ctacaagtcc atttgaatga ttctgtgtat attgcattga cggatcattt gtatacagct
     1501 atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga tatcaaacaa
     1561 ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc ggataaatac
     1621 cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt caatgcacaa
     1681 ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca ggatatttta
     1741 aatatcgtcc gctattacta taagattaca tttgatgaag attcaatcca tttttttcga
     1801 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagagaa atcagaagtg
     1861 ggagaagttt ctgaggaatt gttccaggtt attcaaaaaa aatataagga cacgtttagt
     1921 tgcattgaga aaatagcaga ctttttaacc ggtgctttcg actaccagat gtccacggat
     1981 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     2041 ataaaaaagg aggacaaatt aacaagaacg tatgaaaata agggtagtga cattcagttg
     2101 gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca ggaattagca
     2161 gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg
     2221 acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag
     2281 atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt aatcgggaat
     2341 catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga tgaagcagaa
     2401 gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt aagtggctgt
     2461 ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt aaatgctttg
     2521 ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct gaatgggata
     2581 ggggatgcaa ttttttattt catgccagtg atcttagggt acacagcagc gaaaaaattt
     2641 catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa
     2701 ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag cttgctgggt
     2761 cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta taccagtagc
     2821 gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat tgccaagaaa
     2881 tggattcctg cagtcgtcca gacctttttg gttccttttt ctgtgttatt aatcgcactt
     2941 cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct attaagcacg
     3001 ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct agggtttctt
     3061 tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc gattatgcag
     3121 ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag ttttgcacag
     3181 acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc
     3241 atgccagctg tgatttcagg aatcttcggc gtaacagaaa cggctattta tggaatcacc
     3301 ttaccaaaga agaagccatt tatttactcg atgattggtg gtgctgtttc aggaatctat
     3361 ttgatggcat ctggaacaat cagctacaca atgggcgggt tagggatttt tggggtggta
     3421 aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg cattgctatt
     3481 gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc aggtgaaaca
     3541 gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
     3601 gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
     3661 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
     3721 ttcccgacga aacatgcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat
     3781 gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga
     3841 gaaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa
     3901 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
     3961 gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
     4021 gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa
     4081 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac
     4141 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     4201 gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg
     4261 ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat
     4321 actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat
     4381 cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta
     4441 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     4501 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     4561 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     4621 aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     4681 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     4741 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     4801 tgctacacgg atgagtgtag acgggtggac atagcatcgc tcagcgatct cttggatggt
     4861 catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg
     4921 agaaaaggag atttctttga ttttttcacg taataacatg tagttacctt cttcttattt
     4981 ttttacgtaa attataactt ttttgaaaat gttttgtaaa cggatttata ctatataact
     5041 taaaatagac tttttttgac aagatattta caaaaatcca ctagaaaaca gtaacttttg
     5101 taaatgttac tggaaagaaa cggatttcta ttgtttgttt tgtttacaaa aaagataatg
     5161 aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg
     5221 gtggaggcag cagttacacc cctgagttga tggaaggatt tatcaaaaga tatgaggaat
     5281 taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg
     5341 ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa atctatgcaa
     5401 ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg
     5461 gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc
     5521 aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca gtggtcttgg
     5581 caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc
     5641 caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat
     5701 tatgcaatgt gccagtaatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag
     5761 aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata
     5821 ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gacaatggat
     5881 tgccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaatatga
     5941 tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa catgctttag
     6001 aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat
     6061 ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg
     6121 gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa
     6181 cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg
     6241 tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc
     6301 ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaatta ctgacgatcg
     6361 aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc aatccgctta
     6421 tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt
     6481 atttaccgca attcaaggaa acaatcagcc gattaaaaga agaaggaatc gaagttcaag
     6541 atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt
     6601 cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga
     6661 tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc
     6721 tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa
     6781 aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg
     6841 gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgaaaa
     6901 agagccgaat gaagctggct tgaaatttta tgaggatgtc tttaaagaat gccaaaagca
     6961 cggaatcgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga
     7021 atacggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat
     7081 cttcaatcga tataaaggac ttgtcaaata ctggctgaca ttcaatgaga tcaatatgat
     7141 ccttcatgca ccattcatgg gagcaggact gtactttgaa gaaggagaaa acgaagaaca
     7201 agtcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc
     7261 tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc
     7321 atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt
     7381 cttcattgat gttcagtcac gaggagaata cccagcttat gcattgaaag aaatggaacg
     7441 aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt
     7501 tgattttatc tctttttctt attattcttc ccgagtttcg actaccgaca acgaactctt
     7561 ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga
     7621 atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg
     7681 ttatcaaaaa ccattattta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga
     7741 gaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat
     7801 gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat
     7861 tgatttagta tcagctggta ccggtgagat gaagaaaaga tacggattca tttatgtcga
     7921 tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta
     7981 taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa
     8041 aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc
     8101 agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga
     8161 gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc
     8221 tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg
     8281 ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc
     8341 cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg
     8401 aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag
     8461 ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc
     8521 tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt
     8581 tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat
     8641 ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc
     8701 acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac
     8761 agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt
     8821 caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga
     8881 tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac
     8941 agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa
     9001 agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta
     9061 taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt agaagaacgg
     9121 gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga
     9181 acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc
     9241 atactttcat atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg
     9301 tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga
     9361 agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc
     9421 tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag gacctgggat
     9481 caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga
     9541 tacacaaggt gaagaagcaa aacgccgagg tgtcgctatt gcttatgact cacgccatca
     9601 gtcaccagaa tttgcaatgg aagcagccaa aacactggca catcacaata tcccgtctta
     9661 tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac
     9721 gtttgcggga atcatggtaa ctgcatctca caacccagcg gcttacaatg gatataaagt
     9781 ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt
     9841 ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag
     9901 cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc
     9961 agtgacgatt gatcgtgaat tggtagaaac aatggggaaa gatttgaaac ttgtgtacac
    10021 gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga
    10081 acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc
    10141 gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg
    10201 agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt
    10261 gccagatggt tcttatgaag tattgactgg gaaccaaatc ggggcattgt ttgctcaata
    10321 tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc
    10381 aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa
    10441 tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caattcgaag aagaccattc
    10501 acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg
    10561 cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa
    10621 acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg gctattttgc
    10681 agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt
    10741 gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac
    10801 agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac
    10861 accgccatcc gacgtattga aatatacatt ggaagaaaat ggctggattg ctatccgccc
    10921 ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagct ctacagaaga
    10981 cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa
    11041 aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag
    11101 gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa
    11161 atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat
    11221 gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt
    11281 ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt
    11341 cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa
    11401 taacgatagg tgcaaataga taaacactgg agtgtgacaa cgaaatttct tcgttgtcac
    11461 actccagtgt tttttaagaa aaagacaaaa atgattaatc gcttttttct gttgtttcag
    11521 ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt
    11581 caatagataa agatgccaca ttgatcacta cacatcctag tttttcataa agatcattcg
    11641 caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc
    11701 cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta ttcgttagtc
    11761 ccacgatttt tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca
    11821 aaggtaaatt ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg
    11881 aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc
    11941 catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca
    12001 ataaatggag ggcaccaggt tgccgaattg gcttgatacc ggttaaacgt tcaatttctg
    12061 atatcggtgg cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgtg
    12121 ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg
    12181 catcttgcaa tgcctgcttt aatgtttttt catctgtcac gaacgttctg cggaataggt
    12241 tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc
    12301 cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata
    12361 aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta
    12421 atcattttga tttggaggaa taagtcatga aaaaacagac attgatcgaa caatcattag
    12481 aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt
    12541 tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat
    12601 tcaaaggggt aagttatgat acgatttatc ggaatctcca tgattttgca gagatcgatt
    12661 tactggaaga aaccgattta aatggagaaa tgaagtttcg ttttcattgc tgtcaggggg
    12721 aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata
    12781 tgtgtccgat gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat
    12841 tcgagatttt cggaagatgt gaaaattgtc aataagggtt taaataaaat aacatttgat
    12901 aattttaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac
    12961 aggacagtct tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg
    13021 gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc
    13081 tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg
    13141 tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg
    13201 caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta
    13261 aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga
    13321 atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa
    13381 gaagaactaa cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt
    13441 gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa
    13501 aaatatatgc cggaacaact ttccgatgaa gaaattcgac aacttgttca agaagcaatc
    13561 actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct
    13621 aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa
    13681 tcttaatttt atcatttaaa ctgcggacag tgattgtccg cagttttttg tgttcgtaaa
    13741 aaaagaa
//
LOCUS       DACEDM010000079        13688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEDM010000079 DACEDM010000000
VERSION     DACEDM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.079"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(38..1603)
                     /locus_tag="IWO50_002356"
     CDS             complement(38..1603)
                     /locus_tag="IWO50_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0359452.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(1596..3047)
                     /locus_tag="IWO50_002357"
     CDS             complement(1596..3047)
                     /locus_tag="IWO50_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0359453.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(3061..4077)
                     /locus_tag="IWO50_002358"
     CDS             complement(3061..4077)
                     /locus_tag="IWO50_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0359454.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            4297..5385
                     /locus_tag="IWO50_002359"
     CDS             4297..5385
                     /locus_tag="IWO50_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0359455.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            5439..6965
                     /locus_tag="IWO50_002360"
     CDS             5439..6965
                     /locus_tag="IWO50_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359456.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            6967..8172
                     /locus_tag="IWO50_002361"
     CDS             6967..8172
                     /locus_tag="IWO50_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0359457.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            8429..9406
                     /gene="guaC"
                     /locus_tag="IWO50_002362"
     CDS             8429..9406
                     /gene="guaC"
                     /locus_tag="IWO50_002362"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0359458.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            9670..11094
                     /locus_tag="IWO50_002363"
     CDS             9670..11094
                     /locus_tag="IWO50_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0359459.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            11555..12664
                     /gene="pdhA"
                     /locus_tag="IWO50_002364"
     CDS             11555..12664
                     /gene="pdhA"
                     /locus_tag="IWO50_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0359460.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            12667..13644
                     /locus_tag="IWO50_002365"
     CDS             12667..13644
                     /locus_tag="IWO50_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0359461.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         4366 a         2347 c         2963 g         4012 t
ORIGIN      
        1 gcaaatggaa agagtatcat tgtacggatt tcgtttatta gttatttttg tttttaagga
       61 aaagctgtgt ccgatattct tttggagaaa tcccttgttt tttcttaaat aaataactga
      121 aatagttagg gtcattataa ccaatttcaa aagcaatatc tgaaatacga tagtctgatt
      181 gctgcaataa tttttttgct aattcgattc tttgttctgt cagataatcg atgaatgttt
      241 ggttaaacga ttgagaaaaa atcgtactga aatgagaggg acttaatgca acttgttgag
      301 cgacccaagt taaggacata tctggatcgg taaaattttc tttgataaaa tccaatgcat
      361 ggttcaaaat tggctgatat ttgttccttg aaggctgtga gggcagagca gttaagaaag
      421 aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt
      481 gactggtgga tgtgcaatga accatttctt gcgggatttt cttttctttc aggcagacat
      541 gctggagtag cttagtcaag acaaataaac gatataatcg tgttcgagtt ggtgtgcctt
      601 gtggtttcgt caagtgttcg atgtacttag atacttcgtt tttcgataat gaagacaaat
      661 caagtgagag gtcaaagtaa aagacagaat caactggaag tttcgcttct tgaatcaaat
      721 cctcatagct gataatttgt tctttaaggg aactggcggt ttcaagtatc atctgcgtga
      781 tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac aggaccaatg gcgacagaaa
      841 aattattttt ttgtcccttt tctaattcat ggaccaacgt ttgggctacc tgataactgg
      901 tctcgatcac attttcttca agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa
      961 cagcagagaa gatcacttgt tgattctccc taaataaaaa cttcaaataa tcactgagat
     1021 gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc taataatacg gtcatttttt
     1081 tccctgctaa agagcgattg aaagattcag caagtttcaa tgtttcactg atcgtaagtg
     1141 agcctttgaa caaaccgttc aagaaatgat tttttttcaa ttcgaaaaca agatcagaag
     1201 aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac tgctttttct attgcttgtt
     1261 tcaattcgct acttttaatc ggtttaagca gataatcatc tgcctgtacc tggattgctc
     1321 ctttcatata atcgaaatcg tcgaatccac taataaatat gagacgagtc caaggaagta
     1381 gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa aggcatttgg atatcagtca
     1441 gaatgatgtc gggctgtaag tcgataattg aagacagtgc catctcacca tctccagctt
     1501 cgccgacata agtgattgga taataagaac caaggacagt tatttgttcc cgcaagttat
     1561 tgcgaatgag gtattcgtct tcgatgataa aaactttata catgtttgtt tcctccaatt
     1621 gttttgggag atagagggat catcagtgtg acagttgttc cttctcggta tttgctgtca
     1681 atcgtgatag gtttttgatc atttccgtaa taaagaagga gacgcttgtt tacgttgtaa
     1741 agtccgtagc cgatactgaa atctgaatcg atccctttgg ctaactcttt acgtatttct
     1801 gccagttttt ctgcagggat gccaatcccg ttgtctataa ctttgaattg gatcatctca
     1861 tcaattttgc atgcggtaat acgaataaat cctttgtgac ggacgtactt cgttccatgg
     1921 tatacagcat tttcaatcaa tggctgcaag atcattttta aaatagaaac aggaaaaagt
     1981 gtttctggta tatcgatctt atagtcaagc atatggccgt atcttatttt taagatgact
     2041 agataatcat tgatatggct aatttctttt tcaagaggaa tccagtcttg tcctttgctt
     2101 aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag
     2161 tcttgttcga tcaaagaaat aatggcatcc aaactgttat aaataaaatg gggagtaatc
     2221 tgagcttgca atacacgaac ttcactttgt gctaaatggt attgtttcaa agcgttctcc
     2281 cgtaaagact ggtttagatc ttccgccatt ttattcatgc tagcggtaag tgtttgtaat
     2341 tccagtgttt ccggcagggt taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac
     2401 atctcgatca gctgattgat cggttcttgg acctttctct ctaaaaagcg acgattcttt
     2461 ttcgcataga aaaaaatggt actaagaact ccaagttcca ctattgttaa tagaatgagc
     2521 gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga tctccacacg aacaaaatct
     2581 tgtaagatat ctgacagcag ttgagtgaca gaatctacct gattgatgat ctcttcattg
     2641 gtatccataa aatcttttgc caagatatta ttgatcagct cgttttgata atcttccagt
     2701 gtatcgatcg tacgtagcga tacatctaac gtactaattt ctttaagtgt atttgtatta
     2761 tttttgatct ctcgaatatc attcctaagt tcttctagga tattttcctc attgaactca
     2821 tctacggtta cttggccaga aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc
     2881 ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg tctgttgata aatcaaaagc
     2941 tgttgcgaat ataacagtat gctcagtagc aaagggataa cagcaaatag aagcatggct
     3001 ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt ttgtcatgat acttctccag
     3061 tcaataattt ctggttggga tgttagctat attctgctgg gaaaagacag tctcggaaat
     3121 aaaaatatct ttaggtaatg tttttcctgt ttgaatattt ccaaaataac gagtaacggt
     3181 attggctaca taccagccga catcaggttt acactcgact acacaattga cgattcctga
     3241 gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt acaatgatca aatcttctcc
     3301 aggtttgatt gatgtttctt ttatcgcagc taaagcaccg tgagtcattt catcactatg
     3361 gctgtataat acatcgattt cggataaatc atttgtttct aaatatcgtt tcatctggtc
     3421 ttttcctttc atccgaataa aatcaccgac aattcggtga acaatagtca tcggaggttt
     3481 atggtcgatg gttccatctt tttttagggt tggtttacga ttgattgatt ccaaaaatcc
     3541 ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca gcaagttcta ggattctgat
     3601 ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat aaacccgccc gatttccttc
     3661 tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg
     3721 tctgtcgaca agtatgacag ggatccctgc tcgctttgct tcttttaata cagagtccca
     3781 cccggattct tgcaaaggag taaaaatgat catatccact ttataggcaa tgaacgtccg
     3841 tatatcttgg atttgccgtt cctgattcat gtagccattt cggtagagaa cttggtaatc
     3901 ttctttttca agcgcagtga taattgattc agtatgcttt ttccgccagc tactctccgt
     3961 tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct tcctttgtat taggtgtaca
     4021 ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa aataaaacac gtttcacttt
     4081 aaatcactcc atctggattt gttagtacta tcttatcaga taaaagggaa aatcaaagtc
     4141 aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa atccaagaca aaaaaagtac
     4201 acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg ttaacgcttt ctttattagc
     4261 tatactcggt gtatcaattg aaatggggga taaaaaatga aaaacaaatt tagaaagatt
     4321 gttgggacta tcgctatttt gaccgcaagc attctattaa gtgcgtgtgg caattcagga
     4381 tctgcagatg atagtacggg gtatgtagga atcgctatgc cgaccaaatc agcagaaaga
     4441 tggatcgctg atgggaacaa catggtctcg gaattagaaa aactgggtta taaaacagat
     4501 cttcaatatg gagaagataa agtagaaaat caagtagcac aaatagaaaa tatgattaca
     4561 aaaggggtag atacacttgt gattgcttct atcgatggtt cggctttaac tgatgtgtta
     4621 gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg atcgtttact gatgaattca
     4681 gaaaatgtcg attattacgc gacttttgat aattttggag taggtgtatc gcaagcagct
     4741 tatattgaag agcacttagg attgaaagaa ggaaaaggac cattcacgat tgaattattt
     4801 ggtggctcgc ctgacgacaa caacgcacta atcaattaca acggagtgat gtctgtttta
     4861 caaccatata tggacaacgg tcagttggtc gtgccatctg gacagacgag tttcagtcaa
     4921 atcgctacgc ttcgctggga tggttctacg gcgcaagcaa ggatggataa tctgttaagc
     4981 gcaaattata ctgaccaaac attggatgct gttttgtcgc catatgatcc aataagttta
     5041 ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg
     5101 attactggac aagatgcgac agttgctggc gttaaatcga tcattgccgg ggaacaaaca
     5161 caaacgatct ttaaagacac acgtatctta gcaaaaaata cgattgaaat gatcaaagca
     5221 atcagtgatg gagaagaggt tccggtcaat gatactgaaa cttatgataa tggggtgaaa
     5281 actgtcccta cgtatttagc aaatacagta tctgtcgata aagataatta tcaagcagag
     5341 ctgatcgaca ccgattatta taaagaatcc gatctgaaaa attaagagga accaaacaag
     5401 gaactaaaga gatctaagtc agagagagga taagaattat ggcggattgt attttaaaaa
     5461 tggcagatat caccaaaaaa ttttctggaa tccgagcgtt agattgcgtg aatctgatga
     5521 ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga
     5581 tgaatgtctt gagcggcttg tatccatatg gctcctatga cggggaaatc ttttatgatg
     5641 gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga aaaaggcatc gtcatcattc
     5701 atcaagaatt agcactaagt ccttatcttt caataaaaga gaatattttt ctcggaaatg
     5761 aacaagcaga aagaggcgtg attgattggg aactgacaga aaagaaaaca tatgaactgc
     5821 tgaataaagt cggtttgtct atcgacccga atcttctagt atcccagatt ggcgtagggc
     5881 aacagcaact agttgaaata gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg
     5941 atgagcctac cgctgcattg aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag
     6001 agttgaaaga gcaaggtgta acctccatca ttatttcaca taagctgaat gaaatcgtgg
     6061 atatcgcaga tcgaatcacc attttacggg atggacagac gattgagaca ctagaaaatg
     6121 aaatgatcag cgaagagcat attattcgcg ggatggtggg cagagattta gttgatcgtt
     6181 atcctgaaag aaaaccggat attggagaag tttattttaa agtagaagac tggacggtct
     6241 atcaccctct tgatcacgag cgaatcataa acgaccacat caacttgcat ttgaaaaaag
     6301 gagagattgt aggaatagct ggactgatgg gagcgggcag aactgaattc gctatgagtc
     6361 tatttggaca ttcttatgga agcaagatat ccggacgtat attaaaggaa ggggcagaga
     6421 tatcagttaa gaccgttcct tcagccattg caaacggact cgcttatgtc tcggaggacc
     6481 gaaaatcttt aggactgaat ctgctgatgg atattcggga aaacgtgacg ctgagcagtt
     6541 tggataaaat aagtcaaaaa ggagtattag ataaagaaaa agaagtaact gaggcagaaa
     6601 atttccggaa aaagatgcgt ataaaaacaa actccatcca tcaaattgtc agtagtctta
     6661 gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct gatgacacaa cccgatattc
     6721 tttttttgga tgagccaacc cgtgggatag atgttggggc aaaatatgag atctacacga
     6781 ttatcgaaga aatggcagca aatggcaaag ctgtctgtct tatctcttct gaacttcctg
     6841 aaatattagg gatgtgtgat cgaatctata cgatgaacga aggacgtatt acaggagaag
     6901 tctcgcgaga agatgcagat caggaagtct tgatgaaatt aatgacgaaa gaagaggtgg
     6961 gttaagttgg aacaggcaaa aaatcttacg gataaaaaag agaaaaaact aaatttgaaa
     7021 agtagagcag ctgatctttt tggtcgctac agcatggtga tcattttagc gggactactt
     7081 attgcgtttc aattgatgac ggatggtatt ttctggcgtc cattgaatat aactaacctt
     7141 gttctgcaaa atagccatat attagtacta gctgcaggga tgttattagt tgtgctctta
     7201 ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg ttggtgcaat tgcgggcatg
     7261 atgatggtca atcgccaaat gaacccgtgg atgacagtta tcgcatgtct tgcagtaggc
     7321 gggctgatcg gcgcatggca aggtttttgg gtagcttatg cagggatacc ggcgtttatc
     7381 gtgactttag caggattgct gatgtttcgt ggactgacac aagttgttct aggaggccaa
     7441 tctctagcac cttttccagc tgttttccaa aaaatgtcta cgggttattt accagactta
     7501 agcgaaggaa gcagccttca cttcttgaca ttgattttag gaatgatcat cacgatcgtg
     7561 ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa atcatttatt tgaagtggaa
     7621 tctccaacag ctttcttggt aaaggcggta tttacagcag tcgttgtact aggaataact
     7681 tatatctttg cctcttatca agggtatccg attattttga tcattcttgg tttgatcgtt
     7741 gcagcatatg ggtttttgac aaataaaaca gtagcaggtc gacagattta tgcgacgggc
     7801 ggaaatcgaa aagcggctga actatcaggg atcaaaacaa agcgaatcac gttctgggta
     7861 ttcgtcaaca tgggagtcat ggcagcttta gctggattag ttttagctgc tcgattgaat
     7921 gcggcaacac ctcaagcagg tacatcgatg gaactggatg caatggcggc agtatatttt
     7981 ggtggtgctt cgacttccgg cggcattgga acaatcatgg gtgcgattgt cggtggcttg
     8041 gtcatgggtg tattgaacaa tggcatgtcg attttaggcg tcggggtaga ctggcagcag
     8101 gcaatcaaag gattgatctt gcttttagcc gttgtgctag acttgtataa taagaaaaaa
     8161 cgaacaacgt aggagaacgc cggttattca gaaaaaagac tgtgacaaga catttgtcac
     8221 agtctttttt tcgctaaaag cgaacattca ttgtttcgtt tgtatagact aaaaataata
     8281 tatgtttttt taccattcgt ataaattctc tttttgctct tgattttttt gcttaattga
     8341 gttaaattac atagggcgct aaaaaaagcc ttgattaaat taataatgtt cgtgttttgc
     8401 tggatgtgaa catcaggagg ataatgtaat gaaagtattt gattacgaag atattcaatt
     8461 aattcctaat aaatgtatcg taaatagccg ttctgaatgt gatacaacgg tcacattagg
     8521 taaacataca ttcaaaatgc cagtagtgcc tgccaacatg cagacgatta ttgatgaaac
     8581 aatcgcagaa ttcttagcgg aaaatggtta tttctatatc atgcatcggt ttgatgaagc
     8641 agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga ttgattacct cgatcagtgt
     8701 tggcgtcaag aaagaggaat attcctttat agagaagttg gcagaagaat cgctaaatcc
     8761 tgattatatc actatcgaca tcgcgcatgg acatgctaac tcagttattg atatgattca
     8821 gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc ggtaatgttg gtacaccgga
     8881 agccgtccgt gaattggaaa atgcgggcgc agatgcaaca aaagtcggga ttggtccagg
     8941 aaaagtatgt attacaaaaa tcaagacagg ctttgggact ggaggctggc aactggcagc
     9001 tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc gctgacggtg ggatcagaac
     9061 acatggcgat attgcaaaat ctgtccgttt tggtgcgaca atggtcatga ttggttcatt
     9121 gtttgccggt cacgaggaat ctcctggcga aacaaaagtc gaaaatggaa tcgtgtataa
     9181 agaatacttt ggttctgctt cagaattcca aaaaggcgaa aaacgaaatg ttgaaggaaa
     9241 gaaaatctgg atccaacaca aaggatcttt gaaagataca ctagtggaaa tgcaacaaga
     9301 ccttcaatca tctatttctt atgctggagg aagagatctg gaagccattc gaaaagttga
     9361 ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca atctaaaaaa taaataaaat
     9421 attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata agaacagcca aacaaaaata
     9481 gttctttata tgttttgttt ggctggaatt attatcgcag atacaagctt ttgaacatca
     9541 ttattcagaa taagggactg tgataagatt tttgtcacag tcccttatgt tttgcaaaaa
     9601 aagtttttct tttttctcgt aaattcggta aaatcaaaag aaaaaataga caatagaaaa
     9661 gagaagctta tgtgggaaaa agtagagaaa aaacaaggac cgatttatcg tcaaattatg
     9721 gaacagatta tgagagaaat cgaaaacgga aggatcaatc ctggaagtca acttgaatca
     9781 gaacgaaaac tagggcaact ttttggtgtg aatcgttcca ccgttgtcca tgcattggaa
     9841 gagcttaggg agcttggggt actaacgagc aaacgtggca gcggatggtt tgttaatcag
     9901 actgaatggg ggaaattcgc tgttccaaga atcgattggc ggcaaatgat ctctcctcgt
     9961 tatgagcaga cggactggta tgagcaaaaa ataagagaag caaagcagat ccaaaaaaca
    10021 tcgtttcttg atttatacgc tagcgagatg ccagatgagc ttttgccgaa ttttgagttt
    10081 ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag aaatggatct tttaggctat
    10141 actccgttac gtaacaaaat caaacggtat ttggaaagta aatttgaatt ggcattttcc
    10201 cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa tctttttgat tttacaaacg
    10261 attctttcag ctggagaggg gatagctgta gagtcacctt ctttttttta ccgattacct
    10321 cttttcaaag cttcaggaac gcgtttattt gggataccga tggatgaaga agggatcgac
    10381 ttagaaaaac tgaaagaatc gatccataaa aataaattga aagcagtgct tatcaacccg
    10441 aattttcaaa accctacagg aaaagtgatg agtcaaaaac gacgagaaaa gctagtagaa
    10501 ttgtgtcgac agtatcggct tccaattatt gaagacgatg tatttacgga tctttcattt
    10561 ctaaaccaag aagaagtcct tcctatacga acgattgatc cggataatgt gttatacgtc
    10621 ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg gttgggtgat aggacctgag
    10681 acttttatct cgcaactagc tcaagcgcaa gaaatgatgg agttttcgat gaatatcctt
    10741 acacaaatca cagtggctaa tgtatttaat gaagcaattg atttgaaaat gcaagatgta
    10801 agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt ggggagattc acaatcgttt
    10861 ttcaagcttc attcaatttt aggtggttac tatgcctgga ttacttggga tgggaaaaaa
    10921 ctagaccccg aactagcaga aaaagtagta gatatgggtc ttggaattgc accaagtcta
    10981 ctttttgggc aagatataaa tggaatcaga atcaattaca gtaggctgga tgaaaaccaa
    11041 ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt ggttaacaga ataatttcac
    11101 aaagtaaata gattaaagaa tataacagct tggatttaaa tttcaagctg tttctttttt
    11161 ttcggaaaaa gaatacgata taaagaaact aagaaaaaac cattatttgt acgtccgttc
    11221 acgatgtgta aataaaataa atgtttgcaa caaaactgta taacaccaac gtttttaaat
    11281 agaaacagat ggttgtaagt ttgattagaa aatagaaaaa atgcagatga aatataaaac
    11341 gctttcctta ttgtgatttt attcactaat gctgttaata tgcggattgg cataatttcc
    11401 tttcatcgtg aaaatttgtg cttttagctt gtatttctca taaatccatg ataatttatt
    11461 aatgaagacg gttaaatagt gatacagttt tattcactgt ttcaatcgca gaatgaagag
    11521 attttcttat agatgagaga ggaatgtgtg acaaatggca aacaaaaaag caccgatcga
    11581 cttccaagca ctgctagatg aagtcaatgc agatttccca gtatatcaag cacttgatca
    11641 agatggaaac gtagttaaca aagatttagt acctgattta tccgatgatg aactcgttga
    11701 attaatgact catatggtat ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg
    11761 ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc agctggctag
    11821 tgcttacgca tttgataaag aagatgttct tcttccaggt tatcgggatg tacctcaact
    11881 agtaaaacat ggtttacctt tatctcaagc ttttttatgg tctcgaggac atgctgcagg
    11941 taattcttat ccggaagaat tgaaagctct accaccacaa attatcatcg gtgcacaata
    12001 tgtacaagca gccggtgttg cattaggttt gaagaaacga aacaagaaaa atgtcgtctt
    12061 cacttatact ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc
    12121 tggcgcttac catgcaaatg ccgtatttta tatccaaaac aacgggtatg cgatctctac
    12181 acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg cagctgggat
    12241 tcctggtatt caagtcgatg gaatggatcc gctcgcagta tatactgtat ctaaaatggc
    12301 acgggaatgg gcgatcagtg gaaatggacc agtcctaatc gaaacactga cttatcgtta
    12361 tggaccacat acactatctg gtgacgatcc aacccgctac cgtacaaaag acacagatga
    12421 cgattggcag aaaaaagatc cattgatccg cttccgtaaa tatttgacgg aaaaaggact
    12481 ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc
    12541 tattgctgaa gccgacaaag taccaaaaca aaaggtttca gatttcttga aaaacatgtt
    12601 cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa
    12661 ataatcatgg cacaaaaaac aatgatccaa gcaatcacag atgccttagc acttgaacta
    12721 gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg aggagttttc
    12781 cgggcaactg aaggattgca ggaaaagttc ggagaagacc gagtattcga tactccttta
    12841 gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt
    12901 cctgaaatcc aattctttgg ttttattttc gaagcaatgg acgaagtagt agctcaaatg
    12961 gctcgtacaa gatatcgaat gagcggcact agaaatctac ccatcacaat tcgttcgcca
    13021 tttggtggcg gtgttcatac accagaattg cattcggata atttagaagg tttgatcgct
    13081 cagtctcctg gtattcgagt agtgattcct tccaaccctt atgatgcaaa aggactattg
    13141 atttcttcta ttagaagtaa cgatccggtt gttttcctag aacacatgaa actatatcgc
    13201 tcattccgag aagaagtgcc ggatgaagct tacgaagttc ctttggataa agcagcagtt
    13261 actcgtgagg gaacagatgt atctattatc acatacggag caatggtgcg tgaagcaatc
    13321 aaagcagcag acaatcttgc aaaagaaaat atatctgttg aaatcattga tcttcgtaca
    13381 gttgcgccat tagatgtaga aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt
    13441 gttgtccaag aagcacaacg acaagcgggc gttgccgcgc aagtagtttc tgagatctca
    13501 gaacgcgcta tcctttcatt agaagcccca atcggacgag tttctgcacc tgatacagtc
    13561 ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa
    13621 gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa
    13681 tttaaatt
//
LOCUS       DACEDM010000080        13521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEDM010000080 DACEDM010000000
VERSION     DACEDM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.080"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..384
                     /locus_tag="IWO50_002366"
     CDS             <1..384
                     /locus_tag="IWO50_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0359462.1"
                     /translation="GQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYHK
                     AAACTSEACDFRLWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLKE
                     DFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            630..1226
                     /locus_tag="IWO50_002367"
     CDS             630..1226
                     /locus_tag="IWO50_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0359463.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            1239..2138
                     /locus_tag="IWO50_002368"
     CDS             1239..2138
                     /locus_tag="IWO50_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0359464.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            2141..2626
                     /gene="ssb"
                     /locus_tag="IWO50_002369"
     CDS             2141..2626
                     /gene="ssb"
                     /locus_tag="IWO50_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0359465.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2992..3252
                     /locus_tag="IWO50_002370"
     CDS             2992..3252
                     /locus_tag="IWO50_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359466.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3699..3905
                     /locus_tag="IWO50_002371"
     CDS             3699..3905
                     /locus_tag="IWO50_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0359467.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3905..4129
                     /locus_tag="IWO50_002372"
     CDS             3905..4129
                     /locus_tag="IWO50_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359468.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4144..4581
                     /locus_tag="IWO50_002373"
     CDS             4144..4581
                     /locus_tag="IWO50_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359469.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            4574..5281
                     /locus_tag="IWO50_002374"
     CDS             4574..5281
                     /locus_tag="IWO50_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359470.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            5662..5841
                     /locus_tag="IWO50_002375"
     CDS             5662..5841
                     /locus_tag="IWO50_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359471.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            5934..6119
                     /locus_tag="IWO50_002376"
     CDS             5934..6119
                     /locus_tag="IWO50_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
                     /protein_id="HAQ0359472.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            6153..6584
                     /locus_tag="IWO50_002377"
     CDS             6153..6584
                     /locus_tag="IWO50_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ0359473.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            7521..8795
                     /locus_tag="IWO50_002378"
     CDS             7521..8795
                     /locus_tag="IWO50_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0359474.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            8849..10276
                     /locus_tag="IWO50_002379"
     CDS             8849..10276
                     /locus_tag="IWO50_002379"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0359475.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            10289..11161
                     /locus_tag="IWO50_002380"
     CDS             10289..11161
                     /locus_tag="IWO50_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0359476.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            11256..12251
                     /locus_tag="IWO50_002381"
     CDS             11256..12251
                     /locus_tag="IWO50_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0359477.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(12856..12963)
                     /locus_tag="IWO50_002382"
                     /pseudo
     CDS             complement(12856..12963)
                     /locus_tag="IWO50_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13079..>13521
                     /locus_tag="IWO50_002383"
     CDS             13079..>13521
                     /locus_tag="IWO50_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0359478.1"
                     /translation="MIIKNLGNVKSIDIHVKDLLIFTGKNNTGKTYTSYILYGILSEL
                     FGARFFNYFSHTEIEPHLQNSLNILLVSKDSIRKKFNNDAKKFLEENLKKIAVRNFKV
                     SENNFKNLSFTISDSEISELTGDIFNYEIKRYSISGIVITIQNVG"
BASE COUNT         4798 a         2058 c         2637 g         4028 t
ORIGIN      
        1 ggacaaatcc aatccactga ttttggatcg acgttacaag aggtgaaagc agccagtgaa
       61 aagcaagaag ccgcgcgtca tttagggatt tgtccaaaat gccaggaaca agaagtttta
      121 ctctatcata aggcggccgc atgtacgtct gaagcttgtg attttagatt atggacaacg
      181 attgccaaaa agaaactcac agcgacacag ctgaaggaaa tcattcaaaa tggccgaaca
      241 agccagccag tgaaaggatt aaaaggtcaa aagggttcgt ttgaagcaac gattgttcta
      301 aaagaagact tcacaacgag ctttgaattt tctgaaaaga aaaaaacgaa ctataagaaa
      361 agaacacgcc gcactacaaa ataatggagg ggttatctta tgcgaaatac aacacacaaa
      421 caacaagaaa tcgaaaatca aaaagcaaaa attcgttcag aacaagagaa attacgtgcc
      481 agtaaaagaa gacttcagca attaaagaac cagcaacgga ttcaaaaaga aattgaatta
      541 gatcgataaa atcttcctgg ctattgtata cgcataaact tatgttatac tatatttata
      601 agtttatacg tatacaaagg agggagaaaa tgacaaaagc aatggtttac aagctgctag
      661 aagaatttaa cggaactttg gctttgaagg atgccaaaaa agcgggcatt tctcctgtaa
      721 cgatcaaacg tatggtagat cgtggagagc tggatcgtga gtatccaggt tttttcacct
      781 tacctggtca atttcctgat gaattgttta tggcacaaaa gaaatatgag cgcggcatta
      841 tttcgcatat caccgcgttg gatttatatg atttaacgga tatgatccct agacagatcg
      901 atctaaccgt accttacggt tatcacgttt ctgaaaaggg gttgaaagaa ttcgcagttg
      961 agttacacta ttcaaaagcg gaatggtatg aattaggtaa gatcgaaata aagagtcgtt
     1021 atgggaatcc tgttatagct tatgatccag aaagaacctt gtgtgacatt tggaatccct
     1081 ggtacaacgt tgaggatgaa atcaaagtaa aagcaatcaa aaactacatg gagtcggaca
     1141 gaaaaaatct tagaaaattg aatgagtatc gacgaatttt gcctacagac aaaacgatac
     1201 gctcttatat tatggcttta aattaaagga gaagacacat gacacctacg caattaaaag
     1261 caaaagtgaa gaatatttcc agagaaaaag aagttgatcc gcagcttgtt ttaagacatt
     1321 ttatgatgga aaaattcttg gaaaaaatct cagagtctcc ttatcgtgaa aattttgtgt
     1381 taaaaggcgg cttcttaatt ggctcaaagt atggcattga aaatcgaaca acaaaagaca
     1441 ttgatacgac tttacgggaa atgaaagtaa ccaaagaaac attgacaacg gttctaaacg
     1501 atattttttc aacgccgacg aaagaaggca ttcaatttga gattcaagga atgaaagaaa
     1561 caagggaagc tgattattat ccagggttta gcttacgtgt actggcacat ttggaaaaca
     1621 tgcggccaga ttttaaggta gacgtgacga caggtgactc gatctatcct gcaacgatca
     1681 ctcacagtca taaattgatg tttgaaaatc gaacgattcc tttggaatct tatcctacag
     1741 aacaaattat tgccgaaaaa ttaagtgcaa cctttgattt tttaacagat aattcgagag
     1801 gaaaagattt ttatgatctc tatactattc ctaaaatgga aaagatcgat gagaagacct
     1861 tgaaagatag tgtgagaaat acatttacac gtcgaggaaa aactgatccg ttaaagattt
     1921 attacgaaaa ggatatggat gtactgaaaa atagtcatga gctaaaagat acctggaaga
     1981 aatatcaaaa aagcaatcct tatgctgcgt cgatcacgta cgaagagaca atggattcga
     2041 ttagtaattt gatggaaaaa gtgattcacg tcgaagaaaa agagcgaaaa atagcttatc
     2101 aaagacacca acagatgaac agagaaatgg agagataaaa atgatcaata atgtaacttt
     2161 agtaggacgg ttaaccaaag atccagattt acgttatacc gcaagtggca cagcagtggc
     2221 tacttttacc ctagccgtta atcgtaattt cacgaatcaa aacggcaatc gagaagctga
     2281 ttttatcaat tgcgtgattt ggcgcaaacc agcagaaacg atggcgacat tagctaaaaa
     2341 aggcatcttg atcggcgttg tcggacggat tcaaacacgg acttatgaca accagcaagg
     2401 acaacgtgtg tatgtgacag aagttgtagc tgataacttc caactattag aaagcaaagc
     2461 agcgacagag agccgtgcgc acgctgatca atcgtccacg agtccaagca ccaccacttt
     2521 tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac agaatccatt
     2581 tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttttaagtag cattttctat
     2641 tggttgcatt tgctacggtt tatgttataa tttagtcaac aaaaagaatt ttcaatctat
     2701 aaccaaaaac cactagctgg cgggctagtg gttttctttt acgtttctat tataagaatt
     2761 aacgagtagg cttactgtct ttcttggcaa atttacaatc aagccatttg cagatatagt
     2821 gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc aaacctataa
     2881 cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta tagcataatt
     2941 gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt gatgaacaaa
     3001 tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt ggttcgcttt
     3061 actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga aattatcgat
     3121 aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca cttcaataaa
     3181 cgaaatcagc tggttattgc tagcatgtat gttagaaatc ccgatcattt tacacgatcg
     3241 atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc tctttttgtt
     3301 ttacatagaa acgggaacag gaaaaggcct gtttaggaag gcacaagcgc cttctcaatt
     3361 aaaaaacctt tctctcactc caaccgccac cgccgttcga gaagaaagga agtgaacgct
     3421 gcacgttcac taagatatgg tgtcttgcaa agcaagacaa aataggaaaa gcttcctctc
     3481 accaaaaggt tcgttcctgt tcgctatctc tttattgatt tgccagaccg gcaacaagtt
     3541 gccttttatc accaaaaagc gacaaggaca agggtaaatg gcacgctgcg cgcccttgtc
     3601 cttgtcgctt ttcgttgatt cattctggcc aacaaatcaa taaagaaaga actaacgaaa
     3661 ggaagtaaaa cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa tttgattatt
     3721 ggggtgttcc cttcagcgaa ttattcccaa actatcatgc accacatact gtcgaatgcg
     3781 attgtggaga aagagcgaaa tgtatcaaaa gctatcgttt gtatcaatgt ccaacatgcg
     3841 ggaaaaaata taccttgtct tatggcgatt acattttgat tgatgagaaa ggaaagaaac
     3901 gctgatgaaa acacaacaca aattattgaa tgtaaaagaa gtcatttctg aaaaagtagc
     3961 agaagcttta cttattccgc ggtactggac gatagaaacc cctttagtag aggacgcacc
     4021 gactatgcta tttgaagacc tagtgaagat tgcaggatat gaactgcatt atcaagaaaa
     4081 cttggaaact tatgaaaacg aatattggtt aaccaaatta gagaactaaa aaggagaaaa
     4141 aatatggctc agaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa
     4201 aaaatggagg atatgacgtt cctataccat gaaacacgtg tacccaaaaa acattacaag
     4261 gaaaaattgt cagtcgcagt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt
     4321 gcgacgtact atcgaacatt ggaagaactg aaaaaacaaa atggaaaatg gtttttccaa
     4381 gccttactat gcttggaagc aggcgtcaaa ccaagcacaa tcaaaccttc tgaataccaa
     4441 gctttagaac taacgtatgg aaaatttatc gaaacgaaaa aagccaagac tgtttcaagt
     4501 gaatggttgg actactttga aaatgtcaac aagtatggcg catgttacat catgaaaaag
     4561 gaggacaacg aaaatgagta atgtggttta tcaactaaca gtggaagatg tttctcactt
     4621 tttaacaaac aaagaacatt tgaatgaagt agtcagacgg attcaaaaag actttagttg
     4681 tatggaagaa atgaagtact ttattcaaag agtggaagaa cagttgtata tggaaagcga
     4741 aacgagtcaa acgaaggtca cagtcagcca taatcattta gacgtgtata aacgtcgtcc
     4801 tgaaacggta aaaaaaggaa cagagcttct acgactagaa acagaaaaat acatcatgaa
     4861 tgtgtaccta gaaaggagtg agctggtcga aaactttgtc agctacacga tttgtaggaa
     4921 acactattac tcagaatcag aatattattc aatttttgag agcatggaat taatggaaga
     4981 agaaatggaa gagctaaaca gcaaaaatgc gacggtagac gattattacc ttgtatttac
     5041 tagatttctg acgtgtggga cggaatctat cgtgttaatg ggaatggatt atgaactcgc
     5101 tgtacccgaa gcatttttga gggaatatgt agaagaagta gataacccag gcgacgactg
     5161 gtacacgact ttaactgatt ttttagatac ctacacttac gacgatacag aagaaatcca
     5221 aaatgagctt gactgtaaac gaatgccata tcaacgattt gaaaggaaaa aaggtcaata
     5281 accatttata ccgaaaagtg agaatgtgtg aaataagaac aaaaaacctt gaaattaagg
     5341 gaatggttct tatttcgcac caatcgctcc aaccgccccc gccgctcagg tggtgcgaaa
     5401 taagcgggca acgaccgata acctaagaga aagaaggaaa tccatatgaa taaaatcatt
     5461 cctctgttgt tcgtactgtt tgttggcgag atacttatgc gtcatgctgc agctgtacat
     5521 ttaaacttcg tagtattctt aggaatactg gtattttgtt tgatcttttg gcgatttgta
     5581 ttagcccttg tgagtctact attcaagatg attgcagcca tatttttcat agggttattt
     5641 cgatgaaagg agagagtact aatgccaatt gcaataggaa ataaacgttt acccgtgacg
     5701 ttggatgaaa agagacaaaa agaattgcag gaactcaaac agaagtacgg caaaagtgaa
     5761 tccaggatta tgtgtattgc gttagattta ttgattgcac aagaaaaagc agggtttgat
     5821 gtaccagcac tcaaaaagtg acgtcacttt tcatttgaag aaacaaaagt gatagcactt
     5881 tacgcacgtg tcgatacgtg ttatgattat tttgtaagga ggtatagtgc acaatgccac
     5941 ttacgggaaa agaaatgctt aagttattga aaaaaaatgg ctgggttgag cgaaggcaag
     6001 agggatctca tcatcatctt tataaggatg gtgttagaat cactgttcct gttcatgcca
     6061 atcaagatct tggccgagga cttgagcaaa aaattttaaa ggatgcggga ttgaaataat
     6121 ctcgcattcg attaaagaaa ggattgactg aaatgttaag tgagccttta gcaaaaacct
     6181 atcctgctat ttttagccca gaagaaggtg gtgggtactt tattgaattt cctgatgttc
     6241 aaggtgctta cacaggaatt aacgaagacg atatatctta tggaattgct atggcacaag
     6301 aggtgttagg tatggtttta gcagattata ttgagcatga agatctactt ccagaaccaa
     6361 caccaatcaa taagatttct gttgaagatg attcttttac tactttaatt cgtgtagatg
     6421 ttgccaaata tttgaaagac acagaacttg tcaaaaaaac cttaacaatt cctcaatggg
     6481 cggataagct aggcaaaagg gctggcataa atttttcagt ccttctaaca gaatcaattg
     6541 cagataaagc agataacatt cttcgttctg gaagaaataa ttagaactca aaagagtcag
     6601 aaacttaaaa atctttggct tcttttttta ctacaaagat ggcttcttta aattatttgc
     6661 ctgattgatt taaaaaaatt gcgcaaagac tacgagctaa ttaagttgct cgtggtcttt
     6721 ctttatgaaa actttttaga tctagataaa aacattgaga cactttgaaa aattttttta
     6781 atagtaatgg gcgcttctat gtcaagctga ttcttctttc ggtaaattaa ttttacttag
     6841 aatatcacta tcttgataaa aaatggtgta ttcacaaatt ctggcataaa aaatacctac
     6901 tttctagtta aggttcctga ataagtcgca atccaattat ttccagctga tttataactt
     6961 tttaaatata attttccata ataccaataa ccattacttt tttctgtcca atttcgttat
     7021 tgagaagatc tagaaggcca gccaacttta tcaaaaatca tcattcctgt cactcgtatc
     7081 gttttagaag ctgctcgagt aagtaatgga gaaactgtta aataatttta agtaacagtt
     7141 aagtcgttag ttgggttatc aatggctatt ccgtccttat ctgaacctcg aaacccaatc
     7201 atagaaaaag ttttttgaac aagttttaac agataaatca agttatatac tagaaaattc
     7261 gtaaaatata tttttaatgt cataatcgtt tgatttttct ggattgacat ttatttataa
     7321 aaaatagtgc cattaaaaaa gaaaagtgac acaccaataa aagtcatatc ctgtgacatt
     7381 atatggcatt atccaaatag aataaccggt tgacatgtaa gcggattcct tttataattt
     7441 taggagtata agtccttttc taaaaatatt aaccggttat catgaaaggg gatacaaata
     7501 tatcgttgga ggataaaaat atgtcaaagg ttaacgtatt taaaaatcaa tcctatttac
     7561 aaagttcagc tacattatta ctattttttg cttcttgggg tgtttggtgg tcattttttc
     7621 aactttggct aacatctgaa tcaaatggtt tagggttatc tggcagtgct gtaggaacag
     7681 tattctcggc aaattcgtta gttaccttaa ttttgatgtt tatttatgga acattacaag
     7741 ataaattgta tattaaacga aatttattaa tttttgcttc tgtattagcg acacttgttg
     7801 gaccattttt tatatggata tacgggccat tgctagataa caattttaat ttaggcatta
     7861 ttatgggagc actatttttg tcagctggat atttagcttc tgtaggagtt tttgaagctg
     7921 tgtcagaaag gtttagtcgt ttatttggct ttgaatatgg acaagcaagg gcgtggggat
     7981 catttggtta tgccttggta gcgcttttgg caggattttt atttgtaaaa aatcctcatt
     8041 taaacttttg ggcgggatct ttctttggtt ctttactatt gttaaattta ttattttgga
     8101 accctaaagt tgaacgggaa gcaaatcaaa attttaatca agaacaagct gaatcaaata
     8161 gtattccttc tttaaaagaa atgtttgatc taatgaaact gcctcaatta tggacgataa
     8221 tcatctttat tgtttttaca tggacatttt atacggtatt cgatcaacaa atgtttccgg
     8281 gattttatac tggtttgttt tcaacatcag ctaatggtga aaaaatatat gggacattga
     8341 atgctattca agtattttgt gaagcgttaa tgatgggaat tgttccaatc attatgagaa
     8401 aattaggggt tcgaaatact ttgttattag gtgtaaccat tatgtgtgta cgaattggat
     8461 tgtgcgggtt tgcctcgaca ccattatctg tttcatgcat aaaaatgttg catgctttag
     8521 aagtaccatt atttacatta ccaatgtttc gctattttac acttcatttt gatacaaagc
     8581 tatcagcaac cctctatatg ataggatttc agatagctgc tcaaattggg caagtgattt
     8641 tatcaacacc attgggaata ttaagagaca acgttggcta tcaaccaaca tttaaaatta
     8701 tttctcttat tgtattacta gcaggcatat atgcattctt tattcttaaa caagatgata
     8761 gagatgttca aggggatcca tttattcgag gataatggaa accaacagaa taaaaaacaa
     8821 ttttatctag aaagtgagcg aatatattat gacaaaaaga gctatagaat taacaaatac
     8881 acgttatcgt ttgggatatc atgtgtctgc accatcagga tggattaatg atccaaatgg
     8941 attttgctat tttgatgggt attatcatgt gttttatcaa catcatccat atagtgctga
     9001 atggggacca atgcactggg cacatgctag aagtaaggat ttagttcatt gggaatcttt
     9061 accacttgct ttgactcctg gagatcaaga agatgaaggt ggttgttttt caggaagcgc
     9121 tattgaaaaa aatggtgtgt tgtatctgtt ctatacagga catcactatt atggagacaa
     9181 tgatccagat catttttggc aaaatcaaaa catggcgtat agtacggatg gcattcactt
     9241 tactaaatat gaaaaaaatc ctgtaatagc aaaagcacca gaagataaca cacatcattt
     9301 cagagatcca aaagtatgga aacacgatga aaaatattac atggttttgg gtagtcaaga
     9361 aaaagatgga ttaggtcgtg ctattgttta tcagtcgtta gatttattaa attgggatta
     9421 cattggacca atttctaaag cgaaggaagt acttacagaa ggatttatgt gggaatgccc
     9481 agactttttt gaattaaatg gaaaatctat tcttttatta tcccctcagg gaattgaagc
     9541 aaatggaaaa gattatttaa atctatatga aacaggctac tttatcggtg attatgatta
     9601 tgagacagca aaattcatta gaggaaattt ttatgaatta gataagggac acgatttcta
     9661 tgcaacacaa acaactttat ctccagatgg tagacgtatt gttatggctt ggatggatat
     9721 gtgggaaagc cctatgcctg aaaaagtaga cggatgggca ggagcattaa cgcttcctcg
     9781 agaattagag ttaattggag atcatttgta tatgaaacct gtgagagaat tagaacaact
     9841 tcgagttggc agcggcgttg aaacaaaagt taatttggca ggagaaataa taatcgctca
     9901 agatgcttca ttcaaagaag ctctaattga tgtactattg actaaatcaa aaaatcaaga
     9961 aatcaaattt tcattaaaaa caaaagatga agagttaatt aatatttttt attctaagga
    10021 taaaaatgaa tgtaccttaa ctcgtaacgg caaaaatgat ccaaggtatg gaaccattca
    10081 gatgtgtgat agattatcac ttcgagtgtt tatcgataaa agttcggcag agatttttat
    10141 taatgatggc gagcttgttt ttacagagcg ttattataca gaaaatcaac caagtatgca
    10201 tttgagtgtt agtgaagaag cttttgtctc gagtacaatt tatcatctaa aaaaagatgc
    10261 aatttcttac gagtaaggag gttttatgat gttaattggt agcatagaag caggtgggac
    10321 taaatttgta tgtgccgtgg gagacgagga ttaccgagta caagaccaaa taatttgtcc
    10381 aacgaccacc cccagggaaa cattgaaaaa aactgttgac tattttaaac agtttgatat
    10441 aaatgcatta ggtattgcat catttggtcc aatagaaatt cgaaaaaatt caccaaaatc
    10501 aggatatatt acgtctacac caaaggtggg atggcgaaat gttaatgtgt taggttattt
    10561 acaagatgag ctgaatgttc caatgtcttg gacaacggat gtgaacggat cagcttatgg
    10621 agagtacatc atgtccactt tatcgaatga aaaaataaat tcattagttt attacaccgt
    10681 tggtactggc gttggtggtg gagctgttgt tgacggaaaa ctattaggat cgtttggtca
    10741 ttcagaaatg ggccatacgt ttgtaaaacg ccatcctgat gatattgatt ttaaaggaat
    10801 ttgtccttat catggtgatt gtttagaagg cttagttgcg ggaccaacgt ttgaagcacg
    10861 ttttggaaaa aagggtgaag acgtttcttt aacggatcct atttgggatg taatggccta
    10921 ttatctagca caagctgtga ttcaaacaac attgattata cgaccggata aaatagtatt
    10981 tggtggaagc gtagtaaatg aaaaattgtt agaaaaaatt cgtgttaatg ttaatgaatt
    11041 attggatgat tacgtggaaa taccaccgtt aagtaaatat attacgcgtc ctttaattaa
    11101 aaataatgga tcggcaacac taggaaactt tgctttgggc ttgaatcaat tacaagaata
    11161 aaacatagaa aaaatatcga ttatcaggta tagtagttct atagagttct ttttttcgat
    11221 agttaattat tgcattattt aggagagaca agagaatgcg cccaaaatta gaagatgttg
    11281 ccaaaagagc aaatgtatca aaaacaactg tttcacgtgt attaaacaat cgtggctatt
    11341 taagtcaaaa aacgattgat aatgtttata aggctataga agaactcaat tatcaaccga
    11401 acgttgtagc gcgtcaatta tttcaaaaga aaactaacat tgtcgggtta ctatttccta
    11461 cagtagccaa tccttttttt agtgaactcg ttgaggcatt ggaaaaaaag ttatatgaaa
    11521 ttggttataa ggtattaata ggcaattcga tgaataataa agaaaaggaa acaaattatt
    11581 taaatcagtt attgtctgat caagtagatg gtttaattgt tggtacacat aaccaaggga
    11641 ttcaggaata taaatatcag aatttaccga ttgttgcaat tgatcgagtg atgaatgaag
    11701 atattccagt tgttgaatcc gataactaca atggtggaaa acttgcaacc aagttattaa
    11761 ttgcacaagg tgcaaaaaat attattcata caaacggtcc aattgactta cagactccgg
    11821 ctaatcgtcg gagactagcc tatgaagata ctatgaaagc atatcagtta attccacgaa
    11881 ctgtgacatt agatttcaat atttcttatg taaagaaaaa acagattttt ttccaaatgt
    11941 ttgaggatta tcctaaaata gatggtattt ttgcttcaaa tgacatcgat gcggccctaa
    12001 tattacaagt agcaaaagaa aaggggttaa atgtaccagc tgatttatta gtagtcggat
    12061 atgatggtac tttaatgact cggagtatat taccagatct gacaacagta atacaaccaa
    12121 ttaatgatat agcagacaca gctgttgcta ttttgatgaa acgaataaat aaagaagaaa
    12181 caaaaaagga atatatttta cctgtcacat tatggatggg gaagacatca gtaaagaaaa
    12241 aggacaatta aatttataaa gactatcaat gataatttca ttattaattc gtgatataaa
    12301 aagttgttaa atctcttata atagactatg atacaaaatc atgcttttaa tctgtgtaga
    12361 tgttgccaaa tatttgaaag acatagaact tgtcagaaaa accttaacaa ttcctcaatg
    12421 ggcggataag ctaggcaaaa gggctggcat aaacttttca gtccttctaa cagaatcaat
    12481 tgcagataaa gcagataaca ttcttctttc tggaagaaat aattagaact caaaagggtc
    12541 agaaacttaa aagtttttga tcctttattt tcaagtctga tttgacagat tttcggcagc
    12601 tcatttattt atggtcagaa atagttgctg aaattttaat ctattagcaa atagtccaga
    12661 acgtatgaag aggattcgga tatcatttgt cattaacacc ataaatattt ataaaattcg
    12721 tgttcaccta caattaaatt taatatataa tattcgttgt aagggcttat aattttttgt
    12781 taatgaacaa gatatgtaat tgatagtgtc aatagctatt caaaaatgta ggcttttggc
    12841 ccataaataa caaggttact tatatgataa ctttttttct ttataatctt taatccgata
    12901 gagctttcca gtagtttgaa taatcttagc gtggtgcact aatcgattga ttagtgcatt
    12961 tgtgttcagt ccattgagac ataatagtgg atagttttac tgagaaaatt gataaaaaat
    13021 agagatataa ttcaaatacg tatataatga aactaaagat aaatacggag gaaaatatat
    13081 gattataaaa aacttaggaa atgttaaatc aatagatata cacgttaaag atttgctgat
    13141 ttttactggg aaaaataata cgggaaaaac atataccagc tatattttat atggaatttt
    13201 atcagaactc tttggagcaa gatttttcaa ttatttttct catacagaaa ttgaaccaca
    13261 tttacaaaat agtttaaata ttttattagt tagcaaagac tctataagga aaaaatttaa
    13321 taatgatgca aaaaaatttt tagaagaaaa tttaaaaaaa attgcagttc gtaactttaa
    13381 agtaagtgaa aataacttca aaaatctttc ttttactatt tcagattcag aaattagtga
    13441 gctcactggt gatatcttta attatgagat caaaagatac agtataagtg gcattgttat
    13501 aacaattcaa aatgtaggag a
//
LOCUS       DACEDM010000081        13347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEDM010000081 DACEDM010000000
VERSION     DACEDM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.082"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(46..1365)
                     /locus_tag="IWO50_002384"
     CDS             complement(46..1365)
                     /locus_tag="IWO50_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0359479.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1362..2015)
                     /locus_tag="IWO50_002385"
     CDS             complement(1362..2015)
                     /locus_tag="IWO50_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0359480.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2303..2823
                     /locus_tag="IWO50_002386"
                     /pseudo
     CDS             2303..2823
                     /locus_tag="IWO50_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2725..3441)
                     /locus_tag="IWO50_002387"
     CDS             complement(2725..3441)
                     /locus_tag="IWO50_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0359481.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3491..3700)
                     /locus_tag="IWO50_002388"
     CDS             complement(3491..3700)
                     /locus_tag="IWO50_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359482.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3977..5250
                     /locus_tag="IWO50_002389"
                     /pseudo
     CDS             3977..5250
                     /locus_tag="IWO50_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5357..6388)
                     /locus_tag="IWO50_002390"
     CDS             complement(5357..6388)
                     /locus_tag="IWO50_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359483.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6395..7225)
                     /locus_tag="IWO50_002391"
     CDS             complement(6395..7225)
                     /locus_tag="IWO50_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0359484.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7222..8028)
                     /locus_tag="IWO50_002392"
     CDS             complement(7222..8028)
                     /locus_tag="IWO50_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0359485.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8034..8858)
                     /locus_tag="IWO50_002393"
     CDS             complement(8034..8858)
                     /locus_tag="IWO50_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0359486.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8848..9948)
                     /locus_tag="IWO50_002394"
     CDS             complement(8848..9948)
                     /locus_tag="IWO50_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0359487.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10126..10911
                     /locus_tag="IWO50_002395"
     CDS             10126..10911
                     /locus_tag="IWO50_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0359488.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10944..11327
                     /locus_tag="IWO50_002396"
     CDS             10944..11327
                     /locus_tag="IWO50_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0359489.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11403..11534
                     /locus_tag="IWO50_002397"
     CDS             11403..11534
                     /locus_tag="IWO50_002397"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0359490.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11503..11739
                     /locus_tag="IWO50_002398"
     CDS             11503..11739
                     /locus_tag="IWO50_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0359491.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11817..12788)
                     /locus_tag="IWO50_002399"
     CDS             complement(11817..12788)
                     /locus_tag="IWO50_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0359492.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4057 a         2910 c         2306 g         4074 t
ORIGIN      
        1 aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag aaaatatttt
       61 tattttaaag ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt
      121 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
      181 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
      241 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
      301 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
      361 ctcgtctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
      421 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt
      481 ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc
      541 aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt
      601 ttccagtatt attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa
      661 aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc
      721 aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc
      781 atcattagag ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac
      841 aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc
      901 agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc
      961 atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag
     1021 ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat
     1081 attatctttg atttgtattt cattcttatt atttttttct tttatgaata cttttatttc
     1141 actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat
     1201 attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt
     1261 tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga
     1321 aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta
     1381 tatcctacat ttcttatagt agttatacag tctaattgta atttttttct aagttctttt
     1441 atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca
     1501 ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct
     1561 agttcttttg catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa
     1621 ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa
     1681 gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact
     1741 aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt
     1801 ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc
     1861 tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca
     1921 tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt
     1981 atcatattat catcttcaac agttaatatt ttcatatata cctccactta agcctataca
     2041 ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt
     2101 gccaatactt tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaaaa
     2161 aaggcttcta taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt
     2221 ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc
     2281 accctaccaa aggagtcgct ctatggataa tcatactaga aaattactcg gattaacaga
     2341 taaacattta ttttttgatg aagaatggct gattgaaaaa gaatataaag gcgttcaagc
     2401 gcagtttatt aaagggagat tggatgactc accttcccat tgtgaacact gtggatcgat
     2461 tcgtattatc cgcaacggtt cctatacgac acgtactcag atgttgaaag tcaaagaaaa
     2521 gttgactatt ttagaattaa aacgtacccg ttttctatgc tatgattgtg ggcagacctt
     2581 ttctgcgaaa acggacttag ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc
     2641 gattctcata gaactgtacg aaaatcaatc acgtaatctg aaaatcagaa gaagtaccgt
     2701 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
     2761 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
     2821 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
     2881 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
     2941 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
     3001 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
     3061 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
     3121 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
     3181 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
     3241 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
     3301 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
     3361 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     3421 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     3481 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     3541 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     3601 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     3661 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     3721 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     3781 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     3841 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     3901 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     3961 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     4021 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     4081 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     4141 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     4201 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     4261 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     4321 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     4381 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     4441 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     4501 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     4561 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     4621 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     4681 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     4741 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     4801 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     4861 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     4921 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     4981 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     5041 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     5101 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     5161 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     5221 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     5281 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     5341 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     5401 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     5461 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     5521 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     5581 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     5641 cgtgggtata ttgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     5701 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     5761 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     5821 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     5881 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     5941 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     6001 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     6061 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     6121 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     6181 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     6241 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     6301 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     6361 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     6421 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     6481 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     6541 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     6601 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     6661 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     6721 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     6781 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     6841 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     6901 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     6961 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     7021 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     7081 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     7141 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     7201 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     7261 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     7321 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     7381 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     7441 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     7501 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     7561 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     7621 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     7681 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     7741 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     7801 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     7861 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     7921 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     7981 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     8041 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     8101 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     8161 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     8221 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     8281 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     8341 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     8401 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     8461 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     8521 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     8581 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     8641 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     8701 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     8761 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     8821 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     8881 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     8941 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     9001 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     9061 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     9121 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     9181 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     9241 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     9301 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     9361 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     9421 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     9481 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     9541 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     9601 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     9661 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     9721 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     9781 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     9841 cgattgcttc gttgcttcac ctgagacgca gcctgtcagc aggatactta ctgttgctgc
     9901 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     9961 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
    10021 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
    10081 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
    10141 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
    10201 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
    10261 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
    10321 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
    10381 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
    10441 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
    10501 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
    10561 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
    10621 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
    10681 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
    10741 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
    10801 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
    10861 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
    10921 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
    10981 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
    11041 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
    11101 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
    11161 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
    11221 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
    11281 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
    11341 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
    11401 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
    11461 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
    11521 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
    11581 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
    11641 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
    11701 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
    11761 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
    11821 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
    11881 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
    11941 aaaagctcta ttcttgagct ggctgcagct agtgagtgtc tttcatttat ccataagata
    12001 aagcttggat tcaataaata aataaatgtt ttggttaaag ctgtgaccag caaatgagga
    12061 accgtgcgag ccagttgatc tgccaatgca aacatttctt taggtgaagg cgctggcgtg
    12121 tctgccaacg gatactccag tcccaatgct tggtaaccgt gcataccata gcagtggtac
    12181 ggtagtaatt ctaacggtac gaaccgatca agatctgcca caaaacaagc aatcgtcagc
    12241 agctcggtca ggttcatatt gatacctgga accacaggaa ctcgaattcg cacttcgcag
    12301 ttaggatact caattaaaaa gcgacgaata ttcttcaaaa tttgttggtt atctaagccg
    12361 gtccattgtt tgtggagctt gctggtaaac atttttacat ccacaaacaa tgtatccaca
    12421 tagggagcta ccttttggat ttcattataa gcaccacaaa agctggtttc cagtacggta
    12481 ttgattccaa tgtatttcga tttttgcaaa atttctttag caaaatccgc ctgaaccagt
    12541 gcctcgcctc cgctaatggt cactccgcca tccgagtgaa agtaaaaaac agcatccttc
    12601 tcaatctcat ccattacctc ttcagcggtc atggtctggc caaatccttt ttcaatctgc
    12661 ttgctttgtg attcgggaat cgaacaccag gcacagcgta atgggcagcc ctttaaataa
    12721 acaacggtcc gtaaaccagc accatcatgt agagaaccct gttctattct taatactgtt
    12781 ccttccatat agatttttga attacacact agtttaaact aaaacacaca aggaaaaact
    12841 taccatttaa tcataaaatt agctcatata aatatactag gatgtaaaat agactggtca
    12901 aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat tttaatattc
    12961 taaatcattt tttattggtt tattccctta ttcgatgtag actcaaatca ctatatcact
    13021 agatatcaaa cggcgtcata cggaatcaaa aacatcaaat ttgacctgtt aaatggtcat
    13081 tgaataacat tcaaaatcaa taaaacggag tcagacacgg agtcaaaaac gctgagagta
    13141 aaaaatatat acagccttgt aggtaaagta ccacagggct gtatattttt tatttcagat
    13201 taccttaaat gattccattt actttttatc tcattttttc ctactagtta gtatcaaata
    13261 ctctcctatt ttctcaataa actatttttg gatacatggt atctgaaaca gctaaaacct
    13321 tgtcattaca gcaaaaaatc acgttat
//
LOCUS       DACEDM010000082        13127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEDM010000082 DACEDM010000000
VERSION     DACEDM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.083"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            162..1103
                     /locus_tag="IWO50_002400"
     CDS             162..1103
                     /locus_tag="IWO50_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0359493.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1172..1903
                     /locus_tag="IWO50_002401"
     CDS             1172..1903
                     /locus_tag="IWO50_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0359494.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2047..4227
                     /locus_tag="IWO50_002402"
     CDS             2047..4227
                     /locus_tag="IWO50_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0359495.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4247..4729
                     /locus_tag="IWO50_002403"
     CDS             4247..4729
                     /locus_tag="IWO50_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0359496.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4745..5506
                     /locus_tag="IWO50_002404"
     CDS             4745..5506
                     /locus_tag="IWO50_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0359497.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5637..6971
                     /locus_tag="IWO50_002405"
     CDS             5637..6971
                     /locus_tag="IWO50_002405"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0359498.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7278..7763
                     /locus_tag="IWO50_002406"
     CDS             7278..7763
                     /locus_tag="IWO50_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0359499.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7756..8439
                     /locus_tag="IWO50_002407"
     CDS             7756..8439
                     /locus_tag="IWO50_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0359500.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8565..11210
                     /gene="polA"
                     /locus_tag="IWO50_002408"
     CDS             8565..11210
                     /gene="polA"
                     /locus_tag="IWO50_002408"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0359501.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11255..12091
                     /gene="mutM"
                     /locus_tag="IWO50_002409"
     CDS             11255..12091
                     /gene="mutM"
                     /locus_tag="IWO50_002409"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0359502.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12055..12684
                     /locus_tag="IWO50_002410"
     CDS             12055..12684
                     /locus_tag="IWO50_002410"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0359503.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12736..13068
                     /locus_tag="IWO50_002411"
     CDS             12736..13068
                     /locus_tag="IWO50_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0359504.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         4429 a         2321 c         2896 g         3481 t
ORIGIN      
        1 ttttctgtgc gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac
       61 aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc
      121 tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc
      181 tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct
      241 tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta
      301 ctgtgtctgg aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc
      361 gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt
      421 ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc
      481 aagtgacagg tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga
      541 cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg
      601 tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag
      661 aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt
      721 ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta
      781 cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg
      841 atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt
      901 ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga
      961 ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga
     1021 aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc
     1081 ggtttacagc tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg
     1141 cgctatacta gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga
     1201 tgtccatggc aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca
     1261 tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt
     1321 attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga
     1381 atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg
     1441 tggaaaagaa acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac
     1501 tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg
     1561 ccccttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa
     1621 gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga
     1681 agggaaaaac aatacaggta agacaattgt atttggacat acgattacac caatgctcca
     1741 tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg
     1801 ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga
     1861 tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa
     1921 ggagctgtga ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta
     1981 gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc
     2041 attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca
     2101 gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt
     2161 ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt
     2221 gaaatcgaag agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt
     2281 cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc
     2341 gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa
     2401 gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca
     2461 gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct
     2521 gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca
     2581 aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc
     2641 aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc
     2701 gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt
     2761 ctgaaagtat tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata
     2821 gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat
     2881 aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca
     2941 gatgagcaag caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca
     3001 ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca
     3061 gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca
     3121 gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa
     3181 gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct
     3241 gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct
     3301 tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa
     3361 agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc
     3421 gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct
     3481 gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca
     3541 gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt
     3601 gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca
     3661 cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa
     3721 aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta
     3781 acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca
     3841 cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat
     3901 attttagctg ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta
     3961 ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg
     4021 gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt
     4081 gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta
     4141 ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg
     4201 acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga
     4261 actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca
     4321 agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca
     4381 tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat
     4441 caaacatgaa ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga
     4501 tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac
     4561 gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg
     4621 caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg
     4681 gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag
     4741 gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt
     4801 agccgcacct gaagagggaa attttgagga actgacggca cctacactta ttttagctgg
     4861 aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga
     4921 tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga
     4981 aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct
     5041 aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc
     5101 tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa
     5161 aatcttattg atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt
     5221 atggcaaaat gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca
     5281 cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata
     5341 tttccatgcg ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg
     5401 tccgaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta
     5461 caccagaatt aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc
     5521 gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga
     5581 gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg
     5641 aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg
     5701 ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct
     5761 ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg
     5821 taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc
     5881 agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta
     5941 tccaaaatta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac
     6001 tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag
     6061 gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt
     6121 tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt
     6181 attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa
     6241 aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga
     6301 tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa
     6361 cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac
     6421 cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg
     6481 aaaaactgga tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc
     6541 gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg
     6601 cagagatcaa agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg
     6661 cagttttcca accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag
     6721 cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac
     6781 aaggaaacgt taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca
     6841 aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg
     6901 ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa
     6961 atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa
     7021 aaatcttgcc aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc
     7081 tgcggtaata agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt
     7141 attatttgat gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc
     7201 aagggagcag aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa
     7261 actggagggt ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg
     7321 aaatcgagga aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc
     7381 tgtatttggg attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa
     7441 aaacagaata tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg
     7501 caatttcaaa gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta
     7561 ttgaaccggt tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata
     7621 agatgaagga tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt
     7681 tggatgctgt cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa
     7741 gtgaaggtga attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat
     7801 gcaaaagttt atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg
     7861 gctctttggg tatttccttt gcaattagca acttttatca ataattttcc actaggcttt
     7921 gtgggtctgt ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag
     7981 aatccgagct tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta
     8041 gcggttactc ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact
     8101 ttgatgttca tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt
     8161 ggaagagcag gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc
     8221 attttgagaa gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca
     8281 gggattacag cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca
     8341 gcacatagtg gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg
     8401 ttcttggcgt ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt
     8461 tggggctata ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt
     8521 ctttgttaga atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat
     8581 tattactcgt tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc
     8641 ttgaacgttt caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga
     8701 tgtttgaaaa tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag
     8761 gaaaaacgac ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac
     8821 ctggcgaatt caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac
     8881 agtattacga attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag
     8941 tagacaaaga tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag
     9001 ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata
     9061 caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga
     9121 agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa
     9181 cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg
     9241 aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat
     9301 cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga
     9361 agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc
     9421 agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg
     9481 aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa
     9541 tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga
     9601 aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca
     9661 ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc
     9721 gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg
     9781 atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata
     9841 tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg
     9901 aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat
     9961 tgagccaaga actggcagat aaaaaccaag aagatctttt ccggaaaatg gaactgccat
    10021 tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac
    10081 aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag
    10141 aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa
    10201 aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac
    10261 tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg
    10321 caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag
    10381 tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta
    10441 atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc
    10501 ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac
    10561 ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc
    10621 atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata
    10681 tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac
    10741 tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg
    10801 aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag
    10861 gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt
    10921 acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg
    10981 ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc
    11041 aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac
    11101 ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac
    11161 cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta
    11221 agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac
    11281 cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt
    11341 gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga
    11401 aaccatccaa tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga
    11461 actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat
    11521 cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga
    11581 tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg
    11641 aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa
    11701 tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac
    11761 tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca
    11821 accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt
    11881 tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg
    11941 agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat
    12001 acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc
    12061 gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata
    12121 gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat
    12181 ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta
    12241 tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg
    12301 atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt
    12361 atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt
    12421 gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc
    12481 gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgctg
    12541 gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg
    12601 gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg
    12661 ctttttgcta atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga
    12721 aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca
    12781 aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa
    12841 tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac
    12901 cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca
    12961 cctgatcgta gtaaaacacc gctcccagaa agtgaccaca tttccgatga gcaaaaaatc
    13021 gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa
    13081 tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaag
//
LOCUS       DACEDM010000083        12614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEDM010000083 DACEDM010000000
VERSION     DACEDM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.084"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            19..936
                     /locus_tag="IWO50_002412"
     CDS             19..936
                     /locus_tag="IWO50_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0359505.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3116
                     /locus_tag="IWO50_002413"
     CDS             939..3116
                     /locus_tag="IWO50_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0359506.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3113..4114
                     /locus_tag="IWO50_002414"
     CDS             3113..4114
                     /locus_tag="IWO50_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0359507.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4111..5043
                     /locus_tag="IWO50_002415"
     CDS             4111..5043
                     /locus_tag="IWO50_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0359508.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5288..5404
                     /locus_tag="IWO50_002416"
     CDS             5288..5404
                     /locus_tag="IWO50_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0359509.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5420..7339
                     /gene="tet(M)"
                     /locus_tag="IWO50_002417"
     CDS             5420..7339
                     /gene="tet(M)"
                     /locus_tag="IWO50_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0359510.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7458..7625
                     /locus_tag="IWO50_002418"
     CDS             7458..7625
                     /locus_tag="IWO50_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0359511.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7685..8038)
                     /locus_tag="IWO50_002419"
     CDS             complement(7685..8038)
                     /locus_tag="IWO50_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0359512.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            8543..8965
                     /locus_tag="IWO50_002420"
     CDS             8543..8965
                     /locus_tag="IWO50_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0359513.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            8962..9192
                     /locus_tag="IWO50_002421"
     CDS             8962..9192
                     /locus_tag="IWO50_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0359514.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9653..9856
                     /locus_tag="IWO50_002422"
     CDS             9653..9856
                     /locus_tag="IWO50_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0359515.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            9938..11155
                     /locus_tag="IWO50_002423"
     CDS             9938..11155
                     /locus_tag="IWO50_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0359516.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            11387..11713
                     /locus_tag="IWO50_002424"
     CDS             11387..11713
                     /locus_tag="IWO50_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0359517.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11781..12494
                     /locus_tag="IWO50_002425"
     CDS             11781..12494
                     /locus_tag="IWO50_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0359518.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         4266 a         2193 c         2593 g         3562 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt cactctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtga ttatgaaaaa tcccaaagat
      121 tctgaatcac tggctattga tattctgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtacgaggg
      241 ttgatgaaag tgattgagga attacgggtt gagaatacgc cactaagtac cagtatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcacatt tattattcag taatggttat
      361 gtggagcagt ctatcagctt agaaaaacaa ctgaacatta tacaggttgc ggacttggta
      421 cttcccgaca aggaaacttc ctttgaggaa tataccacta tggagctttt atccgttgct
      481 atgctgattg tcattagtac ctttgcttta gactttatcc atacagaccg aagcattttc
      541 aagattgtag atttagacga agcatggagc tttttacagg tagcacaagg aaaaacacta
      601 tctatgaagc tggttcgggc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagacg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgcattt
      721 cgttccactg accttaacga gattaaaaag accttagcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggcttcc gtggaactgg tcggacggga
     1201 ttggaaaaca ggtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtt
     1261 tgtatgtttc caatgcgaca ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggtaagaata tgcagacctt agctggtgtg agtgcaaacg
     1381 gattttcaac agagggtttc tatgttggat tcctcttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacggga ctgataaaga gagaaaccac aaaggcaatt catgccatta
     1501 tgaattttgt gctggtgttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagtaatgc cagtttatca cttggcacga
     1621 agattgtcat gccccattcc gatagtcaag gcaaggacag cgtggactta atcagagata
     1681 gcctgttttc catacaggtt cagcaaccgt ggctactgct tcaatacaac agttcagaca
     1741 ttgaaagtat cggtattgac cgtgtggaaa gcctgctctc caccagccca gattccaaca
     1801 atggcgaaga cagagaaaaa attgttgcgg aagaaattga agacagaagc aataccaatc
     1861 taaccattac aaagaccatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttca
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtgtgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ttccatgact actctagggg ctgggtctgc cattgttaca ggtaaaaaag
     2401 gacagtcggg ttcggggagt tctgcaagga cacaagcaga tcactcccga ccagacggaa
     2461 aggaaaaatc aacacttgga aaacgtatcg gtcaaaccat cggtacagta gctgatacca
     2521 aagacagaat ggtagacact gctagtggtt tgaaagaaca ggttaaagat ttgccgacca
     2581 atgcaagata tgcagtatat caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa
     2641 ccagtagtat ttctcaaacc aaagcggaca gagccagtgg acgcaaggaa cagcaggaac
     2701 aaaggcgaaa aaccattgcg aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac
     2761 agcctgcttc ttctgttcat gaaagaccga ctacaagaca agaacaatat catgatgaac
     2821 agacctcaaa acagtctaat attcagactt catataagga atctcaacaa gccaaacaag
     2881 agcgtccagc agttaagtcc gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg
     2941 ttcaagaaaa aaccgttcaa aagccagcaa cttcaaccac tacagcagat agaacttcac
     3001 aacgtccaat cacaaaagaa cgtccgtcta ctgttcaaag agtaccacta caaaatacaa
     3061 gaagtagacc accaatcaaa accgccacca ttaagaaagt cggtaagaaa ccatgaagtt
     3121 gaaaacttta gtgattggtg gttctggatt attcttgatg gtcttctcac tgcttctgtt
     3181 tgttgccatt ttattttcag atgaacagga cagcggaatt tccaatattc attatggagg
     3241 tgtgaatgtt tccgcagaag tgctggctca taagcctatg gtagaaaaat atgccaaaga
     3301 atatggcgtt gaagaatatg tcaacatact tcttgcgatt atacaggtgg aatcgggcgg
     3361 tactgcggaa gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag
     3421 tacagaagaa tccattaagc aaggtgtgaa gtatttcagt gaattattag ccagtagcga
     3481 aaggctcagt gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt
     3541 agggtatgtg gctaatcgtg gaaataaata tacctttgaa ctggctcaaa gtttctcaaa
     3601 agagtattca ggtggcgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg
     3661 gggctggcga tacaactatg gcaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt
     3721 cacaacagag tttgatgatg atacggtaca agccatcatg gacgaagcac tgaaatatga
     3781 gggctggcga tacgtttacg gtggagcttc cccgactact tcttttgatt gtagcggact
     3841 gacacaatgg acgtatggaa aagctggaat taacttacca cgaaccgcac aacagcaata
     3901 tgatgtgacc cagcatatcc cactatcgga agcacaagct ggcgatttgg ttttctttca
     3961 ttctacctat aacgctggct cttatattac tcatgttggg atataccttg gcaataaccg
     4021 tatgtttcat gcaggcgacc caatcggtta tgccgactta acaagcccct actggcaaca
     4081 gcatttagtg ggagcaggac gaatcaaaca atgagaaagg aagatttaat gatgaaattt
     4141 agaaaaaatc agaataaaga aaaacagata ccaaaggaaa agaaacctcg tgtctataag
     4201 gtcaatcctc ataaaaaggt tgtgattgcc ttgtgggtac ttttagggct tagtttcagc
     4261 tttgcgatat tcaagcactt tacagctata gatactcata ctattcacga aacaactatc
     4321 atagaaaagg aatacgttga tactcatcat gtagaaaatt ttgtagagaa ctttgcgaaa
     4381 gtctactatt catgggagca atccgataag tccattgata atcgaatgga aagtctaaaa
     4441 ggctatctga cagatgaact tcaagctctc aatgttgata cagtacgcaa agatattcct
     4501 gtatcgtctt ctgtaagagg atttcagata tggacggtag agccaactgg cgacaatgag
     4561 tttaatgtaa cctacagtgt agaccagctc attacagagg gagaaaatac aaagaccgtc
     4621 cactctgctt atatagtgag tgtctatgta gatggttctg gaaatatggt actggttaag
     4681 aatccgacca ttaccaacat acctaagaaa tcaagttata aaccaaaagc cattgaaagt
     4741 gaggggacgg ttgattccat tacaaccaat gaaatcaatg agtttttaac gacgttcttc
     4801 aagctctatc ctacagcgac agccagtgaa ctttcctact atgtgaatga cgggatatta
     4861 aaaccaatcg gaaaagagta catctttcaa gaactggtaa atcctattca caatcgtaag
     4921 gataatcaag tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg
     4981 caggtatctc aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa
     5041 taacaaatat tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa
     5101 attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag
     5161 gagttaataa atatgcggca aggtattatt aaataaactg tcaatttgat agcgggaaca
     5221 aataattgga tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct
     5281 ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat
     5341 gcataaaaat cccagtgata agagtattta tcactgggat ttttatgccc ttttgggctt
     5401 ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc
     5461 aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg
     5521 aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat
     5581 tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga
     5641 cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg
     5701 ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca
     5761 tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg
     5821 aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat
     5881 caaacagaag gtagaactgt atcctaatgt gtgtgtgacg aactttaccg aatctgaaca
     5941 atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc
     6001 attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt gttctctgtt
     6061 ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta tagaagttat
     6121 tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt
     6181 caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt atagtggagt
     6241 actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat
     6301 gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt
     6361 tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc
     6421 acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa
     6481 acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct
     6541 tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt
     6601 acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa
     6661 agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat
     6721 cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt
     6781 gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca
     6841 aaatgcagtt atggaaggga tacgctatgg ttgtgaacaa ggattgtatg gttggaatgt
     6901 gacggactgt aaaatctgtt ttaagtatgg cttatactat agccctgtta gtaccccagc
     6961 agattttcgg atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga
     7021 attgttagag ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc
     7081 atacaacgat gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga
     7141 ggtcattctt agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac
     7201 tttctttaca aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac
     7261 cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat
     7321 gttcaataaa ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat
     7381 aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggag atagttatta
     7441 taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg
     7501 aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa
     7561 ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc
     7621 gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt atggattctt
     7681 ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattatgg
     7741 atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt
     7801 ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt aacgatacaa
     7861 ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta gttaagtacc
     7921 gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt ttcaatcgag
     7981 cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt ttacgcatag
     8041 tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat agaaaaacgt
     8101 gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca ataaaaaaaa
     8161 ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc caaatggcgg
     8221 tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc cttacattgg
     8281 gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca atgtggtttt
     8341 ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc tgtttatatg
     8401 cttttttggc tattcagaac ttttttacaa agtttattta tcagtaatgc aacaaatccc
     8461 cctttcacat tgggactaag agtgaaagga gataaacgag caaggctcac ttcctttcct
     8521 agacagaaag ggggtgagaa acatgaaacc atcttctttt cagaccacaa tagaaaatca
     8581 gtttgactat atctgtaaac gtgctatgga agacgagcga aagaattata tgctttatct
     8641 ttcaaggatt gcaaagcgtg aggtgtcctt ttcggatgtt ggcgattatc ttgttagcca
     8701 gtttgcgaca acagataact attcaactga ctttcagatt tttacactca atgggttatc
     8761 agtaggcgtt gaaaatgatt tgttgagtga agcattacgt gagttgccag acaagaaacg
     8821 tgaaattcta ctgctgtttt actttatgga catgagcgat tcagaaattg cagacctgtt
     8881 gaaattgaac cgttctactg tctatcggca tagaaccagt ggactagcct taattaaaaa
     8941 gtttatggag gaatttgaag aatgaaaaca caatatccta tgattccctt tcctctcatt
     9001 gtaaaggcaa cagatggcga taccgaagcg attaaccaga ttctacatca ttacagaggg
     9061 tacataacga agcgttccct acgacttatg aaagatgaat atggcaatca aagtatggtc
     9121 gttgatgaag tcttacgtgg aagaatggaa accagactga ttacaaagat tttgtcattt
     9181 gaaattaagt aatatcctct ctcctttcgt ggaagcgtgc taaaccattc cacgcttccc
     9241 gaacagggag gtttgttatt ccaccaaagc atattgagct ttcaatgtgt tttgataggc
     9301 taacgagcca ttgttctttg aaaactgaat aaaagtaatc gaatacgttt cgataagaaa
     9361 agagccaacg gaactaaccg ccatgaccta tcttataaag atagcgagcg attcatgtta
     9421 gtgatccgag aagcaatctt tagcaggatt gcctgcaacg acattcttat cgtgataatg
     9481 atactcccat acagtcaata gtccgagcgt gataaaaccg tcgcaggcaa tgagtatggc
     9541 tacatgagaa ccatgcaggg gtggaactcc cgtgagcttt gctaaagctg ttcgattgct
     9601 ggtaaaacaa cttttatgaa atccaaataa gtgatttgga aaggaggatt ttatgaagca
     9661 gactgacatt cctatttggg aacgttatac cctaaccatt gaagaagcgt caaaatattt
     9721 tcgtattggc gaaaacaagc tacgacgctt ggcagaggaa aataaaaatg caaattggct
     9781 gattatgaat ggcaatcgta ttcagattaa acgaaaacaa tttgaaaaaa ttatagatac
     9841 attggacgca atctagcgtc gccaaagggt cttgtatatg ataaaatagt attaagtcgt
     9901 atcaaggctc tttccataaa ggaaaggagc aaatgccatg tcagaaaaaa gacgtgacaa
     9961 tagaggtcga atcttaaaga ctggagagag ccaacgaaaa gacggaagat acttatacaa
    10021 atatatagat tcatttggag aaccgcaatt tgtttactcg tggaaacttg tggctacaga
    10081 ccgagtacca gcaggaaagc gtgattgtat ctcacttaga gagaaaatcg cagagttaca
    10141 gaaagacatt catgatggta ttgatgttgt aggaaagaaa atgacactct gccagcttta
    10201 cgcaaaacag aacgctcaaa gaccaaaggt tagaaaaaac actgaaactg gacgcaaata
    10261 tcttatggat attttgaaga aagacaagtt aggtgtaaga agcattgaca gtattaagcc
    10321 atcagacgct aaagaatggg ctattagaat gagtgaaaat ggttatgctt atcaaaccat
    10381 caataactac aaacgttctt taaaggcttc attctatatt gctatacaag atgattgtgt
    10441 tcggaagaat ccatttgact ttcaactgaa agcagttctt gatgatgata ctgtccctaa
    10501 gaccgtacta acagaagaac aggaagaaaa actgttagcc tttgcaaaag ctgataaaac
    10561 ctacagcaaa aattatgatg aaattctgat actcttaaaa acaggtcttc gtatttcaga
    10621 gtttggtggt ttgacacttc cagatttaga ttttgagaat cgtcttgtca atatagacca
    10681 tcagctattg agagatactg aaattgggta ctacattgaa acaccaaaga ccaaaagtgg
    10741 cgaacgtcaa gttcctatgg ttgaagaagc ctatcaagca tttaagcgag tgttagcgaa
    10801 tcgaaagaat gataagcgtg ttgagattga tggatatagt gatttcctct ttcttaatag
    10861 aaagaactat ccaaaagtgg caagtgatta caacggcatg atgaaaggtc ttgttaagaa
    10921 atacaataag tataacgagg ataaattgcc acacatcact ccacatagtt tgcgacatac
    10981 attctgtacc aactatgcaa atgcaggaat gaatccaaag gcattacagt acattatggg
    11041 acatgctaat atagccatga cgctgaacta ttacgcacat gcaacattcg attctgcaat
    11101 ggcagaaatg aaacgcttga ataaagagaa gcaacaggag cgtcttgttg cttagtagta
    11161 caaatgaatt tactacttat ttaccacttc tgacagctaa gacatgagga aatatgcaaa
    11221 gaaacgtgaa gtatcttcct acagtaaaaa tactcgaaag cacatagaat aaggctttac
    11281 gagcatttaa gaaaatataa aaagataatt agaaatttat actttgtttt atgtaaaaaa
    11341 atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga atagtatgga
    11401 aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg aaaaagattt
    11461 ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat ggaaaacgcc
    11521 ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag atgtattgat
    11581 ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca ttgaagaaaa
    11641 attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac gaaaaaaata
    11701 cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa aacagtaaaa
    11761 tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg ctagtcaata
    11821 tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa cagatcaagg
    11881 attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca aaagaagatc
    11941 tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa ccaatgacga
    12001 cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt gctagtatcg
    12061 cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat acgtcactac
    12121 tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat tatgccttag
    12181 caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat ttagagatgg
    12241 gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca ttttttaaag
    12301 aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa gaaattgtca
    12361 cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa ggagaactgg
    12421 aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc aatctagaga
    12481 caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag ccaaaggata
    12541 tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga ggtgcatctc
    12601 tgatttggtt gttt
//
LOCUS       DACEDM010000084        11848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEDM010000084 DACEDM010000000
VERSION     DACEDM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.085"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..628)
                     /locus_tag="IWO50_002426"
     CDS             complement(5..628)
                     /locus_tag="IWO50_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359519.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(787..2184)
                     /locus_tag="IWO50_002427"
     CDS             complement(787..2184)
                     /locus_tag="IWO50_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0359520.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2216..3187)
                     /locus_tag="IWO50_002428"
     CDS             complement(2216..3187)
                     /locus_tag="IWO50_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0359521.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3561..3986
                     /locus_tag="IWO50_002429"
     CDS             3561..3986
                     /locus_tag="IWO50_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359522.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4474..5175)
                     /locus_tag="IWO50_002430"
     CDS             complement(4474..5175)
                     /locus_tag="IWO50_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0359523.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5191..5631)
                     /locus_tag="IWO50_002431"
     CDS             complement(5191..5631)
                     /locus_tag="IWO50_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0359524.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5631..6206)
                     /locus_tag="IWO50_002432"
     CDS             complement(5631..6206)
                     /locus_tag="IWO50_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0359525.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7054..7533)
                     /locus_tag="IWO50_002433"
                     /pseudo
     CDS             complement(7054..7533)
                     /locus_tag="IWO50_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7692..8393)
                     /locus_tag="IWO50_002434"
     CDS             complement(7692..8393)
                     /locus_tag="IWO50_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0359526.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8501..9910)
                     /locus_tag="IWO50_002435"
     CDS             complement(8501..9910)
                     /locus_tag="IWO50_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0359527.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(9958..11823)
                     /locus_tag="IWO50_002436"
     CDS             complement(9958..11823)
                     /locus_tag="IWO50_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0359528.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
BASE COUNT         3381 a         2552 c         1943 g         3972 t
ORIGIN      
        1 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
       61 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
      121 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
      181 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
      241 tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg
      301 tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga
      361 tattaataaa ttgattttct tcttttcctt ttggcaaata aatgtccaat tcgccttttt
      421 cgtaattggc agttatttta tactccgccc accaataagt tgagaattct tttttgaaat
      481 tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg
      541 tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa
      601 agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat
      661 tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat
      721 tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat
      781 tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat gacccgattt
      841 tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt
      901 atatgcattg gtccacgacc agttatccat acatgtccac atatgatagc ctaaacaatt
      961 ggcaccttct tgaatggctt gatggacata tttcagatga gattggacaa attcgatccg
     1021 ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat
     1081 accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat
     1141 atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg
     1201 catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt
     1261 ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt
     1321 ttctttgata accgtcaaat cctctgcatg gatcacaggc atgagatcaa gttcttttac
     1381 tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt
     1441 gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg
     1501 atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta
     1561 agatttgatg gctaacgctg aagccaacgc ttcatgatag ccaacttgga tcgctttttt
     1621 catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat
     1681 tggttcatta tgagtaaacc atttttttac tttcgaacca aacaattcaa aacaactttt
     1741 ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag
     1801 atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa
     1861 ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc
     1921 aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat
     1981 caactgtaca tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg
     2041 atcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc
     2101 atcgcctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt
     2161 tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt
     2221 tgattccatg tatgatattt tccatcacaa tcactttttc attttcttcc tcttcttttt
     2281 tgattcgttt taacagctgt tctgctgctc gttccccgat tttatctgtt ggtggcgtga
     2341 tcgtgatttt cggaaggata ctcagatcgt ttttatctaa ataatcatag tgactgaacg
     2401 caatatcctt cccgattctg acaccttctt gattgaaaca ttcgatggca ccaatcgaca
     2461 tattgtaatt gctgacaaaa atagcagttg cccctttttc taataatgct tttgccccgt
     2521 aatagccgct ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat
     2581 tttggaccgc ttctttggct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg
     2641 cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta
     2701 cactttctcg atcattcact aatattgtat caatacctgc tggtccttct ggacaattca
     2761 atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg
     2821 ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa
     2881 ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta
     2941 ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa
     3001 gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt
     3061 ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat
     3121 tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccaca tcacggatcg
     3181 atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta
     3241 tgatgaccgc attctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt
     3301 cagtagagca acttcctctc actattaatt atacttaaac aagtatttga accggttcat
     3361 ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt tctgaaagtg
     3421 gcggattttt tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg
     3481 tgcgtttttc tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc
     3541 tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac
     3601 ttttgacatt gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa
     3661 ccgattcttc tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa
     3721 aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg
     3781 aatggacacg cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa
     3841 cagtgctgac agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag
     3901 aaaataaaaa agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg
     3961 aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct
     4021 tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat
     4081 agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa
     4141 ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt
     4201 gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa
     4261 actcactatg gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca
     4321 tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat
     4381 ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt
     4441 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
     4501 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
     4561 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
     4621 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
     4681 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
     4741 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
     4801 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
     4861 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
     4921 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
     4981 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
     5041 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
     5101 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
     5161 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
     5221 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
     5281 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
     5341 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
     5401 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     5461 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     5521 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     5581 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     5641 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     5701 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     5761 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     5821 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     5881 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     5941 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     6001 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     6061 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     6121 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     6181 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     6241 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata
     6301 ggatagacgc cactatatat agtatttaga aaaaaatcat ttttcagcca ttttatagat
     6361 aaaacaaaaa taaataacgt gttttcatcg atatgaaatg acttacttat agaagatact
     6421 gggatttcgt cctatatgcc aataacgtgt attattttaa acaacaaata cttgtttctt
     6481 ttattaacga aaaaatcatt ttgttctcgt gttatgtttc actgataaat gatgtttctc
     6541 taaataaaaa agccgacgaa ctaaacgatc aaaatttgat catttttgtt catcagcttc
     6601 tcctaaaaat ctttttgtta acgataaata gctggatttg taaaaatatc acttgaatcg
     6661 tcactgctgg aagaaaattc agtgataacc gcacctgttg cgcctgcctg gaactagtgt
     6721 ttgatattgg gctcgaacgt gtactgatct ctagcatgaa gaataatttc tttttttact
     6781 gtataataac tttctttcct ttttggaata gtacaggaaa gtttttttgt ttcctttcct
     6841 tcgacataag gtataccaga ccctttcgac aacgaaaggt tctcattttt ccttcatcgt
     6901 catttcaagg agggtgccga tgctctggac agtttcgatc gctcctaaac caaaatctgt
     6961 atattcgatt ctttctaact ctgattcttt cataccattc ctgtctcaag gtacatctat
     7021 tgtacttttt tcttgtaatc cattctgcct ctcctaacta attgtcaaat catcccaaga
     7081 tatgtcttct tcctcttttt cttcttggat gatttctgca ttcggatcta ctggtttttt
     7141 caataataaa atggttattc ctgtgacaaa agaaccagct aacaatgcac caatgaatgc
     7201 ccatggtctg gtcatcgtag cgactgcaaa cattcccgat gccggaatac ggctatctgc
     7261 gccccaaaac atgcttaatc cgccacctac tgtcccaccg actcccgttg ctatgacatt
     7321 gcgtgccaag tcgttcaata cgatagggaa acatccctct gtgatctgac agatccccat
     7381 aggaaaagcg gtcttcaatg tatcgatttc ttgacgattg agtatatttt tacgaatgat
     7441 tttccctaag aaataagcga tcgtcaagcc gaatggggtt accatcgaag ctaaaatcaa
     7501 tacagtgatt ggttcataga ttccttctga aaatcgtgcc acaccccctt atccagaata
     7561 aatggtaatc cagaaacagc cgtgtatctg cggtaataag cctaaccaaa cgaaaaatat
     7621 gagaagctat ttttgtttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     7681 tcttttttta tttatgtttc ataatcaata tggtaaggat gcagctccgt atggtagacc
     7741 gcctccgaat attcaatcat ttccccaatt tgcgtagacg atttccgtat ccgtttgatg
     7801 ccgatctgtg cgtctgtttt cagaattcct tgttcttctt ctgttaaaaa tactgccgag
     7861 aaatcatcag aaaatgagtc aatagtatat ccttttttcg tcagcaaacg atacaaagat
     7921 tctttgccaa attcttctct gctgaattgc atcatttctt gtggaagata atgaacaaaa
     7981 taaatataag gctgattgtt caaataatat aagcgcttaa aacaaacagc ttgctcaccg
     8041 aaaaaggaaa acaaaggatc ttccttcttt atatggacca attgcaaatc cagcgtttct
     8101 ttccggactt catatcggga gttctccaag atttgcgtaa agcttgccgc tttggataat
     8161 cgattgtaag gacgattgct tagaaccgtc gtcccacgac cacttttctt tgcgacatat
     8221 tcatctgatg ccagcagttc gattgccttt cggacagtga ttttgctgac ttgaaacagt
     8281 tcttctaatt cactttctgt cggcaacatc gaaccgactg gatattttcc tgagaaaata
     8341 tcttctttaa ttttatctgc gacttccaag tatagtacac gtgtcctttt cataacgtca
     8401 ccctttgctt tactattact aaaagaatag cacataaaaa ataaaaaaag aacacaaatc
     8461 acatgtgaga agtgtgttct ttttcattca gctgctttaa ttacaagttt acgccatttg
     8521 aggcaattac ttctttatac caatcaaaag attttttctt tgatcgattc agtgtcccat
     8581 ttccttcatc gtctagatcc acatagataa agccgtaacg tttactcatt tctcctgttg
     8641 atgcactgac aagatcgata catccccaag gcgtatagcc catcaaatct acaccgtctg
     8701 ctactgcatt agccatcgct tcgatatgac gacgtaaata atcgatccga taatcatcaa
     8761 taatcgtgcc atcctcttcc atatgatcaa tggctccaag accgttttca acaatgaata
     8821 atggtttctg gtaacgattg tataattcat tcaatgcgat tctcagccct tctggatcga
     8881 tctgccagcc ccattcactt gcctcaagaa acggattttt tactcctccc agcaagttcc
     8941 cttctgaagt tgctgcttgt ggatcagtct cattgattgc cgtagacata taataactaa
     9001 atccgatgta gtccactgga tattctttta gcaactggaa gtctttttcc gtttgctcta
     9061 atttttccgg atcgactcca tattttttat gggtacgcgc tgtgtatgca gggtattctc
     9121 cacgtacttg gacatccgtg cagaaaaaat tgaattcttg gttttggatc attgttgctg
     9181 cctgattgac tggattggca tcgatgctgt aagttgttgc atagataatc atgcagccga
     9241 tttggatatc cgaacgcaat tcgtggccga tttttacggc tttgctactt gcaacgaatt
     9301 gattatgcca tgcttggaaa atattttggt actctcctgc tccattgctt tttactaatc
     9361 cttgacttaa agctggaaag tgaaaggccg aattgatttc attgaaagtc atccaatatt
     9421 tgactttact gctgtatctc tctaatactg tctgagcaaa tcgttcatag aagtcgatca
     9481 gttttcgatt tttccagcca ccgtattctt ttgccaaatg aagaggcatt tcgtaatgag
     9541 aaatcgtgat cacaggttca atatcatatt ttaagcactc atcaatcagc tgatcataaa
     9601 attctaatcc cgcttcgtta ggagtacctt cgtctccatt tggaaaaatt cgtgtccagg
     9661 caatcgaaaa acgataacat ttgaatccca tttccgcaaa caaggcaaga tcttctttga
     9721 aacgatcgta atgatcgatt cctcgatgat tcgggtaaat atatttttct tgatcaatcg
     9781 tccagtcaaa ttcttcactg ccgataatcg caaagcgctg cttacctcca ggcatagcat
     9841 ctgcaacgga tagtcctttt ccgtctcgat cataagcccc ttctaattga tttggcagca
     9901 gttgctccac cccataaaaa tttttctgaa aaacgtgatt gcatcctgat ttcctcctta
     9961 ttttacaatt gccagaacat cttcgtttgc aataatctct ttttggtcta attcaagtat
    10021 atcggcaaat gcatcagtat tcgtaatgac aactggagtg attacgctat agccagctgc
    10081 tctaattgct tcgatgtcaa aacgaatcaa taaatctcct gctttgacga gatccccttg
    10141 tttgactaag atctcaaagc cattcccatt taattctact gtatccatcc cgatatgaat
    10201 caaaatctcc gctccgtcag tcgttgtgat ccctacagca tgtccagttg gaaaaatagt
    10261 cgtgatctct ccgtctgcag gtgcaactag ttctcctact tctggatcaa tagcgatacc
    10321 ttttcccatc acgccagaag aaaaaacttg gtctggtact tcactcaatg gcagtacttt
    10381 acccgttaaa ggacttttta aggtatgccg tgctgaagta atcggttctc ctgcattcgc
    10441 atgtttgttt tctaattgat tgtcttgtgt ttgttcgtca aatcctaaaa tcaatgtacc
    10501 tacgaatgca agaacaaaag caattcctgt agctattact gctactgtca catttgactc
    10561 tacaccatcc accgtattaa tgaaagtcgg aatcgtcaat aagctgacca aactgctgga
    10621 gaaagctttt actccggaaa atccgataat agctccacca atacctccag aaatacaagc
    10681 ggcaatgaat ggtttcttca aaggtaatgt cacaccatac acagttggtt cagtgattcc
    10741 aaataacgaa gtcactgtag aagaaatccc taatgctcga agtttcgtat cttttgttcg
    10801 caatgctaca gctaaagcag ccccaccttg tgccaagatg gctggaagaa gcattggcat
    10861 caatacatca aacccatatt gttcgatatt caaaaacatg atcggtacga atccccaatg
    10921 catgccgaac atcacgaata cttgccataa acttcccatg attagacctg ctacgattgg
    10981 actaaatcca taaatcgagt tgaagagacc gcctaaagca ttaccggcaa ccgtcccgat
    11041 tggtcctaaa gcaagaaatg ttaatggaac catcactact aaaacgacaa gcggaactaa
    11101 aatcatttgc agaaattgcg gaatcacttt ttttacgaat ggctctaatt tactttgaac
    11161 ccatacggct agaatgattg gtatgacaga agaagtataa ccagatggtg aaagaatcac
    11221 tgggatccca aagaaactga tagcttctcc cgcaccagca actgctgtga tagctggata
    11281 cacaagtgcc atagcaattg ctactgctaa aaactggttg gctttgaact ttttagctgc
    11341 tgtaaaagca agcatgacag gtaagaaggt aaagacacca tctgctgcag caaacaatat
    11401 ttgataagta ccgctatcag ctgtcagcca gcccatcacc gttgccaatg ataagaatcc
    11461 tttcaatacc ccggcggctg ccatcgcccc tagaaaaggt gtaaatatac tagagataat
    11521 atcgatcaga cgattcaaaa tatttttgga ctcttctttt ccatcctccg aaactgcaag
    11581 accttcctca tccacgatcg attgataaac atctgctaca ttactgccaa tcactacttg
    11641 atactgaccg ccactttgta cgacttggat cacgtccggg tcttctttta acttttgcgt
    11701 atctgctaaa gattcatctt ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact
    11761 tttgacattc ttttgtcctc ctaccgcttc ccatacacga cgaccaactg cttgattatc
    11821 cattgttttt tccttctttc ttttttat
//
LOCUS       DACEDM010000085        11746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEDM010000085 DACEDM010000000
VERSION     DACEDM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.086"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            472..1035
                     /locus_tag="IWO50_002437"
     CDS             472..1035
                     /locus_tag="IWO50_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359529.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1096..1440)
                     /locus_tag="IWO50_002438"
     CDS             complement(1096..1440)
                     /locus_tag="IWO50_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359530.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1537..1782)
                     /locus_tag="IWO50_002439"
     CDS             complement(1537..1782)
                     /locus_tag="IWO50_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0359531.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1852..2106)
                     /locus_tag="IWO50_002440"
     CDS             complement(1852..2106)
                     /locus_tag="IWO50_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359532.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2270..2593)
                     /locus_tag="IWO50_002441"
     CDS             complement(2270..2593)
                     /locus_tag="IWO50_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359533.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2780..2853
                     /locus_tag="IWO50_002442"
     tRNA            2780..2853
                     /locus_tag="IWO50_002442"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2814..2816,aa:Ile,seq:cat)
     gene            complement(3238..3735)
                     /locus_tag="IWO50_002443"
     CDS             complement(3238..3735)
                     /locus_tag="IWO50_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0359534.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLFPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3775..4584)
                     /locus_tag="IWO50_002444"
     CDS             complement(3775..4584)
                     /locus_tag="IWO50_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0359535.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4918..5775
                     /locus_tag="IWO50_002445"
     CDS             4918..5775
                     /locus_tag="IWO50_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0359536.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5768..6670
                     /locus_tag="IWO50_002446"
     CDS             5768..6670
                     /locus_tag="IWO50_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0359537.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6703..7053
                     /locus_tag="IWO50_002447"
     CDS             6703..7053
                     /locus_tag="IWO50_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0359538.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7087..8025
                     /locus_tag="IWO50_002448"
     CDS             7087..8025
                     /locus_tag="IWO50_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0359539.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8103..8996
                     /locus_tag="IWO50_002449"
     CDS             8103..8996
                     /locus_tag="IWO50_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0359540.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9034..9597
                     /gene="amaP"
                     /locus_tag="IWO50_002450"
     CDS             9034..9597
                     /gene="amaP"
                     /locus_tag="IWO50_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0359541.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9611..9799
                     /locus_tag="IWO50_002451"
     CDS             9611..9799
                     /locus_tag="IWO50_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0359542.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9812..10714
                     /locus_tag="IWO50_002452"
     CDS             9812..10714
                     /locus_tag="IWO50_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0359543.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10746..10988
                     /locus_tag="IWO50_002453"
     CDS             10746..10988
                     /locus_tag="IWO50_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0359544.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11244..11615)
                     /locus_tag="IWO50_002454"
     CDS             complement(11244..11615)
                     /locus_tag="IWO50_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359545.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4168 a         1981 c         2219 g         3378 t
ORIGIN      
        1 aaacaatttt gtggagcgtg aagcttttat ggtagaggaa aatatgaata aagtgatgtt
       61 gataaaaaga ttcctatctt gaatatgaat cgagatgtct tgaaaatgaa tgagtttgac
      121 caatcattat cctatcgaaa tttacagttt cgttcgattg gtctgtcaat taatgaaaaa
      181 gtaaaaaaga atatgttcta ggaagagggc ggcacatcta aatttttaag taacggacag
      241 ttcaaatatc aagcattgaa agaggctatc ctactgtttg aaggaatacg aatatataaa
      301 aacgacaaag aggaaaaagt agttcattaa atcccttcaa gtgatgaaag aatgagatct
      361 aataaaacct gatctttcat ctgttttttt aataacgtaa taattttttt attttttgtg
      421 ctttcgccgt tattgtagag tgatttagca ttcttatttt taaaaataac tgtgttatat
      481 attaaaaaag gcataatagt tataagcttt tttaaagaat atttagaaat tattaatttt
      541 cgtctgaaaa caaaaaaaga aagcgttatt attttagtct attttcaggg ggggaacctt
      601 atgataaaaa aactattttt actatcaata ttaacgctag caatggcagg ttgttctgac
      661 tctggcttag caactgaact gatcacaaaa tcaactgaag ccacgacaga ggagttaaaa
      721 tgtggaaacg atcacagtga ctacagaggc ttaaatcgac gatgcatggg aatattaggc
      781 agaagcaatt gtaagcatcg atgtagtaat cgaagaaggt gtagaagaag caacagaaaa
      841 atcaaaagaa agatcaaaag aagcaatctg tgctgtgcat cgtatacaga cgtcgaagat
      901 gcggtacttt taaaccacgt caggagatgt tcatttatgt ctagggaagt tggcgatgaa
      961 ccaaaactag agtacgcttt tagttttgca cgtgaggatg aagataaccc taaaacttac
     1021 gacattttag cttgatctaa gatggttaaa gctttttaat gaagtaagag gagatagaag
     1081 gcttttctag ttatattagg taagataaat tcctttttgc tccaaatttg ctaaacattc
     1141 tgctagataa acttcatcgt gttccggata aatcggcacg ttgcgctcta cagtaggtaa
     1201 atcaaaaaat gcttcgcatg atttgccacg atacatagga tttttccact ctggtgctgg
     1261 gctgtaacca cgtttttcca tttcatccat gacaagctga tgatattgga ataatctata
     1321 aggagaatag tgaaaaacat aatccacagt agcatgtttc tttccccagc catttcctct
     1381 taatgcacag cattcccgat gttgtcctaa aagctgttga cgaggaagct ttgaaatcaa
     1441 tttttcatgc cataatcaca tagaaatgtc tcctttatat atagattgtt aaccttagac
     1501 gttaaatgaa aaaaccagca ccaaggctgg aaaaaatcac ttcagtttgc ttgctgtttc
     1561 tgagcgacca attaggtaat caacggaaat atcgtaaaag tcagcgatat agattaattc
     1621 aatgatgctt ggaatttctt ctccttcttc gtacttacga tatttgaata aaggaatact
     1681 taacgcgcta gaaacagcta cttgagataa agatcggcca gttcttaaga aattcagacg
     1741 acaacgtaaa gtatccagta aaatagcttc ttcgatactc atttaatcac ctttcattat
     1801 agtagaatag ttataatgac tatagaaagc tacacatgca atcaatagta ctcaatgttt
     1861 tttatttaaa cgtatcaaat aaagaatgac gatcaataaa gacaaaacca gtcctgataa
     1921 ttcttctata aaaacactat cgggttcaaa gtagttgtaa ttaaaaactt tccacataaa
     1981 gtagatagcc ggaagagtca aaatagctaa ataaaaccgt tctttgttcc aattcataag
     2041 ataaacaata cttgcgacca gtgcgaccgc aggcacccaa aacaaaactc tcaccaacgt
     2101 aatcatgtaa cggactcctc aacgattcat ataataaaag ataacatgtt attgcataca
     2161 tcacaaatga aatgctagga aaataaaaga agaatagatc aatgaaaaat gaaaaagcag
     2221 aacagttttc tgctgataag caggaactgt tccgctgaat gtgtcttatc tatcgagaaa
     2281 aaatcaatgg attgatatct aagtaattca atacatataa aaagaggacg atcgcattca
     2341 ctaaaaagga aagaatgatg atcccatagc gaatggaaac atatttaaca aatacttttg
     2401 tttgaatacg ggcatatctt tttcctttat agctgagcat catgacaaga agcaaaaggg
     2461 taatcacacc ttgaaaaatc attccaataa acagaattcc tgaggtttga taatttaatt
     2521 ggagaaaata agctgctgcc gcaactaaat cgaataaaat cgcaataaat ggcaaaataa
     2581 tcactcctaa cattgagaat aaataaaaga ataacatttt ttctaaaata agcaaataaa
     2641 aattaaagta acaaaatagt tatgacacaa gtaaaagaaa taagaataac aatattattt
     2701 tacttatatt ttgaaacttg tgagagtttg tcattcgtgc tatagttgaa aagtaacaaa
     2761 aataaatttt atttatgatg tacctttagc tcagttggtt agagcagacg gctcataacc
     2821 gtccggtcgt aggttcgagt cctacaaggt acataagagg ttgtgaaaaa gctgccctgc
     2881 atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttactt ggctgttctt
     2941 attactgcag atgcaagctt ttgaataccg tttaaggaag aggttgtgaa atcagcgttt
     3001 tgacctgagt attagcttaa caaacggaaa aatagttcct catattttgc cgtttgttaa
     3061 gctaataccg agggtcagct gattgaacac catttaagaa agaggtcgtg agcctgccgt
     3121 ttcactctgt gtcataaaaa agccaatcta ctttgataga aaaagcacgt aggactaaaa
     3181 cagttctgcg tgcttttttt attgtgtgtt aaatatcgta aatgaagata gatattttta
     3241 tgattcgacg atagagacgt tgaaatcttt caacagctct aaagcggatt ctccgtgttt
     3301 tctactggag actaacattg gatcaacaaa aaagtctgct tcaggaaaag cactctgcag
     3361 gcctatcaga ttagagagta cacacaagtt tgtttcaatc ccgactactt cgatctcttt
     3421 ttgttcttct ttgctttcaa ataaagtttc ctggatttca gctaaagttt caggtggaat
     3481 cgtaaagtaa tactttttaa tcactcgttc attttcttgt ggaaaaagaa gagggatcaa
     3541 ttgcaaatct tctcgttctt cttctttgtc cttcagttca actggtatat cccgtgtgaa
     3601 gagaatatat tcattatttt ggcgtgcttt agctaatctt tgagcaatac gttctgccaa
     3661 ttgatctgac tctggaatat aaaattcgct gtttggatca agaatatgat tttgcatatc
     3721 aataataact aacattttct ttcctcctac attcatatcg tcattttatt ctttttaata
     3781 gaagtttttt aaaatataag caacaccagc ttcttcattc gtcagtgtta cttggtttgc
     3841 aatttctttt acttgtcgct gtgcattccc catcgcaata ctcaacccgc tgtaattcaa
     3901 catatcaatg tcgttttctt gatcgccaaa agccatgatg tcactcgatt cgattccaag
     3961 gtaatccgcc agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg tgatttctaa
     4021 gaaatgcgga tcagagaata ctgattggat accatctgct tgcagatctc tttgtagctt
     4081 ctgtagtttt tcttcactct tatctgttaa tgctaattta agatagctaa gagaaggatc
     4141 ttccaacata tttaaaattt gatcaaaaga atggttctgt aaattgaaat gttcataata
     4201 agggtcatct ttgactagat ttgattcatc gaatgtcagc tgatcatctt gggtttctcg
     4261 gctagcaatg atggatattt tcgcttcttg agcatagaag aaggcttttc tcagtgtatc
     4321 gctatctatt tttgaacgtc tgagaacttt cgccattttt ccatccttca tttcagcaat
     4381 cagtgaacca ttggaagaaa tcaaatagcc accgattcct aattcggaaa aaatatcttt
     4441 agcagaaatg ctgttccttc cggtagcgac ggttacgatt ccgccatttt catcaaattt
     4501 ccgaagtgct tggatatttt cttctggtat ctttccattt cgatcaagca aggtaccgtc
     4561 taaatcaatc gctgctagtt tcattttatc ttcctacttt ctttttacat atctattttc
     4621 ctgttacctt aagattaaat gagaatttaa aaaaatgaaa ctaacttgct ttgtcattat
     4681 gaattttttc aaaaaacatt cttaagaaaa aggtggctat atatagtagt ttttggtcaa
     4741 tacctttgaa taaaaagcca tgaaaaacga atagttagcg cttaccgatg cgttttgtga
     4801 taaaaaaatt ttaaaatatt caatgaaaac aaagcatata ttttgcttta aaaccaaatg
     4861 aggaatattc tagatgtaga acaggttaga aaaagattag aaaaaggagt gattttgatg
     4921 gagaagagat acggcgtagt tcctgtggta actgcttcag acgaaaatta tgctccttac
     4981 ttgagcgtga tgatcgcaac tgcattagaa aattgcaata aaacaagacg cataaaattt
     5041 tatgtgattg acgatggtct atcagaatat agtaaagaag gattggaaga aacggtaaat
     5101 aaatattcaa gtaatgcaag tatccaattc ctgacagtgg aaaaagatat ctacgaagac
     5161 ttcctagtca gtgaccatat cacaactacc gcttacttaa gaatttcttt accaaactta
     5221 ttagctaaag aggattataa gaaagtcctt tacttagatt cagatgtgtt agtactagac
     5281 gatatcgtaa aactatatga tgagccgtta aatggaaaaa caatcggcgc tattatcgat
     5341 ccaggccaag tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt gtacttcaat
     5401 tcaggagtga tggtgattga cattgatcaa tggaataaaa aagaaattac agaaaaaact
     5461 atccattacc tcagtgaaaa tggcgatcga atcatctatc acgaccaaga tgctttgaat
     5521 gctgtacttt atgaagattg ggaacaactt catccaaaat ggaacatgca gacttcgctg
     5581 atttttgaac gtcatccagc accaaatgaa aaatatgaac gtcagtataa agaaggaaat
     5641 gaaaaacctt cgatcgttca ctttacagga catgataaac cttggaatac gttgaaagat
     5701 catccgtata ccaatttata cttaaaaaaa ttagcacaca gtactctaac gaaagtaggc
     5761 gagatcaatg aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa aattcgtacc
     5821 acatttagct gcattatttg tctcagtatt agataattgc aacccgtcta aatttgttcg
     5881 gttctatgtg attgacgatg atatcgattt tgaaagtaaa cagcttttac gcttctcagt
     5941 gaaaaacgca cgaatgaact cagatgtgga attcttgaaa atcaataaag aattctttac
     6001 aaatgtggta atcagcgatc gtattccaga aacagcttat tatcgtattg cgattccgga
     6061 attgttccgc ggcacagaag ttgaacgtat cttatatatg gattgcgaca tgatcgcttt
     6121 acaagatatc tctaaattat ggcgtcttga cttcggtgat tcaatcgtag cagctgttga
     6181 agacgctggg ttccatcaac gactggaaaa aatgaaaatt tcagctaaat caatgcgtta
     6241 tttcaactca ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa atattacgca
     6301 aaaagtatta gatttcatcg aacataatcc agaaaaatta cgattccatg accaagatgc
     6361 attaaatgct atcttacatg accgttggtt accacttcat ccgcgttgga atgcacaagg
     6421 ctatatcatg gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg aatacgaaga
     6481 aactcgcaac aatccttaca ttatccattt ttcaggacat gtgaaaccgt ggagcaagga
     6541 ttttgaaggg ccaacgaaga aatattacga aaaatatgca gggatgacgg ctttccgttg
     6601 tgtagctaaa ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa cagatgtagt
     6661 tgttaaataa tagaagacaa caactgaaga gggggagtag gaatggaaat tacaatgtct
     6721 aaaaatgcag tagagacgtt aattgaaaag gttggggaaa atacaaaaat cgcactcgcg
     6781 cttatcaatg attccgatcc atttctaagg gacaaaggtg cttttgcaaa aggcagcttt
     6841 tttcaaatca ttccttttgt ttcggaattt ggagaatatg cgactaaaat cgagcatcca
     6901 ttattggaca tctatacatc taaattagag caaaattatt tcggtaaacg attgaacatg
     6961 gacttcaaca aacagcttga cagcttttca ttagaaaatg aaatcgcagt actcgattat
     7021 aatatcaaat taaagaactg cttcttcagc taatagtttc gagaaagtcc aaaaaggagg
     7081 tatacattga gatactactt tgtggatgaa gaaaagaatg taatagaaac agatgaaaaa
     7141 aaatataatt ggctcgtact tgatagtgca aagcccgaag agattgagca agcaatcgaa
     7201 gaattcgagc ttcctgatga tattttcgta ggaaccgaat atccggaaga agtttctcgg
     7261 atggagcgcc tttatgagac gaaactagag catccatttt ctttggtagt gattaatcta
     7321 gatcagtcag ataatcgtat cgaaaaaaaa ttaacgccaa tatcttttgt actttccgat
     7381 aatctcttga ttacttgtat cgatagaaaa accgaatttg tggatcggct gctgacaaag
     7441 catggaaatc atttaaactc gtttgaaaag atcatcacgt atacattatt agatatttac
     7501 acccattacg tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt ggataaagca
     7561 gctcgtaaaa caacggaaaa tgaagaactg tataaacagg cagatcttga aagagacatc
     7621 gtatatatcg atcatacctt aagagatcaa cgggaaacaa tggatcaatt atggaattct
     7681 gaaactttca tcaaacgttt agacgatgaa cagctactct atgatgtcca tttgagacag
     7741 cgacaaacag aaaaaatgat cgagatctat cgagatttat tagagagtat tggtgatcta
     7801 ttcaacggga tgatggacaa taatctaaat catatcatga aatatctaga ttcagctgca
     7861 ttaatcattt ccgttccggc gttgttcgca gggatttggg gaatgaatac aggaggatta
     7921 ccgggtaaaa gttctgctct aggctttata ctcgtagtct ttggctcttt gatagcagca
     7981 gtagttttag gttaccattt ataccggaaa gattatacaa attaaagaat ctatttaaaa
     8041 caggtatcaa acaaagaaat ttgttttggc aatacaattt aaaaactagg aggaatacag
     8101 caatgacaga accaaaatta atcgacccgc gaaacttgta ccatacggag aaatttccta
     8161 aacaagatca ggatacacca gctttacagc agaatatgat tcctgttcct gattgtggag
     8221 aagaatccta tgaaggcaat aatcaactag aaaaccgccg tgtactaatc actggtggag
     8281 attcagggat cggtcgtgca gcagcgattg catttgctag agaaggggca gatatcgctc
     8341 ttcaattctt cccaggagaa gaaaaagatg cagaggaagt agcaaaatac gtgaaagttg
     8401 caggacgtaa gattgtctta ctaccttttg atcttcgtga cgaacaagca cctaaagaaa
     8461 tcatagataa agccgtgagt gaacttggcg ggctagatac gatggtacta aacgcaggac
     8521 agcaaatttc ttgtcagtct attgctgagt taccacttga gcaagtcaaa gacacattta
     8581 tgataaacat catcgctatg tttgctttag tgaaagaagc tgttccgcat atcccagcag
     8641 gaggagcaat cgtaacaaca acttctgttc aagcattcaa tccaagcgaa catttattag
     8701 attacgcagc aacaaaagct tcaatcgcta actttacgat cggtttagca aaacagcttg
     8761 cacctaaagg aatccgtgta aatggtgtag cacctggtcc aatctggaca ccattacaac
     8821 tagacgaagg acaaccttct gattcgatcc ctgaatttgg tcagcaaagt ttactagaac
     8881 gtgcaggtca gccaggagaa ttagcaccag tttatgtttt cttagcatcg aataaagcaa
     8941 gttatgttac agcccaaatt tacggagtaa ctggtggaga agcgatcaac ttataagaag
     9001 ttgatcgttt cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga
     9061 gtgatcggaa cattactttt gctatctgtg cttttgtttg cgatactatt aagtgcaacc
     9121 tatccattgc cttttcagct agagaagttt cgattctttt ccatcactaa ttcatacgtg
     9181 caacaatatg tattttggct tgccgttgca tttgctattt tgacaattat cacgttactt
     9241 gttttcctgt tttatccaaa aacaatcgga aactttctac tgaaacatgg tgatggggag
     9301 ttgacgttgg ataaaaaagc aattgaagga atggttcgtt ctcacttgca tgaagatgaa
     9361 ttcatccatt caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta
     9421 aaaggggagt tgaaacgaac ttcagcactc attggtaaaa caggaacatt gatgaaagaa
     9481 atcgaaaatg aagtacaaca catattggga acaaaagagc cagtgaaagt atcggtgaaa
     9541 tacacaggct ttgaagaacc agctaaacga agcaatgacc gcaaacgagt agaatagggg
     9601 gggaccaaaa atgaaagaac tattttcgac atacaagctc cctatcatct ttggggtact
     9661 tggattgata ttagccgttt tatggatcac cgtgggcttt ttcaagacct tattgatctt
     9721 gatcatggca gcgatcggcg tcgctatcgg tttttatctg aaacaaacga aaatcttaga
     9781 tcactatttt aaatcttagg aggaaacatc catgaataac aataacaaaa ataataacaa
     9841 taaaatgaac aaccaaaaac cagtgtctgc acatccagac ccaacgacac caaaccaatc
     9901 aaatggtgtg aacgatgaaa agaacaaaac agcaccagta tctgcacatc cagatccagt
     9961 agtccctaac gttcaacaag gagtagaaga gaagaaacat gatgcagaaa agcatgcacc
    10021 tcatcatcca ggaactccag aatctaaagt tccagaacaa catgatgcaa tcgatccttc
    10081 taaacctgtg gaagagcaaa aacatccttc tgaccatgcg aaagcacaag aaaaacttag
    10141 ttctgaccac acaaacggac aaggaaaact tggttctgat catacaaata accaaggaaa
    10201 gcatgattct gaacaggaag attcaatgcg cggtgagctg aattatgaag ataaagttgt
    10261 gcaaaaaatc atcggaatcg caattgaaca agtagacggt ttattatctg caaacggtgg
    10321 tttcttctct aatgtggcag gaaaacttgt gaatacagat aatgtaacgg ctgggatcga
    10381 aacagaagtc ggtaaaaaac aagtagcagt tgatatggat gtcattgtgg aatatggcaa
    10441 agatatcgaa aaaatctttg aagaaatgca agaagtaatc ggcaaagaag taaaaaatat
    10501 gacacattta gaagtgatcg aagtcaacgc taacgtagtg gacatcaaaa caaaagaaga
    10561 atttgaaaaa gatcaagaaa cggtgcaaga caaagtaact gacgcagcga aaaaaaccgg
    10621 tgaatttgct tcgaatcaaa cagataaagt aaaatcagca gctggaagcg gcgcacaaaa
    10681 agtaaaagaa aatacagaac cacgggttca gtaataagga agtaaaacat taggaggaac
    10741 gaaacatggg atttatttgg tcacttatcg tcggaggaat tatcggagca attgcaggag
    10801 caatcacgaa taaaggttct tcgatgggga ttattgcaaa tgtcgtagca ggtttagttg
    10861 gatcagcact tggtcaagca ttattaggtt cttggggacc aagtattgca ggaatggcaa
    10921 tcgtgccatc attgattggt gccatcattt tagtactaat cgtatcattc tttgctcgaa
    10981 aactgtaaaa agacttttaa aaagaggtcg tgaaaaagct gttcttcatc aagtaataag
    11041 aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttgggcatat tatcgaagat
    11101 gcaagctttt gaacactgtt tatacggaat aagaggctgt gacaagactt ttgtcacagc
    11161 ctcttattta tgcgggcaaa ttcccaatta gatttttaaa agtttttcct taaatgtgtc
    11221 tttattgata cagagaagct tctttaataa aacaatgcct ccctaaattt tcttgagata
    11281 ggaaaggtga acatacaaaa taatacgacc actttgaaaa cccagatgat ccacaaatgg
    11341 atcgtatgat actgatgaag taaaaagcaa tggatcagaa actcaaagag attgaaaaag
    11401 acaaggaaga aataatatct cttttttact cttgtctgtt ttagccaata atagctcgta
    11461 aagtaatcta gacaaacaaa tagaagtaaa caaagtttcg taagagcagc aatcgtatca
    11521 tgttgctctg ctagacgatt gacgagcagg aaaaatgttg gtaatggaat atagatcacg
    11581 agtaatgaaa gaaaaaagta aacaataaat ggcatactat tcctcctaat tgtgtgagtt
    11641 cgccctcatt ttaaaataac gatatttgtt tgtgaagctt tttcgtgtcg taacaaatgt
    11701 tatctaatgt atttttaaat aaataatcat tattatcaaa ataatg
//
LOCUS       DACEDM010000086        11508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEDM010000086 DACEDM010000000
VERSION     DACEDM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.087"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..2154)
                     /locus_tag="IWO50_002455"
     CDS             complement(79..2154)
                     /locus_tag="IWO50_002455"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0359546.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IWO50_002456"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IWO50_002456"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0359547.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IWO50_002457"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IWO50_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0359548.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /locus_tag="IWO50_002458"
     CDS             complement(4412..5311)
                     /locus_tag="IWO50_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0359549.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5727)
                     /locus_tag="IWO50_002459"
     CDS             complement(5326..5727)
                     /locus_tag="IWO50_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0359550.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IWO50_002460"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IWO50_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0359551.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="IWO50_002461"
     CDS             complement(6198..8390)
                     /locus_tag="IWO50_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0359552.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="IWO50_002462"
     CDS             complement(8414..9385)
                     /locus_tag="IWO50_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0359553.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="IWO50_002463"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="IWO50_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0359554.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3106 a         2419 c         2103 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacag
//
LOCUS       DACEDM010000087        10993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEDM010000087 DACEDM010000000
VERSION     DACEDM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.088"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..340)
                     /locus_tag="IWO50_002464"
     CDS             complement(<1..340)
                     /locus_tag="IWO50_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0359555.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
     gene            complement(1671..2336)
                     /locus_tag="IWO50_002465"
     CDS             complement(1671..2336)
                     /locus_tag="IWO50_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0359556.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2462..4498
                     /locus_tag="IWO50_002466"
     CDS             2462..4498
                     /locus_tag="IWO50_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0359557.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4502..5140
                     /locus_tag="IWO50_002467"
     CDS             4502..5140
                     /locus_tag="IWO50_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359558.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5168..5596
                     /locus_tag="IWO50_002468"
     CDS             5168..5596
                     /locus_tag="IWO50_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359559.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6053..6964
                     /locus_tag="IWO50_002469"
     CDS             6053..6964
                     /locus_tag="IWO50_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0359560.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            7418..7732
                     /locus_tag="IWO50_002470"
     CDS             7418..7732
                     /locus_tag="IWO50_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0359561.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            7748..8134
                     /locus_tag="IWO50_002471"
     CDS             7748..8134
                     /locus_tag="IWO50_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0359562.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            8163..9548
                     /locus_tag="IWO50_002472"
     CDS             8163..9548
                     /locus_tag="IWO50_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0359563.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            9551..9703
                     /locus_tag="IWO50_002473"
     CDS             9551..9703
                     /locus_tag="IWO50_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0359564.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            9726..10931
                     /locus_tag="IWO50_002474"
     CDS             9726..10931
                     /locus_tag="IWO50_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0359565.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         3727 a         1799 c         2141 g         3326 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaagaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gaggaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagatat catccataaa atggactttg
      421 tctacagtct gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta
      481 ttttacattc taatatattc atatgagcta attttatgat taaatggtaa gtgtttcctt
      541 gtttgtttta gtttaaacta gtgtgtaatt taaaaatcta tatggaaggg tctgtggggt
      601 aatacttaaa taaaggctag gaacctgata acatcaaggt tcctagcctt tttataataa
      661 aatgaagcga aaataatttc ttttaaagag ccgagattat cagctatata agagtgaatt
      721 tgatgcaaaa aacagagccg aaacagtctt ggtgttcgta gctctgctta tatgtttcga
      781 atttaagaca aagagttggc aaaagatgtt gataaggaag ataagataac tgtgttatat
      841 aaactaggta actaactgta acaagctaat tttaagagat tcttattaga agacgtttag
      901 gaatataact aagaaaatga ttatacaggt aattgtcatt tcaataatgt tttctaattt
      961 tcgcgtcttt gttttaagaa aaaggccaga atcagttgga aaaaagttcg tacgatagtg
     1021 aagaggggca aaatttccta ctaatattcc cactaacaag agataaaacg gccaggttgg
     1081 tgtgattcct ctataaaaaa atgcccatgc taccgaaatc ccatagatta acaactgaac
     1141 aatttttaca atgaaaaaat tgttctgcca ttactcaaag tctcttcctc cttgtatcgt
     1201 ttatttgttt cattttatca ttatgatagg cgcttgaaca atccattttg tattttattg
     1261 agtactcttg gataaactac cagcgattat catatttatt tatgttactg taagccatca
     1321 tataaaagta aatgagcaga tcatagaaat ataaaaaaga actaaaattg atctaaatta
     1381 tgtgcagctt gactacgtac caatctatat tacttactag attggtcgtt aatctattcg
     1441 gatcgaatga gcaaaatatt tttgtgaatg tccatccttg attatgattc caaaaagaat
     1501 cattgggagg ctgtggcgta aatattatac tataagccag aaatacctat cattctgaac
     1561 ttttttattt aggaactttc atctcaaggc tttttcaatt aatttgtacc cctgtttttt
     1621 ggataagatc atgtgcattg attttttcta tctctacgtt catctcctac ttaatgccta
     1681 tctggctcct gagggcttct ttttaaggcg tcgtcatagt cttggctgag gatttcttgg
     1741 taatctgagg gaatggcttc ccaagtgagc aggcgaacgt tcatgacgtg ttttcctttg
     1801 tgtttgatta cagcatggtt tttctctttt tctacctctc cagcgtgaaa agacagccat
     1861 gtaccatggc caaagatatc attacatacc caacgttcga tggaattgtc cacgtatcct
     1921 acagggagtg tggtatagcc gatttgtggt ttcaaaaaag gattgaatcg atcgatcgtt
     1981 tcatttttgt attgataatt gataagaaaa agatagacca aacaaaagac gagcagaaag
     2041 aaagatagtg tctttttgag tgagaagggt tgttttgatg aataagattt cttgttaaca
     2101 agaggttcat ctggcaatgt tggttctatt gattcggtag ctgattcaat agaaggaaaa
     2161 tcaatcggca gacctaaagt atgatgtaaa agtgacagca tttcaaggtc tggcattgta
     2221 cgagaatttt cccaactaga aatcgtttta tcggaaacac caattttttc gcctaattct
     2281 ttttgcgtaa gcttacaatt tctgcggtaa gttcgtattt tttctccggt gttcatcttc
     2341 ttctcctttc gattctacag tatatagatt tattctacat gacctataca cttgatagat
     2401 acgttttttt gatgaaaaac ggtatgattt tatcaaagaa tcatttaagt aagggagagt
     2461 ggtgataatc aaaaaaaagt tcacagtttt actcacaatc atagtgaccg gtatcatgag
     2521 tggttttgcc ttggttagta taaataaaca ggtactctat cggacaaatg aatttttcca
     2581 tcaaacagat acttatactc cgattcgatt gccacaacat tttattgggc aagaagaata
     2641 tgagcagcta atcatggtat ttgaaaatgt ctcatcagaa actgagcttt cttatatgaa
     2701 aaaaacagta aatgaatcca atgaagtaga tacatcttgg aacttcaaga tacagccaaa
     2761 gcttgaaatg acattgtatt atcaagggga tgtcaaatct tctcacgaga aggtacccta
     2821 ttctaatacc tcctttcgcg taacgtccat tgaagaatcg atcaaaataa aagattttga
     2881 aggtgaattt ttcttgatga cgagagaaaa agaagcctat gaacggttca tttcactatt
     2941 cactgatcga ttcaatcaag catttcacac tgattatgcc actaatattt ttgaagactt
     3001 tgaaaatagc tattcgtatt cggttatttc aaatacagac gtatataatt atcgagtgta
     3061 tttaactatt ggattgattc ttttattcct tttgttatca tcctttttct tattgatgaa
     3121 taaagaaatc agtttattgt tgaataatga cttttcatta catcaagtat tgtggtgttt
     3181 attggggaaa aagaaaatca ttattacatt cataattgca ggaatcattg gtggttttat
     3241 ttttatgtat tataaggagc aatttcttta tttgttccag atgtacgttt tcttgttcag
     3301 cggaatatat ggtatctctt tgatttctct tttattagtt gaaaaaatcg cttcgttaag
     3361 agaagcgttc aactatatcc aatacattct ttttacattt ggattgttgt tttctcttct
     3421 tttgaccgtt gccaatgtcg aattagcaac tatcgttttt tcttcttcac agttagctgt
     3481 ttcccatgaa ccaccaaaag aaatcgctgg tgaggactat catgtatttt atccagtaac
     3541 catcgggaaa aaccacgtgg attttattta ttccaatcgt tttgcatcag cagcagatca
     3601 ggaattatat gaaactttga ataaaaacgg aagtatatta gtcaatgtat cagattactt
     3661 gaatgaagaa aatgaacagt ttggcagagg gataaaaata aatctgaatt atcttaagaa
     3721 atttcctttg attgatgtct ctggaaggtt gatccaaata accgaaaaag aaacgcaccc
     3781 tgttatattg atccctgaac gatatagaca tacagattta aaaaatgacc tggcgcaaat
     3841 aacagaatat tatcaagaaa tcagtgaaaa agaaccgaaa tttctattta taaaaaacaa
     3901 tcaacccatt tatacattta ttccttctat cccctggatc gaacattatc cggttgttga
     3961 aatactgaca ttgaaaaaca gtatgtattt ggaaagaaat attctcagcg gagaaatcta
     4021 tccaccgtta aagatcaaga taggctcttt gtcgaaagaa aggttatttg agctgattga
     4081 agaaagtcag ttacgtgata acttgcagtt atcatttccg tatactcaga ctgaagagat
     4141 agctgtcaaa gtgttaagca gatcatttca ttacttgata cagatatttt tactcacttt
     4201 cttttctttc ttcttgttga gctatgtcat gttatgcata tattttgtct acaattgtcg
     4261 gaaaatcgct atctttcgtt caagtggtta ttcgctcttt gaaacgtata aagatttctt
     4321 catgatgaac ctcatcaaat ggggaacgac aagtgtaatc ttcctttttc ttatagaaag
     4381 agagcctaaa tatcttttta atattttttt ctttagcttc attgatttta ttctttcagt
     4441 tgtttttatt ttgtctacag aaaagaaaag tcagttattg ctgatgaatg gaggttaaca
     4501 attggagccg ttgatagtga taaaaggtgc gaaaaaaatc attcgaaaca aaacgattct
     4561 tcatttggat cgtttcactg cggatgctgg ggatcttgtg actgttgttg gagaaagtgg
     4621 agcaggtaaa agtacgttat tgaatatatt aggattgaat gatcagttca gttcaggaca
     4681 attctatttg ttcgatcaag agacgaaaca attaactggg agagagaagc gcttgataaa
     4741 aagaaatacc atatcttttc tttttcaaga ttttgggttg attgaatatg aaacaatcga
     4801 ttttaattta gatattggat tgaagttttc gaatttaaat gtttcagata agcgaacagc
     4861 aaagtatgag gcattgaaaa aagtaaatat aaacaagaag ctaagtacgc ctatctacac
     4921 attatctggt ggtgagaagc aaagaatcgc ctgtgcgaga gtcttattga agaatagcaa
     4981 aatcatttta gccgatgaac cgactgggtc attggataaa agaaataagg ataaagtagt
     5041 tgaaatctta tcgcaacaag cttcccaagg tacgttagtt atcgtagtca cgcatgatcc
     5101 atatctgacc aaaataggaa acaagaagat catgctttaa aaatcaaaaa ggaaaggatg
     5161 tcgttttatg aaaaaagcag ttattatttg tgcactaggg ataggttttg ttggaggaaa
     5221 cgctatgtca tctgaagtgg aagcaaattc agttcacggt gaaatctatt acaaatcttt
     5281 aaagccagga gagattcatt ttgatgctaa aggtaatatt attaagtcaa atttaccagt
     5341 agaccgggaa atttctagaa cagcaattaa tcatagctct ggaagatggg tttattatag
     5401 tgatacaact tgtacgagaa aaacaggtca ttctaaccac tatagtcgtg tctataatcg
     5461 tcacggtgca aaagcgaaag ttggaaactc tattcgatca gcaaatgcaa caaaaggagt
     5521 atgggcctat gcaagtgcga caggaaatag aactgacaca ttctcatgtt ggtacaatcc
     5581 aaccggttgg tattaagtat ccaaaagttt atataaaata gaaaaaacat ataatgaaac
     5641 agcgttaaaa gttatttttg gtagttttaa tatattcatt tttagttgta taaaaatata
     5701 tcataataaa aacaacaaaa aaatgtctgg aaaatcgaac ataaaagtgt ctgatttgaa
     5761 tatcccagca gcggaagaag aaaaacaaaa tcgataacat ttgttctaag aattagttac
     5821 gttaaatacg gaacgcaaga tagacttgaa aaacggtaac aaaagaagta gtatattaag
     5881 gtgtgatgtg ctacttgaca tttattctgc tgagcgaact ttctggattg tgtagcctta
     5941 tatctattca ggacgtatcg agcagattag taaaggtgtt cttgatgatt ttcatcttga
     6001 acacctttgt ttttagcaat tcgcaaaaaa cgaaaagtag gagaattgat aaatgaaaaa
     6061 aagattttta gcacttgcca tcgtactagg cactggctta ttgtcaggct gtacgaatgc
     6121 cggtgaaaaa acagccgtta gttataaagg cggtacaatc agcgaacaag aagtaatgga
     6181 tagcttgaaa aaaatgcaag gagcagattc cgctgtgcaa cagttgatcg tttatcaagt
     6241 atttgaagat aaatatggag atgacgtatc aaccaaagaa atcgattctc aatatgatca
     6301 aacaaagaaa caattgggtg attcatttga ttcacaattg aaatctgcag gttacacaga
     6361 acaaacattc aaagacagta tcaaacaaag tctagccttt caagaaggtc taaagaaaca
     6421 tatcaaattg accgatgaag atttaaaaac ggcttgggaa tctttccatc cagaagtgga
     6481 agctcaaatt atccaagtag caagtgaaga tgatgcaaaa gatgtgaaga aagctgctga
     6541 taagggagac gatttctcta aattagccaa agacaagtca actgatacta cgacaaaaga
     6601 agatggcgga aaagtgaaat ttgattccac tactacaacg gttccggcag aagtcaaaga
     6661 agcagccttt aagttgaaag atggtcaagt ttctgatgtg atcacttcta ccaatgcttc
     6721 aacttatact acggaatatt atgtggttaa aatggtgaaa aaccaaaaca aaggcaacga
     6781 catggataaa tacaaaaaag aattgaaaga aattgcaaca gacacaaaat taagtgacag
     6841 taccttccag aataaagtaa ttggcgaagt attgaaagac gctaatgtaa aaatcaaaga
     6901 caaagacttt gaaaatgttt tgagtacatt cacttcagat tctagtacag caagtagtaa
     6961 ataagcggta taaataaaaa atatgaacta tttaaatcaa taaaactgtt catctaaatc
     7021 tttttattag aactaaatca acagtagttt aattactact tgatttagtt ctttttctac
     7081 aaaaaatagc ataaaaatct agttatccgc ataaaaactg gacttatcac actttatcaa
     7141 ggtcaaaacc actcaattta ctactaattt actacttatg aatgagcttt gatacgacga
     7201 tttatccttg aaaagtgaag atataaagat acttccaata aaatttgaat atttaatagg
     7261 tagacacttc aaaaaatgag gtgtctattt ttttacccga ttttgaaagg aagtgaactt
     7321 atgaaaacaa aaaatcaaga atcaaaaggt cgttccccac tctttaagac catcaaacat
     7381 tcattcagcc aataaaaaag aaaggatagg taaaaatatg gaacttaaat ttgtgattcc
     7441 caacatggaa aaaacattcg gcaatttaga atttgctggc gaggataaag tcgttcagcg
     7501 aagaatcaac ggacggctaa ctgtcttatc aagaagctat aatctctatt ctgatgttca
     7561 aagagcagat gatattgtgg tggtgcttcc tgctgaagct ggcgaaaaac atttcggctt
     7621 tgaggaacgt gtgaagttag tcaatccacg tattaccgca gagggctaca aaatcggcac
     7681 tcgtggtttt acaaattacc ttttacatgc tgacgacatg ataaaagaat aaagaaagag
     7741 aggaaaaatg atgagattag caaatggcat tgtattagat aaagacacga cttttggaga
     7801 attgaaattc tctgctctac gtcgtgaagt gagaatccaa aatgaagacg ggtcggtttc
     7861 agatgaaatc aaggaacgta cctatgactt aaaatccaaa ggacaaggac gcatgattca
     7921 agtaagtatt cctgccagcg tgcctttgaa agagtttgat tataacgcac gggtggaact
     7981 tatcaatccc attgcggaca ccgttgctac tgccacctat caaggagcag atgttgactg
     8041 gtatatcaag gcagacgata ttgtgctgac aaaggattct agttcattca aagctcaacc
     8101 acaagcaaag aaagaaccga cacaagacaa atagtcgcta ggtagaaagg agactttttc
     8161 gcatgaaaca gcgtggtaaa aggattcgcc catctggtaa agatttagtc tttcatttta
     8221 cgatagcgtc actcctgcct gttttcctgc tggttgtcgg actgtttcat gtgaagacaa
     8281 tccagcagat caactggcag gattttaacc tatcacaagc agataagatt gacattccct
     8341 atttaattat cagtttcagt gtcgcaattc ttatctgctt gctggtagcg tttgtattca
     8401 aacgggttcg ctatgatacg gttaaacaac tttaccaccg tcaaaaactg gcaaagatga
     8461 tacttgaaaa caagtggtat gaatctgaac aggtcaaaac agagggtttc tttaaagata
     8521 gtgctggtcg tacaaaggaa aagataacct acttccctaa aatgtattat cgacttaaaa
     8581 atggcttgat acagatacgg gtggaaatca cgctgggaaa atatcaagac caactcttac
     8641 acttggaaaa gaaattagag agtggcttgt actgtgagct gacggataaa gagttaaagg
     8701 attcctatgt ggaatatact ttgctctatg acaccatagc cagtcgtatt tctattgatg
     8761 aagtagaagc taaagatggt aaacttcgct taatgaaaaa cgtatggtgg gaatatgata
     8821 agctccctca tatgttgatt gctggtggta caggtggcgg taaaacttac tttatactga
     8881 cactgattga agccttgctt catacagatt caaaactgta tattcttgac ccgaaaaatg
     8941 ctgaccttgc ggacttaggt tctgtgatgg caaatgtcta ctatagaaaa gaagacttgc
     9001 tttcttgcat tgaaacattc tatgaagaaa tgatgaaacg tagtgaggaa atgaagcaga
     9061 tgaagaacta taagactggc aaaaattatg cttacttagg tctcccggca cacttcttaa
     9121 tctttgatga atacgtcgct ttcatggaaa tgctgggaac aaaagaaaac accgcagtta
     9181 tgaataagct gaaacagatt gtcatgttag gtcgtcaagc tggcttcttt ctaatactgg
     9241 cttgtcaacg tccagacgca aaatatttag gcgacggaat ccgtgatcag tttaatttca
     9301 gagtggcttt aggtcgtatg tctgaaatgg gctatggcat gatgtttggc agtgacgtac
     9361 aaaaggattt cttcttaaag cgaatcaaag gtcgtggcta tgttgatgta ggaacaagtg
     9421 tcatatcaga gttttatact ccccttgtac caaaaggata tgatttcttg gaggaaatta
     9481 aaaagttatc caacagcaga cagtccacgc aggcgacgtg cgaagcggaa gtcgcaggtg
     9541 tggactgatc ttgctggctg gtgtggcaat agccacgcca gcacttaacc ccccgtatct
     9601 aacagggggg tacaaatcga caggaaacag tcaaaaaaac attagaaaat cctttggtta
     9661 caagggattt acaaaatttc agcgtatgtc aaatgggctt taaaagttga catacgcctt
     9721 tttgattgga gggattttta ctgaatgaac aaacttggtt acagcattta aaagaaaaac
     9781 gcttggctta tggactatct caaaaccgtt tagctgttgc gactggtatt acaaggcagt
     9841 atctaagcga tattgaaaca ggaaaagtca agccatcaga ggatttacag cagtcccttt
     9901 gggaagctct ggaacgcttc aatcccgacg ctccccttga aatgctgttt gattatgtaa
     9961 ggattcgctt tccgacaaca gacgtacagc aggtggtcga aaacatctta caactgaaac
    10021 tgtcctattt tcttcatgag gactatggtt tctattctta ttcagagcat tatgctttag
    10081 gcgacatatt cgtcctttgc tcccatgaac tggacaaagg agttctggtg gaattgaaag
    10141 gtcgtgggtg cagacaattt gaaagctatc ttctggcaca acaaagaagc tggtatgagt
    10201 tctttatgga cgttttggtg gctggcggtg tgatgaaacg ccttgacctt gccattaacg
    10261 ataagacagg gattttgaat atccctgtac tcactgaaaa gtgccaacag gaagaatgta
    10321 tctccgtctt ccgcagtttt aaaagctatc gcagtggcga actggtacgc aaagaggaaa
    10381 aggaatgtat gggaaacacc ctctatatcg gttcattaca aagtgaagtt tatttctgta
    10441 tctatgaaaa ggactacgag cagtacaaga aaaatgatat tcccattgaa gacgcagaag
    10501 taaaaaaccg ttttgagatt cgattgaaaa atgagcgtgc ctattatgca gtccgtgatt
    10561 tactcgtcta tgacaatcca gagcataccg cctttaaaat tatcaatcgg tatatccgtt
    10621 ttgtagataa agacgattcc aaacctcgtt ctgattggaa actgaatgaa gaatgggctt
    10681 ggtttattgg gaacaatcgt gaacgattaa aactaaccac aaaaccagag ccttactcct
    10741 tccaaaggac gctgaactgg ctatctcatc aagttgcccc gaccttaaag gttgcgatta
    10801 aacttgatga aatcaaccag acgcaggttg taaaagacat tctcgaccat gcgaaactga
    10861 cagaccgaca caagcagatt ttgaagcaac agtcagtaaa agaacaggac gtgataacaa
    10921 caaaaaaata actcaaatac aaattcattg aatatagaga ggagaacatt tttatgaatt
    10981 ttggacaaaa cct
//
LOCUS       DACEDM010000088        10771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEDM010000088 DACEDM010000000
VERSION     DACEDM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.089"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            94..1386
                     /locus_tag="IWO50_002475"
     CDS             94..1386
                     /locus_tag="IWO50_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359566.1"
                     /translation="MFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFLIFS
                     LIMMGAMVLQYNLFQLPLKKTIENTKSVNQSITGELNAHFDQLEIISAYNFENISILK
                     FDSRFKELFNVFKKQNKLLTLGSCLAQVIFQLWIGGIFLIDVILLAKGTISITNFVIV
                     FMYANMVPTLWTTLISIIWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQSL
                     NFSYNKRKVLSNISFSTEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDI
                     NMIDNESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKL
                     GITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYIL
                     NTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            1379..2407
                     /locus_tag="IWO50_002476"
     CDS             1379..2407
                     /locus_tag="IWO50_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0359567.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            2463..2681
                     /locus_tag="IWO50_002477"
     CDS             2463..2681
                     /locus_tag="IWO50_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359568.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            2752..3567
                     /locus_tag="IWO50_002478"
     CDS             2752..3567
                     /locus_tag="IWO50_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359569.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            3631..3966
                     /locus_tag="IWO50_002479"
     CDS             3631..3966
                     /locus_tag="IWO50_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0359570.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCL
                     IVHEVNKQL"
     gene            4390..4653
                     /locus_tag="IWO50_002480"
     CDS             4390..4653
                     /locus_tag="IWO50_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359571.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            4969..5399
                     /locus_tag="IWO50_002481"
                     /pseudo
     CDS             4969..5399
                     /locus_tag="IWO50_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            5538..5960
                     /locus_tag="IWO50_002482"
     CDS             5538..5960
                     /locus_tag="IWO50_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359572.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            6105..6350
                     /locus_tag="IWO50_002483"
                     /pseudo
     CDS             6105..6350
                     /locus_tag="IWO50_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6459..7415
                     /locus_tag="IWO50_002484"
     CDS             6459..7415
                     /locus_tag="IWO50_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359573.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(8251..8922)
                     /locus_tag="IWO50_002485"
     CDS             complement(8251..8922)
                     /locus_tag="IWO50_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0359574.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            9239..>10771
                     /locus_tag="IWO50_002486"
     CDS             9239..>10771
                     /locus_tag="IWO50_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0359575.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEI"
BASE COUNT         3830 a         1447 c         2000 g         3494 t
ORIGIN      
        1 gatttatcaa tgaaaattga ttttcttaag gttttacaaa aaaaaaatct ttttttttcg
       61 caaacgaagc attcaggaga aattcaattt aggatgtttt cggatatttc tactatcgta
      121 aatactatct ttattttttc tgttgaatta cctgtgatag gcgttttagt tcttgtagcg
      181 gtatttatta taagtaaagt gaatcttatt tttctgattt tttcactaat aatgatggga
      241 gctatggtgt tacagtataa tttatttcaa ttaccgctta agaaaacaat cgagaataca
      301 aaaagtgtta atcaaagtat tacaggagaa ttgaatgcac attttgatca attagaaatt
      361 ataagcgcat ataattttga gaatatctct attttaaaat tcgattctag atttaaagaa
      421 ttatttaatg ttttcaaaaa acaaaataaa ttactaacac tgggaagctg tttggcacag
      481 gttattttcc aattatggat tggaggaata tttcttattg atgttatttt attagctaaa
      541 ggaacaataa gtattactaa ttttgtaata gtatttatgt acgctaacat ggtccctact
      601 ttatggacta ctttaattag tattatttgg aaatggcaag actattcagt gtcattaaat
      661 agatattacg aatattattc aaataacaac aaagtggctg gcacaaaaaa aataaataaa
      721 ataaattcta ttactggtca atcattaaat ttttcatata acaaaagaaa ggtattaagc
      781 aatataagtt tttcaactga aatgaataaa ctattcatga tatcaggaga aaatggaaca
      841 gggaaaacaa cactgatgaa attatttact aaacagttgc cggttagtaa taatcaatta
      901 ttcgtcaacg ggatagatat taatatgatt gataacgaat cattaagaag gttaatttca
      961 atcgcaacac aaataccata tataattaat agctccgtga aggagaatat tttaatgggg
     1021 actaaaaact atgataaaaa aaaattcagt aaattaatag aactattggg aataagtaaa
     1081 tttatgacga aattaggtat tactttggac gacaatattt atgatgaaaa aatattatta
     1141 tctggaggag aaaaacaaaa aataagttta gctagggcgc ttattaaatc agcttcagtt
     1201 attatattag atgaaccatt tagaaattta gataatgaaa ctcgcgataa tttgagggat
     1261 tatatactaa atacaaaaaa agaagtttgt tatataatta taactcatga tcacattcta
     1321 gataattttt tggattcaaa tgatgaaata ttatatttaa ataaggagta tgataataat
     1381 gaatagcaaa aaacttcaga tgggtgaaat tgttagaggt cctagtcaaa tatctattga
     1441 tgttacatat aaatgtaatt ttagatgtaa acattgttac aatgcgagtg gtggagacaa
     1501 ttgttttatg gataatgagt tgactgatga tgaactgtta gaacttacaa aagatatatg
     1561 taatttaaag ccatataata tttgtttctg tggaggagaa actttactga gaaaaaaaat
     1621 agttttatct tgtgccactt tattaaaaga ggcaggtatt cctaatattg ctattgttac
     1681 taatggatca ttagtaactc aggatgttgc aaaagaatta ttcaaagcag gtattaatcg
     1741 atttcaagta agtttagatg gaaaaaatgc tagtagccat gaagcactta gattatatcc
     1801 aaacagtttt gaaaaggcaa ttaatgctat caaattattt agagaattgt ccgaagaagt
     1861 ggatgttgct ttttgtccta ctagttttaa ttacaatcaa ttacgagaag tatatcaact
     1921 agtaaatact cttaaagtga agtctttaag aattcaacca tttatgctta gtggtagggg
     1981 aatgcaaaat agggaaatag tgccaactgt ttctcaatat agaaaactag tttctgatat
     2041 taactatatt aaaagaaatg aagattatac caagatagat tggggagatc caattgatca
     2101 tatcatccga cacagaactt tattgaaaga tttttcgaat tttgtttcta ttaggtcaga
     2161 cggaggatta atggcatcac cttacttgcc aattcaagtt ggaaatatta aatgtcacag
     2221 tctttctaaa tattggcgag caggacttta taaagttttt tctttagatt tagttcaaga
     2281 tatagctact caagtttctt caatacaaga tatgagtaaa gaattaaaaa aaggacatat
     2341 taaatggctt aatgaagata tagaaattga tattctcgat gaatacaaac atgatatttt
     2401 aatttaggtt tatcattgga aagtttagat aaacaaagta cttttagaaa ggagggaaaa
     2461 aaatgatttc tgaacctcaa atgaataata tgtatgaaag cccaactata atgggagctg
     2521 gtgaagctgc gccaagagct tgttcagcaa ttatggtgtg tgtgttatta gcatctgcag
     2581 gagtaggtat ccaagcagga gcggtgtata cttatgctgt agttgctaca gtagctgtag
     2641 ctgcagccgc agtttggaaa acagtaggat tagttggtta ggaattaata ggagactaac
     2701 tgtctcctat tgattccaat gattaagcta aggaggaatt ttaagaaaat aatgaaatac
     2761 aataatttaa aaccgaaatt acttaatgag aacatagaat atataagaga agaaaattat
     2821 ggatatttaa ccatagttcc caaaaatgga actgaattga aagataacgt tttcaatcca
     2881 acagtaagat atatattggc taactgtact ggaaaaaaca gtgtaaaaaa aatagcgaat
     2941 ggtttaaaaa agaagtttcc tagtttagaa tatgaaaaat tctatcaaga tgttgtagga
     3001 actattaaat ttttcaaaca ctataatatt attgatatag gagattatag tatggaagaa
     3061 aagaaaatat ataaaattga acaaaattta gaattagaat tactaaatga atcacattat
     3121 gaagaaattt ataattttat cacagatcat cctgaaagta tgtattttca tgaagatatt
     3181 tatcatcaag gaaatagtaa agaattagaa gttgtcctta gatataattt attttcgttt
     3241 acgagtgaat tttacttttt aagacataaa gagagaattg taggaatggt ttctataatt
     3301 gttaatccat ttcaaaactt atcctacagt atagtaaatt acctcatatg tgagcctgca
     3361 tatactacga ctattttgac aacagtatta gaaaatatta acgaagtttc aattttagaa
     3421 gtaaagaagc tagtgattca tttgctaaat tcaacagaag atgaatcact aatcccctat
     3481 ttaaaagatt cacatttttt taaagaagcg gtactatcta aagaatatag agagcatgat
     3541 gttttgagat acgcttacat ttattaaata aaatttctga tagtatttta ataattcgga
     3601 tcatatcata agatatagga gggaaaagaa gtgattttat tgattttgtc tctcgtatta
     3661 ctagttgtat ctaatctaat gaaaaataat cctcctacaa aaattaattc tttttatggc
     3721 tttagaacta aaaagtcaat gaaaaatgaa aaaagttggc atttagcaca aaaattgttg
     3781 ggggattttt caaaaaaaaa agttatccca gtatttttga tgggtattgt ttttttcagt
     3841 atagagattt atagtttagc tattttaaaa aatgaaaatt ttttcgttag tgtattaatg
     3901 ggtgaaagtt taatcctaat actgattttg tgtttaattg ttcatgaagt gaataaacaa
     3961 ttgtgaatta aaaaaataaa aacctagtat aatcacttta ataaattgtt ggtagaaggt
     4021 gatcaaaatg ggagtaaacg aaatgatgaa agtcagattg agtttttaaa tgataccttc
     4081 ctcagaactt caaaacgaat tcaaagattc gttgacaaat cttgagcaag atcatttaaa
     4141 aaaatatttt ctcttcattt gacgaaaaat gtttagaaat cttatataag tgaagataaa
     4201 gtaattggtg taattaattg tagagaatag cctttgagca attgaagcga tacaattatt
     4261 tagaaaatga acttcctcca gcatactaca tgattcctta cgggacgatg ttaaagaaga
     4321 aagcaagaaa ttttagaagt attagtagat aaactaatgg gcttttctca atttaaggga
     4381 gtagcccaaa tgtttatcaa atatggtgca gatacagtag caaccatgtt tacagaagaa
     4441 tatcaagtag actattatga agtaatagat aaatgtgtac attttgttta tgaaaaagtg
     4501 tggaatttta tgaattttgc gataagtgaa gaaatcttac gagtacaaga aaacagaaca
     4561 ggaatattta ccagagacag aaagcttaat ttgattgcgc attatatacg gaatcgctgg
     4621 tcatcaatac caaattttaa ttataaaaaa taattatgaa caatctttta tacaatatac
     4681 acaataaatc aagtagtatt tatgaaaaaa gacttcaaaa acagaatgat tttctatcag
     4741 tcttagaaat tatagagcta aaaattttgg tcacgcgtag aaacgcgttt tttttgtttt
     4801 tttgatagta aagaacgtac aaaacaatga gaatttaaaa gattgtacta ccagtttttt
     4861 taccttttca tttgagttta gagtaagtaa aatattacta taaaaacaca tattcttgaa
     4921 gaaatctttt ttttgctgta ttcatttaga ttatgataga gaaaatacgt gagaggggga
     4981 cctgggatgt ggctatctga tgaaaaaatt cagtggcgta gctatcagaa gcgtatattg
     5041 actaactttg ctacttattt aaataacaat tcagtccatc tagtggctcc accaggggct
     5101 ggcaagacct ttttaggaat tgagatgatc cgacgtttgg attgtaaaac cttaattttg
     5161 gttccttccc taatattgaa gaaacagtgg ttaacaacaa tcgaggaata tttcttagta
     5221 aatccagcag atagttgcta tctttctggg gcgattgaac atcctagtca tattacagtg
     5281 gaaacgtatc agaccattta caaccgactg aaggaggagc caacttatgg tgatcagctg
     5341 gagctgttag ttttttgatg aagctcatca tttaaaaaaa agctggggtg aattgttgat
     5401 tgacttgaag aaaaaacaac cgaatctgtt gacgatttcc ttgacagcca cgcctccgat
     5461 tgatagctct cagacggagt ggaagcaata tattgagtta aaatggcctg attgacgaag
     5521 aaattagcat tcccgagttg gtaaaggagg aggttctatg tcctcatcag gattttctat
     5581 atttgattcc ggctaatcag gaaactactc agcattattt taccttttta gagaaacaaa
     5641 atgagctggt ggaagcgtta atggaaagcc aagagacggt ggattatttg ctttctttag
     5701 aatttgttca gcatccgctt gagcattcga aggaaattta tgaaaatttt gagctgttca
     5761 ctagtgcatt aatttacttg aatcatcata actatgaatt gatacccgaa cattggcagc
     5821 tattaggcag ttctaagcag caaggtaaaa aagcttcagt tccatcttta ggtaaaaaag
     5881 agctgatcac tatttatcag cagatgtttg aagatcaacc ggaattgcct attttttatc
     5941 aattgaaaaa agctggatga ttgtttgatg agtcgctgca tttgtttcca gagtatccag
     6001 acactgttgg tagtcaggcg attcctgaga aaaagcaagc agtttgtcaa attttgttaa
     6061 aagaagaagt tcagctgaag caagaactga cagcggtcat tttgatggat cgtatttgca
     6121 aggaggtatt agcaggcagt gatggtacct tagcttatgg cgtggttcct ttgtttgatg
     6181 aagttgttcg tctattaggt aacactcagc aaatcggtgc catctgtgga gagttcttac
     6241 tgttaccgaa aatgctagta actgttgagc tggcagaact agcgacgggt atgaatgaac
     6301 atccttatat gtcgggttat ttttatttga cggtggatga caaaaaccgc caagcattga
     6361 tgcaagcagt tactcgccta ttaaccgaaa agaagttgaa tattttggta ggaaccgtta
     6421 gtttgctagg agaaggctgg gattgtcctg caattaatct gttgatcttg gctaatcaag
     6481 cttccacttt tgtgcaaaca caacaactac gagggcgggg cttacgccta ggtgcacaaa
     6541 ataaagtaac gaacatttgg catttagcga ttggattacc gggagttcct ctgtctgaac
     6601 agcctgattt gaaaagaatg ctacggcgtt tgggcaatat ttcaggtctg acctttgaat
     6661 cttcaccaaa aattgagagt ggtggcgatc gctttgcttt accactggaa tatacgacca
     6721 ccacgatgga aacttatact cagtcgatgt tctatttttc ttgtgaacga agtcggttga
     6781 aagaacaatg gagtactgct ttagctgatg gctggcacct atctatgcca atcgtcgtta
     6841 ggaaagtcga tcaggtaaag caaaaacaag aaacttcatt gccgatagta gtaaaaaata
     6901 tagagcctct aagcttctgg tcagctatat ttaaaggtaa cttgctggaa tatttccaaa
     6961 ttcgtaggcg acaaaagcta tgggaaaagg aatgtgcccg caaaaaaagc ttggccatat
     7021 gcgttcaaac gacgttgcag cgccttggtg aagttagtga tagaaaagta atcgtagcgg
     7081 ctgatcagca ggaatttaga tgctatcttg agaatgctgg ctatcgagaa ggacagcttt
     7141 ttactgaagc tatgagggag cttttagcac cgctggagaa tccgcggtat ttaatcgaag
     7201 ggaaaaagat agttctgacg gtaccagaac gctttggccg aaataagcaa atcgctaacc
     7261 tttttctaaa cgaattaaat caatcgtcgc tgcccaaatg tcagttggtg tatactagaa
     7321 atcctgaagg acgccaaaag ctagtgactt atcgaatgag gcaagtcttc cagacccgcg
     7381 atgaagtaat tattcaaaaa aacatttgga cgtaagttga tgaaaataaa ttttgctaga
     7441 gtagaacaag tccgagaatt aagttgctac ttaactgata atgccaagga caggagaatc
     7501 ttttcttgac gactgaaaga aactatttga cttatataac ggtgtgtgat gaatatttgc
     7561 aaggcattct cttaaaaagt actcgtaata gtttttttga aaacaaaaca gtactttatt
     7621 aataggactg acagagcgat ataagaacat ctgcagacag aggtgaatgc ttttctcatg
     7681 atcgatgaaa caatggtact tcggatattc ttgcttttag tttgaaccgt tgtgattttc
     7741 tttgattcct aaaagtagtt ttatagaatg aaaatcaagc atacggaatg ctcctttgat
     7801 tgtgttgtgg tgattcaatc taatggagct tctatatgtt tttttatttg caaaaagaac
     7861 cgatagaata tttttatcag tcttagaaat tatagagcca agaaatttca agcacacata
     7921 aaaacgcgtg ttttatgcat tttatgagca aaaataacta aagaccatta cgtaaaacat
     7981 aggtaaatca gcattcaatc tcaaattatg tgatcaaatg ttgatattat ttgattataa
     8041 gttttctttt gctattatca gtctgcaaat aaacagatta tagagtatga atgtagatag
     8101 ttcaatcttt gtttacgacc tcacatgttt ttttactcaa gagaaagtaa tataaagtga
     8161 ctatatttga cagaacttta cataaaaaaa gtagttagtt ttctgtagta atcagaaatg
     8221 taaataaatt aaaaaaaaga aatttagcag ttatatattt ttaaaatatt ttgggtgagt
     8281 agtcaataca acattttgtt catcaagcat taaatgaaga tgcaaggatg ttaagaagtt
     8341 agcttcttca gggtcttgca tggtcaaggc tttaaatagt tgatgatgtt gttccataat
     8401 tttttcccat tttaatcctt ccgtttttac tcgtaaccaa cgaaatcgtt ccaagtgcgt
     8461 attattatct tccaaccatt gccatacttc ttcccgttct gcaatttcaa aacaagcttt
     8521 atgaaataaa ttatcattat gaaaaaaact acgagcattc ttttgactaa cagctttttc
     8581 ttgttctgca attatagttt ctaaaacttg tttcttttga ttattcatct tggcacaaca
     8641 ctctagcata atttgtcgtt ctaaatgttc tctgacaaaa cgagcatttt ttgctaaatc
     8701 taagtcgatt aatgagatat aatttccact ttgaggaatt gtatcaacaa gtccttgatg
     8761 acggagttga atcaatgctt cacgtattgg agtacgaccg atagaaagcg tttcttccaa
     8821 ttttttttct gatacttttt ttccaggttc taagtcagcg tagataattt gctgacgaat
     8881 agattgataa gcttgatttt ggagactagt tttaattgtc atatttaatc cctctcaaaa
     8941 tttcattttt gaatttgata ttttagttct atgctcgtta tataatatca aatttcaaag
     9001 taacatacta gctattaaaa aaacaaaaaa tactcaaata agatcaaaga gaaattaagc
     9061 tttaatttaa gttagttcta aaaaatatat attggtgtgc ttttactaaa aaaagacaca
     9121 ataattctga catggtagaa aaaagaatat ttttagtttt ttcttattga cagcgattac
     9181 attcgatttt atactacaat tgtaacggaa actaacatat cagtatggag gggtaaatat
     9241 ggttttgatt aaagactggc aagattctat cgaaggattg aacaaaatgc aagttcaact
     9301 accgtatttt aatgtcgaag cggtcaaaca agaaggtcag aaacatccag tgtggcttca
     9361 ttttggtgga ggaaatcttt atcgaggatt tcatgcagaa attgctcaat gcttagcaga
     9421 tcaaaatgaa ttagaatccg gagtagttgt gtgtgagaca tttgatgaag aagtagtaga
     9481 aaaagcttat catgactaca acaatgatat tttggagatt gttatgggtg aagatggtga
     9541 actcaaaaaa agacttttag cagtaacagc tgatagttta tattgcaatc caaagaatat
     9601 aacggactat caaagagtgg ttaaatattt tgaagatcct acattacagt ttacaacgtt
     9661 tactattact gaaaaaggat acagtttaaa agattctaaa ggacaatttt tttcggtagt
     9721 ggaagcagat ataaaatctg gacctaccaa tcctcaacat acgatgagta ttgtgacatc
     9781 cttgttgtat agcaggttta atgctggaga gttaccgatt gcgatggttt ctactgataa
     9841 tttctcacag aatggtcaac gttttcaaga tagcattttt atgattgcag aagcttggca
     9901 aaaaaaagga tacgtttctg aaaaatttat tgcttattta aaggataaag aaaaagtgac
     9961 ttttccttgg agtatgattg atagaattac gccaaatcct tcggaaagtg tcaaaatgga
    10021 tttattgaag gcgggtttcg aagacttaga tataattcat actgcgaaaa ataccaatat
    10081 cgctgctttt accaatactg aaaaagtcca ttatctagtc attgaagata gttttccaaa
    10141 tggtcgacct aaactggaag cggctggtgt gattatgaca gatagagaaa cagttgataa
    10201 agcagatatc atgaaggtta cgacatgttt aaatccgctc catacggcat tagcaataac
    10261 aggttgtttg ttggggtata aatcaattca ggcagaaatg caagatgatg atttggttgg
    10321 cttaattaaa gggataggtt atgtagaagg cttaccagta gttgaaagtc caggcgttat
    10381 tgatccaaaa caatttattg atgaagtaat caataaacgt ttgcctaatc cctatattcc
    10441 agatacaccg caacgaattg caacagatac ttctcaaaaa gtagcgattc gctatggtga
    10501 aacaattaaa aagtatgtcg aaaaagacgg aaatgcagaa aatctaacat ttattcctat
    10561 cgctattgct gcttggttac gttatttatt ggcagtcgat gatcaaggaa aaaattttgt
    10621 gccaagtcct gatcctttat tggtagagtt gcaggaaaaa ttctcagtat tgaaattagg
    10681 tgaacaagat cctgcaaaga ttcattgtgc ggtacaggaa atcttagaaa ataaggaaat
    10741 atttggttta gatttatatc aagttgagat a
//
LOCUS       DACEDM010000089        10767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEDM010000089 DACEDM010000000
VERSION     DACEDM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.090"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            79..1125
                     /locus_tag="IWO50_002487"
     CDS             79..1125
                     /locus_tag="IWO50_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0359576.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IWO50_002488"
     CDS             1474..2106
                     /locus_tag="IWO50_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0359577.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IWO50_002489"
     CDS             2694..4133
                     /locus_tag="IWO50_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0359578.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IWO50_002490"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IWO50_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IWO50_002491"
     CDS             4895..5110
                     /locus_tag="IWO50_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359579.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IWO50_002492"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IWO50_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IWO50_002493"
     CDS             complement(5770..6681)
                     /locus_tag="IWO50_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0359580.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IWO50_002494"
     CDS             complement(6700..7704)
                     /locus_tag="IWO50_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0359581.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IWO50_002495"
     CDS             complement(7701..9824)
                     /locus_tag="IWO50_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0359582.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..10749)
                     /locus_tag="IWO50_002496"
     CDS             complement(9829..10749)
                     /locus_tag="IWO50_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0359583.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3293 a         2032 c         2018 g         3424 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccacc
//
LOCUS       DACEDM010000090         9552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEDM010000090 DACEDM010000000
VERSION     DACEDM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.091"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(121..789)
                     /locus_tag="IWO50_002497"
     CDS             complement(121..789)
                     /locus_tag="IWO50_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0359584.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            923..1210
                     /locus_tag="IWO50_002498"
     CDS             923..1210
                     /locus_tag="IWO50_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359585.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1626..3164
                     /locus_tag="IWO50_002499"
     CDS             1626..3164
                     /locus_tag="IWO50_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0359586.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3161..4126
                     /locus_tag="IWO50_002500"
     CDS             3161..4126
                     /locus_tag="IWO50_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359587.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4113..5435
                     /locus_tag="IWO50_002501"
     CDS             4113..5435
                     /locus_tag="IWO50_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0359588.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5498..5602
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5676..6419
                     /locus_tag="IWO50_002502"
     CDS             5676..6419
                     /locus_tag="IWO50_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0359589.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6434..7426
                     /locus_tag="IWO50_002503"
     CDS             6434..7426
                     /locus_tag="IWO50_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0359590.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7438..8175
                     /locus_tag="IWO50_002504"
     CDS             7438..8175
                     /locus_tag="IWO50_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359591.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8165..8887
                     /locus_tag="IWO50_002505"
     CDS             8165..8887
                     /locus_tag="IWO50_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0359592.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8971..>9552)
                     /locus_tag="IWO50_002506"
     CDS             complement(8971..>9552)
                     /locus_tag="IWO50_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0359593.1"
                     /translation="AGAAFFMGTTAHADEAYTVQSGDTLSTISQKYVGDNSLINAIAE
                     SNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3126 a         1641 c         2014 g         2771 t
ORIGIN      
        1 tttcgcagat cgtttgcaaa aatagtttat ttttttataa agaagatttt actgaataat
       61 atcattttag tttacataat gatatttctg taaattaaat aaagtagtga aaaataagaa
      121 tcagtgctct aactgttcaa gaggatcaag tggttcaaat gtttctgtag tctgttttgc
      181 ttccaattgg ggcaaaacag atccaatttc cagatacttc actttattca atgtataaga
      241 tagaatagaa cagctaagta atgtatacag aaaatgtgtg agatccaaat aaagcaagga
      301 gacaactaaa ataccaaaag taatcaagaa aagacttttg ccaatcttaa tatgaaaata
      361 tttttcaatg gcttgataaa tgatatctgt tccgcctgtg cttccgccga atcgaaagac
      421 gagaccgcaa ccaatgccag aaagaatacc tgtcatcaaa ctattgatca gtagactatc
      481 tacagcaaaa tgtatatgaa ggctctccca taaatgtaaa aagaaaacta gcgcaaatgt
      541 tccgacgatc gtccggagaa aagcgtgttt tccaaaaaga aacaatgaga taagcaataa
      601 aggtaaatta aaaagcagtg aagtgacaga aggagcgaat ccaaaaagat tatgcaaaag
      661 caaagcaata ccagtaaccc caccatctgc cagcttagat ggaatattga ttcctgtcac
      721 agctaatccg tagattcctg cgcctaaaat gattccgcac aattctgtaa ataaatttaa
      781 ttttttcatt tctagcctct tttcttttga aaattttcag caacttcttt attatactga
      841 cttatactct tacttcaaga ggaaaatgaa aaaaaattat ttttggctgt ttctttttat
      901 ttatcaaata aggagtggaa aaatgcgagc agcagactta ttgacaattc ttacaaaatc
      961 acccaaaaaa acagagtatc aaggagtttt tttggctcaa gaacaaaaac tggtgcctgt
     1021 gacaatggtc cagataaata aagcaaatca atttatcttt ttatttgaac acggcaaagc
     1081 accacttgct atgaaagaca tctttctttt tttgatgaag aaccgtgaga aagctgttta
     1141 ttatcagtca aaagaagaac tcattcctct ttatggagtg aaagagatag aaaataaagt
     1201 ggtgatataa gtaaaaataa gaaaacaaga cgtttatgat gtcaaaacaa ccatttttta
     1261 catttttggc aaaaaagtgt catgatacag tataatattc gttgaacagg ccaataaagg
     1321 tgttctcccc cacgagattt ctactttatt ggctgtttct ttttattaaa ataaaagttg
     1381 tgtgtgtaaa aaagatatag ctgattccaa gtgacaattt cgtatagatt tttatggtat
     1441 tcgtagtgaa aaaacgagaa ccaaaaagtt gatccgactt tgttgcttgg attctttttt
     1501 tgtcagcata tttagaggta tttttttctt ttttttgttc gtgctgtaag gttaaggcaa
     1561 gacaagttag ttgggaaaaa ggaaagaaat gtttatactt aaagaataac agaaaggaag
     1621 gtcgcatgaa taattttata caaacattaa gtgaaagaaa aggcgagttg ctggaagctg
     1681 catttcaaca tctgtccatt tctctcattt ctttgctgat tgcagcactg attgctattc
     1741 ctttagcgat ttgggcagcc aatcataaaa aaatggcaga agtcttattg cagatcacgg
     1801 gtgtgctcca aacgattcct tcacttgcct tattaggttt acttatccca tttgtaggga
     1861 tcggaactgt tccagcattg attgcacttg ttatttacgc tttgcttccg attttccaaa
     1921 acacgtatat tggtctagct gaaattgacc cgtctattga agaagcagcg gtagcatttg
     1981 gtatgtccag aatgagacgc ttgctgaaag tagagcttcc catcgcatta cctgtcatta
     2041 tctcaggtat ccgaactgcc ttagttttaa tcatcggtac ggctactttg gcagcattga
     2101 tcggagcagg tggattaggg acatttattt tgttaggtat tgaccgaaat acacctgttt
     2161 tgacattgat tggtgcgatc agttctgcct tgcttgcaat tatattcagc ggactgattc
     2221 gctggttgca gcataagaaa cctcgatatt ccatcattac tttagcagca gtggtgtgtt
     2281 tatttggcgg cgtttcttta tatcagtctg ccttgtttaa aaatgaaacc atcacgattg
     2341 ctggaaaatt aggagcggaa cctgaaatcc tgatccaaat gtataaagaa ttgattgaag
     2401 acaacacaaa aacagaagtt gaattaaaac cgaattttgg caagacgagt tttttgttca
     2461 atgcactgga tcaaggtcaa atcgatatct atcctgagtt cacaggtact gtcctagaaa
     2521 gcttggtgaa ggtacctgaa gcaatgaaaa gtgctgattt gacaaaagaa aaaacatatg
     2581 acgaagcaga agaattattg aataagcaat tcgatatgac attattaaaa ccaatggctt
     2641 accaaaatac gtatgccttg gcagtcaaga gatcttttgc gaacgaacat caacttaaaa
     2701 agatttccga tttggcagct atctctgatc agatcaaagc gggattcaca ctggaattta
     2761 ttgatcgttc agatggatat aaaggaatcc aagaagcata tggcttgaat ttttcaagtg
     2821 tacaaagcat ggagccaagt ttgcgttacc aagcaatcaa taatggagat gtcaatattg
     2881 tggatgccta ttccacagat agccagctca aggagtacga tctagtgact ctagaagatg
     2941 atcgagggtt attcccagct tatcaaggcg caccgctgat gaaaaaagat tttgctaaag
     3001 aacatcctga aatcgttaaa gcactgaata aattaagcgg aaaaataaca gaggatcaaa
     3061 tgattgatat gaattaccaa gtcaatgtcg aaaagaaaaa gccgacagaa gtagcacgaa
     3121 tcttcctgaa aaatgaagga ttagtaaagg aggaagatca gtgactaaat taatcgagtt
     3181 caaacatgtg caaaaaaagt atgatggaaa gtatgtcatc gatgacctaa atctggcaat
     3241 caaaaaagga gaaatctttg ttttggttgg tccttccggt agtggaaaaa caactacttt
     3301 gaaaatgatc aatggattat ctaaaccatc agcaggagac atctacttca aaggaaaatc
     3361 gttaaatgaa tataatctgc agaaaatgcg gtggaatatg ggatatgtgc tgcagcaaat
     3421 cgctttgttc ccgaccatga cagtcaagca aaatattgaa gtcatccctg aaatgttagg
     3481 ttgggaaaag cagaaaagag cagaccgtgt agatgaactt cttcaaaaag ttggcttatc
     3541 tcctgatatt tatcgagatc gtatgcctag agagttgtct ggaggagaac agcaacggat
     3601 cgggatcatc cgagctattg ctgcaagccc agatgtgatt ttgatggacg agccatttag
     3661 cgcattagat ccgatttcaa gaaactcatt acaagaattg gttttgtcct tacacgaaga
     3721 attgggcacg accatcgttt tcgttacgca caatatggaa gaagcaatca agctaggtga
     3781 tcgtattgca tttatgaagg atggagaaat tatccaatgt gacacaccag agcagttatt
     3841 gatgaatcct aaaaacgact acgttcggca tttctttgat gaaccgaaac agacgaaaga
     3901 gtggcgagta gaagatttag tcgtcaatgg atatttctta aatgaaatac cagaaaatgc
     3961 tcgtcaacaa gtttgctttg atacgcctat gaaagaagta tattcgcttc tttcggtgta
     4021 tccaagcatt gtgatagtag aaaagcaaag aagtattggg tcgctgacaa gtaaagaaat
     4081 ctttgctttt ttaagtcgag aggaggaagg aaatgaaaac atatgatgca atcatcatcg
     4141 gttcaggtgt gagcggctta agtgctgctt atggattgaa agaagcaggt aaaacagtac
     4201 tagtggtcga agaagatcta tggggcggga cttgtcctaa ccgtggatgt gatcctaaaa
     4261 aagtcttgtt aagtgcagta gaggcacgta atcgggtaaa acaattgagt ggaaaaggtt
     4321 ttaacgagat cccaactgcc aactgggaag aacttcaaaa attcaaacga acatttacgg
     4381 atccagtccc agaaagccga aaaaaacaat tagcagaagc tgaaatcgat catctttcag
     4441 gaaccgctcg ttttttggat gattcatcta ttgaagtcaa cgaagaagtc ttccatgcgg
     4501 attacttagt tttggcaacc gggcaaagac ctacgatctt accagtggag ggaaaggaat
     4561 acttgaaaac tagtgcagac tttttatcat tgcctgtact gcctaaagaa atcattttta
     4621 tcggtggtgg ttatattgct tttgagctag ctacgattgc caatgctgca gggagcaaag
     4681 taaccattgt ccatcataat caacggccat taaaagaatt tgaggcgtct ttggtagagg
     4741 aagccgtcca tcaaatggaa gcatctggca ttcagtttgc ttttggtgtt gaaacccaaa
     4801 agatcatttc agaaggtact cgctatcgtt tagtcggaaa agagacggaa ttagtagcag
     4861 atatgatttt ttgtgcgact gggagacagc cgaatacaga atctttagca cttgagcaag
     4921 cgaatatcgt ctttgataaa cacggaattg cagtcaatga ttatctgcaa acgagcaatc
     4981 caaaaatttt tgcgtgtgga gatattgttt caaggaaaac cccaaaattg acaccagtcg
     5041 ccacttttga aggaaattat gtagctaagc ggataacgga tgcaacaagc gaacctatca
     5101 aatatccgat tatacctacg atcgtctacg ccagtcctaa acttgctgaa gtaggagtta
     5161 caaaaagtca cgcctcttcg tcagatcaag tggtagagat ggatcttacc agttggttca
     5221 cctatcatcg tgtcaatgaa ccagttgcta aggctgaatt gacctttgac cagcaaaatt
     5281 atttgattgg tgctgcagtg attagcgagc aagcagacga attgatcgat gatttgacac
     5341 tcgtcatcaa tcaaaaattg actaaaaaag agttggatag ctatatcatg gggtatccga
     5401 cattggcaag tgatctctcc tatttattga aataatcagc atgataacta aagcgcttgt
     5461 tgctttttcc ttcttgtgct acactttaaa aaatgaatac ctgtttctgg ggatgtcgta
     5521 gacgacatga gagtaagttg tgaacttaga cccattgaac ctgtcagtta atactggtga
     5581 agggagaaac atagcagacg cttgaagcgg gttactatgt cgtgcccatt tcaagcgttt
     5641 tttgttattc atattagaag aaaggaggta acaaaatgaa gaaaaatctt tatccagcag
     5701 tcagtatggc attcatttta attatttggc agattgttgt catagtttgg aaaatacctg
     5761 cctttatttt acccggacct gctgctgtat tttctgcatt agcggcagat gcacaagtat
     5821 tactttccca ttctgtcacg acattaaatg aagcagtgct tggtttggta atagctagcg
     5881 ttctttcttt tattactgcc ttggcaatgg attattggaa gtttttagaa atgtcttttt
     5941 atccgttact agtgatttca caaatgttgc caatcatggt gttagggcca ttacttaccc
     6001 tatggtttgg tttcggtatg cttccaaaag tgatccttgt tgtcttgata agctattttc
     6061 cgatcgtagt ttctttttcg gatacattga ggaaagtatc aaaagagcaa atcatctttt
     6121 tgaaaacgat gggggcagat acagggaaga tttatcgaat atataaaata ccagcgggtt
     6181 tatccggctt cttttccggt ttaaaagtgg ctgctactta ttgtgtcagc ggagcagtcg
     6241 ttggagaatg gctaagcgct caagcaggct tagggtatta catgatacgc gtaaaaaaca
     6301 gctaccagat cgataaagtt tttgctgcga tcatttgtgt cattctattg agtttattgc
     6361 tgaatggcgc ttgttcatta ttgaaaaaag ggtattatcg tatgctttat attttttaag
     6421 agaaggggat caaatgaaaa aattatggct tttattaccg ctactgttgc ttttatccgc
     6481 ttgtggcact gcaaaagaaa caagtagcaa acaagaaata aaggatttga aaaaagtgac
     6541 acttgttttg gattatgtac cgaatactaa tcatactggc atctacctag caaaagaaaa
     6601 aggctattat aaagaggctg gtttaaatgt ccaaatcatc gaaccaggag ataattcgac
     6661 aagtattggt ctagtaggtg cggacaaagc acaatttggt gtaagttatc aagaagatgt
     6721 cacttatgct cacgcagatg gacagaatat accagtcaaa gcaattgcta cagtgatcaa
     6781 acataatact tctggattcg ccaccctatc agacagtaac atccattcac caaaagattt
     6841 tgaaggaaaa acatatgcag gttggcaaag tccaagtgaa gaagctgtct taaaagctgt
     6901 gatggaaaaa gacggcgggg acttttcaaa actgacaatg gtaggaagca atggagaagg
     6961 gccagaaagc ttaggaaaat cctcagatat ccaatggtac tttgaaggtt gggatatgat
     7021 caaagcaaaa gaagcgggta ttgaactaaa ctatatccca ttgaaagaat tagatgaacg
     7081 gttagattac tatactccgg taattattac gaatgatcaa ctgatcaaaa gtgatccaga
     7141 attggtacag tcttttatgg atgccacgaa aaaaggctat caggaagcta ttaaggatcc
     7201 aaatgacagt gctaaactgt tgcaaaaata tgcaaaagaa aatgatcgga cgtttttaga
     7261 agaatcacaa gcattcttgt caaaaaatta tacggatgat cctgagaatt ggggattgat
     7321 ggaagaaaaa gtttggcgca attatacagc ttttatgcag gaaaacggat tgatcaagca
     7381 agatgtacct tcgcaagaac tattcactaa tcagtttata aaataggagt gatagatatg
     7441 tcaatccaaa tcagccactt gaatgtagtc attgaaaacc tgaaagtatt agaagacatc
     7501 catttcacta tccctaaaga tacctttaca tcgattgtcg cgccaagcgg agccggtaag
     7561 tcaacattgc tgaaaacttt aacaggagtc cagcctatga cttctggaac gatcaaagta
     7621 gacgatcaaa aagtaactgg tctccataca gcatttagtt atatgccgca agaagatatg
     7681 ttacttcctt ggctgagcgt ctatcaaaac gttacgttat atcaaaaaat caatcatcta
     7741 acaatcaatg aagaacaagt aagggattat ctggatatct ttgggttaaa cggctatgaa
     7801 cattttttgc cggaacaatt atctggcggg atgaagcagc gaacggcctt gttacgaaca
     7861 atcatgaatc cttcttctta cttgcttctt gatgaaccat ttggagcgtt agatgctatg
     7921 accagagggc agatgcaaga ctggatgttg aagctgccta aaaaagcaaa gcggactact
     7981 ttacttgtga cgcatgatat cgaagaagca atttatttat cagacaggat tcttgtgctt
     8041 tccgcccgtc cggcacatgt gattgctgag attcaagtgc ctgaaaaacg acggtcacgt
     8101 gaatggctac tgcagcaatc agagttgaag caaacgattt atcagttatt ggcaggtgaa
     8161 ttagatgtta agtgatgctc attgtcattt agatgggagt cagagtttag cactccttca
     8221 gcaggaacac acgattttga cgatcatcaa ttgtgattcg ccagaagaat ggaaggaaaa
     8281 cagacagctt gcagcaagta agactcaagc cttaagttac ggtatccatc cttggaaagc
     8341 agatagctat acgtttgaac aagttgagcc ttttttgaaa aaagcaagga tcatcggtga
     8401 aatagggtta gataatattt ggacgaatgt accgatgact acacaaaaaa aagtatttga
     8461 gcgacagctt gcttttgcag ccataaatga gaaacctgtt gtactgcaca caaaaggatg
     8521 cgaaaaagag atcttaaatt atatccaagc ttaccctaat cactatttga tccattggta
     8581 ttcttctttg gactatcaac aagactatat tgatttaggc tgttatttca gcattggtct
     8641 tgatttgaaa aagaatccag cagtctggca actagctaaa gcagtcccaa tcgaccactt
     8701 acttattgaa acggatggac tacatgcagc agagtgggct ttagggggga ttttttcaga
     8761 aaaagattat gtctcactgc ttgaagaaca gttaaagaca atcgctgaga tcaaagagct
     8821 atcagttgaa gaagttaaaa aaaatactta tcaaaacttg gaacgtttca ttagattagg
     8881 taaataaaaa agagatgtga cacaactagt gtcgcacctc tttttggctt tctttgtata
     8941 aaagtaaact gtttgattaa taaatgaatc ttagtaccaa ccgtttgctt cccagaatgc
     9001 tttcgccgct tcccaagaac cataacgtga agtaacatat tgttctgcta ctctttcttg
     9061 gtttgcagca gagtagtcac catttaagta agaagaatct aattggtaac gtccgatgta
     9121 acgtccgttc gttgctgtgt aagaaccgct tgattctttt tgtgcgatcc attcttttgc
     9181 agagcttgtg cttgctggag tagcagcagt ttctgttaca gcaggagcag gttgtgtttc
     9241 agcaacaggc gctgcttgtt tttccgtttg aaccgtttct tgtacaacag gttgttcagc
     9301 ttggactggt gcttcttgaa cggctgcttg aggttcagca gctgcttgtg cagagccttc
     9361 tgttgggatt gttaattgtt gtcctgagta aatcaggtta atatcagaaa ttgaatttga
     9421 ttctgcaatg gcattgatca aggagttatc accaacgtat ttttgagaaa tcgttgatag
     9481 agtatcacct gattgaacgg tataagcttc gtccgcatga gcagtcgttc ccatgaagaa
     9541 tgcagcgccg gc
//
LOCUS       DACEDM010000091         9387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEDM010000091 DACEDM010000000
VERSION     DACEDM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.092"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..77
                     /locus_tag="IWO50_002507"
     tRNA            2..77
                     /locus_tag="IWO50_002507"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            79..149
                     /locus_tag="IWO50_002508"
     tRNA            79..149
                     /locus_tag="IWO50_002508"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Gly,seq:tcc)
     gene            163..236
                     /locus_tag="IWO50_002509"
     tRNA            163..236
                     /locus_tag="IWO50_002509"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ile,seq:gat)
     gene            261..348
                     /locus_tag="IWO50_002510"
     tRNA            261..348
                     /locus_tag="IWO50_002510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:295..297,aa:Ser,seq:gct)
     gene            370..441
                     /locus_tag="IWO50_002511"
     tRNA            370..441
                     /locus_tag="IWO50_002511"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Glu,seq:ttc)
     gene            464..553
                     /locus_tag="IWO50_002512"
     tRNA            464..553
                     /locus_tag="IWO50_002512"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Ser,seq:tga)
     gene            566..639
                     /locus_tag="IWO50_002513"
     tRNA            566..639
                     /locus_tag="IWO50_002513"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:Met,seq:cat)
     gene            658..730
                     /locus_tag="IWO50_002514"
     tRNA            658..730
                     /locus_tag="IWO50_002514"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:Asp,seq:gtc)
     gene            734..806
                     /locus_tag="IWO50_002515"
     tRNA            734..806
                     /locus_tag="IWO50_002515"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:767..769,aa:Phe,seq:gaa)
     gene            811..881
                     /locus_tag="IWO50_002516"
     tRNA            811..881
                     /locus_tag="IWO50_002516"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            894..967
                     /locus_tag="IWO50_002517"
     tRNA            894..967
                     /locus_tag="IWO50_002517"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:928..930,aa:Ile,seq:gat)
     gene            979..1050
                     /locus_tag="IWO50_002518"
     tRNA            979..1050
                     /locus_tag="IWO50_002518"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Glu,seq:ttc)
     gene            1316..2509
                     /locus_tag="IWO50_002519"
     CDS             1316..2509
                     /locus_tag="IWO50_002519"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0359594.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2639..4111
                     /locus_tag="IWO50_002520"
     CDS             2639..4111
                     /locus_tag="IWO50_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0359595.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4194..4982
                     /locus_tag="IWO50_002521"
     CDS             4194..4982
                     /locus_tag="IWO50_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0359596.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5049..5717)
                     /locus_tag="IWO50_002522"
     CDS             complement(5049..5717)
                     /locus_tag="IWO50_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0359597.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5832..7544
                     /locus_tag="IWO50_002523"
     CDS             5832..7544
                     /locus_tag="IWO50_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359598.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            7547..9319
                     /locus_tag="IWO50_002524"
     CDS             7547..9319
                     /locus_tag="IWO50_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359599.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2767 a         1643 c         2121 g         2856 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgccatgc gggtgtagtt tagtggtaaa accacagcct tccaagctgt
      121 tgtcgcgagt tcgattctcg tcacccgctt ttagtttttt atgggcctat agctcagctg
      181 gttagagcgc acgcctgata agcgtgaggt cgatggttcg agtccattta ggcccattag
      241 tacaaaatct tattggtttt ggggaagtac tcaagtggct gaagaggcgc ccctgctaag
      301 ggtgtaggtc gggaaaccgg cgcgagggtt caaatccctc cttctccgtt tcaatatttt
      361 gttttacctg gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg
      421 gttcgaatcc cgtacgggtc attaatttaa tatagtttta catggaggat tacccaagtc
      481 cggctgaagg gaacggtctt gaaaaccgtc aggcgggtaa aaccgtgcaa gggttcgaat
      541 cccttatcct cctttttcat attatcgcgg gatggagcag tctggtagct cgtcgggctc
      601 ataacccgaa ggtcgtaggt tcaaatcctg ctcccgcaat taaaaactaa aaacattggt
      661 tccgtggtgt aggggttaac atgcctgcct gtcacgcagg agatcgcggg ttcgattccc
      721 gtcggaaccg tttggctcgg tagctcagtt ggtagagcaa tggattgaag ctccatgtgt
      781 cggcagttcg attctgtccc gcgccattat gcgggtgtag tttagtggta aaaccacagc
      841 cttccaagct gttgtcgcga gttcgattct cgtcacccgc ttttagtttt tatgggccta
      901 tagctcagct ggttagagcg cacgcctgat aagcgtgagg tcgatggttc gagtccattt
      961 aggcccattt attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg
     1021 gtaacacggg ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt
     1081 tttatctgaa agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct
     1141 aagctttttt ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa
     1201 aaaatacgag cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag
     1261 gatccgcagg acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt
     1321 agaaagacac ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga
     1381 ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc
     1441 gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc
     1501 atatgtagat attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc
     1561 aaaattcgga tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc
     1621 tgatattgct caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt
     1681 attagatgag attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac
     1741 accagaattg atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga
     1801 tttgcgtaaa tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt
     1861 tgaatatgat gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca
     1921 tgatgatgca gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga
     1981 agttattcca gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg
     2041 atttgtcatt ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga
     2101 tacgtatggc ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa
     2161 agttgaccgt tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg
     2221 gcttgctcgc aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc
     2281 gatctcaatc aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt
     2341 gagagaaaac ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc
     2401 gatttacaaa caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg
     2461 ggaacatacg gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag
     2521 ctggaacgat cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa
     2581 aaagatagta attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat
     2641 gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac
     2701 ggctattgaa ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg
     2761 aatggaaatc atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc
     2821 gatttacgga aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt
     2881 attgtttatt atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc
     2941 tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat
     3001 cggcgatctt tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg
     3061 ggggattgcc agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg
     3121 gcactggatc ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct
     3181 atatttaaat gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt
     3241 tagtttgatg gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg
     3301 aatgagtccg cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt
     3361 ttttgccgaa aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact
     3421 atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt
     3481 ttatatccct atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt
     3541 attagcacca ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac
     3601 tcaaatgaaa actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc
     3661 ttggttgtat cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt
     3721 catcggtata ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt
     3781 atctcgtgac cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca
     3841 aacagtcatg gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc
     3901 agcagcaaat ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc
     3961 taaatatatt gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca
     4021 ttccgtttat ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt
     4081 tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat
     4141 cattcaaatt taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat
     4201 ttattcaagc aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac
     4261 taccgcaaac aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac
     4321 ctgttttata tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt
     4381 cgattgaacg ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg
     4441 gctggtacac agatacagtt tatgacatga agtattggac attcattgca gaagagctac
     4501 ctgagatttg tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg
     4561 ctggattatc gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt
     4621 tcgcagcagt aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt
     4681 tgatggttcg taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag
     4741 gttcaaaaaa tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc
     4801 caagatttta tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg
     4861 tatctgctat gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata
     4921 actggatctt ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat
     4981 aaaaaaagag tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag
     5041 gagctttttt actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag
     5101 gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt
     5161 tctttaaatc cctctaattg attttcaact acataattac gcataaatag aactgtttct
     5221 ttcaaaaatt catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc
     5281 attaactgtc caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact
     5341 gaaaatcctt cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga
     5401 aaagcgggca cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca
     5461 tagtaatttt ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc
     5521 gctaattctt cttccattgt ttcagccatt ttttgcaaga gctgttgttt gtttttgaaa
     5581 taccaatata aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc
     5641 tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga
     5701 gaaagcttta tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa
     5761 attgttagtc ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt
     5821 gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta
     5881 tttccttgct gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac
     5941 agcaagtatt ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt
     6001 acctgatcag tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg
     6061 caaaagtagc tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga
     6121 cattttcatt tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa
     6181 atgatatcac gcaaatccaa aatttagtga tgatttcttt gcaatcactt tttcgaattc
     6241 cattcttatt tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga
     6301 tcatcgtagc gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg
     6361 gcaagcactt catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa
     6421 acttactagg gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat
     6481 tcagcaaagt aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg
     6541 tgatgatccc gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg
     6601 tgagcgatct tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact
     6661 acttgatgca gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa
     6721 gagcagccgt atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt
     6781 atttagacgt tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc
     6841 gttatcctgg tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag
     6901 aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc
     6961 ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag
     7021 taaatgaaaa gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct
     7081 caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg
     7141 aacatgcttc ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg
     7201 aagcaccaat agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca
     7261 ttactcgtgg cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac
     7321 tggatgctcg ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa
     7381 cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat
     7441 tggatgaagg cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg
     7501 tctatcagga aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat
     7561 aaaagcaagt aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat
     7621 ctttatcaca gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga
     7681 agcttttcaa gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag
     7741 caaatttgtt tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa
     7801 ctttatctat agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat
     7861 cggtctattc aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg
     7921 tgaaatttta agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc
     7981 tttgcttcaa gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg
     8041 ccaaaatgta gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc
     8101 tgtcttgatt atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa
     8161 actaaatggt tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt
     8221 acaagaagaa acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt
     8281 caaaggacaa gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa
     8341 tacagcgata gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc
     8401 ggtggcatta ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact
     8461 gatgcagatt tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt
     8521 gaatgaaata tttgatgaaa aagatgaagt caaaccagaa aacggtaaag catttgatgg
     8581 tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt
     8641 cttgaaagat gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac
     8701 aggatcgggg aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg
     8761 ttcagtagtc atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca
     8821 tgtaggaatc gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac
     8881 attcggcaaa ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat
     8941 tcatgacttc atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa
     9001 cttattcagt acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc
     9061 atctttattg attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat
     9121 ccaaaaagca atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct
     9181 gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga
     9241 aggcagtcat catgagttat tagcagaaaa tggattttat gcggaacttt atcataatca
     9301 atttgtattt gaaagttaaa ttaaaagaac ctcgttataa cggggttctt tttatatatt
     9361 ttatgttgtt tgtttttata aggggca
//
LOCUS       DACEDM010000092         9159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEDM010000092 DACEDM010000000
VERSION     DACEDM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.093"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWO50_002525"
     CDS             6..305
                     /locus_tag="IWO50_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0359600.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWO50_002526"
     CDS             378..1739
                     /locus_tag="IWO50_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0359601.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWO50_002527"
     CDS             1761..2021
                     /locus_tag="IWO50_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359602.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWO50_002528"
     CDS             2035..2787
                     /locus_tag="IWO50_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0359603.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWO50_002529"
     CDS             complement(2881..3486)
                     /locus_tag="IWO50_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0359604.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWO50_002530"
     CDS             complement(3539..4513)
                     /locus_tag="IWO50_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0359605.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWO50_002531"
     CDS             complement(4891..5670)
                     /locus_tag="IWO50_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0359606.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO50_002532"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO50_002532"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0359607.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWO50_002533"
     CDS             7161..8558
                     /locus_tag="IWO50_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0359608.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWO50_002534"
     CDS             8639..9079
                     /locus_tag="IWO50_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0359609.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
BASE COUNT         2892 a         1645 c         1898 g         2724 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacttgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattac
//
LOCUS       DACEDM010000093         8855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEDM010000093 DACEDM010000000
VERSION     DACEDM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.094"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            62..676
                     /locus_tag="IWO50_002535"
     CDS             62..676
                     /locus_tag="IWO50_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0359610.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(841..1665)
                     /locus_tag="IWO50_002536"
     CDS             complement(841..1665)
                     /locus_tag="IWO50_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359611.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(2303..2560)
                     /locus_tag="IWO50_002537"
     CDS             complement(2303..2560)
                     /locus_tag="IWO50_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0359612.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2553..2822)
                     /locus_tag="IWO50_002538"
     CDS             complement(2553..2822)
                     /locus_tag="IWO50_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0359613.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(2953..3132)
                     /locus_tag="IWO50_002539"
     CDS             complement(2953..3132)
                     /locus_tag="IWO50_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359614.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(3513..4220)
                     /locus_tag="IWO50_002540"
     CDS             complement(3513..4220)
                     /locus_tag="IWO50_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359615.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(4213..4650)
                     /locus_tag="IWO50_002541"
     CDS             complement(4213..4650)
                     /locus_tag="IWO50_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359616.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(4665..4916)
                     /locus_tag="IWO50_002542"
     CDS             complement(4665..4916)
                     /locus_tag="IWO50_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359617.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(4916..5122)
                     /locus_tag="IWO50_002543"
     CDS             complement(4916..5122)
                     /locus_tag="IWO50_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0359618.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(5559..5819)
                     /locus_tag="IWO50_002544"
     CDS             complement(5559..5819)
                     /locus_tag="IWO50_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359619.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            5894..6097
                     /locus_tag="IWO50_002545"
     CDS             5894..6097
                     /locus_tag="IWO50_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0359620.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(6238..6723)
                     /gene="ssb"
                     /locus_tag="IWO50_002546"
     CDS             complement(6238..6723)
                     /gene="ssb"
                     /locus_tag="IWO50_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0359621.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(6726..7625)
                     /locus_tag="IWO50_002547"
     CDS             complement(6726..7625)
                     /locus_tag="IWO50_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0359622.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(7638..8231)
                     /locus_tag="IWO50_002548"
     CDS             complement(7638..8231)
                     /locus_tag="IWO50_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ0359623.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(8472..>8855)
                     /locus_tag="IWO50_002549"
     CDS             complement(8472..>8855)
                     /locus_tag="IWO50_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0359624.1"
                     /translation="GQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQN
                     VAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKE
                     DFTTGFEFSEKKKTNYKKRTRRTTK"
BASE COUNT         2518 a         1698 c         1380 g         3259 t
ORIGIN      
        1 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
       61 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
      121 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
      181 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
      241 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta
      301 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
      361 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
      421 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
      481 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
      541 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
      601 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
      661 ggtcaatgac aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac
      721 atataattac tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa
      781 caaaccattt tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg
      841 ttaatcccct actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc
      901 ttgtgattta gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat
      961 agctagttct tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt
     1021 atttggttcc gttggagtca attctataga ttcttcataa aaatctctat acataaagct
     1081 atcattaatt ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca
     1141 ttcttctata gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat
     1201 gaaattttta atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa
     1261 catgctccct aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa
     1321 atcaggtttc tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga
     1381 aataaaccta tctctagtat ttgatatatt tttacttctt ttaaggagta taagagaaaa
     1441 ggggatactt tcgtgtccat gcatgtaatt aactaattgt tgagcttcag aagttatatc
     1501 tctaattata tagttaacgg aattaggaag tgtctttaac tctaatgaaa tttctatcca
     1561 attactatct tctaatctgt atatgctcga agtataattt gattcgtcag caagcatcgc
     1621 tacatcccaa acactgatca taaaatcttt taaacgttca atcatttcag caatattgct
     1681 aaaatcagct aatttttctt tagaataaaa aaaacattta tatcagaatc aataaattct
     1741 atttttttta ttactatatc gtcatcatta tcttgaccat ttatgtttat tgttccctga
     1801 gtggggagta cttctttcaa attcaagtta aatttatatt ccattacttc tcctccaata
     1861 tataaaaatt acttaactgt atccaaattt aatatgtaaa taaatcatta atttttagct
     1921 aaattctatg agaagtttat ttttgttgtt tttgctaatt aactaaaact agtacaaaaa
     1981 atttaaaata gattattaaa cctattgctt attctattca gtcatatata taagcgtcat
     2041 cctttagtaa ctaacatccc acactacaat agtattataa catatctatt caacttcaaa
     2101 ttgttgaaat ttaagccatt tatatttact tttcctcaac tttagaaatg attttactaa
     2161 ccttatatcc gtaatacgcg taacaaacac attcattaat aacaatcaac actaagctat
     2221 attagctttt taaatgacta gggtaaataa aataaggatc aaaaactttt aagtttctga
     2281 ccctttcctt acttccgatt ggttaatagt gatcttttgc agaataaata aatatcgttt
     2341 cattttcaac tctatatatc agtctatgtt catctgtaat tcttctggac cattttccag
     2401 ataaatcatg ctttaatggc tcaggttttc ctaatccagc aaagggggaa cgatcgatat
     2461 cttttattaa cttgttaatc ttttttatat tgcttttgtt tccttgctca tgccaataaa
     2521 gataatcatc ccaagcatca tcagaccaag ccttaatcat cagattcaac ctcgattaag
     2581 tcatgtgttt taaatgcacc tttggagaat tgttcatctc ctcgacgaat tttttccatg
     2641 acgtaattat tagaaagtgt tctcaacgtt tcttgcatag aatcataatc tctttttgat
     2701 aatacaacaa ctgtatcttc tacatcttta cttgttacaa taagtgtttc agcatcctca
     2761 ttaacttgtt tcatataact acgtaaattt tggcggaaat ttgaataagc tactgcttcc
     2821 attcctttca ccccttattt atttctataa aacaattgta cattatattg tacaattaag
     2881 caaatgatta atattctaac ttcttataat taaaagtgct atcactttta gtttttagga
     2941 taaaaagtga cgtcactttt tgagtgctgg tacctcaaat cctgcttttt cttgggcaat
     3001 caataaatct aacgcaatac acataatcct ggattcactt ttgccgtact tctgctttag
     3061 ttgctgcaat tctttttgtc tcttttcatc caacgtcacg ggtaaccgtt tatttcctat
     3121 tgcaattggc attagtactc tctcctttca tcgaaataac cctattaaaa atatggctgc
     3181 aatcaacttg aatattagac tcacaagggt taacacaaag cgccaaaaga tcaaacaaaa
     3241 taccagtatt cctaagaata ctacgaagtt taaatgtaga gctgcaccat gacgcataag
     3301 tatctcgcca acaaacagta cgaacaatag aggaatgatt ttattcatat ggatctcctt
     3361 ctttctctta ggttatcggg ctttgcccgc ttatttcgca ccacctaagc ggcgggggcg
     3421 gttggatcga ttggtgcgaa ataagaacca ttcccttaat ttcaaggttt tttgttctta
     3481 tttcacacat cctcactttt cggtataaat ggttattgac cttttttcct ttcaaatcgt
     3541 tgatatggca ttcgtttacg gtcaagttca ttttggattt cttctgtatc gtcgtaagtg
     3601 taggtgtcta aaaaatcagt taaagtcgtg taccagtcgt cgcctgggtt atctacttct
     3661 tctacatatt ccctcaaaaa tgcttcgggt acagtgagtt cataatccat tcccattaac
     3721 acgatagatt ccgtcccaca cgtcaaaaat ctagtaaata caaggtaata atcgtctacc
     3781 gtcgcatctt tgctgtttag ctcttccatt tcttcttcca ttaattccat gctctcaaaa
     3841 attgaataat attctgattc tgagtaatag tgtttcctgc aaatcgtgta gctgacaaag
     3901 ttttcgacca gctcactcct ttctaggtaa acattcatga tatatttttc tgtttctagt
     3961 cgtagaagct ccgctccttt ttttaatgtg tcaggacgac gtttatacac gtctaaacga
     4021 ttatggctga ctgtaacctt cgtttgactc gtttcacttt ccatatacaa ctgttcttcc
     4081 actctttgaa taaagtactt catttcttcc atacaactaa agtctttttg aatccgtctg
     4141 attacttcat tcaagtgttc tttgtttgtt aaaaattgcg aaacatcttc tgttgttagc
     4201 tgataaacca ccttattcat tatctttctc ctccgattca atcgtataaa atgcaccatt
     4261 tttttcgata ttttcaaaag tatctagcca gtcgctagcc attgctttag ctttcttaat
     4321 ctgtgcgaat tttgcataag tcagttctaa ggcttgatat tcggaaggtt tgatagtgct
     4381 tggtttgaca cctacttcca agcatagtaa ggcttggaaa aaccattttc cattttgttt
     4441 tttcagttct tccagtgttc gatagtacgt cgcaatgaga ttcatttcga cacctgattc
     4501 aatcatttct tccactgcga ctgacaattt ttccttgtaa tgttttttgg gtacacgtgt
     4561 ttcatggtat aggaacgtca tatcctccat tttttgtgcc attttatcca aaggtaattt
     4621 gacaaattcg tcatagtaat tttgagccat attttttctc ctttttagtt ctctaatttg
     4681 gttaaccaat attcgatttc ataagtttcc atgttttctt gataatgcag ttcatatcct
     4741 gcaatcttca ctaggtcttc aaatagcata gtcggtgcat cctctactaa aggggtttct
     4801 atcgtccagt accgcggaat aagtaaagct tctgctactt tttcagaaat gacttctttg
     4861 tccaccaaaa tttgaacgac ttcttttaca gtcaataatt tgtgttgtgt tttcatcacc
     4921 gtttctttac tttctcatca atcaaaacat aatcgccata agacaaggta tattttttcc
     4981 cgcatgttgg acattgatac aaacgatagc ttttgataca tttcgctctt tctccacaat
     5041 cgcattcgac agtatgtggt gcatgatagt ttgggaataa ttcgctgaag ggaacacccc
     5101 aataatcaaa tttctttcgc atgattgatt ctgcctcctc ttaggaaagg ttttatttcc
     5161 tttcgttagt tctttcttta ttgatttgtt ggccagaatg aatcaacgaa aagcgacaag
     5221 gacaagggcg cgcagcgtgc catttaccct tgtccttgtc gctttttggt gataaaaggc
     5281 aacttgttgc cggtctggca aatcaataaa gagatagcga acaggaacga accttttggt
     5341 gagaggaagt tttttctatt ttgtcttgct ttgcaagaca ccatatctta gtgaacgtgc
     5401 aacgttcact tcctttcttc tcgaacggcg gtggcggttg gagtgagaga aaggtttttc
     5461 aattgagaag gcacttgtgt cttcctaaaa aggtcttttc ctgtttctat gtaaaacaaa
     5521 aagagctgca atcctcattt aggactgcag ctcttctttt atatgcccat cgatcgcgta
     5581 aaatgatcgg ggtttctaat ttgcatcgat tcaatgacta attgattccg tttattgaaa
     5641 tgacctgtga cggcaatatt gtattttcca tcatccataa ttaacaattt atcgataatt
     5701 tcaatatctg caactacaca attaatcgat tcatgaattg tttgtaaagt aaagcgaacc
     5761 aataatggtt gtgtttttat tactcggatt ttatcaagca aaccaacata tttgttcatc
     5821 acgatcacct cgctaaaatt atagcaaaaa cagctaaaat tgagaatttt tattcgattt
     5881 tagatatgct ataatgatca agacagtaaa gtttgtgacg gtggctatcc gattgaaagt
     5941 gaggtggtgc gtatgtgcgt acttccaaaa tcttacagaa aggagaagcc ttttgcctgt
     6001 tgaaacggcg ctaggattaa tgattagttt cggttctttg atcgcaacaa tcatttttgg
     6061 aatcttagca gttgtaaaca acaatcaaaa aaaataaccg tcagctttag ccggtcgacg
     6121 attatttttc aaaaataata ttaagctggt caccgtctta aacggtgctg ttaggggagt
     6181 tgttagcgca actcctcttt tcgtatccat tataacaact ttattgatct tttacaatca
     6241 gaatggtaaa tcatcatcac taatatcgat tgattgtccg ccaaatggat tttgtgaagc
     6301 gttcaatcca ttgttgttag gcgtagctac atcccgttgt tcaaaggtgg tagggcttgg
     6361 actgctggac gattgatcag cgtgcgcacg gctttctgtc gctgctttac tttctaatag
     6421 ttggaaatta tcggccacaa cttctgtcac atacacacgt tgtccttgtt ggttatcgta
     6481 ggttcgggtt tgaatccgtc caacaacgcc gatcaaactg ccctttttag ctaatgtcgc
     6541 catcgtttct gctggtttgc gccaaatcac acagttaata aaatcagctt ctcgattgcc
     6601 gttttgattc gtgaaatttc ggttgacagc tagggtaaaa gtcgctactg cagtaccact
     6661 cgcggtataa cgtaaatctg gatctttggt taagcgtcct actaaagtta cattattgat
     6721 catctttatc tctccatttc ttgattcatt tgttgtgatc ttttaaactg ccgttttctt
     6781 tcttgatttt caattttgat cacacttttc attaggtgat caatttcgtt gactgtatct
     6841 gtaaatgaaa tggaattcgc aaacgtgtta actgagcgat atttttccca ctgcttttcc
     6901 aaatagtcat ttcctctgat cttatccatt tctctttcat aataaaagga caacggtttt
     6961 gtatccccac gttttgtaaa tgtatttcga acacttttgt ataactcttt cgtatcgata
     7021 ttttctagtt tcggaatggt aaataagtcg tagtaatctt ttgctctcga attatctgtg
     7081 ccaaagctga acgtagcact tagcttttct gcaataattt gttctgttgg ataagctgga
     7141 atctccactt ttttatcttc aaacattaat tggtgatagt gtgtatctac ttcaggataa
     7201 atagaatctc ctgttgtcac gtccatttta aacggtacac gtactttctc taaattagcc
     7261 accaatttaa tttgaaatcc aggataaaaa tcggcttctc ttgtttcctt cattccttga
     7321 atagagaaaa caatgccttc ttttgtgggt tttctagtta aatcattaaa aatgctcttc
     7381 aatttttctt cagttaattt gctatttcta aatgtcgtat cgatatcaat tgttgtcctt
     7441 ctatcgattc catattttga tcctaataaa aagccgcctt tcaaaatgaa gtcatttttg
     7501 tactttgatt cagaaatttg ttcaattaga cgatccatca taaaatgccg ttgcactaac
     7561 tgtgcattta atcctgtact ctttgctaaa tttcttgttt ttgctgcaaa ttgtgtcggt
     7621 gtcatctttt tcctccatca atataagctt tgcatgtggt atttcatttc tttttctaca
     7681 tgtagttttg tcatgtaatc tcttaattta cttggatctc tcaatgggtc ttccatgtac
     7741 tcttttaatg cttctaattt catttcatat tcaaattcat atctcggatt ccaaatatca
     7801 caaagtgtac gctctttatc atacatagta ataggatttc cttgaaagga agttccctct
     7861 acttttccta gttcataaac ttctttcttc acgaaatgaa attgtaactg ctgctctttt
     7921 gtaattgaac gatcaacgtt atagccatgg ggaaaagtta agtgaatgaa tttcggcata
     7981 ttcgtagaaa gctggtaaat atctaaagtc gtttcatgag agaagattcc cctggaaaat
     8041 cgttgctgaa gccaaaataa atcatctatt ccatgaccat cctctacata ataaccacga
     8101 tcaactttga tcaattttcc ttgattcacc aatctttgta cagtggttgc tgcgattcca
     8161 aatttcctgg cttccttcat agataaattt ccatcgcgaa tagccaacaa ttctttcagt
     8221 ttatcttgca tttacttccc ctcccttcgt tacaaataca ctacatcaaa tatatcatag
     8281 tagtttattt gtaacaagat taacgtctat cgatctaatt cgatttcttt ttgaatgcgc
     8341 tgctggttct ttaattgttg cagtcttctt ttatttgcac gtaatttctc ttgttctaaa
     8401 cgaatttttg ctttttgatt ttcgatttct tgttgtttgt gtgttgtatt tcgcatagga
     8461 taaccccttc attattttgt agtgcggcgt gttcttttct tatagttcgt ttttttcttt
     8521 tcagaaaatt caaagcctgt tgtaaagtct tcttttaaaa cgatcgttgc ttcaaacgaa
     8581 cctttctgac cttttagtcc tctgactgat tggcttgttc ggccgttttg aatgatctct
     8641 ttcagttgtg tggctgtgag tttctttttg gcaatcgttg tccataattt aaaatcacag
     8701 gcttcagacg tacatgccgc cacattttga taaagcaaga cttcttgttc ccgacatttt
     8761 ggacaaacac ctaaatgacg cgttgcttcc tgtttttcac tggctgcttt cacctcttgt
     8821 aacgttgatc caaaatctgt tgactggatt tgtcc
//
LOCUS       DACEDM010000094         8290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEDM010000094 DACEDM010000000
VERSION     DACEDM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.095"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1511)
                     /locus_tag="IWO50_002550"
     CDS             complement(45..1511)
                     /locus_tag="IWO50_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0359625.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IWO50_002551"
     CDS             1898..2494
                     /locus_tag="IWO50_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0359626.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IWO50_002552"
     CDS             complement(2552..3034)
                     /locus_tag="IWO50_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359627.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IWO50_002553"
     CDS             complement(3140..3337)
                     /locus_tag="IWO50_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359628.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IWO50_002554"
     CDS             3632..4390
                     /locus_tag="IWO50_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0359629.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IWO50_002555"
     CDS             4450..4878
                     /locus_tag="IWO50_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359630.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IWO50_002556"
     tRNA            4924..4996
                     /locus_tag="IWO50_002556"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IWO50_002557"
     CDS             complement(5146..5517)
                     /locus_tag="IWO50_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0359631.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IWO50_002558"
     CDS             5732..5917
                     /locus_tag="IWO50_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359632.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IWO50_002559"
     CDS             complement(6000..6188)
                     /locus_tag="IWO50_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359633.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IWO50_002560"
     CDS             complement(6212..6475)
                     /locus_tag="IWO50_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359634.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IWO50_002561"
     CDS             6633..6890
                     /locus_tag="IWO50_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0359635.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IWO50_002562"
     CDS             complement(6961..7152)
                     /locus_tag="IWO50_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359636.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IWO50_002563"
     CDS             complement(7293..7613)
                     /locus_tag="IWO50_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359637.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IWO50_002564"
     CDS             complement(7636..8070)
                     /locus_tag="IWO50_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0359638.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2628 a         1635 c         1314 g         2713 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg
//
LOCUS       DACEDM010000095         8229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEDM010000095 DACEDM010000000
VERSION     DACEDM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.096"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IWO50_002565"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IWO50_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0359639.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IWO50_002566"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IWO50_002566"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0359640.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IWO50_002567"
     CDS             2591..3376
                     /locus_tag="IWO50_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0359641.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IWO50_002568"
     CDS             3389..4240
                     /locus_tag="IWO50_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0359642.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IWO50_002569"
     CDS             complement(4364..4816)
                     /locus_tag="IWO50_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0359643.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IWO50_002570"
     CDS             complement(5281..5649)
                     /locus_tag="IWO50_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0359644.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IWO50_002571"
     CDS             5799..6281
                     /locus_tag="IWO50_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0359645.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IWO50_002572"
     CDS             6318..7328
                     /locus_tag="IWO50_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0359646.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IWO50_002573"
     CDS             complement(7443..8156)
                     /locus_tag="IWO50_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359647.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2441 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DACEDM010000096         7837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEDM010000096 DACEDM010000000
VERSION     DACEDM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.098"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..813
                     /locus_tag="IWO50_002574"
     CDS             <1..813
                     /locus_tag="IWO50_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ0359648.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IWO50_002575"
     CDS             807..1229
                     /locus_tag="IWO50_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359649.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="IWO50_002576"
     CDS             2399..3367
                     /locus_tag="IWO50_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0359650.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="IWO50_002577"
     CDS             3384..3908
                     /locus_tag="IWO50_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0359651.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4084..4488
                     /locus_tag="IWO50_002578"
     CDS             4084..4488
                     /locus_tag="IWO50_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0359652.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            4505..5653
                     /gene="tnpB"
                     /locus_tag="IWO50_002579"
     CDS             4505..5653
                     /gene="tnpB"
                     /locus_tag="IWO50_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0359653.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            6175..6456
                     /locus_tag="IWO50_002580"
     CDS             6175..6456
                     /locus_tag="IWO50_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359654.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            6458..6874
                     /locus_tag="IWO50_002581"
     CDS             6458..6874
                     /locus_tag="IWO50_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359655.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            7496..>7837
                     /locus_tag="IWO50_002582"
     CDS             7496..>7837
                     /locus_tag="IWO50_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ0359656.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2944 a         1321 c         1304 g         2268 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aacagaatta ggcagaagtc gcccatcctc tataggtggg
     3961 tgatgaatgc cgttcggtat gagggttacc gttggtaact cgaagaccga caactaaggc
     4021 gtattgcctt tttattttgt cccatgttac aatcagtcta tacaagaaat gagttgatat
     4081 aacatggaat tagatacaaa taatcattca gtatttcttc tttattacca ccttgtttta
     4141 gtaacgaaat atcgtagaca agtgattgat gatgaaatat ctgattatgc caaaactact
     4201 tttgaaagaa tttcagaatc gtatcacatt actttagtgg agtggaatca tgataaagac
     4261 cacgttcata ttatgttcaa agctcaacct aaaacagaat tgacaaaatt catcaatgcc
     4321 tataaaagtg ccagctcaag attgataaaa cgtgactttc caagagtcaa acaatttctt
     4381 tggaaagaaa tgttctggtc taaaagtttt tgtcttctga caactggtgg cgcaccaatt
     4441 gatgtgatta agaagtatat ccaaaatcaa ggaaacaatc ataaatagaa agtaggtgaa
     4501 ctttatggaa caactaaaag catacaaatt cagaatctat ccaacagagg aacaagaaat
     4561 cttctttgcc aaatcttttg gttgtgtccg taaggtctac aatctaatgc ttgatgaccg
     4621 aaaaaaggcg tatgaagaag ttaaaaatga ttcttctaaa aaaatgactt ttccgacacc
     4681 agctaaatac aagaaagaat ttccgttttt gaaagaaatt gatagccttg ctttagccaa
     4741 tgcccaactt aatttagata aagcatacaa gaattttttt cgggataaat ccgttggatt
     4801 tccacgtttc aaaagtaaga aaaatcctgt tcagagttat acaacgaata atcaaaatgg
     4861 tacagttgct ttgattgata gtaaattcat caaagttcct aaattgaagt cattagtaag
     4921 aattaagctt catagacaac caaaagggat tatcaaatct gctacaatat cacgtcattc
     4981 tagcggtaaa tactatattt ctttgttgtg taaagaagaa attatcgaat tacctaaaac
     5041 taattccaca attggtattg acttaggtat tatggacttt gcgattcttt ctgatggaca
     5101 aaaaattgat aataataaat tcacgtccaa aatggaaaag aaactaaagc gtgaacaacg
     5161 caaattgtct aggcgtgctt tattggctaa aaataaaggt accaatctat ttgaagctaa
     5221 aaattatcaa aaacaaaaac gcaaagtagc tagattgcat gaaaaagtaa tgaatcaacg
     5281 tactgatttt ctgaataagt tgagtacaga aatgatcaaa aatcacgata ttatctgtat
     5341 tgaagactta aacacaaaag gtatgttgcg aaatcataaa ttagctaaaa gtatttctga
     5401 tgtttcttgg tcaaagtttg tgactaaatt acaatacaag gctgactggt atggacgaaa
     5461 aatcatcaaa gtagataaat ggtttccgtc tagtcagatt tgttcagagt gcggacataa
     5521 agatggcaag aaatctcttg aaattcgaga atggacttgt cctgtttgtc atactcatca
     5581 tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa ggtctaagac tacattctat
     5641 gggattggct tagatccaat catcaagaac cgtaggaact acggggatag cttggtaaat
     5701 aagagacacc gctgttagta aagaaatacg ctatcaagta tgctctattc ccaagaagct
     5761 cccacttcaa gcgttaagaa ccgtcaggtt tagctaagtg gtgagtagtt cacattaata
     5821 aaaattgctg gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt
     5881 gcgatatttt ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact
     5941 cgttttacag ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc
     6001 ttacgaaagg tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca
     6061 cttttttgcc attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat
     6121 actatatttg tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac
     6181 attttagttc gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa
     6241 ctcacaacca aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa
     6301 agtgattttg accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa
     6361 aaatttattg ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac
     6421 aaccaactca ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa
     6481 acacgtaacc ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc
     6541 agacgtcaac aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac
     6601 caatacaacc acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat
     6661 catgacagaa atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat
     6721 cggtacaaat tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc
     6781 ttcccgacga aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg
     6841 aaattttgac ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat
     6901 tcctcgattt ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg
     6961 cgaaaaaaaa gaaaaatccg cttatgcaac ttcttctttt tgtattttga ggcgctaccc
     7021 caatccccaa gtatcggtta tttaatagcg cggaaatcca aagaatagga cgtaccctga
     7081 aacaaaaaaa gactgctctc acgagcagcc caaaatgaga ttatcctatt catcaacata
     7141 aaaatattac aaagtgaaga actagagaaa gacaaaaaaa gcatatcaaa tcctctcaaa
     7201 aataacaagt ctttaaaaaa tgacagataa aaatgcacaa agaactaaaa aagagaaatc
     7261 gcgtaaaatc aagcattaga agctagtaac tagctagacc aattaaaaat cacaaagtga
     7321 gcgtctctat ccattggtag aggggctact tggcatagtc gacattatgt taactaacgc
     7381 tataaataac gattatgttc ccaaaacaat attttatgaa ctataacaaa aatttgttat
     7441 cgcatattgt atattttaat tataggtgta taccacttaa atttaacaca aaggcgtgat
     7501 tattttgaaa cccaaaaaga aaatttatct aggtctcctc ttttccttga tgattggaat
     7561 ttttagtata ggaattacaa atacctatgc ttcagaaaat tctaatttca atatcaactc
     7621 ccaaccaatt gaagaacaag caattaattt tgcgaaaact gattttgaac actccataac
     7681 ttcaattctt ttaatggaaa ataacaccga aactgatatt aactatactc ttggttcgcc
     7741 atttaaaatg ctcgatggag aaaatacggc aaatagttct attttctttc cagtattttc
     7801 agacgctgat aatacgatta aatatattta taccgtc
//
LOCUS       DACEDM010000097         7794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEDM010000097 DACEDM010000000
VERSION     DACEDM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.099"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(192..1484)
                     /locus_tag="IWO50_002583"
     CDS             complement(192..1484)
                     /locus_tag="IWO50_002583"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0359657.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            complement(2264..3631)
                     /gene="dnaB"
                     /locus_tag="IWO50_002584"
     CDS             complement(2264..3631)
                     /gene="dnaB"
                     /locus_tag="IWO50_002584"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0359658.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            complement(3905..4357)
                     /gene="rplI"
                     /locus_tag="IWO50_002585"
     CDS             complement(3905..4357)
                     /gene="rplI"
                     /locus_tag="IWO50_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0359659.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            complement(4363..6336)
                     /locus_tag="IWO50_002586"
     CDS             complement(4363..6336)
                     /locus_tag="IWO50_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0359660.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            complement(6479..6715)
                     /gene="rpsR"
                     /locus_tag="IWO50_002587"
     CDS             complement(6479..6715)
                     /gene="rpsR"
                     /locus_tag="IWO50_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0359661.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            complement(6741..7253)
                     /gene="ssb"
                     /locus_tag="IWO50_002588"
     CDS             complement(6741..7253)
                     /gene="ssb"
                     /locus_tag="IWO50_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0359662.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            complement(7303..7602)
                     /locus_tag="IWO50_002589"
     CDS             complement(7303..7602)
                     /locus_tag="IWO50_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0359663.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
BASE COUNT         2156 a         1651 c         1398 g         2589 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga actttatctt tttagaatgg
     1621 ttattaaaaa agaggttgta acagaagtgg tcagcttcaa gaaataagaa ggaatgtacg
     1681 aaaattgctt ctcaaatttt tgtgaaattt cgacttattt ttcgaagaag ctacttttgg
     1741 aacaccgtga atcagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa
     1801 acaaaaatag cttctcatat ttttcgtttg attgggcgta ttaccgcaga tgcaagcttt
     1861 tgaacaccgt ttaagaaaga ggttgtgagc ctgccgttta actctgagtc acaaggagca
     1921 atttcaaaaa actgcttctc atgtttgatg atattgtgac ttgtgaccga ggagttggca
     1981 ggagaacacc gtttaaggaa aagaggtcgt aagatcagcg ttttgacctg agtattagag
     2041 aaacaaacgt caaaatagtt cctcatattt tgacgtttgc tgagctaata ccaagggtca
     2101 gctgattgaa caccgtttat tcggaaaaag ggactgtgac aagacttttg tcacaatccc
     2161 tttggagatc tgtttaaatt aagtactcta gtcgttcctg cggttactcc ttgcatcttc
     2221 ttacgagagc tctcgggatt gctcaaggct cacaacctct tctttaaaat tcttctcttg
     2281 gcgaaagcga cgaaaactta ttgtattctt tgataaacag tagttcaact gttcctcgtg
     2341 caccgcttcg gttcttctcg atgatgactt cgatgacgtt gtcatttttt ggctcgtttc
     2401 catcatcgtc atctccgcct tctctttcat aataatcatc tcgatacaag aaggctacga
     2461 tgtccgcatc ttgttcaatt gatccggatt cacgaatatc acttagcact ggtcgtttgt
     2521 cttgtcgctg ttctactcca cgagataact gtgaaagagc aatgacaggt actttcagct
     2581 ctttcgccag ttttttcagt tggcgagaga tatctgatac ttcttgttga cggttctctt
     2641 taccgttacc ttcaatcaac tgcaaataat cgatcaatat caagcctaga ttgccttttt
     2701 cttgcgctag tttccgacat ttcgcacgga tttcagagat tttgatccct ggtgtatcat
     2761 cgataaaaat atttgctcga gaaagactcc ccatcgcaac gatcagattc gaccattctt
     2821 cttccgaaag ctgacctgta cgtaaatggc tggcctcaat cgaaccttct gcacaaagca
     2881 tccgatttac aagagattct gcccccattt ccaaactaaa tatcgcaaca gaacgatctg
     2941 tttttgttcc tacattttga gcaatattca atgcaaacgc tgttttacct accgctggac
     3001 gtgcagcaag aatgattagt tcttcgggct ggaaacctgc tgtcatttta tccagacctt
     3061 gatagccagt tggtagacct gtgatttctt cattgttttg agctagtcga tcgatatttt
     3121 caatcgtgga gttcagtact tcagcaatag ataaaaagcc gcttcgattt cttttttccg
     3181 aaacctccaa gatactgcgt tcggcatcat ctaaaattgt ttgaacttct tctccttgct
     3241 cgaatccttt cgtaacgatt cctgttgctg tctggatcaa tgtacgcagc aatgattttt
     3301 cttcaacgat tttcgcatag tagacgatat ttgctgctgt cggaactgct aatgccaagt
     3361 ctgagagata acttaaacca ccggcatctt ctaacaaatt ggcttgttcc agccggtctt
     3421 tgacagtaac cacgtcaatc gcttcattac gatcatttaa ctcgatcatt gtctcaaaaa
     3481 taatttgatg gccacgacga taaaagtctt ttggctctat gtactccatg gcatcaatga
     3541 tcgtttctgc gtcaagaaaa acagagccta aaaccgcttg ttcagcttca atattttgcg
     3601 gtgggattcg atcctgccac acttcattca tacgtatcgc tccttgtctt caattgcttc
     3661 attttacaag aaaatgagag aaaagtcatc attcgtaccg aaagtgtaat aaaaaaatag
     3721 ttctctttta ttatactcgt tttctttatg ctattttgct gttcccaaaa aagaaaccga
     3781 aatcaatctg aaatcgattt gcagcttttg ctttcattgc tgcagctgtt ttctcagata
     3841 aatttcggtt tgtctttttt ccttctggtt atacttacca gctgaacatc gctatcaaga
     3901 tttgttattc ttgtacgaca tgaactttga tcgtagccgt cacctcatga tgaagcttaa
     3961 caggtacgtt tgtgtagcct agtgaacgga tcggattttc taattcgatt tttcgtttat
     4021 ctaatttgat tttgtattgt ttgtttaacc cttcagcaat ttgttttgaa gggatcgaac
     4081 caaacaaacg gctgtcttcc cctgctttcg ccttcaattc gactacagta tcttcttttt
     4141 ccaagaattc ttttaacttt ttagcttctt ccaagatttc agcttcgtgt ttttcttgtg
     4201 ctttcttttg tcctgctaac tgactgatac tgcttttcgt tgcttctttt gccaagttat
     4261 ttttgattag ataattttgt gcataacctg ctggaacttc tttgatgtcc ccttttttac
     4321 cttttccttt tacatcttgt aaaaaaatta ctttcatttg gattactcct tatcatatat
     4381 ttgattcata ttttctttga ttgcagcaaa tagcttctgt tttgtttcct caactgtcgt
     4441 attttccagt tgagtagctg cgtttgtgaa gtgtcctccg ccacctaagg attccatgat
     4501 gatttggacg ttgatcgtgc cggtacttct ggcactgatc ccaatcaagc cgtctgtccg
     4561 ctttgtaatg acgaaagctg cattgatatt gatcatcgaa agcaatgtat ctgctgtttt
     4621 tgctgctata acactattat actctttttc gtctgtccct gccgccacaa caatatcaga
     4681 agtcacatag tcgcttccag caatcaaatt gctgatctcc aagtatgtcg ctaaatccga
     4741 gctcaataaa tattgtacta aggatgcgtc tgctccacaa gaccttaaat agctggcaac
     4801 gtcaaaagtt cgtgcagttg tacggacatt gaaactattt gtatctacaa ccatccctgc
     4861 aagcaataat gtggcctcaa aacgatctaa gcgatttgtt ttgctgcttt ggtattggat
     4921 cagctcagaa actagctctg aagctgaaga agctgaagat tcaatataag tcagcagagg
     4981 tttacttgga aactcttccc cacgacgatg gtgatcaatg atcacaatct tatcaaattg
     5041 ctcatatagg ccctgagaga tcgatagaga aggtttatgg taatccacca tcacgaggag
     5101 gtcctcttct tttttcagct ctatggcacg ttctggagag attagttgca tttctaattc
     5161 aggatgctta tggatttctt caagacaccg ttctacatct ggaatgatct cgttctcatt
     5221 gataacgatg taagcttttt tctggttgaa gcgtgccaga caactgacac caaaagcaga
     5281 gcctaaagca tccatatccg gaaacttgtg ccccatgata aacacatcgc ctgtttcatc
     5341 aaaaatccga cgcaaagcgg tactcatcgc tcgagaacga acacgcgtgc gtttgacatt
     5401 cttagcagat ttccccccat aataaacagg ttttgctcct tcttttacct ctttcacgac
     5461 aacttggtcg cctccacgga ctaaagcaac atctaaatta ttctgagctt ctccaccgat
     5521 ttcatcgagg gttcttgagc cataagcaat ccccatacta agggtgatag gcgagctctg
     5581 tttttcagct tctgctcgaa agcgatccag caaatcaaat tttttatcca tcatcttttt
     5641 cagatcgatc atctgtgcta cgaagaaaaa ccgttctgcg ttgattcttt tgaaaaagac
     5701 atggtattca ttcatccagt cagaaactag tgtagtgacg aaagagttca aataagaaat
     5761 ctctttgtca tctaatttat caatggcatc tgcatagtta tcaatagata aaataccaat
     5821 caccggctga ctgttcgtct gatggagttt taacaactgg ttttgggtaa tatcattaaa
     5881 ataaacaaca ccctttactg tatccatatc aaactgaaat gttttatctg ctagatagac
     5941 ttggtttttc tttttttctt ttgcagtaaa aaaagtatct agcaattctt cctctgttaa
     6001 tccactttga tctttgattt tcaatgctaa tggattgagc cattcgattt tttggcttga
     6061 tggctgataa gcaatcactc ctacaggcat gacttctgag acataagcta gattggtttc
     6121 agctacagtc gaagcctgct ggattttttc acgtttattt aaaataagcc aacgtttcat
     6181 tttcaaccaa tagcgaacac cccataaatt catcaataga atgaccgaag cgctaacaaa
     6241 acgaaatggt aaaattaaga ctaccaatac ttcgattaat ataaacagga taaggaagag
     6301 aggtttcggg gataaatgat tgtttttatt ttccataatt ttcctcccat atatgactaa
     6361 tttgatttta ccataacttt cgtcattcga gcgactaaac gaatagaggt tatttttaca
     6421 gaaaaaaagg aattgaatag ctgatcaaaa ggatcagtca ttcaattcct aggatttatt
     6481 agtcttcgct aacgaaaggt agcaagccca tgatacgagc gcgtttgatt gcaacagtaa
     6541 ttttacgttg gtttttagcg cctgtaccag ttacacgacg tggcaagatt ttaccgcgtt
     6601 ctgaaataaa tctttttagt aattcgatat ctttataatc gatatattca atatggttag
     6661 cagcaatgta gtctacttta cgacgtttac gtccgcctct tctttgttgt gccattctat
     6721 ttccctccta tcaaattcgt ttagaatggt aaatcatcgt ctgaaatgtc gatcgttgaa
     6781 cttgagccga atgggtcaga agtatcacgg tcgaaatcag gcataccgtt ggatgattga
     6841 gatgtttgat tagagttatt gtttccgcca aatccgccag ctgattgacc agaactttgg
     6901 aatccgccgc tttgttggcg attttcagat gcagaacgac tttccagcaa ttggaagttg
     6961 tcagctacta cttctgtcac gtatacacgt tgtccttgct gattatcata agaacgagtt
     7021 tggatacgtc cagtaacacc taaaagtgtt cctttacgag catagtttgc taaagtttct
     7081 gctgatttgc gccaaatcac acagttgata aaatcagcct cgcgattgcc gctttggttt
     7141 gtaaaattac gattcacagc caaagtaaat gttgcgactg ccgtaccact tgctgtgtaa
     7201 cgcaaatctg gatctttcgt cagacgtcca actaatacaa cattattaat caaaatagtc
     7261 atctcctctc atcgttttct attgtttcac atgaaacaac aattaagctt cttctttaac
     7321 aatcatgtga cgaatgatat cgtcgttgat tttagcaaga cggtcaaatt cattgacagc
     7381 atttgctgat gatggagaag atactttaac gatgtgatag ataccttcac ggtaaccgtt
     7441 catttcataa gcgaaacggc gtttttccca atcttttgat tcgataactt ctgctccgtt
     7501 atctttcaag attgcatcga aacgttcgat taaagcagtt tttgcttctt catcaatgtt
     7561 tggacgaata atgtacatga tttcatattt cgtgttttcc attgatattc acctccctgt
     7621 ggactttcgg ctcttaggtt cctaagagca aggagagtta tctaattaat tagactactc
     7681 acaagctcta attatacact ctattttggt caaaagcaat acagaatgaa cagtttcgaa
     7741 tattttaaga gaaattcaac taatcaaaaa agccagcagg aataaccaga tttt
//
LOCUS       DACEDM010000098         7731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEDM010000098 DACEDM010000000
VERSION     DACEDM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.100"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(10..1158)
                     /gene="nagA"
                     /locus_tag="IWO50_002590"
     CDS             complement(10..1158)
                     /gene="nagA"
                     /locus_tag="IWO50_002590"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0359664.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1234..2070)
                     /locus_tag="IWO50_002591"
     CDS             complement(1234..2070)
                     /locus_tag="IWO50_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0359665.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2254..3471)
                     /locus_tag="IWO50_002592"
     CDS             complement(2254..3471)
                     /locus_tag="IWO50_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0359666.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3854..3937
                     /locus_tag="IWO50_002593"
     tRNA            3854..3937
                     /locus_tag="IWO50_002593"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3888..3890,aa:Leu,seq:gag)
     gene            complement(4177..5547)
                     /locus_tag="IWO50_002594"
     CDS             complement(4177..5547)
                     /locus_tag="IWO50_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359667.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6261..6551)
                     /locus_tag="IWO50_002595"
     CDS             complement(6261..6551)
                     /locus_tag="IWO50_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359668.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6759..7451)
                     /locus_tag="IWO50_002596"
     CDS             complement(6759..7451)
                     /locus_tag="IWO50_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0359669.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         2357 a         1531 c         1183 g         2660 t
ORIGIN      
        1 taaattatat tatgcgtgga aacgttccac gccatctaga taagttgctt ccaatgtcat
       61 atcagggttc aatacgataa agtctgcatc gcgtccttga gcgatttttc cacatgtatc
      121 atcgatttta cagctgattg ctggaatcag actagccatc atgactgctt gttctggtgt
      181 tgctaatccc cagtctacaa catttttaat tgcttctttc aatttcaata tacttccagc
      241 taagttgcca gattctagac gtgctgttcc ttcttttact actactggaa attcgcctaa
      301 attgtaattt ccatcaggca tcccaccggc catcatacag tctgtaatta atgcgacatg
      361 atcatgtcct gctttttcca ttaggattcc cgcagcattt ggatgtacat gatggccatc
      421 acaaatcaat tcagaaaaga catgttctaa agataataac gcacccacca tacctggttc
      481 gcgatgattc aatccacgca taccattgaa agcatggaca aatacacttg cgcctgcttc
      541 aacagctttt tgcgcttctt ctagtgtagc attactgtgt cctagagaaa caactactcc
      601 ttcttcagtc acttgtttga caaactcttc tacaccttga cgttccggtg ctaaagcaat
      661 ttttttgatt aaaccacctg aagcttcttg ccattcatgg aaagtatcca aatcaggatc
      721 cccgaaataa cttgggtttt gtgccccttt gtgttcttct gtgaagaaag gaccttcaaa
      781 atagattcct ttgattttag ctcctggtgc ttcctgatac attttgccga ttgtctcagc
      841 tacatctttt aaacgttctt tgcttgacgt caatgttgtt ggtaaaaatg aagtcactcc
      901 acaagataac agaccttcag acatcacttt gatgccttct gcatcattat ccattacatc
      961 atgattcata tagccgtgaa tatgtgtatc taccaaacca ggagcaatcc atttgccgct
     1021 ttggtcaatg atgttgactt ctccctcagg tagttctttg gtgtattcac caaaaagtcc
     1081 atcggtaatt tcaagatagc ctgcatgttt gacacctgac gctaaaaaga atttgtcagc
     1141 atagataaaa gttctcattc tattctctcc ctttgttttt ctacctctaa agtactctta
     1201 tttagagaag aagtcaagaa tggtctacac catttacaaa ttttttgaaa tttgtttcac
     1261 agcttgtgct aagccttcat agaatgctgc tctttctggt tcttctttac gcttgtatat
     1321 taagctttta agcacaaata aatcattgag gtaatagtag ctattcttct cttgagccca
     1381 atagattcct tgattcacct gttctaacgc ttcgtcgatt tcattttgat ggaataagaa
     1441 atttccataa ttataaaaaa tttgcgaaaa ttcacttttt gatcgttcat cagctgattg
     1501 atgcagcagt tggatgcttc gactcaaata gtagcgtgct tcagccgttt ttcctgccac
     1561 accatatatt tttccgatac aactaatcag ttgtatttct gtgtcagaaa catggattct
     1621 ttctttttgg ctagagtagc ttaatgcttc tttcaaataa taaagggact gagagatatt
     1681 ttgacgcaac gtgaattcgc aaacaccgag ataataataa tagcgttgaa agtcagttgc
     1741 ttgatacaga ttttcagaaa tatcagaaga ttttagtact tgttctaact tccgataatc
     1801 acgtttttgg aaacaactat ccaacagttc aaataattta tcgtttgtcc gagtagtttt
     1861 tcgagagaga ttcatcacat gatcaatcgt tacccctagc cgttgacaaa tctgcatcat
     1921 caccaataca ttcggcagtt cctcattatt ttcgatccga cttaatacac tttgtgaaca
     1981 aatatcatgc gaaagcattt tttgcgtcat tttttgttct tttcgaatct ctttaatgat
     2041 cgttccaaag gactcgatta acgtttccat aagtatcctc cccaaaatat gcgtattcgc
     2101 attctattcg tattttactt taggaagtcg caaacttcaa gaaaagactt gcaactaaat
     2161 aatatacttt agttatttaa gaaaactcca ctagaaagag agcctcagtg tgtttgtctg
     2221 tggccctcct actgaaaaat ttggttttta aaatcatttt tggcgatagg ttgctaggaa
     2281 ttctctcatt ttcttagctg tttgtttcaa atcatccatt ttaggaagat aaacgattcg
     2341 aaaatgatct ggctggttcc aattaaatcc accaccatgt acaagtagaa tatgatgttc
     2401 atgtaaaaag tccaaaacga atttttcatc atccaaaata ttgaatcgct ttgtatcaat
     2461 ttttgggaat atataaaaag cagcttttgg ttttacggct gaaagaccag gaatatcatt
     2521 gattgcatta tagatatatt ctctttgttc atagatcctt ccgcctggca ggagcagctc
     2581 atcgacactt tgataaccgc ctagcgcggt ttggataatt tgctgagaca atacattgga
     2641 acacaaacgc atggaggaca acatattcag cccttctata tagcctttga cgtgtgactt
     2701 atctccactt aggaccatcc agcctactcg aaatcctgct acacgatgag atttggatag
     2761 accgttcagc gtaacaacga acaaatcagg cgctaatgta gcaatcggaa tatgttctag
     2821 accatccatc actaagcgat catagatttc gtcggaataa ataatcaggt tattttgtcg
     2881 agctacttca acgatttctt ctaataattc ttttggataa agtgccccag taggattatt
     2941 aggattaatg ataacaatag cttttgtccg actcgttatt tttgatttga tatcatcaat
     3001 atcaggatac cattctgcct cttcatcgca aatataatga acaggtgtgc ctccagctaa
     3061 tgcaatagaa gccgtccaca acggatagtc cggcattgga accagtactt catctccatt
     3121 gtccagcaac ccctgcatac acatcgtgat caattcgctc actccgttgc cggtgtagat
     3181 atcattgatc gtcacattcg gaaaatgttt cagttggcaa tattgctcaa ttgcttttct
     3241 agcagaaaag atccccttgg aatctgagta gccttctgag tcacgggcat tcatgatcaa
     3301 atcacgaatg acttcgtttg gtgcttcaaa tccaaaaggt gcgggatttc ccgtgttcaa
     3361 ttttaaaata ctaatgccct cttcttgcat ccgctccgct tcttctagaa cgggaccgcg
     3421 aacatcgtaa ctgacacctt ctaatttgtt ggatttttca aattctctca tcacttagcc
     3481 aatccctcat ttcaaaaagt tagaactttc caaatttttt gttatttact atttcaatat
     3541 catactccat cctagaaaat tttgccactt atttaggaaa aacgtaagta ttaaaaaaac
     3601 acaaacaagc gttgagaact taattgtcat gatagtcatt ccatagattc gctgcccaat
     3661 ccattgcaat tccacaaatg attcctagca taaaaccaag caaaaaacgt ccgaatgcaa
     3721 gtccaatcaa tacacctaag atcattccca gtcctgaaaa ttctccgtca tatttctcca
     3781 tcttaataag tcctcccatt tttgagcttg acaactatgt aaaacctcgc tatcattagt
     3841 gtgttattat tacgcagatg tgttggaatt ggcagacaag catgattgag ggtcatgtga
     3901 gcgttagctc gtgtgggttc gagtcccatc atctgcacta caactcgtga agttaatttt
     3961 gcttcacgag tttttttgtt tgaacgtttc tttatgccat tatatacata tcactgtatc
     4021 ctttacaaaa agaacgtaca atcatgtaaa cagcctgagg tgagtatatg aaggtcggaa
     4081 acaagctact cgtagaaact tgcaagtgtt aaaacaatta atcgaaaaac gagtaaaatc
     4141 cctcacctaa tactaaagcg agggattttt tgtatattat ctgtttatca ccgttattat
     4201 aaaggtacaa ataccaagca cggttaatcc aaattgcata aattcatttt tttgtacact
     4261 catgcttttc attagctctt taactgccat tagtactaag atggcgataa caacataaag
     4321 tatatagtac gtccgcattc cggaagccca tactgtggga gaaaatccca taattaacct
     4381 tgaacaaaat cccattatca tcaaaataac taaaaaaata gaagttttct tattgtcaaa
     4441 acttaaataa attccaacaa ttaaacatag taataatgcc aagactacta aagtagctat
     4501 ccaagtacct ggataaaaaa gtgacagttt cgttcctaac tctgtaaaga gattattcag
     4561 gttactacta ctccaaatca ttgccctttt atttccattg acgtaagtaa agctttgacc
     4621 cattgtatta ccaaaataaa taatcaggtt taagaaaaac ggtaatgcag tcaatatacg
     4681 cacataatag ttttgacatt tcctatatgt tagaaggaaa attaagaaaa atagcaataa
     4741 aaataagata ttcatgtcta aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa
     4801 cttattaaga aagttgaaat ttttatattc aggaaaccat ttattaattt catgtgcagc
     4861 acgtaatgca ttccctggag ttgtcaaaga gaaaattaat tctgctaagc ttattattga
     4921 aaatactgat aacttataat tatatcgtcc attgacaatc aaatataaac taactatact
     4981 tgttagaaca aaaaaacagg cgttgacttg ttcttgatta gttaaaaata tcaataaagg
     5041 aatacttacc caatatattt ttctatttat ttcttctcct cttaagagtt gaaaaaacgg
     5101 ataaaaagtt agtaaactaa atgctactgg ccactgatag ttcaaggtgg ttgctctcca
     5161 accggtttcc cccattactg tcaatggtat taaacagtat atagagcaaa ccaataataa
     5221 atttttaaca tttattttct caaaaacata cttgcaaagt aatactacaa ttcctaacat
     5281 aatcgtagaa tttaataatt tccatagcat aaagtgttta gaaaagaaca ttaagaatag
     5341 ttctataata attcttgaac tccatgtctt ataatgttct atcgaagcac ccaaatagcc
     5401 aagttcatct aatctacttg cataaactac gtcatccgct ctcatagtcg gcatatacat
     5461 attaaagagt aaaaagatac cccaaatccc tattagtaac aagatcagtt ctttgttatt
     5521 cgttattttt ctattatgtt tgttcatttt tatactcctt cttatatttc ctaatagagt
     5581 ataacatagc tttaagcatt acttaaagat aacttaaaag ttatctttta aatacttaat
     5641 tgttaaatac cttatttgat gcaaaagaaa tctacgatag tcaacaaaaa aattttcatt
     5701 atatccagaa aaatttttct tagttaagga cctatggatt gcagtaatgc agaattcgtc
     5761 gaataaactg ccaaagacat acaatcatat tgcttttaaa attgacgaac aagatataga
     5821 ttcgtttgtc tcaaaaatcc aaatgttagg tttaactgtc gaaccaggac gttctcgtgt
     5881 gaaaggcgag gcttcttcaa tttactttta cgactatgat aatcacctct tcgagttaca
     5941 taccggtact ctccaggaac gtttaaaatc ctacaattcc actacctgag actagtaatg
     6001 actagtctct ttttatggcc tctgatcccc ccattgagcg aatactaata gggcttcgaa
     6061 ttgatctgtt caatctttaa gcaatatccc tcaaatgagt ttggaaagtt tagtgattca
     6121 tcacaaatac ttaaatacat gtagttatat atagattgag gttcttgtga gcgtatgctc
     6181 gtgtgggttc aaatcccatc gtctgcatcc atctttccaa aacaagcggc taggacattt
     6241 ttgtctcagc cgcttgtttt ctagtttatt ctttcatctt gtcggacctt ttttcttggg
     6301 tatagacttt tctcatattc taccacaatt ttgggtttcc ctaacacatg ataggatctc
     6361 acaacaaatt gctttctatt attataataa cctgtaacga ctaacttcat cccttcgtct
     6421 acttcagcta ggaagttcaa actatgggaa gcaatcaaac aattgacccc atttaaactg
     6481 aaacggatca acggtctggc actaacttgc aaagtacgga tcttatctac tgttccatat
     6541 tttttctcca taaccttcac ctcaatacca ttatacgaac atacattccc tttgtaaaga
     6601 agaaactctg ttttatcttt ttgacttttt gtctattacc tttcttaaaa tcatattggc
     6661 acttcctgcg aagatgaagg acatgtacta aaaaaaagag gcagcagtat agattactgt
     6721 tgcttcttta ctaatttagg tttacttgtt aagaaagatt atttacgcaa atgttcttca
     6781 acaatttgct gagatttatc agctgtatct tccacttcat cttttgcttt ttctaacttt
     6841 tgaatatatt gtttcaagct tgagttttca gcagttgctt cgtctaattt cttttgtgta
     6901 tctgttaact gagtttctac atttgttttt tcttgtgtca gtgttgcgat ttgttgattc
     6961 aaaccattta cagtattttg taattctgaa agttttgctt gtgcttcttg tagtcttgct
     7021 gtccaatctg cattcagatg attgatttca tcattttttt ggttgatctc atctagtttg
     7081 acattgattt cattttgttt gctttgtagt tgtgcatcct tgctgactaa ttgactttct
     7141 aagttttgtt tttctgctgc cgattgattt ttttggtttt ctaattcatt tacttttgtt
     7201 tggtattctt ggacatctgc ctttgaatct tgcaatgtct gttctttttg tgctagtcga
     7261 gcggtaagat ctgtaagcgc ttgctcttta tctgtcaatg tatgatcaag ctgtgtcaga
     7321 ttctcattga ttgtatcaat atctgtctct ccaccccaca aaataatttc atttgcgtac
     7381 cgcgtgcctg tgtagccaat tagacataga aatgcaacga caatcccacc agtaataaat
     7441 ttctttttca tttgcatagc tccttaatcc aatactttta catgctaaaa gtaagtgatc
     7501 tcaaacacaa tgtcaagcgt ttttaaatta catgtgtaat tttaatataa atactatcaa
     7561 atactcattt aatcctcctt ttttatctat ttaaaaaatc ttttttatag agaaaaatgc
     7621 aggttaatac tttcttaaat aaatatttat ggtgaacaaa aacaaaaaag caagttttat
     7681 taaacgtaac ccttttcatt tcatagatta ctgtatttat tacaaaaaac g
//
LOCUS       DACEDM010000099         7625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEDM010000099 DACEDM010000000
VERSION     DACEDM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.103"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWO50_002597"
     CDS             complement(14..706)
                     /locus_tag="IWO50_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0359670.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWO50_002598"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWO50_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWO50_002599"
     CDS             complement(1143..1712)
                     /locus_tag="IWO50_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0359671.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWO50_002600"
     CDS             complement(1786..2109)
                     /locus_tag="IWO50_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359672.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWO50_002601"
     CDS             complement(2263..3075)
                     /locus_tag="IWO50_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359673.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWO50_002602"
     CDS             3429..4277
                     /locus_tag="IWO50_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0359674.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IWO50_002603"
     CDS             4422..5363
                     /locus_tag="IWO50_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0359675.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWO50_002604"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWO50_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2470 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacct
//
LOCUS       DACEDM010000100         7622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEDM010000100 DACEDM010000000
VERSION     DACEDM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.104"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..629)
                     /locus_tag="IWO50_002605"
     CDS             complement(105..629)
                     /locus_tag="IWO50_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0359676.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWO50_002606"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWO50_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0359677.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWO50_002607"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWO50_002607"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0359678.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IWO50_002608"
     CDS             complement(2351..3031)
                     /locus_tag="IWO50_002608"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0359679.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IWO50_002609"
     CDS             3157..4026
                     /locus_tag="IWO50_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0359680.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IWO50_002610"
     CDS             4052..4567
                     /locus_tag="IWO50_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359681.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IWO50_002611"
     CDS             complement(4614..5177)
                     /locus_tag="IWO50_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0359682.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IWO50_002612"
     CDS             complement(5232..5411)
                     /locus_tag="IWO50_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359683.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IWO50_002613"
     CDS             complement(5488..5997)
                     /locus_tag="IWO50_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359684.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IWO50_002614"
     CDS             complement(6093..6473)
                     /locus_tag="IWO50_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0359685.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IWO50_002615"
     CDS             6803..7552
                     /locus_tag="IWO50_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0359686.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2168 a         1580 c         1354 g         2520 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aa
//
LOCUS       DACEDM010000101         7596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEDM010000101 DACEDM010000000
VERSION     DACEDM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.105"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(135..425)
                     /locus_tag="IWO50_002616"
     CDS             complement(135..425)
                     /locus_tag="IWO50_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359687.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IWO50_002617"
     CDS             complement(543..803)
                     /locus_tag="IWO50_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359688.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IWO50_002618"
     CDS             complement(940..1353)
                     /locus_tag="IWO50_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0359689.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IWO50_002619"
     CDS             complement(1809..2294)
                     /locus_tag="IWO50_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359690.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2678)
                     /locus_tag="IWO50_002620"
     CDS             complement(2433..2678)
                     /locus_tag="IWO50_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0359691.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="IWO50_002621"
     CDS             2871..3671
                     /locus_tag="IWO50_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0359692.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5558
                     /locus_tag="IWO50_002622"
     CDS             3690..5558
                     /locus_tag="IWO50_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0359693.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5551..6042
                     /locus_tag="IWO50_002623"
     CDS             5551..6042
                     /locus_tag="IWO50_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0359694.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6030..6584
                     /gene="srlA"
                     /locus_tag="IWO50_002624"
     CDS             6030..6584
                     /gene="srlA"
                     /locus_tag="IWO50_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0359695.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6602..>7596
                     /locus_tag="IWO50_002625"
     CDS             6602..>7596
                     /locus_tag="IWO50_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0359696.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     EKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1213 c         1444 g         2233 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5101 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5161 aagaatattt aaacaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat
     5221 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5281 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5341 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     5401 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atgcgatatc ctatttgaac
     5461 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5521 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5581 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5641 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     5701 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5761 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5821 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5881 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5941 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     6001 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6061 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6121 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6181 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6241 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6301 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6361 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6421 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6481 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6541 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6601 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6661 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6721 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6781 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6841 aatttatcct aaaaaaggga taccaaccat caatatcgtt cccacaggaa aaagtggccc
     6901 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6961 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     7021 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7081 agaaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7141 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7201 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7261 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7321 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7381 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7441 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7501 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7561 cattgcttgg gtagcaagtt ttggattata tagctg
//
LOCUS       DACEDM010000102         7596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEDM010000102 DACEDM010000000
VERSION     DACEDM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.106"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            355..1839
                     /gene="lsa"
                     /locus_tag="IWO50_002626"
     CDS             355..1839
                     /gene="lsa"
                     /locus_tag="IWO50_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0359697.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTEFMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LLKEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAAKAMKRAKNIESR
                     HQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDKEVCSNLNFRVNI
                     GDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDTSYLKGNLSEFA
                     YNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCESAHLYIWDEPL
                     NYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            1893..2696
                     /gene="lnu(B)"
                     /locus_tag="IWO50_002627"
     CDS             1893..2696
                     /gene="lnu(B)"
                     /locus_tag="IWO50_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAQ0359698.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            3384..4205
                     /locus_tag="IWO50_002628"
                     /pseudo
     CDS             3384..4205
                     /locus_tag="IWO50_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4348..4572
                     /locus_tag="IWO50_002629"
     CDS             4348..4572
                     /locus_tag="IWO50_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0359699.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            4587..5456
                     /locus_tag="IWO50_002630"
     CDS             4587..5456
                     /locus_tag="IWO50_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0359700.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5437..6171
                     /locus_tag="IWO50_002631"
     CDS             5437..6171
                     /locus_tag="IWO50_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0359701.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6204..7112
                     /locus_tag="IWO50_002632"
     CDS             6204..7112
                     /locus_tag="IWO50_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0359702.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7109..7357
                     /locus_tag="IWO50_002633"
     CDS             7109..7357
                     /locus_tag="IWO50_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAQ0359703.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(7360..>7596)
                     /locus_tag="IWO50_002634"
     CDS             complement(7360..>7596)
                     /locus_tag="IWO50_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0359704.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         2635 a         1094 c         1561 g         2306 t
ORIGIN      
        1 ttttcttgct aattatatga tgcataataa gatatttaaa gaaacggcta tagaaccgtt
       61 tgttttgtta tgcaaattgc aagaaagtta agtattctac actcaataat acattacagt
      121 aaccttatgt tatgtataac aaaaggtaat aatagtattt gtttttgagc cgtaagaatt
      181 tactttcttg cggcttttaa tatttgtaaa agaaaggaag ataaacgtga tagacaatga
      241 tactatgtca ttcatgacaa aatccttgca gagttggata acattataga gtaattaatg
      301 ccgaaaaccg actttgctta taaaattttt ataatgtaaa ggaggaagaa atatatgtcc
      361 ttaataaatg tttcaaatct aactttttca tatgaaggaa gttatgacaa tatttttgaa
      421 aatgtaagtt ttcagataga tacagattgg aaactcggtt ttattggaag aaacggacgc
      481 ggtaaaacta ctttcttaaa tttactgctt ggcaaatatg cgtattccgg caatataagt
      541 tctacagtta agtttgagta ttttccttat gatgtggaag ataagagtct atatacaatt
      601 gaagtaatga agagtatttg tacggaattt atggattggg agatttttcg tgaaatatca
      661 ttgcttgatg ttcaagaaga tgctttatat cgtccgttta atacattgtc aaatggtgag
      721 caaacgaagg tccttcttgc agctttattc cttacagcga gttgtttcct gcttattgat
      781 gaacctacaa accatcttga catcgatgca cgtaatgtag tgcaaaacta tttgaaacgc
      841 aagaaggggt ttattttggt atctcatgat agaagcttac ttgatcaatg tgttgaccat
      901 atactatcta tcaataaaac gaatatcgaa atccaaaagg gaaattttac ttcttggtgg
      961 gagaacaaaa cgttacaaga taattttgaa ctggcagaaa acaagaaact ccttaaagaa
     1021 ataggaaggt tgtcttatgc agcaaaacgt agttcaaact ggtcaaataa agtagaaaaa
     1081 agtaaatatg gaacaacaaa ttctggttca aaactggata agggttatgt tggacataag
     1141 gctgcaaaag cgatgaaacg tgccaaaaat attgagtcaa gacatcagga agccgtttta
     1201 caaaaatcag aactgctcca caacattgaa caatatgatg acttaaaaat ttcaccactt
     1261 gaatttcaca aagagtgctt aatagaagcg aatgatttat cattgtctta tggagataaa
     1321 gaagtatgca gtaatcttaa tttcagagtc aatattggtg atagagttgc cattatcgga
     1381 aaaaatggga gtggtaagtc tagtatccta aaattgatta atggagatga tattaaattt
     1441 accggaaatt ttatgctagc aagtggacta aaaatttctt atatttcgca agatacttca
     1501 tatttaaaag gtaatctatc tgaatttgcc tataataata agatcgatga aactctattt
     1561 aaaacgattc ttcgtaaact ggattttaat agagagcagt ttgataagaa catggtggat
     1621 tttagtgctg gtcagaaaaa gaaagtacta attgctaaaa gcctttgtga aagtgcacat
     1681 ttgtatatat gggatgagcc attgaactat attgatattt tttcacgtat ccaaattgaa
     1741 aaaatgattt tggaatattg tcctacacta ttgtttgtgg agcatgatga tgctttttgc
     1801 aataacattt gtacgaaaaa tattaattta ggtttgtaga gattttgagt tgccacaata
     1861 actaaaagat atttacatga aagggtgaag aaatgttaaa acaaaaagaa ttaattgcaa
     1921 acgttaagaa tcttactgag tcagatgaac gaattacagc ttgtatgatg tatggatcgt
     1981 ttaccaaagg agaaggtgac caatactctg atatagagtt ctatatattt ttgaaagata
     2041 gtataacctc gaactttgat tcatccaact ggttgtttga cgtagctccg tacttgatgc
     2101 tttataaaaa tgagtacgga acagaggtag ttatttttga taatcttata cgtggggaat
     2161 ttcatttcct ttctgaaaaa gatatgaaca taatcccctc gtttaaagat tcaggttata
     2221 ttcctgatac gaaggctatg cttatttacg atgaaacagg gcaattagaa aattatttat
     2281 cagagataag tggtgcaaga ccaaatagac ttactgaaga aaatgctaat tttttgttgt
     2341 gtaatttctc taatctatgg ttgatgggaa tcaacgttct aaaaagagga gaatatgctc
     2401 gttcattaga actcttatca caacttcaaa aaaatacact acaacttata cgtatggcag
     2461 aaaaaaatgc tgataattgg ctaaacatga gtaaaaacct tgaaaaagaa attagccttg
     2521 aaaattataa aaaatttgca aagaccactg ctcgattaga taaggtagaa ttatttgaag
     2581 cctataaaaa ttctttgcta ttagttatgg atttgcaaag tcaccttatt gaacaataca
     2641 acttaaaagt tacacatgac attttagaaa gattgttgaa ttacattagt gaataggaga
     2701 gtaacgttat tcacagtatt gggtactgaa aattaacagc atacctcagt ttttcaaaaa
     2761 acagcgaagc ctatggatat gttcccgtct accgatagtg caaaaagtgt cgtggatatg
     2821 tttccgagaa cggacataca caaatgacat tcattctgtc ctctcaatcc atgtggaagc
     2881 agtatgtctg ttaacaagaa aataattatt gttcgtcctg acgagttgtt agactcgaaa
     2941 aggataaaca tcatttttag aaccggtagc cgatttttgg ctagcggttt tttgggctct
     3001 ttggcaaata gtggtgattg aatctcgcgc tatcctatgg aaaaccgcct tgtgtgaggg
     3061 gctgcgagac cgcttcgatc ctagaattgc tgaagcacga atctagcgtc actcacattt
     3121 catcttttga cgtagctgag acaaggtgtt tttcttcgat ccgctagtgg ttaatctctt
     3181 tttggtgcaa atatggttta gaatttcttt gcatggctta agggagtgga gaatcggata
     3241 ttagtaggat ttacatccac agataaaaat aggcttgatt ttaacagcga aactaaaata
     3301 ccctacttaa atgaagtgat agattagatg ttgggttgtt aggagatgct taaaaatcct
     3361 atgcaatcaa tagtttttag tgtttgttga cttgatccct aaatcgcctg agtactaaac
     3421 gttgagtgtg tctgcttact gtcactagcc agtaagtgag actgtagtta atctctcatt
     3481 ttccccagta ttatgttgaa aatagccatg aagcgattat ttctaaagag ttatttttgc
     3541 aggctcaaga agaaatttat cggagaagta atatctacac aggagaaggc aagaacaaaa
     3601 gaatttatag tagcaagtac gctttaagtg ccatcacctt ctgtggatat tgtggcgata
     3661 tttataggag aacctattgg aatattcatg gcagaaaaga atttgtctgg cgatgcgtga
     3721 ctagaatcga gcagggtccc gaggtatgta agaaccgaac cgtaaaagaa gatgaactct
     3781 atggtgcggt aatgaccgca attaataagc tacttgctgg tggcaacaat atgataaaga
     3841 cactggaaga aaatattcat gctgtgattg gagaaaccac agaataccaa atttcagaga
     3901 ttaataccct actagatgaa aaacaaaaag aactaatcaa gctggccaat aaaggtcaag
     3961 attatgaata cctggtagat gagatcgatg agatgcgaga taagcgtcag actcttttag
     4021 tagaagatgc ctccctcagt ggcgagaacg agcgcatcaa tgagttgatt gaatttatcc
     4081 gcaagaacaa attccgcacc ttagaatatg atgataagct tgtaaggaag ataatccaga
     4141 acgttaaggt ctatgaagac cacttcgtca tagccttcaa accaggtatc gaaatggaaa
     4201 tatgaaaacc agaagtaaat agcccatgac tctgaagtag agttgtgggt tttcttttaa
     4261 ttgctgatta taataatatt ttaatgacta ttgcatttat caaccaaatg gtttatgtta
     4321 ttcttagcat aaacttggag gtgctgtatg actacggcag aaatgattaa agaactgtgt
     4381 gagcaaatga atataagtgt ttccgaactt gctagacgta ttggccagac tccacagaat
     4441 tttaataaaa aattacaacg agaaacagta accttggatg agttgaaggc cattgctgat
     4501 gtgctaggtg tcaagtttgt gcaggcattt attttaccag atggagatga aataaaaata
     4561 tctaacgaat aaaggaggat gtttatatgg taggtaatgt tattcaaata gtaacagaga
     4621 aattatcatc tttgcctttt atagaaggca ttgtattagg gggctcacgt gcaagaagca
     4681 cccatacaga aaattctgat atagatatcg gaatctatta caattcagac tcatttgacc
     4741 tgacagcgat taatcaaatt gctacagagt tggatgatga gaatagaaac aaccttgttg
     4801 tacctcccgg agcgtggggt gattgggtta atggcggtgg atggttagtt ataaacgggt
     4861 gtcatgttga cttgatatta cgtgatataa aacgggtgga acaaataatc aaagatacgg
     4921 agcaaggaat tgttactgcc aattatcaga ccgggcatcc ccatggttat ataagtgcta
     4981 tgtatcgtgg agaattagcg attagtaaaa tactatatgc taagaacgaa agcttatgcg
     5041 aattaaaaaa gcaggcagaa atttacccta ctgctctaaa gaaaagcttg atgaactttt
     5101 ttatatttga agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg gcagaggata
     5161 aatattatat tgcaggccat gtttttcgta ttatttcatg cttaaatcaa gtactatttg
     5221 catgtaataa tgcttattgc attaacgaaa agaaagctat aaaactgctt gaaacttttg
     5281 aatataaacc tgaaaaatat gccgaaagag ttaatcatat ttttgaagta ctcggtcttt
     5341 ctctttttga atgctacgac atgaccgaga aactttataa agaagtgaat gaaattgtat
     5401 cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat
     5461 aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg
     5521 ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat
     5581 ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta
     5641 gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca
     5701 cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat
     5761 gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag
     5821 atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt
     5881 actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg
     5941 gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca
     6001 aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata
     6061 aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat
     6121 gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga
     6181 aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt
     6241 ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata
     6301 ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag
     6361 aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa
     6421 agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat
     6481 ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc
     6541 taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca
     6601 tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt
     6661 gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa
     6721 ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat
     6781 catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta
     6841 ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt
     6901 cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg
     6961 gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat
     7021 ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg
     7081 ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa
     7141 agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga
     7201 aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca
     7261 agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga
     7321 taagataata tatcttttaa cccgtggtgc tagttaattt taatatccta aagaatatct
     7381 caaagttcct ccaaaacgtc gttgtgctcg acttaataga acactgtaag ccagtaatat
     7441 gctttcaaat ctactctcta gccctttaac agaacgtgag ttgaaatttt gacaacctaa
     7501 tttttctaag gaagaaaaaa cagtctcaac tgtttttctt tgttttccaa gtaagctgtg
     7561 gtctagtttt tctgatttat ccatgttttt acgtgg
//
LOCUS       DACEDM010000103         7586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEDM010000103 DACEDM010000000
VERSION     DACEDM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.107"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            127..579
                     /locus_tag="IWO50_002635"
     CDS             127..579
                     /locus_tag="IWO50_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359705.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IWO50_002636"
     CDS             593..1282
                     /locus_tag="IWO50_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359706.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IWO50_002637"
     CDS             1407..3758
                     /locus_tag="IWO50_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0359707.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IWO50_002638"
     CDS             3838..4233
                     /locus_tag="IWO50_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359708.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IWO50_002639"
     CDS             4558..5175
                     /locus_tag="IWO50_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0359709.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IWO50_002640"
     CDS             5216..5515
                     /locus_tag="IWO50_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359710.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IWO50_002641"
     CDS             5549..5716
                     /locus_tag="IWO50_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0359711.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6324)
                     /locus_tag="IWO50_002642"
     CDS             complement(5761..6324)
                     /locus_tag="IWO50_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0359712.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6352..7032)
                     /locus_tag="IWO50_002643"
     CDS             complement(6352..7032)
                     /locus_tag="IWO50_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HAQ0359713.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
     gene            7105..7383
                     /locus_tag="IWO50_002644"
                     /pseudo
     CDS             7105..7383
                     /locus_tag="IWO50_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
BASE COUNT         2581 a         1081 c         1489 g         2435 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gtttggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttcggt
     6301 tctgttgcga agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat cttaggctgt
     6361 tattcccatt aataccttga tttcagtaga caccgaaaag ccgaagagcg ttccatttct
     6421 tcggttcttt ttatatattc ctcgaatggt ctccatgccc ttaatcgtgg aagaggctgt
     6481 acggagactt tgataaaatt tatttcgtcg tttaataggt cgatggtctt gttctattaa
     6541 attgttaaga tacttcacag ttcggtgctc tgtcttagta tataaaccca cactctgtaa
     6601 ctttctaaag gcggagccaa gagaaggtgc tttatcggtc acaattgctt tcggctcacc
     6661 aaactgttta tggagtcgtt ttaagaaagc ataggctgct tgcgtatccc gtttctttcg
     6721 taaccagata tctaaggtta agccgtccgc atcaattgca cgataaagat aatgccaacg
     6781 tcccttaatt ttgatatagg tttcgtccat tttccatgaa tagaaggatt gtctattttt
     6841 cttcttccaa agataataga ggactttgct gtactcttgc acccaacgat aaatcgtagt
     6901 atgacaaaca tttattccac gatcatataa caattcctga acttcacgat agcttagatt
     6961 gtaacgcagg tagtaaccaa cagcgacaat aatgacgtct tttttgaatt gtttgccttt
     7021 aaaatgattc attactctgt cctctctgtc ttttttctca attttacact aaaatagatt
     7081 ttttggaaaa ctttgcaaca gaaccaatat gaaaaagtac aaatagttga tgttaataat
     7141 ggaaatagat ttgaaactta tacaatagct ggagagagag gaacagggat gatttgcttg
     7201 aatggagctg cagctagatg tgtatcaact ggagataaaa taattttaat ggcatattgt
     7261 gaattaaatg aaaatgaggt taaggatcat agacctaagg ttgtttttgt cgatgataat
     7321 aataaagttg aaagagtaac atcatatgaa aaacatggaa ggttatcaga tataattctt
     7381 taggttttgt aatgttattt atatatagct agactattat tgaaatattt attgattact
     7441 aattggatag atgtcaataa agtagacaca aaaagagaag atacagactg tagacaaagt
     7501 ccattttatg tgaatcggct acactagact agacagaaaa aataaggtgt gtagaataat
     7561 aaaaaacaca ctggaggatt tttcat
//
LOCUS       DACEDM010000104         7574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEDM010000104 DACEDM010000000
VERSION     DACEDM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.108"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            355..1173
                     /locus_tag="IWO50_002645"
     CDS             355..1173
                     /locus_tag="IWO50_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0359714.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            1192..1599
                     /locus_tag="IWO50_002646"
     CDS             1192..1599
                     /locus_tag="IWO50_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359715.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            1918..2949
                     /locus_tag="IWO50_002647"
     CDS             1918..2949
                     /locus_tag="IWO50_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0359716.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTETDQLQNQVLLDNFVEIMK
                     EDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKAEELEKTVVVYEELESYGI
                     DADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEKAIAAKLANEQRETFNELP
                     TVSTENWLE"
     gene            3807..4325
                     /locus_tag="IWO50_002648"
     CDS             3807..4325
                     /locus_tag="IWO50_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0359717.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            6401..7486
                     /gene="xerS"
                     /locus_tag="IWO50_002649"
     CDS             6401..7486
                     /gene="xerS"
                     /locus_tag="IWO50_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0359718.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         2870 a         1094 c         1217 g         2393 t
ORIGIN      
        1 gtatatgatc taagtcttgc ttctagtatg aatatgaccg atgtatacga aaagttatta
       61 atactagaaa atgaatatga tcaattcaat tcaaatcagc agcgaaaagt aaagaaacag
      121 atagactaga atagctattt tcgaaaagca atatttaagg tataagttat accttaaata
      181 ataaaagtat aagttatacc ataaatatat atggtataac ttatactttt attattaaaa
      241 accgctatat ccaaacgttt ttagatatta ttatttaagt tatactttta atgttgacca
      301 tttccgatat ataagttata ctttaattat caattaaaga ttgggaggat ttacatggat
      361 acttttcaag ggactacatt tacagttggc aattttaaag gaggagtagg taaaactaag
      421 atagtcagca tgcttgctta tgacaatgca atgataagaa atagaaaaac gcttattatt
      481 gatttagacc ctcaagcgaa cttaacacaa gtcatagcaa gaactttcgg tataaccgat
      541 atcaaaacca ctataacaaa tggtgtcgca aatggagaat tagcaagtag tattattaag
      601 gtaaatgaaa atcttgacct tctggcatgt gacactagtt ttcgatcatt ctccgctttt
      661 gctaataaac atgaaaatga attagaccaa gttactgtct tgaaaaaatt acttcttcca
      721 ataaagaaca attatcaaga aatatttatt gatgtaccac caaccattag cgaattttcc
      781 gataatgcta tggcagcttc agactattcg ataatttcat ttcaaacagc tgaagaatcc
      841 ttagagggag tcaacaaata tgtaaattat caaaaattca tggtcgatag atacgatctt
      901 gatttgcaaa ttatagatat tgtaccttgt atggtagaac caaatgatga ttttgatgaa
      961 gaaatcctag aagaagcaat tgaaaaatac ggaaccgtgg tatccaaaaa tctcattcat
     1021 tatcaaaaaa gattgagaag gtattctaaa attggtatac atttaagaaa atacaaaaat
     1081 ggaaattttg atcaatggga ttataaagca caccaagtat ttataaatat tttggcagaa
     1141 ctagatgcta gacaagagtt tttaaataaa taataaggag ttgcataagt aatgcctaaa
     1201 gattttgata ttcctaaaat gatggagaat ggtgaatttg agccattatt acccaataac
     1261 aaacgccaag atgtgaaaat aagcggtgaa tataacttta gcaatagtgc attgagtaac
     1321 acaagaaata ttacattgaa aaaaagaggc cgaccagttt caattcctga taaaagaaac
     1381 aaggtatcag ttcctaaaaa gatctctccg gcattaaata gcaaactctc aattttacaa
     1441 gactatatga cggagttaca aagtgaaaaa gggagaattt ctttcgaaaa aattgttaat
     1501 acattagcag actcatatat ccagcataga ctaggagtag caaaagaaga acacataaga
     1561 gaagaaatac aagaagagtt tgaaaaatta ccaaaataat ttttttcatg cggtattcaa
     1621 ttaaagtata agttatacta gataaatgaa tattaaagta taagttatac attaaatatt
     1681 taaggtataa cttaataaac aacgtgtaaa tttgcgatta aatcaacatt ataaaattat
     1741 tatttaaagt ataagttata cattatttgt atttaaagta taacttatac ttattttttt
     1801 aatatataac ttatacctta aatactgaaa gtgttttatt cacccttaat acatggtata
     1861 gttcacttgt tgaaaatcct gaatatatac aagtttctaa gaagaaggag aaatttaatg
     1921 tctacagaaa atcaatctca aaaaatgata attaaaaatt ggggatataa agcaacgaat
     1981 gtttatattg aaaaatatta taaatttcct agttttttaa ttcagaattc taaatactct
     2041 aaaatgaaag acagctcaaa aatagcctat atgcttttta aaaatgaatt tcgtagagcc
     2101 ttacatgaaa aatggatcga cgatgatggt ctattatatt tggaatttac acaagcagac
     2161 ctaatggaca aattaaattg ctaccaaggc aaagtaaaaa ccattttaaa cgagttagaa
     2221 acatataatt tgatttcagt cgtaaaagga gaatttaacc caaaaacgaa aaaaaatgaa
     2281 aaaaataaat actatttact tcaacctgaa ataagtgccg atgatctttt tattcaaaat
     2341 tctttcgatg acgatttaag cgaaactatt gataaatcaa cctcttctcg gaatgcggaa
     2401 atcgcacgtc gagaaaaaac agctgaaacc cttgataaca ctggaaatgc ggaaatcgca
     2461 caagattatt atattaataa atctttagac actaaaagac acttaaaaga cactgaaaca
     2521 gaccaattac aaaatcaagt tttgctagat aacttcgttg aaatcatgaa agaagacagc
     2581 ataaacacat ttgtaccaga aaaggtatta agcttgataa aaacattttc taacacttac
     2641 gaagaagccc aacaaaccgt caccacaata cacaacgcaa aacataaagc cgaggaactt
     2701 gagaagacgg tagttgtgta tgaagaactt gaatcttacg gaattgatgc ggataaagga
     2761 ttatatatga cgttgctgaa agcttatcaa aaacaaaaga ctgagaaagt aaataatttg
     2821 cagaatttga tttttgtgta tgtaaaaaac tggtttattg aaaaagctat tgcggcaaag
     2881 cttgcaaacg aacaaagaga aacatttaac gaattaccaa cagttagtac ggaaaattgg
     2941 ctagagtagt tgataaaaag cgaacactat aaaaatgatc aattttaaat tagaactttt
     3001 ttacaatttt aattttatta caaatgttac aaataaatag ttattattcc ggagttttta
     3061 gattaaaagt ttatgaaaac aatcacaaac gtaggtttta atgtcataat cgttagatat
     3121 tttagttatt tttcattcaa aaaaacaggg aactagtgtt tgcatatgta tttttttgag
     3181 ttataataat tcatgaagag aggtaattca cttatttacc caagctgaaa agcctgatat
     3241 tacttctttt caggcagtgc tgctaatatt caattacact tttttgtata aatttagttg
     3301 ttttaaattt aattgtctgc tatgcttgtg atgaattgat tttcgttctg cacttttgga
     3361 cgataaattt ggacaattga aaatacaaaa aaaaagaaag gccctgtgag actcggaagg
     3421 ctaccaacct tcgtcgacgt ccacaggtgg cattaagatc actcccttaa tgcgtttacc
     3481 tttcagtgtt acggtattac ccgtaacgga tttagtataa cagatattca aaagtgatac
     3541 cacttttttt ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa
     3601 gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattagg
     3661 agacgttggg atattttata aatcattcac atatgagtta gacagtcaat gcatatcaga
     3721 tatgcattgt agaacggatt aaaatatcgt ggatgaaatt atagatgaat taaaggagac
     3781 aattaggaat ttatggaggt aatataatga gaacatataa aaataaagaa gaattaaaaa
     3841 aagaaataaa taggacttat actaaatata ttttagaatt tgaaactatt cctgaagaat
     3901 tgaaggataa aagagttgag gaagtggata gaacacctgc tgaaaattta gcatatcaag
     3961 ttggttggac gaatttaatt ctaaaatggg aaaatgatga aagaaatgga ttatctgtta
     4021 aaacgccttc tgatcaattt aaatggaatc agttaggaga gttatataag tggtttacaa
     4081 atacctatgc acatctacca ttgaaagaat tagaggaaat tctcaatcgt aatattgatg
     4141 atattaacat gatgattgat tcaatgagtg atgaagatct attcactgca cacaaaagaa
     4201 aatgggcaga cgaggcaacc aaaacggctg tatgggaagt atataaattt attcatgtaa
     4261 atacagttgc tccatttggg acttttagaa caaagataag aaaatggaag agattagctt
     4321 tgtaaattcc cgtttactgc taagcaattg tggaaaggag acctcttgta tgttaacagt
     4381 aagaacatac gactacataa gttctttgct aactgccggt ttttattggg tgaatgcaag
     4441 tgcactcccc tagaatgccg tagagggtgc atcgttagat tgtatcaatt ttggttggcc
     4501 aacagataaa ccatgactca cttcgataaa atgagattat ggtgccatat ccgctacaaa
     4561 gggacttagg agactagcag gggaaagatt tttttatttt tgggatgttt ttcagaccgc
     4621 cgatagtacc gacaattccc tttaaatatg gaatctcaac aacccacatg aagggtaagg
     4681 caagtccggt aattatatct tctgactttg ggttaggaag aattgaaatt gttaagaacg
     4741 attcaacgct tatggtttat aatttggaga tagcctttgt taatatgcta aagccaacat
     4801 acgatacaga ttttgagcag tttattctaa ccatcaaagg gtatgtggcc tatcgcctat
     4861 cctattactg atactgaaga gatcttaaat aaattctata cgggatttga agatcctgaa
     4921 ggtgaaatcg tcatttatca gaataaataa atcaaatagt agaagttcaa agaatgaggg
     4981 gaacattagt ttttattgct tagtttaatc ccagtaccga tttctgcaag atgtaaaagg
     5041 tctttcattt cataagtttt cagaagcaag ttcatacgca ctaactcact ttcgattgag
     5101 tagcaatcgt caacaaactt tactatcagc tgttattttt attctttcgt gttatctaaa
     5161 taccatcaat ttataagttg taggtgtatt taataaaaga acaaataact ttagtatttt
     5221 tattaatcta acttacaaac tagtagtata ggggtaaatt tgcgattatt cataaatata
     5281 ccaagtaaaa gtatagggta tagtaaaaat atgataaaat aaatagtcgt tatcttaatc
     5341 caactatata gaaaatttac gttttctata tagttggatg tttacaagtt atatctggtt
     5401 taataagttt ttttctgtta gtcatggcat gttttattgt gctggttcat caatatattg
     5461 aaatgtacta aaatgaaatt ttcagttatg gtataatatt tatgctgtct gctaccccgg
     5521 cctattatgg caaggggagg aggttgtatt atgaattacc ttttcgatat gttttttgca
     5581 atcttagcag aagtatccgc gcattacatc tgtaaatggc ttgattgcaa aattattgcc
     5641 aaaaaagaca gtaagcctac ccgttaatct gaatagaacg taaaagaaaa ccactagccc
     5701 gccagctagt ggtttttggt gtatcatgaa ttaccttttc gttaaaacaa ttataccata
     5761 tttcctaaaa aatacaatta atagacgaat tatcattacc actttgatga aaaaagctga
     5821 cataaatttt tcttttattt ttataatgaa taaacattta tcaataagct tttgaaaggc
     5881 aggatcgtac tttgttgatg attgagatat tgctacatca atctcagcag ttaagatttt
     5941 tttgaattgt tgcttgaatt ttttctttat ctaaaaaaat tgtatctcca ggatgcaacc
     6001 cattttcttt aagaaattcc ttttaaattg ttaatactac tgagtgcccc acttctctga
     6061 attttcttta acttctccct cccctttttt atttgtatat acattcatat acgcttgtta
     6121 atatcaaatc aaccgtagtc tctcttatgt ctaattatat agaaagattg ttctttctat
     6181 ataatttaga caatttaaaa aattaagatt gcctccttct gtttcctttc ctattcatgt
     6241 ataccattta ttttaaatta ttaaatctat acctatttat ctgctttttt gaaataaatt
     6301 aataaatttc agagtatacc aattgacaaa acactctata attggtatag tccaattata
     6361 gagatactcc tctcccctac ttatcgaaag gatgaataac atgaaaaata cagaaatata
     6421 tcaaaaaata gatacaatca tacttcatat gcccgattat ataaaagatt ttgttcaaga
     6481 tcgtgaggat aaagatcaat cacctcgaac tactttagaa tatctaaaaa attataagct
     6541 cttttatgaa tggcttctga gtgagtctat cgttcccgat acttcaatca cctcgataag
     6601 acagttaact gcctatgatc taaacttgta caaatctcat ttgaaacgtc gcacaaagga
     6661 aaatgtaaaa aaagagacaa caaaaactaa gctcaaagat aataataact taggtctatc
     6721 tacttctact atcaatagaa atatcacagc attaaaggtt ctatttaaat atctttcgaa
     6781 aagttccaat aatcctttag gaaaaccata cttagaaaat aatcctatgg atcaagtagc
     6841 aactatcaca gataaaacta cgttagctgc ccgtgcaaac gcaatcgaga aaaaattatt
     6901 tctagacgaa gatacacaaa attatctaga ttatattgcc aatgaatata aaaaaacttt
     6961 gagcaaacga gctttgatct attatcaacg agatgtagaa agagacctgg cgatcaatgc
     7021 attgatttta ggatctggat taagactctc cgaggttgta aatattaatt tagaggacct
     7081 atctttagaa aagaataatg tggtagtaac tagaaaagga aataagagag acgcagtaaa
     7141 tatagcagca tttgcaatgg aatatttagc taattatcta gctattcgga aagaacgata
     7201 caaagtaaca gaaaacgaaa aagctttatt tctagcaatt tatcaaggag aagccaagcg
     7261 tatatccggt attgcaatcg aacggatggt agcaaaatat tcaaaaggat tcagagtcca
     7321 agtcagtcca cataaattac gtcatacttt ggctactaga ctctatcaac agacaaattc
     7381 attggtatta actgcccaac aattgggaca tacaagtaca aatacaacaa ctttatatac
     7441 tcatatagac aatgcagcta caattgatgc tttgaacgct ttgtaaatgt agtaaaaaat
     7501 agtataataa tgaagcctgt ctctaaaatt agataaaagc ccaactttaa aggcaagctt
     7561 cattatcatt ttta
//
LOCUS       DACEDM010000105         7499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEDM010000105 DACEDM010000000
VERSION     DACEDM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.109"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1087
                     /locus_tag="IWO50_002650"
     CDS             5..1087
                     /locus_tag="IWO50_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0359719.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IWO50_002651"
     CDS             complement(1305..2447)
                     /locus_tag="IWO50_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0359720.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYMLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTIEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IWO50_002652"
     CDS             complement(2452..2637)
                     /locus_tag="IWO50_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0359721.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IWO50_002653"
     CDS             complement(2991..3236)
                     /locus_tag="IWO50_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0359722.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="IWO50_002654"
     CDS             complement(3230..3631)
                     /locus_tag="IWO50_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0359723.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="IWO50_002655"
     CDS             complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="IWO50_002655"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0359724.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5648..7414)
                     /locus_tag="IWO50_002656"
     CDS             complement(5648..7414)
                     /locus_tag="IWO50_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0359725.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         2405 a         1439 c         1032 g         2623 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct gtgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caatggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacatata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcctt ctttaaacag aaaatctttc tataaaaaac aaaaaaaacg attaggctag
     3781 gctaatcgtt ttttgaacat tcttaaaaaa tccaaattat tttttataac tgaaattggg
     3841 tattgatgac catcgtttct gtagatagta agcaatcatt ttaggttttc tatctctagt
     3901 aaagattcct ttcttatttc cttgcactct taaaatccct tgacttgttg caaaatctgc
     3961 aaaattccac acttgttcac caacaaaatt ttcacatgta tctattactt cgtgatttgc
     4021 ttcgtaataa gccacttgat actcttctgt aaacattgtt ggtactgtat catgaaatcc
     4081 agctactgta tctgctccat attctgtaaa cattattggc gtatcaggac atctttccat
     4141 ccatccatct agctcttttt tcaacagata ttttgcatgt gccagatccc ctcccgaaac
     4201 ataccaacca taataacgat ttaaacataa aacatcgaca atatctccta ccttacaaac
     4261 atctggcgtt gattttagat aagtaacgat tgttacaggt cttttctgcg gatcaagttc
     4321 tttagttaat tcaactaaag gttcaaaata ttctttagca ccttcggaat ccgaatctgg
     4381 ttcatttgca atagaccaca ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc
     4441 cttgatcacc gttttatgag aattttctgt tcgtatctct ttccacgtat tatgtttttc
     4501 cacattatca tctaataaag tggccatgaa atttaaatgt aaccctactg caggtacttc
     4561 atcaataact acaatacctt ctcgatctgc taatcgcata atttcttctg agtaaggata
     4621 atgtgatgtt ctaaaagaat tagcacctat ccatttcatt aaattaaaat ctttaatatt
     4681 tacagcctca ttcaaaccac gtccattaac atacgaatcc tcatgcttac caaatccttt
     4741 aaaataaaaa ggtttattat taatcaaaaa tttgccattg gtaacctgaa cactgcgaat
     4801 accaaattct tctgtatagg tatcgatcac tttattatca tcaattaaac taattttcat
     4861 actatataaa taagcgtcta gaggttccca aaaaattgga ttcttaagct gaatcattcc
     4921 tttctttcct tcagcttcag ctacaagttg agcctcttcg tcatatatcg ctattaagat
     4981 ttttccggtc cattctgtat cgatattgaa ttctacactt gcatccaaat cagttattct
     5041 tgtattaata gtaatatcac ttatatagtc tttcggagtt gtatataact taacaggtct
     5101 atgaatacct gcataattga agaaatcaaa attaactaag ttttcctttt ttatatcccc
     5161 attttcctgt actgtttcct tcataatccc cacaggtaaa gtactatcgt caataatatt
     5221 gtttacagca actgtaattc gattattccc gtcttttaca aaccgattaa tttcagactc
     5281 aaatggtgta aacccacctt tgtgttgagt aataaacttt ccattcaaaa aaacttttgc
     5341 ttcatgtgtt gcagatccaa aacgaagaac taatctttct tctaataatc ttttaggaag
     5401 agtcacttct ttttcatacc acacccatcc aacatgattt ctaatgttct ctgtttcaaa
     5461 taaatcatta taagaagagg gaactgaaat cggataactc tctttttcat ttattggttc
     5521 taaagcaagt tcttcctgta agtcatcatt tgtatctaat ttaaatctcc agagaccgct
     5581 taagtcaatt acttgcctcg tctctgtcat aatgggatac aacatataaa ccactcctat
     5641 ctaaatatta aataaatgta tcctttaaac cacaataagc atctattgga cttactccct
     5701 taaattcaga aagacccatt agtttgtcca ccaatatttc taaagtttct tgttttccat
     5761 cataagtatt aatataagtc tttacttgag gtacatcagc caaatgaaac ggacactgta
     5821 cagatacaaa aattgttgga atttcatgaa tgtaaaacgg tatatccgga gttcctttac
     5881 ttgctggcca gacaattcgt tgaactgtgc ctggattaac attagctata ttaatgatca
     5941 agtcatattt tttagttaaa tttttaattg gtctcttagc agcatatatt ccagctatat
     6001 ttttaatttt ttcttcttgc gataatcctt tcaattcttc ttcaggtgaa atgtattttt
     6061 caacttcaaa tccttttgct gacaaaatct gtttcaataa atcaatggct tgaggtctat
     6121 ttccagcaac tacttcacca aacccacctt ttaccccatg tacatctact agtaaaatcc
     6181 ttttatattt ttctggtaca ataggccaaa tatcttgttt atctttaact aatgtaattg
     6241 cttggtcagc cacctcttta aaaattgatt tattttcaat taaaccaatt ttttccagag
     6301 cttcttgctt aggtaataaa atttgttctt ttgctttttt gtgtaatccc aatttagctt
     6361 ttaaccctaa aattcttttt aaggcatcta ataaccgatc atcagtgata attcctttac
     6421 gatagccttc catcatataa ttaaagtctt catctggatc attaaaaaat agaaatagat
     6481 cacatccagc tgcaatagaa gtgggcaata attctgatcg tttcattgcc ccagtcattc
     6541 ctaacatatg actagcatca gtaactacta atccattaaa tccgagtttt cctctaagta
     6601 agtcagtgat taattctttt gataaagttg caggaagtaa atcttcatct ttcagatcag
     6661 gattaaaatg ttttgaataa gctggcatat ggatatgacc tgccataatt gaaggtaatc
     6721 cagcttctat taagcctgag tatacttttc caaaagaatt atcccattct tccgtaccta
     6781 aactattaat actaaaagat agatgctgat ctcgttcgtc aataccgtca ccagggaaat
     6841 gtttagctgc aggcaaaatt ccactctcct gaataccttt catataagct aaactaagtt
     6901 ctaaaacttt atcagggttt tgtccaaaag atcgactaga aatgatagga tttctccaat
     6961 ttctattaat atctacaata ggagcaaaac tccagttaca tccaatagcc gatgcttcaa
     7021 tcccagaaac tctccctaat tcataagcat atttcgcatc atttgttgca ccaattttta
     7081 cttctacacc aacttcagta ccatctgtac aagctccgtt accacctgct tccgtattag
     7141 cagcaattaa tagaggaatt ttactttctg tttgtaagat atagttttga tcatatacct
     7201 catcagcagt tcctggatta tatcgtacag ctccaatatg gtactgattg accatattac
     7261 tcaagtaatt tttatcacga ctagatccca tattaacaaa caattgacct attttttctt
     7321 ccaaagtcat agaatcaatc gtctcttcaa tccaagaaat agcatcttca tctaaattat
     7381 atggtttttt tcgtaaatct acttttatag tcattttctt tctcctctac ttattagtaa
     7441 gatttttcca aaaagtatct atatattttt gttcaatatc tacataatct cccgataaa
//
LOCUS       DACEDM010000106         7443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEDM010000106 DACEDM010000000
VERSION     DACEDM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.110"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            244..1722
                     /locus_tag="IWO50_002657"
     CDS             244..1722
                     /locus_tag="IWO50_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0359726.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1999..4059
                     /locus_tag="IWO50_002658"
     CDS             1999..4059
                     /locus_tag="IWO50_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359727.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4142..4711
                     /locus_tag="IWO50_002659"
     CDS             4142..4711
                     /locus_tag="IWO50_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0359728.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4886..5692)
                     /locus_tag="IWO50_002660"
     CDS             complement(4886..5692)
                     /locus_tag="IWO50_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359729.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5830..7344
                     /locus_tag="IWO50_002661"
     CDS             5830..7344
                     /locus_tag="IWO50_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0359730.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2676 a         1218 c         1449 g         2100 t
ORIGIN      
        1 tttttgtggt tgtttacgca aatggcgcaa atcttgtaca ttttaacaca ataaaacaaa
       61 aaatatagaa tatttcctca taaatttttc cattttttgc aatattctca aaaaaaagat
      121 aaaaaatgag aataactgat gaaaaaacaa gcataatcca tcataaaatc agtataggtg
      181 aaaagatgaa ctttcatctg catatcaagt aattggtatg aggcattttt tgaggtgtag
      241 gatatgaagt tggattacct tatagaagag gaagatagag aaaaactgct gctagctaga
      301 tatttagaat ttgagaaaaa gaattatgta gtgctgccta agttatatga aacattaggt
      361 ttgtctaaat ttaaaacaag aaattatatc atagaattaa ataaagaatt tactgaattc
      421 aaaacaaagc cagagatcca aatttcagaa gaagcagaga tccatgtcga acgtctgaat
      481 ttgacggata tcaaaaattt aagaagaatc tactttcaac gatcaaaaat tgctcgactg
      541 ttaacggaga tcgtggattc aaaagtaacg atcgaaaaat ttgcagagaa tcatttttta
      601 agcatatcaa gagcctatgt aaaaagaaga gaattagtct cttttcttga acagcatcaa
      661 ataaagttaa agaaaaacat attagttggg gaagaaataa acatacggaa cgtattattt
      721 ggggtatatt tcggggtctt taatggtttt tctatgccat ttagagaaag cgtcatgaat
      781 gaagcaaaga gtatcgtgca atattttgaa tatctgttca atttaaaact gtctgcaaca
      841 aaacgaatca aattcaattt gctaaccact attagtttgt tgagggccta tgggaatcaa
      901 ccaatcggaa aacgtttctt ttcagatgaa tactttaaga gctcagaagg aatgaagatc
      961 atcaaggatt tatcacatat ttcttttctt catatcccta aagagcagat cattgatgag
     1021 atttcttata tcatgttgtt tttgatcacg gaagaagaat ttgaattaga tggcgcattt
     1081 ggacttgcgg aagctcattt tcatgatatc cacatgatta gtcaaaaaat gtcagtggaa
     1141 ttgattgatc aattaccatt ttcgattgaa atctctgaag aagaaagact atatatacga
     1201 aaagaacttc gtatgggtat gatgcatgtc aatcgaaaaa attggatatt cgactttgag
     1261 atgactagct ttacgtctaa aaagcagcta cagtttttct tcgagacgta tccaatattc
     1321 agtacagcaa tccaaaaggt catacaagag aataaacgct atttcttggg aaaagatgaa
     1381 cgtttcatga ttcgcttatt ttatgattat ctctttttat tggtcggtat ttgtccggta
     1441 tcatattttg aagcaccgat tcatgtctgt attgattttt cgcaaggatc tgcttatacc
     1501 gattacatta tttctcaagt gaaaggattt aaaaattaca atattgtgat tgagagtcga
     1561 atcacttctc gtacgaatat ttatttatct gattgtttac ttgaaaaatt taataaagaa
     1621 cagataattt ggaaaaatcc accgactcca gatgactggg aatattttgg aaataccatc
     1681 atcagagaaa aaaatagaca tttaactgaa aaaatcagct agtatttatg gatggcagct
     1741 ttgtctcaca tatttattag ctgatttatg tttttttcaa agaaaaggta actagatata
     1801 actagatata actagatata ttacttaaca aaattaaaaa gtacataagt tattattccg
     1861 attttcttga aacaataaaa aatcaaaaaa aatactctcc taatagtcca tcttcttttg
     1921 attttattta tataatgaaa tcatcttatg aaaattacta cttcatgttt agacaagagt
     1981 agggaggact aagaataaat gtgttcaaaa agacgttcaa aacaactaaa taaacacgct
     2041 tgtcgtttag ataaggaacg gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc
     2101 acgatgctga tcagtttatc tgtaggtgtc tatatgcatg tacccgttca ggcaacgaca
     2161 caaaccatca aggtcgtacg tacctctgtt tctgagacca ctgattcccc tatcactatt
     2221 actcatactt tcactgatgc aaatggaaaa cagctacttc ctgcattgac ttcctataat
     2281 aaagtcagtt atcctccaac gataaaccac tactggttaa ttcctaacaa aacattatgg
     2341 acatcggaaa ccggaagcag tgtgccgctt tctatctttt ccgatatgac tgatcctaca
     2401 aaaggtgatc agttaaaaca gatcactgat attttgaatc atgctttgac agatataaag
     2461 gacggtggaa gcgcacaagt ttcttatgta tatgaaaaag attattcaca gtttgaaaga
     2521 aagaaaatcg atgttctggc agggacaaaa gtttcgatat ctgatctggt agaaaggctg
     2581 aagaacgcag atggaacaca aggagatatg tccaaagtaa tttcaaacaa tgggaatatc
     2641 gtggatacga ctaatacagg aacatctact atttccttgt cctattttga tccaattgct
     2701 ttaaaaacaa tgactgcatc tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa
     2761 ggaaaagatc ttcacttaag aacaggagag agttacgccc ctaaagatct tgttgaatat
     2821 gtaacaaaaa gcgatggaac ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta
     2881 gatactacaa ctccgggaga atatcccgtt gtattaactt atatagactc gaaaacatca
     2941 aatgtggcag aaacaacagc tgtagtttat gtagaagcat ctgctgcttc atcgactgtc
     3001 atcactcatt ttatagatag tcaaacaaat aaagaacttc aagcagaaac acatatgagt
     3061 agtgtaaatc gtattcctga tttgcgccta cctgctgaat tttctgatcg agtgccaaat
     3121 atagatttat cgacttatga acaaaatggg aaagtcactt ctatttctga ttggatgcaa
     3181 gcaaacggat tagacagaaa aagtcactgg tcggagatcc ttgaagtact tcactcagaa
     3241 ctgatgaata ataggaagca atcaggaggg gaaattacct ttacttatgt ttatatacca
     3301 gacaatcgtt cattagaagg tcatgatctt attgttcatc caaacgaaaa aataaacaaa
     3361 gaagagttga tccaatcagc aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta
     3421 tcggttacaa tcaatgaagt accattggag gatggttatt tgaccaaaca aacaggtgat
     3481 tttacgatta tgtatagtta tcatgacccg tattcactcc aagaactcca tgctcagtca
     3541 ctattacatg ttttaccatt aaacaaacag atagatcaaa aagaacaaaa aagacaagaa
     3601 gaaagcttgt cagaaaaaaa acaacaaatg gctcatgcag tttctccaac agggaaagta
     3661 aaacaaacaa gaagcctaga gttagcaaga atatctagaa aaatctcaga atctaataaa
     3721 acgataaggc aagaagctca tttggcccca caagtttctg gattggaaaa tgaaatttta
     3781 gagcctaaaa taaaacaaca atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag
     3841 aaggaatcag atgaccagaa ggaaacgaag aaaaaacgct tgtcaactat tgattccacc
     3901 gatgagcatc tgcagccgca agtaccactt cctagtccgg gaacacccgc acctggtgga
     3961 ggtattcctt cttctgcttt aggagatttc atgcgaggta tggcaggaac atgggtatac
     4021 gctaatcgaa atgaatttga cgcagatgac tggttctgat ttatatctct atctgttata
     4081 gtcaaataag tagctgagtc atatgttatt tggcataatg gagacgagga gggatagaaa
     4141 aatgttagat aaaacgttac cttacgcaga aatctggatg acaagaccac ttcaacaaaa
     4201 tgttccaatc acgcctttag cggaaggttt tgaatttcga ttttatcaaa aagaagatga
     4261 atctgcttgg gcggagattg agacttccgt tggggaattt caaacgacag atgaagcact
     4321 tgagtatttt caacaaaact ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt
     4381 tgtagagaca aaaaaaggag aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag
     4441 agagcaagtg tatccggttt ttcattggtt agcagtgaaa ccagattacc aaggaaaagg
     4501 gctcgcaaaa gcattgactg ccagagtctt gcaagaattt cctaccctcc atacggacgg
     4561 accaatctat cttcacacac agacatggag ccatcaggcg attgctcttt ataaaaaaat
     4621 gggttttact tttattgctg aaaatctaga cggcacacct aatccagatt atgaaaaagt
     4681 tatgcaggtt ttaagtaaat tagataaata acgaaatggt gaaataagtc ctcagtattg
     4741 ggataaaaaa cgaaatagtc attcctcata tcttactttt ttgagttaat actatcggat
     4801 aactaatggc atgccataca ttcaaaaaag aaggggcagt gacaaaagta ttgtcactgc
     4861 cccttctctt gttttttagc atgttttatc gtttgatttt tttaggaata aaattcttga
     4921 tgaacgtaaa tttttggtaa tcgtttcgac tatagaggta accaaataca ctgaaaacaa
     4981 ctgctccaat aaaattaaca aataaatcct tcatagtatc catgattccc acgtctaagt
     5041 aaccattttg gatgattgtt ttatctgttt taccatctgc attttgactt tcaatgattg
     5101 tccgagtgat atctggaaca tgatagacag tgtttccgtc tttacttaac tccacactat
     5161 tgatttgctg gatgatcgta tctttttgca tatcgaattg cagtaattta tctgctgaaa
     5221 attccacaaa ctcccacata acaccaactg tcatcgaaaa acagaaggaa acaactgcta
     5281 gtaataaagg agtcaagttc atggtttttg tatttttatt gagcaaataa acaagggaaa
     5341 agccgacacc cccagctaaa aatccgctga atgcatgtag aaaagtatcc cacattgaaa
     5401 aatagccata aaaatcactt aactctccta aaactgttga actaaaaata aaagataaca
     5461 aaatgatttc tagcatattt ggaatctgta tctttaatat cttttccaga aaataaggga
     5521 tttcaaataa aattaatgtc agtaataaaa gcattgtatt tccccagtgt tggttccaga
     5581 cctgatccac tagcataata acgacgatga tacgtaataa aatatagatt ccgtttgtga
     5641 ttctacctgc agtttttgta ctgttcgtaa aaaaggtttt caaatgattc ataaaataac
     5701 tccttcatgt atatccttaa ttatactaag aagcgtgtgt ttaccaaagc tatatgactt
     5761 aaagaaaaac agagttgata ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg
     5821 gggatacaaa tgaaaaaatt agttgctggt ttattaacag ccgttgcact tcttgctatg
     5881 agcgcatgcg gcgcagatca gaaaaataca gcgcagacaa gttctagtac gaaggaatct
     5941 gccgaagtat catcaggcgc ttctgaacaa gtatatacag atccaaaaga aatgaaagaa
     6001 tcttatgatg tcgtgattat tggagctggt ggtgcaggga tggcagcagc tatcgaagca
     6061 aaagatgctg ggttgaatcc agtaattttg gaaaagatgc ctgttgccgg aggaaacaca
     6121 tctaaatcat caagcggtat gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc
     6181 aaagactcga atgatgcctt ctatgaagaa acgctaaaag gtggtggagg aacgaatgat
     6241 aaagaaatgc ttcgatactt tgtcgatcac tctgctgatg cggtcgattg gctagatcaa
     6301 aacggtatca aactggacaa cttaacgatc actggaggga tgagtgaaaa gaggacacat
     6361 cgtccgtctg atggctcggc tattggtgga tacttagtgg aggggttatt gaataacgtc
     6421 catgagcggg aaatccctat ttttgtcaac gcagatgtga cagacattaa gaaaaatggg
     6481 gaaacggtca atgaagtgac tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga
     6541 aaagcaatcg tagttaccac aggcggtttt ggtgccagca aagaatttat tgaaaaatat
     6601 cgtcccgatt tagctgatta tattacgaca aatcaagaag gatcaactgg agatggtatc
     6661 accatgattg aaaaattagg tggacaaacc gttgatatgg ataaaataca aattcatcca
     6721 actgtgcagc aggatgaggg ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc
     6781 ttggtcgatc aagaggggaa aagatttgtc aatgaaatgg atacaaggga taaagtatct
     6841 gcagcaatta cagcattgcc tgaaaaatca gcttatttga tttttgacca aggcgtgcgt
     6901 gatcgtgcca cagctattga attttatgac caaaaaggat atgtcacaac tggaaagaca
     6961 atggaagaat tagcagaaaa aatcgattta ccaaaagaaa cactagcaaa aacagttgat
     7021 ggatggaata cggatgttgc ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg
     7081 gaacatgact tatcgtctcc gaattattat gcgatcaaaa tcgcaccagg aatccattac
     7141 acgatgggcg gtgtgaaaat caatacgaac actgaagtat tagataaaga aaaccagcca
     7201 attagggggt tatatgctgc tggtgaagtc acaggtggct tacatggaaa caaccggatc
     7261 ggtggaaatt cagttgcgga gatcattatt tttgggcgac aagcgggcat acaagcagct
     7321 cgatttgctt cggaaaatga atgaaataga acgtaaataa aaagaggttg tgaaatcagc
     7381 gttttgagct aataccgagg gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa
     7441 aag
//
LOCUS       DACEDM010000107         7397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEDM010000107 DACEDM010000000
VERSION     DACEDM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.112"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IWO50_002662"
     CDS             complement(<1..142)
                     /locus_tag="IWO50_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0359731.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IWO50_002663"
     CDS             complement(183..437)
                     /locus_tag="IWO50_002663"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0359732.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IWO50_002664"
     CDS             complement(548..802)
                     /locus_tag="IWO50_002664"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0359733.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IWO50_002665"
     CDS             complement(995..1270)
                     /locus_tag="IWO50_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ0359734.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IWO50_002666"
     CDS             complement(1242..2195)
                     /locus_tag="IWO50_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0359735.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IWO50_002667"
     CDS             2807..4300
                     /locus_tag="IWO50_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ0359736.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IWO50_002668"
     CDS             4435..4731
                     /locus_tag="IWO50_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0359737.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="IWO50_002669"
     CDS             complement(4755..5714)
                     /locus_tag="IWO50_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0359738.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="IWO50_002670"
     CDS             5897..6748
                     /locus_tag="IWO50_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359739.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..6981
                     /locus_tag="IWO50_002671"
     CDS             6769..6981
                     /locus_tag="IWO50_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359740.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DACEDM010000108         7184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEDM010000108 DACEDM010000000
VERSION     DACEDM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.113"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            33..1367
                     /locus_tag="IWO50_002672"
     CDS             33..1367
                     /locus_tag="IWO50_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359741.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            2003..2500
                     /gene="dfrG"
                     /locus_tag="IWO50_002673"
     CDS             2003..2500
                     /gene="dfrG"
                     /locus_tag="IWO50_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0359742.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2572..4524)
                     /locus_tag="IWO50_002674"
     CDS             complement(2572..4524)
                     /locus_tag="IWO50_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0359743.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4531..4827)
                     /locus_tag="IWO50_002675"
     CDS             complement(4531..4827)
                     /locus_tag="IWO50_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359744.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            5005..6273
                     /locus_tag="IWO50_002676"
                     /pseudo
     CDS             5005..6273
                     /locus_tag="IWO50_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6448..>7184
                     /gene="msrA"
                     /locus_tag="IWO50_002677"
     CDS             6448..>7184
                     /gene="msrA"
                     /locus_tag="IWO50_002677"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0359745.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKR"
BASE COUNT         2364 a         1159 c         1229 g         2432 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttactc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa aaatacacag
     1441 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
     1501 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
     1561 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
     1621 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
     1681 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttta agtagttcag
     1741 ttttggagta caaaatgaca aaatgtactt caaaatggac tacttttttc attttaactt
     1801 acataccaaa ttacatacaa aatgatttat aaaatgcaat tgaaaatgat aattgaatga
     1861 aaatttgtga ttttgtggta ttatgataat ataacttata gaaaaaattg cttattcaac
     1921 taacgggcag gataatgaga aaaggttttt tgatagaaat aagaaattag atggtaaatt
     1981 agttaaaaag gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg ataagaatag
     2041 agtgattggc aaagagaatg acattccttg gaggattccc aaggactggg aatatgttaa
     2101 aaatactaca aagggacatc cgataatatt aggtaggaag aaccttgaat caatcggaag
     2161 agccttacct gacagaagaa atattattct gacgagagat aaggggttta cctttaatgg
     2221 ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat
     2281 ttttattttc ggaggagaac agatttataa tttgtttttc ccttatgttg agaaaatgta
     2341 catcacaaaa atacatcatg aattcgaagg agatactttt tttccagaag tgaattatga
     2401 ggaatggaat gaggtatttg cccaaaaagg gataaagaat gataaaaatc cgtataacta
     2461 ctattttcat gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa
     2521 gaacagtaaa gataaagcac aagagcttgg gattactcca agctcttttt gtcatttgaa
     2581 ataaattata tctttccaat caaattgttt atctgtaatc cctaatcttt gagcaggtgt
     2641 caaaaccttt ttatccgctg atttaaaagg gcgacaaaag ttgtagtaag ttcttagaat
     2701 tgtaatcgca aattgagcat acttcggatt gaagttagcg taaatatagc tcttcttatc
     2761 accacgagct gttgttaatg gtctttcaag agatgatata taacgtctta tctgttgaat
     2821 aaagctattg gtcgaatggt cattaacatt taatagcatc tttgcgattt cgtttggttc
     2881 cagagcagat aaatccgttc tacaatcaac tgaataaaat cctttatcct tagtggctaa
     2941 tggatgtttg atagggttat cggcatattt gcgatatctt tctccatctt tactaatagc
     3001 ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga taatgagatg ccaaagtccg
     3061 tagaaacttt gttttgaatc ctctgatatc tccccaatcg agcagttctg cttttgcttg
     3121 tccgaattct tttaagcatt gttttctgct tttcgtcttg ttaacttggc aaataaaatg
     3181 atgtgcatcg ctcaacctta attctttcgt gaatactcta taaaaggcgt ttctaataga
     3241 gaagtcatca tcactaatca ttcgccattc gtcggcatta accatttgtt taattaacca
     3301 atagtgagca gttgtagtat aaggagcatc aacatttaaa ccatcgacaa atctactcct
     3361 attagtaatt ttccctaatt cgtgaagata agcattccta ttttcgctat cgttagcgga
     3421 tggttcttgt ggataataag accaatctaa cctatcattt ttacgactaa aagtattgag
     3481 atggtcttct ttgaattttc ttgtatcttc atttaattca tccattgaaa tattccaatc
     3541 ataggcaaca tcagaacgga acacatatct tgataagacc tcagcagata caactatata
     3601 cgtttgcata ttaaggtctt caaaccccgt atatttcttt gaaccttgtc ctttcttacg
     3661 gacgttattt aggtagtagt gcatcttgtc cgtattaagc cacatctcgt taaatttctt
     3721 tgtttgaagg ggttgtgtct catatcgttc taaaaactcc aaacaacgcc tatacagcca
     3781 ctccagctta tgatagtagg ttccaactcc aattcctaaa atatcacaag tacgactgac
     3841 tgaaaccctt ccaacaacca tcttagcgaa cattggaaga atggtgttct tttgttggtg
     3901 ataagtagtg gtttcttctc tttttggtag aacattagtg aacttcttac acgccttaca
     3961 ttgatagcgt tgagatttgc ccctactttt cccccttttg taaaattgct tcggctcatt
     4021 gaatggggtt gattcttctt ccgagcagcc ttctttatga aattgataat ctggctcaac
     4081 atcttggatg ctattgattt ctataaggcg tttaatttct tcaacaacag accaattaga
     4141 taaaggggtt actgtacagt tttgcgatac acctctgcca ataggattgt cattgcagta
     4201 cataccttta tgacctttat ctttggaact accagtcatt ttatatcgac ttggtttccc
     4261 ttttacatct ttgaatttgt gctgttcttt cccaaaatta caacaaaacg gattgatgca
     4321 gtaattatat tgaatgggat attcagaacc gttccattta aaagacgttg gcaagaacaa
     4381 cattttgtta taccttgttg caaatcgttt tggagttaat aaatcatatt gtttagccct
     4441 atcttttaat tcttcgtttg aaatcggaat tttaacatct acaatttcat cttccttcgt
     4501 ggctaatctt ttaagcttgg tcattgtcta tcaacccact ctatctttga tacccatttt
     4561 ttcaacaatc cgcttattac ttcgcttgtt ggcaatccat tcaataaact tcttatcgtc
     4621 taaaatgttc aataagaatt catccactgc ttcgctttca gtacgctctg catattcagc
     4681 gtattgctta actatcaccc ttacttgctc tgatatttcc caatccacag acttagcgtt
     4741 cttatttttt ggctcaagaa attgcatagg aaaatgcacc cctttttgct tagtaaattg
     4801 taaccatttt aacacaccaa tttgcattcc tacaataatt atttcacgtt cgcattttta
     4861 tatacatttt aacttacatt ttgtggtgta aattaacaac aaacgttgat ttaacaataa
     4921 aaaacgagta tcaaattcag ttgcaatttg atactcattt aggtttacat tttcactttt
     4981 tgtactccaa aactgaacta cttattttta tttagttgtc tctatatttt ctctgcttat
     5041 cggacagtag acatctacta tcgttccgca attgccgaag ctatcgcaat aacacttgtt
     5101 atccctgtat tattttatgc ttatcaaatc atctctggaa aagaagaaaa gtatccgtct
     5161 gtaaagctag cattgagtat gagtttgcta gtgtacagcc atgtactatc aacattaatg
     5221 agtacagcat taattatcat ttttattttt attcgtttgg tttctaaagg tttcaagaat
     5281 gcagatttta ttgctatatt taaaaaatta tttagtgcgg caggaatgac attggttctg
     5341 acgagtgctt tttggtatcc gatgtttgaa caaatgctct accaaaaaat caacaaacct
     5401 agcgtgacaa atttatatgc tcatgcatct aatgtgttcg atagtctgac tgaagctatg
     5461 aacaacgact taactactta ctcaatggga ctagttggtc tgttatcttt atgtattccc
     5521 ctgattttat ttaaaaaatt aacaaacata gaaaaaaaga tttattatgg tacgtgtttg
     5581 acttggctag ctacaactag tttggtgcca tggtatcttc tgcaaaatac gccagccaaa
     5641 ttgttacaat ttccttggcg gatcttaagc ttgcaaataa tattctcttc tctgatatta
     5701 acgatgatat ttttcaaaaa tagaagatac aataagacaa gagaactatt ttatttattt
     5761 atgactgttt tgttattaat cactttaact gtttctagta aaaaaaattt caatcaaaaa
     5821 ataatttctc agccgggaca tattgttgta aacaaagaaa cagtagaggc gtataccact
     5881 aaaggaatgg attatttttt atatgattat tctcctatcg cttcattgga gaataaggag
     5941 catataataa aacacgaagc aaagattggc agtcaatggg taaaccttcc ttataaaaca
     6001 actggtagtt ctatcatata tagagtaact aatgagaaga gccaagtaat cacacttcct
     6061 gtatatgctt atttaggaac gaatgtagaa ataaatcatc aacctgtaaa ggagcaggtg
     6121 aatgctaatg gattggtaca aataagcgga gtcaaaggag ttgctgatat taaagtaagc
     6181 acaagttata cagtaacgac tattatttct tatcttgtta cattaactgc atatttatgt
     6241 ttatttttta aaatagtttt ccataaaagg taaattaact tttcatttat aaaataaatt
     6301 gattaaaaag gagaatctgt cgataaggat tctctctttt ttatacgaaa aatctgtagg
     6361 gggatgaagt aatcgcttat ttcctcactt tatgataaat tacagctaaa gtcactagct
     6421 tgtcttcttc ggaaaggagg agaatctatg gatgaggaac aaatattcat tcaaacactt
     6481 tataatctga ttttgaatcc gaatacacga gattgggaac gaaaagtatt gattcagaca
     6541 aagaatgaca taagagaaaa tatatccgtt aaagaacagt tatcaaaact agaagcaaca
     6601 ctacgtccat tagctatccg aatgaatctg acgcctgatg taatggactt ttatttattg
     6661 attacggaag gatttgataa ggagcaaaaa tatgactttt ccaagcatgc catgcaagat
     6721 gctgactatc aagaacgtgc agtatttgca gggggatgtt tctggtgtat ggtcgagccg
     6781 tttgaatcga aaaaagggat cctatctgtt ttgtcaggat acacgggcgg acatgttgaa
     6841 aaaccaaatt atgatcaagt aagtggtggt tacacaggtc atgtggaagc tgtggagatc
     6901 atttatgaca caagagaaat aagttatagc gagttgctga caatctattg gcaaattact
     6961 gatcccacag atacatttgg acaatttcaa gatagaggga agcaatatcg tccagtgatt
     7021 ttttatcaag tcgaacgaca aaaagaatta gcggaacaat caaaacagaa actagacagt
     7081 tcaggaactt tccaccaacc aatcgtcaca aaaatcgaac cagccggtac gttttggcca
     7141 gcagaaaact atcatcagca attttacaaa aaacaaccga aacg
//
LOCUS       DACEDM010000109         6114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEDM010000109 DACEDM010000000
VERSION     DACEDM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.114"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..2087)
                     /locus_tag="IWO50_002678"
     CDS             complement(12..2087)
                     /locus_tag="IWO50_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0359746.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GNFKQKFWLSLILAIPIILFSPMMGMEFPFQVTFPGSDWLVLILATILFIYGGQPFLS
                     GAKMELKQKSPAMMTLIAMGITVAYIYSVYSFIANLLNPHTHVMDFFWELATLIVIML
                     LGHWIEMNAVSNASNALQKLAELLPESVKRLTKDGKEETVSLKEVNEGDRLIVRSGDK
                     MPTDGVILKGETIVDESAVTGESRGIKKQANDKVIGGSINGDGTIEIEVTGTGENGYL
                     AKVMEMVRKAQGEKSKLESLSDKVAKWLFYVALIVGILAFIAWLFLTDLPNALERMVT
                     VFIIACPHALGLAIPLVVARSTSIAAKNGLLLKNRNALEQANDLDVIMLDKTGTLTEG
                     KFTVTGVEVLDDAFNKNEILQYLGALEANANHPLAVGIMNYLKEHEIKPYQAENLKNL
                     SGVGLEATVKKQQVKIVNEKEVERLGLNVEQALLKPYQEQGNTISFLILENHLAAIVA
                     LGDVVKTEAKEFIRTLKERKITPVMLTGDNKNAAQAVADYLGIEEYYGGLLPDDKEAV
                     VQKYLDQGKKVIMVGDGINDAPSLARASIGMAIGAGTDIAIDSADVVLTNSDPKDILH
                     FLDLAKQTRKKMIQNLWWGAGYNILAIPLAAGILAPIGIILSPAVGAVLMSLSTVVVA
                     LNALTLKIK"
     gene            complement(2107..4293)
                     /locus_tag="IWO50_002679"
     CDS             complement(2107..4293)
                     /locus_tag="IWO50_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0359747.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4293..4502)
                     /gene="copZ"
                     /locus_tag="IWO50_002680"
     CDS             complement(4293..4502)
                     /gene="copZ"
                     /locus_tag="IWO50_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0359748.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4515..4955)
                     /locus_tag="IWO50_002681"
     CDS             complement(4515..4955)
                     /locus_tag="IWO50_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0359749.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5029..5568)
                     /locus_tag="IWO50_002682"
     CDS             complement(5029..5568)
                     /locus_tag="IWO50_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ0359750.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1597 a         1373 c         1070 g         2074 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatctga
     1981 ctatggtcat gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact
     2041 acttcttttt tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc
     2101 cttctcttac tttttcatgt gtcgatttaa gctcagggag ttcaacaata cactgacgga
     2161 actaaacgcc atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg
     2221 aataccaatc gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt
     2281 caacctagac aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac
     2341 atcggctgtt tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa
     2401 tgctaaggcc ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt
     2461 ctgaagtttt tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt
     2521 tggatcaata ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag
     2581 cataaaaaca tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt
     2641 gatttgatca gccacggcaa tccacccgat cacttgcttt tcatcagcta aaaacatgac
     2701 tgtttttccc tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt
     2761 caattcaatt aatcgtttac gtgtacccgc aaaatagcgt actccattaa tcgtaccgct
     2821 gatcccagca cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag
     2881 ttttccgtat tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata
     2941 aaaaatagat aggacttctt ttgaaccgac tagatctgta actttgggct ttccttctgt
     3001 aatcgttcca gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc
     3061 tccgccttta attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt
     3121 tggggtcgct aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat
     3181 caatgcgagt tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat
     3241 gactaatacg atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc
     3301 tttgctccct tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc
     3361 gacttgtgtg acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt
     3421 gtcatccgga ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc
     3481 actgcttcct gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag
     3541 ttgatcacca acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac
     3601 ttgggcggtt tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt
     3661 tgctgtatgt tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc
     3721 aaaataaaga tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc
     3781 agccgatgtt ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg
     3841 gtacgcacct ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa
     3901 ctggacgatt ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc
     3961 aatcattgac aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat
     4021 ttttttcaaa tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa
     4081 aattgcacca taaccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc
     4141 atatttgaca cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg
     4201 tccgttcagc tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac
     4261 gaacgtatcc attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat
     4321 ccattgattg cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta
     4381 ccgtttgctt ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt
     4441 tcttctactc ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc
     4501 attttttctt cctcctattg atgttgttta cattcacatt gacctgggat acagttacat
     4561 tcgatcgtat ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct
     4621 tgagtcaaag tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag
     4681 atatgggaaa aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga
     4741 tgataaatga atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacgt
     4801 cccagcaaag ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg
     4861 atttcttggg ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa
     4921 tcactgatct ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa
     4981 tgtaatcatt agcttatgaa tacagattac gcttgtaaac aaaaactgtc aattattttt
     5041 gctctctatt ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat
     5101 cagtttattt cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc
     5161 ttcaaaatgc cagataaaag ataaaaattc taagtattcc ctgtttagtt tctctgaaaa
     5221 tgattcaggc atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac
     5281 tactcggtag atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt
     5341 aatcctttcc tctaatgtag aagtataatt tccttcaaca atccatgacg gattagatac
     5401 cataaacgat tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt
     5461 atgccataaa aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc
     5521 gagggtcgat tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca
     5581 cttctctttg gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact
     5641 gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaataaat ggtgttcaat cagctgacct
     5701 tcagcattaa ctcaaaaaac ggcaaaatat gaggagctat tttttcgttt gttcctctaa
     5761 tactcaggtc aaatcgctga ttttacaacc tcttccttaa acggtgttct cctgccaaca
     5821 cctcggtcac aaggcacaat agtcaacaaa catgagaagt tgttttttga aattgctcct
     5881 tgtgactcgg agttaaacgg caggctcact acctctttct taaacggtgt tcaaaagctt
     5941 gcatctgcgg taataagccc agcaaaacga aaaatatgag gggctatttt tgtttggctg
     6001 ttcttattac ttgatgcagg acagcttttt cacaacctct gattcacggt gttccagaag
     6061 tagcttctgc gaaaaacaag ccgaaacttc acaaaaattt gagaagcaat tttc
//
LOCUS       DACEDM010000110         6036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEDM010000110 DACEDM010000000
VERSION     DACEDM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.115"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(129..776)
                     /locus_tag="IWO50_002683"
     CDS             complement(129..776)
                     /locus_tag="IWO50_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ0359751.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1184..1687)
                     /locus_tag="IWO50_002684"
     CDS             complement(1184..1687)
                     /locus_tag="IWO50_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0359752.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1810..2574)
                     /locus_tag="IWO50_002685"
     CDS             complement(1810..2574)
                     /locus_tag="IWO50_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0359753.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2682..2957
                     /locus_tag="IWO50_002686"
     CDS             2682..2957
                     /locus_tag="IWO50_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0359754.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            complement(3158..3505)
                     /locus_tag="IWO50_002687"
     CDS             complement(3158..3505)
                     /locus_tag="IWO50_002687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359755.1"
                     /translation="MIPSVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWG
                     KAPQKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFH
                     NLIDGVLSAASRY"
     gene            3673..4602
                     /gene="yidC"
                     /locus_tag="IWO50_002688"
     CDS             3673..4602
                     /gene="yidC"
                     /locus_tag="IWO50_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0359756.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            4705..6036
                     /locus_tag="IWO50_002689"
                     /pseudo
     CDS             4705..6036
                     /locus_tag="IWO50_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1873 a         1155 c         1110 g         1898 t
ORIGIN      
        1 aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg ttagcattta
       61 aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa
      121 caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc
      181 gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac
      241 gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg aaacattcga tgttttctga
      301 cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat cccttttaat
      361 ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt ccagccgatg
      421 tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt
      481 cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac gatttgcttc
      541 agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt
      601 aaaaatgtat cgatttctgg cagttcgtga tctaatatgt ctcgaatctt ttcagctgct
      661 agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac
      721 tgttctactt ctcttattat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt
      781 ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa
      841 gtatgtgtta cctgaagatc ctggactctt ttaaataaga tacgttttgc atcttatcat
      901 cacccccttt aatgcttatt attctatgta catgattcat tcgataaata aaagtaaacc
      961 gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa agtcgtgtca
     1021 aagtcccttg tttcgaatga atggtgttca aaagcttgca tctgcagtaa taagccgaaa
     1081 cttcacaaaa atttgagaag taattttcgt aaattccttc ttatttcttg aagctgacca
     1141 cttctgtcac aacctcaata tggtctatta ttggttttac ctgttaaatc gaggattctt
     1201 tcccaaagta acgttgccat ctcttacgca tacttgcaga catgggctga gccgcttctt
     1261 tgactgcgca gtatttgtcc acgaatgtca caatatatcc ttctttatac ttaggcggtg
     1321 cgatcgttgc accccacatg tgcttcaaaa tgatatctcg ctcaagatcc gacaaatcgg
     1381 tgattttttc tgcattcttt acagcaattc ttggatggat atacgcatgt gtcccctcgt
     1441 caaatttcgt cgtacgccaa tcatagtaga acagatcatg caaaagtcca gcacgcgctg
     1501 ttgctttagc attgccaccc catttttttg cgagcagata actatagtaa gaaacactga
     1561 tggaatgctc caaacgagtg gaatgaacat gctgtgtgta attcgctaat tttttcactg
     1621 cttctgtttc taaaagatct tgtacataag acatatattc ttcgtcttca cgccattttt
     1681 ctgtcataat tatcgatctg ccaccttttt gaatttcatt ttcttctgaa attgatctaa
     1741 gtataccacc agtgttcgga ttattctagt cgctctagat aaaatagcta aagcttaata
     1801 gttccttaat cataaagaaa aatgataaag aacgatccct gctgcgactc cgacattcaa
     1861 tgattccgca tttcctttca ttgggatata tacgttctga tcagtcaaag acaagatttc
     1921 ctgactgact ccctgacctt cgtttcccat gattatagcg aaatcctctg ttttttctac
     1981 tttgtctaat gcatgtgctt cttcatttaa ttctgtaccg tacaccgtaa accctttgcc
     2041 tttcattaca gggattacat ctgccaaatc tttttgtaac acaggcaaat gataattgct
     2101 tccctgcatg cttcgcagca cctttgtact ataaatatct gccgttcctt tacctaataa
     2161 aacaccagcc ataccaaacg catctgccgt acggatcatc gttcccacat ttccaggatc
     2221 ctgtacatta tctagtaaaa gccagcctcc tgaaaaatct ggcgtatctt cagatgttaa
     2281 cgtcatcact gctaagatcc cttgcggtgt aggtagatct gacatagcgg tcatgacctc
     2341 atctgaaact agaaaagtag aaatctcttt gtttacagcc agccagtctc cccattcttt
     2401 catacctctc tgtgtcacaa aaagttcttt tatttctgca tcagacgacg cagcttcttc
     2461 gatcagatgg aacccttcca aaagatattc tgccttttct tctctgtatt tctttttatg
     2521 tagttttttt aattctttga ttcttggatt ttttgctgat tggatctctt tcacattcct
     2581 tcacctcacc tgattatatc atggaatagg tgcttgagct tgtttcatcc taaaaaaatc
     2641 ggtatcattg atttataaaa caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga
     2701 atgtccaagg acgtgtccag ggcgtgggat ttcgctatat gaccaagatg gttgctgatc
     2761 agttaggcgt gaccggaacg gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa
     2821 tcggaaatga cgacatcatc caaaaattta tcgaagaagt caagaagtct ccgagcccaa
     2881 gtggacgagt gcagtatgtg gatgtccaag aagatccgat gattgaggaa cgaaagaaat
     2941 ttgatgtggt gggataaggt tgtgaaaagg tcgcttagtt ttgagtatta aggaaaattt
     3001 tgtgaaaatg gctttccaca tttttgcata aatttgactt aataccgaga aactgacttt
     3061 tgaacaccgt cgataaggtt gtttttgaag cgctttgcct cgagtattaa ggagaaacat
     3121 tttcaaaata gcttctcata ttttgaaatg taatgactta ataccgagag gcagctgata
     3181 aaacaccgtc aataaggttg tgaaaaagct ggtctgcatc aagcattaag gactggttgt
     3241 gaaaaagtcg cttagttttg agtattaagg aaaattttgt gaaaatggct ttccacattt
     3301 ttgcataaat ttgtcttaat accaagaaac tgacttttga acaccgtcga taaggttgtt
     3361 tttgaggcgc tttgccccaa gtattaaaga caggtagtaa aaaagctgat ctatcacttg
     3421 cagtgagttc atttgactcc tctatttcgg gcagaataaa aaaagttttt agttttttaa
     3481 ataagaaaga aacacttgga atcactcttt tctttccaag tatttctttc ttgatttttt
     3541 gtcttttttc aaaatttttc gctacgaaaa agaaagaata tttatatgaa atttctatga
     3601 agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag
     3661 aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc
     3721 ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcagga
     3781 atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat
     3841 ttcaattggt cttacggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc
     3901 ttgccactag gtatcagtca atcaaaaaaa acaatgatcc aatctgaaaa aatgcaagcg
     3961 ttaaaaccac aagttgaagc agcacaacaa aaactaaaag ctgcaacaac acgtgaagaa
     4021 caaatggcag cacaagcaga aatgcaacaa gtatatcgcg aaaacggatt gagtatgacc
     4081 ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtatttt
     4141 acggctcgct acacagaagg tatccgtgaa tcatcttttt acggcatcga tctaggctca
     4201 ccaagtatgg tgcttgttgc cattgcaggg gtcgcttatc tgcttcaagg atatatttca
     4261 acaatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct
     4321 gctatgatcg ttttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtg
     4381 ggtggtgtgt tcagttgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg
     4441 aaagcacaag tggcagaaga gttgaaacaa aatccgccaa aacaagtcgt cactccaaga
     4501 aaagacgtga cagaatcggc aaagccagct gacaataaac aaccaaaaaa cagtacaaac
     4561 aacaaaggcc gcaacgcagg aaagcagcaa aagcggaaat aaaaatatat gacaaaaaac
     4621 tgtagacaaa taaaaaaatt tctttgttct acagtttttt tgttacaatc aaagaaaacg
     4681 tcgcttttcg aggaggaatg tataatgatg tccaaataat caatggatag tcgaatggaa
     4741 gaagccaaaa tttcgctaga tgcacttgtt cctaaaaatc atttggtcca taaaattgac
     4801 aaaatattgt tatttgattt tatttatcca attgttgaat caacttactc gaccattggt
     4861 cgtcctagcg ttgatcctgt catcttagta aaattagtat tcattcaata tttatttagg
     4921 attcgttcga tgcgtcagtc cattaaagaa gctgaaacaa acctagctta tcgttggttt
     4981 cttgggctta gtttaatcga taaaattcct catttttcaa cgtttggtaa aaactatgta
     5041 cggcatttca gagaaaccga ttgtttgaac agatatttat cattatcctt gaacaagcag
     5101 ttgaagctag atttattcat gaagaaattt tgtatatgga ttcaacctat attaaagcga
     5161 atgcgaaaaa acatattttt acaagagaaa tggtacataa ataagccatt ttataccaaa
     5221 aacaattgaa agaaaaaatt aatgaagcaa gaattgcaga aggcaaacgt ccttttggtt
     5281 caatcattca tcaggaggtg gtagaaggga aaattagtgt cgcagatcca gaaagtggtt
     5341 attatgtaaa aacagaacaa gaaaaacagt ttgcttattc tgctcatacc gtttgtgatg
     5401 aaaatggttt tgtattagat gtcatgatta cgcctggaaa tctacatgac agccgaatgc
     5461 tcgtgccaca aatcgaacgt gttaaatcat gttgtggtgt agctgctgat gctgcgtaca
     5521 aaacaccttg gaacgccaag tatttaatcg atcgaaaact acgacccatt tttccttata
     5581 ctcgacccaa aagaagcaaa gaacgtttta aaaagaaaga tttttattat gatccctatt
     5641 ataactggta cctatgccca aatgatcaaa cacttcagtt tcgcacaact actcgagatg
     5701 gttataaaaa atatgtatct aaatctttta tttgtgaaag ttgtctatta ttgaaaaatt
     5761 atacagaaag ccagaaacac cagaaagaaa tacatcgaca tatttaggaa gactacttag
     5821 atgaagcaga acatttacgc catacggtgt acaatcgcga aaaatataaa aaaaagaaaa
     5881 gaaacgattg aacgcgtttt tacagacgca aaggaaaagc atggaatgcg ctacaccaaa
     5941 tatcgaggct tggaaaaaac caggtgcata cgatgcttgt ttttgccgca atgaatctgc
     6001 aaaaattagc catttggcag tgggggcata tgtctt
//
LOCUS       DACEDM010000111         5939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEDM010000111 DACEDM010000000
VERSION     DACEDM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.116"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(107..1405)
                     /gene="eno"
                     /locus_tag="IWO50_002690"
     CDS             complement(107..1405)
                     /gene="eno"
                     /locus_tag="IWO50_002690"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0359757.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1521..2276)
                     /locus_tag="IWO50_002691"
     CDS             complement(1521..2276)
                     /locus_tag="IWO50_002691"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0359758.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2380..3570)
                     /locus_tag="IWO50_002692"
     CDS             complement(2380..3570)
                     /locus_tag="IWO50_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0359759.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3680..4681)
                     /gene="gap"
                     /locus_tag="IWO50_002693"
     CDS             complement(3680..4681)
                     /gene="gap"
                     /locus_tag="IWO50_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0359760.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4722..5759)
                     /locus_tag="IWO50_002694"
     CDS             complement(4722..5759)
                     /locus_tag="IWO50_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0359761.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
BASE COUNT         1551 a         1255 c         1104 g         2029 t
ORIGIN      
        1 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
       61 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
      121 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
      181 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
      241 ggccagcatt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
      301 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
      361 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
      421 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
      481 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
      541 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
      601 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
      661 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
      721 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
      781 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
      841 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
      901 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
      961 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     1021 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     1081 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     1141 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     1201 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     1261 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     1321 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     1381 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     1441 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     1501 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaaaaa tgaatctgct
     1561 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     1621 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     1681 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     1741 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     1801 acttgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     1861 gttttgcctg cttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     1921 tttgcaaaga tagcttttgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     1981 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     2041 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     2101 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     2161 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     2221 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     2281 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     2341 ttgatcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     2401 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccaccac ctgttgagat
     2461 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcagctgagt ctccgccacc
     2521 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     2581 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     2641 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     2701 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     2761 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     2821 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     2881 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     2941 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     3001 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     3061 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     3121 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     3181 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     3241 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     3301 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     3361 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     3421 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     3481 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     3541 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     3601 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     3661 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     3721 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     3781 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     3841 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     3901 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     3961 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcaggaa
     4021 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     4081 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     4141 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     4201 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     4261 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     4321 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     4381 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     4441 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     4501 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     4561 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     4621 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     4681 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     4741 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     4801 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     4861 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     4921 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     4981 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     5041 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     5101 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcacg gtggttaccg
     5161 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     5221 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     5281 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     5341 ggaagtagtc ggtctagggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     5401 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     5461 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     5521 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     5581 tttttcaaaa gatctgtttc tgttcgcaat actcgttctg tgatccccat actttccgat
     5641 aaacttcgac gtccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     5701 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     5761 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     5821 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     5881 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaata
//
LOCUS       DACEDM010000112         5908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEDM010000112 DACEDM010000000
VERSION     DACEDM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.118"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(366..1484)
                     /locus_tag="IWO50_002695"
     CDS             complement(366..1484)
                     /locus_tag="IWO50_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359762.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1955..3262
                     /locus_tag="IWO50_002696"
     CDS             1955..3262
                     /locus_tag="IWO50_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0359763.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="IWO50_002697"
     CDS             3381..4580
                     /locus_tag="IWO50_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0359764.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="IWO50_002698"
     CDS             complement(4607..5875)
                     /locus_tag="IWO50_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0359765.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1814 a         1252 c         1058 g         1784 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt caacacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctact
//
LOCUS       DACEDM010000113         5874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEDM010000113 DACEDM010000000
VERSION     DACEDM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.119"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            641..1375
                     /locus_tag="IWO50_002699"
     CDS             641..1375
                     /locus_tag="IWO50_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0359766.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IWO50_002700"
     CDS             1391..2560
                     /locus_tag="IWO50_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0359767.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IWO50_002701"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IWO50_002701"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0359768.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IWO50_002702"
     CDS             3650..4576
                     /locus_tag="IWO50_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0359769.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IWO50_002703"
     CDS             4595..5845
                     /locus_tag="IWO50_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0359770.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1966 a          955 c         1203 g         1750 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DACEDM010000114         5532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEDM010000114 DACEDM010000000
VERSION     DACEDM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.120"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(254..943)
                     /locus_tag="IWO50_002704"
     CDS             complement(254..943)
                     /locus_tag="IWO50_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ0359771.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(1070..1309)
                     /locus_tag="IWO50_002705"
     CDS             complement(1070..1309)
                     /locus_tag="IWO50_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359772.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(1376..1615)
                     /locus_tag="IWO50_002706"
     CDS             complement(1376..1615)
                     /locus_tag="IWO50_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359773.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(1729..2070)
                     /locus_tag="IWO50_002707"
     CDS             complement(1729..2070)
                     /locus_tag="IWO50_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0359774.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(2057..2386)
                     /locus_tag="IWO50_002708"
     CDS             complement(2057..2386)
                     /locus_tag="IWO50_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359775.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(2977..3309)
                     /locus_tag="IWO50_002709"
     CDS             complement(2977..3309)
                     /locus_tag="IWO50_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0359776.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(4146..5186)
                     /locus_tag="IWO50_002710"
     CDS             complement(4146..5186)
                     /locus_tag="IWO50_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0359777.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
BASE COUNT         1695 a         1050 c          745 g         2042 t
ORIGIN      
        1 aaaaggtaaa aaagaatgcc tgctgcaatg aagaatagat cgtttctctt taccttgtac
       61 agattcaaaa gaaacaaaca gcctacgaaa aacgtcacta actgtgcagc attatcataa
      121 ataaataata tcgtaggagg atttaacccc accgtccata ttaaatatag aatggatacc
      181 aacagtaaca gaaaaatggc actcatttta ttgatcaatt tttctacaag ttcttgaatg
      241 tgactcactt cccttattta ttgatttttt ctacattgac taattcacac tggatcacat
      301 cgcacttttt catattattt gcttgaaagg ctaacagtgt gagtagtggt tttttacctg
      361 cttttggcaa ggataaaaag gaattgtttt ccttagattg aactacctgg ctgtaatcag
      421 aaatttttcc tcttttggag acttcatact gatacttgta atgagcatcg attagatcaa
      481 tttcttctcc ttcagataaa tcagtcatat cggataataa aacgcttggt gtatctacgt
      541 tataaccagc aagaatataa tttccatttc ccatttcagt atctagataa taagtagaag
      601 ctactcctaa actaagcgtg tcgctattcg ctccttgaaa aacagggaga ataaatcctg
      661 tagaagaaaa aacaagtttt ccagcaattt cacctttgag atccttttct aatgcctgtt
      721 tttgattagt aatcagttgt ctcttagaaa tatccgttaa actggttaaa ttatcttgat
      781 aggttttagc ctgttcctga atatggattg tttttgttac accatctact ttttcagcga
      841 ttttgtcctt ttgataacct tgataagcaa aataactaaa aagaagaaaa acaatgatta
      901 cgcccacgta aggattaaat aaagttttat tgtttttatt catctatcta ccttctatat
      961 atgaattaat ttacctacat ctaaccaaga taaaccatgt taaattttac tattcttttt
     1021 attcaataac aatgccacat cataattgat cattttcata tcataatcgt caagaaactt
     1081 ctaataatag ttggcaattg gaacctgttt ttacataagt tcgcctcaac tgttcaaagt
     1141 ttttcattga acctaacaaa caagctttct cttttcctgt tatcttcata tcattgatat
     1201 attgcatgac agataatctt ttaaggtgtt tttgtttcat catttttagc aacttttctt
     1261 ccatttcttt cgacactttt tttctgtttt cgatttcttc ataattcatg agttttccta
     1321 cctttcttgt tttttgcaaa aataaaaaag cctatgatca catacgctct acacttcagt
     1381 cttgggttat ctttttcttt cgtttaatta ctggccgtgc tggtttcaaa tccttgattc
     1441 cattatgggt tctccaataa tcgaattctt ttcgtaatct ttctttttcc atatctgttc
     1501 ctttagacag ggatttgaat acatctttag gtgaataacc acgagctaac agctgtacat
     1561 accttccttt tgtcaaagta tccatatcca tcgcctctaa aactggtcgc gccatctaat
     1621 tttcctccta aataaattcc ttactcaatg cctaaggtaa tactaccttt ttattattcg
     1681 tcgtgactcc ttctaagcct ttatctgtaa aatttattcc tccagttttt attcttcttt
     1741 atccgctaca aattcaatga tttcaacgct gacaatataa tcactcaaat atgaccaagt
     1801 atcttcaaca gataattcgt ctccattatc atccgtaagc gtcattttaa accattcatt
     1861 accttcatca tcttcaggta gcaagccgaa tccaattgga ttatcagtca atttttgatc
     1921 gtataaatca tcctcgtcta atgctaaaac atctaatcct ttacaatttc cgcctcttct
     1981 aaacgtcaca aatcctttgt aatcccaacg ttggaaagaa accttaatcg tatgtgtgcc
     2041 caatcgtata ttcggatcat aatttttcat ttgttgtaac ctcctcataa tcagttagta
     2101 ctataaaggt atctttgttt ttatccaaaa aatctaataa atcttttgta gaagatatct
     2161 cttcatcaac ttcaccataa taaatacctt cgataattgt tgctatatct gaaaaactaa
     2221 tttttactgg acatttatcg tctaccgcat tcaataccga caaaattgct tctttttctg
     2281 caatctctcg tttgaatttc tttggagcta atcgcatgac actcaagtca ttttcaagct
     2341 tttcaatcca tgcagtttta ctcattttcg caaatttttg aatcattcct ctaccacctt
     2401 tgctatctac taaattagtt ttacttcata acgaatatct ttatcgtttt tcgttcactc
     2461 aaaaatatat aaaataaatc gactcgttta tggcgtccga aacgagaaac cctagtagac
     2521 agcggtaaat gctcgattga tccaatgatc aacgtcctcg caagtatcta catgtactgc
     2581 agcacacgac acaatttctt gtgcgtcctc cccattacga gaagacctac tccttattcc
     2641 atgagtttgc gtcaaagcct agatccctta gtagctgctc atgattaatc taggcgctat
     2701 tctaacttgt ctaacttcta aattccctct tgtttactct gtgcatatta tttacacaaa
     2761 ttcatttcac cactatccaa acaagggcat ttactcgatt gaccaatttt ttacgttacc
     2821 aatcgtaaaa acctaccccg tcactccccg aagggaattg ttgatagatc aagtctactc
     2881 cttatttttc tagtgattct aagttccttt agaagtctcg acaatatttt caagcaagcg
     2941 aaaaacgata aaatacaagt tatgagaatg atcaattcag ctgattaaac gccccatccc
     3001 ttttgcggta caacgcaaga ctccattttt tcttcgacca atcagtacac cgttttggtc
     3061 ataaacatgt ttgattgttg gatatagctc ttttaaaggg ataggatctt ttaaaattat
     3121 ttgtttttgt ttcccaatgt aataaatcat ttcttctgag actttgtctt tctggattat
     3181 atatttctta cactgcacaa cggttttact ttgaccagtt gatagtttct tcgtgatctt
     3241 ttctgtagcg gtacaccgtt taccgtagtg acaaatatct tcgagactca aaattcctgt
     3301 atatgacata aaataatcca cccacttact ttaaatattt ttaacaagtt tttcttgtta
     3361 tttttcaaaa atagagaatt gattttcgtt catgacaaac aaaaaagagg gaacgcattc
     3421 aaacatgcgt tccctctaat ttcagcgttc gtcatacggc aaagagcccc acggacattt
     3481 ttataacatt atgcttaggc atagattcat cgtaatatca cgtattttta accttaacac
     3541 agccaaaaaa agtggtatca cttttgaata tctgttatac taaatccgtt acgggtaata
     3601 ccgtaacact gaaaggtaaa cgcattaagg ggtatagact taatgccacc tgtggacgtc
     3661 gacgaaggtt accagccttc cgagtctcac agggcctttc ttttttttgt attttcaatt
     3721 gtccaaattt atcgtccaaa agtgcagaac gaaaatcaat tcatcacaag catagcagac
     3781 aattaaattt aaaacaacta aaaatataca aaaaagtgta attgaatatt agcagcactg
     3841 cctgaaaaga agtaatatca ggcttttcag cttggggaaa taagtgaatt acctctcttc
     3901 atgaattatt ataactcaaa aaagcacata tgcaaacact agttccctat ttttttgagt
     3961 gaaaaataac taaaatatct aacgattatg aatttaaaac ctacgattgt gattgttttc
     4021 ataaactttt aatctaaaaa ctccggaata ctaactattt atttgtaata tttgtaataa
     4081 aattaaaatc ataaaaaagt tctaatttaa aattgatcat ttttatagta attgcttttt
     4141 atcaactact ctaaccaatt ttccgtacta actgttggta attcgttaaa tgtttctctt
     4201 tgttcgtttg caagctttgc cgcaatagct ttttcgacaa accaattttt tacataaacg
     4261 aaaattagat tctgcaaatt gtttactttt tcagtttttt gtttttgata agctttcagt
     4321 aaagtcatat ataatccttt gtcagcatca attccataag attccagttc ttcataaacg
     4381 acaatagttt gctctaattc ttcagctttt ttctttgcat tatgaatagt ttggacggtt
     4441 tgttgagctt cactgtaact tgaagaaaat gtttttatca agtttaatac attttctggt
     4501 acaaatgtat ttatactgtc ctcttgcatg atgtcgacga agttatctag caaaacttga
     4561 ttttgtaact ggtcagtttc agtgtctatt atgtgtctat tagtgtctat agaattagaa
     4621 tagaatagat tgtctgcgat tttcgcaatt acggttgagt ccttgatatg actggatttt
     4681 gccgtatttg cgattttcgc aattacggta gaatcattga tttttcgcgg ttttgccgta
     4741 tttgcgattt tcgcaattac ggtggaatca gtattgtctt cctcttccat gattttaaag
     4801 acgtcttgat tcgtaacttc tggcttgcct aagtacaata aattaggcat agattcaatc
     4861 ttaccatcac gtttatttac tcttcctctc ttctgtttca gaagacctgc tttttctaat
     4921 tcttttttga tcttagatac tttaccttcg cggcattgta acaatttcat taattcttca
     4981 actgtgaata tgaagaatat attatcatca gaatctaccc agttattttt gactgaataa
     5041 tcaaatcgat ctttaagtag catataagca atttttgcat catttgaaag atccatgtat
     5101 ttttcgttag taaaaaatac cttaggcatt tgataatatt tttcacgata aatttctttt
     5161 gatttgtaat aattaaaatc attcatagcc aatcctcttt ctgtaggata gcgttttgtt
     5221 caaaactatg ttacaatgaa ttaaacaaat atatctatcc actatatatt tttctactgc
     5281 tgataagtcg ccaaactttg ttaaagcagt agtctttttt tatctttttt tagtttacct
     5341 taaaaaataa attaaagcaa accgctctat ttcagggttt aagctcatgg taactatatc
     5401 acgatattac aataacatta tatcacgata tcgtaatatt acaatatgaa gataagcgac
     5461 ttatcagctt tgatactact gatttttata aaaaaaatat cacgatatta tgatatcgtg
     5521 atattgaatg ta
//
LOCUS       DACEDM010000115         5315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEDM010000115 DACEDM010000000
VERSION     DACEDM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.121"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            36..197
                     /locus_tag="IWO50_002711"
     CDS             36..197
                     /locus_tag="IWO50_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359778.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IWO50_002712"
     CDS             210..713
                     /locus_tag="IWO50_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0359779.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IWO50_002713"
     CDS             773..1162
                     /locus_tag="IWO50_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0359780.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..2651
                     /locus_tag="IWO50_002714"
                     /pseudo
     CDS             1149..2651
                     /locus_tag="IWO50_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            3396..5222
                     /gene="ltrA"
                     /locus_tag="IWO50_002715"
     CDS             3396..5222
                     /gene="ltrA"
                     /locus_tag="IWO50_002715"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0359781.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT         1743 a          905 c         1201 g         1466 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg gggtgcgaaa cgttcctact tgaaaagagt aggcacagca cttcattaaa
     2701 tcaatattta atgtgtgttg gagaactaaa cacacgaata gtcgaatgac ggggtaacgc
     2761 cctgaaaggc tactttaata ctctgactgg cagaaacttg agtaaacaag gctctgtcag
     2821 aagctcggtg aagtcggcag acagacaccg taacagataa tgctgacgaa agattcccag
     2881 aggtgggcgt tgtgtcctat atgccggaag tctataaaat atctatggtt agaatgtaga
     2941 tgagaggtct actgacgaat ccttgaatgt acaggtctat atcggggctg gatagaaatg
     3001 tccagtgaca caaacagtgt cgctactgtg ggtgagtaag actccgcatt tatgaaaatc
     3061 catataacgt taaaggcgtt atcaaggtag caggcttata gaggaaacct aaggatatat
     3121 gtacagatag tgtgtttgga acgttgaaag ctgacaacat ggagatttta ctgtctgtga
     3181 agtgttcaca tcagaaagtt attgtgagat tagcaatgat ataaggtgtg ttaatgtcag
     3241 tgagagtggt ggcacagtac ctatgaaacc gtgataacaa gcggtggagg gatagccact
     3301 agtcatatga agaatagaat tactaagaaa aatggtatgg gttcgagtaa gactaagaga
     3361 tgtacttgct cctgcaaaca aggagggtac agaccatgct aaagaaaaac aagctccgct
     3421 ataacgagta ttatgatatg caacatattt atgacgagtt atacgcacag agcaagaacg
     3481 gtaataactt ttataagtta cttgaaatta ttggttcgga acaaaatatt tgtttagctt
     3541 accgtaatct gaaatcaaac agtggcagta aaacggctgg aacggatgga atgacgatag
     3601 acgatataaa acaactcagc aatgctgaga ttgttgctac cgttcgggaa agccttagca
     3661 actaccgccc caaatcagtc agacgtgttt tcattccaaa agctgggagc gataaaatgc
     3721 gtccattggg gattccgtgt atctgggaca ggctggtaca acaatgtatc ctgcaagtcc
     3781 tagagccgat atgcgaacca aagttccata atcattccta tggattccgt gcgaatcgaa
     3841 gtgcccacca tgcagtcagc agagtaacga cactgataaa tttatcaaaa tatcattact
     3901 gtgttgacgt agacattaaa ggatttttcg ataatgtcaa tcatggaaaa ctattaaaac
     3961 aaatctggac acttggtatt cgggataaaa gattgatttg cattatcagc aaaatgctga
     4021 aagccgaaat tgacggcgag ggtgtcccag aaaaaggaac gccacaaggc ggtctgttaa
     4081 gtccactgct ctccctcata gtccttaatg aattagactg gtgggtaagc agtcagtggg
     4141 aaacattcca gccgaagaac cgcagtaaaa atgggtggct tcaatacgca aagaaatata
     4201 ccaaactgaa aagcggtttt attgtacgtt acgctgatga ttttaaaatc atgtgttcaa
     4261 cctatggaga agcacaaaga ttttaccaca gtaccgtaga ttttcttaat aaaaggctga
     4321 aattagaaat cagtccagag aaatcaaagg tagtaaatct gaagaaaaat tcatcagatt
     4381 ttctggggtt caaaatcaaa gtcattccaa aaggaagaac gaaacacggt tatgttgcca
     4441 aaacagatat gaaccaaaaa gcattaaaga aagcgaaaac aaatctaaag ttaaaggtaa
     4501 aagatatagc cagacatacg actggcttca atatcagcag atataatctg acagttatcg
     4561 gtatgcagaa ttactactgc atagcaacta acgtatataa caacctgaca gaagtaagtt
     4621 acgctctctt accgaccata cgaatccgtt taaggaacat tgcaaaatca gttccatttg
     4681 aaagtacaag ttcagaattt cagagcagga caaaaggaat cagaccaaaa acaaaaatag
     4741 tgatgattgc ggataatccg ctattgccga ttcaaggagt acaacacaaa aatcctatga
     4801 atttctcgca agatatttgt aattttacaa agcaagggag aaacaaggtg catgaggacg
     4861 ttgtagttgt aacaaaagaa gaaatccgag cgttgttgga aaatgaaaac cctgccgata
     4921 gcgtagagtt taacgacaat cgtatttcgg catatatcgc acaacaaggc aactgctatg
     4981 taatgaatcg tcgaggaact ccgtccactc tgatatgtat tcacaaatca gacaaaggag
     5041 ataaccttga cagatattct aatctggcat ttgtggaaat tcccattgca aaggcaatcc
     5101 taacagaatc agcagacgaa gcaaaatcac tgttaaaagg ttatgttctt aatagtcagc
     5161 agaagaaaaa actgaacaga atacggacga attacggata ccagacaata actgggaaat
     5221 aacttataaa tctatttgga tatgatggaa cgccgtatga agcgaaagtt tcatgtacgg
     5281 tgtgaagcgg gggaaaactt ggagataaca tcaaa
//
LOCUS       DACEDM010000116         5059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEDM010000116 DACEDM010000000
VERSION     DACEDM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.122"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(613..930)
                     /locus_tag="IWO50_002716"
     CDS             complement(613..930)
                     /locus_tag="IWO50_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0359782.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(931..1185)
                     /locus_tag="IWO50_002717"
     CDS             complement(931..1185)
                     /locus_tag="IWO50_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0359783.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1445..1663)
                     /locus_tag="IWO50_002718"
                     /pseudo
     CDS             complement(1445..1663)
                     /locus_tag="IWO50_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1929..2123)
                     /locus_tag="IWO50_002719"
     CDS             complement(1929..2123)
                     /locus_tag="IWO50_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359784.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            3475..4515
                     /locus_tag="IWO50_002720"
     CDS             3475..4515
                     /locus_tag="IWO50_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0359785.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT         1777 a          777 c          733 g         1772 t
ORIGIN      
        1 aatatatttt ttaccagcaa accatacata tcttttttca ttaagtatgt tcactatctc
       61 tcctccatta attatattta gcatcaaata gttattgtac ctatatttta tcccattcct
      121 tttaaaacaa gaatacgtac aaagtaaaat gttgtaaata gtctagcagg gaagtatatt
      181 ttcatgtacc aacataatat ttaagaagtt tcccatagat atattggact tcctctttta
      241 aaacacaaaa ataaatacta ccttatttag gtagtattta ttaaaagtta cgtcaatttt
      301 gattaaaaat atactacttg aacctctcat gagtaaagtc acgaaatttt tgggaataga
      361 gcgtacttga tatcttattt tttcactaat agcagtgtct cttattcacc aagcgatccc
      421 cgtagttcct aaggttctta atttttctct agactaatga ttgtatcttt agaccttcgg
      481 ccataacatg gagcaaaata aaaaggtact aagtcttata tatcaatctt cgagtaaccg
      541 acggttaaca accatatcga gcggcattaa tcacccacct gtagaggatg agcgactttt
      601 tcctaattat tgttaaaagc ctagtttcat ttgtttagat tggaaaatac gtcctacaag
      661 tatctctttt ttttcatggt cgatccgata aaataaggca tagcttttgc cgatcacgat
      721 ccgatatgtt gggatatcca ttccatagat ttcggaaact tcctcatgca tagttggaaa
      781 atttttggtt agttctaaag ccttctggat cgcacctaca aatcttttta tgctggctgg
      841 agaaagagca agctcatttt cccattcttc tattatcgtt tcaaggctgg tctgaaaact
      901 tttagaataa actagcgtgt actctttcat ctattcactc cagccgtatt ttccgaaagc
      961 ttttaccatt tcaatttctg aaactgttaa acctttctca aagtcttgga aactttcttc
     1021 aatcaatttg cgatcgtgtt cttttgctat ttgagtagta tctaatatga ataagttgtc
     1081 ttcttgtttg gcttggataa tgtctccgct ttttaatcca agagagcttg gaaggatcac
     1141 tcccacgcta ttgccgatct ttctaacctt tagttctttt cccatgttat cacactcctt
     1201 ttagtttttg atgaataaat tataacatgt tataacaaca attgccaacg gtgtttctta
     1261 agggattcca ctccatatta ttattggaca aaataagcca cttagaacgc tgaaaacata
     1321 tacaaacgat atcaaaatac gagtgaacta ctcaccatac acttccgtta acatccattt
     1381 aagtaggtat ttttattata tatagattta acatatgtcg gtcggttgat tgatatagct
     1441 aaagttataa ctcaaccgaa gccgattcat ctacacgaat aaagttgcga gtgttctcgg
     1501 ctaattcata aaaattgtta tgtgttccat atagcaatac tggaataact ttattttctt
     1561 gaatctcata agcaactcta tacacggtga ccgcatactt aaatccatta gaccaattac
     1621 ctgataaacc tccagttttt tgctcttact taaatggatc aatggcaatt tcatcacaaa
     1681 cctatagagg atgagtgact tctgcctaat tacgttaaaa aatattgaaa gtaactctat
     1741 gtagcaaaaa atatctagaa tttcatttcc tcaaaactga tcgatattgt atctaaaaat
     1801 atatactacc taaaataggt agtatatatt aaaatatgtg cttttgttag aaaaatcaat
     1861 acataaatta tcatatctgt atatgtcatt taggtatttc agttatgtta taatagtttt
     1921 gctgtctgct accccggcct aatctggcaa ggggaggagg agatataatg aaataccttt
     1981 ttgattttgt ttttgcaatc aagccacttg cagatgtagt gtgcagttac ctctgcaaat
     2041 ggcttgattg caaaattgcc aaaaaagaca gtaagcctac ccgttaattc tataatatag
     2101 aaacgtaaaa aaaaccccat cagcctgcac gctgatgggg tttggtgata taatgaaata
     2161 cctctttgtt actcataatt gtaccataat tagtcacgaa tgcaaccaat agaaaaaact
     2221 acttagaacg gtagatcatc gctaataaca atgcattgtc caccaaatgg attttgtgaa
     2281 gcattaaata cattgttatt aattgtcgct gcttttaact aggcctctcc ccttctatta
     2341 taaaagaaga tatatatcat ttcttaatta cctactacct taaaccaaaa tttaagtact
     2401 acctaattta agtattactt aaacaatcta aaaaatagtt atcccaattg ttatgctttt
     2461 ataatacgat tatttgtaac aaaatgaata ttatataatt aaacactacc atatttaggt
     2521 agtgtttaat tattagcaaa acaaaaaata tttattctat tctcttaact tcaactaagt
     2581 tatgctgcac acctttatat aaatcatatg caactcatta gctagacaaa ctacaagcat
     2641 gtagttttat ctgttaatta tgtactacct aaataaagta gtttataatt agcaagtata
     2701 tatatttaaa aatttaagat taattttttt atgacttgta caaatttatc tgatattctc
     2761 ttattaagaa ctacctaatt tgggtagttc ttatttttta cattttaagt agtatttaat
     2821 tgcaatttgg tagtattttt ttattatctt attcttagaa cggtagtact taatataaat
     2881 ataggtagta tctaatttaa gtattaatgg tagtatctaa attaacaggt agtatgtaac
     2941 tactttaaag taattttttt cagattaaca cagtagtatc ttattaaata agttaatttt
     3001 aatggtagta cataattatt atatatatct gacattggta gtacttattg tattttttag
     3061 tagtatataa tctattgttt tctagcctaa agccttgtct tataagaata atataatata
     3121 tactactgaa ataaggtagt atatattact agtaatggta gtatgtttta tatgcataag
     3181 gtagttctta atttattttt tgttttgaca ctggtagtac ttaattatct ttaattttaa
     3241 aactaaattt tattcatacg tttttctggt agtatttaat tataaataat atttatttaa
     3301 agtagttctt aatgacttta aaggtagtac ataattcatt ttatttctga ttttaggtag
     3361 tatttaatta aaaaacagtt ttaatgcgtt tgcatttttt atttatcttt gctattttcg
     3421 aaaagagtag gtaattttac ataaataaaa caacgtgaaa gtagaggatc tacaatgact
     3481 gattttaaat tttttaaggc ggatagagtt tacaacgaat tattttatca atttccaaaa
     3541 gtctttattg tttctgacga atacaaaaaa atgaaagatt caactaagat tgcctatatg
     3601 cttttaaaag caagattaga gatcgcaatc agcaaacggc aaatcgatga agaaggtaat
     3661 gtttatttta cttatacgac aaatgaacta tgtagagtat taaactgcca aaaacaaaaa
     3721 gcgatagcaa tcaaaaaaga gttggaatcc tttggtttat tattacaaaa gcagatggga
     3781 tttaacaaac agttagggaa aaataatcct aatagactat atctagcaga attaaaagtc
     3841 tcagaaaatg atatctactt actcgaaaaa tttgatagag agaataggga aaacgttgat
     3901 aaatcagagg gtatgaaaat catacccacc ctcgacgaaa aatcagacgc tgaatccctt
     3961 ggggctcaag agggtatgaa aatcataccg tgccaaaacg ttgataaatc agagggtatg
     4021 aaaatcatac cagaacttaa taataatata ttagacacta atagacacaa tatagacact
     4081 gaaaaagacc gcctacaaga tcaattgttg ttagacaatt ttgagacaat tatgacaaac
     4141 gacagcattg ctacgtttgt ccctgaacga gtattaaatt tgataaaaac attttcttca
     4201 agttacagtg aagctcaaaa aaccgtccag actattcata atgcaaagaa aaaagctgaa
     4261 atagaaagtg gtatttcgat agtttttgaa gaactcgata gttattatgt caatgcagaa
     4321 caagaattat acacgacact gttaaaagcc tatcaaaaat taaaaaccga aaaagtcgaa
     4381 aatatccaga acctgatttt tgtctatgta aaaaattggt ttatcgaaaa accaatagct
     4441 gctaaagtat caagtgaaaa acgtttgaat tatgaaagct ccccaagcac tattacgaaa
     4501 gactggttag agtgaaactt tactgacaaa gtgattgttg cttcatggag taacattaaa
     4561 attaaaaatt ttttattatt ctaattatat tacgaatatt acaaaataat agctatttta
     4621 atagaattta taagttaaaa tattatgata aaaatcacaa acgtagcctt ttacgtcata
     4681 agtgttaaaa gaaataatta atttcaaata tagaaaaggg aacgtatgtt tgcatactcc
     4741 ttttttttga gttataataa tacgcgaaga gagataattt cattatttaa acaagctaaa
     4801 aatcttgata ttacttcttt tcaggcaata cgaatattat tcgcttacat tttttgtata
     4861 aatttaattg tctgctatgc ttgtgatgaa ttgattttcg ttctgcactt ttggatgata
     4921 aatttggaca attgaaaata caaaaaaaag aaaggcggta tgaactcgga aggctggtaa
     4981 ccttcgtcag cgttcataca cggcattaag atttggccct taatgcgtta acctttcagt
     5041 gttacggtat tacccgcaa
//
LOCUS       DACEDM010000117         4604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEDM010000117 DACEDM010000000
VERSION     DACEDM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.124"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IWO50_002721"
                     /pseudo
     CDS             106..357
                     /locus_tag="IWO50_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IWO50_002722"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IWO50_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0359786.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IWO50_002723"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IWO50_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IWO50_002724"
     CDS             1786..1869
                     /locus_tag="IWO50_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0359787.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWO50_002725"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWO50_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0359788.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IWO50_002726"
     CDS             complement(2994..3857)
                     /locus_tag="IWO50_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ0359789.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IWO50_002727"
     CDS             complement(3859..4131)
                     /locus_tag="IWO50_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0359790.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IWO50_002728"
     CDS             complement(4148..4363)
                     /locus_tag="IWO50_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ0359791.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4604)
                     /locus_tag="IWO50_002729"
     CDS             complement(4455..>4604)
                     /locus_tag="IWO50_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAQ0359792.1"
                     /translation="QNQRSNKVSTWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQL
                     ENEKE"
BASE COUNT         1412 a          777 c          850 g         1565 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgctggt tctg
//
LOCUS       DACEDM010000118         4521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEDM010000118 DACEDM010000000
VERSION     DACEDM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.125"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            534..1445
                     /locus_tag="IWO50_002730"
     CDS             534..1445
                     /locus_tag="IWO50_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359793.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1932..2198
                     /locus_tag="IWO50_002731"
     CDS             1932..2198
                     /locus_tag="IWO50_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359794.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2270..2611
                     /locus_tag="IWO50_002732"
     CDS             2270..2611
                     /locus_tag="IWO50_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359795.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2666..3085
                     /gene="ssb"
                     /locus_tag="IWO50_002733"
     CDS             2666..3085
                     /gene="ssb"
                     /locus_tag="IWO50_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0359796.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3162..3458
                     /locus_tag="IWO50_002734"
     CDS             3162..3458
                     /locus_tag="IWO50_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359797.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3473..3904
                     /locus_tag="IWO50_002735"
     CDS             3473..3904
                     /locus_tag="IWO50_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359798.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4086..4391
                     /locus_tag="IWO50_002736"
     CDS             4086..4391
                     /locus_tag="IWO50_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359799.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1661 a          715 c          952 g         1193 t
ORIGIN      
        1 ccgacttcca ggtttttgac cattttctct caacgtggaa aaaccgccac ttatgagaga
       61 aaatggtcaa aaacctaagc gggaagtctc cgaataacat accaagcgcc gacagccaac
      121 agataaagaa tacactacta aaaatagtag tagcataaaa tttaaaaggg aaaaaatcaa
      181 cccctacccc agtcttgggg tctcaaaact aactcccccc taaccccctc tttcagcctc
      241 ttttgaaaga gggggagaat caaccccacg gccaacgccc cagtgttctt ggggctgtcc
      301 agaacagaaa cttgcgagag agtgggggta aaacaagtgt agaggaaact tgaagacttg
      361 ttcgaacaga aacttgcgag agagttggag taaaacaagt gtagaggaaa cttgaagact
      421 tgttcgaaca caaacttaca agagagggga gtaaagcagt tgcagaggaa tcaggaagat
      481 ttgttcgaac agaaacttgc aagaaagcat tattaaattg aaggtgatga aatatgaagt
      541 atttattgca tggctatttt attgattatt ctactcgcaa tagtagtact ttagcgattc
      601 tatgtttgct tcatgaattg agagtggtta ctagaaaaca gctatacaaa ctattgaatt
      661 ttgacagatc aattagtaaa caaggagtaa cgaatgcttt agtgagatta tatgaaaaac
      721 agttaatcga tcgaaaaaaa tatggaacag aaatgtatta ttatttgacg aaagagggac
      781 atcaaagcat aggtgggtat tatacgttgc cgaaagttcc tgaatataat ttgaatcacc
      841 atttacaaat taacaattat ttgattaaag gaatggcatt ggcaaaagac cacccacatt
      901 taaaacggat tatttcagaa agacgtaccg tctatgaaac gaaggattta aatcctaaga
      961 caaaaggaag aaaatttttt gtgccagatt ttatttttcg ttttcgagac aaagaaaatc
     1021 atgagatcaa ttggtccttt gaaattgagc taacgttgaa aacacgtcgt cgctaccgtg
     1081 aagcgatttt tccgaaatat attcgtgagt tgcgaacacg tcctgaatca catttaattt
     1141 atgtgacacc tagtggcgca attcaagaag aacttcatgc gtttaagcgt atttttttca
     1201 agcgcgaaac tgaaaaagat gcgagtcaac aagaattgtt tgagcggttg catatttttt
     1261 cagctttaga gtttgaagag ggattaaaag cattattaga gaatgatcca gcagtcaatt
     1321 ggcggacagt agaacgagaa aaaatttatc cgaatggcgc tgttcaaagt cgtttgaatt
     1381 tatttgaaga atcaactgaa aatgaggaaa aaccaaaagt gaagtttacg cctgaagtat
     1441 tgtaaaagag ggtgttcgct tcgctctttt tttgtgccag tttgctggca ccgttcagac
     1501 gcaagcgtct gaaccggctc gtttttagga aggactaact tgtttgattt atccgttgtc
     1561 aaagaagtgg acgttcgaga gtccctaatc gtatagcagt cattcggcga gtataaccta
     1621 aaacataacc tgccaattgc caagggtaaa atgcacgctg cgcgcccttg gcaatcgtca
     1681 gaacatgttt tcgagccggt tatgccgaac gaccgctata cgaaaagggc ctctaaacag
     1741 aagtccactt tgatccttga caactgaaac ccaaaatcaa acaagcaggc cttcgtctaa
     1801 aaacgagccg aatgaagacc ttgggggttg cacccccaaa cccccgtgcc cccgacacac
     1861 taccgaaaag gtgggggtgg gtggcgaaaa aatcaaataa aacattctat tcaagaaagg
     1921 aagaaacaac catgacaact aaaaacgagc aactgaaaga acaggcaaga aaagagttgc
     1981 aagaaagagg tttgattatt gaggggagct ttgaaggaga ttttgaaacc tatattggtt
     2041 gttatgcaag accgataaac aaacctacag ccttagaccc aatgaacgaa caagaagtaa
     2101 aagaacaaga aaaacatgca gtgaatggtt ttccgcaaga tttcactgaa tggtatgagt
     2161 gggaaattaa caacgggaag cttgaaaact tcttataaaa gtcagatgac gtgcaaagag
     2221 aatgattctc tttgtacggt aaaaatataa agaaagaagg aaaaagaaaa tggcaaaaaa
     2281 cacaccttat caagtaggag acatttttta tagtagttgg ggctacgaac aaacgaacgt
     2341 gactttttgg caaattgtga agttaacaga aaaaacagca tggtttcagc ctattcaaaa
     2401 gaaaacggtc gagcattatt cgtccatgag tggaaaagtt gttccaatga aagacaagtt
     2461 tatagagtat cctttagcaa aaactaaaga cagtattgta aaaaaacgaa ttgatccaga
     2521 aacctatcct gatatgttat gtggttatcg tagttgggac acgtttcata gatataaaaa
     2581 tcaagctgtt tatgaaagta gttattatta aggggatagt ttcccctttc aaaaataata
     2641 aatagaagaa tggagagaaa aaatcatgat gaattcagtg aatttagtag gacggttaac
     2701 aaaagaagtg gatttaaaat acacaccaaa aggcaatgcg acaggcacat ttattttagc
     2761 tgttaatcga aattatacga atgcaaacgg cgagcgtgaa gcagattata tccgctgtgt
     2821 catttggcgg aaagctgcgg aaactttagc aaaatatact ggaaaaggaa gtttgattgg
     2881 cattaatgga cgtcttcaaa cgaggagcta tcagaatcag caaggacaga ccatgtatgt
     2941 tacggaagtt ctagtgacag atttctattt gttagagagt aaagaaatca atgagcaacg
     3001 gtcaaaatca atggcgaata atcaaaataa ttcttcaatt ccaggaaatg agattcaaat
     3061 ttcagatagt tatttgccct tttaagtgaa aaataatttt gttctatacc tgatttcttc
     3121 aggtatagaa caaaaaataa atagagatga ggcgattttt tatgaaaatc attgacagtt
     3181 acgatcaacg aaccatgcaa tatttgggct cgtttgatta tacagatgaa aatattatta
     3241 attatgttgc aagtttacct ccttatcaaa gcgttcgctt ggtagatttt gcaagtgatg
     3301 aattggtatt aacaacgatg ggcaactttt tagattatgt cggagacaaa aaatggttgg
     3361 aaaaaatttt gtctaaattg attgcgaaac aaacaggaga agaacccata aatgcgattc
     3421 aattgttcaa tcgatatgaa caagaaaaca atgattaaag aggagagaaa aaatgaaaag
     3481 attaaaacga agtattttta ttgtgctagc attgattgat tttgttggga caggttgtga
     3541 agcaattgac gttcaaaaag ctcccttatc ggaaataaaa caagcaataa caaaacaaga
     3601 tgaagaacaa ccgctaaata cacaagtaga aatagtgaat caaaataaac cgagtttttc
     3661 tgaagaagat ttttcgttag aaaatggaac caggcaattt ttttcagatt tggatcgttt
     3721 aaatcgggta ggtgtagcaa gtgcactttt gcataaaagc aggatgccaa cagaagaacg
     3781 agaccgatta tacattaagc caactggctg gaaacaaaag aaaatgaaga atggcgatta
     3841 tctttataat cgttgtcatt tgattggcta tcaacgtgcg acaagtttcc ccataagtat
     3901 gtaatacgaa acgggaggat taatcttaag attaatcaca actaacctat ttgacagttg
     3961 gaatgaaagc tatcatgtct gctgaatcaa ctggtctgat actcctacat gcaataaagt
     4021 ctactaggaa gttattgtat gaagctaggt aaaggcggca gaacaatttc taagtgtgga
     4081 atcatatgaa gttggatatg ccgggacgct ataaattact cagggtgaga ctgtttttaa
     4141 ttaaactgac gaacttgcga atgtacgggt ctataaccga cttatattcg aaagatataa
     4201 gattgcgaat gcaaagaaca ttatcgaaaa gtaagaatgt tggtcatgaa attcgagatg
     4261 gaagactagc ggtgtatctg tcaagagttg gtaaaacatg tttgattgat tcagatgaga
     4321 gtgggaatgc gacaaaacgt tttacagaag aaatagagca actagcagaa ctgaatatac
     4381 gatattttta aatactttag cggagaataa aaaaatcctc tacagaaaac tacggagggc
     4441 aacatgtgct attgtttcaa caatacattt ttaattttta ccataacaaa aaagtacttg
     4501 aaaaaaaaca gaaatatgtt a
//
LOCUS       DACEDM010000119         4465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEDM010000119 DACEDM010000000
VERSION     DACEDM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.126"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWO50_002737"
     CDS             574..1008
                     /locus_tag="IWO50_002737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359800.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2302..3225
                     /locus_tag="IWO50_002738"
     CDS             2302..3225
                     /locus_tag="IWO50_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0359801.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3241..3780
                     /locus_tag="IWO50_002739"
     CDS             3241..3780
                     /locus_tag="IWO50_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0359802.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          668 c          759 g         1374 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaattatata agaataggtt taagagtgct taaatcgaat
     1741 tttagagggc atttagacaa ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     1801 gttttaagaa caagataagg gatttttata ctaggactgt cttaaaatgt cttaaacgaa
     1861 cgctcagagc ttctcaaagg gttccttatg aacgggcttt tagcaaagga gccggaggcg
     1921 actgcgcagt ataatgtgag agcagagcga tcacaccttt ttagcgaacc gtagggagca
     1981 aggtttggaa ttggcagttt tgataaattc gcgaacctgt ttagagaaaa aaggagtgat
     2041 tttttaggcg ggaaaattag cctcccctct ttttatatct ctttttatat ctctttttat
     2101 atctctttta tgtcggctgt gtccttactc tcccaagcaa ttcagagttt tttcaagtag
     2161 gttttctacg cttttcaagt aggttttcta cgcttttcaa gtaggttttc tacgcttttc
     2221 aagtaggttt tccgacatta ttgagtattt ttataaagta tgttactttt atttcatact
     2281 ttataaaagt aggagaagct tatggcaaat gaaattgtaa aatatgaaaa tagattaaat
     2341 catattcctc tgagaaaatt taatagtcgt gaaatgaact tatttttttc aattgcttca
     2401 cgtgtacgtg aaaaaggtgt agatgagatt attttttcat ttcacgattt gaaaaaacta
     2461 agcggttata cagaacgtgg ggaacaattt ataaaagatc ttagtagcac gtatgacaaa
     2521 ttattaagta ttaatgcttg gaatgatgat ggaaatactt taactaaatt tgttgctttc
     2581 acaaaatata gcattatacg agataaacaa cttgttataa tttcagttaa tccagaattt
     2641 aaaggacttt ttaatcaatt aaagaactgg acaagattta atctagagca atttactaat
     2701 ttggatagca catattcaaa aactatgttt cgattattaa aacaatatcg aactcaaggc
     2761 tggactgaat tttccaaaga aaaatttttt gatttactag atattcctaa aagttattgg
     2821 aatagtccgt caaatattga caaatttatc ttaaggccaa ttaaagaaga actcactccc
     2881 ttatttaaag gattaactat tcgtaaaaaa tatggtaaag gtcgaggtaa acctgttatc
     2941 ggttatcgtt ttacttggaa agctgaaatt aaccatgctg acgatttttc caaagggaaa
     3001 caagaagatt tacgtataaa attatttaat attgatcaca atggagaatt aacacgagaa
     3061 gaaaaatggc gcgctaaaga tcgaatacta aatcttccct taggcactca tgaagctgat
     3121 tttaaacaac aacaacaaac tgaagaaaaa caggcagaaa aacgagaact ttcaaacaaa
     3181 ctcaaacaag atttactagg aaatttacaa aacttattcg attaagctta aggagtattt
     3241 atggcagata aaacaatcaa agaacttgcg gaagaattag gagttagtaa aacagcaatt
     3301 aacaaaaaag tttctgacaa aaatcgaaaa ctttggtttg caaaaaacgg aaacaagttt
     3361 gtgattaacg aaaccggtca aaaagctata aaatcaatgt ttttagtcga taacgcaaac
     3421 ttaaatcgaa aactagttgg cgaaaaatca aaaaccaaaa accataaaaa caccaaccaa
     3481 gtttccgaat taaaactcct ttctttccta gaaaaacagt taaatcgaaa agatatacag
     3541 ctacaagaaa aagataatca aataaaacaa atgcaaaaat tactcgacca gcaacaacaa
     3601 ctcactctac aagcgaacca acaaattgag aacctacaag aacagctcca tttaacttat
     3661 acagaagaaa ctccaacaac cgaatcaact gccttttctg aaaaaaaagc agacaataca
     3721 caaccactta ttgaaaaaaa gtggtggcaa atttggaaat ctaatagaac caatctctaa
     3781 aattttctag aagttagttc ggatagtaga ggaaaaagtt gtactacctg atggggagat
     3841 cactcaaatt tttatgagaa agaaaaaata gtttacaact gtaaacaatc aatgtttata
     3901 gttgtaaact atcaaactat atggtgactt attgcttaaa ataggttgaa atctcactca
     3961 atttttggtg gaaaaacaaa ataaattcta atcgattttg ttgaaaaagt cataaaaaaa
     4021 caaatgtgat tttaacaaaa tttcaaaaaa atcgtttgaa atttcgttta ttttttttga
     4081 gaattttgat catcgtaggg tcattgtaga atcattatga gatcatcgtg ggatcatcga
     4141 ataaagtatg ggaaaggctt gcggtgcttg gtattaaggg cattctccgc ctcccaggtt
     4201 cttgaccatt ttctcttata agtgggattt ttaccacgtt gagagaaaat ggtcaagaac
     4261 ctgggaggcg gagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DACEDM010000120         4444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEDM010000120 DACEDM010000000
VERSION     DACEDM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.127"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            153..1859
                     /locus_tag="IWO50_002740"
     CDS             153..1859
                     /locus_tag="IWO50_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0359803.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="IWO50_002741"
     CDS             complement(2017..2919)
                     /locus_tag="IWO50_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0359804.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3620)
                     /locus_tag="IWO50_002742"
     CDS             complement(2916..3620)
                     /locus_tag="IWO50_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0359805.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="IWO50_002743"
     CDS             complement(3651..4292)
                     /locus_tag="IWO50_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0359806.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1376 a          946 c          768 g         1354 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gatt
//
LOCUS       DACEDM010000121         4388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEDM010000121 DACEDM010000000
VERSION     DACEDM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.128"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="IWO50_002744"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="IWO50_002744"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0359807.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="IWO50_002745"
     CDS             complement(1061..1981)
                     /locus_tag="IWO50_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0359808.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="IWO50_002746"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="IWO50_002746"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0359809.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3587..4120
                     /locus_tag="IWO50_002747"
     CDS             3587..4120
                     /locus_tag="IWO50_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0359810.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1287 a          866 c          778 g         1457 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaactggctt ttgaacactg tttatcgaga gattgtgaaa aagctgtcct gcatcaagta
     3001 ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg
     3061 cagatacaag cttttgaaca ccgtttatta agaggttgtg aaaaagctgc actgcatcaa
     3121 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt ggacttatta
     3181 ccgcagatgc aagcttttga acaccgttta ttgagaagtt gtgaaatcag cgtcctgacc
     3241 tgagtattag agaaacaaat ggcaaaatag ttcctcatat tttgctattt tttgagctaa
     3301 taccgagggt cagctgattg aacaccgttt agtggagaag ttgtgaaaaa gtcgcctact
     3361 ttcgagtcgt aagaaatttt tttgaaaatg gttcctcaca actttgcata aatttgactt
     3421 aataccgaga agtcatgatc cccatctaac tccaaatcac aaggaatgag ttcaaaaact
     3481 agcttttctc aacaaatatt acataaaaaa ttctatgttt tttgttaaat ttatttgctt
     3541 ttttcaaaag tatgttacag taatttccca aggaggtttt acagaaatga attttgttgc
     3601 catggatttt gaaactgcca atcatcagcc ttatagtgct tgttctcttg cgctagtcat
     3661 ggtaaaaaat agccagatcg ttgatgaatt ctatacattg atacagcctg agactccttt
     3721 tttctggcga aatgtccaaa ttcacggaat ccatcaagaa gacgtacgca atgcgccgaa
     3781 attcccagaa gtctggcaaa ctatccaacc atattttcaa aaaaatcgtt tagtcgttgc
     3841 acataatgct gcttttgata caaaggtctt agcgggatgc ttagattatt atcatcttga
     3901 acagcctaat tatctttctt tatgtacagt gaaaagtagc cgtcgcctgt ttcctgaatt
     3961 tccaaatcat cgattgaaca ctgtgtgcga aaatttgaac atccaattag atcatcacca
     4021 tgatgcattg gaagatagtc gtgcatgtgc ggaaatcctg ctttatcaag aaaaaaaatt
     4081 cgggacagat ccattgaaaa aacttgtgac tataaagtaa aagaatactt atgaaacaca
     4141 caaagaggtc gtgagcctgc cgtttcactc cgagtcacaa ggagcaattt caaaaaatag
     4201 cttctcatgt ttattaaata ttgtgactgg tgaccgagga gctggcagga gaacaccgtt
     4261 tattggagag gttgtgaaaa agctgttctg catcaagtaa taagaacagc caaacaaaaa
     4321 tagcttctca tatttttcat ttggttggac ttattaccgc agatgcaagc ttttggacac
     4381 cgtttacc
//
LOCUS       DACEDM010000122         4377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEDM010000122 DACEDM010000000
VERSION     DACEDM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.129"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(83..1051)
                     /locus_tag="IWO50_002748"
     CDS             complement(83..1051)
                     /locus_tag="IWO50_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0359811.1"
                     /translation="MSKNGIIVNEWEKLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1501..2907)
                     /locus_tag="IWO50_002749"
     CDS             complement(1501..2907)
                     /locus_tag="IWO50_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0359812.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDIGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            complement(3129..3491)
                     /locus_tag="IWO50_002750"
     CDS             complement(3129..3491)
                     /locus_tag="IWO50_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359813.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3495..3803)
                     /locus_tag="IWO50_002751"
     CDS             complement(3495..3803)
                     /locus_tag="IWO50_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359814.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3812..4231)
                     /locus_tag="IWO50_002752"
     CDS             complement(3812..4231)
                     /locus_tag="IWO50_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359815.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4224..4364)
                     /locus_tag="IWO50_002753"
     CDS             complement(4224..4364)
                     /locus_tag="IWO50_002753"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359816.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1235 a          989 c          618 g         1535 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aatttttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactttatt tttttgctct
     1261 tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct atgcaaatat
     1321 ccaaaaacat aaaaccgcac cctttgaaaa gggcgccccc aaacttcgct aacgctaggt
     1381 tggccaaaaa atggactggc cattttttag caaatgtcag cttaaaaggc tagtggttta
     1441 gccgtcaact ccttatcctc ggcacaaacc gcctccggta ccgttgactg cacatcgtaa
     1501 ctaccactcg atctcatctt cagaacgctc taatttatgc tgtgagacgt tttgacgata
     1561 aaataaggtt ttctcctgct ctaatacttc taattcctca gaaagcccta tatccttttc
     1621 tgcaagacca aaacggatag acaaacgagt gatcgatttt tgaaatgcgt tttttaatcg
     1681 ctctaaacgc tggcgtaaag agcgattttc ttctttcagt tttttatttt ccacttgtaa
     1741 atctgctaat tgttttctac cagacaaatg acgctcttct tccaatgtag ctacatactc
     1801 atttaattct gtattttgct caagcatctg taaatattgc tcactcatgt tatcagcatt
     1861 tctcttggca tttgttgctc tcgtagctcg ttgatacaaa tcaacaaaat cattcccaga
     1921 taaatgatac gaaccatcag gaagcctctt gaaactttct aaaggaacca tatctttttg
     1981 gaaaatgacc gccatctctt tttttacatt cagcatattt ttcttctctt cttcaatctt
     2041 cgctctctca gaagccatca gagccttatc acgctcgttt tcttctcttt gcctataaat
     2101 atacctcaac tcatcagaaa cgctctcacg ctcttttaca gccgtttggc gcaattcaga
     2161 aatttcactc atgatttctt tgtattcgtg aatatcttta attttgttgg taccgacaat
     2221 atcccttttc caaccgtaat cattcattaa tcgttctata gagtcaactt cttgatttct
     2281 aaaattattg aaaagcactc gaccattatc tttatcagcg tgtatccctt gttgaaccag
     2341 tgctttatta aagctcggct gcttttgaac accccgttta taaccttcag caaccggcac
     2401 aatattcaag tgcaggtgcg gacttatttc gtcattatga ataacagcat tataaacagc
     2461 caaattaggg tttctctctt gaaactcctt gtaatactga accaaaattt tattggcaat
     2521 cactcgattt tgttcagctt gttgtttcga tatgccagta gaagaaccat ctgctcgatt
     2581 tgtctggaaa tcatctaaaa tgcctatttg cacaataaat tctcgttgag gttccaactt
     2641 tttatccttg cgcactttcg ctaaatagtc ctcgattttt ctatcggctc ttttttgttt
     2701 cgcattatat ttttctacca cttcgccaaa caattcctca tatttatctg ctagattcgt
     2761 ctgctctaaa taaatatttt catccgattt cgtgaagtca atatgcttat gccaatcatt
     2821 ggttttctgg ctatcagtta actcacgatt attatgagaa atacttgtat tcttcgttcc
     2881 ttttttaaat gaaatactaa accccaagtt aaaaccctcc ttcgcgctgt cgctaataaa
     2941 aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta attgtgacat
     3001 ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga caacgaaaaa
     3061 aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc ttcacgcaaa
     3121 ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact ctcactcatc
     3181 tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg ataatctcgt
     3241 tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt ttcgtgttgc
     3301 tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc aaacccaaaa
     3361 cgcttgcgaa gagccttgcc cattgcagca ttttctttct caatcaagcg ttttttctgt
     3421 tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat atctgctaaa
     3481 gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat ttggtttaac
     3541 tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc agttgtttgc
     3601 gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctag atcagcattg
     3661 ataaccgagt tgagaacatc catcattcgt tcaagcattt cactataatt atcttcatcc
     3721 ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc gactgtttct
     3781 tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac cattttcgat
     3841 tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga agcaaagtca
     3901 atttttgttg gtcacgaatc atttccgtta attcagaaat atgcctatct ttttccgcta
     3961 attgttcacg caaaaattca ttttcttttt ctaacgggaa atcattgcct gcatcattga
     4021 ccggtaaatc accagtcaat tgaccacctt ttgaccgata aattgaccgc acaacaccaa
     4081 tcaattcctt gtctaacatg ataaccccat tatctttata ccgagcaagg tcataacctg
     4141 cattgaccag ttgattgcgc actgtatttt tagatatacc caactcatca gcaaggtttt
     4201 ttatggtcat tttttctggc aaatcagtca ttatcttctc cgttactttc agctacccca
     4261 taaacatatt taacagcatg cttagggata atgattgggg aatcaatttt attatttttc
     4321 aaaatcaact catactgcgt tacatcaagt aacttaccgg acattttttt gctatcc
//
LOCUS       DACEDM010000123         4193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEDM010000123 DACEDM010000000
VERSION     DACEDM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.131"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            119..625
                     /locus_tag="IWO50_002754"
     CDS             119..625
                     /locus_tag="IWO50_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0359817.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="IWO50_002755"
     CDS             642..1541
                     /locus_tag="IWO50_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0359818.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="IWO50_002756"
     CDS             1628..2512
                     /locus_tag="IWO50_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0359819.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="IWO50_002757"
     tRNA            complement(2704..2775)
                     /locus_tag="IWO50_002757"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="IWO50_002758"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="IWO50_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0359820.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
BASE COUNT         1520 a          765 c          844 g         1064 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gta
//
LOCUS       DACEDM010000124         4157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEDM010000124 DACEDM010000000
VERSION     DACEDM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.132"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IWO50_002759"
     CDS             179..775
                     /locus_tag="IWO50_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0359821.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IWO50_002760"
     CDS             899..2698
                     /locus_tag="IWO50_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0359822.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IWO50_002761"
     CDS             complement(2976..3188)
                     /locus_tag="IWO50_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359823.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4052..>4157)
                     /locus_tag="IWO50_002762"
     CDS             complement(4052..>4157)
                     /locus_tag="IWO50_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0359824.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          706 c          729 g         1494 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DACEDM010000125         4135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEDM010000125 DACEDM010000000
VERSION     DACEDM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.133"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            572..1384
                     /locus_tag="IWO50_002763"
     CDS             572..1384
                     /locus_tag="IWO50_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359825.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            3019..3573
                     /locus_tag="IWO50_002764"
     CDS             3019..3573
                     /locus_tag="IWO50_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0359826.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            3827..>4135
                     /locus_tag="IWO50_002765"
     CDS             3827..>4135
                     /locus_tag="IWO50_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359827.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT         1531 a          509 c          656 g         1439 t
ORIGIN      
        1 aaaaaaagca gttatttgtt aaaatcattg atattattaa taataagtat tttttgaaag
       61 gggaaatata aaaggttttt gaaaataaat aaaaaatagt attacttgtt ctattgctag
      121 ttattgctag tctttattct tttttgtttt ttagggatgt acaaaagatc tcaaccacag
      181 gagacattgc ttttcacttt tctagaataa aatgcttgtc ctcagtattt tctggtccaa
      241 taaattttac cacatttaat agctatggcg gaggcgtgaa tttttttatc catatctaac
      301 atctttccag ccgtaatatt ttactggaca acaaacaatt tgattctaag ttatatactt
      361 actatacgtt gggatactga atatctgtac aattctagtg agctatcaat atggacaaaa
      421 gaatagatgc agccttctta ttcagctgtt tgtatagctt ttatggattg atatatatta
      481 gcgtactgca atttcagaat caattgcgtt gacgattgta ccagttgtct tgtattacac
      541 atatactctt ctttatgaaa gaaaaaactg cgtgattctt ttagctgtca gtatgagttt
      601 gctcatctat acccatattc tctccacctt tatgagtgtg attgttattg gcttcttgtt
      661 tatcggctct cttttctcta agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa
      721 tatgataaaa gctgttggtt ggaccattct tttaacagct tacgtttggt atccgatgtt
      781 acaacaagtc ctacatcaaa aaattaatcg tccttttcgc acaaaacttc aagaaagggc
      841 attaaacatt tctgatagtt taatcggtgc agtaaataat gatttagtta catttacaat
      901 gggaacaatt ggtgtggttt catttattct tccacttttc tttaaaaaaa aatttgccaa
      961 taaagaaaag attattaatt tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt
     1021 tccatgggaa tttttacaaa aaacacctat tcaaatcatt cagtttcctt ggagaatctt
     1081 gggttttcaa gtgctttttg gttcattgat cttaattata gtgtttttaa agtggaaaac
     1141 aagcaacaaa aaaagtatgt gtagccttgt aggaatagtc cttcttattt ttacagtgac
     1201 tgtagcaaca gaagcgaatt attcgcagaa aattcaatcg tataaaggtc gtttaattat
     1261 gaccaaaaaa gatgttacac tttatacaac ctcgagaact ggaggtcttt atgactatgc
     1321 accattagat gcattgaaat ataaagatca tttaaagaaa catgaagtaa aagtctatga
     1381 ttgatgggag aatgcatctc ataaagcaca taattctaaa ataatctata cagtaggaga
     1441 tgcgaatgag caaaatatca cgttacctgt atttaattac tgggaacagt tatgaaagta
     1501 aatgggaaaa agagcactat agaaaatgac aatggtttag tgtctattaa aggtacggga
     1561 aaaagaacta tagttgaggt ttcatctacg tatccaacta cgatgattct atctttatta
     1621 attagttcaa taacttttat attcttattt acacgttatt taataagatg ctgcggttaa
     1681 atgaaaatag attagataga tattataaat atgttgattc tattttcata gcaatggaga
     1741 aaatacatta gcaatatata aaagaaggta tcaagagtca cggtgaattt attccgaaaa
     1801 gtgaaaagaa caaatataaa atttaataaa aatatagaac taaataggaa aacgtagacg
     1861 taattttcta cgttttcctt ttcatatata ttaaatatta cggaaattta tataaactaa
     1921 tttagtgttt tgtttgtaat tcctaatttt gctccacttt tagataattt ttaggtctat
     1981 ttaactcatc agacattcta tgctcatttt ctccaaattt gtttcaactc tttcgtactt
     2041 aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac tagtatgata
     2101 tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt cctaaaacct
     2161 ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa catgaaatta
     2221 tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa catttattga
     2281 ttgcaaaatt atgtgacaaa aagaactagt agaagaagga ataatggctt gataataaag
     2341 tactacctat taaaggtagt actttattta atttagtata aattaacata ctactatatt
     2401 ttaagtttca tattatataa attaataata tactaccatt ttttggtagt ataatttaaa
     2461 ggtaataagt tattctttta ttactatgtt tgggttttat agcaaggaaa gtttaataag
     2521 agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct ttagtgatat
     2581 aacgaaatat ctacacagac aaaattatga tcttgcgtaa agttataaaa tattaataaa
     2641 aatattttat taatgaaaag cttggtagca cggttattca tatgctaatt tatttcactt
     2701 tatagtaagg ggagttctta gtatattgtc caaatttaaa tttcatataa tttaattatg
     2761 taaattagct attctaagaa cttctacgga taactgcccg tctaaaattt aattaagagc
     2821 ttagaatctt catataagta aggttaattt ttttgtacaa aatagtcgga atattctgag
     2881 ttactgaaat gatataaaat cataaactaa accccttgat tttcttcact tttaacagag
     2941 tatatatata agttcatgat tatggtaaaa tatatatata atcatgaact cagaatattt
     3001 ttaagaaggg aaacaatagt gagctataat gtacaaccgt taaggacaaa tgaagagata
     3061 caagattttc agttttggct aagaagaaca agtaatccag aaagagatag ttttttattt
     3121 ttgtttggaa tcaataatgg gttacgtatg tcagatatta taggattaaa agttggagac
     3181 atacgaggta aaagtaagcc tattattgtt gaacataaaa caggtaagcg aaaaccgatt
     3241 tttatagata atttgagaga agagatttta ttatatactg aaggtaaaga agaaaatgat
     3301 tggttatttc caagtcggca acaaggtcga catataacaa gggatcgtgt ctatcagata
     3361 tatgcagata tagcagaaaa attaggaaga gatgatatag gaacacatac attaagaaaa
     3421 acttttgggt atcactacta caagaaaact agagacattg ctaccttaat gtttattttt
     3481 aatcatagct cgcaagcaat tacaaaaagg tacattggta taactgaaga tgaaataggg
     3541 gcatcgttac gtggttttaa attgggaggc taattgagat attaggatat attttttgtg
     3601 tataatgaaa acttagaagg tgagattaat gaagataact gaattttcgg aagtatttaa
     3661 aacgtttagt aacatttaca atagaatacc agagatagaa caaaatgttg tagttgataa
     3721 tgattattat atagagaatg attttaataa accaagaaaa tatgacaacc tatacaattt
     3781 tatacacaat agaacaacaa taaataacaa cagtaacact cgagttttga ttaattcagc
     3841 atattttcat attaaatatt taaagaaata tgagtatgaa gaagttgtat tagattatgg
     3901 gctaaataga cagtcatttt taatttatgc aataaataac ggaaatttgc caagcaataa
     3961 aaagaacata attattgaaa catatgccta tctggagggg ggatctaaaa aactaattaa
     4021 gtcatgtaat atagagtatc aattatcagt aggagaaatt aaatgtgttt tcgaagaaga
     4081 tttagaactt gacttaaaaa aaatttttgc cagcattcat gattatatag gtttg
//
LOCUS       DACEDM010000126         4060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEDM010000126 DACEDM010000000
VERSION     DACEDM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.135"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            137..559
                     /locus_tag="IWO50_002766"
     CDS             137..559
                     /locus_tag="IWO50_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359828.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            569..772
                     /locus_tag="IWO50_002767"
     CDS             569..772
                     /locus_tag="IWO50_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359829.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            983..1567
                     /locus_tag="IWO50_002768"
     CDS             983..1567
                     /locus_tag="IWO50_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0359830.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1938..3263
                     /locus_tag="IWO50_002769"
     CDS             1938..3263
                     /locus_tag="IWO50_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0359831.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            3256..3606
                     /locus_tag="IWO50_002770"
     CDS             3256..3606
                     /locus_tag="IWO50_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359832.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3603..3782
                     /locus_tag="IWO50_002771"
     CDS             3603..3782
                     /locus_tag="IWO50_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359833.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
BASE COUNT         1433 a          597 c          790 g         1240 t
ORIGIN      
        1 aaatgtcaat caagaaaatt taaacgatta tgacattaaa aatatatttt atgaattttc
       61 tagtatataa cttgatttat ctgttaagat ttattaagaa aacgttttct tagttttgat
      121 ttcgaggtga tagacaatga aaaataagtt tattttatct ttaggtattt taactattgg
      181 aattagtgta actggaacta caattcctac atttgctgat agtacaaatg aaccaactac
      241 tgtagtagat aataaccaag aattaactcc tttagatagg gacggaatag ccattggtaa
      301 cccaactaac gacttaactg ttactgaaaa ttatttaata gtttctccat tacttgctcg
      361 agcagcttct aaaacgatac gagtgacagg aacgatgatt tttgataaag ttggttggcc
      421 ttctagacct cctcaatcac ggaattggaa agaaaaaaga aatggttatt ggtactatgg
      481 aaaattgtat ttacaaagtt ataaatcagc tggaaacaat tggattgcga cttattcagg
      541 aaccttaact agaaagtagg gattttttat gccagaattt gtgaacacac caaattttta
      601 tcaagatagc gatattctaa gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt
      661 tcagcgaact ttacaggaga cacaaaaaat tcaaaaatca atttctaatc tagaagattc
      721 tgaagaaaaa agaaaaaaaa gagaccaaaa aacatatcaa aaagaaaaat aatgatttta
      781 aaaaaagagt gtctagtcga cattcttttt ttctaattga aagtgttcat aaaatcaccc
      841 atttctggac acttcctttt tttgctttat ttttcaaaaa aacaattcat ttcatgttca
      901 taaacccata acaatatcta tgttcagtta aactatatgg ataaagttat gttacaataa
      961 aattaatgaa ggagtgattt ttttgaaaat aggatacgcc cgggtatcta ctggtttaca
     1021 aaatttggat ttacaagaag acagcctaaa taaatttggt tgtgaaaaaa tttttactga
     1081 tcatatgagt ggggcaaaaa gtaatcgacc aggattagaa atggccattg attttgttcg
     1141 ctctggcgat acacttgtcg tttggcgctt agatcgttta ggtagaaata tggaagattt
     1201 gatttcaatc gtcaatcgac tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa
     1261 tattacgatg gacaaatcaa gttctacagg acagctcatg tttcatttgt ttgcggcatt
     1321 tgcagaattt gagcgtaatt tgatcctgga acgatccgca gcaggacgag cagctgctcg
     1381 tgcaagagga cggtttggtg gtcgtcctga aaaattgtct gaacaagatt tggaattact
     1441 gaagacttta gtggatagcg gtacacctat taagactatt gcggaacggt ggaacgtctc
     1501 aagaaccacc atttatcgtt atttggcaaa aatggaggag aaaaatggaa agacaaattc
     1561 tcattagttt ttgtcattta atgctgaaaa tttttgccat ttatctagaa aagtgacaat
     1621 agtatgagaa ttttttgtaa gtgtctactg ataacttatt atcaaatgtt gtaaggattt
     1681 tattcatttg ttaatattaa agaacggaac tccaaaaaag tccgtactct aatttgcagt
     1741 ctctcactct tttagagtga gagtttttat tagattttca aattaattag taaagtgata
     1801 gaataaattg atcatacaaa tcataagaag aagcagggtg atagcatacc tatgcttctt
     1861 tttttatgta tttagctatt tcttctttac aacatcgaac atatgtttgt ataatgtgga
     1921 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttaa
     1981 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aagaaattta gatccattaa
     2041 caacagaact tgttgttatg agtcgatctg ataatactgg ttcaggattg atattagctt
     2101 cttctcctga agcaaaaaag cggtatggta ttacaaatgt gagtagacca cgtgacttac
     2161 cacaaccatt tcctaaaaca ctacacgttg ttccaccacg tatgaaccta tatatcaagc
     2221 gaaatatgca ggtaaataat attttcagaa gatatgtggc tgatgaagat ctactgattt
     2281 actcgatcga tgaatcgatt ttaaaagtga caaaatcact gaatcttttt acgactgaag
     2341 aaacacgaag tcagcgaagg aagaagctcg ctcaaatgat ccaagaacgt attaaagaag
     2401 agttaggttt gattgctacg gtaggcgtcg gcgataatcc ccttttggct aaattagcct
     2461 tagacaatga agcaaagcat aatgaaggat ttattgcgga atggacgtac gaaaacgttc
     2521 ctgagaaagt atggaatatt cccgaaatga cggacttttg gggaattggt tctcgaatga
     2581 aaaaaaggct caaccaaatg ggaattttga gcattagaga ccttgctaac tggaatcctt
     2641 atacgattaa gaatagatta ggtgtgattg gattacagct gtacttccat gctaacggaa
     2701 tcgatcgaac agatatagca atcccgccag aaccgacaaa ggaaaaatct tatggtaatt
     2761 cacaagtgtt accacgagat tatactagaa gaaatgaaat cgagctggtg gtaaaagaaa
     2821 tggcggaaca agtagccatt cgtatcaggc agcataattg taaaacagga tgtgttcatt
     2881 taaacattgg tacgtcgatc ttagaaacga gaccaggatt ttcacaccag atgaaaatac
     2941 ctatcacaga taatacaaag gaattgcaga attattgtct tttcttgttt gataagtatt
     3001 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacaccgatt
     3061 cgcttcagct ggatttattt agtgatccac aaaagcaaat taacgaagaa aatttagata
     3121 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcaa
     3181 tgttagaaag cgctagaagc attactagaa gcacacttgt tggtggtcat gcgggcggaa
     3241 atgggggaat taaaaatgat tgattacaat gatcgtggac gtttaaagtg ggcagggttt
     3301 tatttatctg atcatactga agaaattgat gccgacaatg aacaacgaac cagtcaaaat
     3361 ttagcaaaag aacaaatgac gactgaagag attgcggaag tattgaacgc tgcagtcata
     3421 aaaaatagat cagtatctat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     3481 gatattgttg gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaagacaaa
     3541 attcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gagtcgatta
     3601 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     3661 agatttgggg ttatggccaa cgtttgataa atgaggttgg tgttgagcgc ttctctttct
     3721 atgtagaagc tgcagcaggt tatcataatt ttaggtttta tattttcccg ttatatatat
     3781 aaggtatttt acggattcta tagagttatc tctatgaatc cttttttgtt tttacaaata
     3841 ggagtagata ctagaataat gcgtgtgtta aactattatt gcaaacgcaa taataaaaga
     3901 aagtaggctt acgcaccatg tctatcaata catttgttta ctcgcacccg atcaatgttt
     3961 acattatcaa gaatttagga atcactgtag aacaattttg tgagttatat gcctacccac
     4021 aaggaacggt tgcaagctgg ataacccgac aacgtagaat
//
LOCUS       DACEDM010000127         3956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEDM010000127 DACEDM010000000
VERSION     DACEDM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.136"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IWO50_002772"
     CDS             <1..156
                     /locus_tag="IWO50_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0359834.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IWO50_002773"
     CDS             175..1452
                     /locus_tag="IWO50_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0359835.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IWO50_002774"
     CDS             1462..2118
                     /locus_tag="IWO50_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0359836.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IWO50_002775"
     CDS             2118..3494
                     /locus_tag="IWO50_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0359837.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="IWO50_002776"
     CDS             3624..>3956
                     /locus_tag="IWO50_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0359838.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          424 c          693 g         1245 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagacagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACEDM010000128         3914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEDM010000128 DACEDM010000000
VERSION     DACEDM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.137"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..1530)
                     /locus_tag="IWO50_002777"
     CDS             complement(25..1530)
                     /locus_tag="IWO50_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0359839.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1527..2213)
                     /locus_tag="IWO50_002778"
     CDS             complement(1527..2213)
                     /locus_tag="IWO50_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0359840.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IWO50_002779"
     CDS             complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IWO50_002779"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0359841.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1082 a          837 c          676 g         1319 t
ORIGIN      
        1 aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag gaataaagat
       61 tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt tatagttttc
      121 aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc tagctcttgc
      181 tttatccact cgataaaaac gattgaagat cttattaagg tcttctttgg gtattccttc
      241 gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag agatatggac
      301 ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga ttaattgttc
      361 aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat ctaaagtgat
      421 cacatagtct ggatataata tcttgaaatt attataaact tgataggtga cttcttttcc
      481 tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata agtctagcat
      541 ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta atgattcttc
      601 taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc cttcaataat
      661 tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt gttgttcaat
      721 atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg ccaattcatc
      781 atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta tagtatccat
      841 tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga aacctaaaat
      901 actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata gcttatttcg
      961 aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct tttctcttgt
     1021 acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta gtgtaacgat
     1081 cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg ttttaaaaat
     1141 caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta gctcagctat
     1201 aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta cgacctcatc
     1261 tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca gttcttcatc
     1321 cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt ttctttcttt
     1381 tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg taaacacaac
     1441 gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc ctttcagttc
     1501 tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt tccgcgtaca
     1561 gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata acgaatatat
     1621 acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt tagtaaaacg
     1681 tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc gtattctctt
     1741 tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt ttcgatagtc
     1801 aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc aatatcgatt
     1861 cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg tttgacgatg
     1921 tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc tcttgcagtc
     1981 atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc taaaccgttc
     2041 aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa tgccgcatct
     2101 aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt caactctagc
     2161 tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct catttattat
     2221 tttatccttt ctattctact ggctttttaa aaactctatc attataccga taatatgctt
     2281 aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga agtctactca
     2341 taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta acagccgaaa
     2401 gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc gacacgctca
     2461 taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg cagacgttct
     2521 gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg tacgccagct
     2581 tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt gatttcaacg
     2641 aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc atccgtaatt
     2701 ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc gatttcacca
     2761 aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa tccttgggca
     2821 taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa ttcttttttg
     2881 tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg tacgcgttct
     2941 tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg aagtggcaca
     3001 cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc agctgcatca
     3061 aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt cagaatgtca
     3121 gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt aaagatatca
     3181 gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga ttcagcaatc
     3241 agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg tccggctcca
     3301 tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga aatttgctct
     3361 aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat tgaaggtcct
     3421 tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat gccagagttg
     3481 gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc gtcaatcaaa
     3541 atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt tgccggtcct
     3601 gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa ttcttcgatt
     3661 gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt tgttttagcc
     3721 cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa ggctagattt
     3781 ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg aactcctcct
     3841 acatacggga tttctcccta atctcttaca ttatagctta acacggctac attgcaaact
     3901 aaagaacaac atat
//
LOCUS       DACEDM010000129         3747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEDM010000129 DACEDM010000000
VERSION     DACEDM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.138"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(55..513)
                     /locus_tag="IWO50_002780"
     CDS             complement(55..513)
                     /locus_tag="IWO50_002780"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0359842.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(684..1829)
                     /locus_tag="IWO50_002781"
     CDS             complement(684..1829)
                     /locus_tag="IWO50_002781"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0359843.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1842..2759)
                     /locus_tag="IWO50_002782"
     CDS             complement(1842..2759)
                     /locus_tag="IWO50_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0359844.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3187..>3747
                     /gene="efp"
                     /locus_tag="IWO50_002783"
     CDS             3187..>3747
                     /gene="efp"
                     /locus_tag="IWO50_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0359845.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1149 a          722 c          722 g         1154 t
ORIGIN      
        1 aaatacgaag ataagatagc tatattgctt ctcatcttta cgttctttta gaaactatgc
       61 ttctacttca gaccattcat cgcgttttgc taaaaattca cgagccaaag cttgtgcacc
      121 ttctagtgta tggctggcag cccagccaca ttgtgtttca ttgcttgcag gtacttcagt
      181 agcttcaaga acatctttca ttgttgcttc aagaacatct aaaatgtctt cgtagctttc
      241 atgattcaat acagtcaagt aaaagcctgt ttggcatccc attggagacc agtctacgat
      301 gtaatctgcg tgattgcgga ttagttcggc agtcaagtgc tcaagtgaat gtagacttgg
      361 catgtccatg tgttctttat ttggttgttt aaagcggacg tcgtatttaa tgatgacatc
      421 gccatgcgca cccgtttttc gatcagctac acgtacataa ggtgctttga cttttgtatg
      481 gtcaagatta aaactttcga cattcattct catttgtaag actcctatct gtttgttcat
      541 tcgttttgat gcttttcact aaagaattat ttagtgaaat ttacaaaaac gtctttctac
      601 ttgattctaa cacttatcgt ttccaaggtg tagcaatctg aaatattttt tagatttttc
      661 tgctaagtcg tttattggga cttctagttc ttgattctgt caaatgcctg agctaaatcg
      721 tttatgagat cctgaccata ctcaattcct acagaaagac ggatcaagcc gtcctttatg
      781 ccagcctctt ctcgtttttc ttttggaata gacgcatgag tcatgacaga aggaatttcc
      841 actaaacttt ctacgccacc taaactttct gctaaagtaa atagttggag tgattcaaca
      901 aatggaatag cagattcttc attttttaaa gtaaaagaaa tcattccgct aaagccgctc
      961 atctgctgct tagcaactcc atggagttca tgatcgggta atcctggata gtagaccttt
     1021 tctactgctg gatgagaaaa tagaaaatca gctaccacaa aagcattttt ttgatgttcc
     1081 tccatacgaa cacttagtgt cttgattccg cgctgaagta accagctatc ttggggacca
     1141 agtacggctc cgatagcatt ttggtaaaat ccgatctgat cggctagatc tttatggtta
     1201 gaagtcacta aaccagctac tacatcgctg tgtccgccta gatatttggt accgctatgg
     1261 acaacgatat cagaaccgag atctaaaggt ctttgaaaat aaggagtagc aaatgtattg
     1321 tcagcaataa ctatcaatcc atgttgatga gccagagtgg cacttttttc taaatcagta
     1381 atctttagaa gcggattgct aggtgtttca agataaagtg cttttgtatt gggtttgatg
     1441 gattgttcta ttttatcaag attactcgta tctatgatcg tgtactcgag accattttta
     1501 gtaagaactt tgtcaaatag acggaatgtt cctccataga catcatctcc taataagata
     1561 tgatcgccag cttgaaaaag agaaaaaacg gcatgaatcc cagaaagacc agaagaaaaa
     1621 gcgaatcctc gaacaccgcc ttccaaatca gcaatcagtt cttccaatgc aaaacgagta
     1681 ggattaccag atcttgaata ttcatattgt ttaggctggc cgacgccatc ttgccgataa
     1741 gtggatgttt gataaatagg tacactgaca gctcctgttg tgggatcttc actgatgcct
     1801 ccgtggatca attttgtttg aatgtgcatg tttctttcct cctataagta aatgtttttt
     1861 gataaatagc ggtctgctgc gtctggaaaa atcgtaacga cacgatgacc aggtggtaaa
     1921 cgacgaattt ctcttaatgc tgctgcaaaa gccgcaccac tcgaacttcc aactaatagt
     1981 ccttgttctc gtgctaactg cctagtatag ttaaaccctt ctacatcaga gattgtctcg
     2041 atctgattga ttgaaagggg agaaagaaac ggcggaatga attctacacc gataccctca
     2101 atttcgtgag gggctggatc accaccattt aacacagagc cctctggttc tacaccgatt
     2161 attcgaatgt cgggatattt ttctttcaaa aaggtagaag ttccagcaaa ggttccacca
     2221 ctcccaattc ctgctacaaa actatggata ttttctttga tttcatgaaa gatttctggc
     2281 cctaacgtat gatagtaagc agcaggatta tctttatttt caaattgaag aggcagatag
     2341 ctgtttgata tctcctctgc aagtttttta cttttgctga tggcaccgat gattccttgc
     2401 tcactaggag aatggatgat ctttgctccc aatgctttca tcaattgttg tttttctaag
     2461 ctaaacttct ctggaacaac aaaaaccgtt cttaatttgt atgtgagtgc ggccagagcg
     2521 atcccaattc ctgtatttcc ggcagttggt tcgatgatcg tcgttttttc attgattttt
     2581 ccttgggcaa atccttcttt gatcaaataa ctgcccaatc tatctttgat acttcctcca
     2641 ggatttaaat attctagttt tgcataaata gcagaacctt taggcataga atagtgctga
     2701 ttgtcaaagc gataaagcgg tgtggaacca atagcttctg taattgtcga gataatcatg
     2761 tgggcctcct aaaaaaagat aataaaaaaa ggagctgact attaaatcag ctccttgaaa
     2821 gacatagaga aaaattgagt agataaaaag ataagttacc tggtactcat tctatgaagg
     2881 gcaattaata gacatgacaa atagtatgct gcttttatgc accatacaac agcaacgatt
     2941 caattgtttt tggttgctga ctgtcatgtg tatccctcca agtaaaattt ttattaacga
     3001 tagcatgaaa aaaaataaaa gtcaaaggct tgaaagaaaa aaatatttcg atagcatcgt
     3061 ttacccgtgt ttatttttct gacaaatagg catgagacct taaaagatag agaatactct
     3121 agttttctgg ccggttgtct gatacaataa agttgattat gataaagaaa agaaggagca
     3181 aaaataatga tctcagcaag tgatttaaga gccggtatga ctttcgtaca agacggcaaa
     3241 ctgattaaag ttctggatgc aagccaccat aaacctggta aaggaaatac agttatgcgt
     3301 atgaaactga aagatgtccg ttcaggtgca acatacgaca caacgttcag accagaagaa
     3361 aaatttgaaa aagcacatat cgacacaaaa cctgtacaat atttgtacac gatggatgat
     3421 acagctttct tcatggattt ggaaacatat gagcaatatg aaattcctgt agaaacagta
     3481 gaagaagaaa tgaaatatat cctagaaaac atggaagtaa aaatccaatt tttcggtagc
     3541 gaagtcatcg gcgttcaatt accaacaact gtcgtattac gtgttgtgga aacacaacct
     3601 tccatcaaag gtgcaacagt cactggttca ggtaagccag cgacaatgga aactggctta
     3661 gtagtcaatg ttcctgactt catcgaaaca gatgaattgt tggaaatcaa tacagcagaa
     3721 ggttcttatg taaaacgtgc gtcaaaa
//
LOCUS       DACEDM010000130         3732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEDM010000130 DACEDM010000000
VERSION     DACEDM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.139"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IWO50_002784"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IWO50_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0359846.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="IWO50_002785"
     CDS             1228..2331
                     /locus_tag="IWO50_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0359847.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IWO50_002786"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IWO50_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0359848.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
BASE COUNT         1131 a          748 c          634 g         1219 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aa
//
LOCUS       DACEDM010000131         3633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEDM010000131 DACEDM010000000
VERSION     DACEDM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.140"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..2376
                     /locus_tag="IWO50_002787"
     CDS             4..2376
                     /locus_tag="IWO50_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359849.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..>3633
                     /locus_tag="IWO50_002788"
     CDS             2437..>3633
                     /locus_tag="IWO50_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0359850.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAK"
BASE COUNT         1420 a          582 c          787 g          844 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgttacga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgag
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcatgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatt gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat aaaacagggc agacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaattttc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaa
//
LOCUS       DACEDM010000132         3514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEDM010000132 DACEDM010000000
VERSION     DACEDM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.141"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            71..397
                     /locus_tag="IWO50_002789"
     CDS             71..397
                     /locus_tag="IWO50_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0359851.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            398..1147
                     /locus_tag="IWO50_002790"
     CDS             398..1147
                     /locus_tag="IWO50_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359852.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(1227..1682)
                     /locus_tag="IWO50_002791"
     CDS             complement(1227..1682)
                     /locus_tag="IWO50_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0359853.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(2109..2384)
                     /locus_tag="IWO50_002792"
     CDS             complement(2109..2384)
                     /locus_tag="IWO50_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359854.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            2678..3493
                     /gene="thiD"
                     /locus_tag="IWO50_002793"
     CDS             2678..3493
                     /gene="thiD"
                     /locus_tag="IWO50_002793"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0359855.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         1164 a          530 c          689 g         1131 t
ORIGIN      
        1 tactttacct gatgaagtaa tcatgctatt ctaattacaa cgacagatgg agtaatatgg
       61 aggaaagaaa atgatttcaa gtgatggaat acgcggatac aatgatgcga ttattttatc
      121 gatccttaac aaaaacgatt cttatggata tgaaatttct aaaacgattc gtgagaggac
      181 aaatgattcc tatgtgatca aagagacaac actttactcc gtttttgccc gtttagaaaa
      241 gaatcaactg attgtttcgt atcctggaga agtgactcat ggaaaaaagc ggacatatta
      301 taaaatcact gaaatagggc gtaatctttt agaagaaaaa aagcaagagt ggctgacgac
      361 gaaagacgta gtcgatcaat ttttggaggg aaattaaatg gatacaatcc gtaattattt
      421 agacagtctt tttatcggag tacctcaaag taccgagata gataagctga aaactgattt
      481 gttggcaaat atggaagacc attatcatga attgatggga gaaggaaaaa atgaacagga
      541 ggccatcggt acagtaattt cgacatttgg ttcaatcgat gaattattag aagaactaga
      601 tgtcgaaaaa aaacatcaag ctgacgagac ggaaacaaac acagcttcta tttatttaag
      661 cgaagcagaa aactactgga aagaatacag agcggcaagt ctccaagtgg catcaggtgt
      721 tctattcatt agcttatctt ttgcaagttt tcttttcttt tgttcagcag gatatgtgtt
      781 catgggtatt tcttgtctga ttttcggtat agcattagct gtcggttttt ttattgcttc
      841 tggtatgaaa atcacacgtt taaatcactt tttgcaccat cggaaaatac ctgaaaaagt
      901 attagcagaa gcaaaagaaa aagaagaaga gtatcagcga agttttggct tctcactgat
      961 tgcaggaatt ggattatgta ttttttcact tttcccactt ttggcttctt taatgtggta
     1021 tatggatggt agtatcggtg cctctatatt ttttgttacg gtaggaacgg gtgtcttctt
     1081 gatcatttat ggcagtctag tccgtcattc ttatcgtcaa ttcactcaaa gcgcttatta
     1141 ctggtgaatg tattcagatt tgtaaaaata ttctatttat ccggcgtaga aaaaaagact
     1201 gtgacacgct tttgtcacag tcttttttat atataataat ttaaaaattc atcatctaaa
     1261 tccgtagaat tttctagatt gcttacccat gtaaaatcgt tatagacaga tttcagttga
     1321 cttctgcgcc attgtacagg gataaaaaga cgcatgctat cttcattgaa tccgactaat
     1381 aatttctttc cgtcagtaat gatgggacgt tgtaacaaag taaaatgcga gacgatcaga
     1441 tcgacaagct ctgaggtcgt acattggtcg atttggtctt tcaaattttt ataatcaatc
     1501 gagcgtttag ctaatagatc gtcaaatccg ttttctgtta atgacaataa ataaaatatc
     1561 tctttccttg ttaaaggctg tttaggtgtg aaaattcgtt ctctgtaagg catttgatgg
     1621 tctttcagcc agtttgccat cagatggtaa ctatgtgtgc gagaagaata gatcgtaagc
     1681 ataaaaaaac tccttaaata gtccattaat ttgttagatc tgttgagata aataataggt
     1741 aaggagctgt gcataagcat tgatcaaaac aacccaaaca tcattagaaa taactttcta
     1801 aagtcttaaa cttgcgacga gaaacaacta gactaataga aaaaatgtct tagccatttg
     1861 gcttcctgtt tgtttttttg cttatgctgc agctcctcgg gaaagggttc taacaatttg
     1921 aagaaggttg atgattgatc tagtttaaat tgtgtgaaat ttgaaatctg gtgtttttac
     1981 tagatgaatc acctaacaac tatgattata acttatagaa cttctgtttt gcattcggtt
     2041 ttgttatagt tgttataaag ttttatttta ttacagaaca tttttaatta ataaagaaca
     2101 gttagaagtt aaaattgctg attcacatag agtttatgac ttcttggaaa ttctttttca
     2161 atcagttttt tcatctgttt atgaaattct tttttttcag taaacataaa ggtttgggta
     2221 tcgtaacctt cttcatgtag gaaatagatt attccttttt ctgtgttttg cgtataagct
     2281 tttatactcc tgtctttttt gaaagaacag atcgtatagc catctatttg gtctggtagt
     2341 cgtttgattt tcttcaaaat gactttttta tcctcaagtt tcattttttt cacctgactt
     2401 tatttttttc atatggaaat catagcgcct ttggttatat gaaaacaaga aggatactga
     2461 aatatatata aatcaaatag tggttattta ttattagaat agcgaataca aataaacgac
     2521 cactattttc tgatttattg caaatgtttt ttcaaataaa tttgattacc aagaaaaggt
     2581 agtggtatat ttactgaggg atactttgaa aaacatatga tagaaacagt aacagtatta
     2641 tgaaaaatct ttttataaaa atggaggaat cgatttgatg gaagatcagt atgtacaagc
     2701 aatgacgatc gcaggatcag atagcgatgg aagcgcaggg atgcaagcag atatgcatac
     2761 attctttaca agaaatgttt acggtgtttc tgtcatgacc gcatgtgttg caggtaattc
     2821 ttacggaatc ggagcaagtg tgacattgcc aacagatttt atcgacaaag aatttgaact
     2881 gattgcgaag gactttcaaa tacgtgcagc taaaacaggt atgctagcag attcaaagtt
     2941 gattgagaca gttgtaaaaa attaccaaaa atatgatttt ggaccattag tcgtggatcc
     3001 cgtaatcgta acgaaacatg gaaatctttt actagaagaa tctgctttgc aaagcttgaa
     3061 agaaaagctt gtccctttgg ccgaagtact gacacctaat ttttatgaag cagaaaagct
     3121 gactggacgg acattagcag ctgaagaaga ttttgtgcaa gcagcaaaag aactgcaaga
     3181 tatgggtgca aaaaacgtta tgatcaaagg acatcatgct aaaaatgcgc agcaagaaac
     3241 agtcaatgac tacgtattgt tcgaatcagg aaaatcattt tggttaaatg caccatttta
     3301 tcctaccgac cggatcaacg gaactggcga ttcgctatct gcatgtattg cagctgagtt
     3361 agcgaaagga aaaacagttg aacaagcgat ccgattggca aaagactatg taaaccgtgc
     3421 aattcgtgaa gaaataaatg tagggcacaa atatggcccc atcaatcatt gggcagctgg
     3481 tgaattagag taaaagaaag aagttgtgaa aaag
//
LOCUS       DACEDM010000133         3281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEDM010000133 DACEDM010000000
VERSION     DACEDM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.142"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..585
                     /locus_tag="IWO50_002794"
     CDS             1..585
                     /locus_tag="IWO50_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0359856.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(662..1837)
                     /locus_tag="IWO50_002795"
     CDS             complement(662..1837)
                     /locus_tag="IWO50_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0359857.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1919..2299
                     /locus_tag="IWO50_002796"
     CDS             1919..2299
                     /locus_tag="IWO50_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0359858.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2373..2735
                     /locus_tag="IWO50_002797"
     CDS             2373..2735
                     /locus_tag="IWO50_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359859.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1114 a          538 c          622 g         1007 t
ORIGIN      
        1 atggcattcc cacaactaga tttaaaaagt gaaaaaggac caaaagctgt tatcaaaaca
       61 aaccgtggtg acattactgt tcaattattc cctgaactag caccgaagac ggttcaaaat
      121 ttcattgaac tttccaaaaa aggttattat gatggcgtga tttttcaccg cgttattcct
      181 gatttcatga tccaaggcgg cgatccaact ggaacaggaa tgggtggcga aagtatttat
      241 ggcgaaagtt ttgaagatga attcagtcgt gaactgttca accttcgcgg agctttatct
      301 atggcaaatt caggtccaaa cacaaacgga agccaattct ttattgtaaa taacacaaat
      361 gtgccagcta atatgcttgg acaactagaa ggtgctggat tcccatcaga aatcattgaa
      421 gcgtataaag gtggcggaac tccttggcta gatttccgac atacagtctt tggacatgta
      481 ctagaaggga tggacacagt agatgaaatc gcaaatgtac aacgtggtcc acaagaccga
      541 cctgtacatg atgtagtgat tgaaacaatt gaaatttcag aatagtttgg tatggtacgg
      601 agtaattatg aaaaaggacg aaacctagta aggtctcgtc cttttatttt gaataaatga
      661 tttaatcgat tgtctctaaa gcatacttga ttcgattaag tccttccagt aaagtttctt
      721 ctggacaagc aatattcaag cgcatatgcc catgaccgga aggaccaaac gtgatgccag
      781 gatttaatac gacttttcct ttccgaacaa gagtgttctc caattcttgg tcgtctgaaa
      841 tataggcggt aaaatctaac cacattaagt aagttccttc agggttcatt attctcactt
      901 tcggaagata ctgttcaaaa aactgattaa ctgtttgaac atttttttct agataaggaa
      961 tcagttggct taaccattct ccacccgtct cataagccgc ttgggttcca atcagtccaa
     1021 aagtgttgat ttcgtgctgt tggttcatca ctaaatgttt ttcaaaatca gctttcatct
     1081 ctggattttt gataaatacc attgaatttt tgattgcagc tagattgaaa gtcttggttg
     1141 cggaagtaaa agcaactagt agatcatcta aattaggatc aatcgttagc atggaagtaa
     1201 atgtatgatc gaataaagtt aaatcttgat ggatctcgtc actaaacaga atcacttgat
     1261 gttttaaaca caattcgctt aactgtttca attcttcttt gctccaaaca cgccctccag
     1321 gattgtgagg attgcataaa atcattgctt ttacattgtt ttcttcgatt tttttctcca
     1381 tgtcagacaa gtccatgatg aaatgattat ttttttctag caaagagctt cgaacgatgt
     1441 gacgttgatt tgtttctata atagaggaaa atggaggata aactgggtca tgaatcaaaa
     1501 tagaatcgcc tggttttgta aaagtttgta ctgctgcagc taaactggtt aacacaccac
     1561 ttgtaaaaac gatattttct ttcgtcaact ccacagcatg gtgttggcgt tcccaatgaa
     1621 tgatagcctg atatagttta tcagacagag tggcataacc aaagattcca tgtttgatgt
     1681 attcactaaa cgctttagct accccaccgg gagacaagaa gtccatatct gctacccaca
     1741 aaggaagtaa gtcatccatt tgatatgtct cagcaattgc gtcccatttt acactttctg
     1801 tatcttttct tgaaatctgt ttatcaaatt cgatcatctt aagacctcca gtcattttcg
     1861 tcgcttcttt ttttgagcta tttacagtat aatgataaat gcgaagagag ggaagtagat
     1921 gacttataaa attggacaaa taattgaagg aaaagtgaca gggatacagc cttatggcgc
     1981 attcgtttct cttgataacg agacgcaagg acttattcat gtttcagaag tacaatccgg
     2041 ttatacgaaa aatatccatt cattgttaaa agtcggacag ccagtaacag ttcagatcat
     2101 cgacattgat gaatattcca aaaaaatcag tttatcttta agaacgttag aaaaaacaaa
     2161 tccagctgtt ccatatcgca gaaaaaggta tttcacgaat aaaaatcgta agatcggatt
     2221 taccactatt gcggagcagt tacctatttg gatagaagaa gcattatctg aactagaacg
     2281 gaaagaaaat aagaaataaa aagtgtattt tttctgaaaa tgtgctagaa tttttatgta
     2341 agccatagaa caagttgatc gaggtggaaa ttttggcaag caaaagagtt gctggtacaa
     2401 ttatgttgca tttggaggat ggtactaaaa aatttttggt ccattcaatt gatgacaagt
     2461 tggaatttgc gttagcggaa ctttctgaag gaaagactgg tttagcgaat atacttaatt
     2521 tcttaaaaga aatagttcat atcgatgtta gcaagattag tttgatggaa ttgacaaata
     2581 cacatattaa agaggaaaat gttccgttgt ttgtctttga aactgaacaa aagaatcaaa
     2641 gagaagaatt aggcgaagga tatatttggg aagaaccagg tcaaatgcgc tctgttcttg
     2701 gaacttacga agtagaggga gtgcctttct tctaaaatac agattaagcg aacaaagaag
     2761 tttgaatttt tttcaaactt ctttttttat tgtttggtcc aaaaaataca atgaaatgtt
     2821 ctgctaaaga tagtttttta gtgcatttta ttttcaaaat atgttgaaaa cggttgtata
     2881 acgaataata ataattgatc gaaaaatcat ttcacaaagt aatgcttaat ttatagttga
     2941 ccaaggaaga aatacatgat aaatttaaca gcgttgattc tgaagtggat gttatatcac
     3001 atttctaaaa aatttaaaaa atagttattg acatgttact aacgacatga taagataact
     3061 gagtcgttaa aaaagaacca cttgcgaagc ttgattctat cgaaaaaaac ttcaaaaaag
     3121 ttcttgacaa ataacaaacg ataagttatg ataaatgagt tgctgaaaga tttcgaaaaa
     3181 cgaaatcgaa aaaaactttt aaaaaagagt tgacaatcgc ttgtctctct gatatgatat
     3241 aaaagttgct acggcagcca aaaacgaatc gggaaacact c
//
LOCUS       DACEDM010000134         2961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEDM010000134 DACEDM010000000
VERSION     DACEDM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.144"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            138..575
                     /locus_tag="IWO50_002798"
                     /pseudo
     CDS             138..575
                     /locus_tag="IWO50_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(652..2199)
                     /locus_tag="IWO50_002799"
     CDS             complement(652..2199)
                     /locus_tag="IWO50_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0359860.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(2301..2654)
                     /gene="tnpB"
                     /locus_tag="IWO50_002800"
     CDS             complement(2301..2654)
                     /gene="tnpB"
                     /locus_tag="IWO50_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0359861.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2644..2838)
                     /locus_tag="IWO50_002801"
     CDS             complement(2644..2838)
                     /locus_tag="IWO50_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359862.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          871 a          607 c          539 g          944 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cacaacattg aaaaatctgg ttttatcgtg tatgattagg gtgtatttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcagt gagaattttc atcgagaaac
      241 aatcgggcgc aacgattact catcgacctg ttttccaaga agcattggat ttcgtccgag
      301 atcaagacat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattaccta aagaagaaaa acgtacagct aatcatcacc agtctaccga
      421 tcatggctga agcaattggc aaccctctcc tggataaatt cattaaggat ctgatcatcc
      481 aaattttggc catgatcgct gaacaagagc gtaccgaatc taaacgacgc caggcgcaag
      541 gcattaaaat tgcgaaagca aatggagttt ataaagtaag cgccccatag aacagtacct
      601 gtacgtcaaa taacccgacc tatagaatag aatcgggaat gtttggcgtt tttagttacg
      661 agaaaaatgt tgttgtacat ccttgttcca aggcaacagt tgatctaaag tttctgctga
      721 aagtacggga agatttggta tcttatcaaa tagaaactct agatacttta caggattcaa
      781 tccgttaagt tctgctgttt tatatacact cagaatgatt cctgaagttc gtgctccttc
      841 aagacttgtg gaatgcaacc aatttttccg tcctatcacc agctctttga tatttcgctc
      901 cgcacgatta tttgtgagtt ctaaacgacc atcttcaaaa ataggttcaa aaccacttcg
      961 tagtttacac gcatattgaa cggctgcatc caacttggat tttgcaacag gatctatcgt
     1021 tgccatccac tcataaaaag caataaataa tggacgcagt tcttgttgcc gacagcttaa
     1081 tcgcttctca ggagaaaagt ctttccattt cttttctagg acaaaccatt tatccaattg
     1141 tttgactgct tgagctgcgg gaatatccgt gtttttcttt ccttttggaa ttgcttcgaa
     1201 aaatttccgc cgaacatgcg cgaaacaata gacaagtcgt acactttcca cggcattata
     1261 cccgctatag ccgtcacagt gtaaataccc tgtaaactcc ttgagaaaag ttttcggaac
     1321 atctccagca cgactttcgt cgtggtgata ataaacgatg ggatgctgac tgtcttttcc
     1381 tgtacaaaac tgccagtaat aggtatctgt tttggcactt tcaatcacac gataacttgt
     1441 ttcatccccg tgtagaatct cttcttgatt aagatagact cttaatcgtt ctcctaaagg
     1501 gtctaacgcg ttttgcactg ctttaatatg ccaattggtg atattatctc tcgaaatatc
     1561 caacccatgt gtttgtttcc aatagttctc ttgccgatag gcaggaactt tctgaccaaa
     1621 tttcatccgg atcgtttccg ccactaaact attggaacct aagctattag aaatcaaagg
     1681 tttaggaacg atcgcttttt taaaagaaga aagtccttct ttttcgcagc ttttacaaca
     1741 ataggtatgt tgataatggg tgagacaatc caacatagcc tgatggaaac gaatttcttc
     1801 tcgtactttt gtccgtccga tatctttcat ctgtgaaccg caatgttcac aaaattgttc
     1861 ttccaaagga aggagatgtt ctttctctgt tataggtaag tgtttgattt tgtctgcttt
     1921 cgttcctaca actcgttgat gtccttttac tggaataatg gtggcagctg cttctggaac
     1981 acttacagaa atttcttcat cactaaaaag attcaattgc ccattcgttt cctcaaagag
     2041 agtctcttta gagcgtccaa ataatttgtt ggttaaataa ttgacctttt cattcaatag
     2101 ttggatttca gttgtaagat tctgaatggt ttgttgttgt gcttgattgg ttttatgggc
     2161 ttcttgaagc aactgaagca acgtatctgt agtttccaaa atttttcacc accttgaatc
     2221 gaactgattt tgttacagaa taccacaaga aacgcacaag aaataggggt tcaacacatt
     2281 ttttgtgtta accccccagc ttaaaaaact cctttttttc ctggcaaaat agctcgaggt
     2341 tgatcgatac ttaatccttc taacaaccat cgaacctgtt ggttcgttaa ggttcgaact
     2401 tcgtctttcg tccgaggcca ttttaacttg ccgttttcta tccgcttata acagagaaga
     2461 aaaccatcac cgtcccaata gagaagtttg aaacggtctt gacggttccc gcaaaataaa
     2521 aaaacggcat cttcgtaaag ctcgagttga tactcttgtt gaataagact ggcaagacca
     2581 tcaattcctt ttcgcaaatc tgtttttccg cacacaatat aaatatgttt ggtttgaagt
     2641 aaatcataaa gcattggaca acagttcttt caaaatagct tgaagaatat aattgttaat
     2701 accgttatag atcaaaactt cagaaccatt ctctcttttg atgcgcatcg ctaacttggc
     2761 agccgcgggt ttaccttttt ctggtgcact cttttcgttt cctaattgga caggaacaat
     2821 ctcatttaat ttatacatag aaaacctcca gacttcttga tagctcaagt atatcttgag
     2881 gtgcatttgt tagtaaggta ctgttctatg gggcgcttac tttataaagg tcgtccaaaa
     2941 ctctatagtg cagatacaaa a
//
LOCUS       DACEDM010000135         2877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEDM010000135 DACEDM010000000
VERSION     DACEDM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.145"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..503)
                     /locus_tag="IWO50_002802"
     CDS             complement(<1..503)
                     /locus_tag="IWO50_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0359863.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLS"
     gene            complement(514..1890)
                     /locus_tag="IWO50_002803"
     CDS             complement(514..1890)
                     /locus_tag="IWO50_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0359864.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(2019..2666)
                     /locus_tag="IWO50_002804"
     CDS             complement(2019..2666)
                     /locus_tag="IWO50_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0359865.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(2750..>2877)
                     /locus_tag="IWO50_002805"
     CDS             complement(2750..>2877)
                     /locus_tag="IWO50_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359866.1"
                     /translation="SEWVKAGRIPDDEMYLSQIVENICYRNAYNYFNFFEIESRK"
BASE COUNT          954 a          579 c          347 g          997 t
ORIGIN      
        1 gataataatt catgtctttc ccattcttct tgaatagctt ccttattagc tgagcttaca
       61 ataattatct tcccatattt tttcagagct tttatagtat ctctagcact taaaaagggt
      121 ttatctaatc ctttagcttg actaatctgt cgattgactt cttccgacca ttttaaagct
      181 tttaataaat cctctgactg agagtattct atttccctct tcaaagatgg attagacaat
      241 tcttttgtta cagataccca gtgctttaat cgattaatcg atggtacatc atttttttct
      301 aatgttttaa ctaaacctaa aaatctgttt attccacgag tctctgaata taaattaata
      361 ttattccaat cctgtaggaa tttttcccta tgatgaacat caaaaatctc tgcagcaatt
      421 ggcccgaaaa agagattgtg tttataagtc attgtatcca tgacacaacc atctgaatca
      481 atacaaaata tccaactgtt catgtttttt ctcctagtta tgtttttgat ttagcaaatt
      541 ataattttct aatacttttt ctttagataa tggatatacg aaaaataaaa tcaatcctac
      601 aacaaagaaa caaactgctg gaatcaacgt tgaagagtta tacagctttt gtaaaacttc
      661 tccactttgt acttttgcta acgaatcata ccctattata gaaagagtcc aacctccgac
      721 accgccagct aaagcttgtc caatttttcg ggcaaaagaa tatactgcat aaatagttcc
      781 atcttctctt tggccagatt tcacttcttg atcatcgatt acatcagtaa tattcgccca
      841 aatgactgta ttaaaaatac ctaaccccat atatccaaca gaagtaagaa ctaagaatac
      901 atacatatta ctaggcttta tcaaaaataa aagtaaatat acaatagcag agaagaacat
      961 ccctactgac gccaattctt ttttaccgta ttttttactg aaaattaaac ttacaggtga
     1021 aacaagtacc aacatcaaaa taggattgat aaagttcatt aaaactattc cctgtgcatt
     1081 attataataa tctggaaata cataattgtt catactcatt gttaaaagct gagcaagcaa
     1141 caaaaaaata gctgcagcta ttatagaaag caacgctcta tttttagcta atccttttaa
     1201 agatttcaac aatgaaactt ttaactgcga ttcatcactt ttaacccgtt ctacagttaa
     1261 tttataacat attacgtaac aaataactgc acacactgaa aatactcctg ccacaattgt
     1321 aaaagttgat ccaccacgta caatttgagc tccagaacta tctcttgtat aaataaaaat
     1381 tggtgttaac acgccaatga acatacctgc taaggtagct cctatactac gaaatgttga
     1441 taacgctgtc ctttcttctg caacagaagt gatagctgat gccattgaac cgtaaggaat
     1501 attaatagat gtataaaaaa tacttcccca tagtatatat gttacataca tatatataac
     1561 tttaatactc atagaagctg atgccattga tgtttgatac attaaaaaac ttgctaaagc
     1621 gactggacca ctcatccgta aaatccacgg tttaaatttg ccagttttag atggttttga
     1681 tccatccaca atacgcccca ttgttatgtc tgtaactgca tcaataaatc ttgctactaa
     1741 aaataatgtt cctacggtag ctccgcttat ccccaatact tttgtataaa aaatcattaa
     1801 aaaagaacta gcaaaaatga aagtgaaatc attcccaaaa tctccaaaca tatatcctat
     1861 tttgtctctt agaccaaatt ttctttccat ataatttgcg ctccttccta ttgttaagcg
     1921 ttttcattct gttgaactgc caacttacgt actatactct gaagtataaa gagttagtat
     1981 ttatttttta cattttatag tattatttat tttttctcct aataattctt tatctcataa
     2041 aacttatcta tatatttact agctattcga gttacttctt caaaatcacc tttttcaaca
     2101 ggagccaaca aattcccacc tacaccaact gcaacaacgc ctgcttcaaa ccactcttct
     2161 aagttatcta gacttactcc gccagtaggc attatattta catatggtaa tggagcttga
     2221 tacgttttta caacagatgg accaaaagca gaacctggaa atagtttaat gatttctact
     2281 ccagcggtaa gtgcctcctt tatctcagta attgtcatac aacctggtaa ataaggaatt
     2341 tgatataaat tacaaatctc tgctgtttgt cgatcaaaag atggacttac gatatattct
     2401 gctccagcca taattgcaat acgagcagta aacgcatcta aaactgtacc ggctccaatt
     2461 attacatctt tttgatcagc atatttttta gcaatttctt tgattatttc ttcagcattc
     2521 ggaactgtaa aagttagttc aatcccttta attccacccg cagtaattgc atttattgct
     2581 ttaatggcct catctttatt ttttccacgt acaaccgcaa tgactccagc cgcttctaac
     2641 ttacttaaaa tttctacttt tttcatatct atctccttct tttatggata catactctag
     2701 ctagttgtac taacttacaa taaataaatt taagctttta ttttttgtat tattttctac
     2761 tttcgatttc aaaaaaatta aaataattat acgcattacg ataacaaata ttctccacta
     2821 tctgactaag atacatttca tcatctggaa ttcgacctgc tttaacccac tcgctta
//
LOCUS       DACEDM010000136         2780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEDM010000136 DACEDM010000000
VERSION     DACEDM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.146"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IWO50_002806"
     CDS             <1..197
                     /locus_tag="IWO50_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359867.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IWO50_002807"
     CDS             314..811
                     /locus_tag="IWO50_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0359868.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="IWO50_002808"
     CDS             786..1292
                     /locus_tag="IWO50_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0359869.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..>2780
                     /locus_tag="IWO50_002809"
     CDS             1276..>2780
                     /locus_tag="IWO50_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0359870.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          515 c          600 g          826 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaagc
//
LOCUS       DACEDM010000137         2779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEDM010000137 DACEDM010000000
VERSION     DACEDM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.147"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..415)
                     /locus_tag="IWO50_002810"
     CDS             complement(<1..415)
                     /locus_tag="IWO50_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0359871.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIK"
     gene            complement(425..1801)
                     /locus_tag="IWO50_002811"
     CDS             complement(425..1801)
                     /locus_tag="IWO50_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0359872.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(1921..2568)
                     /gene="eda"
                     /locus_tag="IWO50_002812"
     CDS             complement(1921..2568)
                     /gene="eda"
                     /locus_tag="IWO50_002812"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0359873.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2652..>2779)
                     /locus_tag="IWO50_002813"
     CDS             complement(2652..>2779)
                     /locus_tag="IWO50_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359874.1"
                     /translation="SEWVKAGRIPDDEMYLSQIVENICYRNAYNYFNFFEIGSRK"
BASE COUNT          945 a          551 c          336 g          947 t
ORIGIN      
        1 cttttatagt atctctagca cttaaaaagg gtttatctaa tccttttgct tgactaatct
       61 gttgattgac ttcttccgac cattttaaag cttttaataa atcctctgac tgagaatatt
      121 ctatttccct cttcaaagat ggattagaca attcttttgt tagagatacc cagtgcttta
      181 atcgattaat cgatgttaca tcattttttt ctaatgtttt aactaaacct aaaaatctgt
      241 ttattccacg agtctctgaa tataaattaa tattattcca atcctgtagg aatttttctc
      301 tatgatgaac atcaaaaatc tctgcagcaa ttggtccgaa aaagagattg tgtttataag
      361 tcatcgtatc cataacacag ccatctgaat caatacaaaa tatccaatta ttcatatttc
      421 tctcctattt atgtttttgt tttaaaagct cgcaattttc taagacttta tctttagata
      481 atggataaag gaaaaataaa attaatccta caatcagaaa acaaattgat ggaattaatg
      541 ttgaagcgtt atataacttt tctaaaacat ctcccgtttg tacatttgct aaagagtcat
      601 agcctataat agaaagagtc caacctccga cgcctccagc tagagcttga cctatttttc
      661 tagcaaaaga atataccgca tatacggtcc catcttctct ttgatcagac tttacttctt
      721 gatcatcaat aacatcagta atatttgccc aaataacagt attaaacaca cccaaaccca
      781 tatagccaac cgaactaaga attaagaata catacatatt actagttttt atcaaaaata
      841 agatcaaata tactattgct gaaaagaaca ttcctactga tgccagttct ttttttccat
      901 attttttact taaaaacaaa gttaaaggtg aaacaactat taaaaccaaa ataggattga
      961 taaaattcat taaaactatt ccctgtgcat cattatagta atcaggaaat acataattat
     1021 tcatactcat aattaataat tgagcaagga gtaagaaaat agccgcacca attatagaaa
     1081 gtaatgccct atttttaact aaccctttta aagattttaa caatgaaact ttgtattgag
     1141 attggtcaat tcttactcgt tcagtagtta gtttataaca tattaaataa caaataagtg
     1201 cacatatcga aaatattccc gcaacaattg taaaagttga tccaccacga actatttgag
     1261 cgcctgaatc atctcttgta taaataaata atggtgttaa tacaccaatg accaaacttg
     1321 ctaaagtagc tccaacattt cggaatgtcg atagcattgt tctttcttct gtaatagaag
     1381 taatagctga tgccattgat ccataaggaa tatttataga tgtgtaaaaa atactccccc
     1441 ataatatata cgtaacatac atgtatacta ccttaacgct catagaagct gaaatcatag
     1501 aagtttgata cattaaaaaa cttgctaaag cgactggacc actcattctt aaaatccacg
     1561 gtttaaactt accctgctta gttgctttcg atctatcaac aatacgcccc atagttacat
     1621 cagtaactgc atcgataaat cttgctacta aaaataatgt tccaacggca gctccactta
     1681 tccccaatac ttttgtataa aagatcatta aaaaagaact agcaaaaatg aaagtaaaat
     1741 cgtttccaaa atctccaaac atatatccta ttttgtctct tataccaaat tttctttcca
     1801 tacaattctc tcccttctgt tgttaagcgt tttcattctg atgaaagtac gtactatact
     1861 ctgaagcgta aagagtcagt atttattttt tacatttcag agtattattt gttttttctc
     1921 ctaataattc tttatctcat aaaacttatc tatatattta ctagctactc gacttacttc
     1981 ttcaaaatca cctttttcaa cagaagccaa taaattccca cctacaccaa ctgcaacaac
     2041 tcctacttca aaccaatctt ctaagttctc tagacttact ccgccagtag gcattatatt
     2101 tacgtgtggt aatggagctt gataggcttt tacaatggat ggaccaaaag cagaacctgg
     2161 gaatagttta atgatatcta cgccagcagt aagtgcctcc tttatctcag taattgtcat
     2221 acaacctggt aaataaggaa tttgatataa attacaaatc tcagctgttt gtcgatcaaa
     2281 agatggacta acgatatatt ctgccccagc cataattgcg atacgagcag taaacgcatc
     2341 taaaaccgta ccggctccga ttattacatc tttttgatca gcatattttt tagcaatttc
     2401 tttgattatt tcttcagcat taggaactgt aaaagttagt tcaatccctt taattccacc
     2461 cgcaataatt gaatttattg ctttaatggc ctcatcctta tttttcccac gtacaaccgc
     2521 aatgactcca gccgcttcta acttacttaa aatttctact tttttcatat ctatctcctt
     2581 cctttatgga tacatacttt gtctagctgt actaacttac aaagaataaa tttaagcttt
     2641 tatttcaagt attattttct acttccgatt tcaaaaaaat taaaataatt atacgcatta
     2701 cgataacaaa tattctccac tatctgacta agatacattt catcatctgg aattcggcct
     2761 gctttaaccc actcgctta
//
LOCUS       DACEDM010000138         2630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEDM010000138 DACEDM010000000
VERSION     DACEDM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.148"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IWO50_002814"
     CDS             complement(61..2424)
                     /locus_tag="IWO50_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ0359875.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT          700 a          553 c          483 g          894 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt
//
LOCUS       DACEDM010000139         2623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEDM010000139 DACEDM010000000
VERSION     DACEDM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.149"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..842
                     /locus_tag="IWO50_002815"
     CDS             <1..842
                     /locus_tag="IWO50_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0359876.1"
                     /translation="PKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTD
                     TRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGT
                     VVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGS
                     QHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKG
                     SKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKK
                     ENE"
     gene            976..2319
                     /locus_tag="IWO50_002816"
     CDS             976..2319
                     /locus_tag="IWO50_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0359877.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2431..>2623
                     /locus_tag="IWO50_002817"
     CDS             2431..>2623
                     /locus_tag="IWO50_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0359878.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSE"
BASE COUNT         1044 a          339 c          478 g          762 t
ORIGIN      
        1 taccaaaagg aactacttat gcttggaaag agaaagtaga tgttagcaca gctggaaata
       61 aaaaaggtac tattgtagtg acatatccag atggatcatc tgatgaagtt gaagtagacg
      121 ttacagtgac agatactcgt tccgatgcag ataaatataa tcctgaagga caaaaagtga
      181 ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatctac
      241 caaaaggaac tacttatact tggaaagaaa aagtagatgt tagtacagct ggaaacaaaa
      301 aaggtactgt agtagtaaca tatccagatg gatctaaaga agaagtagaa gttactattt
      361 ctgtagtaga taaaaaagca ccgaataaac ctcaagttga tcctattaca gagggtgacc
      421 aaattgttac tggtaaaact gaaccaaatg cagaggtgac agttacatta ccagacggaa
      481 gtcaacacca tgggacagct gataaaaatg gtaattttac agttaaagtt cctaaattag
      541 aggcagggac aaaagtgata gtaactgcaa ctgatgaatc tggtaatact agtgaaccta
      601 ctaatgtagt tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt aaaggtagta
      661 agactgacaa tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa agccagtcct
      721 caaaagtttt tcctaagacg ggagagagtg acagtaatat ctttactatt agcggtggtt
      781 taatactgtt aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa gagaatgaat
      841 agaaaatcaa tttcttaagt cttctctgag aagaggaaga atctttttct tcctcttttt
      901 gcttttttat gatgggaaat aagtgttagt taaagatgaa tatacttttt atagaaagga
      961 aaagaatgta attaagtgga aaaaatagta attattggtt caaaccattc agggctagcc
     1021 gcagctcgat ttctaaactt aagcgattca aattatgaag ttattctaat cgataaaaaa
     1081 tcagaaatta gttacttgga atgtgggaca tcgttattta taaaaggaga agtaagtaat
     1141 ttagaagatc ttttttatca aaatagaatg acactaaatt ctgagataaa aaagttattt
     1201 ctagagacag aagttgacaa aattgatatt caaaataaaa agattagttg tgtaaaaaaa
     1261 gatggtacaa gattctctca agaatatgac aaagttatat tagctacagg ttcaaagcaa
     1321 attatgctag atataccggg gaataattta gctaatattt tttcgataaa gaattttcca
     1381 aatgcaaaag agatttttaa agctttggat gatagtacta ttaaaaaagt aggaattatt
     1441 ggagcaggat atattggagt tgaaattgct gaagccataa gaaaaagagg gaaagaagta
     1501 tatcttttcg atgttgcaga tagagtgttg tcgacttatt acgaccgtag tttttcagat
     1561 aaagttgaag aaatactatc aaaaaatgga atacatcttt gtctatcaga aagtcctaaa
     1621 aaatatttag gaagaaaaaa gattgaaaaa atagtcacta caagtggtac ccaatactct
     1681 atggatatgg tcgtacaagc tataggattt ctatctaata gtccaattgg ggaaaaggaa
     1741 ctcttgagag atgttagtgg agcatatcta actaatgaat accagcaaac aaatataaaa
     1801 gatatatatg ctattgggga ttgtgctaca acttttttta cgtccattaa tagaagtagt
     1861 gttgattttt ctatttctaa tgctctaaga acagcttata ttgcaactca acatattaac
     1921 aatcaagatt ttacaccttc tggttcccaa tttacaaatg gcttttctat atttaattac
     1981 aatttttacg cagcaggttt gaatttaaaa acagcacgat tattaagtga gtctatagat
     2041 tatatagaaa tagaagattt cattagacct gcttttttga gaaaaaatag tcgtgtaaaa
     2101 ttaagaatta gctttgaaaa aaatacaaag agaatagtag gtgtacaact ttgtagtcaa
     2161 gaagatttat ctggactatt atcaatgttt tctcttgcta tagaagaaca aattacatta
     2221 gacaaattga aaatgttaga ttatttattt catcctagtt ttagccaacc ctataatttt
     2281 attttaaagg cagtaacagg attaaacatt gaaacataat cgttactaaa tgaaatcaag
     2341 aaatatactg tattactcaa ttcattgaaa atatttatca taatttttaa tagaaaacac
     2401 tattaaagag aaaaaagaga ggtaaatcaa atgaaaaaat ctttaaaact tgcatcagca
     2461 gttatattaa tgtcccaact ccttatagca acacctttcc atgtgttggc aagcgaaaat
     2521 tcaacataca aaattgcaaa agaaagaacc gagacgactg aaatgagtaa ttccgatgta
     2581 acagcagaga gtagtgaact tttgacaact tcatcaagtg aaa
//
LOCUS       DACEDM010000140         2614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEDM010000140 DACEDM010000000
VERSION     DACEDM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.150"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..318
                     /locus_tag="IWO50_002818"
     CDS             <1..318
                     /locus_tag="IWO50_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0359879.1"
                     /translation="KAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFIG
                     KRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITHL
                     VKK"
     gene            732..1550
                     /locus_tag="IWO50_002819"
     CDS             732..1550
                     /locus_tag="IWO50_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0359880.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1623..1982)
                     /locus_tag="IWO50_002820"
     CDS             complement(1623..1982)
                     /locus_tag="IWO50_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0359881.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          897 a          368 c          461 g          888 t
ORIGIN      
        1 aaagcaatca ttcctgagaa cttagatgag gttaaacttt atttatggga agataacgga
       61 gaaattgtcg gttttagtgg tgtaagtgca gaagaattag ttatgctatt tcttgatcca
      121 gattttattg gtaagcgata tggtagtcaa atattaattg agctgattga aactgaagat
      181 ataaaaagaa ttgatgttaa tagtcaaaat aaatatgcaa aacattttta tttaagccat
      241 ggatttgaaa ttgaatctga agaagactta gatggttttg gtaaaccata ccctataacg
      301 catttagtaa aaaaataatt catgaatggt ttattacctg attgaacatg aataaaagta
      361 atatttttat tttgatggcc tatttctagg ttatgaatta tccagatcaa atttgtgtat
      421 tacattcagt aataaggtgc ttatcttagg atcaacttat gatactagct gaccaaggat
      481 gtaccagtta tagaaaatca gttaaagtaa aaatgataaa aatcttatgt caacactgat
      541 ttaggctgac ctgtgggtta ggagaaagac aggtcggtag cttttaatac aaaaaatggc
      601 ttgtatgtat ctagatagtt aactgaaatt agaaatgttc ttatttttaa cggagtaaga
      661 aaaataggct gctttaggtc tgaatacttt ttcaatataa tgaagatatc aaaggcgaca
      721 ggagtgtttt tatgaataag aatcctcata atctaaagtt ggtagcagta gatatggatg
      781 gaacatttgc tcgtactgat tacacatacg atatcccaag gttcaaggct gttttatcgc
      841 gaatgaaagc tgttgattgt caatttgttg ttgcaagtgg aaatcaatac taccaattga
      901 gatcgttatt tccaaattat tatgatgaat taagttttgt tgctgaaaat ggtgcttttg
      961 ttaaagataa aacagaggta ctattttcag ccgatatgcc tagaaaagcg atcgaggtgg
     1021 tccttgattt atgtaaagac tatcctgaag taaaaaatgt gatgtgtggt ctgaatagtg
     1081 cctactgtga aagaggaaag gtgagtcaag cattttttga tctaaccaat atctattacc
     1141 atagattaga atgggtagct gattttgcag aagttcaaga taaaatttta aaatttgctc
     1201 cgactatgcc tgttgaaaaa accgaatttt acgttgaatt gttccgtgaa aaattagccg
     1261 ggctattagt acctaccagt agtggtcatg gttctgtaga cttgattctt tctgagtgcc
     1321 ataaaacttt cggcttgaaa atgttagttg agcgcttggg gattaatcct gaccagtgcg
     1381 ttgcttttgg agatgggggc aatgacattg agatgttgga gtattgtggt ctgagttatg
     1441 aaatggataa tgcaacggaa gctgttaagc aagttgctaa gcatcaatgt ccttcaaatg
     1501 acgaagacgg tgttttagtg acgctcgatc gattgttccc taacacgtaa acactaggta
     1561 attcatcaaa aaacctcgat ataagaaata ttcgaggttt taaaactata tttagaacag
     1621 atttacgaag cgtctgatga gtcaaaatag acttggacat tgctatctaa aataccactt
     1681 tcattcttta aacgaattgt ttgatttgat ggagttacct ctaatactaa atgcccatta
     1741 ataaaggtag tatcgtattc agatgtttta actgttgctt cttcgcaagg tattgttaaa
     1801 tcatatgctg gaatataatg ttgtgtggaa actaataaaa aatgatcacc aatcatacag
     1861 catccctctg ctgaagaaaa ttgattagag ccatcattta aagttaacaa gaatccatcc
     1921 tttaaacgag gtacttgctt aaatacttct ggttgtactt ctaaaaataa attatcgctc
     1981 attttcacga ccaccatcga cttccacagt tgaaattgac attttattta acacacctat
     2041 tcccccttaa ctataactca attctatata aaaaatattc tattctcaaa taattgaact
     2101 tttcaaatcg ccaatttact tcggctttat cagagctcat tcatatcaag agcgattaac
     2161 gctgtaaaaa ttaaaccgaa gatctgataa attttctcta ttttctacaa tatttaaaac
     2221 gcttccttag tctattatat atctatttct cttaggagta tgtttagatg tttgaataat
     2281 agttgcccaa ctttaaaggt tattcttata atattttact tcattttcca atagaaaatt
     2341 caaaggatta ctggtttttc taaaatagtt tagtttaaat acataagtat cctccgacac
     2401 tgtattagtt cataatcatc taaatacaaa attttcaaat aaaaatattt caagctctat
     2461 cgttgacgaa tgtaaacgat acttataaag taatagaaat tgattacatt tgtaaactta
     2521 aaatggttaa tttatctcac ataaaactaa gaataaaata atgattaaaa gtggttagtt
     2581 tttatacgat ttagcctatc ggtaaaaatg aatt
//
LOCUS       DACEDM010000141         2542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEDM010000141 DACEDM010000000
VERSION     DACEDM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.151"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            162..734
                     /locus_tag="IWO50_002821"
     CDS             162..734
                     /locus_tag="IWO50_002821"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0359882.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGW
                     CYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAR
                     EVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYI
                     EGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1207..1779)
                     /locus_tag="IWO50_002822"
     CDS             complement(1207..1779)
                     /locus_tag="IWO50_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0359883.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1795..2400)
                     /locus_tag="IWO50_002823"
     CDS             complement(1795..2400)
                     /locus_tag="IWO50_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ0359884.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          798 a          510 c          402 g          832 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccat catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct
      241 gtgagaaatg ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact
      301 tatcttcaat catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa
      361 tgactgtgga ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag
      421 aaggaatgat tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat
      481 ggcttaaaac caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg
      541 ctggaatcga atctttccat gcctcattga aaaaagaaga agtctacacg actagctact
      601 cagattttga agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa
      661 atcgaatcca tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag
      721 aaaaaatggc ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa
      781 tatcacaata ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat
      841 ttggttctta aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc
      901 aatagagtca atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg
      961 acttacgctg aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat
     1021 gatggtatcc taatatagta agcgtggcta gcataccttt aattacttta ccttttacag
     1081 ttaccataca cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac
     1141 atgctaatag actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact
     1201 tcttttttaa ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc
     1261 ggttatagct tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct
     1321 cttcttcggt gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct
     1381 tgcaaacatt tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac
     1441 actcaacaac gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt
     1501 caaaaccttg atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct
     1561 cgtgttacga gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc
     1621 agccaacagc tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa
     1681 aatattcttc ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg
     1741 agaagcggtt gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa
     1801 atcatctatc gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat
     1861 atataactcg cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc
     1921 caaatcgttt atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt
     1981 ccaatctaca actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact
     2041 tcggccattt ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat
     2101 ctcttcaaaa gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat
     2161 aactggtgca aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa
     2221 atcgtaaata tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac
     2281 ttcgatttca tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt
     2341 ttcttcaacc ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat
     2401 gcgaaatctc cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg
     2461 cgattttgtt tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg
     2521 tctctatttt gcacagtcta gt
//
LOCUS       DACEDM010000142         2452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEDM010000142 DACEDM010000000
VERSION     DACEDM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.152"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            68..280
                     /locus_tag="IWO50_002824"
     CDS             68..280
                     /locus_tag="IWO50_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359885.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            442..960
                     /locus_tag="IWO50_002825"
     CDS             442..960
                     /locus_tag="IWO50_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAQ0359886.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            967..1479
                     /locus_tag="IWO50_002826"
     CDS             967..1479
                     /locus_tag="IWO50_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359887.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1750..2373
                     /locus_tag="IWO50_002827"
     CDS             1750..2373
                     /locus_tag="IWO50_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0359888.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          939 a          305 c          416 g          792 t
ORIGIN      
        1 tctaattagc ttttttagtc aatctaaagt tctttattct atgatataat gttactgtag
       61 aaagtttgtg gcggtttggc aaatccaatt gaaggagcgt ggtgcctatg ggcttattag
      121 cttatcggac gtactctgta gaaaggaaat gcctgatttg ttctcattgg atcaaatcat
      181 tgctattatc ggagcagcag cagtagttat tactgccgta gcgaaagcta gcgattcgat
      241 tttagcttgg ttaaagttca aaaaagaaca aaaaaaatag agccgtctca acttttggcg
      301 aggtagacga gctctaaagc tttatttaca tgccatccgc tttgaacgga ctacactggg
      361 catgtgacag catgcccttt catttatatt atatctcatt cttttatata atgaaagtaa
      421 ataaatcatg gaggataaaa tatggataaa tttaatagat taacattaat aaatcagttt
      481 gaaattttaa aagctttaaa tcctaacgaa gaaaaatact atgctgaaaa aatagaaata
      541 ctaacagaag gttacgaata tcactatagt gaaatatttg aaaatatttc tgatccattg
      601 ccagaagaag attcgcggtt tgttctagac atattaaata tgtatagaga tattactttt
      661 agtaaaaata aattaaaaga tataaattca attgataatt attatataca atttaaaggt
      721 tttgacttta atagctctac cgaatttaag ttagcacttt atgcacaatt ttttattgaa
      781 aaattaaata ggtttcaaga aatagttgaa gatagtgatt ttaacacttt taatagccat
      841 aaaccgatga gaggtactta tataaaattt ctcgaaaatt ataatgattt aaaatctacc
      901 aataattatc aatttggaaa cttgtcttat gagatgataa gtaaagttct aagtttgtga
      961 tgataaatga aattaaatag tataaatgat ttatttgcta ttataggtgt tattgctaac
     1021 atactttttt cgtgtttgaa ttttagattt actcaaaaga acaaaaagaa caaactcagt
     1081 atagaacaaa tgaaaaaaga cattgaaact gatttaaaaa atgaccataa agaaagaaga
     1141 aaaattgaag aatatacttc tttaatggaa aagtgtaaag caactgatgc aatttttagc
     1201 tttttaagaa aaagtgatga gataatcaca cgtgaaaata tagatttaga aaatgagatt
     1261 agtaaacttg aatctatctt ttccgatata aattctctta agtatttaat cgtcgaagaa
     1321 aattcggata gctttttaga atttgaaaaa tatctaacag atcttataga taatagaaat
     1381 aatgaattaa tgagacaaaa cattgcaaga ttccttacta aattagtttc tgaatggaaa
     1441 agtattattt ctcaaaatat ggataaatta agatattaag gatgttagtt ttaaaataaa
     1501 ctaatgtcct tatttttgtg ttcacatttc tgttacctaa gaaatactta aaggaggtga
     1561 tgtagccagc aacgactaca acttgaaccc gctatctaca gtttatttgt tgtttctttt
     1621 gggtatactc taatggtaca aatccatatt tgcttaaagc gttgattttt cagcggtttt
     1681 tctttattgc aaagtgtacc ctaagacctg tatactaata gtaaatagat tggataggaa
     1741 tgatggcaaa tgtatttagc ttatttaaga gtttcttccg acgatcaaag tttagctcgg
     1801 caacataagc taattgaaaa ttggtcagtc aaaaataata ttgctgaaga cgatttaaaa
     1861 attttcgagg aaaaagtctc tggaaaaaat attgaaaaca gaccagagct gcaagagctt
     1921 atttctttca tacgtgaacg agatacgatt attattccta gtcttgatcg attagcaaga
     1981 aatagtaaag atacaaaaga cttacttcaa ttgtttcgta gtaaaggtgc ttctattgaa
     2041 atattagacc taccgtcgtt taatggggta actgaccctg cattaagaga cttgctaaca
     2101 aatttagtca tagaagtgtt tagctatgtt gctgaaaacg aacggaaaaa aatcaaagag
     2161 cgtcaaaaac aaggcattga aattgcaaag aaaaatggtc gttatactgg taaaaagatt
     2221 caataccatg ccaatgcaac aggaaaaaac aaattgattt acgatgaaat tgttgatggt
     2281 ctgaagaaaa aagaaactat ttctaaaata gcaaagaacg caggagttac aagaaaaacc
     2341 gtttatcgag taaaaagaga tttaggaatg taatatgttc cagttagaaa gaagggttct
     2401 gttgcaaagt tttaaatcta ctatcaaata aggtagaata atagaaaaag at
//
LOCUS       DACEDM010000143         2408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEDM010000143 DACEDM010000000
VERSION     DACEDM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.153"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            70..1149
                     /gene="uxuA"
                     /locus_tag="IWO50_002828"
     CDS             70..1149
                     /gene="uxuA"
                     /locus_tag="IWO50_002828"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0359889.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            1223..>2408
                     /gene="uxaC"
                     /locus_tag="IWO50_002829"
     CDS             1223..>2408
                     /gene="uxaC"
                     /locus_tag="IWO50_002829"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0359890.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQL"
BASE COUNT          826 a          342 c          509 g          731 t
ORIGIN      
        1 gctagtaggt cctcaagcag ttcgtaaaac aatcaataaa tatataacag aagttggagg
       61 aaagtaacta tggaaatgac ttttagatgg tatggggcaa atgaagaaaa aatctcacta
      121 caagaaattc gtcagatccc agcaatgaaa ggaattgttg gaacattaat ggatatacca
      181 gccggagagg tttggccgag agaacgaatt aaatctttaa aagatgaagt cgaagccaat
      241 ggacttacct tgaaagttat tgagagtgtt aatgttcatg atgatatcaa aattggttta
      301 ccaacacgag atcaatacat tgagaattat aaacagacga ttcgcaattt ggcagaattt
      361 gggattgagg ttatctgtta taattttatg ccagtatttg attgggtaaa atcggatttg
      421 gattatcgtt tagatgatgg gtcttctacg ttagcctata tttcaaaaaa tatccctact
      481 gatccggagg aaattattag aaaagtagaa aattctacta ataattttga attgccgggt
      541 tgggaacctc atcggttatc acaaattaag caattattag aagcctataa agaagttgat
      601 gaagagaaat tacgagagaa ctttgcatat tttctcaaag caattatccc gacatgtgaa
      661 gaagttggga ttaaaatggc agttcaccca gatgatccac catattccat tttcggatta
      721 cctcgtattg ttaaaaatcg tgacgattta gattggttgt gtaatgtagt ggattctcct
      781 tctaatggta ttacattatg tactggaagt attgcagaac gaccagataa tgatgtctat
      841 gaaattctat ctgaattttg tcaacgggat cggattcctt ttgcacatgt tcgtaatatc
      901 aaatatttaa gtgacaatgg actcgatttt tacgaagcgc cgcataaatc agaatatggt
      961 tctctagata tgtataagat attaaaaaca atgtacgata atggatttaa tggctatatt
     1021 cgtccagatc atggtcggat gatttgggga gaaaccggta gacctggtta tggattatat
     1081 gaccgagctc taggtgcagt ttatttaaat ggtatttggg aagcgcttga aaagggggcg
     1141 agtaaataat tatataaggg agtttattta gaggaaaaat tttctcttct aaaaactaac
     1201 ggaaggaaag gagtgtttaa atatgttttt agataagaac tttttattag agaatgaaat
     1261 agctaaaaga ctttttcata aatttgcaaa acaccaacca attatcgact atcattgtca
     1321 tttagaccca gcatatttgc tagaaaataa gcaatacaag aatttgactc aggtatggtt
     1381 aaatgataaa ggagctggag atcattataa atggcgattg atgcgagcta atggtataga
     1441 agaagagttg atcagtggtc caggtgatga ttatgaaaaa tttttagctt ttgtaaaaac
     1501 agtggagaag gcgccaggta atcctatttt tgaatggtct catttggaat tacaacgtta
     1561 ctttgatatt gatttgacaa tttctgagaa gaatgcacca gaaatttggc gacgtgctaa
     1621 cgaaaaaatt caaacggatt cctatcgccc gaaagaattg ttggagatga tggatgtgaa
     1681 agccatctgt acaacggatg atcctacaga tgacttgtgg tatcatactg agctttctaa
     1741 acaggataat tctttcaagg tattacctac tttccgtcca gataaggtct ggagtatcaa
     1801 atcatcaggc ttttcgaact atattttaaa acttagtcaa acagcggcta taccaatcaa
     1861 agactttaat gggttgaagc aagcattgag ccaacgagtg gatcattttc atcaaattgg
     1921 ctgtcgatta gcagatcacg gtttgaatac ttatttttat gaaatagcag atgaaaaaat
     1981 actcgacaat ctgttaataa aagctttagc aggaaaaacg gatttccatt tatcagaaat
     2041 tgcacaattt gttacaggta ttcaattaca tttgatgtct gagtatacaa gatatggttg
     2101 gacgatgcaa atgcatttaa atgctttaag aaataattca agtatattaa aggaacaaat
     2161 tggagtagat gtcggtggtg attcgatggg ggatcaagct agtttgacac aaaatgttgt
     2221 agcattgttc gataccgctg aaaagagtgg gtgcttacca aaaacaattt tatattcatt
     2281 aaatccaact gattggcttc cactagcaac agcaatgcaa agcttccaag ggggtatgat
     2341 tcaaaaaatc caattgggat gtgcttggtg gttcaatgat acagctgaag gaatgcgtca
     2401 acagttga
//
LOCUS       DACEDM010000144         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEDM010000144 DACEDM010000000
VERSION     DACEDM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.154"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWO50_002830"
     CDS             207..2069
                     /locus_tag="IWO50_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0359891.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IWO50_002831"
     CDS             2069..>2400
                     /locus_tag="IWO50_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0359892.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACEDM010000145         2385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEDM010000145 DACEDM010000000
VERSION     DACEDM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.155"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(365..1657)
                     /locus_tag="IWO50_002832"
     CDS             complement(365..1657)
                     /locus_tag="IWO50_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0359893.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="IWO50_002833"
     CDS             complement(1931..2191)
                     /locus_tag="IWO50_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359894.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          805 a          444 c          362 g          774 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
     2341 gtaaaatatt ttgtgtaaat gaaaaaatcc atacaaaaaa ggaag
//
LOCUS       DACEDM010000146         2180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEDM010000146 DACEDM010000000
VERSION     DACEDM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.157"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            700..>2180
                     /gene="ltrA"
                     /locus_tag="IWO50_002834"
     CDS             700..>2180
                     /gene="ltrA"
                     /locus_tag="IWO50_002834"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0359895.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKP"
BASE COUNT          858 a          336 c          455 g          531 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc
//
LOCUS       DACEDM010000147         2143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEDM010000147 DACEDM010000000
VERSION     DACEDM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.158"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(117..707)
                     /locus_tag="IWO50_002835"
     CDS             complement(117..707)
                     /locus_tag="IWO50_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0359896.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1230..1391
                     /locus_tag="IWO50_002836"
     CDS             1230..1391
                     /locus_tag="IWO50_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359897.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1410..1904
                     /locus_tag="IWO50_002837"
     CDS             1410..1904
                     /locus_tag="IWO50_002837"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAQ0359898.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          773 a          332 c          364 g          674 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatccttgt aaagttgagt tttttatttt
     2101 aatgaattaa gactattaaa tagataatta ttgtaactta aaa
//
LOCUS       DACEDM010000148         2104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEDM010000148 DACEDM010000000
VERSION     DACEDM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.159"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWO50_002838"
     CDS             169..1539
                     /locus_tag="IWO50_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAQ0359899.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWO50_002839"
     CDS             1560..1844
                     /locus_tag="IWO50_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359900.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DACEDM010000149         2049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEDM010000149 DACEDM010000000
VERSION     DACEDM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.160"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..110
                     /locus_tag="IWO50_002840"
     CDS             <1..110
                     /locus_tag="IWO50_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359901.1"
                     /translation="IFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            167..559
                     /locus_tag="IWO50_002841"
     CDS             167..559
                     /locus_tag="IWO50_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0359902.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            560..1999
                     /locus_tag="IWO50_002842"
                     /note="aph(2'')-Ia"
     CDS             560..1999
                     /locus_tag="IWO50_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0359903.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          841 a          168 c          350 g          690 t
ORIGIN      
        1 gcatcttccc caatcaaaca tcagccaatc gcttaattgg agccgttctt atggacctac
       61 atgatgaatg gatttattct tcaagaaaat acatcaattt tgataagtag aaatggtaaa
      121 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
      181 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      241 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      301 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      361 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      421 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      481 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      541 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      601 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      661 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      721 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      781 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      841 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      901 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      961 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
     1021 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
     1081 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1141 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1201 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1261 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1321 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1381 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1441 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1501 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1561 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1621 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1681 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1741 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1801 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1861 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1921 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1981 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     2041 taaatagtt
//
LOCUS       DACEDM010000150         2019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEDM010000150 DACEDM010000000
VERSION     DACEDM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.161"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            100..570
                     /locus_tag="IWO50_002843"
     CDS             100..570
                     /locus_tag="IWO50_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0359904.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IWO50_002844"
     CDS             792..1895
                     /locus_tag="IWO50_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0359905.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DACEDM010000151         1908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEDM010000151 DACEDM010000000
VERSION     DACEDM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.163"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(289..362)
                     /locus_tag="IWO50_002845"
     tRNA            complement(289..362)
                     /locus_tag="IWO50_002845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Ile,seq:cat)
     gene            complement(551..1570)
                     /locus_tag="IWO50_002846"
     CDS             complement(551..1570)
                     /locus_tag="IWO50_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0359906.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1581..1787)
                     /locus_tag="IWO50_002847"
     CDS             complement(1581..1787)
                     /locus_tag="IWO50_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0359907.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          562 a          390 c          324 g          632 t
ORIGIN      
        1 atataaaaga gtttaaagcg taacacactt atggggaagt ggcttggggt gcgctttaaa
       61 ctcttcttta ttatcatctc acaatttaac ccaaatgtct ttctatttaa aaatcaaagt
      121 aaaacttttc aaatatacag aaatataact atgtgaaaca tcctttcatt aatccataaa
      181 aggatacata aaaaagccac tcatttgagt ggcaatgaag aaaagcttta gcttgtataa
      241 tactcttcaa aaaattctaa cacagaacga ttcaaatggc tacgttaatg taccctgtag
      301 gactcgaacc tacgaccgga cggttatgag ccgtctgctc tgaccaactg agctaagggt
      361 actggttgtt gccacataaa gccataaaca atcaaccagt agaatgtgtg gcaacaaacc
      421 tgttatcgca tagcttggag tgtgactatt atgggtgata gtgaagatat gcgataacat
      481 cactatttta tcgaatgatt tttatagttg tcaatatagt tatgtaatgc tcctcaacga
      541 ggagctattt ttatcgttta ggaatattta aataccaacg tttgtcatgg aaatcttgcg
      601 caccgccttt agtgtttccc tctggatcat tcgttgcccg catcatgaca tagactttct
      661 tattagggaa gttacgcatg ttaaaagata catgatagcc aacatttcca gaagtattat
      721 aagcttgatt tacatctgat ctataaattc catcagctct tactcgagct aattctttcc
      781 cagtattgta atccataatg aagatatact cgtatttata gttagcaatg tgccatccag
      841 ctacatgcaa atttgcattt tcgatttctc caaactgatc aatgtgggcg taattcgttc
      901 catctgtcag tgtaggatta gctgcaccag ctcgtgttgg atcaatgaca ggcttgtttt
      961 cagaagttgt tggattttca tcggtaaatc catgagctaa atcatatgct aatttttctt
     1021 tacttacgcc catttcagaa agataaccgt aaggatctgt atgatcgccc cagatgtttt
     1081 gtgttaccca taaatgcgat ttgattcccg gttggttata aggagtgtcc aacgttaatg
     1141 gaataccata tttcattgct gaatctctag ccaattcaac gtatgccttg tagtttttct
     1201 caaacgttgc tttatcatgt gtgtgttgta actcaatctg cacaggactg ttggcattag
     1261 catacgaacc agcaccgtac tgtacataac caggttgacc gacttgataa acaattccgc
     1321 cgtctcccac aatataagca gtataagcgc tagtccatga acgttgcata tactgcgctt
     1381 cattgcgtcc tgttgctgtt tcattagccg tttcatgcag taaaatatac tgattatttg
     1441 ctacttgtga gctaccttca tttgcgccca aattaaattc attgttgata gtataggcaa
     1501 atccgttaat tggcaataaa aaaagagccg ttaacaggct catcgcagta atagtaattt
     1561 tctttttcat atctatcctc ctatttatct gatgaaattt tcttatacgt tcgatttgat
     1621 acacctaaaa tactccctag aaatgctcca aaacctgtaa taatcatcac cgttatatct
     1681 gtgtaccgcc agttaacacc ttttccgaca agaccgacaa aagtcgctaa agcggggata
     1741 actactatag cgatccattt caaaatatca aaggttctat tgttcatact atccacctcc
     1801 cttcattggt tgggctactt tctttttcaa tcgcttgttt tcttctatca atatttctct
     1861 ttctgaaagc aaggcatcaa tttgcccgtc cagttgttca attttatc
//
LOCUS       DACEDM010000152         1457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEDM010000152 DACEDM010000000
VERSION     DACEDM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.165"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            109..1041
                     /locus_tag="IWO50_002848"
     CDS             109..1041
                     /locus_tag="IWO50_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0359908.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="IWO50_002849"
     CDS             1113..>1457
                     /locus_tag="IWO50_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359909.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DACEDM010000153         1428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEDM010000153 DACEDM010000000
VERSION     DACEDM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.166"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..242
                     /locus_tag="IWO50_002850"
     CDS             <1..242
                     /locus_tag="IWO50_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0359910.1"
                     /translation="DGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPEV
                     KKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            253..>1428
                     /locus_tag="IWO50_002851"
     CDS             253..>1428
                     /locus_tag="IWO50_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0359911.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     "
BASE COUNT          505 a          272 c          300 g          351 t
ORIGIN      
        1 tggatggtac gtacgatttt gatcgttttt atcaagaaaa aggggatatt aatgctcaga
       61 ttcttttctc ttataaagca gaacaatact acaaaggcta taaaaatcat gaacatgaat
      121 ttgtacctga agtaaaaaag gattggaatg atcaattgaa gcatgaaatc taccaacaag
      181 agatcagaaa gcaaaaacgt gccatgttgt ttcaacaagg tagacagcaa gaaagggagt
      241 gaaatcgtca tgatgaaaac cgttatttta gcagaaaaac cgtcacaagc gaaggcttat
      301 gccgatagct tttctaaagc tacccgaaaa gatgggtatt ttgagatcca agatcgattg
      361 ttttcaggag aaactgtgat cacgtatggt tttggtcatt tagtcgagtt agattcgccc
      421 gatatgtacg atgaaaattg gaaacaatgg tcattagagc atttgcctat tttccctaca
      481 cattatcact atcatgttcc taaagataag aaaaagcaat tcaaggtggt gaaacagcaa
      541 cttcaatcgg ccgacaccat tattattgcg acagatagcg atcgtgaagg ggaactgata
      601 gcttggacga tcattcaaca agcaggcgcc gatcatggaa agatatttaa aagactttgg
      661 atcaactcct tagaaaaaga agccatttat cagggattcc agcagttacg agatgcagaa
      721 gagacctacc caaaatttga agaagcacaa gcacgccaga tcgccgattg gttgatcggc
      781 atgaacggta gcccgctgta tagtttactc ttgcagcaaa aaggcatacc aggaagtttt
      841 tccttaggca gagtccaaac cccaacgctt tacatgattt atcaattaca ggaaaagatt
      901 agaaacttcc aaaaagaacc atattttgaa ggaaaagccc aagtaatcgc tcaaaatggc
      961 gcatttgatg cgaagcttga tccgaatgaa acccaagcga cgcaagaagc atttgaagat
     1021 tatttaaaag aaaagggggt tcaattggga aaacagccag gtacgatcca ccaagttgaa
     1081 accgagaaaa aaagtgcggc aagtccacgt cttttttctc tatccagcct tcaatctaaa
     1141 atgaatcagc tcatgaaagc tagtgccaaa gatactttgg aagccatgca agggttgtat
     1201 gaagggaaat atctaagcta cccacgaaca gatacccctt atattacaga aggggagtat
     1261 gcgtatctgt tggatcactt agacgaatac aaacactttt taaaggccga agccattcct
     1321 actcctattc atacaccgaa tagtcgatat gtgaataata agaaagtcca ggaacattac
     1381 gcgatcattc ctacaaagac ggtcatgacg gcagcggctt ttgaacaa
//
LOCUS       DACEDM010000154         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEDM010000154 DACEDM010000000
VERSION     DACEDM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.167"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWO50_002852"
     CDS             complement(150..1337)
                     /locus_tag="IWO50_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0359912.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEDM010000155         1406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEDM010000155 DACEDM010000000
VERSION     DACEDM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.168"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(96..437)
                     /locus_tag="IWO50_002853"
     CDS             complement(96..437)
                     /locus_tag="IWO50_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0359913.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(1056..1388)
                     /locus_tag="IWO50_002854"
     CDS             complement(1056..1388)
                     /locus_tag="IWO50_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0359914.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
BASE COUNT          407 a          316 c          182 g          501 t
ORIGIN      
        1 aaattcctta ctcaatgcct aaggtaatac taccttttta ttaatcatcg tgactccttc
       61 taagccttta tctgtaaaat ttattcctcc agtttttatt cttctttatc cgctacaaat
      121 tcaatgattt caaagctgac aatataatca ctcaaatatg accaagtatc ttcaacagat
      181 aattcgtctc cattatcatt cataagcgtc attttaaacc attcattacc ttcatcatct
      241 tcaggtagca agccgaatcc aattggatta tcagtcaatg tttgatcgta taaatcatcc
      301 tcgtctaatg ctaaaacatc taatccttta caatttccgc ctcttctaaa cgtcacaaat
      361 cctttgtaat cccaacgttg gaaagaaacc ttaatagtat gtgtgcccaa tcgtatattc
      421 ggatcataat ttttcatttg tcattcctcc attacgtaaa tttcattttc tctatgtttt
      481 atatcaattt tttcacttta ttcaggactt gttcatattt tatcgttttt cgtttactta
      541 aaaatacata aaaaatttcg actcgtttat ggcgtccgaa acgagaaacc ctagtagaca
      601 gcggtaaatg ctcgattgat ccaataatca acgtcctcgc aagtatctac atgtactgca
      661 gcacacgaca caatttcttg tgcgtcctcc ccattacgag aagacctact ccttattcca
      721 tgagtttgcg tcaaagtcta gatcccttag tagctgctca tgattaatct agacgctatt
      781 ctaacttgtc taacttctaa attccctctt gtttactctg cgcttattat ttacacaaat
      841 tcatttcacc actatccaaa caagcgcatt tactcgattg accaattttt tacgttacca
      901 atcgttaaaa cctaccccgt cactccccga agggaattgt tgatagatca agtctactcc
      961 ttatttttct agtgactcta agttccttta gaagtctcga caatattttc aagcaagcga
     1021 aaaacgataa aatacaagtt atgagaatga tcaattcagc cgatcaaacg ccccatccct
     1081 tttgcggtac aacgcaagac tccatttttt cttcgaccaa tcagtacacc gttttggtca
     1141 taaacatgtt tgattgttgg atatagctct tttaagggga taggatcttt taaaattatt
     1201 tgcttttgtt tcccaatgta ataaatcatt tcctctgaga ctttgtcttt ctggattata
     1261 tatttcttac actgcacaac ggttttactt tggccagttg atagtttctt cgtgattttc
     1321 tctgtagcgg tacaccgttt accgtagtga caaatatctt tgagactcaa aattcctgta
     1381 tatgacataa aataaaccac ccaatt
//
LOCUS       DACEDM010000156         1368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEDM010000156 DACEDM010000000
VERSION     DACEDM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.169"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..319)
                     /locus_tag="IWO50_002855"
     CDS             complement(<1..319)
                     /locus_tag="IWO50_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359915.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQN"
     gene            complement(306..1094)
                     /locus_tag="IWO50_002856"
     CDS             complement(306..1094)
                     /locus_tag="IWO50_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0359916.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          394 a          229 c          200 g          545 t
ORIGIN      
        1 tattttgttt ctttgctagt ttatatcctt caatcaagta ttcttcaggt atggatttta
       61 atagcgcatc ttctataacg tctggtttac ttttgtctga ttcagcaata gtcgtccaat
      121 atttgagtgc tgacattaga attggattta tacgaatgct atctgctgtt cttacactac
      181 ttccttgatc ttttatgact ttacttatat ttttcttggt taattcgatt tcttctactt
      241 cttttggttt gtagttcttt ttatttttat ttcctagtag tctacttact gcagcttgac
      301 tgcgattatc ttctgccatc aaaatcacca acttctttta aatattcttc tgtaaatgct
      361 ttatagtcat cttcagctgt tgagggttta ttaaaattga ttagatctga tccagcagct
      421 tgcgcttcgt caacggttat tgttctacga atataagttt tgaaaatttt tgcaccaatg
      481 actctagaaa tttcggttgc tgcagtcgtc atttctttag aaatgttata acgcttatta
      541 tacttagtaa ataggactcc tcgtacttca agcttgtcgt tcgtatattc ttttaccgtt
      601 tcaatagttt cacttaactt tattagtcct tttaatgaga aatacgcagc ttcaactgga
      661 atgataattt catcagaaac agtaaatgcg ttgactgtaa gcattcctaa acttggtgga
      721 caatcaatta taatataatc atagttgtcc ttgattggtt gaagtactct tttaagcctg
      781 tgttcacttc ctacttgagt aaattccctc tctgcagaag aaagggctat gtcggctggc
      841 aaaacatcaa ttcctttata gtccagaata gcatctgtaa tgtcagcctc cccttttaat
      901 acttcataaa ttgtaggttg gtttgttcca tctatatcga aacctacatt atctgtagca
      961 tttccctgag gatcaatgtc cactaaaagt acatttttac cgtaaacaaa tttcaaacat
     1021 gctgctaaat taacgctagt cgtagtttta gaaactccac ctttttgatt ggctactgac
     1081 aatattcttg ccattaaaat atcccccatt catcttatat tataatataa ttatatcata
     1141 atattgtgat attgcaatat cgtatatgca ttatattgta atattgtgtg attattttgt
     1201 tttaaagata tatatcggtt cggatataaa gattattcat atgttattcg aaagtaaaat
     1261 aatcactttt tattacttta aaatttttta ttaatcataa gaaatatgtt ttgcgaggag
     1321 agtgtaaaat attttgtgta aatgaaaaaa tccatacaaa aaaggaag
//
LOCUS       DACEDM010000157         1358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEDM010000157 DACEDM010000000
VERSION     DACEDM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.170"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            23..1324
                     /locus_tag="IWO50_002857"
     CDS             23..1324
                     /locus_tag="IWO50_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0359917.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          440 a          271 c          252 g          395 t
ORIGIN      
        1 cacaatcaaa ggagcgttct gtatgcctga tcttcatcat atcaaactac tacttggaat
       61 caaagataaa aacattttca tcacaaatgt ggaatcgaag agcattaaga atatcaagag
      121 tcttgttgtt tctgcgacgt taagtaaaga aattcgtcgc tgtcctttgt gtaaacagat
      181 gaatcatgaa ggcatgatcg ttaagaacgg aaagaaaaaa tcgctcattc aacttaataa
      241 atgtgcgaac caactgactt atcttgcttt agcgaaacaa cgctaccact gtcgtggctg
      301 ccacacctat tttacggcga atacctacat tgtcgataga aactgcttta tcgctaaaca
      361 agtgcgctat aaaattttag aagagctaac tgaaaaacaa gcaatgacga caattgctaa
      421 gcattgcggc gtttcttggt cgaccgtttc tcggacactc gcttctctga tccccatgac
      481 aaaagtaaaa agaaattggt taccgcgttg tttattaatg gatgaatttc gttcactgaa
      541 aaatcaagtc ggtccctact ccttttcttg tatggatggc gatactggaa aattactgga
      601 tattcttcct tcacggaaga aaaaggctct cgtttcttat tttatgcaat ttgaacgacg
      661 cgcacgattg aaggtaaagg tcatcgtgac ggacatgaat gtttcttatg cctctctgat
      721 aaaggaatgt tttccaaagg caaaactcgt tgtggatcgt tttcacattg tcaaacatct
      781 cattcgtaaa tttgaggata ttcgcgtacg aatcatgaaa agctttgatc gaaacgatcc
      841 aacacaagcc aaatattatc gacaattaaa ggcgttgtct cggcttctga tcaaacgaca
      901 ggacaagctt gtttatgaca aatggaccaa atggcgcaat tttggttggg cgtatctcac
      961 cgaaagtgaa gtggtggatc gtctgctttc gatctctgac gaattacgaa tcgcctattt
     1021 ttactatcaa gaaattcttc aagcgtttca cgacaaagag gcggacactt tctttaaact
     1081 tgtaagaacg atgcccaatt ccgttccaaa agaactgcat tctataaaaa aagcttttat
     1141 aaactatgaa tccggcattc gtttagccct cgaattgcct tattcaaatg ccaaaataga
     1201 aaatctgcat acccacatca aagcattgaa acgagtcgct tatggctttc gaagctttcg
     1261 gaaaatgaag accagaattt tcttactaaa taatctgatt acctatgaat caaaaaacat
     1321 ctaaagtaga aaaggaattc taccttagat gttcttat
//
LOCUS       DACEDM010000158         1356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEDM010000158 DACEDM010000000
VERSION     DACEDM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.171"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>1356
                     /locus_tag="IWO50_002858"
     CDS             82..>1356
                     /locus_tag="IWO50_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0359918.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          461 a          261 c          257 g          377 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaat
//
LOCUS       DACEDM010000159         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEDM010000159 DACEDM010000000
VERSION     DACEDM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.172"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IWO50_002859"
     rRNA            <1..>1355
                     /locus_tag="IWO50_002859"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACEDM010000160         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEDM010000160 DACEDM010000000
VERSION     DACEDM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.174"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(680..753)
                     /locus_tag="IWO50_002860"
     tRNA            complement(680..753)
                     /locus_tag="IWO50_002860"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(717..719),aa:Ile,seq:cat)
     gene            complement(943..>1324)
                     /locus_tag="IWO50_002861"
     CDS             complement(943..>1324)
                     /locus_tag="IWO50_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0359919.1"
                     /translation="AGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIF
                     IMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATND
                     PEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          416 a          244 c          226 g          438 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcatga ctgacgtgcc ggacgcacgt agcgatcatc ggcgttatga
      121 ctatataaag tatactttcg ttgatccaga aaggtataaa gattaaagta ggctacctaa
      181 aaaggtagcc cggaacgaat tttatcacca tacttgtgaa aggagatatt ttttaagtta
      241 gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc tataaaatca
      301 aaaataagtc actaattaac taccactcca attgtaagcc ttttttctca ctttttttca
      361 aaaatatggt atgcttttta atggcttcaa atataaaaga gtttaaagcg taacacactt
      421 atggggaagt ggtttggggt gcgctttaaa ctcttcttta ttattatctc acaatttaac
      481 ccaaatgtct ttctatttaa aaatcaaagt aaaacttttc aaatatacag aagtataact
      541 atgtgaaaca tcctttcatt aatccataaa aggatacata aaaaagccac tcatttgagt
      601 ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaaattcta acacagaacg
      661 attcaaatgg ctacgttaat gtaccctgta ggactcgaac ctacgaccgg acggttatga
      721 gccgtctgct ctgaccaact gagctaaggg tactggttgt tgccacataa agccataaac
      781 aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc atagcttgga gtgtgactat
      841 ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaataa tttttatagt
      901 tgtcaatata gttatgtact gctcctcaac gaggagctat ttttatcgtt taggaatatt
      961 taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc
     1021 attcgttgcc cgcatcatta catagacttt cttactagga aaattacgca tattgaaaga
     1081 tacatgataa ccaacatttc cagaagtatt ataagcttgg tttacatctg gtctataaat
     1141 tccatcagct cttactcgag ctaattcttt cccagtattg tagtccataa tgaaaatata
     1201 ctcgtattta tagttagcaa tgtgccatcc agccacatgc aagtttgcgt tttcgatttc
     1261 cccaaactga tcaatgtggg cgtgatttgt tccatctgtc agcgtaggat tagctgcacc
     1321 agct
//
LOCUS       DACEDM010000161         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEDM010000161 DACEDM010000000
VERSION     DACEDM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.175"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWO50_002862"
     CDS             <1..848
                     /locus_tag="IWO50_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0359920.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaatttta
      961 tttatggtat gacataaata aagtaaagtt attcttctag gttaatctta ttaatactct
     1021 ttctcactag tgttgcatgc aacactagtg agaaagagta ttaaatatac tgcaaggcaa
     1081 gacatttggt aattgaagaa taacaagcaa aaaaatagca gtcaggtctc ttgacaaagt
     1141 atagttgcta ttcttttttt aggccctact ttaatgaaat ttttgtttgg agggaacgac
     1201 ttcattcctt tgattatttc agttgaattt aatagagaag cctggcgtct attaagaaat
     1261 acttttcttg atgagcgtca atttttttaa cggaaatcta tcctatacta aatt
//
LOCUS       DACEDM010000162         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEDM010000162 DACEDM010000000
VERSION     DACEDM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.176"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..1248
                     /locus_tag="IWO50_002863"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IWO50_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACEDM010000163         1302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEDM010000163 DACEDM010000000
VERSION     DACEDM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.177"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..957
                     /gene="uxuA"
                     /locus_tag="IWO50_002864"
     CDS             <1..957
                     /gene="uxuA"
                     /locus_tag="IWO50_002864"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0359921.1"
                     /translation="WPRERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIE
                     NYKQTIRNLAEFGIEVICYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEII
                     RKVENSTNNFELPGWEPHRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGI
                     KMAVHPDDPPYSIFGLPRIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEI
                     LSEFCQRDRIPFAHVRNIKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYI
                     RPDHGRMIWGETGRPGYGLYDRALGAVYLNGIWEALEKGASK"
     gene            1031..>1302
                     /locus_tag="IWO50_002865"
     CDS             1031..>1302
                     /locus_tag="IWO50_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0359922.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFV"
BASE COUNT          439 a          170 c          277 g          416 t
ORIGIN      
        1 tggccgagag aacgaattaa agctttaaaa gatgaagtcg aagccaatgg ccttaccttg
       61 aaagttattg agagtgttaa tgttcatgat gatatcaaaa ttggtttgcc aacacgagat
      121 caatacatcg agaattataa acagacgatt cgcaatttgg cagaatttgg gattgaggtt
      181 atctgttata attttatgcc agtctttgat tgggtaaaat cagatttgga ttatcattta
      241 gatgatgggt cttctacgtt agcctatatt tcaaaaaata ttcctactga tccagaggaa
      301 attattagaa aagtagaaaa ttctactaat aattttgaat tgccgggttg ggaacctcat
      361 cggttatcac aaatcaagca attattagaa gcctataaag aagttgatga agcgaaattg
      421 cgagagaact ttgcatattt tctcaaagca attatcccga catgtgaaga agttgggatt
      481 aaaatggcag ttcatccaga tgatccacca tattctattt tcggattacc tcgtattgtt
      541 aaaaatcgtg acgatttaga atggttgtgt aatgtagtgg attctccttc taatggtatt
      601 acgttatgta ctggaagtat tgcagaacga ccagataatg atgtctatga aattctatct
      661 gaattttgtc aacgggatcg gattcctttt gcacatgttc gtaatatcaa atatttaagt
      721 gataatggac tcgattttta tgaagcgccg cataaatcag aatatggttc tctagatatg
      781 tataagatat taaaaacaat gtacgataat ggatttaatg gctatattcg tccagatcat
      841 ggtcggatga tttggggaga aaccggtaga cctggttatg ggttatatga ccgagctcta
      901 ggtgcagttt atttaaatgg tatttgggaa gcgcttgaaa agggggcgag taaataatta
      961 tataatggag tctatttaga agaaaaattt tctcttctaa aaactaaggg aaggaaagga
     1021 gtgcttaaat atgtttttag ataagaactt tttattagag aatgaaatag ctaaaagact
     1081 ttttcataaa tttgcagaac accaaccaat catcgattat cattgtcatt tagacccagc
     1141 atgtttgcta gaaaataagc aatacaagaa tttgactcag gtatggttaa atgataaagg
     1201 agctggagat cattataaat ggcgattgat gcgagctaat gggatagaag aagagttgat
     1261 cagtggtgca ggtaatgatt atgaaaaatt tttagctttt gt
//
LOCUS       DACEDM010000164         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEDM010000164 DACEDM010000000
VERSION     DACEDM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.178"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWO50_002866"
     CDS             <1..1076
                     /locus_tag="IWO50_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0359923.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tctt
//
LOCUS       DACEDM010000165         1188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEDM010000165 DACEDM010000000
VERSION     DACEDM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.179"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWO50_002867"
     CDS             141..398
                     /locus_tag="IWO50_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359924.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWO50_002868"
     CDS             complement(630..1043)
                     /locus_tag="IWO50_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ0359925.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DACEDM010000166         1141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEDM010000166 DACEDM010000000
VERSION     DACEDM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.180"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(96..947)
                     /locus_tag="IWO50_002869"
     CDS             complement(96..947)
                     /locus_tag="IWO50_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0359926.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT          339 a          243 c          172 g          387 t
ORIGIN      
        1 ttaaaaaata aaaacccaga ttgaaaaaaa cagtcacaaa cattcgtgaa cctatttttt
       61 caatccaggt tttgcctgcc atacagtaac aatccttatg attggatgct ttttttctca
      121 cttactactc tttgaatatg aatcgttaaa taaatctgct ctgcgatccc catttcctgt
      181 ttttgcgttt gtcgaagata ctcattgatc cgttcgacac aatgaaacgc ttttggatac
      241 tttccttgaa ctaatgcgta gagaaattca tcttgctcgt cagatccttc ccctttgaga
      301 taacgctggg caaaaaactg cagatgagta atgatacgct gataattcaa agattcttca
      361 tcgaacacga tgccgaagaa tttactgatg atactcagta tatctcttac cattgtggta
      421 gcggtcatcg tcacttgcat gtcttcgttt ccttgttggc tattcacaaa atgaaaggca
      481 ataaaaccgg cttcatcctc agaaagttcc acacccgtct tttgtttcac gaacaaaaga
      541 gcctctttcg ctgcttgata ttctttcgga taaaattttt ttgtttcaaa taataatgga
      601 ttaggcagtt ggatattttc ttttgctcgt gtgatcgcat aatgaagatg atcggtcaat
      661 gtcagataaa tattaggcga aaattcaact tttagtttat cttcagccag ttcgactact
      721 tgtttgaccc attcgatctc ctctgccgga atatcagcca tcagctgctg gaattggttt
      781 ttagcaaaag aatctcttaa aacaaattct ttttggaccg cttctggcga aatctcgtcc
      841 ccaatctttt tttgaaaagc tagtcctttt cccatacaaa cgacttcttc cccgaatcca
      901 tttttcgaaa tgacgacatt gttattcaaa attttatcaa ttttcatttt gcttcgccct
      961 ctcaagaaaa ataaaaaaac ctagaattcg cctactgttt cttagctgaa ttctggtttt
     1021 gcctgccgta cagtaacaat ccatttctta ttcaaaatca tcataccatc gaatttttat
     1081 ttatgcaagc cttttcatat aaaaaataaa aaaactggaa cacaagtcta tctagaaaac
     1141 a
//
LOCUS       DACEDM010000167         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEDM010000167 DACEDM010000000
VERSION     DACEDM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.181"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWO50_002870"
     CDS             6..980
                     /locus_tag="IWO50_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0359927.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACEDM010000168         1085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEDM010000168 DACEDM010000000
VERSION     DACEDM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.182"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1085
                     /locus_tag="IWO50_002871"
     rRNA            <1..>1085
                     /locus_tag="IWO50_002871"
                     /product="23S ribosomal RNA"
BASE COUNT          332 a          215 c          318 g          220 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctagg
//
LOCUS       DACEDM010000169         1058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEDM010000169 DACEDM010000000
VERSION     DACEDM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.183"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWO50_002872"
     CDS             complement(36..992)
                     /locus_tag="IWO50_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359928.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACEDM010000170         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEDM010000170 DACEDM010000000
VERSION     DACEDM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.184"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWO50_002873"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWO50_002873"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACEDM010000171         1018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEDM010000171 DACEDM010000000
VERSION     DACEDM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.185"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            275..763
                     /locus_tag="IWO50_002874"
     CDS             275..763
                     /locus_tag="IWO50_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0359929.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT          400 a           84 c          168 g          366 t
ORIGIN      
        1 aaattaaaaa tgaaaaaatg gaagcggtta cttattcttt tgaatctaag ataaaaattc
       61 aaaagaataa cataacccaa atatgattat acttgagatt ctaaatattt atcgaaaagg
      121 aatttgtttt aaggaaaaat gattctatgg acggttattg atttatattt accaaatggc
      181 taataaaata gtatttctta tattgtatag tttttgtttt tatgtgaaaa catgagcaat
      241 agaattgtac tagtaggata aggatggatg gaaaatgaaa aaaactattt ttgttttagc
      301 gatttttata tgttttgggc tacttttggt aggctacttg ttgtttaatt ctaatgaaat
      361 gagaactatt tctacgaatg acttgttaaa aaaaagtgaa ttaaaggata aaaaaaaaga
      421 atctcttgcc tttataatgt ttactcaaga agagtgtgag tattgtatag caataaatcc
      481 aatcatagat agtgtaaata aggaaacttt gaataagata aagaaatatg atatatctgt
      541 agattttcaa tctaaacaaa agtgtaaaat taagtttgtt ccgactattg ttgcatatag
      601 taagaataaa gaagtgggga gaattgacaa tgaattagat aatttgtatc gaaaaaagca
      661 agatggttca attgtaatag acaagaaaaa aacaaagaca ttgcttatag attggatgaa
      721 aaaaatggat aaacaaggag aatatttgta tggagaagat taaacagaca tttccgttaa
      781 ttaataaata ttggaataag aaaaaaattt ttatattttt tattacgttt ctgctgtcat
      841 ctttattttc tctaattttc ccgtatctat ttagtaatgt tattgattta tatttagaga
      901 atgagttatc acagaatatt gtgcgaatag taacgattct tatatttcag ttatttttag
      961 tcttaattaa agaagtgtat ggatatataa aaactattta tgcatttgaa gttgattt
//
LOCUS       DACEDM010000172          985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEDM010000172 DACEDM010000000
VERSION     DACEDM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.186"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            50..>985
                     /locus_tag="IWO50_002875"
     CDS             50..>985
                     /locus_tag="IWO50_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0359930.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTK"
BASE COUNT          354 a          180 c          187 g          264 t
ORIGIN      
        1 gctacactca aaatagttcc gacgaaagaa ttataaggag tgagtacaaa tgacctataa
       61 ccatcttaca ccaactgaac tcgtaatgat agaagcttat ttcaatcaat cacaacctgt
      121 ttcaaaagta gccaatctgt tacaacgttc tcgacaaact atttataagg tctatcgttt
      181 tcttaaatct ggtggaagcg caatgagcta ctataaacaa tataagaaaa acaaacgtaa
      241 ctgcggaaga catccgattg ttttacctga tgagcaacag gaatacattc aaaaaaaggt
      301 tgttcaaggg tgggctcctg atgtccttat tggtcgagca gagtttccta ttgcttgttc
      361 ggtacgcaca ctgtatcgaa tgtttaaaga taaaaccttt gacactcacg atttaccaat
      421 gaagggaaaa agaaaaccaa acggtcataa agaaaaaaga ggaaaacaag ggtttcgccg
      481 gtcaatccga gacagaaaga cagactatac ccagtttgat catgaatttg gtcacctgga
      541 aggtgacact atcgtgggtg gaaaacataa aagcgcggtc atcacattag ttgaacggct
      601 atctaaagtc atcattacct tgaaacccga aggcagacag gctaaagaca ttgaaaatag
      661 gctgaatcaa tggtttcgtt gtgtcccaaa aaacttattt aaatccatca catttgattg
      721 tggaaaagaa ttctcaaatt ggaaaaagat tagtaatacc aacgatattt caatttattt
      781 tgcggatcca ggaacacctt ctcaacgagg attgaatgaa cattcaaatg gcttattacg
      841 taaagatggt ttgtataaag ctatggattt taatggtgta tctgaaacct ttatccaatc
      901 ggtggcatcg aaacgaaatc atattcctag aaaatcatta cactatcgga caccaatgga
      961 agtatttttg agttacgtca caaaa
//
LOCUS       DACEDM010000173          977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEDM010000173 DACEDM010000000
VERSION     DACEDM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.187"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            346..977
                     /locus_tag="IWO50_002876"
                     /pseudo
     CDS             346..977
                     /locus_tag="IWO50_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          332 a          180 c          178 g          287 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttac
//
LOCUS       DACEDM010000174          946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEDM010000174 DACEDM010000000
VERSION     DACEDM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.188"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            102..>946
                     /locus_tag="IWO50_002877"
     CDS             102..>946
                     /locus_tag="IWO50_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0359931.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLG"
BASE COUNT          282 a          186 c          184 g          294 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaa
//
LOCUS       DACEDM010000175          917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEDM010000175 DACEDM010000000
VERSION     DACEDM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.189"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            41..>917
                     /locus_tag="IWO50_002878"
     CDS             41..>917
                     /locus_tag="IWO50_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0359932.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LIASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLARE"
BASE COUNT          341 a          168 c          165 g          243 t
ORIGIN      
        1 atgtttttaa cgacaaaaac atacccagga ggacttttac atgacccaag tacattttac
       61 actgaaaagc gaagagattc aaagcattat tgaatattct gtaaaggatg acgtttctaa
      121 aaatatttta acaacggtat ttaatcaact aatggaaaat caacgaacag aatatattca
      181 agcaaaagaa tatgaacgaa cagaaaaccg acaaagtcaa cgaaatggct attatgagcg
      241 cagctttacg acacgtatag gcacgctaga attaaaagta cccagaacac gtgatggcca
      301 tttttcaccc acagtgtttg aacgttatca acgaaacgaa aaagccctca ttgcttcaat
      361 gttggaaatg tatgtatcag gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact
      421 ttgtggtaaa tccgtctcta agtccttcgt ttctagctta acagaacagc tagaaccaat
      481 ggttaacgag tggcagaatc gtttattatc agaaaaaaat tatccttact taatgaccga
      541 tgtactctat ataaaagtac gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc
      601 gattggaata accaaagatg gcgaccgtga aattatcggc ttcatgattc aaagtggcga
      661 aagcgaagag acctggataa cattttttga atacctaaaa gaacgcggtt tacaaggtac
      721 agaactcgtt atttctgatg cgcacaaagg attagtctct gccattagaa aatccttcac
      781 caacgcaagt tggcaaagat gccaagttca cttcctaaga aatatcttta ccaccattcc
      841 taaaaaaaat tcaaaatctt tcagagaagc tgttaaagga atttttaagt tcacagatat
      901 taacttagct cgtgagg
//
LOCUS       DACEDM010000176          902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEDM010000176 DACEDM010000000
VERSION     DACEDM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.190"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            111..893
                     /locus_tag="IWO50_002879"
                     /pseudo
     CDS             111..893
                     /locus_tag="IWO50_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          378 a          127 c          119 g          278 t
ORIGIN      
        1 aagccataaa ggttttcaac aggtaaccga tacattagtt tcaatgttta ctgagtaact
       61 aattattttg caggaggaca atttatttac acaaaattat tgacgctccc caagatatgc
      121 tagattacta ctattttgaa aaaaatttat catataatta tgtaatacat ttaaaaactt
      181 ttacaggaat gatttttaaa ttcgccaacc gtcgatatgg attaaaatat aatcccgtaa
      241 atggggtttc tttagcaaga aaacctaaga caaacgaaga aataaaaaaa gaaaaagatg
      301 gttatttatc aaaagaagaa gttcaaatga ttgctagtaa acaaaaaagt tcacatcaac
      361 agtcacgtta ttctttaatt actaaatttt tgtttttaac tggactacgt tatggtgaat
      421 tgatatcttt gactgaatct gattatgatg gtaataaaat tacggtttct ggtacttatg
      481 attacgagtt aaaaattaaa acaacaacta aaaatacagg ttcttataga accattgaat
      541 tatcaagtaa tgctaaagaa atcatagatc aattaattga agaaaacaaa ttaattaaga
      601 ataataaaga taaatacatt ttcatctcta aaaatggtaa cccgatatct attcaagcat
      661 ataatcaatc tttaagagca gtatccaaag aattaaacat agataaaaaa gtttcttctc
      721 acatgttaag gcattcccac atttctctac ttacagaatt aggaatccca ttaaaagcca
      781 ttatggatcg tgtaggacat gaagattcaa aaacaacatt aaagatttat actcatacaa
      841 ctaaaaatat gcaaaatcaa ctagtagaaa aactaggaaa aatagaaatt taaattgccc
      901 ct
//
LOCUS       DACEDM010000177          892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEDM010000177 DACEDM010000000
VERSION     DACEDM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.191"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            312..608
                     /locus_tag="IWO50_002880"
     CDS             312..608
                     /locus_tag="IWO50_002880"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0359933.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..892)
                     /locus_tag="IWO50_002881"
                     /pseudo
     CDS             complement(531..892)
                     /locus_tag="IWO50_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          286 a          175 c          141 g          290 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga ttaaacaaat caagtattaa gcagatataa
      721 tgccactttt tccccacgcg aacataagta agatccgtaa cgatggcttc caatggctgt
      781 tcttgtgtaa aggtccgatc taatacattc gctgttttcg cttcattaca agctgttcgt
      841 tgccctttaa aatgagcgat cgtataggta gatgtcaatc cgcggcgttt ca
//
LOCUS       DACEDM010000178          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEDM010000178 DACEDM010000000
VERSION     DACEDM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.192"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWO50_002882"
     CDS             <1..888
                     /locus_tag="IWO50_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0359934.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACEDM010000179          856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEDM010000179 DACEDM010000000
VERSION     DACEDM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.193"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(598..741)
                     /locus_tag="IWO50_002883"
     CDS             complement(598..741)
                     /locus_tag="IWO50_002883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359935.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            661..>856
                     /locus_tag="IWO50_002884"
     rRNA            661..>856
                     /locus_tag="IWO50_002884"
                     /product="16S ribosomal RNA"
BASE COUNT          339 a          123 c          159 g          235 t
ORIGIN      
        1 tatatccaac aaagaataag gtacaaaata caagcaagtg agaaattcag ggatgtcatt
       61 ataaaaaaac tagcgaacaa ttaaatagaa taacatccgt taattaaatg taagtatctt
      121 ttagatgaac gaaaaaacaa taaaatgctg taatattcgt gtttaaaaat aataaataaa
      181 aatagggaat tttctattgc aattttataa agagattgct atattattat ttgttctaaa
      241 tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac ttttgatgtc
      301 atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt ttatcgaaaa
      361 aaacttcgaa aaagttattg acaaataaca ataaagatgg tatgataatc aagtcgctgc
      421 taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca ttctcttctg
      481 aatctgataa gattaaagag ttgttaaaac aaaaagatta gacctttgaa aaatgaacaa
      541 agtaagacaa accaaatgtg tagggcgtct tgattcaatt caagacaaca aacattttta
      601 acaagcaagc aatatgctag caaacaaatt gagcttaaca atcgcaagat tgttcgaact
      661 ttttatgaga gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa
      721 gtcgaacgct tctttttcca ccggagcttg ctccaccgga aaaagaggag tggcgaacgg
      781 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta
      841 ataccgtata acaatc
//
LOCUS       DACEDM010000180          854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEDM010000180 DACEDM010000000
VERSION     DACEDM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.194"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(100..300)
                     /locus_tag="IWO50_002885"
     CDS             complement(100..300)
                     /locus_tag="IWO50_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0359936.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          280 a          146 c          131 g          297 t
ORIGIN      
        1 ttttatacac tactattcag cccattcaac gaccaatgaa ttttttcgta caaaaaaacc
       61 gtcttttctt tcgaaaagac ggttaaaagt caatttttat taagctttaa caatgtttgt
      121 tgcttgaggg ccacgttgac catcttcaac atcgtaagac actgcttggc cttcttctaa
      181 agttttgaag ccgtcacctt ggatagctga aaaatgtgca aatacatcat ttccatcttc
      241 accagtgata aagccaaaac ctttgtctgc gttgaaccat tttactgtac cgttattcat
      301 aaataaattc ctcctgatac gtatcaatgt acgttttttc gttgcaataa atatttgata
      361 ggcaaaagga gttttatcta tgtagatata tcgctcaaat tacgtttcaa aatagattac
      421 tcatttaata tatcatgctt tttctaataa cacaaggata atgcaatgaa atataaaatt
      481 cattgtcttc tattgtcttc gaatcgaaga aaaagttgaa taaccgcgat tgattcactt
      541 tttgttttct tgtttatttg cttttatctg caacttttga ctttaaaatt tcttaactat
      601 gaaaaccttt caattggtgg tatacttata aatggaattt cgttatcaac gcgttcgttg
      661 cctttcagca agtatttaaa gaaaggagaa tggctatgac attctataac aaagatatac
      721 aatatcgagt aaccttggat acagatttga atctatttgt cgtttttgac aaagaaaata
      781 ccaatcatgt agcgacaggt gttacgatcg aacaagcagt gcaagaatta aaaaaatctg
      841 cataaggagt ggaa
//
LOCUS       DACEDM010000181          775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEDM010000181 DACEDM010000000
VERSION     DACEDM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.195"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(249..773)
                     /locus_tag="IWO50_002886"
     CDS             complement(249..773)
                     /locus_tag="IWO50_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359937.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          320 t
ORIGIN      
        1 tgacgctccc ttaacaataa atgaagactt ttttattgtt aattgtttat ttttctatta
       61 ttagatatga aaatcgcatt tagataaaat agaaaaatat gaatatattt taaatattaa
      121 gatgagagtc attaacttat gatagtagtc aatttgcttt tcactaatta caaaatatgt
      181 gatataggaa gtttagttat tgttctcata atgccatcct gtttttagat tatctagtaa
      241 tttttttgtt atttaaaatt aggagcagtg ccaattgttt taattttaaa ggcttttttt
      301 atatcttcta aattagaaga tatttgtata tcaatatgga atatattacc ttttttgtat
      361 ttttttatgt tatctttagc taaaacatca aatacatgtc cttgaaattg tatcgaggca
      421 gctggattta gtttaaattg aggaaacttt accacagctg attcaaaaca cttgtcacat
      481 ttttttattt ttttaccttt gtaaaaatat tcgattgaat attctatttg gcacgactca
      541 cttggcagta tatgtatgtc aatatttttc atctgtttca taggtatttc aaaatctaaa
      601 ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa atttatcttt
      661 ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc tctatcaaaa
      721 tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat caaac
//
LOCUS       DACEDM010000182          755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEDM010000182 DACEDM010000000
VERSION     DACEDM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.196"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..242
                     /locus_tag="IWO50_002887"
     CDS             <1..242
                     /locus_tag="IWO50_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0359938.1"
                     /translation="NGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPEV
                     KKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            253..>755
                     /locus_tag="IWO50_002888"
     CDS             253..>755
                     /locus_tag="IWO50_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0359939.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQAR"
BASE COUNT          270 a          137 c          161 g          187 t
ORIGIN      
        1 cgaatggtac gtacgatttt gatcgttttt atcaaggaaa aggggatatt aatgctcaga
       61 ttcttttctc ttataaagca gaacaatact acaaaggcta taaaaagcat gaacatgaat
      121 ttgtacctga agtgaaaaag gattggaacg atcaattgaa gcatgagatc cagcaacaag
      181 aaattagaaa gcaaaaacgt gcgatgttgt ttcaacaagg cagacaacaa gaaagggagt
      241 gagatcgtca cgatgaaaac tgtcatttta gcagaaaaac cgtcacaagc gaaggcttat
      301 gccgatagct tttctaaagc cacccgaaaa gacgggtatt ttgagatcca agatcgattg
      361 tttcctggtg aaactgtgat tacgtatggt tttggtcatt tagtcgagtt agattcgccc
      421 gatatgtacg atgaaaattg gaaacaatgg tcattagaac atttacctat tttccctaca
      481 cattatcact atcatgttcc taaagataag aaaaagcaat tcaacgtggt gaaacagcaa
      541 cttcaatcgg ccgacaccat tattattgcg acagatagcg atcgcgaagg ggaactgatc
      601 gcttggacga tcattcaaca agcaggcgcc gatcatggaa agatatttaa aagactttgg
      661 atcaactcct tagaaaaaga agccatttat cagggattcc agcagttacg agatgcagaa
      721 gagacctacc caaaatttga agaagcacaa gcacg
//
LOCUS       DACEDM010000183          754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEDM010000183 DACEDM010000000
VERSION     DACEDM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.197"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            363..731
                     /locus_tag="IWO50_002889"
     CDS             363..731
                     /locus_tag="IWO50_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0359940.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          244 a          118 c          157 g          235 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttc
//
LOCUS       DACEDM010000184          734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEDM010000184 DACEDM010000000
VERSION     DACEDM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.198"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            100..630
                     /locus_tag="IWO50_002890"
     CDS             100..630
                     /locus_tag="IWO50_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359941.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(625..>734)
                     /locus_tag="IWO50_002891"
     CDS             complement(625..>734)
                     /locus_tag="IWO50_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0359942.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          295 a           86 c          111 g          242 t
ORIGIN      
        1 aatttctaac atcagttatt atgataattt tgatgatgtc aattatgatt tacaggaaag
       61 atataatgat ttaatcgaag aattagagga gagaggagaa tgatttcaga taataaatgg
      121 gaaataaatc tcaaaagatt tatttcagaa tatatatata cgattgaaac taaatctagt
      181 ttttctctta atgaagacag tgatcctcta aaaaaataca ctattcaatg taagaaaaat
      241 aaagatgaaa aattgttcgt aataagacca gatcttatga agagtaatac ccactatata
      301 aaagaaaaac ctaaagattg tgactacatc atcataagtg aagcaaataa aacagtattt
      361 ctgatagaac ttaaaagctc agctcagact tcccctgcaa aagaaattag tgaacaaatt
      421 gatggtggat ataagtggtt acaacatctt agttttctaa caaaatatga ttttactgaa
      481 ttcagttata aaattattaa ggtatgttgt aaatttgagg caagacaaga tcgtaaaaat
      541 ctttttaaac ctaatgaata tggtatttat aatatgaatg gtccaaaatt agacttaaga
      601 gagttgtata gaaagtgtaa cgatctataa attcaaatct tttttaattc tatatattgt
      661 ttgtctagta acgttataat tctttgcaat ctttgaaata gagacgccct cattcaagtc
      721 cttaacgatg tttt
//
LOCUS       DACEDM010000185          722 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEDM010000185 DACEDM010000000
VERSION     DACEDM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.199"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>722
                     /locus_tag="IWO50_002892"
     CDS             <1..>722
                     /locus_tag="IWO50_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAQ0359943.1"
                     /translation="YYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKRGIQ
                     ISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLT
                     YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRG
                     KEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDL
                     ELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          259 a          125 c          136 g          202 t
ORIGIN      
        1 ctattattat caatcaaaac caaagaaaga cgaatcagaa cttgaagaag tagtcgctga
       61 agaatttatc cgcagccgaa aggcctacgg ctcaagaaaa ataaaaaaag ccttatcaaa
      121 acgaggcatt cagatcagcc gacgaaaaat tagtagaatc atgaaaaata gaggattaaa
      181 atcgagctat actgttgctt attttaaagt acatcattct acttgcaatg aagccaaaac
      241 gacaaacgta ttgaatcgta aattcttaag agacaaccca ttagaagcga tcgtaacaga
      301 cttgacttat gtacgagtcg ggaaaaaatg gaattatgtc tgtttcattt tggatctgtt
      361 caatcgagaa attctcggct attcttgtgg agaacataaa gatgccgttc tagtaaaaaa
      421 agcatttagc cgtatcaaac aacctctgac agaggttgag atttttcata ctgatcgtgg
      481 aaaagagttt gataaccaag ctattgatga attattaaca acttttgaca tcaatcgatc
      541 attgagtcat aaaggctgtc cttttgataa tgccgtagct gaatcaactt ataagtcgtt
      601 gaaagtagaa tttgtctatc aatacacatt tgaaacctta caacaattgg atttggagtt
      661 atttgactat gtcaattggt ggaaccacct tcggttgcac ggtacacttg gctacgagac
      721 ac
//
LOCUS       DACEDM010000186          713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEDM010000186 DACEDM010000000
VERSION     DACEDM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.200"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..576
                     /locus_tag="IWO50_002893"
                     /pseudo
     CDS             1..576
                     /locus_tag="IWO50_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          301 a           76 c          114 g          222 t
ORIGIN      
        1 aatattgagc tgttaatgga gacaacggat aatagttctt atccaaagcc aatatcggat
       61 tatattaatt atctgaataa catgaaaaat agatatgaac agaaaaatgt caataatata
      121 gcatcatata ttagagatga gattattatg ggtaaatatg aaattgacga aaaagataat
      181 tctgtaagtt ttagacaaaa gtttggtaaa aatagatata gaaaagaacg aattcctttt
      241 cacgttgtgt cttcttcgat taaatctcta tatggattag attattactt agaaaattca
      301 agtaaagaag gagactatct tattattgat gagccagaat tgagtttaca tccagaaaat
      361 caggttaagt tagcaaaagt aattacagat atagttgaga gtaaaattaa gctaattact
      421 tctacccata gtgatttatt tgtacgtgag cttacaaata ttgttattga aaataaatta
      481 aatagtaaga ataataaaaa aaataaattg tctgataaaa atataggcat atataatttt
      541 gtaggaaata atgatataaa tgaattagaa aaaatttcga taaagtccgt ataattgtgt
      601 aaaagtaaaa aggccatata acagtccttt tacggtacaa tgtttttaac gacaaaaaca
      661 tacccaggag gacttttaca tgacccaagt acattttaca ctgaaaagcg aag
//
LOCUS       DACEDM010000187          665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEDM010000187 DACEDM010000000
VERSION     DACEDM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.201"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            16..>665
                     /locus_tag="IWO50_002894"
     CDS             16..>665
                     /locus_tag="IWO50_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAQ0359944.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLAL"
BASE COUNT          235 a          119 c          112 g          199 t
ORIGIN      
        1 aaatgaggac tacatatgcg ttcatcaaaa tatactgaac attatacaga tacttttata
       61 acattcaagg aaattatgag aactgtttct aacatatacc acaattgtgt accagataag
      121 attaaaaacc gaagaaatac tgatcaactg aagcaacgcg atacagtgat tattgcttgt
      181 gttatatggg gtataattaa tggctatact agccaaagag ccacgtatag agcggtttgt
      241 tccgtcttat ttcctaatgg tgactttcct agtaggagtc gattcactcg cttaagttca
      301 aacttagctt acactatcaa gattattcga tactttttca tcaagaagct caccaaaggt
      361 gaactagtcg gaattataga tagttttcct agtccattat gtaaaccagt tagaaataga
      421 caagcaaaac tattgaatca aatagccaaa gttggctata actcaacaaa aaaatcttat
      481 ttttatggtc ttaaaattca tatgatcgtt actaagactg gctttccgat tacttactca
      541 atcacgaatc caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat
      601 cttcccaata tcctagggga caaaggatat attagccata aaatccatga aaagcttgcc
      661 cttaa
//
LOCUS       DACEDM010000188          601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEDM010000188 DACEDM010000000
VERSION     DACEDM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.202"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>601
                     /locus_tag="IWO50_002895"
     CDS             <1..>601
                     /locus_tag="IWO50_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0359945.1"
                     /translation="YEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGW
                     KKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAG
                     KTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVE
                     KEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKFVS"
BASE COUNT          242 a          102 c          138 g          119 t
ORIGIN      
        1 tttatgaaca agtgttaaaa acaacggtgg ccatgtttgc ggaaaagtat acctatgaag
       61 aaacaacgat cttgacgcaa gttcagcagc ttcagctaaa gacagtcgga aaagtaccag
      121 tagatctagg ttggaaaaag ctgtttggca aagagagcga cagaaaagaa aaggaagaag
      181 aaccgctact tcctaaagtg acaaaaggag aagcggtgac ggtagatcta caagtccttg
      241 aaaaagaaac gaagccacca caaccttata ccgaaggtac gttgatcaca gcgatgaaaa
      301 cagctggaaa aaccgtggat agcgaagaag cacaatccat attgaaagaa gtagaaggaa
      361 tcggtacaga agcaacacga gcaaatatta ttgaaacttt gaaacaaaag gaatatatca
      421 aggtagaaaa aaataagctt gtggtgacaa ataaagggat tttactttgc caagccgtgg
      481 aaaaagagcc gctgctaaca agtgccgaaa tgaccgccaa atgggaaagt tacttattaa
      541 agattggtga gcagaaagga acacaagcta ctttcttagc taatatccaa aaatttgtct
      601 c
//
LOCUS       DACEDM010000189          588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEDM010000189 DACEDM010000000
VERSION     DACEDM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.203"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(65..150)
                     /locus_tag="IWO50_002896"
     tRNA            complement(65..150)
                     /locus_tag="IWO50_002896"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="IWO50_002897"
     tRNA            complement(167..238)
                     /locus_tag="IWO50_002897"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="IWO50_002898"
     tRNA            complement(250..322)
                     /locus_tag="IWO50_002898"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="IWO50_002899"
     tRNA            complement(340..421)
                     /locus_tag="IWO50_002899"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="IWO50_002900"
     tRNA            complement(429..501)
                     /locus_tag="IWO50_002900"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="IWO50_002901"
     tRNA            complement(505..577)
                     /locus_tag="IWO50_002901"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACEDM010000190          577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEDM010000190 DACEDM010000000
VERSION     DACEDM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.204"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            96..566
                     /locus_tag="IWO50_002902"
     CDS             96..566
                     /locus_tag="IWO50_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0359946.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          209 a          102 c           89 g          177 t
ORIGIN      
        1 tttattgtct gaaaagagtt ctaaacggga aaacaaatag ctagttttca gtttagcgct
       61 ctttttatta tataaaatag aaaggagaaa actttatgtc tatttataat tttgaaacta
      121 ctttagaaga cggcactgtc tattcattga aaaaatatgc aggcaaaccg ataatcattg
      181 ttaacactgc aacaaaatgc ggtctagcac ctcaatttaa acaattagaa gaactttacc
      241 aagcttatca gacacaaggt ctggttatct taggctttcc ttccaatcaa tttaaacaag
      301 agctagattc agcgactgaa gcagcagaag cttgtaaaac cacttatggc gttaccttcc
      361 ctatgcatca attgattgac gtaaatggag aaaatacaga tcctcttttt acttatctta
      421 aaaatgaagc tcctggtact gtcactaaag caataaaatg gaattttact aaatttttga
      481 ttgatagaaa tggcaaagta atcgaacgat ttgcaccaca aacttctcca ttgaaaatga
      541 ccgctgcaat tgaatccgtt ctttaactta ataaaaa
//
LOCUS       DACEDM010000191          529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEDM010000191 DACEDM010000000
VERSION     DACEDM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.205"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            33..>529
                     /locus_tag="IWO50_002903"
     CDS             33..>529
                     /locus_tag="IWO50_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0359947.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          194 a           98 c           87 g          150 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DACEDM010000192          527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEDM010000192 DACEDM010000000
VERSION     DACEDM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.206"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(60..281)
                     /locus_tag="IWO50_002904"
     CDS             complement(60..281)
                     /locus_tag="IWO50_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0359948.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          184 a           88 c          102 g          153 t
ORIGIN      
        1 tgttttcatt aagacgtatt acctaagagt tccatgactt tagtctggta gcgtgtcagc
       61 taaccaaaaa agagaaaaag aaaagtaaaa aattcgactt tcttttttct atagtatata
      121 aatttttgca tatagataaa gatcaagata ccgataacgg aagctagatt gtaatctatt
      181 ttagtgacag tcaggtatag ccaagtaact agaagatcag tggaacctgc accataaaag
      241 tttgagtttg tcggtccgtt gacagttaaa agaaaaatca cgttgaagtt attgatattt
      301 ccgattacgc cgcttcgatt tggtcacgat ccaaattttg gataatccct gtagaaatta
      361 gcatagtagc tgggatacca attcacatat tgacaaagat aacagagact tttacataaa
      421 acggatcaat caggaaagga attgctcgat ccagccccag ttttccaaca atgtgttaac
      481 tggcctagcg tcattcagca aattatgcat gatgaggagc gaaatga
//
LOCUS       DACEDM010000193          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEDM010000193 DACEDM010000000
VERSION     DACEDM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.207"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWO50_002905"
     CDS             complement(<1..132)
                     /locus_tag="IWO50_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0359949.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACEDM010000194          511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEDM010000194 DACEDM010000000
VERSION     DACEDM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.208"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(38..127)
                     /locus_tag="IWO50_002906"
     tRNA            complement(38..127)
                     /locus_tag="IWO50_002906"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Ser,seq:tga)
     gene            complement(151..224)
                     /locus_tag="IWO50_002907"
     tRNA            complement(151..224)
                     /locus_tag="IWO50_002907"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Ile,seq:cat)
     gene            complement(238..311)
                     /locus_tag="IWO50_002908"
     tRNA            complement(238..311)
                     /locus_tag="IWO50_002908"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(275..277),aa:Met,seq:cat)
     gene            complement(339..411)
                     /locus_tag="IWO50_002909"
     tRNA            complement(339..411)
                     /locus_tag="IWO50_002909"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(376..378),aa:Ala,seq:tgc)
     gene            complement(431..504)
                     /locus_tag="IWO50_002910"
     tRNA            complement(431..504)
                     /locus_tag="IWO50_002910"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(468..470),aa:Pro,seq:tgg)
BASE COUNT          136 a          145 c          119 g          111 t
ORIGIN      
        1 agctaccaga ctgctccatc ccgcgataat atgaaaaagg aggataaggg attcgaaccc
       61 ttgcacggtt ttacccgcct gacggttttc aagaccgttc ccttcagccg gacttgggta
      121 atcctccata tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg
      181 gacggttatg agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg
      241 cggcggaggg gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga
      301 gctacaccgc gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc
      361 gctgacctcc tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa
      421 tttataatta tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc
      481 tctaccaagc tgagctactt cccgtttaat t
//
LOCUS       DACEDM010000195          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEDM010000195 DACEDM010000000
VERSION     DACEDM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.209"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACEDM010000196          496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEDM010000196 DACEDM010000000
VERSION     DACEDM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.210"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..387
                     /locus_tag="IWO50_002911"
     CDS             <1..387
                     /locus_tag="IWO50_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0359950.1"
                     /translation="RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGK
                     CAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIAL
                     LKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          193 a           78 c           89 g          136 t
ORIGIN      
        1 agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca gttaatgaaa
       61 ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct attttctgct
      121 caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga aatacattgt
      181 catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct tattttggta
      241 aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca aaaatatata
      301 gctctattga agctaaataa agtccagctc ataagactaa ataaatatag agtattagct
      361 ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca atataaacac
      421 gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg aaaagtttga
      481 gatatttata atatca
//
LOCUS       DACEDM010000197          481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEDM010000197 DACEDM010000000
VERSION     DACEDM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.211"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(58..255)
                     /locus_tag="IWO50_002912"
     CDS             complement(58..255)
                     /locus_tag="IWO50_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0359951.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          156 a           89 c           67 g          169 t
ORIGIN      
        1 tttttctacc gacataaaca aaaaaaccac ctcatatgag gcggttaacg ttcaacatta
       61 ttttacgata tttactgctt gaggaccacg ttgtccttct tcaatatcat aagtaactgc
      121 ttggccttca tctaaagact taaagccttc tccttggatt gctgagaaat gagcgaatac
      181 gtcatttcca tcttcaccag tgatgaaacc aaaaccttta tctgagttaa accattttac
      241 tgtaccgtta ttcatatata tttcctcctg atacgtatat aatacgtgtt ttattgcaat
      301 taatattact tggtaaaagg agtttttata gtgtgaatat atcgctcaga ttacgtttca
      361 aattagatta cttatttact ataacacgga tgtaatgcta acacaaggat aatcactcga
      421 tatacggttt aaatattatt atttgtcaaa ttaagcgaga caaaactttc catttacgta
      481 a
//
LOCUS       DACEDM010000198          480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEDM010000198 DACEDM010000000
VERSION     DACEDM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.212"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>480
                     /locus_tag="IWO50_002913"
     CDS             <1..>480
                     /locus_tag="IWO50_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0359952.1"
                     /translation="HFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTT
                     IYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYL
                     "
BASE COUNT          166 a           80 c           95 g          139 t
ORIGIN      
        1 cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg ctactatctt
       61 cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt
      121 tctcatacga cgatttatcg ttgggtgcaa gaatatggca aactactcta tcaaatttgg
      181 aaaaagaaaa ataaaaaatc cttttattca tggaaaatgg atgaaacgta catcaaaatt
      241 aaaggaaaat ggcattattt gtatcgagcc atcgatgcag atggtttaac cttggatatt
      301 tggttacgta aaaaacggga cacacaagca gcctatgctt ttcttaagcg gttagtgaag
      361 cagtttgatg aaccgaaggt tgtagtcaca gataaagccc cctctattac aagtgccttt
      421 aagaaactaa aagaatacgg cttttatcaa gggacagaac atcgtaccat taaatacctg
//
LOCUS       DACEDM010000199          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEDM010000199 DACEDM010000000
VERSION     DACEDM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.213"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IWO50_002914"
     CDS             complement(<1..430)
                     /locus_tag="IWO50_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0359953.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACEDM010000200          461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEDM010000200 DACEDM010000000
VERSION     DACEDM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.214"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>461
                     /locus_tag="IWO50_002915"
     CDS             <1..>461
                     /locus_tag="IWO50_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0359954.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKV"
BASE COUNT          184 a           74 c          106 g           97 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tttgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtaa ccaacctacc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct ggtggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt c
//
LOCUS       DACEDM010000201          440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEDM010000201 DACEDM010000000
VERSION     DACEDM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.215"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>440
                     /locus_tag="IWO50_002916"
     CDS             <1..>440
                     /locus_tag="IWO50_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0359955.1"
                     /translation="FEEKIPHFSTFGKNYVRQFRETTVFEDIFAYILEQAVKAGFVTE
                     DNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKE
                     VKLKERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILST"
BASE COUNT          160 a           78 c           91 g          111 t
ORIGIN      
        1 ccttcgagga gaaaatcccc catttctcaa cttttggaaa gaattatgtt cgccaattta
       61 gagaaacgac ggtgtttgag gatatttttg cctatatttt agaacaagca gttaaagcag
      121 gttttgtaac agaggataat ttgtatctgg attctaccca catcaaagct aacgccaata
      181 aacataagtt tacgaaagaa atgacccatg atgaagcgaa agcctatcag gatgaattag
      241 aagatgagat caatcgtcaa cgaatagagg ctggaaaacg cccttttact ttggacttag
      301 aaaaggaagt gaagctgaaa gaaagaaaaa taagtaaagc tgatccggaa agcggctact
      361 acgtcaaagg cgaacgagaa aaacaatttg cttattctgc ccatacttca tgcgacgaca
      421 atggatttat tcttagcaca
//
LOCUS       DACEDM010000202          413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEDM010000202 DACEDM010000000
VERSION     DACEDM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.216"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            76..>413
                     /locus_tag="IWO50_002917"
     CDS             76..>413
                     /locus_tag="IWO50_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0359956.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRF"
BASE COUNT          148 a           83 c           77 g          105 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt tcctgctgag
      301 gaaaaagaat acattgaaaa aagatcaact gaaggttgga ccccagacgt cattattggc
      361 cgtgcagagc gaaccttctc ttgttcagta agtaccctct atcgccggtt taa
//
LOCUS       DACEDM010000203          387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEDM010000203 DACEDM010000000
VERSION     DACEDM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.217"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>387
                     /locus_tag="IWO50_002918"
     CDS             <1..>387
                     /locus_tag="IWO50_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0359957.1"
                     /translation="EEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQ
                     DILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLEC
                     LNNHIKVLKRNAYGFRNFYNFKLRIM"
BASE COUNT          134 a           78 c           71 g          104 t
ORIGIN      
        1 aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt
       61 tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct
      121 atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg
      181 attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt
      241 ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat
      301 tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact
      361 tttacaattt caaactacga attatga
//
LOCUS       DACEDM010000204          374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEDM010000204 DACEDM010000000
VERSION     DACEDM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.denovo.218"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>374
                     /locus_tag="IWO50_002919"
     CDS             82..>374
                     /locus_tag="IWO50_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0359958.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRT"
BASE COUNT          133 a           62 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa ccca
//
LOCUS       DACEDM010000205        13426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.081, whole genome shotgun sequence.
ACCESSION   DACEDM010000205 DACEDM010000000
VERSION     DACEDM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.081"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..350
                     /locus_tag="IWO50_002920"
     CDS             <1..350
                     /locus_tag="IWO50_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0359959.1"
                     /translation="MVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKVPYGV
                     AVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKIL
                     GQTLSRFFTILPK"
     gene            608..2110
                     /locus_tag="IWO50_002921"
     CDS             608..2110
                     /locus_tag="IWO50_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0359960.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2177..2704
                     /locus_tag="IWO50_002922"
     CDS             2177..2704
                     /locus_tag="IWO50_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0359961.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2915..3976
                     /locus_tag="IWO50_002923"
     CDS             2915..3976
                     /locus_tag="IWO50_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0359962.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3979..4875
                     /gene="murQ"
                     /locus_tag="IWO50_002924"
     CDS             3979..4875
                     /gene="murQ"
                     /locus_tag="IWO50_002924"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0359963.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4889..6871
                     /locus_tag="IWO50_002925"
     CDS             4889..6871
                     /locus_tag="IWO50_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0359964.1"
                     /translation="MAKNELTLEELAKHIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6936..7382)
                     /gene="nrdI"
                     /locus_tag="IWO50_002926"
     CDS             complement(6936..7382)
                     /gene="nrdI"
                     /locus_tag="IWO50_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0359965.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7593..9017
                     /locus_tag="IWO50_002927"
     CDS             7593..9017
                     /locus_tag="IWO50_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0359966.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(9198..9971)
                     /locus_tag="IWO50_002928"
     CDS             complement(9198..9971)
                     /locus_tag="IWO50_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0359967.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(9968..10873)
                     /locus_tag="IWO50_002929"
     CDS             complement(9968..10873)
                     /locus_tag="IWO50_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0359968.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11027..11347)
                     /locus_tag="IWO50_002930"
     CDS             complement(11027..11347)
                     /locus_tag="IWO50_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0359969.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            11821..12507
                     /locus_tag="IWO50_002931"
     CDS             11821..12507
                     /locus_tag="IWO50_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0359970.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12509..>13426
                     /locus_tag="IWO50_002932"
     CDS             12509..>13426
                     /locus_tag="IWO50_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0359971.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQ"
BASE COUNT         4249 a         2359 c         2912 g         3906 t
ORIGIN      
        1 agatggtttc tggtttcgat ttagtcagcc aaatcactgg attggaaaaa aagatcgaac
       61 aagcagatgt catcattaca ggagaagggc ggatcgatca gcaaacgatc cacggaaaag
      121 ttccatacgg tgtagcagtg cttgcaaaaa aatatcgaaa accaataatc gctatttgcg
      181 gaagtagaga ccaagagcta gaaggacttt ctgattttct cccaattgtc ttaagtattc
      241 atttgggtcc aataggttta gaagaagcaa tggaacacag gcaaacatta gaaaaaacaa
      301 aaatcttagg acagacactt agcagatttt tcaccatcct cccgaagtag ttgacaaaat
      361 cattttcttt atgctatcgt aatttcaaat ataggacaaa gtgagggatc cgtgcattga
      421 aaaattaagt cgattgatga aagctgcatt cagactgtac cttgtggaga caaggaagca
      481 gtgtgcagat tttcgggatt ggcttggttt tttcgatgta tgggtctttt tgtgtgtatc
      541 caacccttgc atcctcttta gccagttctg aaaataggac tggtcttttt ttacgaggtg
      601 atggaaaatg tctaaaatcg aaataaaaaa tctgacattc ggctacgaca gccaaggcac
      661 attattattt gaacaagcaa atctaaattt tgacacacaa tggaaactag gacttatcgg
      721 acgaaacggt cgaggaaaga caactttact gaatattcta caaaacaaac taccttatca
      781 agggcaagta atccatcagc aagaatttgc ctatttcccg caacagacaa aagataaaga
      841 acgtttaacc tattacgtgt taaatgatat tacggatttt gagatatggg aaatcgaaag
      901 agagctccaa ttgatgcaaa cagatcctga aatcttatgg agagaattca gcacactatc
      961 ggggggagag aagacaaaag tcctactggc acttttattt gtggatgaca ctcatttccc
     1021 gttaatcgat gaaccaacga atcatttgga tatctctggt agaaaacaag tagcggctta
     1081 tttgaaaaag aaaaaacaag gcttcatcgt ggtcagccat gaccggggat ttatcgatga
     1141 agtagtggac catgttttag caatcgaaaa aagtcaactg gaactttatc aagggaattt
     1201 ctctatctat gaagaacaga aaaaacttcg tgatgaattt gaaatggctc aaaatgaaaa
     1261 attgaaaaaa gaagtcagta ggctaaagaa aacagcagct gaaaaagccg aatggtctcg
     1321 ttcccgagaa ggagataaaa caaagaaaca agtcggattc atcgatactg aatctagacg
     1381 agtgaataaa ggagcagtgg gtgctgatgc tgcacggacg atgaaacgat ccaaagcaat
     1441 cgtgaatcgg atggagaccc agatcagcga gaaagaaaaa ctattaaaag atatcgaata
     1501 tatcgattct ttgacgatga atagccaagc gtctcaccat aagcgacttt taagcgtaga
     1561 agatcttcaa ttagggtatg aaaatctgtt attcgagcca attcatttta caatcgagcc
     1621 tcatcagcgg gtggcgattt caggtcctaa cggtgcagga aagtcatcca ttatccatta
     1681 tcttctgggg gcattcaacg gcaaggttat aggagaaaaa taccagccaa aacatctgag
     1741 cattagttat gcaagccaaa attatgaaga caatcgagga acgttggcgg aatttgcaga
     1801 gaaaaaccaa gtagactacc aagcattttt gaacaacctc cgaaagcttg ggatggaaag
     1861 agatgttttt cataacaaga tcgagcagat gagtatgggc caacggaaaa aagtggaatt
     1921 ggctaaatct ttatcacagc cagctgaact atatacatgg gatgaaccat tgaattattt
     1981 ggatgtcttc aatcaagaac aattagaaca actgatcttg aacgtgaaac ctgccatgtt
     2041 actagtggaa catgatcaaa ccttcctgga taaagtatct actgagatta tttctcttga
     2101 gagaatctaa cccttgtaat ctcaagaaca acaaggtatg gttaagggga actttgccaa
     2161 cgaaggagag aatcacatgc tttatgtaac gagacacgga gaaacaactt ggaacgcaca
     2221 aggattagtt tgcggacggg cagatgtgcc gctgactgaa aaaggacaga tgcaggcaca
     2281 gaaactagca gaaaaggtag tagatttgcc tgttccaatc acgaaaatca tccattcccc
     2341 tcttcaacga gcaagagata cagcacaagc agttgctgat cgtctcagct tgccgctgac
     2401 cgttgatgaa cgattggtag aaatggattt tggggattat gatggactgc caagcaaaga
     2461 tgagaacttt caaaaagcac gtttagcgtt cgctgtacga ttcccaaatg gggagtcagt
     2521 cttagatgtg tatgcacgga tcgttccttt attgaaagaa tgcatcgaag acgaagaaaa
     2581 tgtctatctg ttagtttgcc ataatgcctt gataagagtg atcaatgctt attttcatcc
     2641 gatgcctaat gaggggttct ttacattcat ggtagataat acagaattgg tttcttatga
     2701 gtaattatgt ttttgtgtta tttaaataaa atatttcaag gcactgcttt tgcttagcag
     2761 tgcctttttt aggttatgac aattcttttg ataatataaa ctcaaccttt agaaatgaaa
     2821 tttaattatt ttaataaaag tattgaaatt ttgtttcttt agatatatac tctagtcata
     2881 gaaatgaaga cggatacaga aaaagaggga taatatgttt ggcttttcaa tatttatgaa
     2941 cgattctttg tcagaagaga agaaacagta tatccgccaa atggcatcca atggctttgt
     3001 cggtattttc acttccatgc atataccaga agatgatgtt tcggcatatc aaaagcgatt
     3061 gatcgaacta ggaagcgaag cgaaaaagaa taaactagaa ttgatggtcg atatttcagg
     3121 agaggcattg gaacgagcgg gtttttcatt agaagatcta acgcctttga aagcaattgg
     3181 tgtaacaggt ttaagaatgg attatcatat ttccaatcag cagattgccg actggtcaca
     3241 tcaattgaaa atcagtttga atgctagcac gatcacgcct aaagatatcg atgaattgaa
     3301 agaagcagaa gctgatttct cacagattga agcttggcat aattattatc cacgtcccga
     3361 aacagggctg gacaaagaat ggtatcagaa aaaaaatcaa tggctcaaaa ttcaaggtct
     3421 ccttgtccaa ggttttgtac caggagatac agagttgaga ggcccactat atcaaggatt
     3481 gccaactttg gaagaacatc gagggatcca tcctcttgca ggcgctctag atctattagc
     3541 aagtaacacg gatattgttt acatcggaga tgcaggtctg tcagaaaacg ttcaggagca
     3601 atttgcttct ttccaaaaag aacagacagt cttgctccat acggaacctg tagatgaaga
     3661 attttacgaa tatatattag ggaagcatac gaatcggcaa gacgatgcaa gagatgtcat
     3721 acgaagcgta gatgcacgtt ttcgagagat tccgcctatc ccagcaagaa atacagctac
     3781 gcgtatgaaa gggtctatta ctttagataa cgaaaagtat ttgcgctata tgggtgagat
     3841 ccaattaacc aaatatgatt tgccagcgga tgaaaaagtg aatgtagtag ctaaagttat
     3901 caaagaagag ctaccattga tcaaccaaat caaagctggt atgaactatc aatttataag
     3961 aaaagaagga agataaaaat gagcaaaatc aatttagaga acctaacaac ggaacgcaga
     4021 aaccaagaaa cttttggatt agatgagatg agtatttctc aagccgttca actaatgaac
     4081 aaagaagatc agaaagtggc cgctgcggtt gaaaaagaac tgacaagtat cgaaccagtg
     4141 attgctaaca cgattgagtc ctttaaaaaa ggtggacgtc tgatttatat gggagccgga
     4201 acaagtggaa gactaggtgt gttagatgct gctgaatgcg taccgacatt cggtgtagaa
     4261 ccggaaatgg tcataggtct aattgctggt ggagaagaag caatgactgt agctgtcgaa
     4321 ggggccgaag attcttatga gctagggcaa gaagatctaa tcaagctgaa tttgacaaaa
     4381 aatgatatag ttattggtat cgcagctagc ggtcgtacac catatgtgat cggtggatta
     4441 gattacgcaa aatcgatcgg tgctgtcaca ggaacgattt cttgcaataa acaagccgaa
     4501 atcagtaaac acgcggattt cccaatcgaa gtcgatgcag gtccagaatt tttgacaggc
     4561 tctactcgat tgaagtcagg aacagcacaa aaacttatct tgaatatgat ttctactatc
     4621 agtatgattg gtataggtaa agtattcaat aacttgatgg tcgatgtcaa accaacaaat
     4681 gaaaaactaa tcgaacgctc aaaacgaatc atcatgcaag cgacagaagc tgattacgaa
     4741 acagctgaaa agtactttga agaagcagat caaaatgtta agctggctat cgtcatgatt
     4801 ttgacaaata gtgataaaga ggaagctcaa gcaaaattag ctgaagcaaa cggatttgta
     4861 aaagggacct tatgaggggg aaaagaccat ggcaaaaaac gaactgactt tggaagaact
     4921 ggcaaaacac atttataaag gggcaggcgg catgggcaat gtagaaagca ttgtccactg
     4981 catgactcgt gtgcgtatga agatcatcaa tgatgatctt gtaaatgtct ctgaattaaa
     5041 agcgattcca ggagtgcttg gtgtagtaga agatgaacag cttcaagtca tcatcggacc
     5101 tgggaaagtg aacaaagtag cacagcaaat ggttgatttg gcaggcgtga aattaggaga
     5161 aaaactttct tctgtaagta cagctacagc aaattcatca gcatctggaa aagaccgtgt
     5221 aaacgaacgg gcacaagaaa tgaaagcggc acaaaaatcg aaacaaaaac catctaaaat
     5281 caaatcggta ttaaaagaca tttctaatat ctttgtgcca atgattcctg catttgtcgg
     5341 tactgggatc gtggcaggga ttgccgcagt tttagcaaac ttagtcacag ccggaacact
     5401 tgatgcagca acatggcaac aatatatcga tgtgatgaac atcttgaaaa acgggatgtt
     5461 tagctaccta gttatctata caggtatcaa tgctgcacaa gtttttggtg caacaccaac
     5521 tcttggtgga gttatcgggg ccgttgtgat gctgacaggg atgaatccgg aagcaccact
     5581 gaaaaactta tttactggtg aagctcttgc agctggacaa ggtgggatcc taggtgtcat
     5641 cttcgctgtt tggctattgt caatcgttga gaaaaaactg cataaagtag tacctgatgc
     5701 ggtagatatc atcgttacac caactctttc attgattgcc atcggtctga ttgaaatttt
     5761 cttcatcatg cctttagcag gtttcatttc tgatggcatg gtcggtggga tcaactgggt
     5821 acttggcgtt ggtggagcat ttgccggctt cgttctaggc acattattct tgccgatggt
     5881 tatgttcgga ctgcatcaaa ttttgactcc aatccatgtg caaatgattg atgaaactgg
     5941 tcgtacgcta ttgcttccaa ttcttgcgat ggctggtgca ggacaagtgg gtgcagcatt
     6001 ggcattatgg attcgctgca aaaaagataa agaattaaca gaaatgatca aaggagcttt
     6061 accagtcggg atcctaggaa tcggcgaacc attgatttac ggagtaacat tacctctagg
     6121 acgcccattt attactgctt gtatcggtgg aggtatcggc ggggcagtga tcggaatgat
     6181 cggaaatgtc ggggctatcg ctattggacc ttcaggagcc gctttgatcc cattgatctc
     6241 tgacggtaaa tggttaggct acatccttgg attattggca gcttatgctg gtggcttcgt
     6301 tgctacattc ttcttcggta ttcctaaaga ccgtttagaa aaagaagaat tagcgactga
     6361 aacagccaca gtaaatgaag tgcctgaaaa agtagtacct caaacttcag ctgaaagtgt
     6421 gactatcctc tcatcagttg ctgacggaac agttgtttct ttagataaag caagtgatcc
     6481 agtttttgca caaaagatga tgggtgaagg ctactttgta gaaccagaaa acggacatat
     6541 ctattctcca gttactgggg tagtcagctc tgtcttccca acgaaacacg ctattgggat
     6601 cacaacgcca aatggtttag aaatcttgct tcatatggga atcaacacag ttgacttagg
     6661 cggtacgcca tttgatgtga aagtaacaga agggcagcaa gtgacacctg atacgcttgt
     6721 agcagaagct gacttagaag cgatcaaagc agcaggcaaa gaaacatcca tgatcgtctt
     6781 ggtaaccaat atggatcgtg taaaaaactt tgtcttggaa aaaacaggta aagcaaaagc
     6841 aaaagcacct gtgatggatg tcaaagctta atatgataga aacaatagaa acgaaagaag
     6901 aaactgaaat ctatttttca gcttcttctt ttttattatt tattgttttc tgctaaaatc
     6961 tgataaattc gtgtcacatc agatggtgtt ccccgtaatt cataatctgc caagaaagga
     7021 aagccaaatg tttgtgcata ttgtttggct gttagacaat actgattatt gaagttttta
     7081 tttccgctac cgactacacc aagacaaagt ttatgattgt cttcatgttc cagatattca
     7141 cgcatcgttt cggtcaagat ttctgtatca ccgttatcta aaccatttcc gccgtctaaa
     7201 taagtaggaa cgaaagtaaa aaatggttct gtttctttcg taaaatcact attttcatgg
     7261 atttctttta aagtaacttc aggattgaca ggatcttctg catgcatttt ttcagcatat
     7321 tctgctagat gtttggcaaa ggcacgcgta tttcctgaaa tcgatatata taaaatgttc
     7381 atggatcatc ctccaattct ttccttttag tatagcatct tttttaagtt ttttggtggt
     7441 gtaatttgga aagggactag gcgctttgct tgtataatga gaaaaaagcc ggtagaatta
     7501 tatttctatt aaagttttta ttaattaagt aaaaataaaa aaggtttgtt atactttttg
     7561 ataaaagcgg ttttattttg gaggatgctt acatggatac agagcaacag aaaaaaggaa
     7621 ttggtttaat tccacttgct gccttagtaa ttggttcagc gattggaggt ggcgtatttg
     7681 gtattatgac ggacatctcc ggatcggctg gcggcccagc tattttcagt tgggtactcg
     7741 taggaacagc aatgttgatg ctctccttgt cgatcaataa tatcaataaa aaacgtccag
     7801 atttaaaagg cgggatcttt agttacgcag aagaaggttt tggccgtttc gctggtttta
     7861 tcagcggttg gggttactgg ctcacatgct ggttaggcaa cgtagctttt gcaacattgt
     7921 tgatgagcgc attaggatat ttcttcccag tatttgaagg tgggcaaaat ctggcatcga
     7981 tcgtgatgag tacagtcttt ttatggggat atgcatggct agtgaatcgg ggagtcgaaa
     8041 atgctagttt cgtcaatgct atcgtcacat tctgtaaatt agtgccatta tttttattta
     8101 ttgtagtagc tatcagcacg ttttcgatcc aacaattcat tgataacttt tctagcgaca
     8161 ttatattgaa tgcgaatgga cagccgatcc cgttttccag tcaagtattg agttgcatca
     8221 tggtgatgct ttgggtattt gttggtattg aaggagcctc tgtcgtaggt tcacgagcaa
     8281 aaaaacaatc agatgttggg aaagcaacga ttctagggat attcggcttg attattattt
     8341 acgtcttggt ttccatgctt ccatatggga caatgacgat gaatcagctg caaacgatca
     8401 aggcgcagcc tgctatgggc tatgtgtttg aaatgatggt tggtaaatgg ggagcagtcg
     8461 tgatcaacgg cggtttgatc atttctttag ttggtgtttg gctctcatgg acgatcttac
     8521 cgattgaaac aatgggaaac atggctgaag acggactttt gcctaaagca tggggaaaac
     8581 tcaacaaaaa tggggcacca acttatgcta tcgtattgac gacgttatgt acgaatgtct
     8641 ttttactttc attattattc actgacagtg cttataactt tgcttacaca cttggtacag
     8701 cagctatttt cttcacgtgg cttttcttag gattgtacca aatgaaactt tcttatcagc
     8761 ggaaagaatg ggtccaattt ttagtagggc ttttagcaag tgcctttcaa gtatgggcta
     8821 tgctagtcgt agcctttaat gaagtaatgc ttgttcttgt tcttttcttg ccaggcattt
     8881 atttttacct caaagcacgt aaggagcaaa acgagcctgt acaacatatc cacttagata
     8941 agattttact tataggtgtg acgatggtcg gagtgattgc aatggtttta tttgccactg
     9001 ggatgcttca cgtatagaag tataaaagaa gtataaagag aggttgtgag cctgtcgttt
     9061 attcaaaaac atactaggaa aggagctgtg acacaaatgt tgtcacagct cctttttcag
     9121 aataaacggt gtttaagaga cagccaatcg gcaataaggc tgaacgtctc tttcacagcc
     9181 tcttttttga tgagtcatca tacatttttt cgcttaaata cgagatatcc taatgctaag
     9241 aacaaaacga tgtaagctaa attaccgaat agcatttgat tagtagaaag atgagtcaat
     9301 tgcttcatga tctcttcgtt tccaatttgg ttttgcagat tcaacatact gatcggattc
     9361 catttgatcc actcccattt ttggatgagt gcaataagga taccagatat gattgacgaa
     9421 gcaaaataaa agacgattcc tgctgagatg gatgcgccgg aactattgat aaagcaagca
     9481 atcatcaaca ctaaacttaa aatcagccat aaaccgatat aactacccaa agtataagtg
     9541 aacaagcttt ggatcagtgt ttgaccagta gaaaccttct ccgtgaagga aatcgaaggg
     9601 aaaagaatca gtttcatcaa cacagtgaca ataatagcta aaaggtataa ataaacagaa
     9661 taaataacta atgtgatcca tttgcttact aatatttctc ctcgagaata ctttcgataa
     9721 aggagatttt tcaatgtacc atattgtgct tccatcgaaa tgatcgtgct tgcagctgca
     9781 atcatgataa agacaatcca agatgtggca ctaaaaagct gagcagtcat ttcaactgga
     9841 tcaatgatcg tcgtgtattt tttggctaga agtgctgttc ctatgagcaa aacaactaat
     9901 agaacagata aaacagcagt tgactttttc ttgatcaatt taaataattc ttgttttaca
     9961 agcaatgtca tttttcttct tcccccttta ctgaatccag aatttccaat agagaagatt
    10021 ccaaatcatt cgtcagatgc tggacgtctg tcacatggat attttgtgca gacaatagct
    10081 ggatcacggt tcctaataat tcttcgtttt cttttaaaag cttgatttta tcaggatctt
    10141 ccaaagtata tccagcttct gtcaagatcg ttcgagcagc tagatcattt gttgtagaga
    10201 taacgatgaa atcttgcgct gtttggttca attcagccat tgtacattgt ttgatgatct
    10261 ttccttgatt gatcaggatc agctcatcga ccattttttc aagctcgctg agtaagtgac
    10321 tggagatcaa gaaagaggtg ccctgagtag ccaagtcggt aatgattttt cgcacatctc
    10381 ttgttgcttg tggatccaaa ccgttcatcg gctcatctaa aataacgata gacggatggt
    10441 tcagtaaggc aagagcaatt cccaattttt gtttcattcc caacgagtag gatttcgctt
    10501 ttttgtgaat gtacttttcc atcttgagct gattcacgat agtgtcgata gcagatacat
    10561 cgttggtttc agagaataac ttcagatgat catagcctgt taagaatgga tagatacccg
    10621 ggtattcgat caatgctcct acttgttcca accctttatg actagaggga gaaatctcat
    10681 tttcaccaat acagattgtt ccatctgagt aatggatcag tcctaaaata gctttcatga
    10741 tcgttgtttt tccagcgcca ttaggaccta ctaatccaac gattttgcca ggggataagc
    10801 taaaactgac atcgctgagg gcttgtttgt gaccaaagtt cttggataat tcttggaccg
    10861 aaagactttt caaaaatatt cactcctttt ctatagatag tacaagcata cacctgatga
    10921 gtggagaagt cgctaggtca ttggacttat ttcttatttt ttcataaaaa aattgaatat
    10981 taaataaaaa ggcttatttt ttctgatcag tacttattcc aagtgtctag tggccgctag
    11041 taatcttgat gccaatgata ctgatgagta gcatccccac aaagaaccaa gtggaaagat
    11101 tcagcttatc atgaaacaag accacaccaa ttatgaccga accaactgcg ccaatccctg
    11161 tccagatagg ataagagaga ctcatcggca aacttttgat tgcctttgac aaaaagtaaa
    11221 aactagcaat catgccaatc actgtgtaaa aagaataatt gattttagaa aaaccatcac
    11281 tccatttcat catcgttgac cagaatactt ccaaaattcc tgctatgatt aattccatcc
    11341 atgccatact tacattcctc cttaactata aaaaaataca caaaaagagg aaaatcatcc
    11401 cttcaaagac ctaatggaat gatcatcctc tttatcctac ccggtggcag attgtatatc
    11461 tttttctgta gtatagcaca cctatccaga tatcggcaat ctcatacttt ttagtaaagt
    11521 gtaccaagca ctgacggata tatcgttagc ggaaaagaag atgaatgctt tgcaatttca
    11581 tagaaagaag ttgttattta ttaatcattt aaaaaaaaca gtaactagac cttatttcta
    11641 tatattatcg ggcaataaat taaaaaaagc tttaccttac aataagtaag ataatttttg
    11701 tgataaaaaa ataacgaaaa atatttaaaa atagtgtcta tttcgtgaga aaacaactat
    11761 attttttatg tgttttgctt tataattaaa aaaatattgt cattttatgg aggaaaatat
    11821 atggaaaatt atcttagcct tttaggcgtc ttgattgtta ttctcgggtt tgcatttaaa
    11881 ttagactcga tattgatcgt aatggttgct ttagttgtta cagctttgac tggcggtctt
    11941 ggcattcaag gaatgttaga acttttaggt acaagttttg taaacaatcg cgggatggcg
    12001 atttttatca tcattatgtt ggcaacagga acgcttgaaa gaaatggtct gaaagaatca
    12061 gcggcttcct tgatcaagcg attcaagaaa gtatctgctg gtatgattgt agatatttat
    12121 gggatctttc gaatgatctt tgcggctttt aacgtgagtt ttggtggagt tgctggattt
    12181 gttcgtccaa tcattttacc gatggcattg gggaccatcg aatcaaaagg gttagaagtc
    12241 catcctaagc atgaagaaga actaaaaggt atggcttcag ctatggaaaa tgtctgctgg
    12301 ttctttggcc aagtactatt catcggagga gcaggggcgt tgctagtcca atcaacactg
    12361 aaagatctag gatatgacgt aacattggga cagcttgctt tagtggaagt tccagtggct
    12421 atcacggctt tagttgtggc aagtgtctac ttctatctta aagaaaagaa attagcgaag
    12481 aaatattatc caggaaaggg gcaataagat ggactttttt acaaatcctg atattttatt
    12541 aggcgataaa ttactggaga tcctttatat cgtgatggga ctcgtattga tttacactgg
    12601 agtaagaaat ttaacagata aaaccaatcc tcatcgttat gggtcagcct ttttctggac
    12661 ggtcttaggg gtagttattg caggaggaag atggcttccg gcaatcgtga acggtggctt
    12721 gatctttgcg atgaccatac cagcaattgc taagaaagtc agcaaaggag aaagccggct
    12781 tccatcaaaa gaatatatgg aaaaaatgtc agacaaacta ggaatgaaaa tttttattcc
    12841 agctttgagt atcggtgtct ttgcgatctt gtttgctcta tttaccaatt taggtgcttt
    12901 agtcggcgtg ggcgtcggtg tttttgcagc catgctaatc ttgatgttct tttctcgaga
    12961 taatcgacca agtacatttt tagatgatgc agcagatatg ctgggcacag tagggccgtt
    13021 aagcatgttg ccaatgcttt tagcttctct aggtgcggta tttacaagcg ctggagttgg
    13081 gacagtgatt tccaatgcag taggtacgat cgttccagaa ggaaatgtga acatcggcat
    13141 catcatttac gctcttggca tggtgatatt tacggtaatc atggggaatg catttgcggc
    13201 catcaccgtg atgacggttg ggatcggtgc accattcgta tttagtcatg gagcgaatcc
    13261 agcgttaata ggaatggttg cattgacttg cggtttttgt gggaccctgc tgacaccgat
    13321 ggctgctaac ttcaatatcg tgcctgtagc gatgctggag atgaaagaca aatacggagt
    13381 gatcaaaaac cagttgttta ttgcgttatt tatgctagtt ttccaa
//
LOCUS       DACEDM010000206         7929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.097, whole genome shotgun sequence.
ACCESSION   DACEDM010000206 DACEDM010000000
VERSION     DACEDM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.097"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            33..599
                     /gene="amaP"
                     /locus_tag="IWO50_002933"
     CDS             33..599
                     /gene="amaP"
                     /locus_tag="IWO50_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0359972.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            612..800
                     /locus_tag="IWO50_002934"
     CDS             612..800
                     /locus_tag="IWO50_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0359973.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            813..1370
                     /locus_tag="IWO50_002935"
     CDS             813..1370
                     /locus_tag="IWO50_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0359974.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1402..1644
                     /locus_tag="IWO50_002936"
     CDS             1402..1644
                     /locus_tag="IWO50_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0359975.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            1985..3199
                     /locus_tag="IWO50_002937"
     CDS             1985..3199
                     /locus_tag="IWO50_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0359976.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3230..3448
                     /locus_tag="IWO50_002938"
                     /pseudo
     CDS             3230..3448
                     /locus_tag="IWO50_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3867..4340
                     /gene="dfrF"
                     /locus_tag="IWO50_002939"
     CDS             3867..4340
                     /gene="dfrF"
                     /locus_tag="IWO50_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0359977.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            4467..5123
                     /locus_tag="IWO50_002940"
     CDS             4467..5123
                     /locus_tag="IWO50_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0359978.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            5305..5835
                     /locus_tag="IWO50_002941"
     CDS             5305..5835
                     /locus_tag="IWO50_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0359979.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            6381..6623
                     /locus_tag="IWO50_002942"
     CDS             6381..6623
                     /locus_tag="IWO50_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ0359980.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            6620..7090
                     /locus_tag="IWO50_002943"
     CDS             6620..7090
                     /locus_tag="IWO50_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0359981.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            7218..>7929
                     /locus_tag="IWO50_002944"
     CDS             7218..>7929
                     /locus_tag="IWO50_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0359982.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQK"
BASE COUNT         2750 a         1157 c         1660 g         2362 t
ORIGIN      
        1 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
       61 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      121 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      181 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      241 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      301 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      361 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      421 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      481 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      541 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      601 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      661 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      721 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
      781 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
      841 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
      901 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
      961 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1021 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1081 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1141 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1201 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1261 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1321 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1381 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1441 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1501 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1561 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1621 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1681 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1741 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     1801 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
     1861 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
     1921 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
     1981 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
     2041 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
     2101 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
     2161 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
     2221 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
     2281 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
     2341 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
     2401 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
     2461 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
     2521 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
     2581 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
     2641 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     2701 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     2761 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     2821 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     2881 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     2941 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     3001 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     3061 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     3121 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     3181 tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca tgcaacaaag
     3241 caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc
     3301 aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt
     3361 tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat
     3421 ggcaacggtg aaaggatggg aaaaaggtgt ttcccaaaca aaaagatatt aagaaacagc
     3481 ccatgtttac ctgcttgcaa acaaaagaat attgctacta gactaagaga tactacataa
     3541 gtgtgttggt atctcttttt tgttgccgtg aaacagcaga ctatttcatt tctgcttgat
     3601 taccttaaaa cttattagtc tatggtttgt caagggcttg ttgcgtgaga aatgctgaat
     3661 atcacccttt acaaaacata ggctgataag taacttttca aaagcagaaa agaaatggca
     3721 atgcttataa caatatgata taatgcagat gaagacaaaa aaggaatttg tcgagctggt
     3781 tagttcgaga taattttgag tttgttaaga tgtggtgcac ttgatggaat cacaggtacg
     3841 acatcatatt atggaggatg aaatagatga taggtttgat tgttgcgagg tcaaagaata
     3901 atgttatagg caagaatggt aatataccat ggaaaataaa gggagaacaa aagcaattta
     3961 gagagttaac aacgggtaat gtggttatta tggggcgaaa gtcttatgaa gaaatcggtc
     4021 atccgttgcc taatagaatg aatattgttg tttccaccac aacagagtat caaggagata
     4081 atttagtttc agttaaatca ttagaagatg cattattatt ggctaaagga cgagatgtat
     4141 acatatctgg tggatatgga ctatttaagg aagctttgca aatagtagat aaaatgtata
     4201 tcacagaagt agatttaaat attgaagatg gagatacatt ctttccagaa tttgatatca
     4261 atgattttga agttttgata ggggaaacac ttggtgagga agtgaaatat acgagaacat
     4321 tttatgtaag gaaaaaatga attgagtaaa ttttggattt agggtgtttt cataaatata
     4381 ttgcttttta tgcatatata aattgttgta agacaaattc cagtttgtat ggggcgataa
     4441 aatcagaatt tgagagagga ttgaatatga ataaggaatg gtctgaactt aataaaacaa
     4501 tgcaggcaca aataaaaaag aaagatactt ataagagggg tattgatact ttacttactt
     4561 tacgaagtca gttaatacaa accttagtat ctttcaaaga agaattatgc agagaagatt
     4621 ttaactcaat ccccttcata aacgctgatg gttatcatag taagacgatt gcttattcta
     4681 tttggcatat tttccgcatt gaagatatag ttgtgcatac agtgataaat gaagatgaac
     4741 aagtattttt tgcaggcaat tatcaagagc gtatcaattc acctatcatt acaacaggaa
     4801 atgaactcat gaaacagcag attgcagact tttcaaaaca gcttaatctg gaagaattat
     4861 atttatatat tttcgaagta tgggaaagca ctgagaaaat gcttgaacgg ttatcttatg
     4921 atgaattgaa aagaaaaata ccgaaagaaa gaaaaggata cttagaatcg ttgaatgtag
     4981 taaacgacaa tgaaaaagca atttggctga ttgattattg gtgtaataaa gatatttgtg
     5041 gactaattca aatgccgttt tcaagacatt ggattatgca cacagaagcc tgtttgcgta
     5101 taaagaacaa gatacattca taggcaaaaa tttcagtttg tagaactaag gaaattggaa
     5161 tttaaggagg aataaagtat ggttagtgat tttataagag cagcatttcc atggatttta
     5221 atgggtttat ttgtagcaat cagttgttcc cttatgagta aaaaagagaa ataaattcct
     5281 gtttgtattt ataaaagaga aaaaatgaga aaagtagttt tatttattgc catgagcctt
     5341 gacggatata ttgcggacgg taatggtgga gtagcttggc taaatggtca tggaaatgac
     5401 aatgaaaaca tcgacactta tacagaattt accaaagata tagatactgt cttaatgggg
     5461 tggaatactt ctcatcaggt tgttactgaa ctttctccgc aagaatgggt atataataaa
     5521 tttacaactt atgtattaac acataaagag tgcaattctt cccaacaaat tcattttaca
     5581 agtgaaaatc ctgttttatt attggaacgg ctaaaacaag aagcaggaaa ggatatttgg
     5641 atttgtggcg gagcaaatct tgttcagcag ttggtaagca aaaatatgat tgataaatat
     5701 tacatttctg ttattcctac tctgttagga aatggggttc gtcttttggg caacatagac
     5761 agggaaataa agttaaggct ttgtaaaaca cagacttata acggaataac cgatttgatt
     5821 tatatgcgta gataactaga gagaaacaga aaagctgtta aaccgacgga gcgatccgac
     5881 cacgttggcg ggaaaatcct taagattttt ccgccatgct tgcaaggggg cggttcgctt
     5941 cggcggttcg gcgagcttgc gagccgttca aagcccccgt tatctaacaa ggggggcaca
     6001 aaaaacaaga aacactatac actaacaagc ggaaaacgct gtatttataa ggcaaaaccc
     6061 catttttaca gtagtgatat aaattgtacc tatatcacta tgaaaattga atagtaaata
     6121 tgagaaagac agttgatttc aaaaaataag aaattgattg tcgttttctt ttgcagaaat
     6181 ttaagtgcag gcaattatgg tagttgtcgt aattgctgtc agctcagtgt ttatcactga
     6241 tggtgtgaaa gatttacggt tggagatgtt caataagatt attggtgctt aattattttt
     6301 attgtagtgc tgatatgaga atgttataat tggggtataa aacatacaaa taaatttgct
     6361 gaaaaatgag gtatcaaaat atggaaaatg aattactgga aaacttagga agacttcaca
     6421 caactgaact tggcgttatt agaattaaaa agaacttatc tttgaatgta gaaaatgtaa
     6481 ttgaatggtg caaagaaaaa attagccttt ctaatgcgga aatcacaaga aaaggaaaaa
     6541 actggtatat aacgattgat aattgtatta taacggtaaa tgcgtacagt tataccgtta
     6601 tcactgctca caaggtaaaa tgataaattt ttctataaag gaattaaacg catttgcagt
     6661 tattgggcag gaagtagaac tgactaatta tcaaaaaaag aatattcaga ttagtacaca
     6721 gttttggaga aagtttaaca gtagtttgaa aaaagcgtat ctttcacaat caggaaattg
     6781 ggtaaaatat gcttttatgg aaagaagaaa tggaaaactt tattatttct gttctattcc
     6841 ccaaagaacc attaccccag agggctttct gtataaagaa ataccgtctt ataaatattt
     6901 ggttgtggag catattggtg ctatggataa aatatatgaa acttatggca agatttatga
     6961 agaaattatt cctagtacat cgtatattcc tataaaagat attatcctac actttgaaaa
     7021 atacgattat cgttttcatt ggaatagtga taattcagtt attgaaattt ggatacctat
     7081 tcaagattag aagcaatttt atatccatac acacaaagca gttagtctta ggattgactg
     7141 ctttttctat acagattttt aatgacaggc aattatcaat tatggtagtt gccttttttg
     7201 attggaggaa ttgaagaatg aatgataaaa actttattga agaactaaga caaaagcgtg
     7261 aggaatacgg agtaacacaa acaaggcttg ctgttgcctg tggtatcagc cgtgaatatt
     7321 acaaccgcat tgaaaaagga aaacagccat tgaatgacga attaagagaa atcatagaaa
     7381 aacagattga gcgtttcaat ccgcaagaac cactattctt attgattgat tactttcgtg
     7441 tccgctttcc tactacggac gcattggcga ttatccgtga tgtattacaa ctgaaatcta
     7501 attacatgct ttatgaagat tatggaaaat acggatatga aagcaaatat gtacttggcg
     7561 acatcaatat catgtgttcc atgcaggagc atttaggtgt tttattggaa ctgaaaggca
     7621 aaggctgtcg gcaaatggaa tgttatctgc tggcacagga acgctcatgg tatgacttca
     7681 tgctggattg tatgacggct ggtggtgtga tgaaacggct tgatttggct atcaatgata
     7741 aagcaggaat attggatatt ccaaagttaa aggaaaaata caaggctggc gaatgtatgt
     7801 cctacttccg tatgcagaaa gattacagcg gtacagaaaa atgcggtagc gatttaccaa
     7861 agaatacagg agaaacctta tatctcggtt caacaagtag cgaattatat atgtgtgctt
     7921 atcagaaaa
//
LOCUS       DACEDM010000207         7707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.101, whole genome shotgun sequence.
ACCESSION   DACEDM010000207 DACEDM010000000
VERSION     DACEDM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.101"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..201)
                     /locus_tag="IWO50_002945"
     CDS             complement(<1..201)
                     /locus_tag="IWO50_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ0359983.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGV"
     gene            complement(459..830)
                     /locus_tag="IWO50_002946"
     CDS             complement(459..830)
                     /locus_tag="IWO50_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0359984.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            975..1160
                     /locus_tag="IWO50_002947"
     CDS             975..1160
                     /locus_tag="IWO50_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0359985.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            2103..2762
                     /locus_tag="IWO50_002948"
     CDS             2103..2762
                     /locus_tag="IWO50_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAQ0359986.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2765..4108
                     /gene="vanS"
                     /locus_tag="IWO50_002949"
     CDS             2765..4108
                     /gene="vanS"
                     /locus_tag="IWO50_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0359987.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            4279..5085
                     /gene="vanY"
                     /locus_tag="IWO50_002950"
     CDS             4279..5085
                     /gene="vanY"
                     /locus_tag="IWO50_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0359988.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            5103..5930
                     /gene="vanW"
                     /locus_tag="IWO50_002951"
     CDS             5103..5930
                     /gene="vanW"
                     /locus_tag="IWO50_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAQ0359989.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            5927..6898
                     /locus_tag="IWO50_002952"
     CDS             5927..6898
                     /locus_tag="IWO50_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0359990.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            6891..>7707
                     /locus_tag="IWO50_002953"
     CDS             6891..>7707
                     /locus_tag="IWO50_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0359991.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQ"
BASE COUNT         2179 a         1637 c         1988 g         1903 t
ORIGIN      
        1 gacgccgatc ttctgtatct cggcggtctg cgcccggatg tcgctggtgt ccatgttgac
       61 gatatagccg tcaatgagca tcttccgggc gtaggctgaa aagttcttcg tatgcagcag
      121 cgccattttc tgttcaatca tagcccgttc ctccggcgtc acagggacgc gcagcacgat
      181 ttttcgcttt ctgtttgcca tgcttcaccg tcctttccat tcaggggttt ggggatttcc
      241 ccaacaagca aaacggacgc ggcccggcag ggacggggca ggccgttttt ccgggagtgg
      301 ctacactccc tatgcttgct acgttactgt ttctccacta acaagacgtt tccaaagccc
      361 caaactaccc gcaaaatagc cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc
      421 tgtgcagctc ccgctaaaag taaaacagag gatgccagtt acacatcctc cgtttcattc
      481 ttcttcggct tttcaattag tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat
      541 tcttcggggc agagtttcag cttgtggctg accctttgcc gcagttcgct ttcctcccgc
      601 atgatctccg ggttaaagta gcgttccaca ggaagcccgc aggctttaat aagctgtatg
      661 acaaccggca gactgggaat ggccccgtcg ttttcgatgt tggcaagata gcgccagtca
      721 atatttattt tctctgccat tgctttgcgc gaaacgcgct ttgcttgccg ggccgctttc
      781 acatccgcgc cgaaagtttc aaagccggga caatcttcaa tattcgccat atcgcacctc
      841 ccacttacat tgtatatttc atactctgcc cgtggaatgt tggtatatgc acctttctta
      901 aagtttatat ttcacatttt gtttctttta ttcttcgagc aaaagaatta taatgtaccc
      961 tgtggaggtg tactatggac tacatgacgt tgaaagaggc aggcgagaaa tggggtgtgc
     1021 ccccacgatg gataaactac tattgtgctg cgggacgcat ccccggcgct gtaaagatgg
     1081 cgacgatctg gctcattccc aaaactgcgg aaaagccgat tgacaggcgt acaaagaaag
     1141 gaaaggcggt tactgaatag tataatgaaa ccggcgcatt ttggatatgg ttttgaagag
     1201 agagaaagca tggtattttg tcacggaaaa actataagat gatagatggc aggctgctcc
     1261 agaccgataa aaagtattct catctgaaac agaaagaaaa gatttacaca tggatgtatg
     1321 aagaagcgaa actttatcat gacagaaatg gaaaatgccc gaaacaaaga cgaggaagct
     1381 gctgctgtat cagccgtata tggtagaatt gaaaaggccg gcatctggat tctttatggt
     1441 gaagtctcca gacattggat aactgctggt ctaatgcgcc tttgagtatg gggtgattga
     1501 ttttaatacc ggggaggtac ggctttttga ggaaaatatt ctgggattta catttggcga
     1561 ttattacatt tcctgtaact gcaaggacac ggatgatgaa gatatgagga atatagactt
     1621 gctgattttc tactgtcttg aacggtatta ggactgaatc tgctgcgcgg agtgcggcaa
     1681 gagcgaccgg agggagtaca cacaggccct gcctgtgtga aaagccatac cgcaaggtgc
     1741 attcgcaaaa aagaggccgc gcattttgct gaggcttggg gtgtccccaa caagcaaaac
     1801 gagaacgccg caggcgggca agtggtggat cagaatatcc gtaaaggata ttctgatcca
     1861 ttgcttgcca gtattatcgg gatgttttga caaccgctgt aatcgaaaca tcccaaacat
     1921 taagaaggtg ttaattatta ttatcttgat ttaattcgtt taaaccaata atatagatac
     1981 atacaccatg aaaagaagcg agtatcccaa tgacggatga ttaaaatccg aaaagaaatc
     2041 gctttgaaac tacagggaaa ctacagactg ttatgttatc ttcttaaaat ggagggattt
     2101 ttatgtcgat acgaattcta cttgtcgagg atgatgatca tatctgtaat acagtaaggg
     2161 cgtttctggc tgaggcagga tatcaggtgg atgcctgcac agatggaaat gaggcataca
     2221 ccaagtttta cgaaaacact tatcaactgg ttattcttga tattatgctg cccggtatga
     2281 acgggcatga acttttgcgt gaatttcgtg cgaaaaatga tactcccatt ctgatgatga
     2341 cagccctgtc ggatgacgaa aaccaaatcc gggcgtttga tgcagaggca gacgactatg
     2401 taacaaagcc attcaagatg cagattttac taaagcgggt ggaagccctg ctgcggcgca
     2461 gcggtgcgct ggcaaaggaa atccgtgtcg gcaggctgac acttctgccg gaggatttca
     2521 cggtactttg tgacggtaca gagctgcccc tgacacgaaa agaatttgaa atccttttgc
     2581 tgctggtgca gaacaaaggc agaaccttaa cgcatgaaat cattttgtca cgtatatggg
     2641 gatatgactt tgagggtgat ggcagcacag tccacactca tatcaaaaat ttgcgggcca
     2701 agctgccgga aaatatcatc aaaaccattc gcggtgtagg ataccgattg gaggaatcat
     2761 aataatggaa agaaaaggga ttttcattaa ggttttttcc tatacgatca ttgtcctgct
     2821 actggttgtc ggtgtaacag caacactgtt tgcacagcaa tttgtgtctt atttcagagt
     2881 gatggaatta cagcaaacag taaaatccta tcagccattg gtggaactga ttcagaatag
     2941 cgataggctt gatattcaag aggtggcagg gctgtttcac tacaataacc aatcctttga
     3001 gttttatatt gaagataaag agggaagcgt actctatgcc acaccaaatg ccaatacatc
     3061 aaatagtttt agacccgact ttctttatgt ggtacataga gatgataata tttcgattgt
     3121 tgctcaaaac aaggcaggtg tgggattgct ttatcaaggg ctgacaattc ggggaattgt
     3181 tatgattgcg ataatggttg tattcagcct tttatgcgcg tatatctttg cgcggcaaat
     3241 gacaacgccg atcaaagcct tagcggacag tgcgaataaa atggcaaacc tgaaagatgt
     3301 accgccgccg ctggagcgaa aggatgagct tggcgcactg gctcatgata tgcattccat
     3361 gtatgtcagg ctgaaagaaa ccatcgcaag gctggaggat gaaatcgcaa gggaacatga
     3421 gttggaggaa acacagcgat atttctttgc ggcagcttct catgagctaa aaacgcccat
     3481 cgcggctaca agcgttctgt tggagggaat gcttggaaat atcggtgact acaaagatca
     3541 ttctaagtat ctgcgcgaat gcatcaaaat gatggatagg cagggcaaaa tcatttccga
     3601 aatactggag cttgtcagcc tgaatgatgg gagaatcgta cccatagctg aaccgttgga
     3661 catagggcgc acggttgccg agttgctgcc cgattttcaa accttggcag aggcaaacaa
     3721 ccagcggttc gtcacagata ttccagccgg gcaaattgtc ctgtccgatc cgaggctgct
     3781 ccaaaaggca ctatccaatg tcatattgaa tgcagttcag aacacgccgc agggaggcga
     3841 ggtacggata tggagtgagc ctggtgctga aaaatgccgc ctttttgttt tgaacatggg
     3901 cgttcacatt gatgatactg cgcttccaag gctgttcacc ccattctatc gcattgatca
     3961 ggcgcgaagc agaaaaagtg ggcgaagcgg tttaggactt gccatcgtac aaaaaacgct
     4021 ggatgccatg agcctccagt atgcgctgga aaacacctcg gatggcgttt tgttctggct
     4081 ggatttaccg ctcacatcaa cattgtaaat atttaaaact taaatgattt tgaccgacag
     4141 gtataaccct gccggtcttt ttttgctgct acaggaaaac tacagactgg ctacagggaa
     4201 agtacagatg cgcttgccat aataacaatc gtaccagcca caaatcgtag ttttattgca
     4261 aaggaggcat tcgatcaaat ggaaaaaagc aactatcatt ccaatgcgga tcatcacaaa
     4321 cggcatatga aacaatccgt ggaaaagcgg gcttttctat gtgcgttcat tatttcgttc
     4381 acagtctgca cgctgttttt gaggtggaga ctggcttccg tattggaggc aacacagata
     4441 ccgcccatcc ctgcaactca tacaggcagc agcactgacg tagtggagaa tttggaggaa
     4501 aacgctcttg ccaccgccaa agaacaggga gatgaacagg aatggagcct gattttagtg
     4561 aacaggcaga accccatccc cgcacagtac gatgtggagc ttgagcaact atcaaatggt
     4621 gagcggatag atattcggat ttctccctat cttcaagatt tgtttgatgc cgcaagaact
     4681 gatggagttt acccgattgt cgcatccgga taccgaacaa cagaaaaaca gcaagaaatt
     4741 atggatgaaa aaattgccga atataaggcg aaaggctaca cctctgcaca ggctaaagcg
     4801 gaagcagaaa cttgggtggc cgtgccggga acgagcgagc atcagcttgg tcttgctgtg
     4861 gatatcaatg cggacggaat tcattcaaca ggcaacgagg tttatagatg gctggatgaa
     4921 aacagctatc gttttggttt tattcgtcgc tacccaccag acaagacaga gataaccggt
     4981 gtgagcaacg agccgtggca ttaccgatat gttggcatcg aagctgccac agagatgtac
     5041 aaccaagggg tttgccttga ggaatattta aaaccagaaa aatgagaaaa ggatgttatg
     5101 ttatggacag aaaaagattg acacagcgtt tcccgttcct gcttccaatg agacgggcgc
     5161 agagaaaaat gtgcttttat gcgggaatga gatttgacgg ctgtcgctat gcgcagacga
     5221 taggggaaaa atcgctttcc catttgctct ttgaaacgga ttgcgcatta tacaaccaca
     5281 ataccggatt tgacatgata taccaggaaa acaaggtgtt caacttaaag ctggcagcaa
     5341 agaccttaaa cggcttattg ataagaccgg gggaaacctt ttctttctgg tggctggtac
     5401 gccatgcgga caaagatacc ccctataaag acggccttac ggtgaccaat ggtaaactca
     5461 ccaccatgtc gggcggcggt atgtgccaga tgagcaattt actattttgg atgttcctgc
     5521 atacgccatt gacaattatc cagcgcagag gtcacgaagt aaaggagttt ccagagccaa
     5581 acagtgacga gattaaaggg gtggatgcaa ccattccaga gggctggatt gatttaaaag
     5641 tgcgaaacga taccgactgc acctaccaaa tatgggtgac cttagatgat gagaaaatca
     5701 tcggtcaggt gtccgccgac aaagagcccc aagcattata caaaattaca aatggcagta
     5761 tccagtatgt ccgtgaaagt ggcgggattt atgaatatgc ccaggttaaa cggatgcaag
     5821 ttgccttagg taccggggaa ataatagatt gcaagctgct ttatacaaac aaatgcaaaa
     5881 tctgttatcc cctcccggaa agtgtggata ttcaggagga gaaccaatga gaaaaagtgt
     5941 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
     6001 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
     6061 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
     6121 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
     6181 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
     6241 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
     6301 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
     6361 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
     6421 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
     6481 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
     6541 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
     6601 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
     6661 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
     6721 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
     6781 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
     6841 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     6901 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     6961 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     7021 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     7081 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     7141 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa ttcggggagg
     7201 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     7261 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     7321 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     7381 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     7441 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     7501 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     7561 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     7621 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttccc gcagacattc
     7681 cggtcgagga acgaaatcgg gtgcagg
//
LOCUS       DACEDM010000208         7707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.102, whole genome shotgun sequence.
ACCESSION   DACEDM010000208 DACEDM010000000
VERSION     DACEDM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.102"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..201)
                     /locus_tag="IWO50_002954"
     CDS             complement(<1..201)
                     /locus_tag="IWO50_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ0359992.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGV"
     gene            complement(459..830)
                     /locus_tag="IWO50_002955"
     CDS             complement(459..830)
                     /locus_tag="IWO50_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0359993.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            975..1160
                     /locus_tag="IWO50_002956"
     CDS             975..1160
                     /locus_tag="IWO50_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0359994.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            2103..2762
                     /locus_tag="IWO50_002957"
     CDS             2103..2762
                     /locus_tag="IWO50_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAQ0359995.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2765..4108
                     /gene="vanS"
                     /locus_tag="IWO50_002958"
     CDS             2765..4108
                     /gene="vanS"
                     /locus_tag="IWO50_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0359996.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFP
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            4279..5085
                     /gene="vanY"
                     /locus_tag="IWO50_002959"
     CDS             4279..5085
                     /gene="vanY"
                     /locus_tag="IWO50_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0359997.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            5103..5930
                     /gene="vanW"
                     /locus_tag="IWO50_002960"
     CDS             5103..5930
                     /gene="vanW"
                     /locus_tag="IWO50_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAQ0359998.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            5927..6898
                     /locus_tag="IWO50_002961"
     CDS             5927..6898
                     /locus_tag="IWO50_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0359999.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            6891..>7707
                     /locus_tag="IWO50_002962"
     CDS             6891..>7707
                     /locus_tag="IWO50_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0360000.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQ"
BASE COUNT         2178 a         1638 c         1988 g         1903 t
ORIGIN      
        1 gacgccgatc ttctgtatct cggcggtctg cgcccggatg tcgctggtgt ccatgttgac
       61 gatatagccg tcaatgagca tcttccgggc gtaggctgaa aagttcttcg tatgcagcag
      121 cgccattttc tgttcaatca tagcccgttc ctccggcgtc acagggacgc gcagcacgat
      181 ttttcgcttt ctgtttgcca tgcttcaccg tcctttccat tcaggggttt ggggatttcc
      241 ccaacaagca aaacggacgc ggcccggcag ggacggggca ggccgttttt ccgggagtgg
      301 ctacactccc tatgcttgct acgttactgt ttctccacta acaagacgtt tccaaagccc
      361 caaactaccc gcaaaatagc cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc
      421 tgtgcagctc ccgctaaaag taaaacagag gatgccagtt acacatcctc cgtttcattc
      481 ttcttcggct tttcaattag tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat
      541 tcttcggggc agagtttcag cttgtggctg accctttgcc gcagttcgct ttcctcccgc
      601 atgatctccg ggttaaagta gcgttccaca ggaagcccgc aggctttaat aagctgtatg
      661 acaaccggca gactgggaat ggccccgtcg ttttcgatgt tggcaagata gcgccagtca
      721 atatttattt tctctgccat tgctttgcgc gaaacgcgct ttgcttgccg ggccgctttc
      781 acatccgcgc cgaaagtttc aaagccggga caatcttcaa tattcgccat atcgcacctc
      841 ccacttacat tgtatatttc atactctgcc cgtggaatgt tggtatatgc acctttctta
      901 aagtttatat ttcacatttt gtttctttta ttcttcgagc aaaagaatta taatgtaccc
      961 tgtggaggtg tactatggac tacatgacgt tgaaagaggc aggcgagaaa tggggtgtgc
     1021 ccccacgatg gataaactac tattgtgctg cgggacgcat ccccggcgct gtaaagatgg
     1081 cgacgatctg gctcattccc aaaactgcgg aaaagccgat tgacaggcgt acaaagaaag
     1141 gaaaggcggt tactgaatag tataatgaaa ccggcgcatt ttggatatgg ttttgaagag
     1201 agagaaagca tggtattttg tcacggaaaa actataagat gatagatggc aggctgctcc
     1261 agaccgataa aaagtattct catctgaaac agaaagaaaa gatttacaca tggatgtatg
     1321 aagaagcgaa actttatcat gacagaaatg gaaaatgccc gaaacaaaga cgaggaagct
     1381 gctgctgtat cagccgtata tggtagaatt gaaaaggccg gcatctggat tctttatggt
     1441 gaagtctcca gacattggat aactgctggt ctaatgcgcc tttgagtatg gggtgattga
     1501 ttttaatacc ggggaggtac ggctttttga ggaaaatatt ctgggattta catttggcga
     1561 ttattacatt tcctgtaact gcaaggacac ggatgatgaa gatatgagga atatagactt
     1621 gctgattttc tactgtcttg aacggtatta ggactgaatc tgctgcgcgg agtgcggcaa
     1681 gagcgaccgg agggagtaca cacaggccct gcctgtgtga aaagccatac cgcaaggtgc
     1741 attcgcaaaa aagaggccgc gcattttgct gaggcttggg gtgtccccaa caagcaaaac
     1801 gagaacgccg caggcgggca agtggtggat cagaatatcc gtaaaggata ttctgatcca
     1861 ttgcttgcca gtattatcgg gatgttttga caaccgctgt aatcgaaaca tcccaaacat
     1921 taagaaggtg ttaattatta ttatcttgat ttaattcgtt taaaccaata atatagatac
     1981 atacaccatg aaaagaagcg agtatcccaa tgacggatga ttaaaatccg aaaagaaatc
     2041 gctttgaaac tacagggaaa ctacagactg ttatgttatc ttcttaaaat ggagggattt
     2101 ttatgtcgat acgaattcta cttgtcgagg atgatgatca tatctgtaat acagtaaggg
     2161 cgtttctggc tgaggcagga tatcaggtgg atgcctgcac agatggaaat gaggcataca
     2221 ccaagtttta cgaaaacact tatcaactgg ttattcttga tattatgctg cccggtatga
     2281 acgggcatga acttttgcgt gaatttcgtg cgaaaaatga tactcccatt ctgatgatga
     2341 cagccctgtc ggatgacgaa aaccaaatcc gggcgtttga tgcagaggca gacgactatg
     2401 taacaaagcc attcaagatg cagattttac taaagcgggt ggaagccctg ctgcggcgca
     2461 gcggtgcgct ggcaaaggaa atccgtgtcg gcaggctgac acttctgccg gaggatttca
     2521 cggtactttg tgacggtaca gagctgcccc tgacacgaaa agaatttgaa atccttttgc
     2581 tgctggtgca gaacaaaggc agaaccttaa cgcatgaaat cattttgtca cgtatatggg
     2641 gatatgactt tgagggtgat ggcagcacag tccacactca tatcaaaaat ttgcgggcca
     2701 agctgccgga aaatatcatc aaaaccattc gcggtgtagg ataccgattg gaggaatcat
     2761 aataatggaa agaaaaggga ttttcattaa ggttttttcc tatacgatca ttgtcctgct
     2821 actggttgtc ggtgtaacag caacactgtt tgcacagcaa tttgtgtctt atttcagagt
     2881 gatggaatta cagcaaacag taaaatccta tcagccattg gtggaactga ttcagaatag
     2941 cgataggctt gatattcaag aggtggcagg gctgtttcac tacaataacc aatcctttga
     3001 gttttatatt gaagataaag agggaagcgt actctatgcc acaccaaatg ccaatacatc
     3061 aaatagtttt agacccgact ttctttatgt ggtacataga gatgataata tttcgattgt
     3121 tgctcaaaac aaggcaggtg tgggattgct ttatcaaggg ctgacaattc ggggaattgt
     3181 tatgattgcg ataatggttg tattcagcct tttatgcgcg tatatctttg cgcggcaaat
     3241 gacaacgccg atcaaagcct tagcggacag tgcgaataaa atggcaaacc tgaaagatgt
     3301 accgccgccg ctggagcgaa aggatgagct tggcgcactg gctcatgata tgcattccat
     3361 gtatgtcagg ctgaaagaaa ccatcgcaag gctggaggat gaaatcgcaa gggaacatga
     3421 gttggaggaa acacagcgat atttctttgc ggcagcttct catgagctaa aaacgcccat
     3481 cgcggctaca agcgttctgt tggagggaat gcttggaaat atcggtgact acaaagatca
     3541 ttctaagtat ctgcgcgaat gcatcaaaat gatggatagg cagggcaaaa tcatttccga
     3601 aatactggag cttgtcagcc tgaatgatgg gagaatcgta cccatagctg aaccgttgga
     3661 catagggcgc acggttgccg agttgctgcc cgattttcaa accttggcag aggcaaacaa
     3721 ccagcggttc gtcacagata ttccagccgg gcaaattgtc ctgtccgatc cgaggctgct
     3781 ccaaaaggca ctatccaatg tcatattgaa tgcagttcag aacacgccgc agggaggcga
     3841 ggtacggata tggagtgagc ctggtgctga aaaatgccgc ctttttgttt tgaacatggg
     3901 cgttcacatt gatgatactg cgcttccaag gctgttcccc ccattctatc gcattgatca
     3961 ggcgcgaagc agaaaaagtg ggcgaagcgg tttaggactt gccatcgtac aaaaaacgct
     4021 ggatgccatg agcctccagt atgcgctgga aaacacctcg gatggcgttt tgttctggct
     4081 ggatttaccg ctcacatcaa cattgtaaat atttaaaact taaatgattt tgaccgacag
     4141 gtataaccct gccggtcttt ttttgctgct acaggaaaac tacagactgg ctacagggaa
     4201 agtacagatg cgcttgccat aataacaatc gtaccagcca caaatcgtag ttttattgca
     4261 aaggaggcat tcgatcaaat ggaaaaaagc aactatcatt ccaatgcgga tcatcacaaa
     4321 cggcatatga aacaatccgt ggaaaagcgg gcttttctat gtgcgttcat tatttcgttc
     4381 acagtctgca cgctgttttt gaggtggaga ctggcttccg tattggaggc aacacagata
     4441 ccgcccatcc ctgcaactca tacaggcagc agcactgacg tagtggagaa tttggaggaa
     4501 aacgctcttg ccaccgccaa agaacaggga gatgaacagg aatggagcct gattttagtg
     4561 aacaggcaga accccatccc cgcacagtac gatgtggagc ttgagcaact atcaaatggt
     4621 gagcggatag atattcggat ttctccctat cttcaagatt tgtttgatgc cgcaagaact
     4681 gatggagttt acccgattgt cgcatccgga taccgaacaa cagaaaaaca gcaagaaatt
     4741 atggatgaaa aaattgccga atataaggcg aaaggctaca cctctgcaca ggctaaagcg
     4801 gaagcagaaa cttgggtggc cgtgccggga acgagcgagc atcagcttgg tcttgctgtg
     4861 gatatcaatg cggacggaat tcattcaaca ggcaacgagg tttatagatg gctggatgaa
     4921 aacagctatc gttttggttt tattcgtcgc tacccaccag acaagacaga gataaccggt
     4981 gtgagcaacg agccgtggca ttaccgatat gttggcatcg aagctgccac agagatgtac
     5041 aaccaagggg tttgccttga ggaatattta aaaccagaaa aatgagaaaa ggatgttatg
     5101 ttatggacag aaaaagattg acacagcgtt tcccgttcct gcttccaatg agacgggcgc
     5161 agagaaaaat gtgcttttat gcgggaatga gatttgacgg ctgtcgctat gcgcagacga
     5221 taggggaaaa atcgctttcc catttgctct ttgaaacgga ttgcgcatta tacaaccaca
     5281 ataccggatt tgacatgata taccaggaaa acaaggtgtt caacttaaag ctggcagcaa
     5341 agaccttaaa cggcttattg ataagaccgg gggaaacctt ttctttctgg tggctggtac
     5401 gccatgcgga caaagatacc ccctataaag acggccttac ggtgaccaat ggtaaactca
     5461 ccaccatgtc gggcggcggt atgtgccaga tgagcaattt actattttgg atgttcctgc
     5521 atacgccatt gacaattatc cagcgcagag gtcacgaagt aaaggagttt ccagagccaa
     5581 acagtgacga gattaaaggg gtggatgcaa ccattccaga gggctggatt gatttaaaag
     5641 tgcgaaacga taccgactgc acctaccaaa tatgggtgac cttagatgat gagaaaatca
     5701 tcggtcaggt gtccgccgac aaagagcccc aagcattata caaaattaca aatggcagta
     5761 tccagtatgt ccgtgaaagt ggcgggattt atgaatatgc ccaggttaaa cggatgcaag
     5821 ttgccttagg taccggggaa ataatagatt gcaagctgct ttatacaaac aaatgcaaaa
     5881 tctgttatcc cctcccggaa agtgtggata ttcaggagga gaaccaatga gaaaaagtgt
     5941 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
     6001 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
     6061 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
     6121 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
     6181 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
     6241 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
     6301 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
     6361 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
     6421 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
     6481 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
     6541 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
     6601 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
     6661 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
     6721 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
     6781 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
     6841 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     6901 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     6961 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     7021 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     7081 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     7141 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa ttcggggagg
     7201 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     7261 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     7321 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     7381 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     7441 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     7501 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     7561 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     7621 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttccc gcagacattc
     7681 cggtcgagga acgaaatcgg gtgcagg
//
LOCUS       DACEDM010000209         7417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.111, whole genome shotgun sequence.
ACCESSION   DACEDM010000209 DACEDM010000000
VERSION     DACEDM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.111"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            396..1880
                     /gene="lsa"
                     /locus_tag="IWO50_002963"
     CDS             396..1880
                     /gene="lsa"
                     /locus_tag="IWO50_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0360001.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTEFMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LLKEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAAKAMKRAKNIESR
                     HQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDKEVCSNLNFRVNI
                     GDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDTSYLKGNLSEFA
                     YNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCESAHLYIWDEPL
                     NYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            1934..2737
                     /gene="lnu(B)"
                     /locus_tag="IWO50_002964"
     CDS             1934..2737
                     /gene="lnu(B)"
                     /locus_tag="IWO50_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAQ0360002.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            3425..4246
                     /locus_tag="IWO50_002965"
                     /pseudo
     CDS             3425..4246
                     /locus_tag="IWO50_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4389..4613
                     /locus_tag="IWO50_002966"
     CDS             4389..4613
                     /locus_tag="IWO50_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0360003.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            4628..5497
                     /locus_tag="IWO50_002967"
     CDS             4628..5497
                     /locus_tag="IWO50_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0360004.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5478..6212
                     /locus_tag="IWO50_002968"
     CDS             5478..6212
                     /locus_tag="IWO50_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0360005.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6245..7153
                     /locus_tag="IWO50_002969"
     CDS             6245..7153
                     /locus_tag="IWO50_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0360006.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7150..7398
                     /locus_tag="IWO50_002970"
     CDS             7150..7398
                     /locus_tag="IWO50_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAQ0360007.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         2587 a         1058 c         1531 g         2241 t
ORIGIN      
        1 aagggatttt attctttatt taaaactttg caacagaacc cttttcttgc taattatatg
       61 atgcataata agatatttaa agaaacggct atagaaccgt ttgttttgtt atgcaaattg
      121 caagaaagtt aagtattcta cactcaataa tacattacag taaccttatg ttatgtataa
      181 caaaaggtaa taatagtatt tgtttttgag ccgtaagaat ttactttctt gcggctttta
      241 atatttgtaa aagaaaggaa gataaacgtg atagacaatg atactatgtc attcatgaca
      301 aaatccttgc agagttggat aacattatag agtaattaat gccgaaaacc gactttgctt
      361 ataaaatttt tataatgtaa aggaggaaga aatatatgtc cttaataaat gtttcaaatc
      421 taactttttc atatgaagga agttatgaca atatttttga aaatgtaagt tttcagatag
      481 atacagattg gaaactcggt tttattggaa gaaacggacg cggtaaaact actttcttaa
      541 atttactgct tggcaaatat gcgtattccg gcaatataag ttctacagtt aagtttgagt
      601 attttcctta tgatgtggaa gataagagtc tatatacaat tgaagtaatg aagagtattt
      661 gtacggaatt tatggattgg gagatttttc gtgaaatatc attgcttgat gttcaagaag
      721 atgctttata tcgtccgttt aatacattgt caaatggtga gcaaacgaag gtccttcttg
      781 cagctttatt ccttacagcg agttgtttcc tgcttattga tgaacctaca aaccatcttg
      841 acatcgatgc acgtaatgta gtgcaaaact atttgaaacg caagaagggg tttattttgg
      901 tatctcatga tagaagctta cttgatcaat gtgttgacca tatactatct atcaataaaa
      961 cgaatatcga aatccaaaag ggaaatttta cttcttggtg ggagaacaaa acgttacaag
     1021 ataattttga actggcagaa aacaagaaac tccttaaaga aataggaagg ttgtcttatg
     1081 cagcaaaacg tagttcaaac tggtcaaata aagtagaaaa aagtaaatat ggaacaacaa
     1141 attctggttc aaaactggat aagggttatg ttggacataa ggctgcaaaa gcgatgaaac
     1201 gtgccaaaaa tattgagtca agacatcagg aagccgtttt acaaaaatca gaactgctcc
     1261 acaacattga acaatatgat gacttaaaaa tttcaccact tgaatttcac aaagagtgct
     1321 taatagaagc gaatgattta tcattgtctt atggagataa agaagtatgc agtaatctta
     1381 atttcagagt caatattggt gatagagttg ccattatcgg aaaaaatggg agtggtaagt
     1441 ctagtatcct aaaattgatt aatggagatg atattaaatt taccggaaat tttatgctag
     1501 caagtggact aaaaatttct tatatttcgc aagatacttc atatttaaaa ggtaatctat
     1561 ctgaatttgc ctataataat aagatcgatg aaactctatt taaaacgatt cttcgtaaac
     1621 tggattttaa tagagagcag tttgataaga acatggtgga ttttagtgct ggtcagaaaa
     1681 agaaagtact aattgctaaa agcctttgtg aaagtgcaca tttgtatata tgggatgagc
     1741 cattgaacta tattgatatt ttttcacgta tccaaattga aaaaatgatt ttggaatatt
     1801 gtcctacact attgtttgtg gagcatgatg atgctttttg caataacatt tgtacgaaaa
     1861 atattaattt aggtttgtag agattttgag ttgccacaat aactaaaaga tatttacatg
     1921 aaagggtgaa gaaatgttaa aacaaaaaga attaattgca aacgttaaga atcttactga
     1981 gtcagatgaa cgaattacag cttgtatgat gtatggatcg tttaccaaag gagaaggtga
     2041 ccaatactct gatatagagt tctatatatt tttgaaagat agtataacct cgaactttga
     2101 ttcatccaac tggttgtttg acgtagctcc gtacttgatg ctttataaaa atgagtacgg
     2161 aacagaggta gttatttttg ataatcttat acgtggggaa tttcatttcc tttctgaaaa
     2221 agatatgaac ataatcccct cgtttaaaga ttcaggttat attcctgata cgaaggctat
     2281 gcttatttac gatgaaacag ggcaattaga aaattattta tcagagataa gtggtgcaag
     2341 accaaataga cttactgaag aaaatgctaa ttttttgttg tgtaatttct ctaatctatg
     2401 gttgatggga atcaacgttc taaaaagagg agaatatgct cgttcattag aactcttatc
     2461 acaacttcaa aaaaatacac tacaacttat acgtatggca gaaaaaaatg ctgataattg
     2521 gctaaacatg agtaaaaacc ttgaaaaaga aattagcctt gaaaattata aaaaatttgc
     2581 aaagaccact gctcgattag ataaggtaga attatttgaa gcctataaaa attctttgct
     2641 attagttatg gatttgcaaa gtcaccttat tgaacaatac aacttaaaag ttacacatga
     2701 cattttagaa agattgttga attacattag tgaataggag agtaacgtta ttcacagtat
     2761 tgggtactga aaattaacag catacctcag tttttcaaaa aacagcgaag cctatggata
     2821 tgttcccgtc taccgatagt gcaaaaagtg tcgtggatat gtttccgaga acggacatac
     2881 acaaatgaca ttcattctgt cctctcaatc catgtggaag cagtatgtct gttaacaaga
     2941 aaataattat tgttcgtcct gacgagttgt tagactcgaa aaggataaac atcattttta
     3001 gaaccggtag ccgatttttg gctagcggtt ttttgggctc tttggcaaat agtggtgatt
     3061 gaatctcgcg ctatcctatg gaaaaccgcc ttgtgtgagg ggctgcgaga ccgcttcgat
     3121 cctagaattg ctgaagcacg aatctagcgt cactcacatt tcatcttttg acgtagctga
     3181 gacaaggtgt ttttcttcga tccgctagtg gttaatctct ttttggtgca aatatggttt
     3241 agaatttctt tgcatggctt aagggagtgg agaatcggat attagtagga tttacatcca
     3301 cagataaaaa taggcttgat tttaacagcg aaactaaaat accctactta aatgaagtga
     3361 tagattagat gttgggttgt taggagatgc ttaaaaatcc tatgcaatca atagttttta
     3421 gtgtttgttg acttgatccc taaatcgcct gagtactaaa cgttgagtgt gtctgcttac
     3481 tgtcactagc cagtaagtga gactgtagtt aatctctcat tttccccagt attatgttga
     3541 aaatagccat gaagcgatta tttctaaaga gttatttttg caggctcaag aagaaattta
     3601 tcggagaagt aatatctaca caggagaagg caagaacaaa agaatttata gtagcaagta
     3661 cgctttaagt gccatcacct tctgtggata ttgtggcgat atttatagga gaacctattg
     3721 gaatattcat ggcagaaaag aatttgtctg gcgatgcgtg actagaatcg agcagggtcc
     3781 cgaggtatgt aagaaccgaa ccgtaaaaga agatgaactc tatggtgcgg taatgaccgc
     3841 aattaataag ctacttgctg gtggcaacaa tatgataaag acactggaag aaaatattca
     3901 tgctgtgatt ggagaaacca cagaatacca aatttcagag attaataccc tactagatga
     3961 aaaacaaaaa gaactaatca agctggccaa taaaggtcaa gattatgaat acctggtaga
     4021 tgagatcgat gagatgcgag ataagcgtca gactctttta gtagaagatg cctccctcag
     4081 tggcgagaac gagcgcatca atgagttgat tgaatttatc cgcaagaaca aattccgcac
     4141 cttagaatat gatgataagc ttgtaaggaa gataatccag aacgttaagg tctatgaaga
     4201 ccacttcgtc atagccttca aaccaggtat cgaaatggaa atatgaaaac cagaagtaaa
     4261 tagcccatga ctctgaagta gagttgtggg ttttctttta attgctgatt ataataatat
     4321 tttaatgact attgcattta tcaaccaaat ggtttatgtt attcttagca taaacttgga
     4381 ggtgctgtat gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg
     4441 tttccgaact tgctagacgt attggccaga ctccacagaa ttttaataaa aaattacaac
     4501 gagaaacagt aaccttggat gagttgaagg ccattgctga tgtgctaggt gtcaagtttg
     4561 tgcaggcatt tattttacca gatggagatg aaataaaaat atctaacgaa taaaggagga
     4621 tgtttatatg gtaggtaatg ttattcaaat agtaacagag aaattatcat ctttgccttt
     4681 tatagaaggc attgtattag ggggctcacg tgcaagaagc acccatacag aaaattctga
     4741 tatagatatc ggaatctatt acaattcaga ctcatttgac ctgacagcga ttaatcaaat
     4801 tgctacagag ttggatgatg agaatagaaa caaccttgtt gtacctcccg gagcgtgggg
     4861 tgattgggtt aatggcggtg gatggttagt tataaacggg tgtcatgttg acttgatatt
     4921 acgtgatata aaacgggtgg aacaaataat caaagatacg gagcaaggaa ttgttactgc
     4981 caattatcag accgggcatc cccatggtta tataagtgct atgtatcgtg gagaattagc
     5041 gattagtaaa atactatatg ctaagaacga aagcttatgc gaattaaaaa agcaggcaga
     5101 aatttaccct actgctctaa agaaaagctt gatgaacttt tttatatttg aagctgaatt
     5161 ctctttaatg tttgtaaaag caaatgcggg ggcagaggat aaatattata ttgcaggcca
     5221 tgtttttcgt attatttcat gcttaaatca agtactattt gcatgtaata atgcttattg
     5281 cattaacgaa aagaaagcta taaaactgct tgaaactttt gaatataaac ctgaaaaata
     5341 tgccgaaaga gttaatcata tttttgaagt actcggtctt tctctttttg aatgctacga
     5401 catgaccgag aaactttata aagaagtgaa tgaaattgta tcggagataa ataacttttt
     5461 aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat
     5521 acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga
     5581 aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat
     5641 ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc
     5701 ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct
     5761 gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg
     5821 cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg
     5881 ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag
     5941 actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg
     6001 agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga
     6061 ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac
     6121 agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga
     6181 tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat
     6241 gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga
     6301 acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga
     6361 atttcaggat tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga
     6421 tgactggctt aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt
     6481 attcccacct gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat
     6541 tgatcttacc ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat
     6601 aaaggttcta attgataaag attgtagaat taaaagggac atagttccga ctgatataga
     6661 ttatcatgta agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa
     6721 tgtaacacct tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca
     6781 ttttaatcag attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga
     6841 aacaggcttt aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga
     6901 ggatttgtgg gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga
     6961 agcattattt ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct
     7021 tcattatgcc tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa
     7081 aaaatacact ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag
     7141 gcgggaacag tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc
     7201 gaaccatttg aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt
     7261 acagaattac tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag
     7321 gatgaggagg cagattgcct tgaatatatt gacaatactg ataagataat atatctttta
     7381 acccgtggtg ctagttaatt ttaatatcct aaagaat
//
LOCUS       DACEDM010000210         5934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.117, whole genome shotgun sequence.
ACCESSION   DACEDM010000210 DACEDM010000000
VERSION     DACEDM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.117"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1992
                     /locus_tag="IWO50_002971"
     CDS             <1..1992
                     /locus_tag="IWO50_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0360008.1"
                     /translation="PAPLIQIFLGVVLGLTEWGQSIDFEPELFLVMIIAPLLFREGEK
                     ADISSILKNFGTILFLAFGGVILTLVGVGATLSFLLPSVPLAACFAFGAALGPTDAVA
                     VSSLSGRVNIPKKAMHILEGEGLLNDASGVTAFQFALGALITGSFSAVNAGMSLVVSS
                     IGGALIGFLLVWFKQKIIHLIEKASAQDVTGYLLIELLLPFLAYVLAEAFDVSGIIAA
                     VAAGILQASGFRKISVFDAELSSLSHSTWTTIAFTLNALVFIFLGIELTQVFSPVWGD
                     GLYPNGLLLAIIVLISVMLFVIRFISISLFYVFKDGSKKFKKQLNEILILTFGGVKGT
                     VSLATIFILPFSINNMMFYQRSLLLFLTAGVILVTLVIGIIVLPMLTETEEAKSTDLN
                     ALMILEEVVEVLRKEIKEIDRNTKEFLATEAVIENYQERIRDLYLEDLTDDEKQEVQE
                     IQALILSIERDGLDESYRSGKLSSNGYRFYSRFLSRFEHSITSQILSFIGFWFIVVRR
                     LMRIVLHPKMFWQRRYADRQTFISDEDIQEIRETYQKNTQLIIESLDNLTDIYDETMV
                     QFFIQQRKLEGIKMNSGNLISSWMIQQDSLFTKKMLRGYYLERKIIDEYEVAEKITTF
                     SANSYRRNINLLESYTMNKPSDNFSFRFAFRAKSKKRVQ"
     gene            2157..4043
                     /locus_tag="IWO50_002972"
     CDS             2157..4043
                     /locus_tag="IWO50_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0360009.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4055..5002
                     /locus_tag="IWO50_002973"
     CDS             4055..5002
                     /locus_tag="IWO50_002973"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0360010.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5022..5549
                     /locus_tag="IWO50_002974"
     CDS             5022..5549
                     /locus_tag="IWO50_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0360011.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(5612..>5934)
                     /locus_tag="IWO50_002975"
     CDS             complement(5612..>5934)
                     /locus_tag="IWO50_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0360012.1"
                     /translation="TLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVV
                     AAVPLYHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSV
                     LFFT"
BASE COUNT         1970 a         1010 c         1283 g         1671 t
ORIGIN      
        1 ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa
       61 tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga
      121 gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg
      181 gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg
      241 ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg
      301 gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg
      361 gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt
      421 gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt
      481 attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg
      541 atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct
      601 tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca
      661 gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg
      721 agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata
      781 tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca
      841 aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc
      901 atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg
      961 aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt
     1021 tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt
     1081 ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg
     1141 acggaaacag aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc
     1201 gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc
     1261 actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta
     1321 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
     1381 gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac
     1441 tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc
     1501 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
     1561 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
     1621 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
     1681 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
     1741 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
     1801 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
     1861 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
     1921 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
     1981 cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt ataaaaaaca aaacatatca
     2041 gaacaggatc gaatcggtcc tgttcttttt ttgagaacac aaaaagaaca aataccaaaa
     2101 aaattttcct gaagttcact atttttatga tacaatatcc aagataagga gggaacatgt
     2161 tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg tgaaaaagat ttgtttgacc
     2221 agatttcttt tttggtccac gaaaaagacc gtatcgggtt gattgggacg aacgggacgg
     2281 gtaaaacaag tctcttaaat attattgccg gtatcgatag cagcgacggt gatcggcaaa
     2341 cagtgtttta tccaactgat taccgtattg gttatctttc tcaagcagct gaattttccg
     2401 atgaattaac tgttctccaa gcagtctttc aaggaaacag ccccttgatc caaactgttc
     2461 gagcatatga ggaagcattg atcgaactag gggaaaacgg agaagaccct gatgtgcaaa
     2521 aacgttatgc taaagcagaa gaacaaatga ataaagaaga tgcttggact acagatacca
     2581 acgcaaagat tattttgcaa aaattaggaa tcagtgaatt agataaaaaa atcagtactt
     2641 tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt gttgattgat gaacccgatt
     2701 tactattact agacgaacca acaaaccatt tagattatga agcgatcgag tggttggaaa
     2761 actatttgaa acaataccgt ggcgcactat tgatggttac ccacgatcgt tattttctag
     2821 atagagtagc aaaccgaatc ttcgaacttt catttggaaa attatacgag tataaaggaa
     2881 attatgaagc ttacgtctta gaaaaagcag aacgtgatcg tgtagaagta gaacaagaag
     2941 aaaagcgcaa gcggctttat aaacaggaat tagcttggat gagggctggc gtacaggcac
     3001 gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga tctaaaggag aacttgtacc
     3061 aagtaaacca agaagatgat ttagagctga acctagcgac acaacgttta gggaaaaaag
     3121 tactggaaat caaagatggc agttaccgta taaatgacca aacactgcta gaacatttag
     3181 atttattgat tcaatctcgg gaaagactag gtattactgg taaaaatggt gcgggaaaat
     3241 cgacattgct taatattcta gctggcagaa ttcccctgga tagcggtaca atgtctatcg
     3301 gtgaaacggt tcatcttgcg tattatacac aagaaaatga agaaatggca ccagataaac
     3361 ggatgattgc ttacttacaa gaagctgctg aagaagcaaa aacagcagat ggcagtcaaa
     3421 tcggagtagc agaattactt gaacgatttt tatttccaag atttatgcat gggacactga
     3481 taggcaaact ttcaggcgga gaaaaacgcc ggttattttt gttgaaactg ctgattcagc
     3541 aacctaatgt tttattattg gatgaaccga ccaatgattt ggacatagca acattgacga
     3601 ttttggaaga ttattttaga agttttccag gtgctgtgat cactgtttcc catgaccgtt
     3661 atttcttgga caaggtagca gataagttat tagtattcca aggaaacgga aaacaagaac
     3721 tttattacgg aaacatgtcc agttatttac tgaagcaaaa agagacccag cagcctgctg
     3781 aaaaagcgaa accaaaaata cagagcaagg aacctgctga aaagaagaaa ttatcttaca
     3841 tggaacaaaa agaatgggaa acgattgaag atgagattgc agaacttgaa gaaaaaatct
     3901 cattgctcca agaagagatg aatcatcaag gagataattt cactcgatta caagaattgc
     3961 aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa aatggcacgg tgggaatact
     4021 tgagtgaatg ggtagaagat taggaggaaa aaggatggaa caagcgtatc ttgacttagg
     4081 aagaaaattg ttggaagaag ggcatcgtaa agaagacaga acaggcacag gcacattaag
     4141 cttattcgga taccaaatgc ggtttgatct gcaaaaaggg tttcctttgt taacaacgaa
     4201 acgagtacct tttggattga tcaaaagcga attgctttgg tttttaaaag gagatacgaa
     4261 tatccgctat ctattacaaa ataaaaacca tatttgggat gaatgggcat ttgaacgcta
     4321 catcaaaagt ccagactata atggaccaga tatgactgat tttggccata gatgtttgac
     4381 agatcctgaa ttcaatgaag aatataaaaa gcagagcaag cttttttgcg agaagatcct
     4441 aaccgatgat tcctttgctg aaacatacgg cgatcttgga cacatctacg gctatcaatg
     4501 gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa atcaaacaag tgatcgaagc
     4561 aatcaaaaag actcctgatt ctagaagatt gatcgtttct gcatggaatc cggaagatgt
     4621 tcctagcatg gctttgcctc cttgtcatac gatgtttcaa ttttatgttc aagagggacg
     4681 gctaagctgc caattgtatc aacgcagtgg ggatgtgttt ctaggcgttc cgttcaacat
     4741 tgcaagttat gcgttgctga ctcacctaat tgcacatgaa acaggtttag cggttggcga
     4801 atttgttcac acattaggag atgctcatct ttatatcaat catatcgaac aaatgaaaga
     4861 acaattgagc cgagaaatac gcgcatttcc gactttagtt ttgaatcagg aaaagcagtc
     4921 agtatttgat tttgagatgg aagatatcca attagaagga tatgacccac atccggcgat
     4981 caaagctcct atcgctgttt agaatcagaa aggatgagaa aatgttagct gcaatttggg
     5041 cacaggatga aaacggattg attggaaaag aagaccgtct tccttggcgt ttgccaaatg
     5101 atctgaaatt cttcaaacaa atgacagaag caaatacact agtgatggga cgaaaaacat
     5161 tcgaagggat gggtagccgt ccgttgccga atcgtcagac gattgttttg acgagagatt
     5221 cttcctataa agcagaaggc gtgcatgtca tgcatagtgt ggaagacgtg cttgctctgg
     5281 aaaaagaaac agacggtatc tttttcatcg ctggagggtc agctgtttat caagaatttc
     5341 tgccattttg tacgatatta taccgtacag tcatccatca tgtattcgat ggagatgctt
     5401 attttccacc tgttgactgg tctgattggt cactgatcaa tactagtcaa ggagaaatgg
     5461 atgaaaaaaa tcgttatccg catcaatttg aaacgtatca gcgtaatgag aatcctgtaa
     5521 aaagcgggac aggcgaattg acgatataaa aaagctatgg atagactagc tgttatttag
     5581 agctgtccca tccatagctt tatttttttt attaagtaaa gaataaaaca gagaagtaca
     5641 taaaacctgc acctaacata acaaacaaat gccaaacaac atgcatatag cggatagaat
     5701 tcaagctata aaagaatgcg cctatcgtgt aagaaagccc gcctgctacc atcaaaataa
     5761 caccagtcat tccaagagag tgataaagag gaacagcagc cacgacacag agccacccca
     5821 taaaaatata gatgatcgta gatacttttg atactctctc ccgtttatgc aacgtcaacg
     5881 ctttatatat aatgccaaga atcgcaaaca accagattaa tacaaataaa gtcc
//
LOCUS       DACEDM010000211         4968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.123, whole genome shotgun sequence.
ACCESSION   DACEDM010000211 DACEDM010000000
VERSION     DACEDM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.123"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..170
                     /locus_tag="IWO50_002976"
     CDS             <1..170
                     /locus_tag="IWO50_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0360013.1"
                     /translation="PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVL
                     FWLDLPLTSTL"
     gene            341..1147
                     /gene="vanY"
                     /locus_tag="IWO50_002977"
     CDS             341..1147
                     /gene="vanY"
                     /locus_tag="IWO50_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0360014.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1165..1992
                     /gene="vanW"
                     /locus_tag="IWO50_002978"
     CDS             1165..1992
                     /gene="vanW"
                     /locus_tag="IWO50_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAQ0360015.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1989..2960
                     /locus_tag="IWO50_002979"
     CDS             1989..2960
                     /locus_tag="IWO50_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0360016.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2953..3981
                     /gene="vanB"
                     /locus_tag="IWO50_002980"
     CDS             2953..3981
                     /gene="vanB"
                     /locus_tag="IWO50_002980"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0360017.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            3987..4595
                     /locus_tag="IWO50_002981"
     CDS             3987..4595
                     /locus_tag="IWO50_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ0360018.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1402 a         1055 c         1309 g         1202 t
ORIGIN      
        1 ccccattcta tcgcattgat caggcgcgaa gcagaaaaag tgggcgaagc ggtttaggac
       61 ttgccatcgt acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg gaaaacacct
      121 cggatggcgt tttgttctgg ctggatttac cgctcacatc aacattgtaa atatttaaaa
      181 cttaaatgat tttgaccgac aggtataacc ctgccggtct ttttttgctg ctacaggaaa
      241 actacagact ggctacaggg aaagtacaga tgcgcttgcc ataataacaa tcgtaccagc
      301 cacaaatcgt agttttattg caaaggaggc attcgatcaa atggaaaaaa gcaactatca
      361 ttccaatgcg gatcatcaca aacggcatat gaaacaatcc gtggaaaagc gggcttttct
      421 atgtgcgttc attatttcgt tcacagtctg cacgctgttt ttgaggtgga gactggcttc
      481 cgtattggag gcaacacaga taccgcccat ccctgcaact catacaggca gcagcactga
      541 cgtagtggag aatttggagg aaaacgctct tgccaccgcc aaagaacagg gagatgaaca
      601 ggaatggagc ctgattttag tgaacaggca gaaccccatc cccgcacagt acgatgtgga
      661 gcttgagcaa ctatcaaatg gtgagcggat agatattcgg atttctccct atcttcaaga
      721 tttgtttgat gccgcaagaa ctgatggagt ttacccgatt gtcgcatccg gataccgaac
      781 aacagaaaaa cagcaagaaa ttatggatga aaaaattgcc gaatataagg cgaaaggcta
      841 cacctctgca caggctaaag cggaagcaga aacttgggtg gccgtgccgg gaacgagcga
      901 gcatcagctt ggtcttgctg tggatatcaa tgcggacgga attcattcaa caggcaacga
      961 ggtttataga tggctggatg aaaacagcta tcgttttggt tttattcgtc gctacccacc
     1021 agacaagaca gagataaccg gtgtgagcaa cgagccgtgg cattaccgat atgttggcat
     1081 cgaagctgcc acagagatgt acaaccaagg ggtttgcctt gaggaatatt taaaaccaga
     1141 aaaatgagaa aaggatgtta tgttatggac agaaaaagat tgacacagcg tttcccgttc
     1201 ctgcttccaa tgagacgggc gcagagaaaa atgtgctttt atgcgggaat gagatttgac
     1261 ggctgtcgct atgcgcagac gataggggaa aaatcgcttt cccatttgct ctttgaaacg
     1321 gattgcgcat tatacaacca caataccgga tttgacatga tataccagga aaacaaggtg
     1381 ttcaacttaa agctggcagc aaagacctta aacggcttat tgataagacc gggggaaacc
     1441 ttttctttct ggtggctggt acgccatgcg gacaaagata ccccctataa agacggcctt
     1501 acggtgacca atggtaaact caccaccatg tcgggcggcg gtatgtgcca gatgagcaat
     1561 ttactatttt ggatgttcct gcatacgcca ttgacaatta tccagcgcag aggtcacgaa
     1621 gtaaaggagt ttccagagcc aaacagtgac gagattaaag gggtggatgc aaccattcca
     1681 gagggctgga ttgatttaaa agtgcgaaac gataccgact gcacctacca aatatgggtg
     1741 accttagatg atgagaaaat catcggtcag gtgtccgccg acaaagagcc ccaagcatta
     1801 tacaaaatta caaatggcag tatccagtat gtccgtgaaa gtggcgggat ttatgaatat
     1861 gcccaggtta aacggatgca agttgcctta ggtaccgggg aaataataga ttgcaagctg
     1921 ctttatacaa acaaatgcaa aatctgttat cccctcccgg aaagtgtgga tattcaggag
     1981 gagaaccaat gagaaaaagt gtgggcatta ctgtttttgg atgcgaacag gatgaggcaa
     2041 atgctttccg cgctttatcg ccggattttc atattatccc tacgctgatt agcgatgcga
     2101 tatcggcaga caacgcaaaa ttggccgctg gcaatcaatg cgttagcgta ggccataagt
     2161 ccgaggtttc cgaggcgaca attcttgcgc tgagaaaggt cggggtaaaa tacatttcta
     2221 cccgcagcat cggctgcgat cacattgata cgactgccgc cgagagaatg ggaatctcgg
     2281 ttggcacggt tgcgtattcg ccggacagcg ttgcggatta tgctttgatg ctgatgctga
     2341 tggccatacg gggtgcaaaa cccaccatgc acgccgtggc gcaacaagat ttcagattgg
     2401 atcgtatccg ggggaaagaa ctgggggata tgactgtggg agttattgga accggccata
     2461 tcgggcaagc ggtcgtcaaa aggctgcggg gatttggatg ccatgtgctg gcctatgata
     2521 acagccgaaa aatggatgca gattatgtcc agcttgatga gcttctaaaa aacagcgata
     2581 ttgttacgct ccatgtgccg ctttgtgcgg atacccgcca tctgatcggt cagaagcaaa
     2641 ttggagagat gaagcaaggc gcatttttaa tcaacaccgc gcgcggagcg cttgtcgata
     2701 ccggggcgct ggtggaggcg ttggaaagcg gaaagctggg tggtgcggca ctggatgtgt
     2761 tggagggcga ggatcagttt gtttataccg attgctcgca gaaagtgctt gaccatccct
     2821 ttttgtcgca gcttttaagg atgccgaatg tgatcatcac accccatacg gcgtactaca
     2881 ctgagcgtgt gctgcaggat actacagaaa aaacaatcag gaattgtctc aattttgaaa
     2941 ggagtttaca gcatgaatag aataaaagtc gcaatcatct tcggcggttg ctcggaggaa
     3001 catgatgtgt cggtaaaatc cgcaatagaa attgctgcga acattgatac ggaaaaattc
     3061 gatccgcact acatcggaat tacaaaaaac ggtgtatgga agctatgcaa gaagccatgt
     3121 acggaatggg aagccgacag tctccccgcc atactctccc cggataggaa aacgcatggg
     3181 ctgcttgtca tgaaagaaag cgaatacgaa acacggcgta ttgatgtggc tttcccggtt
     3241 ttgcatggca aattcgggga ggatggtgcg atacaggggc tgtttgtatt gtctggtatc
     3301 ccctatgtgg gctgtgatat tcaaagctcc gcagcttgca tggacaaatc actggcctac
     3361 attcttacaa aaaatgcggg catcgccgtt cccgaatttc aaattattga taaaggtgac
     3421 aagccggagg cgggtgcgct tacctaccct gtctttgtga agccggcacg gtcaggttcg
     3481 tcctttggcg taaccaaagt aaacggtacg gaagaactta acgctgcgat agaagcggca
     3541 ggacaatatg atggaaaaat cttaattgag caagcgattt cgggctgtga ggtcgggtgt
     3601 gcggtcatgg ggaacgagga tgatttgatt gtcggcgaag tggatcaaat ccggctgagc
     3661 cacggtatct tccgcatcca tcaggaaaac gagccggaaa aaggctcaga aaatgcgatg
     3721 attacagttc ccgcagacat tccggtcgag gaacgaaatc gggtgcagga aacggcaaag
     3781 aaagtatatc gggtgcttgg atgcagaggg cttgcccgtg ttgatctttt tttgcaggag
     3841 gatggcggca tcgttctaaa tgaggtcaat accatgcccg gttttacatc gtacagccgc
     3901 tacccacgta tggtggccgc cgcaggaatc acgcttcctg cactgattga cagcctgatt
     3961 acattggcgt taaagaggtg ataagtatgg aaaatggttt tttgttttta gatgaaatgt
     4021 tgcatggcgt ccgttgggat gccaagtacg ccacatggga taacttcaca ggaaaaccgg
     4081 tggatgggta tgaggtgaat cgcatcatcg gcacaaaggc cgtggcgttt gctctgcgcg
     4141 aggcacaaat ccatgcggct gcgcttggct atggcttgct tttatgggat ggatatcggc
     4201 caagaactgc ggtggactgc ttcctgcgtt gggcagcgca accggaggac aatctcacaa
     4261 aagaaaaatt ttaccccaat atagagcgag ccgagttgat tacaaagggt tatgtggcct
     4321 cacaatccag ccatagccgt ggaagcgcaa ttgatcttac gctctaccac ctggatacag
     4381 gggaacttgt ttcaatggga agtaacttcg attttatgga cgaacggtcg catcatacag
     4441 caaaagggat aggggatgca gaggcacaaa atcgaagatg cttgcgtaaa atcatggaaa
     4501 gcagcggatt tcagtcttat cgctttgaat ggtggcacta taagttgatt gatgagccat
     4561 accccgatac ctattttaat tttgccgttt cataatgaaa gtattggttt tgtatcagat
     4621 tttggatttg ataaaatcta tgctggtcat ggacaatctt ttactggaaa gtggttaaag
     4681 catcttttgt aatacattgc aaattatagt attcaatctg cccagcggca gaaaagaaaa
     4741 aaaccgctgt tgggcagacc tttcccacgc ccattttgta ttttgctggc ggcggttttg
     4801 agaaatcaag gccgccgttc ttttctggca gcaaaaggtg tggcggccca taggaggatg
     4861 ccgctatgct gttttgctat tgccatgctc taatccactt ccaccactca aaaaagcctt
     4921 gcccctccat gatgtgcacc ccaaaagtta gactagaaat ctaattaa
//
LOCUS       DACEDM010000212         4261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.130, whole genome shotgun sequence.
ACCESSION   DACEDM010000212 DACEDM010000000
VERSION     DACEDM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.130"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            150..647
                     /gene="dfrG"
                     /locus_tag="IWO50_002982"
     CDS             150..647
                     /gene="dfrG"
                     /locus_tag="IWO50_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0360019.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(719..2671)
                     /locus_tag="IWO50_002983"
     CDS             complement(719..2671)
                     /locus_tag="IWO50_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0360020.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2678..2974)
                     /locus_tag="IWO50_002984"
     CDS             complement(2678..2974)
                     /locus_tag="IWO50_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0360021.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3152..4261
                     /locus_tag="IWO50_002985"
                     /pseudo
     CDS             3152..4261
                     /locus_tag="IWO50_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1333 a          727 c          705 g         1496 t
ORIGIN      
        1 tgaatgaaaa tttgtgattt tgtggtatta tgataatata acttatagaa aaaattgctt
       61 attcaactaa cgggcaggat aatgagaaaa ggttttttga tagaaataag aaattagatg
      121 gtaaattagt taaaaaggct gtgttataaa tgaaagtttc tttgattgct gcgatggata
      181 agaatagagt gattggcaaa gagaatgaca ttccttggag gattcccaag gactgggaat
      241 atgttaaaaa tactacaaag ggacatccga taatattagg taggaagaac cttgaatcaa
      301 tcggaagagc cttacctgac agaagaaata ttattctgac gagagataag gggtttacct
      361 ttaatggttg tgaaattgtt cattcaatag aagatgtttt tgagttatgt aaaaacgaag
      421 aagaaatttt tattttcgga ggagaacaga tttataattt gtttttccct tatgttgaga
      481 aaatgtacat cacaaaaata catcatgaat tcgaaggaga tacttttttt ccagaagtga
      541 attatgagga atggaatgag gtatttgccc aaaaagggat aaagaatgat aaaaatccgt
      601 ataactacta ttttcatgta tatgaaagaa aaaacttatt gagttaacgg gtgcgttaat
      661 tgaagaagaa cagtaaagat aaagcacaag agcttgggat tactccaagc tctttttgtc
      721 atttgaaata aattatatct ttccaatcaa attgtttatc tgtaatccct aatctttgag
      781 caggtgtcaa aaccttttta tccgctgatt taaaagggcg acaaaagttg tagtaagttc
      841 ttagaattgt aatcgcaaat tgagcatact tcggattgaa gttagcgtaa atatagctct
      901 tcttatcacc acgagctgtt gttaatggtc tttcaagaga tgatatataa cgtcttatct
      961 gttgaataaa gctattggtc gaatggtcat taacatttaa tagcatcttt gcgatttcgt
     1021 ttggttccag agcagataaa tccgttctac aatcaactga ataaaatcct ttatccttag
     1081 tggctaatgg atgtttgata gggttatcgg catatttgcg atatctttct ccatctttac
     1141 taatagcctc ttcgtggaat tgatgacttg ttagtaattc agttagataa tgagatgcca
     1201 aagtccgtag aaactttgtt ttgaatcctc tgatatctcc ccaatcgagc agttctgctt
     1261 ttgcttgtcc gaattctttt aagcattgtt ttctgctttt cgtcttgtta acttggcaaa
     1321 taaaatgatg tgcatcgctc aaccttaatt ctttcgtgaa tactctataa aaggcgtttc
     1381 taatagagaa gtcatcatca ctaatcattc gccattcgtc ggcattaacc atttgtttaa
     1441 ttaaccaata gtgagcagtt gtagtataag gagcatcaac atttaaacca tcgacaaatc
     1501 tactcctatt agtaattttc cctaattcgt gaagataagc attcctattt tcgctatcgt
     1561 tagcggatgg ttcttgtgga taataagacc aatctaacct atcattttta cgactaaaag
     1621 tattgagatg gtcttctttg aattttcttg tatcttcatt taattcatcc attgaaatat
     1681 tccaatcata ggcaacatca gaacggaaca catatcttga taagacctca gcagatacaa
     1741 ctatatacgt ttgcatatta aggtcttcaa accccgtata tttctttgaa ccttgtcctt
     1801 tcttacggac gttatttagg tagtagtgca tcttgtccgt attaagccac atctcgttaa
     1861 atttctttgt ttgaaggggt tgtgtctcat atcgttctaa aaactccaaa caacgcctat
     1921 acagccactc cagcttatga tagtaggttc caactccaat tcctaaaata tcacaagtac
     1981 gactgactga aacccttcca acaaccatct tagcgaacat tggaagaatg gtgttctttt
     2041 gttggtgata agtagtggtt tcttctcttt ttggtagaac attagtgaac ttcttacacg
     2101 ccttacattg atagcgttga gatttgcccc tacttttccc ccttttgtaa aattgcttcg
     2161 gctcattgaa tggggttgat tcttcttccg agcagccttc tttatgaaat tgataatctg
     2221 gctcaacatc ttggatgcta ttgatttcta taaggcgttt aatttcttca acaacagacc
     2281 aattagataa aggggttact gtacagtttt gcgatacacc tctgccaata ggattgtcat
     2341 tgcagtacat acctttatga cctttatctt tggaactacc agtcatttta tatcgacttg
     2401 gtttcccttt tacatctttg aatttgtgct gttctttccc aaaattacaa caaaacggat
     2461 tgatgcagta attatattga atgggatatt cagaaccgtt ccatttaaaa gacgttggca
     2521 agaacaacat tttgttatac cttgttgcaa atcgttttgg agttaataaa tcatattgtt
     2581 tagccctatc ttttaattct tcgtttgaaa tcggaatttt aacatctaca atttcatctt
     2641 ccttcgtggc taatctttta agcttggtca ttgtctatca acccactcta tctttgatac
     2701 ccattttttc aacaatccgc ttattacttc gcttgttggc aatccattca ataaacttct
     2761 tatcgtctaa aatgttcaat aagaattcat ccactgcttc gctttcagta cgctctgcat
     2821 attcagcgta ttgcttaact atcaccctta cttgctctga tatttcccaa tccacagact
     2881 tagcgttctt attttttggc tcaagaaatt gcataggaaa atgcacccct ttttgcttag
     2941 taaattgtaa ccattttaac acaccaattt gcattcctac aataattatt tcacgttcgc
     3001 atttttatat acattttaac ttacattttg tggtgtaaat taacaacaaa cgttgattta
     3061 acaataaaaa acgagtatca aattcagttg caatttgata ctcatttagg tttacatttt
     3121 cactttttgt actccaaaac tgaactactt atttttattt agttgtctct atattttctc
     3181 tgcttatcgg acagtagaca tctactatcg ttccgcaatt gccgaagcta tcgcaataac
     3241 acttgttatc cctgtattat tttatgctta tcaaatcatc tctggaaaag aagaaaagta
     3301 tccgtctgta aagctagcat tgagtatgag tttgctagtg tacagccatg tactatcaac
     3361 attaatgagt acagcattaa ttatcatttt tatttttatt cgtttggttt ctaaaggttt
     3421 caagaatgca gattttattg ctatatttaa aaaattattt agtgcggcag gaatgacatt
     3481 ggttctgacg agtgcttttt ggtatccgat gtttgaacaa atgctctacc aaaaaatcaa
     3541 caaacctagc gtgacaaatt tatatgctca tgcatctaat gtgttcgata gtctgactga
     3601 agctatgaac aacgacttaa ctacttactc aatgggacta gttggtctgt tatctttatg
     3661 tattcccctg attttattta aaaaattaac aaacatagaa aaaaagattt attatggtac
     3721 gtgtttgact tggctagcta caactagttt ggtgccatgg tatcttctgc aaaatacgcc
     3781 agccaaattg ttacaatttc cttggcggat cttaagcttg caaataatat tctcttctct
     3841 gatattaacg atgatatttt tcaaaaatag aagatacaat aagacaagag aactatttta
     3901 tttatttatg actgttttgt tattaatcac tttaactgtt tctagtaaaa aaaatttcaa
     3961 tcaaaaaata atttctcagc cgggacatat tgttgtaaac aaagaaacag tagaggcgta
     4021 taccactaaa ggaatggatt attttttata tgattattct cctatcgctt cattggagaa
     4081 taaggagcat ataataaaac acgaagcaaa gattggcagt caatgggtaa accttcctta
     4141 taaaacaact ggtagttcta tcatatatag agtaactaat gagaagagcc aagtaatcac
     4201 acttcctgta tatgcttatt taggaacgaa tgtagaaata aatcatcaac ctgtaaagga
     4261 g
//
LOCUS       DACEDM010000213         4117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.134, whole genome shotgun sequence.
ACCESSION   DACEDM010000213 DACEDM010000000
VERSION     DACEDM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.134"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            67..1545
                     /gene="msr"
                     /locus_tag="IWO50_002986"
     CDS             67..1545
                     /gene="msr"
                     /locus_tag="IWO50_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0360022.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1592..2038
                     /locus_tag="IWO50_002987"
     CDS             1592..2038
                     /locus_tag="IWO50_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0360023.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2237..2752)
                     /locus_tag="IWO50_002988"
     CDS             complement(2237..2752)
                     /locus_tag="IWO50_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0360024.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2869..4071
                     /locus_tag="IWO50_002989"
     CDS             2869..4071
                     /locus_tag="IWO50_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0360025.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1224 a          702 c          894 g         1297 t
ORIGIN      
        1 cagatttttc tgctcttttt tagtatcgat aattgttgtt ttacatgaaa taggagtgta
       61 aaacatatgg aaaatttagc agtaaatata acaaatctac aagtcagctt tggcaaccag
      121 ctagaactat ctattgattc tcttcgtgtc tatcagcaag atcgtatagg gatcatagga
      181 gaaaatggtg tcggtaaatc gactttgctc aaactaatag ccggtgaact ttttcccgat
      241 catgggaaaa tccaaacaga gatcaccttc aactacctgc ctcaattaac ctatctcgct
      301 gaagcaaagg acctaaattt ggaattagcc agtcatttcc agttaagact ggaagaaact
      361 tcagagcgga aatggagcgg aggggaagaa cgaaagatcg agttgatacg tcttctttct
      421 tcttatgaac aagggatgct tctagacgag ccaacaaccc atctagatag aaaaagtatt
      481 gatcgactga ttgaagagct tcgttattat tatggtacgc tggtttttgt tagtcatgat
      541 cgctattttc tagatgagct agcatcgaaa atctgggaag taaaagacgg agaaatccga
      601 gagttttcgg ggaattatag tgcctatctc actcaaaagg aattggagaa aaagactcag
      661 ctacgagaag cagagtcgat catgaaagag aaaaaacgat tggaaaaatc gatccaagaa
      721 aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa gcaacaaatc
      781 agactggatc ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc catccaaaag
      841 aatgcgaaaa cattagagag aagactccaa aaaataggag aaacaaccaa gccgcagcag
      901 atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac tccacagccg ctatcccatc
      961 atgggacaaa atgtccaatt ggaaagaagt gggagaacat tactggtaaa tggtgatttt
     1021 cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg taagacaacc
     1081 ttattagaac atatccgcaa acaaggagaa ggaatccttc tctctccgaa agtaagcttt
     1141 caagtatatc agcaaaaggg ttatcaaatg acatctgaag aatccatcat tcgttttgtc
     1201 atgagacaaa cagagttttc agaatcgctt gtccgtagtt tgctgaatca cttagggttt
     1261 gctcaggaaa ctttgacgaa accgttatgt acattaagtg ggggagaagc gacccgtctg
     1321 acgattgctt tgctttttac taagccaagt aatgtgttgc tgttagatga accgactaat
     1381 tttattgata tggcaacgat cgaagcttta gagaagctaa tgcaaatata tccgggaacg
     1441 attttgttta cttctcatga ttcttacttt gtcgagcgta cggctgatga agtttatgaa
     1501 ataaaagggc agaaaataaa aaaagtactt acgagaaatt tttaaatgtg ctacgatgat
     1561 tttcgtgaca aatttaaagg agtggtacag attgcctaca aataaaaaag aaggaataat
     1621 ttttacaaca atgatgtgtt ttttgatggt cttggggatg agcgcgtata atttatggct
     1681 gcatcaagat cttcatttat caagtttgtt gattggtcta gtgcctggat tcgtggtggc
     1741 tttcattttg gatgtcttca tcatcggtgt gctggcgaaa aaaattgcct ttaaactacc
     1801 agtaaatcca gaaagcaagg tccaaatgat cttaactatt tcatgcttga tggttttagg
     1861 aatggtgaca tgtatgtctt tattcggtgt gatcatggaa caaggaatac ctgatcaatt
     1921 attctctgct tatatgaatg cttggaaaat gaatgtgatt gccgctttac cgttgcagtt
     1981 gatcatcgtt ggcccgcttt caagaagtgt gctaaaagca attcaaagta atgaatgatg
     2041 gaacactgtt atttgaaaaa aggccttggc atgctttttg tcatagtctc ttgaatgcag
     2101 ttctagaaaa atagtgatcc gaacgatagg ttttggcctg tcgttcggat cgctttttct
     2161 tttccaactg tattgctctt ttattgaaga taatttgctc ttcaatagat atttctagaa
     2221 aggttctgtt tatcccttac tcaaaaaact gctgcatttg ttctcttact tgctgacacc
     2281 attggagttc ctgctgacaa agcataattc tgcgctggat cacaacataa cgtccaaatt
     2341 cttgtttctt gtctagcgag aggtcattca attttttttg atttttttgc atgattttgt
     2401 tcagctgttg ttctctagtg aatagcagtc cagtagctgt tgttttatcg agcgtcgaaa
     2461 aagcatagat ttttgctaga aattcatctt tttgcgtagg agcgggagtt tcctctttga
     2521 tccattcctc tacaagagca cgtccttcat cagtcaagtg gtagatcttt ttttgagagt
     2581 gctcgtcttt gtccaaaacg gtcacataac cctgctggtc taacttggca agcgttgtat
     2641 agatctgact gtgatgggct tcccaaaaaa ttgtgatcag ctgcttcaat tcatacccag
     2701 aacgtggttt ggataaaagc acgcataata atatataggc taaagtattc ataaaaatcc
     2761 tacctctcgt cttattttct ttattatatc acagtaaagt ttccacgttt tacatgtgca
     2821 tattgacatg taaaataaat catgtatgat gctaatagag agaaagggat ggaaatattg
     2881 gagaatcaaa tagatataca agaacaaaca aatgaagaaa caaaggggaa aagttcagcg
     2941 ctagttgtag cagctacctg tatgatcgct tttatgggag tgggtctagt agacccaatc
     3001 ttaaaaacga ttgctgcaca gttaaacgct acgccagctg aaacgaccct gttatttact
     3061 agttacatgt tagtcactgg tgtggtgatg ctatttacag gtttcttatc tagtcggatc
     3121 ggtttgaaaa aaacattgat gacaggattg tttatcatcg ttgtattcgc tggtcttggc
     3181 ggattatctg ggaatatcgg aatgctgatt gggttacgtg ccggctgggg tgtcgggaat
     3241 gctttatttg tctctactgc tttagcaacg atcgtcagta ttttagttgg taacacagaa
     3301 aaagcaatca tgatgtatga agcagcactt ggctgcggaa tggctgttgg ccctctagtc
     3361 ggtggggtct tgggcagtgg ttcatggcgt tatccatttt ttggggtagc atttttgatg
     3421 ttttgcggtt ttatagccct agcggtactt ttacctgaaa ctgagaaacc aaagagaaaa
     3481 gtaggccttt gggaaggtgt caaagcgtta agcaatcata aacttcgttc agttggacta
     3541 atcgcattgt tatataattt cggattcttt actttacttg cgtatgcgcc atttttattg
     3601 attggctatt ctgaattaga agtcggtttt gtattttttg gctggggtgt gcttctggct
     3661 attgcttcga ttttcgttgc accggctctt gagcggaagt ttacgactta tcggacaatt
     3721 ttagcagcat ttgtcttatt cattatttgt ttgctgttat taggagctgg aacttctatg
     3781 ccagtccttg taccgatcag cattgtgtta gcaggatttt tccaaggaat tatcaataca
     3841 ctgttgacga cgattgctat ggaaatacca ggtctgcaac gaaacgttgc ttcgtccagc
     3901 tatagttttg tccgcttttt cggtggagct ttagcaccat ttattgcagg tgtgattggt
     3961 gaacatattg gtgaaagcta tgcgttttat tttgcaggtg ccattgttct tatcagtgta
     4021 tgttgtatca tttatcaccg taattatttt gctacaaatg aagaagaata agaaatcaga
     4081 ggttgtgaaa aagctgtcct gcatcaagtc ataagaa
//
LOCUS       DACEDM010000214         3199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.143, whole genome shotgun sequence.
ACCESSION   DACEDM010000214 DACEDM010000000
VERSION     DACEDM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.143"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..78
                     /locus_tag="IWO50_002990"
     CDS             <1..78
                     /locus_tag="IWO50_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0360026.1"
                     /translation="QTKATQVSQFDLVLEKNGSNWKIIE"
     gene            323..439
                     /locus_tag="IWO50_002991"
     CDS             323..439
                     /locus_tag="IWO50_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0360027.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            455..2374
                     /gene="tet(M)"
                     /locus_tag="IWO50_002992"
     CDS             455..2374
                     /gene="tet(M)"
                     /locus_tag="IWO50_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0360028.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2493..2660
                     /locus_tag="IWO50_002993"
     CDS             2493..2660
                     /locus_tag="IWO50_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0360029.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2720..3073)
                     /locus_tag="IWO50_002994"
     CDS             complement(2720..3073)
                     /locus_tag="IWO50_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0360030.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
BASE COUNT         1090 a          487 c          616 g         1006 t
ORIGIN      
        1 cagaccaaag caacgcaggt atctcaattt gatttggtac ttgaaaagaa cgggagtaat
       61 tggaagatta tagaataaca aatattggta cattattaca gctattttgt aatcacgtac
      121 tctctttgat aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa
      181 gtatctattt aaaaggagtt aataaatatg cggcaaggta ttattaaata aactgtcaat
      241 ttgatagcgg gaacaaataa ttggatgtcc ttttttagga gggcttagtt ttttgtaccc
      301 agtttaagaa tacctttatc atgtgattct aaagtatccg gagaatatct gtatgctttg
      361 tatgcctatg gttatgcata aaaatcccag tgataagagt atttatcact gggattttta
      421 tgcccttttg ggcttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt
      481 agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata acagtggagc
      541 gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata cgcttttaga
      601 acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg aaaatacgaa
      661 ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat atcgttcatt
      721 atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac aagcacaaac
      781 tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt ttatcaataa
      841 gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag agaaactttc
      901 tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatgtgtgtg tgacgaactt
      961 taccgaatct gaacaatggg atacggtaat agagggaaac gatgaccttt tagagaaata
     1021 tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca taagatttca
     1081 gaattgttct ctgttccctc tttatcatgg aagtgcaaaa agtaatatag ggattgataa
     1141 ccttatagaa gttattacta ataaatttta ttcatcaaca catcgaggtc cgtctgaact
     1201 ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg catatatacg
     1261 cctttatagt ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat
     1321 aaaagttaca gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta
     1381 ttctggagaa attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga
     1441 tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac
     1501 tgttgaaccg agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc
     1561 agatagtgat ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc
     1621 tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt
     1681 ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga
     1741 atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt
     1801 atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt
     1861 aaatcaatca tttcaaaatg cagttatgga agggatacgc tatggttgtg aacaaggatt
     1921 gtatggttgg aatgtgacgg actgtaaaat ctgttttaag tatggcttat actatagccc
     1981 tgttagtacc ccagcagatt ttcggatgct tgctcctatt gtattggaac aagtcttaaa
     2041 aaaagctgga acagaattgt tagagccata tcttagtttt aaaatttatg cgccacagga
     2101 atatctttca cgagcataca acgatgctcc taaatattgt gcgaacatcg tagacactca
     2161 attgaaaaat aatgaggtca ttcttagtgg agaaatccct gctcggtgta ttcaagaata
     2221 tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg
     2281 gtaccatgtt actaccggtg aacctgtttg ccagccccgt cgtccaaata gtcggataga
     2341 taaagtacga tatatgttca ataaaataac ttagtgtatt ttatgttgtt atataaatat
     2401 ggtttcttgt taaataagat gaaatatttt ttaataaaga tttgaattaa agtgtaaagg
     2461 aggagatagt tattataaac tacaagtgga tattgtgtcc tgtatgtgga aataaaacac
     2521 gattaaagat aagggaagat actgaattaa aaaaattccc cctctattgt ccgaaatgca
     2581 gacaagaaaa tttaattgaa ataaagcagt tcaaagtaac tgtgattaca gagccagacg
     2641 caaagacgca gagccgataa aatgagatta atacaatctc attttatcgg ctctttccgt
     2701 tatgtatgga ttcttttaat tagtcttcga tgtttcttgc ttcgttgata ccgctggcta
     2761 aagattccat tatggatagt tctttgtctg taaagctatc catgtatttc tctatctgta
     2821 atcgtcgggt gctttttacc aagttattag caggtaagaa aaattcatca acggaaacat
     2881 gaagtaacga tacaaggtca taaagaactt gtatgctggg gtgttgccct ttattttcaa
     2941 tattagttaa gtaccgtggg tcaatttcaa tcaatgctcc cacttgttca cgagttaaac
     3001 ctcgtttcaa tcgagcttct ttaatggcta aaccaaaggc tctaaaatca tatttatctt
     3061 cttttttacg catagtagac cacctctata cattttattg ttcctactga attaaaaaca
     3121 ggtatagaaa aacgtgttat atggtttata ggtttatatt taataaaaag cactactaaa
     3181 cgccaataaa aaaaaccgt
//
LOCUS       DACEDM010000215         2291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.156, whole genome shotgun sequence.
ACCESSION   DACEDM010000215 DACEDM010000000
VERSION     DACEDM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.156"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(22..1125)
                     /locus_tag="IWO50_002995"
     CDS             complement(22..1125)
                     /locus_tag="IWO50_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0360031.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1678..2226
                     /gene="aac(6')"
                     /locus_tag="IWO50_002996"
     CDS             1678..2226
                     /gene="aac(6')"
                     /locus_tag="IWO50_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0360032.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          727 a          403 c          449 g          712 t
ORIGIN      
        1 gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc tttcttgtgt
       61 agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg tgatttcata
      121 aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt ctaaatcggt
      181 cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt tcataaacac
      241 atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca gtacaccata
      301 actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg taccgtaata
      361 gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa ccaatccggt
      421 agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat cgtcggcact
      481 atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt ttccgatttg
      541 gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag tagattcgac
      601 ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg cttcggcaat
      661 aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat
      721 tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat
      781 agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta cagtatcgca
      841 agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt catgctggat
      901 caactgttca atatttccat aagttgaatt acccccgtac cagagactgg cgacaacctc
      961 tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg cagtcttccc
     1021 tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt tttcatacgc
     1081 atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca tttccttctt
     1141 tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg ctgtagatgg
     1201 agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac accttgaaat
     1261 catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac aataatgaat
     1321 ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatta tctaaaaatt
     1381 ctaaaaatta agattattat atgaaaacag gttttatttg tcaattcata attggttttt
     1441 tttaaagcaa aagataatct atatcatgat gaacaaacag agttttttcc tccaattgat
     1501 ggctttcctg atctttccta ttttgaataa agtgtttctg tataattttt ttattttata
     1561 agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag
     1621 aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg
     1681 ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg
     1741 agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg
     1801 aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt
     1861 gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc
     1921 cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc
     1981 agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt
     2041 caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa
     2101 catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca
     2161 aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct
     2221 caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac
     2281 gaaaattact t
//
LOCUS       DACEDM010000216         1970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.162, whole genome shotgun sequence.
ACCESSION   DACEDM010000216 DACEDM010000000
VERSION     DACEDM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.162"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..439
                     /locus_tag="IWO50_002997"
     CDS             47..439
                     /locus_tag="IWO50_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0360033.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="IWO50_002998"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="IWO50_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0360034.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          819 a          149 c          337 g          665 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaattc aaaatctttc agagaagctg ttaaaggaat
//
LOCUS       DACEDM010000217         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.164, whole genome shotgun sequence.
ACCESSION   DACEDM010000217 DACEDM010000000
VERSION     DACEDM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.164"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWO50_002999"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWO50_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWO50_003000"
     CDS             394..477
                     /locus_tag="IWO50_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0360035.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO50_003001"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO50_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0360036.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACEDM010000218         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51503_2017
            SAMN12640095-rid9879683.guided.173, whole genome shotgun sequence.
ACCESSION   DACEDM010000218 DACEDM010000000
VERSION     DACEDM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640095
            Sequence Read Archive: SRR10023206
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741731.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:07:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,003
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,875
            CDSs (with protein)               :: 2,875
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 12 of 66
            Pseudo Genes (multiple problems)  :: 11 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640095-rid9879683.guided.173"
                     /isolate="E51503_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWO50_003002"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWO50_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWO50_003003"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWO50_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0360037.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
